LOCUS       DAZUMO010000001       145407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000001 DAZUMO010000000
VERSION     DAZUMO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 145407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 145407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..145407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.001"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(186..1682)
                     /gene="lysS"
                     /locus_tag="ACG8ER_000001"
     CDS             complement(186..1682)
                     /gene="lysS"
                     /locus_tag="ACG8ER_000001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS5048627.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1793..2797)
                     /gene="dusB"
                     /locus_tag="ACG8ER_000002"
     CDS             complement(1793..2797)
                     /gene="dusB"
                     /locus_tag="ACG8ER_000002"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS5048628.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2798..3685)
                     /gene="hslO"
                     /locus_tag="ACG8ER_000003"
     CDS             complement(2798..3685)
                     /gene="hslO"
                     /locus_tag="ACG8ER_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS5048629.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3902..6013)
                     /gene="ftsH"
                     /locus_tag="ACG8ER_000004"
     CDS             complement(3902..6013)
                     /gene="ftsH"
                     /locus_tag="ACG8ER_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS5048630.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6103..6648)
                     /gene="hpt"
                     /locus_tag="ACG8ER_000005"
     CDS             complement(6103..6648)
                     /gene="hpt"
                     /locus_tag="ACG8ER_000005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS5048631.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6648..8093)
                     /gene="tilS"
                     /locus_tag="ACG8ER_000006"
     CDS             complement(6648..8093)
                     /gene="tilS"
                     /locus_tag="ACG8ER_000006"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS5048632.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8213..8683)
                     /locus_tag="ACG8ER_000007"
     CDS             complement(8213..8683)
                     /locus_tag="ACG8ER_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS5048633.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8729..9154)
                     /locus_tag="ACG8ER_000008"
     CDS             complement(8729..9154)
                     /locus_tag="ACG8ER_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS5048634.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9221..9490)
                     /locus_tag="ACG8ER_000009"
     CDS             complement(9221..9490)
                     /locus_tag="ACG8ER_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS5048635.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9501..11096)
                     /locus_tag="ACG8ER_000010"
     CDS             complement(9501..11096)
                     /locus_tag="ACG8ER_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5048636.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11111..14632)
                     /gene="mfd"
                     /locus_tag="ACG8ER_000011"
     CDS             complement(11111..14632)
                     /gene="mfd"
                     /locus_tag="ACG8ER_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS5048637.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVSDQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14649..15209)
                     /gene="pth"
                     /locus_tag="ACG8ER_000012"
     CDS             complement(14649..15209)
                     /gene="pth"
                     /locus_tag="ACG8ER_000012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS5048638.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15562..16536
                     /locus_tag="ACG8ER_000013"
     CDS             15562..16536
                     /locus_tag="ACG8ER_000013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5048639.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16706..18106
                     /locus_tag="ACG8ER_000014"
     CDS             16706..18106
                     /locus_tag="ACG8ER_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS5048640.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18184..18906)
                     /locus_tag="ACG8ER_000015"
     CDS             complement(18184..18906)
                     /locus_tag="ACG8ER_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5048641.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18985..19491)
                     /locus_tag="ACG8ER_000016"
     CDS             complement(18985..19491)
                     /locus_tag="ACG8ER_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5048642.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19614..21413
                     /locus_tag="ACG8ER_000017"
     CDS             19614..21413
                     /locus_tag="ACG8ER_000017"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS5048643.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21481..23055)
                     /locus_tag="ACG8ER_000018"
     CDS             complement(21481..23055)
                     /locus_tag="ACG8ER_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS5048644.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23310..23819)
                     /gene="mreD"
                     /locus_tag="ACG8ER_000019"
     CDS             complement(23310..23819)
                     /gene="mreD"
                     /locus_tag="ACG8ER_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS5048645.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23823..24677)
                     /gene="mreC"
                     /locus_tag="ACG8ER_000020"
     CDS             complement(23823..24677)
                     /gene="mreC"
                     /locus_tag="ACG8ER_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS5048646.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24832..25470)
                     /locus_tag="ACG8ER_000021"
     CDS             complement(24832..25470)
                     /locus_tag="ACG8ER_000021"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS5048647.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25471..26121)
                     /gene="rpe"
                     /locus_tag="ACG8ER_000022"
     CDS             complement(25471..26121)
                     /gene="rpe"
                     /locus_tag="ACG8ER_000022"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5048648.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26134..27033)
                     /gene="rsgA"
                     /locus_tag="ACG8ER_000023"
     CDS             complement(26134..27033)
                     /gene="rsgA"
                     /locus_tag="ACG8ER_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS5048649.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27198..29267)
                     /gene="pknB"
                     /locus_tag="ACG8ER_000024"
     CDS             complement(27198..29267)
                     /gene="pknB"
                     /locus_tag="ACG8ER_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS5048650.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29264..30004)
                     /locus_tag="ACG8ER_000025"
     CDS             complement(29264..30004)
                     /locus_tag="ACG8ER_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS5048651.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30017..31375)
                     /gene="rsmB"
                     /locus_tag="ACG8ER_000026"
     CDS             complement(30017..31375)
                     /gene="rsmB"
                     /locus_tag="ACG8ER_000026"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS5048652.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31375..32313)
                     /gene="fmt"
                     /locus_tag="ACG8ER_000027"
     CDS             complement(31375..32313)
                     /gene="fmt"
                     /locus_tag="ACG8ER_000027"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS5048653.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32306..32797)
                     /gene="def"
                     /locus_tag="ACG8ER_000028"
     CDS             complement(32306..32797)
                     /gene="def"
                     /locus_tag="ACG8ER_000028"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5048654.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32812..35220)
                     /gene="priA"
                     /locus_tag="ACG8ER_000029"
     CDS             complement(32812..35220)
                     /gene="priA"
                     /locus_tag="ACG8ER_000029"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS5048655.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35473..35778)
                     /gene="rpoZ"
                     /locus_tag="ACG8ER_000030"
     CDS             complement(35473..35778)
                     /gene="rpoZ"
                     /locus_tag="ACG8ER_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5048656.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35783..36397)
                     /gene="gmk"
                     /locus_tag="ACG8ER_000031"
     CDS             complement(35783..36397)
                     /gene="gmk"
                     /locus_tag="ACG8ER_000031"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5048657.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36884..37168)
                     /locus_tag="ACG8ER_000032"
     CDS             complement(36884..37168)
                     /locus_tag="ACG8ER_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048658.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37186..38076)
                     /locus_tag="ACG8ER_000033"
     CDS             complement(37186..38076)
                     /locus_tag="ACG8ER_000033"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS5048659.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38096..38314)
                     /locus_tag="ACG8ER_000034"
     CDS             complement(38096..38314)
                     /locus_tag="ACG8ER_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS5048660.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38284..39984)
                     /locus_tag="ACG8ER_000035"
     CDS             complement(38284..39984)
                     /locus_tag="ACG8ER_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS5048661.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40251..41024
                     /locus_tag="ACG8ER_000036"
     CDS             40251..41024
                     /locus_tag="ACG8ER_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048662.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41105..41674)
                     /gene="thiT"
                     /locus_tag="ACG8ER_000037"
     CDS             complement(41105..41674)
                     /gene="thiT"
                     /locus_tag="ACG8ER_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS5048663.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41740..41842)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41848..42819)
                     /locus_tag="ACG8ER_000038"
     CDS             complement(41848..42819)
                     /locus_tag="ACG8ER_000038"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5048664.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42913..44286)
                     /gene="glmU"
                     /locus_tag="ACG8ER_000039"
     CDS             complement(42913..44286)
                     /gene="glmU"
                     /locus_tag="ACG8ER_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS5048665.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44789..45607)
                     /gene="purR"
                     /locus_tag="ACG8ER_000040"
     CDS             complement(44789..45607)
                     /gene="purR"
                     /locus_tag="ACG8ER_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS5048666.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45774..46598)
                     /locus_tag="ACG8ER_000041"
     CDS             complement(45774..46598)
                     /locus_tag="ACG8ER_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS5048667.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46571..47251)
                     /locus_tag="ACG8ER_000042"
     CDS             complement(46571..47251)
                     /locus_tag="ACG8ER_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5048668.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47274..48212)
                     /locus_tag="ACG8ER_000043"
     CDS             complement(47274..48212)
                     /locus_tag="ACG8ER_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5048669.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48457..49308)
                     /gene="ispE"
                     /locus_tag="ACG8ER_000044"
     CDS             complement(48457..49308)
                     /gene="ispE"
                     /locus_tag="ACG8ER_000044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS5048670.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49462..52035)
                     /gene="mprF"
                     /locus_tag="ACG8ER_000045"
     CDS             complement(49462..52035)
                     /gene="mprF"
                     /locus_tag="ACG8ER_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5048671.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52176..52982
                     /gene="proC"
                     /locus_tag="ACG8ER_000046"
     CDS             52176..52982
                     /gene="proC"
                     /locus_tag="ACG8ER_000046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS5048672.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(53061..54617)
                     /gene="rny"
                     /locus_tag="ACG8ER_000047"
     CDS             complement(53061..54617)
                     /gene="rny"
                     /locus_tag="ACG8ER_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS5048673.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(55028..56077)
                     /gene="recA"
                     /locus_tag="ACG8ER_000048"
     CDS             complement(55028..56077)
                     /gene="recA"
                     /locus_tag="ACG8ER_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS5048674.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56186..57427)
                     /locus_tag="ACG8ER_000049"
     CDS             complement(56186..57427)
                     /locus_tag="ACG8ER_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS5048675.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57667..58245)
                     /gene="pgsA"
                     /locus_tag="ACG8ER_000050"
     CDS             complement(57667..58245)
                     /gene="pgsA"
                     /locus_tag="ACG8ER_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS5048676.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58454..59377)
                     /locus_tag="ACG8ER_000051"
     CDS             complement(58454..59377)
                     /locus_tag="ACG8ER_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS5048677.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(60116..61402)
                     /locus_tag="ACG8ER_000052"
     CDS             complement(60116..61402)
                     /locus_tag="ACG8ER_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5048678.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61395..62663)
                     /locus_tag="ACG8ER_000053"
     CDS             complement(61395..62663)
                     /locus_tag="ACG8ER_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5048679.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62915..63376
                     /gene="mscL"
                     /locus_tag="ACG8ER_000054"
     CDS             62915..63376
                     /gene="mscL"
                     /locus_tag="ACG8ER_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS5048680.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63435..63881)
                     /locus_tag="ACG8ER_000055"
     CDS             complement(63435..63881)
                     /locus_tag="ACG8ER_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5048681.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63974..64219)
                     /locus_tag="ACG8ER_000056"
     CDS             complement(63974..64219)
                     /locus_tag="ACG8ER_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS5048682.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64334..64912)
                     /locus_tag="ACG8ER_000057"
     CDS             complement(64334..64912)
                     /locus_tag="ACG8ER_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5048683.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64989..65519)
                     /locus_tag="ACG8ER_000058"
     CDS             complement(64989..65519)
                     /locus_tag="ACG8ER_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS5048684.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65523..66383)
                     /gene="rlmB"
                     /locus_tag="ACG8ER_000059"
     CDS             complement(65523..66383)
                     /gene="rlmB"
                     /locus_tag="ACG8ER_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS5048685.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66376..66771)
                     /locus_tag="ACG8ER_000060"
     CDS             complement(66376..66771)
                     /locus_tag="ACG8ER_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS5048686.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66768..68180)
                     /gene="cysS"
                     /locus_tag="ACG8ER_000061"
     CDS             complement(66768..68180)
                     /gene="cysS"
                     /locus_tag="ACG8ER_000061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS5048687.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68177..68713)
                     /gene="epsC"
                     /locus_tag="ACG8ER_000062"
     CDS             complement(68177..68713)
                     /gene="epsC"
                     /locus_tag="ACG8ER_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS5048688.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(68799..69038)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(69035..70495)
                     /gene="gltX"
                     /locus_tag="ACG8ER_000063"
     CDS             complement(69035..70495)
                     /gene="gltX"
                     /locus_tag="ACG8ER_000063"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS5048689.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70665..71822)
                     /locus_tag="ACG8ER_000064"
     CDS             complement(70665..71822)
                     /locus_tag="ACG8ER_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS5048690.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71856..73226)
                     /gene="radA"
                     /locus_tag="ACG8ER_000065"
     CDS             complement(71856..73226)
                     /gene="radA"
                     /locus_tag="ACG8ER_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS5048691.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73289..73816)
                     /locus_tag="ACG8ER_000066"
     CDS             complement(73289..73816)
                     /locus_tag="ACG8ER_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS5048692.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            74046..74852
                     /gene="proB"
                     /locus_tag="ACG8ER_000067"
     CDS             74046..74852
                     /gene="proB"
                     /locus_tag="ACG8ER_000067"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS5048693.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74849..76096
                     /locus_tag="ACG8ER_000068"
     CDS             74849..76096
                     /locus_tag="ACG8ER_000068"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS5048694.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76172..76516
                     /locus_tag="ACG8ER_000069"
     CDS             76172..76516
                     /locus_tag="ACG8ER_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048695.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76633..76959)
                     /locus_tag="ACG8ER_000070"
     CDS             complement(76633..76959)
                     /locus_tag="ACG8ER_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048696.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77574..78776)
                     /locus_tag="ACG8ER_000071"
     CDS             complement(77574..78776)
                     /locus_tag="ACG8ER_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5048697.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78893..79408
                     /locus_tag="ACG8ER_000072"
     CDS             78893..79408
                     /locus_tag="ACG8ER_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5048698.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79607..80053)
                     /locus_tag="ACG8ER_000073"
     CDS             complement(79607..80053)
                     /locus_tag="ACG8ER_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5048699.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(80100..81578)
                     /gene="msr(C)"
                     /locus_tag="ACG8ER_000074"
     CDS             complement(80100..81578)
                     /gene="msr(C)"
                     /locus_tag="ACG8ER_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5048700.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81932..82561)
                     /locus_tag="ACG8ER_000075"
     CDS             complement(81932..82561)
                     /locus_tag="ACG8ER_000075"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5048701.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82545..83009)
                     /locus_tag="ACG8ER_000076"
     CDS             complement(82545..83009)
                     /locus_tag="ACG8ER_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5048702.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83496..84764)
                     /locus_tag="ACG8ER_000077"
     CDS             complement(83496..84764)
                     /locus_tag="ACG8ER_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5048703.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84995..85732)
                     /locus_tag="ACG8ER_000078"
     CDS             complement(84995..85732)
                     /locus_tag="ACG8ER_000078"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5048704.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85799..86368)
                     /locus_tag="ACG8ER_000079"
     CDS             complement(85799..86368)
                     /locus_tag="ACG8ER_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS5048705.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86496..87368
                     /locus_tag="ACG8ER_000080"
     CDS             86496..87368
                     /locus_tag="ACG8ER_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5048706.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87710..88582
                     /locus_tag="ACG8ER_000081"
     CDS             87710..88582
                     /locus_tag="ACG8ER_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5048707.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88579..90537
                     /gene="nagE"
                     /locus_tag="ACG8ER_000082"
     CDS             88579..90537
                     /gene="nagE"
                     /locus_tag="ACG8ER_000082"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS5048708.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90869..91720)
                     /locus_tag="ACG8ER_000083"
     CDS             complement(90869..91720)
                     /locus_tag="ACG8ER_000083"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5048709.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91843..92310
                     /locus_tag="ACG8ER_000084"
     CDS             91843..92310
                     /locus_tag="ACG8ER_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS5048710.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92573..93055)
                     /locus_tag="ACG8ER_000085"
     CDS             complement(92573..93055)
                     /locus_tag="ACG8ER_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5048711.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(93155..94018)
                     /locus_tag="ACG8ER_000086"
     CDS             complement(93155..94018)
                     /locus_tag="ACG8ER_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5048712.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            94616..95491
                     /locus_tag="ACG8ER_000087"
     CDS             94616..95491
                     /locus_tag="ACG8ER_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS5048713.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(95636..95788)
                     /locus_tag="ACG8ER_000088"
                     /pseudo
     CDS             complement(95636..95788)
                     /locus_tag="ACG8ER_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(96056..97387)
                     /locus_tag="ACG8ER_000089"
     CDS             complement(96056..97387)
                     /locus_tag="ACG8ER_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5048714.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(97536..98249)
                     /locus_tag="ACG8ER_000090"
     CDS             complement(97536..98249)
                     /locus_tag="ACG8ER_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5048715.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            98424..99848
                     /locus_tag="ACG8ER_000091"
     CDS             98424..99848
                     /locus_tag="ACG8ER_000091"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5048716.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            100147..100854
                     /locus_tag="ACG8ER_000092"
     CDS             100147..100854
                     /locus_tag="ACG8ER_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5048717.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            100841..101164
                     /locus_tag="ACG8ER_000093"
     CDS             100841..101164
                     /locus_tag="ACG8ER_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5048718.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(101253..102290)
                     /locus_tag="ACG8ER_000094"
     CDS             complement(101253..102290)
                     /locus_tag="ACG8ER_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5048719.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(102611..103594)
                     /locus_tag="ACG8ER_000095"
     CDS             complement(102611..103594)
                     /locus_tag="ACG8ER_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS5048720.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(104009..104779)
                     /locus_tag="ACG8ER_000096"
     CDS             complement(104009..104779)
                     /locus_tag="ACG8ER_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5048721.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(104772..105707)
                     /locus_tag="ACG8ER_000097"
     CDS             complement(104772..105707)
                     /locus_tag="ACG8ER_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048722.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(106023..107672)
                     /locus_tag="ACG8ER_000098"
     CDS             complement(106023..107672)
                     /locus_tag="ACG8ER_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5048723.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(107789..110392)
                     /locus_tag="ACG8ER_000099"
     CDS             complement(107789..110392)
                     /locus_tag="ACG8ER_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5048724.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(110404..111454)
                     /locus_tag="ACG8ER_000100"
                     /pseudo
     CDS             complement(110404..111454)
                     /locus_tag="ACG8ER_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(111480..111794)
                     /locus_tag="ACG8ER_000101"
     CDS             complement(111480..111794)
                     /locus_tag="ACG8ER_000101"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS5048725.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(111810..112256)
                     /locus_tag="ACG8ER_000102"
     CDS             complement(111810..112256)
                     /locus_tag="ACG8ER_000102"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5048726.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(112257..114179)
                     /locus_tag="ACG8ER_000103"
     CDS             complement(112257..114179)
                     /locus_tag="ACG8ER_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5048727.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(114391..115287)
                     /locus_tag="ACG8ER_000104"
     CDS             complement(114391..115287)
                     /locus_tag="ACG8ER_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5048728.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(115280..115960)
                     /locus_tag="ACG8ER_000105"
     CDS             complement(115280..115960)
                     /locus_tag="ACG8ER_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5048729.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(115961..116743)
                     /locus_tag="ACG8ER_000106"
     CDS             complement(115961..116743)
                     /locus_tag="ACG8ER_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5048730.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(116736..117653)
                     /locus_tag="ACG8ER_000107"
     CDS             complement(116736..117653)
                     /locus_tag="ACG8ER_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5048731.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(117817..118071)
                     /locus_tag="ACG8ER_000108"
     CDS             complement(117817..118071)
                     /locus_tag="ACG8ER_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048732.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            118928..122155
                     /gene="fss3"
                     /locus_tag="ACG8ER_000109"
     CDS             118928..122155
                     /gene="fss3"
                     /locus_tag="ACG8ER_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS5048733.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            122268..122582
                     /locus_tag="ACG8ER_000110"
     CDS             122268..122582
                     /locus_tag="ACG8ER_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5048734.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            122595..122969
                     /locus_tag="ACG8ER_000111"
     CDS             122595..122969
                     /locus_tag="ACG8ER_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5048735.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            122979..123314
                     /locus_tag="ACG8ER_000112"
     CDS             122979..123314
                     /locus_tag="ACG8ER_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS5048736.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            123397..124737
                     /locus_tag="ACG8ER_000113"
     CDS             123397..124737
                     /locus_tag="ACG8ER_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5048737.1"
                     /translation="MYKGHRIRAGDQHLVYHFVIGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            124815..125489
                     /locus_tag="ACG8ER_000114"
     CDS             124815..125489
                     /locus_tag="ACG8ER_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5048738.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            125482..125646
                     /locus_tag="ACG8ER_000115"
     CDS             125482..125646
                     /locus_tag="ACG8ER_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048739.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            125722..126864
                     /gene="dcm"
                     /locus_tag="ACG8ER_000116"
                     /pseudo
     CDS             125722..126864
                     /gene="dcm"
                     /locus_tag="ACG8ER_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            126854..127144
                     /locus_tag="ACG8ER_000117"
     CDS             126854..127144
                     /locus_tag="ACG8ER_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048740.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            127403..128587
                     /gene="mobT"
                     /locus_tag="ACG8ER_000118"
     CDS             127403..128587
                     /gene="mobT"
                     /locus_tag="ACG8ER_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5048741.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            128584..128721
                     /locus_tag="ACG8ER_000119"
     CDS             128584..128721
                     /locus_tag="ACG8ER_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5048742.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            129467..131377
                     /gene="ltrA"
                     /locus_tag="ACG8ER_000120"
     CDS             129467..131377
                     /gene="ltrA"
                     /locus_tag="ACG8ER_000120"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5048743.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            131481..131705
                     /locus_tag="ACG8ER_000121"
     CDS             131481..131705
                     /locus_tag="ACG8ER_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048744.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            131718..132221
                     /locus_tag="ACG8ER_000122"
     CDS             131718..132221
                     /locus_tag="ACG8ER_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5048745.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            132281..132670
                     /locus_tag="ACG8ER_000123"
     CDS             132281..132670
                     /locus_tag="ACG8ER_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5048746.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            132657..135104
                     /locus_tag="ACG8ER_000124"
     CDS             132657..135104
                     /locus_tag="ACG8ER_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5048747.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            135109..137232
                     /locus_tag="ACG8ER_000125"
     CDS             135109..137232
                     /locus_tag="ACG8ER_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5048748.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            137229..138233
                     /locus_tag="ACG8ER_000126"
     CDS             137229..138233
                     /locus_tag="ACG8ER_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5048749.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            138252..139163
                     /locus_tag="ACG8ER_000127"
     CDS             138252..139163
                     /locus_tag="ACG8ER_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5048750.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            139350..139606
                     /locus_tag="ACG8ER_000128"
                     /pseudo
     CDS             139350..139606
                     /locus_tag="ACG8ER_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(139823..140038)
                     /locus_tag="ACG8ER_000129"
     CDS             complement(139823..140038)
                     /locus_tag="ACG8ER_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048751.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(140287..140505)
                     /locus_tag="ACG8ER_000130"
                     /pseudo
     CDS             complement(140287..140505)
                     /locus_tag="ACG8ER_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(140800..142239)
                     /locus_tag="ACG8ER_000131"
     CDS             complement(140800..142239)
                     /locus_tag="ACG8ER_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5048752.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            complement(142827..143459)
                     /locus_tag="ACG8ER_000132"
     CDS             complement(142827..143459)
                     /locus_tag="ACG8ER_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS5048753.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            complement(143808..145259)
                     /locus_tag="ACG8ER_000133"
     CDS             complement(143808..145259)
                     /locus_tag="ACG8ER_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5048754.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT        42484 a        29902 c        26496 g        46525 t
ORIGIN      
        1 ttttttgtat tttttcaagt ttttttcaat aaacgattat tcataacttt tctttcttta
       61 tcgttcgttt tcgtcttctt taatgtctag aatagtcggc attagctttt gaactttcgt
      121 tattatttat tcctttctac acagcaaaag ctgcctaagg aattccttag acagctttta
      181 ttcgattatc tcattgttgg gaatagcaaa acatcccgaa ttgattgtgc atcagttagc
      241 aacattacta atctgtcgat cccgatgcct agaccaccag ttggcggcat accgtattct
      301 aaggcttcca agaagtcttc gtctacaccg tgagcttcgt catttcctag ttcacgttct
      361 ttttcttgcg cttcgaatct ttctctttga tcaattggat cgttcagttc tgtaaaggca
      421 ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat ctgtgaatcg aggatcttct
      481 ggatttttct tcgctaaagg cgaaacttcg actggatgtc cgtatacaaa cgtcggttgt
      541 gtcaatgttt cttcaacaaa tgtttcaaag aattcattga taacatgtcc gacacccata
      601 gcttcggtta cttctacatt gtgtttttcg gctaaagcta atgcttcttc caatgtcatt
      661 tcttgccaga agtctacacc agtttgttct ttgattgcat ctaccatatg gatgcgttta
      721 aagtcacttt ctaagtcgac agcttgtcca tcatatgtga tttttgctgt tcctagtact
      781 ttttcagctg cgttacggat aataccttct gtcagattca ttacatcatt gaaatcagta
      841 tatgctgtgt atgcttctaa catcgtaaat tctggattat gtgtagtatc aattccttca
      901 ttacggaaaa cgcggccgat ttcataaact ttttccattc cgccaacaat tagtcgtttt
      961 aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt ccagtgcatt atgatgcgtg
     1021 ataaatgggc gtgcggcagc tccacctgcc tcattgtgca atactggtgt ttctacttca
     1081 atatagccat tgccatctaa atagcgacgg atttcgctga taatttggct tcgtttcata
     1141 aatcggtcaa agctttcgcg attactgatt aaatccaagt agcgttgtct gtagcgttgt
     1201 tcaatattcg ttaagccatg gtatttatct ggcagtggac gaagtgcttt tgacaaaatc
     1261 gtgatttctg aagctttgat agaaacttcg cctgtgtctg ttttcatgat ttgtccagtt
     1321 acaccaaaga aatctccaag gtctgcatgt ttgaatactt cataggcctc atcacctact
     1381 tggtcttttc ttacataaat ttgaatttgg ccttcacgat cttgtagatg cgcaaatcca
     1441 gctttccctt ttcctcggcg agtcatcata cgaccagcta cactagcggt caatcccatt
     1501 tctgctagtt cttcttttgt tcttggatca aatagttcat gcaattcttc tgagttgtgc
     1561 gtgcgttcaa atcgttttcc aaatggatcg attccatctt cacgcaattg ttccattttt
     1621 tgacgacgta caagcatttg atcatttaaa tcttctactt gatgttgttg ttcctgtgtc
     1681 acaaaagttc ctccattctc atgttaatct ctttcctata atgccaatga aacacaaaaa
     1741 aagcaagccg aacttggaat ttcggcttga aaattcacga aaaacttaga aatcacaacg
     1801 tttgtttcaa tagtactttt tccgcttttt ctacaaattg atccagcagg tcattgattt
     1861 cttgctgttt ttctgtctga ttgattgcta ctttcacttt cgctgctcga ggaatccctt
     1921 ttaaataata agccgcatgt gttctgaatt ctctacaagc tactttttct cctttgagat
     1981 ctgctaagcg ttgtaaatga agtttagctg tagcaatttt ttcggctggt gttggttcag
     2041 gaatcagttc ccctgtttcg agataatgct gtgtgcggtg aatcatccat ggattgccca
     2101 gcgctgcacg accaatcatc acaccgtctg caccgacata gtcaagcatc cattttgcat
     2161 cttctggcgt tttgacatcc ccgttcccca taaatggaat cgtcaaatgt tttttcactt
     2221 ctttcaatac ttcccagtcc gcttttcctt catacatctg aacccgtgta cgtccatgca
     2281 tagcgatggc acttgctcca gctgattgtg ctgctaatgc attttctaca gcaaagacgt
     2341 gctcttcatc ccaaccaata cgcatcttga ttgttactgg gatatttact gctgaagaaa
     2401 cagcttggac catttcgtat actttctcag gatctaaaag ccattttgct cctgcttctg
     2461 ctttgattac tttatttact ggacagccca tattgatatc tattatatcg gcagctgtgt
     2521 tttcttcgac aaattgagca gcttcaacta gactatcttt atttccgcca aatatctgta
     2581 aacttaatgg atgttctgtt tcttcaatgt ggagcatctc taaagttttt ttgtttctga
     2641 aatgaatacc ttggtcactg atcatttcac atacgactaa gcccgcacca aattctttga
     2701 cagtgactcg aaatgctgaa tttgttattc cagccatcgg tgcaacaacg acacgattag
     2761 ggatttccac attgccaatt ttccatgtgt gttccactta aatccctcct aaggcttctt
     2821 tcttcaatcc atctaattct tcttcgctat acttgtattt gttattacag aaagaacaaa
     2881 ccgcttctgc accgtgatct tcgtctatca ttgcttggat ttcttctgca cctaacgcga
     2941 taattgcttc tgcaaatttt tcttttgaac agtcacattt gaattgaaca ggcatttttt
     3001 ccaaaatgtt caattcttct gtaccaagca atcctttcaa tatttcttct ggtgtctgtc
     3061 cttcatctaa gagggtagag attcttggtg tcgtttttaa ttgttcttcg atcttgctga
     3121 ttgtttcttc atcggcacct ggcatgattt ggatcatgaa tcctcccgcc gtcttgatgc
     3181 tgtcgtcagt attgaccaat acactcagtc caacagctga agggatttgt tctgagatag
     3241 caagatagta agtaaaatct tcagcgattt ctccagaaac aatcggcgtt tgcccagaaa
     3301 aaggttcttt taatcctaag tcttttgtga cagtaaacat tccttgtgtt ccaactgctc
     3361 cccgcacatc gattttacct tcactattta gaggaagact gagatgcgga tttttgatgt
     3421 aacctttcac atctcccttt ccatttgcat ctactacaat tcctgttgct ggaccatcgc
     3481 cttggattcg aacagttagt ttgtcttctc ctttgagcgt cgcaccaagt agcagactac
     3541 cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca agtatcatga cgtctttgtg
     3601 cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg gacgaatccg tcgtgagcaa
     3661 gtgccttaac caaataatct ttcatttgtt atgtactcct ttttccgttt ttaaactcat
     3721 agtgaatcat actagcaact actgcttttt tcaactttgg aatgcttctt ttaaaaataa
     3781 ttcagaatca tctagaaaac aggacatcac cctatttaca acttaaataa agaaatctcc
     3841 cgaaaaccgg tttttgttcc ggttttcggg agtcatatca gatcaattga cctatatttt
     3901 tttatttgta ttgatcgtct gaatgattat cttctgattg ttgagaatct gaatcatgtt
     3961 ctgctttatt ttcctcattc agttcatgtt ttgcttcttc aaaatcacgt ttttcttccg
     4021 cttgtttttc agcgtctttt tcttccaaag cacgttttgc ttcttcaaaa gttttcgctt
     4081 ctttttcgct tggataatca tttgactcag taccttcagg catcacaccg tgttcaaaca
     4141 atgacttgat tgctttggca tccaatgttt cgaattccaa aagtttttca gcaatcagtt
     4201 tatgctgtgc acgatgagct tcgatgattt ctcgagcttt atcatgagct tccatcaaga
     4261 tttttcgtac ttcttgatca atctcgaaag cgacttgttc cgagtaagct tttgtttgac
     4321 catagtcgcg gccaacaaac acttgatggt ttccttcata ttgaacagga cctaaacgat
     4381 cactcattcc atattctgtt accatgcttc gtgctagagc tgtcgcttgc tcaaagtcat
     4441 tggaagcacc agttgattgg acattgaaga tgatttcttc agctgtacgt ccaccaagta
     4501 agcctacgat ttgttcaaac atatcttctt tcgtcatcaa gaactgatct tcttttggta
     4561 aagcgatcat atatccgccg gcgcgtccgc gagggatgat cgttactttg tggacgaccc
     4621 gagcgcgact caatactaat ccgacaatcg tatgaccagc ttcgtgataa gcaaccattt
     4681 cgcgttcttt tttgctgatt acgcgatctt ttttagctgg acctgcaatc acgcgatctt
     4741 ccgcttcatc gatatcagaa gcatcgattt tctttttatt tcttctagca gctactaacg
     4801 ctgcttcatt caagacattt tctaaatctg ctccggcaaa acctggtgtc tgttgagcca
     4861 caactttcaa gtcaacgtca tctgccatcg gtttattccg tgcatggact cgcaagattg
     4921 cttcacgacc ttttacatct ggacgtccaa ctaaaatctg gcggtcaaaa cggcctggac
     4981 gtagtaacgc tgggtctagt acatcagaac ggttggttgc agcaatgacg attactcctt
     5041 catttccgtc gaagccatcc atttctacaa gcaattggtt caatgtttgt tcgcgttcat
     5101 catgtccgcc gcccataccg gcaccacgtt gacgacctac cgcatcaatt tcatcaatga
     5161 aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa atcacgaaca cgacttgctc
     5221 caacaccgac aaacatttct acgaaatcag aacccgagat cgagtaaaac ggtactccag
     5281 cttctccggc aacagctttt gctaataagg ttttacctgt tcctggaggt ccttctaaaa
     5341 gtacacctgc cgggatacgg gcacctaatt caacaaatcg gcgaggatct ttcagaaatt
     5401 caacaacttc aactagttct tgtttttctt cttccgcacc cgctacatca gaaaaacgta
     5461 cgcgattagc ttttttgtct gcttctttgg cttttgattt accaaagttc atgacacggc
     5521 cgccaccgcc accgccgccg ccttgttgtc ccatcatcat gtaaaagaaa aagatgatga
     5581 ttacgattgg caagaagcta agcaataggg aaacccatat accgctgctt gattcttctt
     5641 ttactgttgt agcaactttg ttttcttttg ccagttctgt tacttggctc aatgttgtat
     5701 catttggcaa aatgattgtt gtgaaatggg tagtagtcgt atctgttgaa cctaagatag
     5761 ctaacccacc gctgttagcg acggtctgct gttctttgta ttcaccagtg atcttatata
     5821 caccatttgc aggctgcact tcaaattctt tgatcttgcc tgcttgtagt tgttctgtga
     5881 attttgagta ctcgatatct gacgatcgct ggccgttatt accaaaaatg aaataaacga
     5941 ccattaccat cgctaagatt acgaggacat aatacaaggc atttttcatc atgccgttgt
     6001 tctttttgtt catagttgcc ctccctaagc gtcaatttaa tttaacagtt ttttatcaat
     6061 cctgaccatt ctattgtacc ataatttaat tgagatacag cattaatttg attgataaac
     6121 actaggtttc aatacaccga tgtacggaag gttacgatat gcttctgcat aatccaagcc
     6181 ataaccgacc acaaattcat ttggtacgtt gaagccaaca taatctgctt cgatatttac
     6241 tactcttcct tcaggtttgt caagtagtgt aacaattttg actgattttg ctttgcggta
     6301 tttgaaaaga tccactaaat aagcaagtgt tcttccgctg tcaatgatat cttcaacgat
     6361 caacagatcg cgaccttcta cattcgtatc taagtctttg acgattttta cttcccctga
     6421 cgagactgtt gcattcccgt agcttgaaac atccatgaaa tccaattcca aatacgtatc
     6481 gatagaacga acgatatctg ccatgaatgg taccgctcct tttaagacac ctactactag
     6541 gggattggtg ttttggtaaa tttctgttaa ctctttgcct agttctttgc aacgaacttg
     6601 tatctcttct tgtgagatca aaattctctc aatatccttt tccaacatca ggttctcctt
     6661 ccaattgctt ctttttgata tttgtaaaga agtatgtagt gtattttatc agtttcttca
     6721 cgaatactca aatatgaaaa ggcaaaaggt atcaagctat atatatttct tttggcatct
     6781 gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt tctgatcgat aaaatatcta
     6841 gatacttttt ttgtgagatt ctctgttaat ttaatgcggt ctcctgcttt tcttttttcg
     6901 acatacagtt gcacatcagc aggaatccaa atgtcatgag aaaatagaga ccatgaatct
     6961 gttgctttga tgtcccctgc attttcaggt ttgaccagac caatccattc tgtttcattc
     7021 aaatatattc cttgttctgg aatcagcaac aagcgctggt cttctgtctg gctagttgct
     7081 tgtttttctt ttttagagag tacaacaaaa tgatactctc ttttcagtat ccaattattg
     7141 ccaatactga gttgccattg gctttttttc tgttgccact ggtctaaaat cgactccatt
     7201 tgttgttctt tcaaaataat tcctgttttt gagaatgctt tgtcgaaaaa ggagtaaaaa
     7261 aaatagtatc gttcagcatc tgtcatttct tcaatcattt ctgcagaaaa tcgaaattgt
     7321 tcctcccact gttcgacttc cttttcgatc aactgtccca tatatttttg aatgacttgg
     7381 tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc tcaacgcctg tgtattttct
     7441 tgtttcaatt gaggaactac caaatgacga agccgatttc tctggatatc taacaattgg
     7501 tttgtctgat cctcaaagta aacaagctgc gattccgctg cataagtata taggtcttct
     7561 ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag caaatggttg tacttctttg
     7621 attccagaaa aagtatttaa ctgtccccca cgaacgattt tcattaacac cgtctcgatt
     7681 tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt gtccttcttc catcacgcga
     7741 gtgaattctt gataacgaaa gttacgagct tctgtttcta tggcggattt ggctggatct
     7801 tcccaaatac ttacatataa agggagattc ttctcttcac aatatgagcg aagataggca
     7861 gcttcttctg ctgattcttc tcttaattgg tgattgatgt ggacgatacc taaagaaaaa
     7921 ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa ccatcgaatc aacaccacct
     7981 gataccgctg ctaaaattct cgatccttgt tgccagaatc cttgttcatt cccgtgccga
     8041 ataaatgact gctgtaatag cggtgctttt ttcccgcctt tttcaacgtt catcctttcc
     8101 acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc taaaacgaca attaacctgc
     8161 cctctgttga tactatgacc caaatagtcg cactatcatt atttgcggct ttttagctgc
     8221 gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg cttcaatgaa gataagcggt
     8281 catcgctatc tttcaaaaat gaactcatca atgaatcgaa gtcttctttt tgagctgaag
     8341 gttgactgcg agaaaaagat tttttctgtg ctggacgatt tcggttgtca cggggatctt
     8401 tttgttgata ttttttagga cggctttctt ctccagattc agcaggttgt tcctgcgctt
     8461 tccggataga tagtccgatt tttccatcat catttactga aataacttta actgttactt
     8521 catctcctac acttaaaaca tcatggatgt cttttacata gccatttgac acttcgctaa
     8581 tatgaactag acccgttttt ccagcaccta gatcaataaa cgcaccaaaa tttgtgattc
     8641 ctgatacttt tccttgcagt tttgctccta cctcgattga cataaaaaaa atgttcctcc
     8701 taattatttc ccttttttat attcctgctt attttgcagt tgtagaacta gttgattgat
     8761 ctgtttcatt tgtcgagctg tctgtatttg attctaatat aggataaact ttttcaccgt
     8821 cttttgagta aaagaatttg cttctggcaa gtttagctac atactcatca tctttcagca
     8881 atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac ttctttcatt tcagtatttg
     8941 cttcgactct caattcgttc aattgatgca tcctttgcat gtcatggaaa atctgcacac
     9001 ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc agccagtctg cgtcttcgaa
     9061 aaatcagttg acgttgttgt tgctgaaatt cagcatactt tttctttgca tattccgtat
     9121 ctagcgcagc gattttattg ctttttttcg tcacttggat tcgctccaat ctttttgatt
     9181 gtctttttca ttataccaat atcgtgaaag cttgtctaat ctatttctct agtgtttctg
     9241 tgcgattttc agaaataatt tcgtacattt tttgtgcatc ttcttttttg gttgattcat
     9301 gtaattccgt aattttcact tccaacgttt tgttgccaaa ctggactttc aaaatatcgc
     9361 ctactttgac atcactggaa gatttcgcca atttaccgtt gatttgaatt cttcctttat
     9421 cagctacttc ttttgctact gatcgtcttt tgatgattcg ggaaattttt aaaaatttat
     9481 ctaatcgcat atcttattcc tcacttttta atttttttat ttaatcgcag gattttctta
     9541 ccaaaaggca acattagcca ttctcgtact gtaaacagac gaagacgaat cactgcataa
     9601 atgaacacag aagccccgat cccaactcct atcacgctga caaaaaaagc agtggaacga
     9661 tgagttacag gaccaaatac tatattcaat acaccataat acagcagcag tactactgtc
     9721 atacaaagaa ggctgaacat caacttccgt ccaaaatgcc gttctttcca aaaagcattt
     9781 agctcttttg tttcaattcg aacaaaatac caaagcgtca cacctaatcc taaaagggta
     9841 gacaggcttg cacctgctgt tcctaataaa ccggtcagaa aagaagtagt caatcctttt
     9901 accaatagtc cccatcctgc gccttttaaa gaagggcgaa acgagttctt gctttgagca
     9961 atactttgat aagcttggat gactgcagtg aaagcaatgg aaaaaacaaa taatacaagc
    10021 gctgcatttc ccgcataatc tttaaacaac gcataattga tataagggag cagcaaggct
    10081 aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc taagatagat tttggctgtc
    10141 tgtaaaaact gacgatagat ccggttggtc aaatgacgag tcaacgcagg caggaatgtc
    10201 gcacttaatg cagtggcaat cactaaacct aattgtacta aaggctgacc tcgatcatag
    10261 accccttttg ctatcttcgc accgtattct gacagtccgt aaacttccaa agcatttttt
    10321 acaaagaaag agtcgatcag ctggaaaaag ataagcaatc cactataaat cgaaaccagt
    10381 ccgccttcta ccagaaaacg ccggatcaag ggctttgacg gctgtttgaa caatggaaac
    10441 cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat agtagtgaag gatcccatat
    10501 gcgcataaac cgccaaaaac agctccactc attgctgccg tccctgtttg gtacaccgtc
    10561 catccaagct ttctaaaagc aaacgcacta cagacaatca caaaaacacg aacgaactgc
    10621 tcaactactt gagaaacagc agttggttcc atcaagaatc ttccttggaa attccctcga
    10681 taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa ctacttggat cagcggtttc
    10741 aattgtatgt ctcccatcat ccaagcaatc cagctgctaa atgcaaagac aaacaaccac
    10801 aaaaagacac ctgtccaaaa tactaaggga taaagatttt gcaaagcttg ctttcgctcg
    10861 aaaggattct ttctttctgc aacgtacttt gatatgaact gcggaagacc ggataaagct
    10921 aaagtcattg ctatcccata aattggataa acttgctgat aaacataaaa accttcgtcg
    10981 ccgactaagt tttggagggg aattcggtaa agggcactta atacttttgc tatgaaagaa
    11041 gcaatcgtca atacaaatgc tccttgcatg acgcgccgca tttctttatg atccaaatca
    11101 ttgctccttc ctacgctgac agatattttt gctcacgcaa cgctttgaca aacttttgga
    11161 tttcctgaag ccatactgct tcttccattt ggttggggat cgtcaagcgt atcaccatct
    11221 gttctttttc aacgcctaga ctggctttta gttttgtcgc tgtcagtgct tcgaataact
    11281 gttccacact atagcgtttt gttccaactt tacttagagt aaagactata tgcgcctcct
    11341 gtttccttat cgtttctagg agcgctcgat cgccatccat tttgatctgc ccaattgtca
    11401 agagatgtgc tacttcttct ggatattctc caaaacggtc gagcagatca tcttctagtt
    11461 catctagcat ttcttgcgaa tcaagttcac gaattcgttt atagatctcg attttctgcc
    11521 gttgatctga cacgtatgtt tcaggtagat acgcatcgac accgaggtcg atctcaacag
    11581 tggtcttctc cacttgacta ttctttcctt gtttacgaga aacagcttcg cttagcattt
    11641 gagaatacat atcgaaaccg actgcatcaa taaatccatg ctgctgagca cctaagagat
    11701 tacctgcacc gcgaatggaa agatcccgca tcgcaatctt gaatccagat cccagttctg
    11761 taaaatcttt gattgcctga agacgttttt cactgacttc gttaaggatc ttctgctgct
    11821 catacataaa gtaagcatat gcgacacggc tgcttcttcc tacccgccct cgcaattggt
    11881 ataacgtgga taatcccata taatcagcgt tctcaacgaa caacgtattt gcgttcggga
    11941 tatctactcc tgtttcaatg atggttgtag tgacaagaac atcatattga ccttcaataa
    12001 attcaaaaag cgtattttct aattgcgcct ctgtcatttg cccatgagca taaccgatcc
    12061 gtgcttccgg aacgagttct tggatttctt ctactttttg ttcgatcgtt tctacccgat
    12121 tatataaata gaatacttgt ccacctcgtc ccatttctcg atggattgct tcgcgaatag
    12181 ccccaggatt tttttccata acatacgttt ggatcggata acgattcgca ggcggtgttt
    12241 cgattactga aagatcccgc acgcctaaca tcgacatatg caaagttctc ggaatcggtg
    12301 ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg tttcaatcgt tccttatgtt
    12361 ttacaccaaa acgttgttct tcgtcaatga tcagtagacc caaatcgcta aattcaatat
    12421 cttttgacaa aattcggtgg gttcctacaa caatatcaac ttgacctgta cgcagttgtt
    12481 cgatggtttc tttttgttgc tttcttgttc tgaaacgact taatagcccg atatttacgg
    12541 ggaaaccttc aaatctttcc agcatcgttt catagtgctg ctgtgccaag atcgttgttg
    12601 gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt aaaggccgct cgaagcgcta
    12661 cttctgtttt cccataacca acatcaccta cgagtagtcg atccattggt ttttcttttt
    12721 ccatatcccg tttgatctcc gctgcacttc tcaactggtc atcagtttca gagtagggga
    12781 aggcattttc gaattctttt tggtaggcgt catcaggtcc aaaagcatat cctttttctg
    12841 actctcttgc ggcatataat ttgattagat cgtcagcgat atcctcgatt tttgaagaga
    12901 cttttcgctt ggttttcgtc cattcacttc cgcctagctt attgatgcga ggcgctttgg
    12961 cttcagaggc gacatatttt tggatcagat tcagttgagt aacaggaata aacagtttgt
    13021 cgtcgttttg atacaaaata gtcatatagt cttggtgaac gccatctact tctagcgttt
    13081 ccatcccaat gtatttcccg ataccatgat tggcatggac aacgtaatct cctgctttta
    13141 actcattata gcttttcaat cgctctgcgt ttgttactgt ttgacgtctc gtttgttttt
    13201 tcgtcgtttt ttggaaaatt tctttttctg tgatagctac tattttttct tgaggaagtt
    13261 caaaaccagc ctgcaaagct ccttcaacta attgtacctg tcctctgatc aatgcatttt
    13321 tttctgtttc tactactaat atgtcttcat ctcgaagcat ttgttctgct ttgcgtattc
    13381 tgtcctttgt cggaataaag atcacaacag tctgtttttg cttgcgccaa cggtccattt
    13441 ctgttttcag caatggcatt tgtccgaaga actgttgcat cgtccgatat tgaaaattat
    13501 ggattgcttg gaaacgaaga tttcccatcc ctttttggaa gagtgagaaa aaggttgtgg
    13561 tgaacttttc cttctgcacg attttttgta tatctgctga aaatgtctgc tcagaaaaca
    13621 cccgcaattc gctgattttt tgagtctgcc attctgccgc ttccctttcg atttcccgat
    13681 ttgtttccat gattcgctga taatcatcaa cgactagtaa actatttgaa gggaaataat
    13741 ccaagatcgt cacagattct tggtaaagaa gatctgagta gaattttgct tcttctgtcg
    13801 ggacacctgc ttgccagctt gaagtcagct gaccgaagta atcttgcaaa aactcgcggt
    13861 ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat tttttgcatt tgctctattc
    13921 catgttctag atcctctggt ccgtagactt gttcactcgt tggggaaacc cagatttctt
    13981 ccgttttttc gattgagcgt tgtgtttctg cttcaaaata ccgcatagaa tctatctcta
    14041 catcaaatag ttctacacga acaggatagg ttgtcgttaa cgggtaaata tcgataatgc
    14101 ttcctcgaat actgaattct cccggttttg cgaccatctc ttctcgtaca tagcccatca
    14161 aagtcaattg gcgaggaaga ttttccacgt ctatctcatc accaatatgc caattcaatt
    14221 gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg caaagatgcg actggcaaca
    14281 aatagatacc tgcttttttt tcttgaagtg cgttaagcgt tgcaactctc tcgcctcgcg
    14341 cttctggaga agaaaaggcc atttcagcag aaagcacttc atccactgga aataaatgga
    14401 tctgatcttg cggaatcaca tgttgcagat cttctgctaa ctgattcgca taatacaaat
    14461 ttggcatcag aataatcatg tttttatttg ttttcttaaa cgctcgagca aatagaagtg
    14521 ttttcgctga gcccgctaat cctgtgatta gttgtctagg cattttttct tctaaattgc
    14581 tgacccattc ttttacttgc ggatcttttc cgactaattg aataatatcc attccttttc
    14641 ctccttcctc aattgaatcg attcatcgca tccacgaacg aatgcccttc tatgagatat
    14701 tcgactgctt taacagattg acacatgctt tcttctatca aaggctgatt ttctttgctg
    14761 aaaggactaa gcacatgctg gactactgtt tttttccctt ctggcctgcc gattcccacc
    14821 ttgattcgat caaagacatt cgtattcaaa tgggaaataa tacttttgat accgttatgc
    14881 ccgccggcac ttcccttttg acggagccgg atttttccca ctgccagatc caaatcatcg
    14941 tagattacga ctaattcctc tggataaatc ccaaaataag tcattagtgg gccgactgca
    15001 cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa ggatcttttc tccattatga
    15061 aaaaattcgg caacttctgc ttcgaacgga ttttttttga aagtagcttg atgctcgcgt
    15121 gcaattttat ccacgaccat aaagccgata ttgtgttttg tatattggta tttggtacct
    15181 gggtttccta acccgacgat cattttcatt tttgttttcc acctaattct aaaaagcagc
    15241 acaaaaggct ggactgctaa ttagcggtcc agtccatcga gcttttttct tttttacact
    15301 tgtcaatgca tagtataaca caaaacaagg cgtttcggct aaaagagaat cgttaataaa
    15361 tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat aattgatata taaaaatagt
    15421 gaaataacgc acatgtaagc ttttacatat aatacaacct tgttatctgt tatattttat
    15481 agccaaatag cgtgacatgg ggcttgagaa atgttagcat aagcatggga aaaaacaaat
    15541 caatggaagg attgatacta aatgactgca gctgcagaaa aaaaagatca tcaaaaagtt
    15601 attcttgttg gagacggcgc cgtaggttct agctatgcct ttgctctagt tactcaaaat
    15661 atcgctcaag aagtaggtat tgtggatatc aatactgcta aaactgaagg agacgcaatt
    15721 gacttgtctc acgctttggc atttacttca cctaaaaaaa tctattctgc tacttacgca
    15781 gacgcacatg atgctgactt agttgttatt acagctggcg caccacaaaa accaggtgaa
    15841 actcgtttag acttagtaaa caaaaaccta aaaatcaacc gcgatgttgt cacacaaatc
    15901 gttgaatcag gatttaacgg tatcttttta gttgccgcta acccagtaga tattttgact
    15961 tactcaactt ggaaattctc aggtttccct aaagaacgcg taatcggttc aggtacctct
    16021 cttgactcag ctcgtttccg tcaagcactt gcggaattag ttgacgttga tgcacgtaac
    16081 gtccatgctt acatcttagg tgaacacgga gattcagaat tcccagtttg gtcacatgca
    16141 aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca acccagaaat cgatgaagaa
    16201 gctatggtta acctattctt tggcgtacgt gacgctgctt acacaattat cgagaaaaaa
    16261 ggcgctactt tctatggtat cgcagtagca ttagcacgta tcactcgtgc catcttagat
    16321 gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg gcgaatatgg attgaacgac
    16381 atctacattg gtgcacctgc tgttatcaac cgtcaaggta tccaaaaagt aatcgaaatt
    16441 cctttaagtg acagcgagca agaccgtatg gctgcttctg ctaagcaatt aaaagaaatt
    16501 ctagatgaag cttttgctaa actagacgct gaataatctt tgaacatgac ggaaaacatc
    16561 tattcggtgt tttccgtttt tttattttat cacccttatg gtttctaaat tttttcataa
    16621 agtagagata accttcgtct tttcctctaa attatgctat catgagaccg ttccacccta
    16681 aacttttact taaggagttg tcgatatgac aagatcttcg catcgaaaaa aatcgaaaca
    16741 tgttgctttg ctttcagttc ttggtgttct cttagtgctg atcagctttt atacctatcg
    16801 cagtacatac tacacaaagc attttttgcc gaatacagaa ataaatggaa taaatgtcag
    16861 caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa gcttattctg ataaaaaact
    16921 ggctattaaa gaagatggaa aagtctggca ggaagtacca aaatccgagc tcggctacaa
    16981 agatgatttc acttcggagc tttctccgat cctaaatgaa caaaacggat ggacttgggg
    17041 aatgacttat gtctcagcgg cagaaaaaca agaaatagat cctgtcagtc aaaaccacca
    17101 aaaactagac actaccgttc agacattgac aactaagctg actgatttga acaaagaccg
    17161 gacacctact actgatgcaa cgatcgaaaa agcaggtgac tctttcgtta tcaagcctga
    17221 agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa caattaaaaa gtgcggtaaa
    17281 ctctggtaaa gatacaattg aattgactga attcaaagaa aaaccaaaag taacatctga
    17341 agacagctca ctgaaagaac aactcgcatc catgaatgct atcgcaaatg taaaagctac
    17401 ttacagtata aacggtgaga ctttccagat cccatcaagt gatattatga gttggctgac
    17461 ctacaatgat ggaaaagtcg atttagatac agaacaagta cgtcaatatg taacagattt
    17521 aggcacaaag tataacacaa gtacgaatga cacaaaattt aaaagtacaa aaagaggaga
    17581 agttacggtc ccagttggca cttacagttg gacgatccaa acagacagtg agacagaagc
    17641 attgaaaaaa gcgattctgg caggccaaga ttttactcgt tcacccattg tgcaaggtgg
    17701 cacaaccgct gatcatccat tgatcgaaga tacgtatatc gaagttgatc tggagaacca
    17761 gcatatgtgg tattacaaag acggaaaagt ggcccttgaa acagatatcg tttccggaaa
    17821 gccaactaca cctacacctg ctggagtctt ctatgtttgg aataaagaag aagatgcaac
    17881 tttaaaagga acaaatgacg atggaacacc ttacgagagt ccggtaaact actggatgcc
    17941 gattgactgg acaggtgttg gtatccacga ttcagactgg caacctgaat acggtggaga
    18001 cttatggaaa acccgcggtt cacacggctg catcaacacc ccaccaagtg taatgaagga
    18061 attattcggg atggtcgaaa aaggaacgcc tgtattggtc ttctaacaaa aataatatat
    18121 aaaaagaaag cacccgtacg attggtacag gtgctttctt tttattatct aacaatgtgc
    18181 tacttacatt tcagcagcag taaagacttc tgaaacatca tcatcgtctt ctagcttatc
    18241 tactagacgt tccaattgct ctttttgttc ctcatttaaa tcaatcgtcg tttgtggaat
    18301 catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc aactcatctc gtacagcagc
    18361 aaaatcttct ggtgcagtat aaatctcgaa tacttcagga gaagtttgca gatcttctgc
    18421 ccccgcctct aatacatcct caaacatcgt ctcttcatca acagtcaacc catcgcgttc
    18481 aatagcgata tatcctttgc ggtcaaacaa gtaactgact gaacccgttt ctcccagtga
    18541 tccaccgttt cgagtaaaag ctactcggac atttgtcgct gtccgattac ggttatctgt
    18601 caatgcatgg actaagattc ctactccacc cggaccatat ccttcatacg tgatttcatc
    18661 gtaattcgtt tcatctacgg cactcgtagc ctttttaatc gcccgttcca cattgtcgtt
    18721 aggcatattt gcagcttttg ctttgtccac aacaagccgc aaagctggat tcatagatgg
    18781 atcaggaccg cctgctttag ctgccatata aatttcacga gaaagtttct gaaagacttt
    18841 ccctcgtttt gcatcttggg cattttttct tccttggata ttatgccatt tactatgtcc
    18901 tgacatgatt tttcctccct tgttcttttg agaatacact tctattctag tactatttca
    18961 caaaaaaaca aagccacagc caatctataa acgcttcttg aatgccgcaa aaagcacaca
    19021 taatatcaca gcagtcagag caccgatcga atccaacatg acatcttgga acataggtgt
    19081 tcgtccaccg gtcatcatct gatggaattc gtccaaacct gcgtaccctg ttgctgaaag
    19141 ccaagcaagg atcgctgtca gccaccaaat ttttagcttg gggaaaacac ctaaaaacaa
    19201 gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc ttccttaaaa agaactctaa
    19261 aaatttggcg tagcccagat ggttgatact caccatactt ccaccataat taaaacgtat
    19321 acccgctaat gtgtctttaa atggctgatt aggcagccag ctttctaatc gcgaaacttg
    19381 tgattgctgt ccgtaagttt gagaagaact gtaaaataag attataacga ctatcagtgc
    19441 gattactaaa taaatatttc cgtttttcat ttgttttttt gaagaattca taagtcacct
    19501 ctgtatttta tcttagactt tttttgaaga aacaacaatc attttcatag agacttcttc
    19561 cataaaaaag tgctatactt aattttgtat tacaatatga agggagtttt ttcatgagtt
    19621 attcattaaa ttggaatcta gattctattt tttccggagg cagtcattct gatgctttga
    19681 accaacggat gaagcaattg gaagaccaaa tgaacgagta ttatcaccgc gtaacaaaat
    19741 ggtcacctag ttctgacaat gcagagcagt taaacgctat tcttcaattg caagaaacga
    19801 ttacgaatgg ttttacccaa tgcagcagct atattactgc attgctttct gccaatgtca
    19861 atgattcaga tgcaaaggtc ttatcaggca agctttacgc aatgctgcct cgtctgcaat
    19921 cagccgaaac tgtattgtca aagaaatttg cagaaatatc tgacaatgat tggaatcaga
    19981 tgctttctca atcttcgttt gaaacaattg cttttcgttt aaacgagatt cgtcgtgatg
    20041 gctcacaatt attatcagaa gctgaagaaa acattataaa tacgctttct ttagatggtc
    20101 taaatgcttg gagcagtcat tatgatacga ttgtagccag catttcgatt cccttcgaac
    20161 aagatggaca agtagtcgaa ttatctgctg gacaagcctt caataagatg atgggcgatc
    20221 cagatccaaa agtcagagaa acacttttcg cagcatggga agaagcctgg aaagaaaagg
    20281 cacccctctt cacggatact ttgaatcatt tagatggttt tagactttct gactatgaac
    20341 ttcatggtgt cacagatttt ctacaaaaac cattagagta caatcggttg aagaaagaaa
    20401 cattgtctgt catgtgggat acaatccaga aaaacaaaca accccttgtt aattacttga
    20461 ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg gcaagatcaa gatgcaccta
    20521 ttattttagg cgatttgaaa gaaaaaacct ttagttttga cgaagcagca gcatttatta
    20581 tcgaaaactt taacaaattc agtccaaaaa tggctaaatt tgcccaatct gcttttgaaa
    20641 agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc tggtggttat tgtactgagt
    20701 taccagaaac aaaagaatca aggatcttta tgacctacag taattctgtt aatgaggttg
    20761 cgacacttgc tcatgaacta ggacatgctt tccatagcag cacgatgtgg gatttacctt
    20821 ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc aagtacattc gcagaactga
    20881 tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga agaaaaaatc aatttgctag
    20941 atacaaaact gcaaaatgct ttagctatgt tcatgaatat ccattcccga ttcatttttg
    21001 aaaatcgatt ctatgaagca cggcaagaag gattagtctc agaagataag atcacagaga
    21061 tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt agctacttac catccatatt
    21121 tctgggctgc aaaattgcat ttctttattg atgacgtacc gttttataac ttcccataca
    21181 catttggcta cctctttagt ttgggcatct atgcttacgc caaccaaaag ggagcaaatt
    21241 ttgaagaaga atatatccat ttactacgcg atactgcctc aatgacgacg gaagatcttg
    21301 cgaagaaaca tttgaatgtc gatcttaccc aaccagattt ttggcaagca ggtatcgaca
    21361 tggtgactga agatatccaa acgttcatgg aattgacaga agaatttatc tgatttctat
    21421 tttatcacaa taaaaaaaga gttagtccag aatgggctaa ctctttttga ttaatcattc
    21481 ttacatgctg acagcaaagt caggggcaaa ccattgaact gaaccaactt ttacgctttc
    21541 gcctggttga ggagcatgga tatattgtcc tccgcctaaa gcaatcgcta cgtggtaagt
    21601 tccgccttgt gacccccaga ataacaagtc tccagctttt gcttgagaaa ctgaaatttt
    21661 tgttccagct gattcttgag gaactgtcca accaccgatg tcacgaccag ttacttgcat
    21721 gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt ggatctttac cgccccaaac
    21781 atatggagta ccaatatatt tgtaagcttc tgctacgatt gctgcaccat tcacacttcc
    21841 agaaggagct ggagcaggtg ttacaggttt tggttgttca ggtgttggtg ttggcgttgg
    21901 agccggagta ctacttcctg tgccgtttcc tgtatcaaca ctttgatctg ttgatggtgt
    21961 tgtaggcgca ggtgttgttg attcttctgt tgttgaagat tcaggcactg ttgttgattc
    22021 ttctgttgct gaagattcag gcactgttgt tgattcttct gttgctgaag attcaggcgc
    22081 tgttgttgat tcttctgttg ctgaagattc aggcgctgtt gattcttctg ttgaagattc
    22141 aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct gatgatgatt cttcagtcat
    22201 tgaagattgt gtagctgatg attcttctgt tgctgagctt tgtgcagtag atgaagcttg
    22261 agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct ttttcagctt tttcttgtgc
    22321 agcttgttga cgagcttgtt ctgctagacg ttgttgttca cgaatacgag cttgctctgc
    22381 ttcagcttct gctttttgac ggttcaagtc agctttcttg tcttcagcag ttgcttgttc
    22441 agcagctaat gaagttttca gtacgttcaa atcagcttgt ttagataaaa gatcgccttt
    22501 ttgagattcc aaagcagctt ggttttcagc taattctttt agtttagcat cgttttcagc
    22561 ttttttgtct tctacagctt ttttatcttg tttttgttgt tccatcaagt cgttgtttgc
    22621 ttttaccatc gttgtcattg cttgaacgcg tccaatagca tctgctaatg aatcagcatt
    22681 caatacagca tcgatgtagt ttgagcttgt gttgcttact tgtgcttcgc gtgcttgttt
    22741 ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa tctgcaatgt cttttactaa
    22801 ttgtgcagat tcttgacgca atgtatcttg ttttgctaat aggtcttgtg cttgtgtgtt
    22861 gatttcagaa acttggcttt ctaaggcatc gatttgtgat tgtgcatctg cttgttgatt
    22921 tttcaagtct gcaatctttt gatcttgctg ttgtatctga gaatcaaaat cgtccgccgc
    22981 ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa cataccatta ctgctgatat
    23041 taaactcttt ttcactcttc attcctccga ctggcttaat ttaataaata tctaaatata
    23101 tttaagtctt tcttaacgat ataaaaattg taccatagca gtgtgtcaac gatataatag
    23161 ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga caaaactgtt cgtttcagaa
    23221 aatgatcatg aaaaagccgc gttaagtcta gcatttctta agataacaac gattcttttt
    23281 cgctttcgac ctaaaaataa aaaaagaaat tattcatcac tgaataattt cttaaaggga
    23341 aaaatgaata atacaaatat aatcatgttc aacagcagtg taggtcctaa aaaccttgta
    23401 ataaaatacg tgttatttac tactgctaat ttaaaaatca actggaccac catcgtaaac
    23461 aattcataac ccgtgacaaa gatgatcata ctgaaaaatt cagtaaagat gttgacatgg
    23521 ataacgtctt tcattacata catcaaccat acaattaaag ggagagctac tgcatagata
    23581 ccgataacgc caatatagta agcgtcatag atagccccga gaacaatcgt ggtaatcagt
    23641 aaataacgtt tttcaaacgc catactgcaa cataataatg ccaatatcag aaagtgggca
    23701 tttgacatat aggtgccttt cgaccactcc cctagcattc ttgttaaatg accatctatc
    23761 aacatcaaaa agaacaaaac gattggcaga tagtacttca ttgtttcttt cttcaacatg
    23821 gactaatcct ccgctagccg ttggacaatc gtcacaactg acacgtcata catttctgca
    23881 taaggtttca cataaacttc ccgatccagc ccataactat ctggtttcgt tttaataacc
    23941 gtaccgatcg gcaagttagc tggagaattc cctccaagac cggatgtctg gacaacatcc
    24001 ccttctttga tatccgtatc accagttaat tgggtcacca ctaaggcatg gagcttttca
    24061 tcatagtttt taagcaaacc aaacgcttcg ccattagccg aagatacccg tactggaaaa
    24121 tgattggagc tttcattaga ggatgacagt aattcgattt tagacgaagc cgtattgacc
    24181 tcgatcactc ggccaatcaa tcctttttgc gacatgactg ccatatttgc ttcgataccg
    24241 tctttagaac ccttgtctac gatcagcatg tcctgccaac tgtcaggcga acgagtaatg
    24301 acatttgccg ttactttttc gtaactagtc agcgtctgat tcaactcaag ttcttttttc
    24361 aacgcttcga tttctctctc gtaattcttg ttttgctgtg aaacgtcatc atagctatcg
    24421 attttttctt ttagccgctc attttcggca taagttgaaa aaagattatg gacagaatct
    24481 actccatttc gtacccaccg tactggggca gagattgttt tatcaaccat tgcaacactg
    24541 tcattaacga tggattggaa gaaattgggc tttccttcat tcgctcgctg cgcagcggtc
    24601 acacttagta tcgtcaccac gatgataaca ataaccaagg taataataat atttttatta
    24661 ggattgaact tcttcacaaa gacacctcga attaattaga ctcttgtgtt caattttatc
    24721 actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa gctttttttt agcaaataat
    24781 agattctgtc atttttagcc tgaaagacaa atttttttat ttgtttatgg atcaatcttt
    24841 gctttgaata actgcaatca taccatctga aaaagaaaat tctgcggtat ctgaaagaaa
    24901 ttcgttactt gcataagatg tcggatattc tacttcttta ttttccaata catacttgct
    24961 gccttttaat gtaagattcg agacgggagt caagcagcaa taagctaaat acttcatatc
    25021 tggtatctgt cttactatat gctctccagg caaataataa gacaaataat tttgacaatc
    25081 tctgataata agctgatggg caaagggttt gaagcgaggt tccataccta accagagatt
    25141 ggctaaaaga tgatccaatc ttccgcccgt agctccaatc aaagtgactt ccgcttgcgg
    25201 gtaacgttca aatgttttta agatacctaa ctgtgtatct gtatcgtctt tttctgcagg
    25261 ggaacgggta tactctttgg cttttgcaaa tacttccttc tgctcttcgt tcgacaatga
    25321 atcaaaatca cccactgcca gatctactgg gtagcctgct tgaagcaggt acagcgcacc
    25381 acggtctatc ccgacatagg catcgtattt ggacagcaga gactgcggcc agttttgcgg
    25441 attacctcca gcagctaata aaattttcat ttagtctaat gcctctttta atcgttcgat
    25501 ttgtccgact ggattttcgg ctccatagat atatgatcct gcaacgaaga catccgcccc
    25561 tgcatcttta cagatttttg ctgtttctgg aacgattccg ccatctactt cgattgcata
    25621 agtccaattg ttctctctac gtagatcagc gagttccgta attttttcta ctgtttcttc
    25681 aataaagctt tgtccgccga acccaggatt gacagtcatg actagtactt gatcagccaa
    25741 aggcaaaaca tgtttgatca tggagactgg tgttcctgga ttgataacta ctccagcttt
    25801 aactcctaag ctttttgtca tttgaagtgc ccgatgaata tgaggagttg cttcgacgtg
    25861 gacagtaatg atatctgctc cagcttttgc aaatgctgga atgtagtttt ctggatcaac
    25921 gatcatcaga tggacgtcta acggtaattt agtaacagga cggattgcct gaacaatatt
    25981 tggccctaat gtgatatttg ggacaaatcg gccatccatt acatctacgt ggatgtaatc
    26041 tgcgcctcct ttttccacta actcaatgtc tctttgtaaa ttggcaaaat ctgcactcaa
    26101 aatggatggt gctatcttca ttcttgtttc ctcctatttt cgtttacctt ctttttttgc
    26161 atacattggt tttctttttt cgatttcttg taagaattgc aaataattgt cgtaacgtgt
    26221 ctgtgcgata ccacctgttt ctactctatg ttttacttcg cagccaggtt ctttagcatg
    26281 catgcactct ctaaatttgc aatggacaga agcttctaca aattcaggaa attgtctcgg
    26341 cagttctcct gcttcaattg tcaaaaagtc aatcgagcta aatcctggtg tatctgctac
    26401 taacccgcca tagataggaa tcagttccac atgacgtgtt gtatgccgtc ctcttccaag
    26461 tgcatcagaa atctctcctg ttgctaaatt cagttccggt gatatttgat tcagtagcgt
    26521 cgattttcct gctccagact gacccataaa gaccgatagc cgttctggga agtaacggat
    26581 cagttctgtc gtatcacccg tatgattggg tacaatcacc ggatagccta ttttagtata
    26641 ggtttgcttg atctcttcga ttttttctgg gtcgtctgac aagtcggtct ttgtcaaaaa
    26701 aataatcggt tcgatcgact cgtattccaa ggtaactaga aagcggtcaa gtagattata
    26761 agaaaatgca ggctctacga tacttgtgac aatgattcca agatcaacat ttgtgactgg
    26821 tgggcgaact aattgatttt ttctcggcaa gatttccaga agatagccgt ctgtttgatt
    26881 ttcactctca aagatcacct gatctccgac aagcggcgtg attttccgat tccggaaatt
    26941 tcctcttcct cttgtttgat aggtctctcc gtttgcgtat acatagtaaa aaccgctgat
    27001 tgcttttctg atttgtcctt tgataagtgc cacctccgtt tttgtttatt ttaccatacg
    27061 aagaggaaag taggtgttat tccatgcaaa cttctcaaag tatatggctt gaaaattgat
    27121 caaaagagat agaaacacaa aaaagagaat gcaacaaatc aatgtcgcat tcccttctga
    27181 tatattttat tcagcactta ttttgaactt tctgaggtgc tagctgatga acttgtggag
    27241 ctggaagatt cttcttttga agagttgctt gtgctactgg agctacttga gctgctagaa
    27301 ctgtctgaag agtctttttc agaactagaa gacgctggat ctggtccttt agagtaaatt
    27361 acgttcacaa ccattccttt ggtaattgtc gttcctgccc ctggtactgt acgaatcact
    27421 ttatctttat ccaccgtgtt tgaatattcg gaagtctcat gcccgatata ttctgcaccg
    27481 acactagcta aataactttg tgcttcgctt tttgtatagc cggagatatc attcaattta
    27541 ggctctgttc cttggcttac gacaaaattg atgtaagttt cttctgctac aacttcaccc
    27601 gaggctggat cctgactgat gatcgaacct gatggtacag tgtcgctata gtcataagta
    27661 gcactaatat tattgatatt gaatccttgt gtaacaagcc aattacgaac agtttcgtag
    27721 ttttctccta catgactact caaagtcact gtttctggtc ctttgctgac tgtgaaagta
    27781 atcgtgtctg ttttaggatc gacttctgta tcttctgatg gagattgcga aatgatcatt
    27841 ccgctttcta cctctgaact gaattcctct tctttttgaa tcaagtcagc agaaaaccct
    27901 aattctttca aacttgcttc ggcatcttca taacttttac cactgtaatc atctaatttg
    27961 atttttttcg tgccgctact aatatacaga gtgacttcgc gtttttgctt gactgtcgtt
    28021 cccgctgccg ggtctgtctt cactacatta ccttcttcga ttttgtcatc tgcaatttct
    28081 tctgtttttg ttgctacttc taatttcgct tttgcgagtg tttctctagc agaagcttca
    28141 gaaagatcag aaacatcagg aacaacaact tctcctcgcc cgccagaagc aaagaataca
    28201 gctgttccga ttcctaaaac agcaagaata gctaatagga tcagccacca ttttttctgt
    28261 cctttctttt taggatcagg cagaacttct tcctcctctg gtaaggtttc gttttctggc
    28321 ttgtcttttg gcagaggcat agatttgaat gaatcaggca ttggtgcatc ttccgtaagg
    28381 ggtgtgataa cttttgtttc attatccatc acatgaggct gccatttttc ttcgtttgca
    28441 cgagctggtg ataatactgt agaaagatca tcagacattt cctcggctga cttataacgg
    28501 tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt ccagtgcttg tggcacattt
    28561 ggatcaagtg cttttataga tggaagatca tcttgaaaat gttttaaagc aattgttaca
    28621 gcagattcac catcaaacgg tacgctaccc gtcaacatct cataaaggat gatccctaaa
    28681 gcataaatat ctgactgctt cgttgccata cttcctctgg cctgctcagg ggacagataa
    28741 tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg tttcagataa agcgatagca
    28801 atcccgaaat ccgtgatttt tacaacacct tcattatcga tcagtacgtt ttggggtttc
    28861 aaatctcggt ggatgatttg atggttatga gctaaagaaa tagcagaaag gatctgctgc
    28921 ataatattta ctgctgtttc ataaggaaca ggataatgtg tttggatata tcgtttcaag
    28981 tccattcctt tgacgtattc catcaccaga tactgcatgt tgtcttcttc accaacatcg
    29041 taaacactaa caatgtttgg atgaacgagt tctgttgctg ctaaagcttc tctctgaaat
    29101 cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta agactttaac tgctacatca
    29161 cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca taccaccgct accgatattt
    29221 ccagtgattt gataacggcc atttaatttt ttgcccaact cgatcataca ctgtcctcct
    29281 tatattcgat aagaagaacg gtgatattat ccgctccgcc tgcttcattg gcacgatcaa
    29341 tcagctcagt tactttatga gacaatgggc cttcttgatt gatgatcgtt cctatttctt
    29401 cttcagacag catgttcgtc aagccatctg aacataacag cagtcgatct tttagttgcc
    29461 aaagatgagt agaaacatct acttcgactg ttccaggcat gccgacagaa cgtgttaaaa
    29521 tatttttcct tggatggttc actgccattt cttcgctgat ttctcctgat ttgactagtt
    29581 cattgactaa agaatgatcc tccgtcaatt gcatgatttt atcttcacga atcaaataag
    29641 ctctgctatc cccaacattg gcaatcgtga accagtcatc tgacagagcg gccgctacga
    29701 ttgttgttcc cataccgtta tattccggct gttcttgtcc acgctgataa atcagcatat
    29761 tttcttcttg aatcgattga atgaaccatt gggcgatttt ttcatcgtca acagcttctt
    29821 gtttttccca agcttcgcct agattcgtta ccgccatctg actggcaatg tctccggcac
    29881 gatgaccacc catcccgtct gctaatacag caagcttgag acctgaacgg ctggtaaata
    29941 cactggcata gtcttgattt gtgttacgtc gtcttcctac atctgattga tattcaatct
    30001 gcattttttc acctcattac tttttgcgca aacaacaaat gaaaaatcca tctgtgtaaa
    30061 actgatgcgg atacaaagtc aacattttct cttcgattga tgattgtagc ataggattgg
    30121 tcaaaacatc aatcctctca aaatcagggt gtctttccaa gaaagcagaa acaacgtctt
    30181 ggttttcttc ttttaagatc gtacaagtgc tataagtcag aataccggaa gactttaaag
    30241 ttggtgcgca gctttctaaa atttccaatt ggatttttgg taaattcgct aattcatccg
    30301 gctgtttttt ataacgaata tctggttttc gccgaagcag cccaagacct gaacaaggcg
    30361 cgtctactaa aatccgatca aactgctctg cttcgaactg tttactgact tctctagcat
    30421 ccatcttttg agcgtagaca gcagaagaga cacctagacg tgctgcatta tcttcaatga
    30481 gtttgacttt gtgttcgtgt acatctaaag cgaccacttc tccaccgcat gacggatcta
    30541 aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc acaggcatct aagacatgat
    30601 gttcaggctc gatttgcata gacggcgcta caagcatcga actttcatct tggatcgtca
    30661 tgacgccatc gtggaacaat gagctagaag ctaaatgccc ttttttcgct actataccgt
    30721 atggactgac ttggcttttt tcagctgtga tctcttcttc ttgcaaaaca gtgatggcct
    30781 gatctctgga aagtcggcgt gtatctactc gggcactagc atggctaggc tgatatagcg
    30841 ataaaccgag cttttcagtt tcttcgtatc cgatttgatc caccagtcgt ttcgtcatcc
    30901 atagaggcag gctgattttg acagaaagtc tttcgatagg atcagcaatg ccttgcagat
    30961 ctggtgctcc ttgacgctga taattcctca agacaccatt tacaaatttc cctgtcccag
    31021 gatttccttt tacttttgcg atttcgaccg cctcgtttaa aatagcatgt gcagggactt
    31081 tatctaaaaa ttctaattgg tatacagaaa gataaagaag catcttgacc cagtcatcca
    31141 ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc caatagtcgc tgatggctaa
    31201 tcgtaccata gatcaattct gtaaataaac ccacatcttt ttgatttagc tcgctttgtt
    31261 tgatcatctc attcaacaaa agattcgagt aggcgccacc cttgttcact cgctctaatg
    31321 tcagtaatgc catatatcgt actgattttt tcattttttt agggattttt ttcattattt
    31381 cacctgctga ccaatctgaa cttgctgacc gcttccgtta aggaaatcat gtacggcctg
    31441 ttttccttta ccagctggct gtaattcatt gatggctaag acggtctgtt taccgcaagc
    31501 aatccataga ttttttttgt ctttccggat gatcgtacca ggtgctttgt ctgtagtttc
    31561 tgccaatact tcaaccgata ataatttcca gcgtatgccc tcataggtcg tgaatgcaat
    31621 tggccaagga cgcattccgc gtacttggtt gtcaatttct tctgcagttt tatgccaatc
    31681 gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt gcttctgact cgtcttgagg
    31741 tactggtttt tgtccatcca aaatattggg caatgtttct agcaatagct ttcttcctaa
    31801 aaggctaagc ttttcaaaca tcgtccctac atcatcttgt ttagtgattg gtatgctttc
    31861 ttgcgcatag attccgcctg catccatttt tttgatcatt tccatgatcg ttacaccagt
    31921 ttctttctcc ccattcatga tggcataatg aacaggcgca cctccccgat atttaggaag
    31981 taaagaagca tggacattga ttgcccctaa ttttggtact tgcaataact tttcaggcaa
    32041 gaattggcca aatgccgcag tgacgatcac atcaggcacc aattcaatga tttcttccat
    32101 ttctggcgaa ccggagattt tctctggctg cagcacacgg ataccgtgct tcagagccgc
    32161 tttttttacc ggtgtcggcg taattactcg tttccgccca actggacggt cgggctgtgt
    32221 taccactgcc tggatctcgt aacctgattc tatcagccct tcaagaatcg gcacagaaaa
    32281 agcaggtgtg cccataaata ctagcttagt catcaagatg ttcctccata tattcttcaa
    32341 gttcttcttc cgggatctgc tgtatcattt tctcaatgaa caagataccg tctaagtgat
    32401 cgatttcatg ctggaaggca cgggctaaat agccaaaagc cgtcacttct atctcttctc
    32461 cgtctcgatc ataataacgc acggtcactt catctgctcg cttgaccgtt ccatagacat
    32521 gcggaatact gagacaacct tctacgtcaa ggctttcacc ttttgcttcg atgatttctg
    32581 ggttaatcag ttcaaatttc tcgccttcat ctacttcgat cacagcaatt ctcttgtttt
    32641 gtcctacttg aggagcagcg atccctattc cgtcattcgc aatcattgtc tcatataaat
    32701 tgtctaataa agcgattgtc tcgtccgtga ttacatcgat tggctgagca gttcttttta
    32761 atttatcatt agggtggatc aatataggat agcgcataat ttggcccttt cttaaataaa
    32821 gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt cgctgttctt tctggcttcc
    32881 attcaaaatt tcatccaaaa catgaggaag attcggttca tgtttatatt tgatgattag
    32941 ttgataataa tatcggtttt tcactctcaa aatcatgctg ggcgttggcc ctaatagcaa
    33001 gctttgaggg cttaacactt cttttagctg attggcaatc tgaaagattt tctttgccgc
    33061 tacttgttct tcaggatggc tggcggttat tttcacagta aagtaataag gaggatagcc
    33121 tccacgatgc cgtacatgca tttcctgctg ataaaactgc tcataatttt gttttttagc
    33181 caattctatg gcgtaatgtt gaggattgaa tgtttggatc acgacttcgc ctgctttttc
    33241 cgctcgccct gctcgtcctg aaacttgggt aagcaattgg aatgtatgct cactagaacg
    33301 aaaatccggt aaattcaggg aggtatcagc attcaacacc ccaactaatg tcacttctgg
    33361 aaagtccaaa cctttagcaa tcatttgtgt ccctaataat atatctgctt cttttgcacc
    33421 gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga gtggtatcta catccatacg
    33481 caaaatccta gcttgcggat aaagttctcg gagttcctct tctacttttt gagtacctgt
    33541 tccatagtag cggattttat tgccgccaca attaggacag cgattgggaa taggttcttc
    33601 atgtccgcaa taatggcagc gcattgaacg tgtatccata tgcagtgtaa gagagatatc
    33661 gcaattagga catggaagaa caaatccaca gtccctacac atcacaaaag acgaatatcc
    33721 tcgacgattg agcagtagga cagtttgttc ttttttgtct aatcgatttt gtattttttc
    33781 ctgcaaatta gcagaaaaag tagaggtccg atgattctga aactcttcac gcatatctac
    33841 gatctcaata gctggaagct gtgcagctgc tttggctcgg tggtttaact gcaataactg
    33901 ataaacgcct ttttgagctc gtgctcttga ttctaaagag ggcgtcgcgc ttcctagaac
    33961 tattgggcaa tgatgatatt tactgcgcca aattgctaga tctctcgcat gataacgcgg
    34021 tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg ataatgatca gcccgatatt
    34081 tttcagcggg gcaaaaatag cagatcgtgc gcctactact acttgcgctt cttcccgctc
    34141 aatcttgcgc cattcatcat atttttcacc ttgggaaagt ccgctatgta agaccgcaac
    34201 tgattctccg aagcgtcctt tgaatcgctc gaccatttgc ggagtcaatg cgatttcagg
    34261 cactaacata atagccgttt tattttctga caaaacatca gcgattgcct gaagatatac
    34321 ttctgttttc ccgctgccag tgattccctc cagcaaaaag acctgatctt tttgctgctg
    34381 acctgctgtg atgatttgtt cgacagcatt tttttgttct gcattcagtt ccaatgccgt
    34441 cgtttggtca aatactcggt ctttataagg gtcgcgataa cgttctgatt ctataaactc
    34501 cagccagccc ttttcttttc cttgattcaa gacagcagta ctgattcctt tatttttgaa
    34561 ataagcaatt ggttcttctt ggctcagttc agataaacac aaaagaagtt ctttttgttt
    34621 cttggcattt tgccgtaatt ttgcccattc ttcttcgatt tgcttttcag ttaacaaaga
    34681 acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg acttcatatt tgatatctac
    34741 taattgctct tggcgccagc gaatcaattg agaaaggctg ccttcttctt ctgcttcctt
    34801 ccaggaaatc acttctcgct gatcaaacaa tacctgaact tctggtgtgt ctgccaaagg
    34861 atagatcaat ttatcatatt ctgctttcat cacactcgga agcatcgttt gtagacaagt
    34921 gattttgaat gcgtaagtaa tttttttcat ataatcagct aatgaaagta attcttcatt
    34981 gaccaccgga gcaaggtcta gcaaacgaat aactgccttc aaagatggat tagctgattc
    35041 ttctgatttt tggatagcca taacaaatcc ttggacatga cgattgccat taccaaaagg
    35101 gacttctact cgcatcccga cttgcaaagc tgtttccatt tcagagggaa ccaaataagt
    35161 gaatggctga tcggtctgca tagtaggtac gtccacaatg acttcagctg ttaatgtcat
    35221 ggttcgtttc ctcctttttt tgtcttttct tatcaagcga caaaaagttc cttgcctatt
    35281 ttactagaga tcgatggatt tgtcttatct tctgccaaat cctaacaaaa aattaacagc
    35341 agataagaaa aaaaagaact gtgtcaaaaa gttcagacac agccttttct accagacaat
    35401 aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt ttctttttag atgttcgctc
    35461 tatttttctt tattacattt tttgctcttc acggatacgt gattccaatt cgtgttgttc
    35521 acgctctttt tgtgcttgac gttcctcttc ttccatcttc aaacgttcac gttttaattc
    35581 aggatgtgga tcattgacta catttcctgc atcgatttct tctaaagctt gaccgacgct
    35641 ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt gcatccaatt catgcgcgcg
    35701 tttacttgct aaaatcacta gagaatattt tgaatttaca cgatctaata gtgagtcaat
    35761 tgaaggtttt aacatcataa gactacatct cctttaacat tttaatatat ttaccgatta
    35821 cgcgatctac tcgaaaatgt tcactggcaa taatgttttt gattcgttca actgctagcg
    35881 gcacttcatc atttacaaca gcatagtcgt aaagagccat catttcgatt tcttcttttg
    35941 caactttcat tcgtttttcg attacttcat ctgcatctgt tcctcggcct acgatccgtg
    36001 attttaattc cgctaaatct ggcggagtca aaaagatgaa tacaccatcg ggcacttttt
    36061 ctttgacttg ttttgctcct tggacttcaa tctccaagaa aacatctttt ccttcatcca
    36121 atgtcttgtt gacatatgac aaaggagttc cataataatt cccgacatat tctgcgtatt
    36181 ccagcatctc tccagcttcg atcattgatt caaactcttc tttgcttcgg aagaaataat
    36241 caacgccttc gacctcccct tcacgcattt tacgggtagt cattgagatc gaatactgaa
    36301 aatcattatc ctcactctca aaaattgctt ttcgtactgt gccttttcct acaccggatg
    36361 gaccagacaa cacgattaat aatccacgct ctgacatgac gacgtccttt caactagtct
    36421 ttttgtactt atcgtttatt ttgcactttt tttctgcaac tttcaaggtt atctttaatt
    36481 atagctagaa attggtaaaa aagctaccct gtaccaagag gtcgtaaaaa agctgtcctg
    36541 catcaagtaa taagaacagc caaacataaa cagcttctca tgtttttcgt ttggttgggg
    36601 ttattatcgc agatgcaagc ttttgaacgc cgtgtataag aggtcgtgaa gaagccgatt
    36661 agtttcgagt atgaagaaaa attttgtgaa aatagtttct cacatttttg cataaatttg
    36721 gcttaatacc gagaaaatgg tttttgaaca tcacttattc aaaataaaaa aagattgtga
    36781 cacgaatttt gtcacaacct ttaaatgtat tcctagagca aagaagctaa gcgaagaaat
    36841 ccaattctgt ttatttattg tatttctttg ttcagcatcc ttgctaaaag cccatattgg
    36901 acaaagtatt cactacaccc atgatctcat cttttcgaga acgtgctttt tctgtcggat
    36961 cttctacctc ttctgtgtca attcgaacaa taaatttccg attcatcgtc acgtaagcac
    37021 cgtcgttgat caaaataaac agttcgttct cattatagtc tgcacaggca tctttccata
    37081 cgtcaaagcc gtttttttca gaagggatat cccgtttaac cacagttttt ccacttaatt
    37141 cgtggaacgt tacgtcataa tatttcatta tctctacctc tttcgtcatt caatattttg
    37201 tacttgttct ctgatttttt ctaagatcgt cttagactgg acgacttgat ttttaatttc
    37261 gattgcattt gatttggaac cgatcgtgtt gatttcccga ttcatttctt ggatcaggaa
    37321 atctaattcc cgacctacag gacattgtgc ttcgataagt tcatctagtt tttgtacatg
    37381 gatcgccaga cgatccagtt cctcgtgaat atctccgcgt tccaacaaga tggctagctc
    37441 cgtcaacaac cgttgctcat cgactttcgc tccaagaaat tcctccagct ttttttgata
    37501 ccttatttga aattcctttt catagatttc tacaaaacta tcgagttttt ttagtacttc
    37561 tttgaattct tcagtattct ttttcaacac taaagctaaa gcttgacctt ctttttctcg
    37621 actcgcctca atagaatcaa gtgcgctttt caatgtatct agagctaact gtgccaattc
    37681 ttccatgctc tcttccggtt tttcttctac cgttacgaag ttttcgtcca gcacgaagcg
    37741 ttccgccatc cgactaattg acacttgatt ttgactaccg tatcgttgtt gaattccgtc
    37801 tgtcaattga gtgataagtg aatcaatcaa ttcccagttg atgacgatct gtttttcgtt
    37861 tttgccaata taagctagat tcacaaatac ttcgattcga ccgcggctta actgctgttt
    37921 aataagctga cgaaaatcgt tttcaacaaa gttcaggatt ttaggacttc gtatttgaac
    37981 atctagaaaa cgctggttga cactttttat ttcaatatct aactgatacg cttttgtttc
    38041 gcggatggct ttgccaaaac cagtcatact tttcattcga cagggtcctt cctcattaca
    38101 tattttctat tgctttttgc agttgttcaa actcttcgtt cgtcaatgtc aggcctttcc
    38161 ccattttttc atggtctggc gcccaatctc gaagatcgaa ttttggcggg cgaccattcc
    38221 agctgactag gttcagttct ttacgccacc ccttgttgtt ttctgacaat atagcgattt
    38281 cttctacgat ttcatatgaa aattcttgtg ccatggttgt tcctccttga gataatataa
    38341 atataggatc agtaatgtgt caactggttg cttaagcaat tcatgctgca aacgatcctt
    38401 gcgttttcct tgtagcagat aatcaaaata aacagttttc gtgcgctcat tttgtatttt
    38461 ttgctggtat tttcgatacc ttcgccattc ttcagtactt aattgaccaa tttgtgccaa
    38521 taaatcatcc ggcatacttt ttatatcttg ttggcagatc atttgaatca tcatgtctac
    38581 ttgatagatt tcattgggca gtaaaaaatg tttttttgct aaaagttcta gcatcgccaa
    38641 tccgcatgtc tctggtacga ccctgttctt atataaatta cgcagttttt gccaaaattt
    38701 tatgcttttg aaaaaagata attctaactg aatttctttt gttaagtaag acaaaaatcc
    38761 tcgccagtat gttcttgcag gtttaacaga atgaaatgta taccagttcc catagtaata
    38821 gcctaatcgt tttttcattt ccagatagcc taattgtaaa tttaattggt tcctttggct
    38881 atcaaaaagc agaaagctgc ctagtgtcca caatgtttga catttccaat ggataaaaat
    38941 gtctggcata cgaatttttt tcgctgggcc tggcccttgc acatctacga caaatgcctc
    39001 tgttgctcct ttgttaatcg caatatcaat aggtattttg ttgcgatacc caccgtctgc
    39061 atatttattt tttccgatct ttcgataagc catagcggga taaaatgaag cagaggcgag
    39121 tatccaatct ggtatttctt ctgtttttgt ctgttggata tcagtcacta cttccgctag
    39181 ttctggcagt ttagtagaca ccgtatataa acgtattttg ggattatgtt tgagtaagga
    39241 caaatccaaa ttgtctttga ccatcttttc caatggagca gaagaaaccc cgccttcaat
    39301 tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag cgttcacgaa ggtcttcaac
    39361 tgcagccatt tctggtaacg caagcacttg tcttgtcgac agctttttcc ataaattcac
    39421 agcttttttc atatctcctt gtaaaatcaa tgcgccgttc aacgcaccta ccgaggtacc
    39481 tgtgataatc tcaaagttga tatcatgttc cttcagcgcc tgccacacac caatctgata
    39541 agcaccgtgc gctcctcctc cacctaaaac aagtgctgta tgatagacca gctttttatt
    39601 taattgtgtg gttccttgat agccgttttt atgaaaaata cgcaaccatt cttctgatag
    39661 aggagattga aataaaaaac aacagttttc tttaataaaa aaacgagcgc aagcttccat
    39721 ataccgaaaa aattttttcc attcaaaagt ttcaccttca atcaacagat tattaaaaat
    39781 aacatctcct ctacttttag caatagtaaa atagacggta tattttttcg atactgcttt
    39841 aaaagcacat tgcttttttg cagaacgcca aaaagcgtct ctactttctt gatgactttg
    39901 aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa tacagcgaca ttttctgtga
    39961 aaagtaaact ttttttatct ccatcgcgct ctcctttttc ttcaagtata ccagtacgtc
    40021 ataaaaaggt catgagtctc gtattcgact ttgtagattc cataagaaaa caattcataa
    40081 aacctccttt aaataaaaaa atacaaaaat cacaaaaatt aattgaaaat gagagtgttt
    40141 tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa agaaacaagc ttttttaatg
    40201 caaatgttgt aaaataaaag agattaagag gaaataagag gaggaacatg atgactgagt
    40261 cagtttatgt acatattgat accacaagta atgctgtttt aacaaaaggg ctgactgcta
    40321 ctgactttgc aaacagtatc gtgcattttc ctcaaaactt gctcttgctt gatccatctg
    40381 cttctgctgg agaatatgaa tcccatacag ggctgaaagt aatccgtggt acagaaaata
    40441 ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt cgatgaattg aagtggatcg
    40501 actttactga tttaacgatg ctgaaagaat tgacaccttt agaaatttca gaattattat
    40561 attttggtca tatgaaaaca catcttcatt cgccgttttt ttataaacta cagaataatt
    40621 ttgtatactt cgatcttaat gatcagctga atcggattta ttatcgctat ttagatgaat
    40681 tttataggat ttttgctagt aaattaagtt cgatcttgtc agataagatc aatgtaaaaa
    40741 aatctttttt caaaaaagaa acacctgttg aaaaaatcag tgtggaatta ttgaaagaaa
    40801 tgcgtaattt gatgcaggaa gggatcattt tttcttttga tcaagttggt ttgatcgatg
    40861 gtaaatatcg tatcccaatc tatgtgattg aagattatat ttggaaatta aaaggcaccc
    40921 ggtatcgaga agaggaaatc atcgctactt taatctactc tgtcaccgat cgacactggc
    40981 aattggtaga agatgccgat ctttcttatc tgccattgaa gtagatctat ttcttatcaa
    41041 aaaataaaaa ggaaacaaga aactaggaca tgattctgtc tcaagtttct tgcttctttt
    41101 ttatttacag ctcctgtcgt ttatctggca caaaaatcga aggagtcgtt tgttggatcc
    41161 ctaataaaac gatgatcgtt acaatagctg tactcaaccc gctggcacca ttcattacga
    41221 aagaaaagag ccaaggattc atcccccata gtgcatacgc tccccagaaa accactcctg
    41281 caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa tgcaccaaac acgatgcttt
    41341 ttctaatttg tttcgtatta tcgtctacat agcctttttg gacatttgaa gccatgagcc
    41401 ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat caacacttga acgacactta
    41461 aataataaac ttgtcccaac ggaaaatgta ggatgcccca aatgaaacct gccagcatcc
    41521 ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg aatctgccct aatgaaatag
    41581 aaaaactgct gccgatctgg atcggaatca acgacaacac catcgctaac gctgcaacaa
    41641 tcgttccttc tacccaaact ctattttttc ccataaaaaa acttcctcct ttgaataaaa
    41701 aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac agctgtttat tcacaattcc
    41761 tacgctcgca taaccgatac aggtacgaag ggtttagaaa tagattcaat ctcagcccaa
    41821 ttggactccc ctttgtgagt ttatttatta tttatttttt gtttcaaaca atggactaac
    41881 tggtttgttt tcatggatac gtttgattgc atctcccatt agtccgccaa cacttacttc
    41941 atcgattttg tcgattttac gatcttctgg aagatagatc gagtctgtaa caactagacg
    42001 ctcgatagct gaatcttcta ttctttgtaa agcagggccg gataatactg gatgtgtaca
    42061 tgaagcatac acactagttg caccagcttc tttcaaagca ttggcagcca atgtgatcgt
    42121 acctgctgta tcgatcatat catcgatcag tacacaagtt ttgcctttga cttgtccaat
    42181 gatattcatt acttctgcaa catttgcttt tggacggcgt ttgtctatga tcgcaatcgg
    42241 agatttcaaa aattccgcta atttacgtgc ccgtgtgaca ccaccatggt ctggtgaaac
    42301 aacgacaaca tcatcgcctt tgattccttt ttctaagaaa taattggcaa taagtggcgc
    42361 acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg atttggactg catgaaggtc
    42421 caaagtcagc attcttgttg caccagcttt ttgaatcatg tttgctacta atttcgctgt
    42481 gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca tagccataat aaggcatgac
    42541 tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca atcatgatca gcaactccat
    42601 caagttatca ttgactgggc tgctcgttga ttgaataaca tagacatgcg atccacgaat
    42661 actttcttca atgtttactt gaatttctcc atcactaaat tgagtaacag atgattttcc
    42721 taactcgacg ccgactgctg ctgcaatttt ctctgctaat gggcgattgg aatttaacgc
    42781 aaaaattttt aatcttgggt caaagtaatg ttttgacatg gggacctcca ctttcttttt
    42841 cacaactcta gaattctacc taataaatag tagtcatatt ttttaaataa atcaagtgat
    42901 tttgcaaaag tcttagttta gataagggag tttttttgca tatccttctt tattgacttg
    42961 tcttgctctc gcaattgcca gcgcatattc tggaatattg tctgtaattg ttgacccggc
    43021 agcaatcgaa ctgtttttag ctacctcgac aggtccgatg atattcgtgc tagaaccaat
    43081 aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt ccatcatagt tgacgaagac
    43141 aacaccacat ccgacattga tttcttctcc cagcgtagca tcaccaacat aagtcaagtg
    43201 accgacttta gttcctttgc cgatttgagc atttttcact tccacaaagt ttccgatatg
    43261 gacgttttcg ccaatttcag cttttggtcg taaatgtgca taaggtccca catctgcatg
    43321 tttcttgaca tggctgcttt caatgactga gttttcaatg acgacgtgat cctcaatcct
    43381 actgtctacg atttttgaat gtgacccgat gacacaatca gaaccaatga ctgtatttcc
    43441 ttggatttgt acacctgctt cgatcaatgt gtctggaccg atttctacgc ctgcatcgat
    43501 gtatgtcgtt gctgaatcga caaagcttac gccattgacc atatgcatct ggttaattcg
    43561 ctttctcatg atctcatttg ctttagctaa cgcgatccga tcattgactc ccattgattc
    43621 ttcaaaatca tccgtttgat aagcagcaac tgtatggcct tcttctttta agatttcgat
    43681 gatatctgtt agatagtatt caccttgagc attgtttgtt ccaactttgc ttaacgcatc
    43741 gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg atttcttgta caagagcttc
    43801 ttctggtgtt gcatcttttt gttccacgat tttttctacg atcccaatat ggtcacggat
    43861 gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc aagatcgttg cacttgcatt
    43921 ttttccttgg tgatattcaa aaagattgtt cagcgtttca gtagtcaata atggtgtatc
    43981 gccactgata actaacgttg ttcctttttt tccttccaag aaagaagcag cttgcaaaac
    44041 agcatgtcct gttcccaatt gttctgcttg taacgcatat ttactgcgtt ctcctagttg
    44101 tgctttaact tgttctgctc cgtgtccaac gatcgtaatc acttcatctg gtttcgtttc
    44161 actgacccga ttaatgatat gttctaccat aggtttacca gaaacgggat gtaaaacttt
    44221 atataatttt gacttcatgc gggtaccttt ccctgccgcc aaaataattg cataacgtgc
    44281 gtccaactgc gtcactccta aatatagttt gctgatttca gtttatccca gtatacccat
    44341 tttttcaaca aaacaagtga ttttctttta tcccagcgtt ttctggataa aaaaatagat
    44401 agaccataac gggcctatct atttggtaac cggtgctctc ctgccaactc ctcggtcaca
    44461 agtcacacta ctcataaaac atgaaaagct gttttttgaa atcgtttctt gtgacttaga
    44521 gttaaacggt aggcttacaa cctcttcatt aaacgttttt caatcagctg accttcggta
    44581 ttagctcaat aaacggcaaa atatgaggag caatttttcc gtttattgag ctaatactca
    44641 ggacaggacg ctgatttcac aacctctaga taaacggtgt tcaaaagcca gtttctcggt
    44701 attaagccaa atttatgaaa aaatgtggaa agccattttc acaaaatttt tcttactact
    44761 cgaaactaag cggctttttc acaacctctt attctgaaaa ataatttcct ggcacaaccg
    44821 taatattttt cgtttgagta tctacatctt tgacataaag cagtgacgta taatcatcga
    44881 tggcgcgatg accgttgaat tttgattcgg caaatacagt gatgccaaca agttctgatt
    44941 cgaattcttc aatcaagctc ttcattccgt tgactgttcc gccgcctttc atgaaatcat
    45001 ccactacaag gacacgtgaa ccacgtttca aacttctctt agacagttcc atcttttcaa
    45061 ttctttcaga agatccagac acatagttga cacttactgt tgatccttct gttatttttg
    45121 agtcccggcg tacaataacg aatggcgcat tcagataata ggacacagct tgggcaatag
    45181 gtacgccttt tgtggctaca gtcattacag catcgatttt ttcaccaagg tatttagaag
    45241 caatgattct tccaacttga cgcagcagtt caggttctcc taacaaatca gagagataaa
    45301 catatcctcc tggaagcaga cggtcttgtt ctgatagacg ttctgctaga gattcaatat
    45361 attttttcgc ctcttcatat gaaatttcag ggatgaacaa tacaccacct gcagcacccg
    45421 ggattgtttc caaggttcca taccctcgtt ctttaaatgt tttcttgatg attcccaaat
    45481 cttcactgat agaagattta gcagattcat aacgttcggc aaagcttgtt aaggaaatca
    45541 attcatgcgg gtgattcaat aaatactgtg tcatgtcaac tagacgttca ctgcgacgta
    45601 ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt ttacattata taagtttttc
    45661 ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag ttcggtattt attaaaataa
    45721 aaagaagcct ataaaaaatt tttcgttttt ataaggcttc ttttctttat aatttagtta
    45781 ttttttcttc tgaaggcaac caatattcgt ttgatgatat ttacaatcag gaacaaagca
    45841 ataaagacta atgtgattgt tgctcctgga ggtgtatcta gataaaacga gctagttagt
    45901 cctgacagca tccctagaaa tcctacacaa acactgatag caatcacgac attgaagctt
    45961 ttgcctaatc gcataccgat tgctgcggga agaaccatga ttgcggatat caaaagtgca
    46021 ccggcaatcg ggatcatcac agcaatagcc acaccagtaa tcacattgaa catcatcgac
    46081 atcaaatgaa cgggtagccc atccacgtgg gcggtatctt catcaaaagt cagaacatac
    46141 atcggccgtt tgaataaaaa gaacaatata ccgatggcaa cgaataagat tcctaaaaag
    46201 atgacttgct cccatgtaat ggtcacgatc gaaccaaaca gataggattg gatacttgtt
    46261 gctgaattcc ctccgctcaa attcatcaat actaacgcta gcgccaaacc accagccatt
    46321 aaaatggcaa tggagatttc tgaataggtt cggtagatcg ttcgcagata ctccaatatc
    46381 acagctgcta atacaacgac tagcatggtc gtaagtgtag gattaaggtt caaaaagaat
    46441 cctaacgcta cccctgctaa agagacatga gaaagcgtat cagccatcag cgattgtctt
    46501 ctgatcacta aaaacacacc tagcatcggg gcgattccgg caatgaagat agcggcaaga
    46561 aacgctcttc tcatgaattc atatgaaagc aacgccattg agaatcctcc tttctcacca
    46621 gtcggatctg tctgtccgca tactgtttga tatcttcatg atcatgggtg atcattagaa
    46681 ctgatttccc atggacatgc gcactatgtc ggagaagacg ataaaattca tttctggatt
    46741 cttcatccat acctgttgtt ggttcatcaa gaacaaataa gtcagggtca gtagcaaaaa
    46801 tacgcgctaa actgattcgc tgcttttgtc cgccagacaa ttcacctatt cgccgatttc
    46861 gcatatccca catccctaca gcttctaaag cacgctgtac atgttgatga tctttttcag
    46921 agagacgctt gaaccagcgg cctcttggat atcttcctga acggactaat tcgatcaccg
    46981 tactaggaaa acctgcattg aaagatgcaa cttgttgagg aatataaccg atacttaatt
    47041 tttttccttc ttgatttttc tcagcaatct ttatcattcc ttttgaaggt ttcagtaatc
    47101 ctaacgatgc tttcaccaag gttgacttgg ctgctccatt ttctccagtt aaaatgacaa
    47161 attcacccgc atctacatga taagagacat cttctagtac aggttcatca tcatagtaaa
    47221 aggtcaaatc agatatttcg atataacgca ccttacaacc tccattctaa aacttaattg
    47281 atactttctt ttaaagcttt cagatttttt tccatgacag aaatataatt ttcgcctgct
    47341 ttgatttgtt cattagtcaa gctttctaaa ggattcaaca cttctagctt cactcctgtt
    47401 tcattcgaca atgtctcagc taccttcgaa gaggcatttt cttcaaaata aatcactttg
    47461 atgttgtttt tcttcacaaa ttcttttagc tcagctaagc gacttggtgt gggttcctga
    47521 tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt attgattcgc taaataagca
    47581 aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat cttttagctc atcggagtat
    47641 ttttgatcca gtgcttcaag ctttttgata taagcatcgg cattcttcgt aaaagtttcc
    47701 tttttatctg gatatttttc gcctaattct tcagcaatcg ttctgacttc ttttatagct
    47761 aataccggat cgagccaaac atgtggatcg tattcatgtg aatgaccatg cccttcttct
    47821 gattcttcat gttcgtctgc ttcttgcgat tctaaacggt cgattccttt agctgcttcg
    47881 atcatcaagg tctgtttgct atccactgaa tcctttaacg aatccacaaa aaattccatg
    47941 tctgaactat tatacacgaa aacatctgca tcggaaattt ctgctctttc ttttgcactt
    48001 ggttcgaaat catgcggctc cgttccagca gggatcaaaa gtttcacgtt tccttcatcc
    48061 cctacgatct cttttgtaaa atcatacatc ggataaaaag tcgtaacgat cgtcaaatcc
    48121 tcttttttgt ctgcttcctt gttcgtattt ccacatgctg caaacagtaa agcacctatc
    48181 attaccgtag ctgtcaaaag atattttttc atttatatgc tcctttcaat gaacaaatcg
    48241 taattttacg atttattaac gtgaactaat gtatcaaaat aaaaaaacac agtcaataga
    48301 aatccatcaa tttctgattt aagcatgtat attcttgatt aaatcgtaaa atggatgagt
    48361 tgctgctcat atctagccat ttcactacaa taaaaaactg aagaatacca ttttaggatt
    48421 cctcagtttt tcatcaccta ttttacttct tatattttat tgcagggttc gtactaaata
    48481 aacttcctca caaaaacctt ttaacccatt atatactctc tgcgctcttg aataatgttg
    48541 acacaaggca aaaacggttg gtccgcttcc actcatcaat gcagcatctg ctccgtactt
    48601 gatcatacgc tctttgattt gatggatgac tggatgccgc tttcccgtta tatcttctaa
    48661 actgtttccc atttttttga tcatctgctg ataatcttgc tctcggatgg cttcagcaag
    48721 tccgtggatg tcttgatgtt ggatggcttg cgtatctact tctctaaaca ctgtacgtgt
    48781 agagacactc attcttggtt tgaccaacac aacccagcac tgtggcatag aaggtaatac
    48841 ttggatttct tcccctttgc caccgacaaa tgccgtactt ccgtagacac agtaaggaac
    48901 atctgtaccg acttccaaac cgatttctgc cagttcttct aacgaacagc ccagattcca
    48961 taattggttg atccctctta gagcagctgc acagtctgta cttcctccgc ctaatccagc
    49021 tgctactgga atattcttct taacaaagat cttgacaccg gttttgatat tgtatttatt
    49081 ttttagcaat aaagcagctt gataaacatt gttccgttca tctaccggta aaaatgcttt
    49141 atttgtttct acaatgattt tgtcttctgt tatttcttca aaaaatagat gatctgccaa
    49201 gtctacgctc gacatcacca tttccaactc atgataccca tcttgccgtc tatataatac
    49261 atccagtcct aaatttatct tggctggcgc tttttcaatc aattccatcc tctcacccat
    49321 tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa ataaaagtat agcttatgtc
    49381 tgcctagtgg ttgattttgt catcgtttca acagctttca cagaattttc tcttcataat
    49441 ccaaacaagt tctagtgatt cttataattc tgtgagcact tcttctgcca aactcggaac
    49501 atttcctttt tcttttccta tcagactgag tagctgcatg gtagcaaatc cgaaattact
    49561 tcctttccta taagcaaaat aacgtccttc ccaatgatcg acatatttta atttcttttc
    49621 ataagcagac tggaaggaat agaaaaaatt accataatta tatacgatat gcatgacttt
    49681 ttcttgaaag aaagaaaaat ctgtttctcc aacattagct aatggagcta agcctagatt
    49741 ggcttccgag tagcccattt tctcgtatac aataaacaga ttggctaaca gatagtcagc
    49801 taattcctca gattctttag gtaagacccg caacaaatca tatcccgcct gcttcttcgt
    49861 catcggctga gcggtgatga agccaacgac tttttgatct ttacggacaa taccgacacc
    49921 gctagataat acatatgatt tatcaaatcg tcccacagag aagtattttt ctcgtgcacc
    49981 ttgaagccat ttttgtgaaa cttcatttac agcttgatgt aattcttccg gcagttgatc
    50041 gtaccaagta aaatggtatc cttctttcat caaacgttga tattcaagtt gctgactcag
    50101 ccgagacaag tcttctgttg ctagatcaac aatccctgct tcacctactt tcgtgaagtg
    50161 aaaacccaat tcatgaagca tcactacgta gctttctgat atcttataaa acgcaagctg
    50221 atagcctaaa caatctgctt gtttcataaa ctgattcgtt gctgcccgca tcattttagg
    50281 attgccaact ggatctccta aaacaaagca tttgtttcct ttgatctgat aaccaaaaac
    50341 gacttgatct tggtcgtctt cttggtaata aaaataactg taacctgaca agcgaagctg
    50401 atggcttgta gctgtgcctc cgtatgtttc caataaacgg tcaaaacggt ctttttcgta
    50461 ctcactgcca agcttcgtag tatcttcagc taaatattga taaagcgcca ctaaagctag
    50521 caatgagatg cccaaaccaa taagccctga gaaccacaca tcatctgaag gaaacaaaat
    50581 aaaatgattt cctgttattc gaccgactaa aaagcgtccg ctgtgatagc ctgcaattgc
    50641 gtaaacgata aacaaaaagc cgaacaagct agcatcaaag attatagctc cccaagaata
    50701 aacgaatttt gttcgataaa attcttttct ggataaccac acagctaaca gaatcaagac
    50761 atataccaat atcaactgcc aagataacgt acgtgtgatt gtatttaaaa taccaaaaga
    50821 tagtaaagca atcgtaggca gaaatgcctt tttgactttt actgaaagtg ctctagcaag
    50881 gccgagtaat aaaaagccaa ccaatagatt cactgtctga tctaaaaaat tgaatgagaa
    50941 cggcaataat acttgaaata gccgactgat tgcagacaga ttagtgattg ttgatagaag
    51001 taccatcatg atccccgcaa aatagacagc acttacgagc acgatatgcg caaccttttg
    51061 gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt ttgactcctg tatgtgtgat
    51121 gaacaataga agtcccgtca taaaaggtag aacataataa aacaaccgat aatataatag
    51181 ccaaactact gctacatctt gccgaacaga caattgactc attcctaaga tcatcaaaac
    51241 gtcaaaagtc cccattccac ctgggaccat cgtcagcatt cctatcaatg tagcaatcat
    51301 gaacatagga tagatcgaaa gcatcgaaat atctattctc atcaacttgc cgatgatcaa
    51361 aaataccgca agggctcccg tccattgtcc aaaggatgcc agtagcagac caagcaagcc
    51421 ttttggataa aactcgaaga acaatgtctt tctctttaga taagcaaaaa agaaaatgac
    51481 tggtgctaaa aaactaccgc cgaaaagcca gatccaatag cttcgaaaaa tactttctga
    51541 ctgaataaaa aagacatcaa taaatgtaag aaacgaccat acagacaaac cagtcagcat
    51601 aaatagtgcg acttttgata cagtggctaa aaccattttt ctatttgtct gctttccata
    51661 aaaactagcc cgcagacttg ctcctaccac accaccaaaa ccggcgagat tgttgatcgt
    51721 gtttgttacc cacgcagcaa gaagaaactc ttttctttcc atttttggtt ttccttgttt
    51781 ttctaagact ctgatcgtca cccaatcata caacaacatc ggagtgacac ccagcagtcc
    51841 agccagaatc atgagcagga tcgtcatgcg gctttgttgt cccatccttt gccaaatatc
    51901 ttgccaactc atgccattag caatatttgc gacttgattt gccacaaaga ctaacacgga
    51961 gccgaaaaaa atcagtttta aaatcagact atgttccttt atccattgca gcatcaagta
    52021 tcgtttattt ttcacatttt cccattccta acttgtatac ttttgtgtca taccccaatg
    52081 ttatcttatt tattttaatt tttccacttt agacctgttc tttgcttgcg atgatagaaa
    52141 aagaaagata tcctaatgct aaaggagagg ataacatgaa agtaggattt atcggcacag
    52201 ggaatatggc acaagctatt attgatggtt tattaaaaaa aggaacagtc tcgaaagaag
    52261 aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa atttgctgct gaaactggcg
    52321 tccatgcatg caaaagtaat caggaagttg catcacaatc agatatcgtt cttttagccg
    52381 tgaaaccaaa aatcattgaa aaagtattgg aagaactaag agaatatagc cagaaagtct
    52441 tattcatttc tattgcttca ggaaaatcat taatagattt agaaacagcg cttgaagatc
    52501 ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt cagcgtagga gcaggcgttt
    52561 ctgcgatttg caaaaatgaa gctgccacgg aggaacagct tcagcaagct acgatgattt
    52621 ttgaagcaat cggcagcgtc tatcctttag cagaatcaga tttcagtaca ttcattggcc
    52681 ttgctggcag ctcacccgca ttcgtttaca tgctgatcga tgcgatggga cggactggtg
    52741 tactgaatgg tttgtccaaa cagacagcca ctgagattgc tgcaaaagca attatgggtt
    52801 cttgtcaaaa attcctttca agtgataaaa atccatggga acttgtagac caagtttctt
    52861 ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga agaagcagga ttcatcccag
    52921 caatcatcaa aggaatcaat acgacgatcg agaaagatct tgagatgttg aataaaaaat
    52981 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
    53041 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
    53101 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
    53161 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
    53221 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
    53281 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
    53341 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
    53401 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
    53461 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
    53521 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
    53581 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
    53641 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
    53701 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
    53761 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
    53821 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
    53881 tcatcgatga ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
    53941 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
    54001 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
    54061 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
    54121 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
    54181 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
    54241 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
    54301 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
    54361 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
    54421 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
    54481 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
    54541 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
    54601 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
    54661 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
    54721 aaaaacatgc acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca
    54781 aagaaacggc tttctactta ttcattaaac gatggttttt aaaataaatg acactagttt
    54841 catataggta aacctttaaa ttgagaaact tttctattaa attattcttt gtagatgaac
    54901 agatattttt attttatgta cccactcgta taattaaata actcacgatt tctttattca
    54961 ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag accagccgtt tttgtttatc
    55021 ataattttta ttcttctact aaaggcaatt cttcttgtcc tttttcatct tctgggacag
    55081 cgacttcttc accaatacca taagctgctc taactagagc agaaacttct gccatcattt
    55141 caggatgatt agccatatag attttagcgt tttcccgtcc ttgaccaatc cgatcttctt
    55201 tataagaata ccaagcaccg cttttatcaa caatgtcttt ttctactgcc atgtctagaa
    55261 gttctccttc ttgtgagatc cctaaaccat acatgacatc tacttcagcg attttgaaag
    55321 gaggtgctac tttgtttttt acgactttga tctttgtacg gttaccaaca atatctgtac
    55381 cttgtttcaa ttgttctgca cgacgtactt ccaaacggat cgttgcgtag aatttcaatg
    55441 cgcgtcctcc aggagtgatt tctggattcc caaacatcac gcctactttt tcacggattt
    55501 ggttgatgaa aatagcgatt gtttttgttt tgttgatcga accagagagt ttacgcaatg
    55561 cttgagacat caaacgtgct tgtaacccga catgtgagtc acccatttcg ccgtcgattt
    55621 ctgctcgtgg aactaaagca gcaactgaat cgactactac gatatctaca gccccacttg
    55681 atactaaagc atcagcgatc tctagacctt gttctcctgt gtcaggctgt gaaagaagta
    55741 gttcatcgat attcacacct aatttttgtg catattgcgg atctaacgca tgctcagcat
    55801 caatgaaagc ggccgttccg ccattttttt gtacttctgc aatagcgtgt agtgcaactg
    55861 ttgttttacc tgaactttca ggtccatata cttcgatgat acgtccacgg gggtaaccgc
    55921 cgacacctaa agcgacatct aacgcaagag agccacttgg aattgtagag atttgttggt
    55981 cgattttttc gcccaatttc ataatagagc ctttaccata gctcttttca atttttttca
    56041 gtgcagcatc taaggctgct ttacgatcat ctgccaatac gataatcctc cttatattta
    56101 caaatcagag caacatttca ttatccttat catatctttt ttagtgaaaa aaagcaagaa
    56161 aaaaccgaac aaatattcgc atttatcatt ttcttaaaag ctgttttctc aaaagatcaa
    56221 atccttgcat aaccgcatta tatctgatag cagcacgatc tctggcaaag tgatattttt
    56281 ccgaaaccac gccgtcttta gatgaaatag cgatccatac tgttcctgca gattgacctt
    56341 ctaacgtgtc cggtccagcc actccggtga aagctacact gtaatctgtt cttgctatct
    56401 cacgtgcttg aatggccatc tgctccacac attctttgct gactgttcca tattctgcta
    56461 atagctccgg atcaatctcc agaaaaccag cttttgtttg gagcgagtag gtaacgaatc
    56521 cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa tgcggcttgg aaggcgccgg
    56581 ctgtcaaact ttccgctgct gttacagtct gcttgttttc tttcagtagt tccactacga
    56641 cttttgctag cgaattatca tcgccgtatc cataaaaata ttcccctact cgttcttgga
    56701 ttttttcttc taatgctaaa agagcttgat tccctgcctg aacatcattt gttttcactg
    56761 tcagtcgtaa tgtcacttca tttggtttgg catatggtgc gatcgttgga ttgatctgtg
    56821 tctcaatcag atcttttagc tcagtgacca gtcgcgattc accaatccca taaaagcgca
    56881 aaactttaga aattagtttt tcttcagaag gaaaacgttc ctctagtaaa ggacgtactt
    56941 gttccacaaa cattggtttc aactcattag gcggaccggg aagaaggata tacgcatgtg
    57001 tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg atttggtaga ggaaccccgc
    57061 cttcgataat ctgggactgt tgcagattgt ttttggtcat ttttcgatgg gttgtttcaa
    57121 aatatgccaa taattttttg tatccttccg tattttgtat gagagactta ccgaggtgag
    57181 ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc taatccgcca cataagagta
    57241 tgagttcact tcttgtttca gctagagcaa tcaactcttc taaacgctgc tgattatctc
    57301 ctacaactgt ttgataataa acttcgatac ctagatcagc tagctgttct gacaagaaag
    57361 tggcattcgt attcacgact tgtcctagta aaagttctgt tccgacagct attatttctg
    57421 ctttcattac ttcccgctcc cttatatagt atacgctgta tttttatatt tttttattta
    57481 aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc tccttgttcc aaataaacag
    57541 tgttctcctg ccaaaacctc ggtcataagt cacaatatac ataaaacatg aaaagctgtt
    57601 ttttgaaatt atcccttgtg actcagagtt agacggcagg ctcacaacct ctccactaaa
    57661 cgatacttac attgatcctt tgaacacacc gctgtttttg atgaagtaat ccacacctga
    57721 atagatagta aagatcagac aagcataaag catgatttga tctactggaa taccgatcag
    57781 gctgaatggg atattattga taaacagcaa gataatagca accatttgtg tagccgtctt
    57841 gacttttccc ggccaagctg cagccatgac ttctccatgt tcgacaagta gtaaacgcaa
    57901 gcctgtgacg gctaattcac gacagacaat gatcgctact acccaaccag gggcctttcc
    57961 ttggtcaacc aacatgataa aagcagtcat aactagcatt ttatctgcca acgggtccgc
    58021 aaacttgcca aaattagtga caaggcctcg cgcacgggca atttttccat ctaaccagtc
    58081 tgtgatacta gctaaagcaa aaataactgc acctactagc tgtgtgacag ccagtgtggt
    58141 gtctcctacg ttaaattgtc cccaatctaa aggtaaacct acaaccaaaa tgaaaatcgg
    58201 gatcatacaa attctaagta cagttaattt atttggtaaa ttcacactat cgactccttt
    58261 cttccctatt ctacgtgact atcagcgaag tttccaccga tattatttac ttttccaaat
    58321 gaatttattc aaaggagtat ttttattgaa attgatcttt ttgaattaac gaagaaggac
    58381 cgcgacacag atttgtcacg atccttcttt tttataccta caagtaaaca gatgcgtaaa
    58441 ctaatttata cgatcattct gcgtattgaa gagtaaggtt cacatttttt ggactctgat
    58501 tgttttcccc tggattaact ggtacggatt ctccattcgc tttgatcgtt gcattgcttg
    58561 ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg taacacggta ctctgtgttt
    58621 catttgctgc cagtgtccct tgataaacat aggcattgtc tactagcaca cctaaccata
    58681 cacgattgac agcagtaaat tcaagggtta caggcttggt agcgtctgtg atatttactg
    58741 cgatggcact gccagaatct tctcctgtag ctattgccat tttcttttct tcacttgatt
    58801 ttgttgttga ttctactgta gaagactctt tggtctgcgt gctttgctct gaggaagagg
    58861 actcctcgac tgatccttct actttaagcg aagaagctgt gtctccgatt attggatcag
    58921 cttgacgatc ttgccaagtc atatagccca cgacaacgac aatcgccaaa gcaaccaatc
    58981 ctaataaaat gacaggaaga cttgtccaaa agcgacttgg atgcgattct tcctgatgca
    59041 ttgcttttct agaagtactt actgtttcag gctgcggacg ttcagggacc gatcctgtca
    59101 atttgctttt cccatcaaaa acatcgacta aatggtctcc atcttctcct acagcttcag
    59161 cgtattgccg aataaatgcc cgcacgtaaa atttaccagg caattggtca aaatcatcat
    59221 tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat ctgctgtaat tcatctaaag
    59281 atattttttt attcaggcgt gcttgccgta atttttctcc gatggtttca aaagccactc
    59341 gttcatcttc tccagtctgt ttgattctcc gaagcatctg ctgttgcaac gaaagacgtt
    59401 atgtttgggt gctgtgacaa aagtcatgtc acagctcctt ttttccgaat aaacattgtt
    59461 ctcctgccaa ctcctcggtc acaagtcaca atatccaata aatataagaa gctgtttttt
    59521 gaacttgttc cttgtgactc ggagttaaac ggcaggctca cgacctcttt cttaaacggt
    59581 gttcaatcag ctgttcctcg gtattagctc aaaaaccggc aaaatataag gagatatttt
    59641 tccgtatgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tccttaaacg
    59701 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca ttaagaagct
    59761 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttggta
    59821 cacggtgttc cagaagtagc ttcttcggca ataagccgaa atatcacaaa aatttgagaa
    59881 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcttt
    59941 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
    60001 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
    60061 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
    60121 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
    60181 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
    60241 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
    60301 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
    60361 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
    60421 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
    60481 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
    60541 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
    60601 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
    60661 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
    60721 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
    60781 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
    60841 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
    60901 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
    60961 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
    61021 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
    61081 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
    61141 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
    61201 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
    61261 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
    61321 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
    61381 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
    61441 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
    61501 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
    61561 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
    61621 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
    61681 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
    61741 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
    61801 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
    61861 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
    61921 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
    61981 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
    62041 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
    62101 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
    62161 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
    62221 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
    62281 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
    62341 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
    62401 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
    62461 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
    62521 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
    62581 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
    62641 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
    62701 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
    62761 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
    62821 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
    62881 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
    62941 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
    63001 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
    63061 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
    63121 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
    63181 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
    63241 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
    63301 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
    63361 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
    63421 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
    63481 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
    63541 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
    63601 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
    63661 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
    63721 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
    63781 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
    63841 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
    63901 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
    63961 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
    64021 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
    64081 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
    64141 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
    64201 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
    64261 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
    64321 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
    64381 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
    64441 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
    64501 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
    64561 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
    64621 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
    64681 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
    64741 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
    64801 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
    64861 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
    64921 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
    64981 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
    65041 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
    65101 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
    65161 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
    65221 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
    65281 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
    65341 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
    65401 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
    65461 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
    65521 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
    65581 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
    65641 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
    65701 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
    65761 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
    65821 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
    65881 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
    65941 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
    66001 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
    66061 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
    66121 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
    66181 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
    66241 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
    66301 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
    66361 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
    66421 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
    66481 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
    66541 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
    66601 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
    66661 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
    66721 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
    66781 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
    66841 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
    66901 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
    66961 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
    67021 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
    67081 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
    67141 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
    67201 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
    67261 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
    67321 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
    67381 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
    67441 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
    67501 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
    67561 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
    67621 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
    67681 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
    67741 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
    67801 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
    67861 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
    67921 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
    67981 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
    68041 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
    68101 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
    68161 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
    68221 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
    68281 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
    68341 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
    68401 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
    68461 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
    68521 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
    68581 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
    68641 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
    68701 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
    68761 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
    68821 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
    68881 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
    68941 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
    69001 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
    69061 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
    69121 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
    69181 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
    69241 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
    69301 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
    69361 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
    69421 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
    69481 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
    69541 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
    69601 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
    69661 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
    69721 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
    69781 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
    69841 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
    69901 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    69961 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    70021 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    70081 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    70141 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    70201 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    70261 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    70321 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    70381 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    70441 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    70501 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    70561 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    70621 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    70681 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    70741 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    70801 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    70861 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    70921 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    70981 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    71041 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    71101 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    71161 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    71221 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    71281 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    71341 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    71401 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    71461 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    71521 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    71581 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    71641 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    71701 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    71761 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    71821 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    71881 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    71941 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    72001 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    72061 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    72121 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    72181 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    72241 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    72301 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    72361 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    72421 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    72481 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    72541 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    72601 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    72661 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    72721 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    72781 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    72841 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    72901 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    72961 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    73021 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    73081 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    73141 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    73201 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    73261 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    73321 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    73381 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    73441 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    73501 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    73561 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    73621 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    73681 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    73741 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    73801 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    73861 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    73921 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    73981 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    74041 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    74101 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    74161 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    74221 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    74281 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    74341 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    74401 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    74461 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    74521 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    74581 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    74641 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    74701 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    74761 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    74821 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    74881 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    74941 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    75001 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    75061 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    75121 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    75181 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    75241 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    75301 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    75361 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    75421 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    75481 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    75541 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    75601 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    75661 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    75721 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    75781 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    75841 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    75901 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    75961 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    76021 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    76081 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    76141 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    76201 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    76261 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    76321 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    76381 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    76441 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    76501 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    76561 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    76621 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    76681 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    76741 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    76801 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    76861 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    76921 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    76981 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    77041 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    77101 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    77161 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    77221 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    77281 tgtttctcta atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa
    77341 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt
    77401 tgtttctcta atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca
    77461 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    77521 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct
    77581 tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact gataagaaca
    77641 atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca
    77701 ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg
    77761 tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct
    77821 tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct
    77881 cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc
    77941 gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc
    78001 cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca
    78061 tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga
    78121 agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc
    78181 tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc
    78241 ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca
    78301 gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact
    78361 aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag
    78421 ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag accagcgaat
    78481 acaacgatga taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag
    78541 aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc
    78601 gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga
    78661 cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc
    78721 tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct
    78781 ttctctctat tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt
    78841 ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac
    78901 tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa
    78961 gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa
    79021 gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat
    79081 ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc
    79141 cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa
    79201 aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa
    79261 aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt
    79321 tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag
    79381 agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat
    79441 tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga
    79501 acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact
    79561 atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt
    79621 gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag
    79681 cggcaatcac attcattttc caagcattca tataagcaga gaataattga tcaggtattc
    79741 cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc
    79801 atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa
    79861 tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag
    79921 gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg
    79981 cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt
    80041 tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt
    80101 taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca
    80161 gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt
    80221 tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct
    80281 aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct
    80341 cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc
    80401 agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg
    80461 gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga
    80521 gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa
    80581 ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc
    80641 agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg
    80701 tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt
    80761 gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt
    80821 tgtacactgt ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc
    80881 tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt
    80941 tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc
    81001 aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct
    81061 tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa
    81121 accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct
    81181 agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc
    81241 aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt
    81301 aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat
    81361 tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca
    81421 ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata
    81481 cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg
    81541 acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat
    81601 gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc
    81661 atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag
    81721 ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt
    81781 ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc
    81841 gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag
    81901 caatagctac tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg
    81961 ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg
    82021 gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac
    82081 caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta
    82141 agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag
    82201 gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag
    82261 cttggttgta gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca
    82321 ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt
    82381 ttgcttcctc gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg
    82441 cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat
    82501 catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca
    82561 ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct
    82621 aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct
    82681 gtatctttat ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct
    82741 acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc
    82801 aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg
    82861 ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt
    82921 ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat
    82981 aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca
    83041 acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa
    83101 taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg
    83161 aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata
    83221 gaagatagct cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt
    83281 tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag
    83341 ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg
    83401 cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac
    83461 tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct
    83521 tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga
    83581 tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa
    83641 taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt
    83701 gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg
    83761 ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt
    83821 ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac
    83881 atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca
    83941 ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta
    84001 gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc
    84061 tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga
    84121 tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc
    84181 atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta attaggggca
    84241 gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat
    84301 gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac
    84361 gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat
    84421 tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg
    84481 atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt
    84541 gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta
    84601 gctgactgca aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc
    84661 tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga
    84721 tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga
    84781 attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca
    84841 tatttaaata acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa
    84901 atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa
    84961 caagtaattc tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga
    85021 taatcatgtt gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag
    85081 ctatatcttc agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct
    85141 ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag
    85201 atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa
    85261 attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag
    85321 ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat
    85381 ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa
    85441 ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact
    85501 tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt
    85561 gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc
    85621 gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga
    85681 ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc attctattct
    85741 cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc
    85801 aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt
    85861 ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta
    85921 cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct
    85981 ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga
    86041 cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca
    86101 cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa
    86161 aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt
    86221 tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt
    86281 ccgcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga
    86341 cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac
    86401 ggacggctta tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact
    86461 tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt
    86521 ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc
    86581 aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca
    86641 ctcgaggctc ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa
    86701 aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct
    86761 ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc
    86821 ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag
    86881 atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga
    86941 caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga
    87001 tacatcggaa tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg
    87061 ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt
    87121 tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga
    87181 tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga
    87241 cggaaaaagt attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga
    87301 aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat
    87361 acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca
    87421 aaagatccaa tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat
    87481 aattccagta aattactata attataaaaa aagaattgac aaaacataac tgtctatata
    87541 aaattgatta cgttaacaat aattcgtttc atgtgactag atcatactag gcatgaaaaa
    87601 tctctgagga acatggtttc cctaggaggt ttttcgtgtc tatttttgtt tggccgcaat
    87661 caactggtct agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca tgaaaatcaa
    87721 aaagatcttt aatcaaaatg cagtattggt agatgacggt ggacaggaaa aagtagctat
    87781 tggaaaaggg attggatttg acagaaaaag aaatgatttg atttttgatc gtgatgtcga
    87841 acggatattt gtcatggaac cagaaggtca aatcaagttg caaaatttac ttaatcagat
    87901 tgatgaacgc tttttatttg cagcagaaac gatcattgat catgcggaaa ctgttttgat
    87961 ggaaaagttg aatgaacatg tattgatcgc tttgaccgac cacattgctt tttctgcgga
    88021 gaacattaat aacggtatcg tcatccgcaa caaactttta agagaaatcg aagtgcttta
    88081 cagtgatgaa ttcagtattg ctcaatgggc agtagagtat ctgacaaaag agcttgggat
    88141 tccatatact tatgacgaag caggatatat cgcaatccat attcacagcg gacgaagcgg
    88201 tcggaacaat aaccatcgca gtatccgtga agtgacgatt gtatctgaca tcatccattt
    88261 agtggaatca gaattggaaa cagatatcca ttcagaacca ttttcactga attattcaag
    88321 actggtgaat catttacgac tgcttctaca acgaacccat gcgcaacaat atgctgtttt
    88381 agacacggaa atcgtagaaa tggtcaaacg aaaataccca gaaagctata agatttctaa
    88441 aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg atcacgaaag aagaactggg
    88501 ttacttggcc atccatatcg agcgtttaag aagcgcaatc gtacaaacaa atgttaacaa
    88561 tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc ccttcaactt
    88621 ccagttgcgg tcttacctgc tgctgcttta ctagtaggga tcggaaactg gtgggcatct
    88681 tatagtaacg acattgtcgc tcatttttta caagctggcg gtaacgcagt tttaggtcaa
    88741 ttacctttgc tgtttgccgt tgggttagca ctagggatgt cagtggacaa atcaggtgct
    88801 gctgccttgg caggtttggt cgcatttgaa gttccagtaa atgttttaaa aactgattca
    88861 gtagctactt tattgaatat caaaccagaa tcagttgatc catcttttgc tcagatcagt
    88921 aacgtattta ttggtattat tgccggattg attgcggcag ctctttataa tcgtttccac
    88981 gaagcaaaat taccgatggc tttatctttc tttagcggca agcgtctagt gccaatcttg
    89041 gctgcctttg tcatgttgct gatcagtgca gtgctactgc tcgtatggcc tccggtttac
    89101 tctgctttag tttcttttgc taaattcatt ttgagtttag gctggggtgg tgcaggtctt
    89161 tatggcttct tcaaccgttt actgatccct acaggattgc atcaagcaat gaactccgtg
    89221 ttctggtttg acgtagcagg aatcaatgat attggtaaat ttttggcttc tgaaggaaca
    89281 aaaggcgtaa caggaatgta tcaagctgga tttttcccaa tcatgatgtt cgggcttcca
    89341 gcaggagcat atgctattta cagaaatgca cgtccagaaa acaaagcaaa aacagcttcc
    89401 ttgatgttag cagcagcttt tgcttcattc tttactggtg taacagaacc acttgaattc
    89461 tcatttatgt tcgttgcttg gccattatat gtcttgcatg caattttcac tgggatttct
    89521 ttagcagtga gtgcattctt ccattggaca gcaggatttg cctttagtgc cggatttgtc
    89581 gattacttcc tatctttgaa aaatccgatt gcgaatcagc cattgatgtt gatcgtccaa
    89641 ggattagtaa cagcagtgat ttactacttt ggctttaact ttgctatcaa aaaattccat
    89701 ctgatgactc ctggccgcga ggaagcagat ctttcagatg acgatactgc aacgactaat
    89761 tcagacaata aatatgcggc acaagcaagc aaaatctacg ctgcattagg cggagccgac
    89821 aatgtaactt cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa agatacaggc
    89881 ctgatcgatc aaaataaaat caaagccact ggcgtacctg gtatgaagat catcgacgga
    89941 aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg ttgcagatga gatggcaaaa
    90001 ctacatggtg gtgctgcaac acgacctgca caaacaaaca ccgtggttaa aacagaaaca
    90061 tttgccggtg gaacgatcga acaagatatt tacgctatcg caaatgggaa actgattcca
    90121 atcactgaag tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg ctatgctgta
    90181 ttaccagaaa atggagaaat cttctctcca atcgctggta cgatcacaaa cattttccca
    90241 acgaaacatg ctgtcggaat ccaaacagat gctggtatcg aagtcttgct tcatatgggc
    90301 atcaacactg ttgacttaaa aggcgaacca ttcactttat atgtaaaaga aggtcaaaaa
    90361 gtcgcaagag gtcaattgat tgctttagtt gatcttgccg ctattcaatc tgcaggcaaa
    90421 aacacggata tggtcgttgt attcacaaat ggcgacaaag tccaaagctt agaaatcgaa
    90481 cccgcacgtg atgtaaaagc aaatgataaa atcggctctg tttccaacaa agcctgagcc
    90541 atcgtcttcc ccagactttt ttatcgataa acaaacagga gctgagacaa aaatcttgtc
    90601 tcagctcctt tttccgaata aaaacggcgt tctcctgcca actccgcggt aataagtcca
    90661 gccaaacgta acatatgaag agctattttt tatttgactc ttcttattac ttgatgcaga
    90721 acagcttttt tacaacctct gataaacggt gttcaaaaac cagtttatcg gtatgaagtc
    90781 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttcata ctcaaaacta
    90841 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    90901 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    90961 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    91021 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    91081 ttttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    91141 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    91201 attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    91261 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    91321 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    91381 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    91441 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    91501 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    91561 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    91621 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    91681 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    91741 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    91801 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    91861 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    91921 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    91981 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    92041 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    92101 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    92161 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    92221 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    92281 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    92341 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    92401 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    92461 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    92521 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    92581 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    92641 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    92701 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgattacaa
    92761 caagatacgt tccttttggg aatgggatga ttcgggtagt gtttggaaca tcctgactcg
    92821 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    92881 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    92941 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    93001 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    93061 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    93121 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    93181 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    93241 cttcgactaa ctcataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    93301 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    93361 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    93421 gcatcaaact gcccttttct tccaagcggg ctccatcaaa tccataagaa acgttgatta
    93481 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    93541 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    93601 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    93661 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctgctga
    93721 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    93781 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctaggaga tcgcgattag
    93841 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    93901 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    93961 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    94021 ttatcttcct ccttgcttgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    94081 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    94141 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    94201 ataaattttg gggaagacac attctaagta aacaaagcgg taaaaatatc ttttattccc
    94261 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    94321 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    94381 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    94441 tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa
    94501 tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt
    94561 cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga actagatgaa
    94621 taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct atcatctgca
    94681 cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag tttctggcac
    94741 ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt gtgattgtct
    94801 atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg cttacgaaga
    94861 acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg tttcctccgc
    94921 tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc cgcaatttat
    94981 cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata aattaacaac
    95041 tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa cagcgaagtt
    95101 catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgctcattc agcaaaatgc
    95161 ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa cattctattg
    95221 ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt tatggaaaga
    95281 attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt tcgtctgcca
    95341 agtcgatcat actgacgcct cttattcaaa acgctactcc ataaaaatcc tgaaagaaga
    95401 ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaaa ctaaagaaac
    95461 ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac gataacaacc atcctaaatc
    95521 cacaagaaat acagcagacc ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag
    95581 aagacttaac gcgtacccaa tattagtcat ttgactatta ggtacgcgtt ttgttttata
    95641 agtcatgaca aatttccttc gttgctgggt agattttccg gcattgttga tagattttat
    95701 gatacttttc tacattttgt aagagaggtt ccactgcatc tgtatattgg ataaaattct
    95761 gacaacaact ttcaggtgtg tcgaaccact ctaatcttta aagataaata ttaaaataac
    95821 gattaataat attaacagtc tttatttttc accaccaaaa acctatcttt gcatatacta
    95881 aatagacgta tatacacaga taggtttgtc ttgtgaaaaa taatgattat ccttaccaaa
    95941 aaatgtttgt ttttctacat gttcgtactt atcaaaaaat atgatcctta ttgaaattac
    96001 tggctattta tattccaacg acttcttgtg ttaatttttg ttatctcata gtaaattatg
    96061 tttcattttc acttaattta gttgtcacgt gttcgtcaat tcgtaaaaac ggtaaatata
    96121 taaacacact tgcggctata attaatattt gtaaaacagc tgctcgccag tctccggctg
    96181 ttgctaaaaa tcccgatata actggtggcg tagtccaagg aatttgcact actacatgat
    96241 taattaatcc cacagcagtt gcaaaatatg ctactgtcaa agaaaaaatc ggtgcaagca
    96301 caaagggaat aatcagactg atattaaata caataggcaa tccaaaaata ataggttcgc
    96361 ttatattgaa tatagcggga gttaccgata atttagcaaa gtccttataa tcttttctac
    96421 gactgaagat aaatattgca atcaataaag caatcgtatt tcccgatcca cccattaccg
    96481 caaaggtatc tttaaaggcc atattgataa tatgtggaat ttgctctttg tttgcataag
    96541 ctgccatatt ttcttgcata ttctgaatca aaaacggatc taacaatgaa cctgatatta
    96601 ctgcttgatg gataccaaat ccaaaaaata gattagcaat agatgtaatt aacaaaaatc
    96661 ctggtaaacc agttgtaatt ttacttaatg gctctgtaac tatcgtggta attagcttat
    96721 ttatatccat attaaacaat tgaatgatta taaatgacaa taaagcaaac gaggtaatcg
    96781 taataatgat tgggattaat acattaaaag actttaaaac agcgggagga atatttccgg
    96841 atagctgaat ctgtagtttc ttattattag aaagtttaat aaataattct gtagccgcta
    96901 atccaacaaa tatacctaca aacattcctg aagctcctgt ataggatata ggtatcacac
    96961 ccgtcacttc aacagcttta tccgcatttt caggagtaaa catcatctgc ataggtgtta
    97021 atacaacaaa tgtcgaaata gcaactaata ccgctgcaat atgatttgta taatttctgc
    97081 ttgcacacat atgataagat actgctgcta caactaataa tgtagttatg cttaatgtac
    97141 cattggcaat tgaagtacca atttgttgta acgtggtcaa tgtactagta tcgacaattt
    97201 cccccatgaa tcctgttggt tctaagatca ctgaattaat aagtgtcaca aatcctgcta
    97261 acatcataaa tggcataaat gtagcaaacg catctcgcaa agttcttaaa tggatctgat
    97321 tccccaactt agtcgcaact ataccaaatt tttcaaatac aatgtccttc ctgctacttt
    97381 tgcccatatt ttttctcctt tatgcttatt gtaatcgatt actttttcca cccaaataat
    97441 acatcactta tcaaaaaaag taaatacttt taattaaata tatatatctt ttttagtata
    97501 aaaaaataag ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca
    97561 aaaacaaagt cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat
    97621 agagcaacaa ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt
    97681 gcgtcaaatt cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga
    97741 cccagttcat ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct
    97801 ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa
    97861 cggcttcgta caatattata tacatagtct tttacactga tacttaattt atcagctact
    97921 tttttcgatg gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca
    97981 accacgctaa acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg
    98041 acgacttcag tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct
    98101 acgcctttct ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta
    98161 ggaagtttct caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa
    98221 atttccatat acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca
    98281 tctatacttt aacataaata aaccgataaa atccacatca aaattataca cacaataaaa
    98341 aagatatata tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag
    98401 tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt
    98461 tttggtgggg ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag
    98521 cagagactat ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg
    98581 attcctctat tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa
    98641 aaactgggca taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg
    98701 gtaccggaaa agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga
    98761 ataataacgg aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc
    98821 aagataaagg aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa
    98881 cttgttttaa agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg
    98941 ggcctatttt aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac
    99001 gcggagcgca agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta
    99061 aagaactagc tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc
    99121 gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga
    99181 gctttttaga tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag
    99241 aatataatca gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata
    99301 ccgctcaaat tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta
    99361 tggtggatac atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg
    99421 gacgacgaat gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt
    99481 gcatggatat caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg
    99541 gcgttgcgga tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga
    99601 ttgagtatat caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca
    99661 ttaaggggta tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa
    99721 accgatatgg gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg
    99781 gcgaatttta caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag
    99841 ttgaataata ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga
    99901 aataccaata acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact
    99961 gtgacaaatg tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt
   100021 taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa
   100081 atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
   100141 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
   100201 tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat
   100261 ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg
   100321 gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc
   100381 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
   100441 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
   100501 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
   100561 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
   100621 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
   100681 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
   100741 actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt
   100801 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
   100861 attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt
   100921 cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat
   100981 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
   101041 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
   101101 aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt
   101161 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
   101221 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
   101281 acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
   101341 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
   101401 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
   101461 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
   101521 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt
   101581 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
   101641 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
   101701 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
   101761 tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt
   101821 ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa
   101881 gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca
   101941 agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt
   102001 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca
   102061 acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct
   102121 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
   102181 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
   102241 gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
   102301 ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct
   102361 atttgttcca cttttattca tcataattat tctcttactt aaagtaaact atttgcactt
   102421 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc
   102481 aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt
   102541 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
   102601 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga
   102661 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
   102721 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
   102781 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
   102841 tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc
   102901 caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg
   102961 ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga
   103021 ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
   103081 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
   103141 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg
   103201 cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt
   103261 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
   103321 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
   103381 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
   103441 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
   103501 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
   103561 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
   103621 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
   103681 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
   103741 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
   103801 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
   103861 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
   103921 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
   103981 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
   104041 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
   104101 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accgaagatc atataaacga
   104161 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
   104221 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
   104281 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
   104341 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
   104401 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
   104461 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
   104521 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
   104581 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
   104641 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
   104701 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
   104761 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
   104821 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
   104881 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
   104941 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
   105001 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
   105061 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
   105121 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
   105181 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
   105241 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
   105301 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
   105361 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
   105421 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
   105481 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
   105541 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
   105601 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
   105661 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
   105721 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
   105781 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
   105841 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
   105901 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
   105961 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
   106021 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
   106081 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
   106141 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
   106201 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
   106261 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
   106321 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
   106381 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
   106441 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
   106501 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
   106561 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
   106621 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
   106681 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
   106741 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
   106801 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
   106861 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
   106921 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
   106981 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
   107041 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
   107101 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
   107161 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
   107221 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
   107281 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
   107341 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
   107401 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
   107461 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
   107521 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
   107581 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
   107641 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
   107701 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
   107761 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
   107821 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
   107881 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
   107941 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
   108001 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
   108061 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
   108121 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
   108181 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
   108241 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
   108301 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
   108361 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
   108421 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
   108481 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
   108541 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
   108601 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
   108661 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
   108721 aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag
   108781 atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat
   108841 cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat
   108901 ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg
   108961 tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat
   109021 ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg
   109081 tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat
   109141 tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg
   109201 atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta
   109261 atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt
   109321 ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat
   109381 catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat
   109441 ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt
   109501 ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac
   109561 gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga
   109621 taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca
   109681 acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta
   109741 ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt
   109801 cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac
   109861 caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag
   109921 gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta
   109981 cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta
   110041 ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta
   110101 aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata
   110161 gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat
   110221 actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca
   110281 attcatctag aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa
   110341 aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt
   110401 ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt
   110461 tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa
   110521 aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac
   110581 accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac
   110641 tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat
   110701 aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat
   110761 tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg
   110821 tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg
   110881 tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc
   110941 tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc
   111001 tagtacatac cataatatga ttccaacaac taatgtagta acaaaaagga atcagcataa
   111061 ttgattttag actaataact tgcatcggta cttttacttt tttcaatgca ttagctaaat
   111121 agccacaagc taatccagca accattcctc caataaatcc cataccaaga ttttgacaca
   111181 tcatcccagt gataaaagct ggtgctagac cgggacggtc agcaattgaa tatgcgatat
   111241 aggctgaaat catcggtacg ataaactgta atcccgtctg accaatcatt cgcaggactc
   111301 cccagaatgt atccgtctgg tcccaaacac ctgtgccacc aaaaatgact gcaatagaag
   111361 ttagtaaccc tgctgctacg acaagcggga tcatatagga aaccccactt tggaaatgat
   111421 ttagaatttc tcttccttgt ttcttgaatt ccattagtaa ctcccccacg atatattttt
   111481 tatttgttaa ttaatgcaat tgcttcatca atcgtctctt caccatcttt tactacctct
   111541 gaggtcccta ctgttaaaat cggtttaccg tcaaagcgac ctcgttctga aacatttgta
   111601 tctactgcaa atatgacaac atctgcctcg gaaacatccg aaattgaaag tttgttttct
   111661 atccccatag ccccctgtgt ttctacttta atatctaacc ctttcttctt cgcaaacttt
   111721 tctaaattag cttttgccat ataagtatgg gcaatccctg cgatacatgc tgttactgct
   111781 actatttttg tcatatgaaa ttcctccttt taaaatgata gagcttttac caattctttt
   111841 ttatttctcg aatcttttat tcttttaaca aactcgtcat ccatcaatct tccagataat
   111901 tctgctaaaa tacgtaaatg attagtacct ttatcagtgt cttttattgc taacaaaaag
   111961 acgagatcaa ctggttgatc atctaatgcg ccccattcaa cagaccgctt taacttggct
   112021 acagccactg ttgattcaat tactgctagg cttttgccat gaggaatggc gatatttttc
   112081 ccaattcctg tggtcatatg gttttctctg tcaaatacgt ctttttgaaa ttgtttgtgg
   112141 tcagacacaa acccattagt ctctaacaat ttcgataatt tttcgactac acttttttta
   112201 tcagatgcat ttaaatctat cactatatta tttatattga ttttttcact gatcatttat
   112261 aagccttctt tcaatatata aactaatttt tcaaacgacg aactcgtttt taaatttgct
   112321 aataactcat gattcgaagc gatttcattg aatgtttcat aaattttatt caattcaggt
   112381 tttacttttt cattaagtgc aaaaaagaaa acagaatgaa caagtgtccc atcccaatca
   112441 attccttctg gattaacgta gacacagatt actggctttt ttataaaatc tacttcgata
   112501 tgcggcatag caaaatcatt catcgctgtt gaagatagtt tttctctttt gattgctccc
   112561 tctagtatcc cttctttcgc gtaattttct tttaccaatt ttttggtaat tatatttaaa
   112621 acgttcactt gattatcctt tatttggtgg gtaaaaaagc aaaatcgttt atctaataaa
   112681 tttagaaaag catcttctgt ttgttcattt aactgtagat actgttttac ttttctgaca
   112741 tctgccccta acatcaaagg actaacaaca ataacaggaa aatttgtatt ttcaatcgga
   112801 atcgtgctta taacaagttc atcagtgact tttctatctt gtagttcatt gatgcttagt
   112861 acatcttcta tctcaaccat atctgcaaaa ccgttattta ttctttgctc taataatttt
   112921 gatgttccat atccgctact acaaactaat atcactttcg ttttgtcagg tttgtaacga
   112981 tccaaaagtg attgaatatg gagtgttacg taagcgattt catcttcatt aaaatgtata
   113041 cagaatctct cttcgatttc ctctaataaa tctacactta tcataaatga ccttttaaaa
   113101 gaatatttga ttttgtccgt ataagggtta tagatattta ctcccaattt gagtctttta
   113161 actgctgcat tgagatgtaa tactagatta ttgatcaatt cttcatcagg ataaaattca
   113221 tttaatttaa ttcttactaa ctcttgtagc tcaacaccca tatctaaata ggaaagcttc
   113281 gtttgatcta agtcgtgatt catagcattt ttttcaatcg aagtaatgtg aatgtcaaca
   113341 taagcttgtt catctattgg cacaataatt gaaaatgctt tttcaatcat tttgattaaa
   113401 ataagtgtat tctttgagat ttcttcttca gaattattac tacgtgataa gaaattgctt
   113461 gtttcaatcc tttctatcgc aatagcaagg tggatagcta aagtttgaaa ttcataatct
   113521 gtaaatttca attgtgtctt attcatgtat cgattaagca attcggctac ttttttcaat
   113581 atgtccttat ttaagaaatt attcgcttca ccaatgaaat tgatattcaa gtctaagaga
   113641 tctgttttac tgttagatgc tgtaaagcca ccccaataat agttcacaac ttctgacatc
   113701 aaccttcttt tacttttttc atcagctttg atgcgcacgc catttcgatc tgaaactaag
   113761 gacatcccat gcttgtttag tctttttcta acatctttca ttgtgctttc taccgtcgac
   113821 ctgctcacgt ataattcgtc agcaagattt tgaatcttta gagaatcatt agtattcaat
   113881 aatttagcaa acacataagt tagacggtcc tccggagagt ctgcgatatt gattttatga
   113941 ctggataata aatttcgaag tgtttctttt tttccaataa gcgaaacgcc ttttttaggt
   114001 tgaaccaaca tttgtacatc tggcattttt tggattattt ctttcaaaat taaaatatct
   114061 ttgctaattg ttttcgtaga tacatataat tgttctgata attctttgta agttgtgtgc
   114121 tcattttcaa tcaaaaagta tagtattttc atttgccgtt cgctaaattc gatgcccatt
   114181 atcttcacct cctgaataca gcttaagtgt acaattatta tctctatttt catacagaag
   114241 aaagttcttg attaatgtag aagtttctaa tattcttgat tatttcaaat caataaaaaa
   114301 gcctgcaata tttatatcac aggctttttt ctctattaaa tatatataag ataatacatt
   114361 tttctactag ttctcactaa tcttgtataa ttaaaccctc cgcaacttga cggagatgga
   114421 gaagatgccc ccgtctaatc tagcggtcat ctctcctccc attccttcca ccaatttttt
   114481 tgacaggtaa agcccgattc cgctagattc cgtatttgtg cggctggtat tttcggtata
   114541 aaaacgctgg aagacttttt caacagaggt ttgcgttcct ggtttgatag gattggccac
   114601 tgttaatcga atgaatggat cctcttttga aaatgtcacc tttgcttgtc catctgcata
   114661 acgcaataaa ttctgtatga tattttgcag cactcgcagc aacatgagcg gatctgctaa
   114721 tacagttgta tttatcgagt gttcagcgaa cgctacctcg atatttttct cagaaaattg
   114781 ttcataaaaa gagaaaaata cttcttccac ctctttagaa aaagaaagcg tatggagatc
   114841 catttctttt tgcttcgtct ctattcgagc aagatcataa aactgatccg tcatactgag
   114901 taatcttgca acagattgtt tgatgatcgt taaatacttc tcccgtttct ccgtatcctc
   114961 tacctgttcc agtaattgta cataaccgtt aatagcagtc aaaggagttc tgagatcatg
   115021 agaaaggccg acgatcgata gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt
   115081 ttgttcattt tcatccaacc atgtattcag tgcatctact aggtccatta gattcttttc
   115141 tcgaaattcg actgaaagac gaccaccata ttcttgtcgt tcagaaagag tggcgatttt
   115201 cttagttaat ctcttgattt ccctttgcag gaagaaaata aaacttccca taaagataac
   115261 aacgagaagc aacagccatt taatcaaatc taaaccccac tccccacacc gtatgaatat
   115321 acacagcatg tgcctttgct agcttccctc gaagattgga aatatgcata ttcactgttt
   115381 tctcactatc aaaatatggt tcttgccaaa ctgtttcata aagatttgct cgcgaaaata
   115441 cttttttcgg atgttctaaa aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga
   115501 cagccacacc attcacattt acatccctcg actctttgtc cagtaagatt tccttgtaat
   115561 agatgcgttg cgacagatct tttacttgga cagaagtctg atgacgaagt tggatgccga
   115621 ttcgtaacaa aagttcttcg atatcaaatg gtttcgtcac atagtcatcc gctcctgctg
   115681 ccaacaagga aactttatct ctttgatcgc tttttgctga aagaacgatg atcggaacat
   115741 tcgatgtctg tcggatttct gctaacaatg cttcacctga aagccctgga agcatgagat
   115801 ctagaaggat caaatcgaca ggatatgtgc ttaacaataa ttttccttcc gtacctgaat
   115861 aagcatcaag aagtttgtat ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt
   115921 catcgtcttc gataatcaaa atagtttcca gcaggaccac ctacttaatg tcagattttt
   115981 ggaaaataaa cattcctaga gctgatgata ccacgattgt tactagactg accagaatca
   116041 ttttcggtgc ttgcgaataa gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt
   116101 ccgtaaacca ctccgttaga aattccaagc ctttgatttt tgcgaaaaca ccgggaatga
   116161 aggccgttcc catgatataa agaaaagaaa gaagcaaact gacactggta cttctagtga
   116221 tgaatgtcaa agccactgga acagataatg tccctaatgt cagcagaagc aatacaggaa
   116281 atacttttag aacataattt ccatagtttt tgattccctc aacttcacca aatccgaaag
   116341 caagtgtgta agcaagcatg gctagtcccg tgatcaaaac cacgccaagt gttgtgatca
   116401 aaagcgtaac gagaagtttc gacacataat aagtcattcg attccgtcct aaagataacg
   116461 tattgcgaat cgtaccgttt tgaaattctt ccccaatata aaagcacgca aatgctgcga
   116521 aagccagaag aatccatgca gccatttttt ctaccattaa aatccctgtt gcaccgttga
   116581 tgactgtatc cgttgcttgg tttaattcat tgccgtattt tgtatgcata taccattcgt
   116641 tccaagaata aaaaagcgga agtacgacca tcagtcctag tgagacccac attttacggc
   116701 tcttccatac ttttattttt tcagcagtta atagattaaa catgtggatt gcctccagtt
   116761 aattgtaaga aataatcttc cagtttttgc ccaactaggt gaataccatc cacaggaact
   116821 tttgcttcta ccaatgcttg attgatggct gctacttggt caattccttc aaaaagctgg
   116881 ataacacggc tagattgtac aaaataatcc ctataaccaa gagagtcgag aaccgttact
   116941 gcttgttccg tcgccgaagt gtcaattttt ataaattgac gagattcttg tgccaattct
   117001 tctttcgata attctttgat caagtgtcct tcatgcaaaa taccatagtg agtggcgatc
   117061 tgtgacaatt catctagcaa gtggctagaa agcaagacgg tgatgccccg ttcttttacc
   117121 aaacgttgga tgatttctcg catttctgcc atccctgctg gatccatccc attcgtcggt
   117181 tcatccaaaa tcaaaaattc tggattcgtg atcagtgcat ttgcaatagc taaacgctgg
   117241 cgcataccta aagagaaatt ctttgctttc tttttccctg tattttccaa tcgcatcatt
   117301 ttcagcaggt cttcgatttc tctatccgac acaccgccgt tcgctgcttg gatacgtagg
   117361 ttatctcttg cagttaattc tggataaaga gcaggggttt caatcgattg acccatacgt
   117421 cgtcttgctg cctgcaaatc cgtcgttcca aataattcga tcgaaccttc atcaatcgag
   117481 atcaaaccca taatagttct cataaaggta gacttacccg ctccattttc cccaatcaat
   117541 ccatagatca ttccacgttt gatttcgatt gatactttat ctaatgcttt ggcagcaccg
   117601 tattttttgg ttataccagt tgctttcaag accgtttcat ttccaaaatc catacttatc
   117661 cttctttctt attgatgagt gaagtatagt tgacgggtat aaagaaagca gccagaaatg
   117721 tataaagaaa gtgtaaattt tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt
   117781 ctttcctatt atcaggcagt ttgttatacg cttgtatcag tttcctgatt ctttcttttc
   117841 gaccaaagtc gtttctctgt attcctcttc actgaaaatt tttgcttctt tcagatcaag
   117901 cttgagtaat tttaccagac cggttataag gataactata gcaagactcc ctcccagaaa
   117961 aattagactg tctcgcagac gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag
   118021 tgctaatcca agtggactgg ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc
   118081 ttgtatatgc gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat
   118141 taatccttaa aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct
   118201 accaaataag aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt
   118261 tctcctgcca actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt
   118321 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa
   118381 ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta
   118441 tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt
   118501 gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt
   118561 acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga
   118621 agctttacgg gctttcttaa gacctggttt tttcttcgta tcattgaata agtttgaaaa
   118681 accttgattt ataaaggttt tcccgtaaat atattttatt atatttgatt aaatttgaaa
   118741 tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt ttagattaaa
   118801 tttttaggct cattcattct caaatccatt ctattcagtc aataaaaatt ttaaaaagac
   118861 tttataaaca aagatgttca agcgagcatc ttattttttt gtgcttaatt tgaaaggaga
   118921 acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt tgagtttggc
   118981 tgttcaagca attgttggag caattgctcc tacgattact tttgctgatg aaattaccca
   119041 tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg aaggaacttt
   119101 tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt acaacggctt
   119161 taaacaagat attttctgta ttgaaccagg agtcccgatt tacaatgagt tcactcctgg
   119221 ctatgagaaa aacccgttac cagatatgtc cgataaagcc aaattagttt ccgttttatg
   119281 gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga tctggcaaga
   119341 agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg taaatatcgc
   119401 agccattgag gcaaaaataa atcaagccat tgacgattat cagaagaaac caaccttcca
   119461 taatagtacg gctaaaacag tattagggca atcgactact gtgacggata cgaataatct
   119521 gaatttgtca gagttcgatg aggttgtgga aaatgccgca aacattgatt atcgtgtgaa
   119581 tggtaatcag ttgataatca ccccaaatgc caattcaaaa gaaagtggtg tgctgacgct
   119641 taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc aacagacttt
   119701 aatggccggg gcgattgata aacctaatac ctacacaatc aaaattgatg tggaaacgga
   119761 gggttcttta aagatcaaaa aagtcgataa agaatcgggt gatgttgtac caggaacggt
   119821 tttccattta gactttggaa aaactttacc tgcaaaagac gtgactactg acaaagaagg
   119881 cattgctaca ttggatgaga ttcctcatgg tacaaaagta accattaccg aaaaatcagt
   119941 accggcgcct tatacgattg ataccacccc catgactacc accattaaag caggcgaaac
   120001 catttacgtg acttcaaaaa acgcacggga aaaagggcaa atcattctgg ataaaagcgg
   120061 agttgaaaca gggagtgatc tttggaatga caattattcc ttagctggta atacgtttgc
   120121 cattcgcaaa gacagtccaa ccggtgaaat cgttcaagaa atgaccacgg atgaaaacgg
   120181 tcatgcggaa acaccaaaag agattgctaa tgcgttggaa ttgggaacct attatgtgac
   120241 cgaaactaaa gctagtcatg ggttcgtgaa tacgttcaaa cctgtaaaag tcgaattgaa
   120301 gtatgccaat caaaccgtag cccctgttac cagtaacgta aaagggcaaa atcaagaagt
   120361 cactggcgaa accactttga cgaaagaaga caaagatact ggggataaag cacaaggtaa
   120421 ggcagtcttt gagggtgctg aatacacctt gtttactgct aaggatggaa aagctgttaa
   120481 atggagtgag gcttttaaac cagagttagc gaagggaacg aaaggttctg atgaaacggt
   120541 cactttgact ttagacgaaa agaaccaagt tgccgttaaa catttagcta tcaacgagta
   120601 ttattgccaa gaaacaaaag cgcccgaagg atatagcctt gatgaaacaa agtatcctgt
   120661 atccatcaaa aaggtggatg atagcgaaaa aaatgccgtg attacccgaa atgttacggc
   120721 aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat cagctgcggg
   120781 cactaccgaa actggcttca atgatctgac ctttaaagtg tcgccattgg aagggaccaa
   120841 tgaaatcaca ggtgccgaag atgaagcgac cacagcttat aacgagcaat tagggtttga
   120901 tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaagc
   120961 cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta aggaaaacaa
   121021 agaagacttt gtgaagagtg agtatgtctt taccattacc gaacaagacc aaaaacaacc
   121081 aattaaaacg gtgaccgttc cttacgagaa gctgacgaac aaagcatttt ctgttagttt
   121141 gaaccgttta atgctctatg atttgcctga ggaagaagat agtttaactt ctcttgcgac
   121201 ttggaaagac ggaaacaaag aattaacttc tctggattct accgagctag ttgataaatt
   121261 gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt
   121321 ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg tgattgcaga
   121381 aacaagcgca acgttggcga acaaagagaa aacaggaact tggaaaattc agcataaatt
   121441 aaccgccgaa caagttttaa ataaaactat tgtcttattc aactatgtgt atgaaaataa
   121501 ggaagctttt gaagcaggcg ataagccagt agcgaaggat gttagcttga ataaccaagc
   121561 ccaaactgtc agttgtacag tggaacacca tgtttctatc caaacaaagg cccacctaga
   121621 aaacggttct cagaccttta ctcatggtga tgtggtggat atgtttgatg atgtgtcgat
   121681 tactcatgat gtactggatg gctcaaaaga agctttcgaa acaattctgt atgctttatt
   121741 accggatggt acaaacaaag aaatttggaa atctggcaag attgattatg aagtaaatga
   121801 taaagaattc accaaaactg tgcttgcgaa aaaagtagat accggaaaat atccagaagg
   121861 aaccaagttt acttttgcgg aaatcaatta cgataaagat gggaatatca atggaaaaca
   121921 caatgaagat ttgaaagaaa aatctcaaac cttaacacca aaagaagttc caaccatact
   121981 aagtacgccg aaacaaccgg aaacaccaac tgttccaagt ggctctcaag aatctagtcc
   122041 cacagtgaag accttccctc aaaccgggga gaaaaattcc aatgttttac tgttcattgg
   122101 ttttactttg atttttgcga cggcgggcta ttatttctgg aatcgccgga actaaggtga
   122161 tgagatgata caagtaaatc ctaagaacca acgtgcgcga ccgcctccta aaaaagtagt
   122221 cgcgctattt ttgtatccaa aactaacaat gaaaagagga aaagaagatg gaattgaaat
   122281 ttgtcgtgcc agatatggct gaaacctttg gaaagttaag ttatgccggt gaaggagaaa
   122341 ttataacaga aggatatgga cggaatacta cggtgattgg tcgcagttat catttgtatt
   122401 ccagtaagca acgggccgat gatattgagg tggtagtagc tgcggaagcc ggtgaaaagg
   122461 attttgatca agaccaaccg ctaaaagccg tgaatcccca tttggttgcc aaaggctatg
   122521 agattgagaa tcgtgggttt actgattacg tgttgtatgt tgatgattta gtgaaagcat
   122581 aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt agattcaggt ttaacatttg
   122641 ggaagttgcg tttttccgca ttacgccgag aagtacggaa gcaaaatgag gatggaacaa
   122701 ttggtaatga ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa gggcgaatga
   122761 ttcaagttag tatcccagct aatgtaccct tacgcgagtt tgcttatgat gcagaggtag
   122821 agttggtcaa tccaatcgtg gataccgttg ccaattatgt ttttcgagaa ggaactaccg
   122881 ttaattggtt cattaaggcg gatgatttag tcttgaagcg gcagccgaat caaggaaatc
   122941 ctacgaacca aaatgaaaga aagaaatagg tgaagattat ggaaattaat gttgaacata
   123001 ttttagattg tctcgatcaa tatggaaaag gcgagctgac tgaggaacaa ctcgttaaag
   123061 cattaacctc tgatgaaaaa atgtttctga ttatacatca aggtttactt ggagaaaata
   123121 gcgacagtga agaagactct gatgtactag actggttagg agaagaagag tctttcttta
   123181 tagttgtcga agtaaatgaa aacttatgtc ggcaggcaga atccattttg gaagaaattg
   123241 gtgtggagat gccggatgct attgaaggct ttttaaatca attggttgaa acgaaacaac
   123301 tcccctcctc gtagcagtaa atgactagca taactttttg aaattgccta atgaaatcct
   123361 attgggcttt ttcgattgga ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc
   123421 gcaggagatc agcacttggt ttatcacttt gttattggct ggctactcgc tctgttcatc
   123481 ggttggatga gcgtctttta ttttcaagag tttagacaat ttgatatttc taagctatca
   123541 ctttctacta ttgaaatcgt ttggtctata aaagatttag tatgtctgct tggaagtctc
   123601 gctttttcag gagctatgat attgctttat attcactttt ttcttgatca ttggcgaagc
   123661 ttgtggcatc gacaaaagct agctcggatg attctagaga accattggta tgaagtgaaa
   123721 caaactcaaa gtgaaggctt tttcaaagat ttgaatagta gccgaaccag agagactatc
   123781 agttacttcc ccaaaatctg ttatcgaatg aaagatggat tactgtctat tcgcgttcaa
   123841 atttcacttg ggaaatacca agatcagctt ttaaaattgg aaaagaagtt agaaagcgga
   123901 ctgtattgtg agttagtaga aaaggaactc aaagactcct atgtggagta tactttactg
   123961 tatgatatga tcgccaatcg aattgggata gacgaagtag tggcagaaaa tgggactctg
   124021 cgattgatga aaaatcaagt atgggcttat gattctttac cccatatgtt aatcgctggt
   124081 ggcacgggtg gagggaagac ctatttcctc ctcaccatta tcgaagcact attgaagtca
   124141 gatgcagaat tgtttatcct cgatcccaaa aatgcagatt tagctgattt aggtacggtg
   124201 atgcctcatg tttattcaca aaaggaagaa atttctgctt gtgtggaaga tttctatgaa
   124261 cgtatgatgg ctcgtagcaa ggcaatgaaa gaaatgtcta actacaaaac aggagagaat
   124321 tatgcctatc ttggacttcc acccaacttt ttaatctttg atgaatacgt ggcttatatg
   124381 gagatgttaa cgacgaaaga aagtgcggtg attttgaata agctcaaaca aatcgtgatg
   124441 ttgggtcgtc aatctggttt ctttctgatt ttggcttgcc aaaggccgga tgcgaaatat
   124501 ttaggagacg gaatccgcga tcaattcaac tttcgcgtgg ctttgggtcg tatgagtgaa
   124561 ctcggatatt ccatgatgtt tggggaggtc gaaaaaaact tctttatgaa gcacatcaaa
   124621 ggtcgaggtt atgtggatac aggtggcagt gtgattagtg agttttatac gccacttgta
   124681 ccaaaagggt atgatttctt agattcaatt agtaaattag aaaagatgaa tgattgagtg
   124741 atcttcaata ctgactttat acttcaagta acgtataata cttttatata gtaggaagaa
   124801 aggggataat taatatggaa agtaatgatt cttatttaga gtatgcaaaa gcaaataagg
   124861 ataagtttat ttcttatatt atcaaaggga aatcccccga agttggacga gacgcagttt
   124921 ttatggcagg gagtcctggg gctggaaaat cagaggttgc cattggatta gctgagaatt
   124981 atgataacca tgtgattatt gatgcggatt actttcgcac ccagtttcct gattataatg
   125041 gaaaaaactc tagtctcttt caaaaagcat cttcttggtt ggtagagcag gcatttagat
   125101 atgtcttaga tcatggctat tcttttattc tggatgcaac ttttgcaata ttaagtgctg
   125161 aaaaaaatat ctcgcgggcg ttgaaaaaac attttcgagt gacgattttc tatgtttatc
   125221 aagaccctaa aatcgcttgg aaatttacac aagaacgtga aattgcagag ggacgtcatg
   125281 tgccgaaaaa ggcatttatt aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa
   125341 agagtaaaca tccagatgta ttgcttcata tcataataaa agattatacg aataatattt
   125401 ccgaagttca ttatgctacg gataatctag aattattttt accactaggg tatagtagga
   125461 aagatttgga ggttcaatta catgactgag aaagaacaag taacgaaaat tgtaaagaaa
   125521 tacaataaga gcattgcgga tttatcggaa aatgctactg caaaagagtt caaaacggtg
   125581 attaaatatg tagccgatca ggccaatgaa aaacaacgga aattagttgg attaaataaa
   125641 aaatagtagg acaaattaat aatgtatgag ccaagagcaa aatgttcttg gcttttttct
   125701 ttgcaatgaa tgaggtaatg tatgagattt ttagatttat tcgccggtat tggtggcttt
   125761 cgtttaggta tggaacaggc gagccatcat tgtatcggtt tttgtgaaat tgataaattt
   125821 gctagacgta gttataaagc aattcatgat acaagtaagg aggtggaaat gcatgacatc
   125881 acaagtgtat cagatgagtt tattcaatcc ctcggaccag tggacatcct ttgcggcgga
   125941 tttccgtgcc aagctttttc aattgcggga aagcggcaaa gattttccga tactcgaggt
   126001 actttattct ttgaaatcgc tcggttcacc actcttctcc gacctaagct tttattcctt
   126061 gagaacgtcc gaggattgct caatcacgaa ggaggggcta cgttcgagac gatcctccga
   126121 acgctggatg gattggggta tgacgtggag tggtaggtgc ttaactcaaa ggcctacatt
   126181 ccccaaagcc gggagcggat attccttatc ggacattctc gagacgcatg taccgaacaa
   126241 gtatttccta tcattggacc gtcttcaaca tctgatcaaa acatcagaaa tttggtaaat
   126301 atcaatcctt ccaatcgagg aatgggtgga caagtctatg gttcagatgg cgtagcgcca
   126361 accttaacag gtgatgaagg aatcaaaatc gcactgccag caagggatgg aatctctgta
   126421 gcaggactat taccgggaaa gtttgaacaa ggtaatcgag tttatgaagt tactggaacc
   126481 gcccccacct tgtcaacgaa acaaggtggt acaaaaatca tgattcgaaa aaatgaaaca
   126541 gaatatcaag aggtgaagcc agaagacagt gtgaatctgg cttttcctaa ttccacaagt
   126601 cgccgaggtc gtctgggaaa acagagtgtt cataccttac taacaggaga ccaacaagcg
   126661 atcgtcacgg atcagtatca aattcggaag ctaacaccta gagagtgttg gcgacttcag
   126721 ggctttcccg attgggcttt tgatcgagat tctcaagtca attcagatag tcagttgtat
   126781 aagcaagccg gaaactctgt aacagttccg gtcatttttg atattgccag aagattaaaa
   126841 gagttggatg tacatggttt atgatgagtt gattggagaa atttattggg tgattggaaa
   126901 gattcagtca gacccagaat tagaagaaca attgcgacgg ttaaaatttg atattcgcaa
   126961 gaatggtgtc aaagtcgctg gcgatccata tttaatgaat gaggagacgg atgctcgaat
   127021 tgaaataaat caagtaattg cagaatttga gcggattgcg gaccaagcaa aagaaccaga
   127081 tattcgtcaa tacttgttag aaatgaaagc agagttggaa gtaaatggta ttacagatga
   127141 gtaacctaaa ccaacacaac tgactgttag gcgctgaccg cggaggattc gcgaagcaga
   127201 aatcctccgt ggtgctgtgc ctgttcatgg acggcgttag ccgaccatga tgtctcaccc
   127261 ccgtatctaa caggggggta caaaaacaca aaaaataagt aagcaaccat taagaatgct
   127321 tgttgtatca aagatagtaa gtaatttgta taggtgttaa ctcatttgaa aaagttaaca
   127381 ccttgaattt ggaggtgaga acttggcaca acggaattta gattatcggc tattaaaaga
   127441 ccgacgcaac gaatacggcg tttcacaaaa taaattggct acggcttgtg gacttagtcg
   127501 gccgtactta aatcagatag aaaatggtgg cgtaaccgca tctataaaaa ccatgaggaa
   127561 aatttttgac caactggaaa gctttaatcc cgatttgcct ttgacgttac tgtttgacta
   127621 tgtaaggatt cgctttccca caacggatgc acgaaaaatt attcaagaaa ttctccattt
   127681 gaaatttgag tatatgctcc atgaagatta tgccttttac tcctatcaag aacagtatgt
   127741 catgggtgat attgtggtga tgttgtctca tgaagaagat aaaggtgtgc ttttagaatt
   127801 aaaggggcga ggttgccgac aatttgaaac ttttttactc gctcaaaagc gtagctggta
   127861 cgactttttc gaggattgtc taaaggccgg tggcgtgatg aaacgcttgg acttggcaat
   127921 caatgatcga gtggggctat tggatatacc tgagctaacg aaaaaatgtc agaaggaaga
   127981 atgtatctcc ttgtttcgta cctttaaaag ttatcgttcc ggggaacttt taaaggctga
   128041 tgaaaaggac ggcatgggca ataccttata tatcggcagt ctcaagagtg aggtttattt
   128101 ctgtttgtat gaaaaagact atgaacaata tatcaagtta gggattccat tagaccaaac
   128161 tgagacgaaa aatcgttttg agattcgact gaaaaatgac cgtgcttttc atgcgattca
   128221 agatttgtta aaaggtcgta gcattgagag caccacgttt tccattatca atcgttactt
   128281 gcgatttgcg gataaagtag aaggtaaaag gcgaaccaac tggcccttga atgaacaatg
   128341 ggggcgtttt attggacgga atcggaaaga gattcaatta acttccgagc caaaaccgta
   128401 taccattgaa cgaaccttga attggctagg ccgacaagtc gcccctacgt ggaaaatggc
   128461 taaggagttg gatcgattga atcggacaac ctatatccag gatatggtac agaatgcacg
   128521 cttatcagac cgacataaaa agattttgga gcaacaaagt atggcaattg aaaatttgat
   128581 tatatgaaaa gaggaatcgt catgggaatt gtaaaagata tttttttggt atttggtggc
   128641 agtatgcttg gtgttactgt gatgtgcttg atgaatgcaa gtgcagcagc ggatcgagca
   128701 atggaaaaga aaggaaagtg acaagatgaa tttcggacaa aatttatgtg cgactcgttc
   128761 cttagtgaaa agctaaggca ctaataaaag aacgaaaaag tttggttatt aggagaactg
   128821 ataatctgac aggtggaatg atgaagtaac gctctgaaac gcctgccctg agcctccgac
   128881 aagcattgta tgaggaaact cagaatgttt gaagctcggt aaagacggct gaaagatacc
   128941 gtaatagttg atgaacatat atcttctatc gaaaactatc cagaggtggg tagcgtatcc
   129001 aacaggtcgg gggtctataa aatatctatg gttagaatgt tctataagga actgacgaaa
   129061 cagcgaatgt acgggtctaa atttgaactt tgtagaaatg caaaggtacg agaaatcgta
   129121 gctggtgtgg caaagtaaga atataggtta tgaaatgcca tataacgtta caggcggtat
   129181 caagccagca ggcttatagc tagtgaccta aggatatata tgtatagata agattatcgg
   129241 aacgaggaaa ggtattgggt tctcatcaag agaataaact gacgaagaac aataagcagt
   129301 tgaactaatg ctgaaaagca gagggcgaac ctaagaaatt cttgtaatga gaatggagga
   129361 atgccctcaa gtcgtgtata tattagaaaa gtattatgtt gtttcaataa ggattacgag
   129421 taagataaaa aagctcactc aatttcaagt aaaggagtga agccttatgc ctaaaaataa
   129481 aaatgaaaca ttgtgtgtag aagatttaag acacgctgaa tattacgaaa tgcaaaacac
   129541 ctttgatgat ttatatgcca aaagtaagaa tggagatatt ttcactcatc ttatggatat
   129601 tatcctatcg agagaaaata tattactggc atatcgaaat atcaaagcaa atgcaggcag
   129661 taaaacagca ggaacagacg gtacaataat taaagatatt ggcaaattgc cagcagaaac
   129721 agttgtcaaa aaagtaagat acattgttgc aggcagtcca catgggtatc gacctaagcc
   129781 agtgaggcga aaagaaatac caaaaccaaa tggtaaaacc agacctctag gtattccttg
   129841 tatgtgggac aggcttatac aacaatgtat aaaacaagta ttagaaccaa tctgcgaagc
   129901 taaatttagt gaaaacagct atggttttag acctaatagg tcagttgaaa atgcaataaa
   129961 agcaacctat aaccgcctac aaatcagcca gctacattat gtgattgaat ttgatattaa
   130021 gggtttcttt gacaacgtga accattccaa gctgataaaa cagatatggg ctatgggaat
   130081 acgggacaag cacttgatat tcattttaaa aaggatatta aaagctccaa tcaaaatgac
   130141 taacggtaca ataacatatc cagagaaagg aactccgcaa gggggaataa tatccccact
   130201 actagcaaac attgttctta atgagttaga ccattgggta gaaagccaat ggcaggaaaa
   130261 tcctgttaca aaaaactatg tggttcatat caataaaagc ggtagcccat gtaaaagcaa
   130321 cgcttacaaa gaaatgaaga aaacaaagtt aaaggaaatg tatatggttc gttatgcaga
   130381 tgattttaga gttttctgcc gctacaagga aagtgctgaa aaagccaaaa tagcaatcac
   130441 tcaatggatt gaacaacgat taaaattgga agtatcacaa gaaaaaacaa gaattgtaaa
   130501 tgttagaaaa agatactccg actttcttgg tttcaagatt aaaatgatac cgagaagaaa
   130561 gaagttagtt gttaaatcac atataagtga taaacaattc aaaaaccaaa aagacaagct
   130621 ggtatcacag gcaaagaaaa ttgcttctcc ctcaaaaggg aaaacggaat tagaagaaac
   130681 aagactttat aattcaatgg tattaggtat gcaaaattat tatcagattg caacgaatgt
   130741 aaatctggat tgtatgaaac tgaatagagc tgtaatggct atcttcacta acagattagg
   130801 tacaaacaaa aggggcagat tgagaaaaac aggcagacaa cttacgacaa aggaggcaac
   130861 aagatatggt aaatctaaaa tgcttcggta tgttgctggc gagggcgaac ctattttccc
   130921 tgttggatat atccaacaca gaaaaccaat ggcaaaggtg tatttagcga attgctacac
   130981 tctagaggga agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca
   131041 gttaatgaaa ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct
   131101 attttctgct caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga
   131161 aatacattgt catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct
   131221 tattttggta aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca
   131281 aaaatatata gctctattga agctaaataa agtccagctc ataagactaa ataaatatag
   131341 agtattagct ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca
   131401 atataaacac gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg
   131461 aaaagtttga gatatttata atatcaggaa cttacctatc catatatcaa tggtttttaa
   131521 ccaatgccca atccttagtc ttactggcaa ttgtggtcat tggtttgttt ttaggattta
   131581 agcgggagtt ttccaaactg attggctttt tggtgattgc tttgattgcg gtcggattgg
   131641 tctttaatgc ttctggggtg aaggatgtct tactcagttt gtttaaccga attgtcggtg
   131701 cgtaaggggg aagcacgatg gaacagatgc gtgtttatat tgcgaacctc gggaaatata
   131761 acgaaggtga attggtgggg gcgtggttta ccccacctgt cgattttgat gaagtgaaag
   131821 aacgaattgg gttaaatgac gaatacgagg aatatgcaat tcatgattat gaacttccct
   131881 ttgagattga tgagtatacg ccaattgagg aaattaatcg gttgtgtggc ttagcggagg
   131941 aacttgaagg aacgccagtt ggtgaagttg cttctgagat tcaacatgct ttcttcaatt
   132001 cttttgagga gatggtagag catgtggatg atattatcta ttatccagat tgcgatgata
   132061 tgagtgatgt agcatatttc ttgattgtag aagctggtga ttttggagaa gtaccagaac
   132121 acttgaagac gtatattgat tttgatgcgt gtggtagaga cttggagatt ggaggcaatt
   132181 atttggtaac gagtcgaggt gtttttgagt atccatatta acaaaattat tgaagggtag
   132241 ttgatttcta aactatcctt ctttttaaag gaatgatttg atgaagaaaa taaaaagcta
   132301 caccagtatt tgggcagttg aaaaggtcat ttatgcaatt aatgactttc aactgccttt
   132361 tcctgtgact tttaaccaga tggcttggtt tgttttatcc ttgctgtttg tcatcgtatt
   132421 tgcccatgtg cctccactat ctatgataga gggggccttt ttaaaatact taggtatacc
   132481 ggtggcagtt acttggttca tgtcgcaaaa aacgtttgac ggtaagaaac cattgggatt
   132541 tttacgatct tttattagct atcacttacg atttaaggtc actttcgcag gaaagaaagt
   132601 caaagaacag aagaaacgat gggacgaacc cattactctg gtaaggagtg tgaactatgt
   132661 acccgattaa gtatattgaa aataatcttg tctttaatca agagggagaa tgttttgcct
   132721 actatgagtt aataccttac aattactcct ttttgtcacc tgaacagaaa tatcaagtcc
   132781 acgataactt tcgtcaattg attgcgcaaa atcgtgaagg caagattcat gccttacaaa
   132841 ttgccacaga aagtagtatt cgtgccacgc aagaacgatc aaaaaaggaa attaccgggc
   132901 gattaacaga agtagcgaaa caacgaattg atttacagac agaggcatta gtatccatga
   132961 ttggcgatag tcagattgat tatcgctttt ttattggctt caaactgatt gcgacagatg
   133021 aagaggtcaa tctaaagaat ctcaagaaat ctttcttctc cggctttcaa gaatttgtct
   133081 atggagtgaa tcatcatttg atgggcgatt ttgtttcctt atccaatgaa gaaattcgcc
   133141 gatataccaa gcttgaaaaa ctaatggaaa gtaaattggc tcgaagattt aaagtcagac
   133201 gagtcacacc gagtgatctg atgtatttga ttgaacatat ctatggcgaa aagggtacac
   133261 catttgaaga atatgaattt cagctaccaa agaaaaagct gaagtcggaa actttggtga
   133321 aacgttacga cttattacgt cccagtcgtt gtttgattga agaaaagccc cggtacttat
   133381 gcatggaaca tgagaatcat gaatcatatg tagcgtactt aacaattaat acaattgtgg
   133441 gggaaatgga gtttccctcc tcagaacttt tctattacca gcaacaacaa tttacattcc
   133501 caattgacac tagtatgaat gtggagattg tgacgaacaa aaaggcactt gctaccgttc
   133561 gcaataagaa aaaggaatta aaagacttgg acaaccatgc ctatcaatct gacaatgaaa
   133621 caaattccaa tgtcttagat gcattggatt ccgtagatga attggaaact accttggatc
   133681 agtccaaaga atccatgtac aaactgagct atgtggttcg agtaagtgcc gaatcagtag
   133741 atgaactaaa gcgccggtgt gatgaagtgc tagattttta tgatgatacc aatgtaaaac
   133801 tagtccggcc ttttggcgat atgatggggc tacatgaaga attcttacct tctagtaagc
   133861 gatacatgaa tgattatatc cagtatgtta cgtctgattt tctcgccgga cttggttttg
   133921 gcgccactca gatgttaggg gagttggaag gcatttactt tggttataac gtagatacgg
   133981 ggcgcaatgt ttacctgaaa ccggcattgg cctcacaagg ggtcaaggat tcagttacca
   134041 atgcgctagc agcggcattc ctcggttcac tcggtggcgg aaagtccttt agtaacaatc
   134101 tcttagttta ttacgccgtc ctttttggtg gacaagctgt gattgtcgat ccaaaagggg
   134161 aacggggtgg ctggaaagaa accttgccag aaattgcgga tgaaatcaat attctcaact
   134221 taactagtaa gccaaaaaat caaggattac ttgatcctta tgtgattatg gagaaaaaga
   134281 aggattctga aagtctagca attgatattc tgaccttttt aacaggaatt tctagtcgag
   134341 atggggagaa gtttccagtg ttgcgtaagg ccattcggac ggttacccaa agcaatcaac
   134401 ggggattatt acaagtaatt aatgagcttc gaaaagaagg ctcaccagtt gcggaaaata
   134461 tcgcggatca catcgagtcc tttaccgatt atgattttgc ccatttgctt ttctcagatg
   134521 gttcgattac ccagtcgatt agtttagata ggcagttaaa tatcattcaa gtagcggatt
   134581 tggttttacc agatgcagaa accacttttg aagaatatac caccatggaa ttgcttagtg
   134641 tttccatgct aatcgtcatt tccacttttg ccttggactt tatccatagt aatcgagaaa
   134701 tcttcaaaat tgtagactta gatgaggcgt ggagttttct tcaagtagcg caagggaaaa
   134761 cattatcgaa taagctcgtt cgtgctgggc gggctatgaa tgccggggtt tactttgtca
   134821 cgcaaaatag tgatgattta ctggatgaac gcttgaagaa taatatcggg ttgaagtttg
   134881 cgtttcgcag taccgatatt catgaaatca agaaaacctt ggaattcttt ggcttggatc
   134941 gagaagatga aagcaatcaa aaacgcctac gagatttgga aaatggccaa tgcttgatgc
   135001 aggatttgta tggtcgggta ggcgtggttc aggtacatcc agtctttgaa gaactcttcc
   135061 atgcctttga tacccgtcca cctgttcaag agagaatgga gtgaggggat gaaaaagaaa
   135121 ctttttcgga ttgtgggaat cgtttttatt agcatactca ctctgatagt tctattgagt
   135181 ttagttggta cggtagccga ggcgacaggg ctagttgatg atacagttga agcaggaaat
   135241 ctatattcca aatattcact gaacaactat caactagatt tctttgtgga tagctcttgg
   135301 gattggttgc cttggaattg gggtgatggc ttagggaaaa gcgtgatgta tggtctttat
   135361 gccattacta atttcatttg gacggtgagt ttgtatttgt cgaacgccac gggttatgtg
   135421 gttcaagagg cttacaaatt agatttcatt tcggatacgg ctgaaagtat tggaaagaat
   135481 atccaaacat tggctggtat tactgaaaat ggactccaga cctccggatt ttattttggg
   135541 tttctattat tgatgatttt agctctgggg gtgtatgtgg cgtataccgg attattaaaa
   135601 cgagaaacca cgaaagcagt tcgagccgtg attaattttg tgatgatttt tctactatcg
   135661 ggttcgttca ttgcttatgc gcctacctat atcacaaaaa tcaatgactt tagctcagat
   135721 gttagtgaag cggcccttac ccttggaacc gagatcgttg tacccaattc agaaagccaa
   135781 ggaaaagata gtgtggattt gattcgggat agtttgtttt cgattcaagt ccaacaacct
   135841 tggttactac tgcaatttga tgattccaac atagaagaaa ttggtgaaga ccgggtcaac
   135901 aagattctat ctgtgagtcc agatgaaaac aaaggcaaag atcgggaaga agcggtcaag
   135961 gcagagattg aagacaatga caatgcgaat ctcagtatta ccaagaccat gaaccgttta
   136021 gggaccgtgg tctttttggt attgttcaat attggcattt ccttctttgt ttttcttcta
   136081 acagggatta tgttgttctc gcaaattctc ttcattatct ttgcgatgtt tctgccaatt
   136141 agtttcttat tgtccatgtt gccgacttat gaaagtttag gaaagaaggc gattattcgt
   136201 ttattcaata cgattatgat gcgagcgggt gttactttgg tgattacgac tgcatttagc
   136261 atctccacga tgtttttcaa tatttcggca acctatccat ttttcatggt tgcttttctg
   136321 caaattgtga cctttgccgg tatctatttc aaattgggcg acattatgag tatgttcaac
   136381 ctccagagta atgatagcca gtcaatggga agacgcgtca tgcgaaaacc gcaaatgtta
   136441 atgaatcgaa aattgcggca actcaatcga aatgtgggac gagctttggc ttttggtggt
   136501 ggtgcagcag tcggaaataa gttggcaaag gaacaatcaa aatccaaatt taagccatcc
   136561 ggttcttctc tacgaaagaa ttcacgatta ccgaacgatc acgaaatact atctgactta
   136621 actaaggaga atccagtttc aaataataag aaacaatccc gaatgaattt ggctgggaga
   136681 aaaatcggca aagttttgga tacccaagct ttggtgaaag ataagactaa acaagtaaag
   136741 gatcaggtaa ggaatacgcc aacgaatttg aaatacaatc tccataaagg aatcgaaaag
   136801 tcgaaaaaag caccagaaga attcaagcgc ggactgttcc aagaaaaagc ggatcgagtg
   136861 gatttgcgag aaaaacaacg acaacgaaga aatgcgaaaa tggacgaaaa acgcaagata
   136921 ctgagtgaag caacaaatcg tcagggaaaa agaagggcga atattccaat agataaagca
   136981 gctcaacctc agaaagatat gatgctgaaa aaagaagcac caaagcggat tgtgagagca
   137041 aatctgaatc ccgaaatcaa acgaaaacta actagtcaag aaatcatttc aaaagggaaa
   137101 agccaatcag caagtaagaa aaattctttc caacaagtga aacagaggtc cactctgcca
   137161 aagcagacca atcaaaaagt acaaaaaaga atgaaccatc gtccaaaatc aaggtcaggt
   137221 gagaaaaaat gaacttgaag aaagtgggtg ttatttcatt aacttttctg accatctctt
   137281 ttatgggtat tttgatgtgt gtgggtttgt tgtttggtga agacagtgag ggaaatggta
   137341 gttctggcac ttctcctggc ggaactagtg tctcagaaga agtattgaaa caccgtgcaa
   137401 tggtggaaaa gtatgccaaa gaatcggata taacagagta tattccaatt cttttggcga
   137461 ttattcaagt ggaatctggt ggcacaatgg aagacgttat gcaaagttct gagtcagccg
   137521 gattaccacc taattcatta agtaccgagg catccatcaa acaaggctgt ctctattttg
   137581 cgtctttggt taagcgttcc aaagaactag ggtgtgatca agatagtatt attcaggcct
   137641 ataactatgg tggcgggtat ctagatcatg tcgggaaaaa cgggaagaag catagttttg
   137701 ctttagctga atctttttca aaggaaaaat caggtggtca aaaagtcgat taccccaacc
   137761 caatcgcgat taaggaaaat ggtgggtggc ggtataatta tggcaatatg ttttactgtt
   137821 tattagtgaa gcaataccta actacaactc agtttgatga caaaatagtc caagaaattt
   137881 ttgatgaggc atttaagtat gaaggaacgg cctatgtgtt tggtggctca agtccagaaa
   137941 cggggtttga ttgtagtggc ttgacccaat ggtgttatgc caaagttgac ctgaaacttc
   138001 ctcgggtggc tcaggatcaa tatgatgcca tgactcatat tgatctaaaa gatgccaaac
   138061 caggtgattt agttttcttt cattcaacct atgatgccgg tacttatgtg acccatgtgg
   138121 gcatttacgc aggggagaat cgcatgtttc atgcaggaga tccagtgggg tggacgaatc
   138181 taacggaaaa ctattggcgg cagcatctta tcggagctgg ccgatacaaa taagagaaaa
   138241 gaggaaaaag aatgaagata aaaattgaac ggaatcaaaa ggaaaagagc ccaaaaaaga
   138301 aaaaagaacg agttgtatcc gtgggtaagc atcgaaaaat ggtgcttgcc ctttggcttt
   138361 tattgtcttg tagtcttgct tttggcgttt ataaaaattt caccgccatt gatcaacata
   138421 caactcatga aaaagtggtt ataaaagaga aggtagtgaa tacttctggc gttgaaagtt
   138481 ttacagagga ttttgcgaaa gaatattttt catggaaaaa taacaaagaa gcaattgaaa
   138541 agagaatgag taatctcgaa cagtacctta cagaaaacgg tctcgcttta atccaagata
   138601 tggttcgagc agatattccc acgagttccg aagttcaatc tgtaaaaatt ctagatgttg
   138661 aaaaacactc ggaagaattt gtcgtttcat ttttagtgga gcaacatatt acagagggaa
   138721 aaaagacgca atcaatttct tctgcatatc gagtgacaat ctttgaagat gaaaacagga
   138781 atcatttagt aactagtttg ccaacaatga ttggaaagcc agacaaggca aaatatgaag
   138841 caaaacaagt agaaaacgat tcagaaattg atgccaaaac aacggaagaa atcaccgatt
   138901 ttttagaaac attttttaaa ctttatccat cagcttctga aaaagaattg gagtattatg
   138961 ttgaaaatgg tgcgatgcga ccaatcaata ttgacttaaa gttttcggag ttagagaatc
   139021 cagtttatct acaaaacgga aaaaattttc tagtgagtac aaagataaaa taccttgata
   139081 atgtatcaaa aaccactagc tactttcagt acaaattagt attacaaaaa aatggaaact
   139141 ggaaaatcgt tgatgttaaa taataatgac tcaatctaat tttattagaa gttataagta
   139201 gtgtaactta tttcaggcag aagtcgccca tcctctatag gtgggtgatg aatgccattc
   139261 ggtaactcga aaaccgacaa ttaagacata gtgccttttt attttaatcc atgtcataat
   139321 cagtctatat aggaaatggt tgatatacca tggaattaga tacaaataat cattcagttt
   139381 ttcttcttgg ttaccccctt attttagtag tgaaacattg taaacatgtc attgatgatg
   139441 taatgtcagc ttatgcaaaa actgcctttg aaagaatttc agaatcacat cacattacct
   139501 tagatgaatg aaatcgtgat aaagaatact ttcatattat gctcaaagca caacctaaaa
   139561 ccgaattaac aaaattcagc aatgcctaag ctcacgtctg ataaaatgag actttctaag
   139621 ggtcaaaaat ttcctttgaa tacgattatt gcttagataa aagtaaaagc cttagaacta
   139681 cggggatagc ttggttaata agagatatcg ttgttagtaa agacataggc ttgcaagtat
   139741 gctctattcc caagaatctc ccccttcaag caccaaggat cgttaaattc agctaagtgg
   139801 caagtaattc actagacgat aattatctcc atatttcaat ctcagctttt ccatttttca
   139861 tagcaaattc cgttattttt atagcatcta aaagattcag cttttccact gatctttcac
   139921 cacttttcag cttttttatt gtgcttaaat tgataccagt accttttgat attgaattgg
   139981 aagtaaaatc attaagggtt ttttttatta aattcatatc cgcaattcca gcgttcataa
   140041 gaatgaagat tctcctctca tagactactt tgaaaatggt actacagtgg ctgtttatat
   140101 atttcagtag gttactgtag tggtcatctt attataatta gaaaaggaac ctaaatcaaa
   140161 aatgctaaag aaaattttca tagtattaag cctctcatga ctaaagccac ggaattcttg
   140221 ataaaagagt atacttgcaa gcctaaacag cggtatctct tattaaccaa gctatccccg
   140281 ttgttcctaa ggttcttgat gttttctaag gcaatgcttg tattcatagc ccttggtcaa
   140341 aatattgata ctatcgttga cgtttcgatt atgatgtata cggcaaatag gacaagtcca
   140401 ttctcgaatt gtgagtgact tcttaccatc tgtgtgtcta cattctgaac caatttgact
   140461 agacggaaat catttatcta tcttaatgat tttttgacag aagtatcgaa aatctttaca
   140521 taatctgggt gatgtattct tatgcagatg agagcgttgg ctccgtattc ataaccagta
   140581 gcctctaaaa cgtagtccat cggtgggata cctagcttgc ggttgccagc ggctttcgca
   140641 gtcttcttgt gatcaaatca agcatctcat tgagcactag tattttgaag ataatacttg
   140701 agtttttcgg cttttagaag ttcacttcat cttccatcat ccatggaaat cccactcacc
   140761 catacgcagg cctccaaaaa ttcttcaaca gtggtttttt cagacatttt tctgaatacc
   140821 tccttgtttt ttgatacgcc agatagtcag gaaaaaggca acaaaacaca aaacaaagct
   140881 gcctaaaaag gtaatcttca ttccatataa aaagacatcc ggccgatttg cgatatagtt
   140941 agttacttgc tggcccatct tagcagacat cgcttgatat aagaccgtcg tagaaagcga
   141001 aatcccgctg accatcccca aatttcgggc aaaggaattc atcgagcctg ccagtcccaa
   141061 gttttcgata gtgaccgagc tcataaccat cgtattattg ggggactgga acaaggcatt
   141121 gccggcaccc atgatggcag tagcgacaat gtatagccac aaaggagagt ccactgacaa
   141181 aaacatatac atgacctggg ttaccgacaa taagcccaag cctactaata ccaaaatctc
   141241 cgggccaacc cggtcggtca ggtagccgct taaaggcgcc ccgacgacca tcatcaaagg
   141301 aaagaccatc atcaacagcc cagcatagct aggactcaac ccgcgagcat tttgcagata
   141361 aaaaggaatc actacattga caaagaagtt ggaagaaaaa atcaacaagg ctgtaatcaa
   141421 gctcatggta aaaatcttgt tcttaaaaat accgaaagaa atcaatggag aggctgcttt
   141481 cttttcaact tttaaaaagc tgacaaaggc aatcactgaa attacgaaca gtacaagggg
   141541 gactggatgg aaaaagccac tttcttgccc caaaaagatt gctccaaaaa agctgataat
   141601 cgtagccgca atggcagcaa agccccctcc atcgattgta tggccgctca tggtgatatc
   141661 ttttggtaag aatttttcag caatcaacat cgttacgatt cccaccggta cattgatcca
   141721 aaaaatgtac gcccaagaaa attgggacaa aatcaagccg ccaagacctg gtccagcaat
   141781 cgagcctaat gaaacaaacg cacctattgc tcccaatgcc cgaccacgtt catgtatggg
   141841 aaagacttcc gtgatgattc cggcatttgt agccatagtc atactagcac caatggcttg
   141901 taccacccgt gcgaacaata aaatacctag cgaatgattg aacccacaca acagcgatcc
   141961 caagacaaag atcagcgtac caatccgaaa tactttgatt ttcccacggc tatcgccaat
   142021 tttaccaaac agcaacaaac aagaacagac aatcatcaga taaacggaca ctacccattc
   142081 cgcttgattc attggtatgc ccatatcatc tgagatggta ggtagtgcaa tattgacaat
   142141 actgccatcc aatgtggaca tgaaggtaaa catagatata gcaatcaaaa tccaccagcg
   142201 atttttttgg acttttgcat cttcttgata actatacata ataggatttc ctttctaatg
   142261 atcgtgtagc taagaacaat ataacctatg tctactgccc aacaacttct cgcgactcta
   142321 gccataggaa gcttgagtaa tttcgatttg atatcctcat gtaaccatgt aagttggaga
   142381 tgctttattt gaacttactc tatagtaact agtgataatt cgctttactt tcttctagca
   142441 atttctaaaa gtcgacttca ttgttcttca gattaatagt gtgagctatc tcgtgagacg
   142501 taagcaaatt tccattctac tttttcccaa ttttttcata tttatggtgt tgcacctatt
   142561 tgttttccaa tttgcttttc aaatacattg ctatgatatt caattccata ggattatact
   142621 aaatatcaat agtatgaata atatattata gatgtttata tatcactgtt aattattatc
   142681 cacaataatt aatgattacc tcgatatttg tcaatcaaag gttgaaacaa tttattttca
   142741 gcatcttttc tagcttgagc tgcactctta atagaatcat acgatcctaa atagtacctc
   142801 ttttttttta aagttatata cgcaatctat ttctctttag aacgataaac tcccttaaca
   142861 ccacttgtat tatttttcgg taattttgac gttagacact ctattactgt cccttcaaca
   142921 catgtccttg tttcttgcct ttttaaacat ccacaacttt gagtatgtcc aaaaaataag
   142981 ttactataac ttatctcaca catactgcca cactcacaat gacaaaccca ttttgttttc
   143041 catgaacagt tattgtccaa aggatacttt gcagtaagta atccgaattt tttattttca
   143101 atgtgatgct tttttgattt caaacatcca cagcttttta catcaccttt tagaagtcgc
   143161 tttgccgata ctaacttttt tctcccacac agacattgac attcccaaac aacagttcct
   143221 tcacttcgca gtttagttgg cttgattgca actaacttac caaaagtttt atttgtcaaa
   143281 tttttatatt tatgcctatt tttataattt ccacatccac aacttttggt tctacctgtt
   143341 agtaagtttg atcgatatac aatcgtttca ttcccacagt cacataggca acgatatttt
   143401 ttatatccat tatgagtaat ctcctcaaca acagtaaggt gaccatatct ttccttcact
   143461 gcacctaccc tcccaagaaa gaagtaccat ataatttcaa ttaactttta ataaatcaga
   143521 aaaaaatatt tccaattttt cttagtctga ttttatgact aatttacaat acaagactga
   143581 acgatacgta cacgaaatcc tcaagataga taaattgttt tcatctagtg aaacctgctc
   143641 aaaatatgag cataaagacg gcaataatac actcgatatt cgataatgga cttgttttat
   143701 ttgtcaaacc catcgtaatc gagatatcaa ttccagtctc aatatcctga tcaaaggtct
   143761 aagaatccaa ccattttctt gttactttgt cagattgatt acctttttta gtctccgaat
   143821 aataaggttt tcggatcaac taacatgctc tgagaaagac tactatcttt gcaaatttcc
   143881 aaatgaaggt gtgggccagt actattccca gtacttccta catagccaat tatttgtcct
   143941 tgcaaaacat tatctccagt ttttactagg tattgttgct ggtgagcata taacgcggtt
   144001 gatccatctt cgtgttgtat caccacgtaa tttccccaag agtagtgaaa ctctgcttta
   144061 acaaccttac cagctttaac tgcatgaatc ggctcaccct gtgtagctgc catatcaagt
   144121 cctcggtgaa attcatttcc acgatttcca aacaaactgg ttattgaata attatgaata
   144181 ggcacttggt atccagtctt actcatttgt ggacctttaa cttctttatc atagttggtt
   144241 aaatcgtagg ttttaattaa ttcatttagc ttcaaatagt attgtgtatc tgttgcatac
   144301 cgtcctgtaa ggaatttagt tgcttctcga tatgttttac tattagcttt taatgctcct
   144361 gcataaaaat ctttatttcc actaattccc ttagttaata atttggcata atcttcaaag
   144421 ctttcctcat agttttcata cactctaaat tttgattggg tcgtataaag cgttccatta
   144481 cccaaatctt cgtttgttac cataatgaca aagtttccat tataagttcc ttttatccca
   144541 aataaattgt aattcggtgc ctgagctaac tggctttgac cactggcaga ttctaagatc
   144601 gcttgtgcaa tcataacaga agcatataaa tcatatttct gaccaatttt tctcgcagac
   144661 tcaccaattt tcataataaa attttctaca gattcatttt tgtcaaaatg aatttcttca
   144721 ttaccagata gtattggttg tgattggaaa gattctgttg attctatttg ctgtggtgtc
   144781 gaaaaagttt ttgtttgtcc ttccgttttt ggcgcttcct tttttgaaga ttctatatgg
   144841 tttggttttg tcactttcga agaagttatt tcacttgtac tacttgaagt tgttgaagat
   144901 tcggtacttg aaatcgtact actttcactt gacgaagttg ttgaagactc ggcatttgaa
   144961 gtcgtactac tttcacttga tgaagttgtt gaagactcgg catttgaagt cgtactactt
   145021 tcacttgatg aagttgttga agactcggca tttgaagtcg tactactttc acttgatgaa
   145081 gttgtcaaaa gttcactact ctctgctgtt acatcggaat tactcatttc agtcgtctcg
   145141 gttctttctt ttgcaatttt gtatgttgaa ttttcgcttg ccaacacatg gaaaggtgtt
   145201 gctataagga gttgggacat taatataact gctgatgcaa gttttaaaga ttttttcatt
   145261 tgatttacct ctcttttttc tctttaatag tgttttctat taaaaattat gataaatatt
   145321 ttcaatgaat tgagtaatac agtatatttc ttgatttcat ttagtaacga ttatgtttca
   145381 atgtttaatc ctgttactgc ctttaaa
//
LOCUS       DAZUMO010000002        87449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000002 DAZUMO010000000
VERSION     DAZUMO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.002"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8ER_000134"
     CDS             476..1339
                     /locus_tag="ACG8ER_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5048755.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8ER_000135"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8ER_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5048756.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8ER_000136"
     CDS             3339..3860
                     /locus_tag="ACG8ER_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5048757.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8ER_000137"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8ER_000137"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS5048758.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8ER_000138"
     CDS             5941..6759
                     /locus_tag="ACG8ER_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS5048759.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8ER_000139"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8ER_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048760.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8ER_000140"
     CDS             7228..7806
                     /locus_tag="ACG8ER_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5048761.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8ER_000141"
     CDS             7901..8038
                     /locus_tag="ACG8ER_000141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048762.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACG8ER_000142"
     CDS             complement(8851..9354)
                     /locus_tag="ACG8ER_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5048763.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACG8ER_000143"
     CDS             9713..10738
                     /locus_tag="ACG8ER_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5048764.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACG8ER_000144"
     CDS             11061..11939
                     /locus_tag="ACG8ER_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS5048765.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="ACG8ER_000145"
     CDS             12415..12531
                     /locus_tag="ACG8ER_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5048766.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACG8ER_000146"
     CDS             13459..13797
                     /locus_tag="ACG8ER_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048767.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACG8ER_000147"
     CDS             13891..14121
                     /locus_tag="ACG8ER_000147"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS5048768.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="ACG8ER_000148"
     CDS             14521..14892
                     /locus_tag="ACG8ER_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS5048769.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="ACG8ER_000149"
     CDS             complement(15166..15858)
                     /locus_tag="ACG8ER_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5048770.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="ACG8ER_000150"
     CDS             complement(15861..16646)
                     /locus_tag="ACG8ER_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5048771.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8ER_000151"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8ER_000151"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5048772.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="ACG8ER_000152"
     CDS             18308..18625
                     /locus_tag="ACG8ER_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5048773.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="ACG8ER_000153"
     CDS             18638..20311
                     /locus_tag="ACG8ER_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS5048774.1"
                     /translation="MDGIIKQIEKGKAFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="ACG8ER_000154"
     CDS             20325..21956
                     /locus_tag="ACG8ER_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS5048775.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACG8ER_000155"
     CDS             complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACG8ER_000155"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5048776.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACG8ER_000156"
     CDS             complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACG8ER_000156"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS5048777.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25190..25795
                     /locus_tag="ACG8ER_000157"
     CDS             25190..25795
                     /locus_tag="ACG8ER_000157"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5048778.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26103..27119
                     /locus_tag="ACG8ER_000158"
     CDS             26103..27119
                     /locus_tag="ACG8ER_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5048779.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27260..28561
                     /locus_tag="ACG8ER_000159"
     CDS             27260..28561
                     /locus_tag="ACG8ER_000159"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS5048780.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28607..29269
                     /gene="deoC"
                     /locus_tag="ACG8ER_000160"
     CDS             28607..29269
                     /gene="deoC"
                     /locus_tag="ACG8ER_000160"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS5048781.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29290..29688
                     /locus_tag="ACG8ER_000161"
     CDS             29290..29688
                     /locus_tag="ACG8ER_000161"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS5048782.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29754..30836
                     /locus_tag="ACG8ER_000162"
     CDS             29754..30836
                     /locus_tag="ACG8ER_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5048783.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31048..32610
                     /locus_tag="ACG8ER_000163"
     CDS             31048..32610
                     /locus_tag="ACG8ER_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048784.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32603..33736
                     /locus_tag="ACG8ER_000164"
     CDS             32603..33736
                     /locus_tag="ACG8ER_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5048785.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33736..34692
                     /locus_tag="ACG8ER_000165"
     CDS             33736..34692
                     /locus_tag="ACG8ER_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5048786.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34791..35957
                     /gene="deoB"
                     /locus_tag="ACG8ER_000166"
     CDS             34791..35957
                     /gene="deoB"
                     /locus_tag="ACG8ER_000166"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS5048787.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35981..36799
                     /locus_tag="ACG8ER_000167"
     CDS             35981..36799
                     /locus_tag="ACG8ER_000167"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5048788.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36816..37523
                     /gene="deoD"
                     /locus_tag="ACG8ER_000168"
     CDS             36816..37523
                     /gene="deoD"
                     /locus_tag="ACG8ER_000168"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5048789.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACG8ER_000169"
     CDS             complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACG8ER_000169"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5048790.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38679..39260)
                     /locus_tag="ACG8ER_000170"
     CDS             complement(38679..39260)
                     /locus_tag="ACG8ER_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5048791.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACG8ER_000171"
     CDS             complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACG8ER_000171"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS5048792.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40395..40808
                     /gene="rpsL"
                     /locus_tag="ACG8ER_000172"
     CDS             40395..40808
                     /gene="rpsL"
                     /locus_tag="ACG8ER_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS5048793.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40892..41362
                     /gene="rpsG"
                     /locus_tag="ACG8ER_000173"
     CDS             40892..41362
                     /gene="rpsG"
                     /locus_tag="ACG8ER_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS5048794.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41456..43543
                     /gene="fusA"
                     /locus_tag="ACG8ER_000174"
     CDS             41456..43543
                     /gene="fusA"
                     /locus_tag="ACG8ER_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5048795.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43715..44902
                     /gene="tuf"
                     /locus_tag="ACG8ER_000175"
     CDS             43715..44902
                     /gene="tuf"
                     /locus_tag="ACG8ER_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5048796.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACG8ER_000176"
     CDS             45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACG8ER_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS5048797.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45648..46277
                     /gene="rplC"
                     /locus_tag="ACG8ER_000177"
     CDS             45648..46277
                     /gene="rplC"
                     /locus_tag="ACG8ER_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS5048798.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46305..46928
                     /gene="rplD"
                     /locus_tag="ACG8ER_000178"
     CDS             46305..46928
                     /gene="rplD"
                     /locus_tag="ACG8ER_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS5048799.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46928..47218
                     /gene="rplW"
                     /locus_tag="ACG8ER_000179"
     CDS             46928..47218
                     /gene="rplW"
                     /locus_tag="ACG8ER_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS5048800.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47257..48090
                     /gene="rplB"
                     /locus_tag="ACG8ER_000180"
     CDS             47257..48090
                     /gene="rplB"
                     /locus_tag="ACG8ER_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS5048801.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            48133..48411
                     /gene="rpsS"
                     /locus_tag="ACG8ER_000181"
     CDS             48133..48411
                     /gene="rpsS"
                     /locus_tag="ACG8ER_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS5048802.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48434..48781
                     /gene="rplV"
                     /locus_tag="ACG8ER_000182"
     CDS             48434..48781
                     /gene="rplV"
                     /locus_tag="ACG8ER_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS5048803.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48795..49451
                     /gene="rpsC"
                     /locus_tag="ACG8ER_000183"
     CDS             48795..49451
                     /gene="rpsC"
                     /locus_tag="ACG8ER_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS5048804.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49454..49888
                     /gene="rplP"
                     /locus_tag="ACG8ER_000184"
     CDS             49454..49888
                     /gene="rplP"
                     /locus_tag="ACG8ER_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS5048805.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49878..50066
                     /gene="rpmC"
                     /locus_tag="ACG8ER_000185"
     CDS             49878..50066
                     /gene="rpmC"
                     /locus_tag="ACG8ER_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS5048806.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACG8ER_000186"
     CDS             50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACG8ER_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS5048807.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50415..50783
                     /gene="rplN"
                     /locus_tag="ACG8ER_000187"
     CDS             50415..50783
                     /gene="rplN"
                     /locus_tag="ACG8ER_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS5048808.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50820..51128
                     /gene="rplX"
                     /locus_tag="ACG8ER_000188"
     CDS             50820..51128
                     /gene="rplX"
                     /locus_tag="ACG8ER_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS5048809.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            51156..51695
                     /gene="rplE"
                     /locus_tag="ACG8ER_000189"
     CDS             51156..51695
                     /gene="rplE"
                     /locus_tag="ACG8ER_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS5048810.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51714..51899
                     /locus_tag="ACG8ER_000190"
     CDS             51714..51899
                     /locus_tag="ACG8ER_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS5048811.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51935..52333
                     /gene="rpsH"
                     /locus_tag="ACG8ER_000191"
     CDS             51935..52333
                     /gene="rpsH"
                     /locus_tag="ACG8ER_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS5048812.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52365..52901
                     /gene="rplF"
                     /locus_tag="ACG8ER_000192"
     CDS             52365..52901
                     /gene="rplF"
                     /locus_tag="ACG8ER_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS5048813.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53062..53418
                     /gene="rplR"
                     /locus_tag="ACG8ER_000193"
     CDS             53062..53418
                     /gene="rplR"
                     /locus_tag="ACG8ER_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS5048814.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53439..53939
                     /gene="rpsE"
                     /locus_tag="ACG8ER_000194"
     CDS             53439..53939
                     /gene="rpsE"
                     /locus_tag="ACG8ER_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS5048815.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53954..54133
                     /gene="rpmD"
                     /locus_tag="ACG8ER_000195"
     CDS             53954..54133
                     /gene="rpmD"
                     /locus_tag="ACG8ER_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS5048816.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            54177..54617
                     /gene="rplO"
                     /locus_tag="ACG8ER_000196"
     CDS             54177..54617
                     /gene="rplO"
                     /locus_tag="ACG8ER_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS5048817.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54617..55912
                     /gene="secY"
                     /locus_tag="ACG8ER_000197"
     CDS             54617..55912
                     /gene="secY"
                     /locus_tag="ACG8ER_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS5048818.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55974..56621
                     /locus_tag="ACG8ER_000198"
     CDS             55974..56621
                     /locus_tag="ACG8ER_000198"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS5048819.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56812..57030
                     /gene="infA"
                     /locus_tag="ACG8ER_000199"
     CDS             56812..57030
                     /gene="infA"
                     /locus_tag="ACG8ER_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS5048820.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACG8ER_000200"
     CDS             57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACG8ER_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS5048821.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            57197..57562
                     /gene="rpsM"
                     /locus_tag="ACG8ER_000201"
     CDS             57197..57562
                     /gene="rpsM"
                     /locus_tag="ACG8ER_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS5048822.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57590..57979
                     /gene="rpsK"
                     /locus_tag="ACG8ER_000202"
     CDS             57590..57979
                     /gene="rpsK"
                     /locus_tag="ACG8ER_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS5048823.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58059..58997
                     /locus_tag="ACG8ER_000203"
     CDS             58059..58997
                     /locus_tag="ACG8ER_000203"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS5048824.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59025..59405
                     /gene="rplQ"
                     /locus_tag="ACG8ER_000204"
     CDS             59025..59405
                     /gene="rplQ"
                     /locus_tag="ACG8ER_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS5048825.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59677..60516
                     /locus_tag="ACG8ER_000205"
     CDS             59677..60516
                     /locus_tag="ACG8ER_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5048826.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60492..61361
                     /locus_tag="ACG8ER_000206"
     CDS             60492..61361
                     /locus_tag="ACG8ER_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5048827.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61351..62148
                     /locus_tag="ACG8ER_000207"
     CDS             61351..62148
                     /locus_tag="ACG8ER_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5048828.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62351..63097
                     /gene="truA"
                     /locus_tag="ACG8ER_000208"
     CDS             62351..63097
                     /gene="truA"
                     /locus_tag="ACG8ER_000208"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5048829.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(63552..66221)
                     /locus_tag="ACG8ER_000209"
     CDS             complement(63552..66221)
                     /locus_tag="ACG8ER_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS5048830.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(66459..66647)
                     /gene="rpmB"
                     /locus_tag="ACG8ER_000210"
     CDS             complement(66459..66647)
                     /gene="rpmB"
                     /locus_tag="ACG8ER_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS5048831.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            66917..67279
                     /locus_tag="ACG8ER_000211"
     CDS             66917..67279
                     /locus_tag="ACG8ER_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5048832.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            67331..69013
                     /locus_tag="ACG8ER_000212"
     CDS             67331..69013
                     /locus_tag="ACG8ER_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS5048833.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            69130..71166
                     /gene="recG"
                     /locus_tag="ACG8ER_000213"
     CDS             69130..71166
                     /gene="recG"
                     /locus_tag="ACG8ER_000213"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS5048834.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            71215..72216
                     /gene="plsX"
                     /locus_tag="ACG8ER_000214"
     CDS             71215..72216
                     /gene="plsX"
                     /locus_tag="ACG8ER_000214"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS5048835.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            72338..72580
                     /gene="acpP"
                     /locus_tag="ACG8ER_000215"
     CDS             72338..72580
                     /gene="acpP"
                     /locus_tag="ACG8ER_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5048836.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            72848..73852
                     /locus_tag="ACG8ER_000216"
     CDS             72848..73852
                     /locus_tag="ACG8ER_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048837.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            73853..74797
                     /locus_tag="ACG8ER_000217"
     CDS             73853..74797
                     /locus_tag="ACG8ER_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048838.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            74797..75759
                     /gene="opp4B"
                     /locus_tag="ACG8ER_000218"
     CDS             74797..75759
                     /gene="opp4B"
                     /locus_tag="ACG8ER_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5048839.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            75786..76700
                     /locus_tag="ACG8ER_000219"
     CDS             75786..76700
                     /locus_tag="ACG8ER_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5048840.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            76726..78507
                     /locus_tag="ACG8ER_000220"
     CDS             76726..78507
                     /locus_tag="ACG8ER_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5048841.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            78855..79541
                     /gene="rnc"
                     /locus_tag="ACG8ER_000221"
     CDS             78855..79541
                     /gene="rnc"
                     /locus_tag="ACG8ER_000221"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS5048842.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            79561..83142
                     /gene="smc"
                     /locus_tag="ACG8ER_000222"
     CDS             79561..83142
                     /gene="smc"
                     /locus_tag="ACG8ER_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS5048843.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            83151..83960
                     /locus_tag="ACG8ER_000223"
     CDS             83151..83960
                     /locus_tag="ACG8ER_000223"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5048844.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            83971..84969
                     /gene="ftsY"
                     /locus_tag="ACG8ER_000224"
     CDS             83971..84969
                     /gene="ftsY"
                     /locus_tag="ACG8ER_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS5048845.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            85156..87423
                     /gene="gshAB"
                     /locus_tag="ACG8ER_000225"
     CDS             85156..87423
                     /gene="gshAB"
                     /locus_tag="ACG8ER_000225"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS5048846.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT        28222 a        15676 c        18721 g        24830 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tgtttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaagcatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg
    22501 gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg
    22561 gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt
    22621 gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt
    22681 gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc
    22741 atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc
    22801 aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt
    22861 ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg
    22921 catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt
    22981 accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc
    23041 accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt
    23101 acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt
    23161 gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc
    23221 gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt
    23281 ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt
    23341 tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt
    23401 ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta
    23461 ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg
    23521 gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt
    23581 agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa
    23641 gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg
    23701 ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac
    23761 aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact
    23821 gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt
    23881 aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa
    23941 ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc
    24001 aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta
    24061 ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat
    24121 ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat
    24181 tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa
    24241 aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat
    24301 ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa
    24361 agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga
    24421 tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat
    24481 gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga
    24541 tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt
    24601 ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt
    24661 tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta
    24721 cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac
    24781 ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag
    24841 gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat
    24901 ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc
    24961 ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa
    25021 tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct
    25081 ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata
    25141 cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca
    25201 ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa
    25261 aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta
    25321 tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat
    25381 cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg
    25441 gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa
    25501 gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga
    25561 agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca
    25621 tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat
    25681 ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc
    25741 agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc
    25801 tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag
    25861 ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata
    25921 gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa
    25981 ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc
    26041 tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata
    26101 ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct
    26161 cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt
    26221 cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac
    26281 attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga
    26341 tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc
    26401 gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg
    26461 gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt
    26521 atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc
    26581 ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc
    26641 atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca
    26701 acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac
    26761 atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag
    26821 atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg
    26881 atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc
    26941 tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt gatattggat
    27001 tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca
    27061 aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac
    27121 tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt
    27181 agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga
    27241 agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa
    27301 cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc
    27361 agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga
    27421 aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct
    27481 tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat
    27541 tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct
    27601 aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct
    27661 ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc
    27721 aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt
    27781 tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga
    27841 tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc
    27901 aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc
    27961 ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt
    28021 cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc
    28081 cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac
    28141 gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca
    28201 aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta
    28261 cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt
    28321 aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca
    28381 cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact
    28441 attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa
    28501 tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata
    28561 aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg
    28621 aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat
    28681 cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct
    28741 agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct
    28801 aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc
    28861 agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt
    28921 ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat
    28981 tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc
    29041 tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga
    29101 ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg
    29161 tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac
    29221 aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag
    29281 gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat
    29341 aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc
    29401 aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag
    29461 cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta
    29521 gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa
    29581 ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg
    29641 accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt
    29701 cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa
    29761 aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat
    29821 gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg
    29881 cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg
    29941 aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat
    30001 atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg
    30061 gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa
    30121 caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg
    30181 ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt
    30241 atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc
    30301 gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa
    30361 tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg
    30421 ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta
    30481 acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta
    30541 tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa
    30601 acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa
    30661 aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg
    30721 gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa
    30781 aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc
    30841 atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat
    30901 tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa
    30961 tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga
    31021 tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag
    31081 aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag
    31141 gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac
    31201 gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta
    31261 aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt
    31321 atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc
    31381 gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat
    31441 ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg
    31501 gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca
    31561 gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa
    31621 gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac
    31681 cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc
    31741 tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa
    31801 gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt
    31861 ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc
    31921 gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag
    31981 gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa
    32041 atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta
    32101 gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa
    32161 aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat
    32221 gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa
    32281 aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca
    32341 actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg
    32401 gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg
    32461 gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca
    32521 tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag
    32581 ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt
    32641 gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg
    32701 ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg
    32761 gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt
    32821 agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat
    32881 cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt
    32941 atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg
    33001 agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata
    33061 cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc
    33121 ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg
    33181 cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct
    33241 gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga atccttttgc
    33301 ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg
    33361 cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt
    33421 tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg
    33481 atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact
    33541 tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat
    33601 tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg
    33661 gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga
    33721 agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg
    33781 gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg
    33841 atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc
    33901 aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt
    33961 ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct
    34021 gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg
    34081 atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt
    34141 tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc
    34201 ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg
    34261 aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt
    34321 atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg
    34381 ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga
    34441 caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg
    34501 ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca
    34561 gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt
    34621 gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc
    34681 aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc
    34741 gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac
    34801 gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt
    34861 tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc
    34921 cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga
    34981 aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga
    35041 caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg
    35101 gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta
    35161 acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag
    35221 gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa
    35281 tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg
    35341 atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca
    35401 cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt
    35461 ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg
    35521 gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc
    35581 tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg
    35641 cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact
    35701 tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg
    35761 atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat
    35821 tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata
    35881 tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct
    35941 tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat
    36001 gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt
    36061 gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc
    36121 ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt
    36181 ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga
    36241 aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca
    36301 ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt
    36361 gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga
    36421 agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc
    36481 aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg
    36541 ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc
    36601 cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa
    36661 agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca
    36721 tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga
    36781 aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag
    36841 aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg
    36901 cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta
    36961 ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag
    37021 ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag
    37081 gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg
    37141 cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca
    37201 gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc
    37261 atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc
    37321 aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag
    37381 gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg
    37441 gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat
    37501 tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa
    37561 gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa
    37621 tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca
    37681 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    37741 gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat cgtgtcacag
    37801 tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa gtcatcattt
    37861 aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc ttcgtcagag
    37921 ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc tactaaaaca
    37981 gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa agcgcgttct
    38041 aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat tgcgtaacga
    38101 cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag agtatcgtaa
    38161 gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc
    38221 aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc
    38281 caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg agttaaaaca
    38341 gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc gccttctttt
    38401 gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa ttggttcaat
    38461 gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt tagctctcct
    38521 tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca attttttgga
    38581 agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga gaatataaaa
    38641 aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa ttgctcgatt
    38701 tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc gcattttgaa
    38761 gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt tttgatcgtt
    38821 tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc atctaatcca
    38881 actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc cattaattgg
    38941 aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat caagtgcagc
    39001 ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt gcctaatcga
    39061 tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa
    39121 tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat ttcttgacaa
    39181 ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt
    39241 gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa ctggatataa
    39301 cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt
    39361 ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc gtcaagaatc
    39421 tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa aagcccatgt
    39481 tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg ttcgatgact
    39541 tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc tccgttttcg
    39601 tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag
    39661 ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac taatttagaa
    39721 gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc gaaaagcaaa
    39781 gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc accgtctatt
    39841 gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa tttttccgct
    39901 tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc ttcacgcata
    39961 cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag acctacgatc
    40021 atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa attcatcttt
    40081 gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc gtgatttacg
    40141 gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa ttgcatctct
    40201 tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta caggtttctg
    40261 tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga cattgcattt
    40321 attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa
    40381 ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt aaatcaaagg
    40441 tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag aaaactcaaa
    40501 ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca atgacaccta
    40561 aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag
    40621 ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt gtattattac
    40681 gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc ggtgctttag
    40741 atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact aaaaaaccta
    40801 aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga agtaattact
    40861 atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg
    40921 tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga tcaaccgtgt
    40981 aatggttgac ggaaaacgcg ggattgcagc taacattatc tataatgcct ttgacttgat
    41041 caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga aaaacgttca
    41101 acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag taccagttga
    41161 agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact acgctcgctt
    41221 gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa
    41281 cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg ccaaccgtgc
    41341 gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata agtcgacctc
    41401 agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa ggagaatgac
    41461 cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca tcgatgctgg
    41521 taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata aaattggtga
    41581 aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac gtggtattac
    41641 gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca tcatcgacac
    41701 accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc
    41761 tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag tttggcgtca
    41821 agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc
    41881 tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg ctcacccaat
    41941 ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg taacaatgaa
    42001 ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa ttcctgaaga
    42061 ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag ctgaaactga
    42121 tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg agttaaaaga
    42181 aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg gttcagcatt
    42241 taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc catcaccgtt
    42301 agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag aacgtcctgc
    42361 agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc cattcgtagg
    42421 tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat acgtacaaaa
    42481 cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg ccaactctcg
    42541 ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt tgaaagacac
    42601 aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat ccatggaatt
    42661 cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc aagataaaat
    42721 gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg aaacaaacgt
    42781 ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata tcatcgttga
    42841 ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag tatcttaccg
    42901 tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa
    42961 aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg ctggtttcga
    43021 atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag ctgttgaaac
    43081 tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag tagatatcaa
    43141 agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg ccttccgtgt
    43201 tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa tccttgaacc
    43261 aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta tgggtcacgt
    43321 aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt
    43381 tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc gttctgctac
    43441 tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac caaaatcagt
    43501 acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg aaaccggata
    43561 gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt gtaaaataat
    43621 tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc agacaaataa
    43681 aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat ttgaccgttc
    43741 taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa ctacattaac
    43801 agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg cttacgatca
    43861 aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag ctcacgttga
    43921 gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg cggattacgt
    43981 taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag tttctgctgc
    44041 tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc
    44101 ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat tactagaatt
    44161 agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg acgatgttcc
    44221 tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg aagaaaaaat
    44281 tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg acaacgacaa
    44341 accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta ctgttgctac
    44401 aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc
    44461 tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat tgttagacta
    44521 cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg aagacatcca
    44581 acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat tctctgcaga
    44641 agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca ctaactaccg
    44701 tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac cagaaggaac
    44761 tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc acccaatcgc
    44821 tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt
    44881 cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca tttattcgga
    44941 cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta tttattgcag
    45001 gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa agagtcagta
    45061 ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt
    45121 gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc acagacgtaa
    45181 tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac gctttgccac
    45241 gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata ggcgaaggag
    45301 ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa caccgtattt
    45361 tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg
    45421 gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg actcataaat
    45481 acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc gacattgtga
    45541 acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct ggtgtaaaca
    45601 tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg accaaaggaa
    45661 tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa ttaattccag
    45721 taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg gaaacggatg
    45781 gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca aacaaacctg
    45841 cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa
    45901 atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt ttccaagcag
    45961 gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg gttatcaaac
    46021 gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt cgtcctgggt
    46081 caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg
    46141 gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg
    46201 ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact atcaaatcag
    46261 ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg aatgtagcat
    46321 tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa atcttcggaa
    46381 ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg tcattaagac
    46441 aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc
    46501 gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa tggcgtggag
    46561 gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct aaaaaagttc
    46621 gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa
    46681 tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt cttgctaact
    46741 tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt gctgctttat
    46801 cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt gtgctagacg
    46861 ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt gaggaggtgc
    46921 ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa aatcaatgct
    46981 tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca aaactttagt
    47041 aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt
    47101 acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc
    47161 gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg ctgaataagc
    47221 actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct
    47281 acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac aacatcaaca
    47341 cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa caacggccgc
    47401 atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat cgacttcaaa
    47461 cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc aaaccgttca
    47521 gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt agcacctaaa
    47581 ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa agtcggaaat
    47641 gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga aatgaaacct
    47701 ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa
    47761 ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt agctgcatgc
    47821 cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat cggtaaagct
    47881 ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat gaaccctaat
    47941 gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc accagtttct
    48001 ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa atcagacaaa
    48061 cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc acattttgag
    48121 aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga gcatttaatg
    48181 aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc
    48241 cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga tggacgtaaa
    48301 catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga atttgcacct
    48361 acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta atttgagggg
    48421 aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa aaacagttcg
    48481 cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga
    48541 tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg aaaaagtatt
    48601 gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa acttggtagt
    48661 aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg
    48721 atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat cagaaaaata
    48781 aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta ggcatcatcc
    48841 gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta cacgaagatt
    48901 taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca acagttgaaa
    48961 tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca ggtatggtta
    49021 ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa ttaactggta
    49081 aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca aaattagtag
    49141 gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag
    49201 ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta tctggtcgtc
    49261 taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt cctcttcata
    49321 ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac ggaaaactag
    49381 gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac actgagaaag
    49441 gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa
    49501 aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc
    49561 tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg caatgactcg
    49621 ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat cttatactgc
    49681 caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat gggttgcacc
    49741 agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga
    49801 agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa aacgtgagga
    49861 aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact gccgaaatgc
    49921 ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa ttagcaacag
    49981 gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa
    50041 ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt atgactgaag
    50101 gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa
    50161 ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc atgaactatt
    50221 ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac atcgtgagaa
    50281 ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa gtagtcgaag
    50341 aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca aaatctgaaa
    50401 ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg
    50461 cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact gctaacatcg
    50521 gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc aaaaaaggtg
    50581 acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat
    50641 atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc cctcgtggaa
    50701 cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt
    50761 cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc gaaacagtta
    50821 tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa aacaaagaag
    50881 gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg
    50941 ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt gagatggaag
    51001 cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct ggccgagttg
    51061 gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag
    51121 ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc
    51181 ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt atgcaaacac
    51241 ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca aacgctaaaa
    51301 acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca atgattacaa
    51361 aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag
    51421 ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct gtttcactac
    51481 cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata
    51541 ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta gtagataaag
    51601 tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt
    51661 tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac tacgtggcta
    51721 aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc
    51781 gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt tgccgtattt
    51841 gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa
    51901 aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc caattgcaga
    51961 ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag aagtacctgc
    52021 atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt
    52081 tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa
    52141 cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt
    52201 caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca tttcgacttc
    52261 tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc
    52321 ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa
    52381 ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac aacgttacag
    52441 ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa atgaatatcg
    52501 aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa acaattcatg
    52561 gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag
    52621 cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa cttgtattga
    52681 acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact gttgaagtac
    52741 cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc gaattagctg
    52801 caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt cgttatgttg
    52861 gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat
    52921 agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa aatacatttt
    52981 tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata gtagtacaat
    53041 aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac acgtcaaaag
    53101 agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg cttgaacgtt
    53161 tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg tgtaacgcta
    53221 gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga agcagcacaa
    53281 gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt
    53341 gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa
    53401 aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc gatccaaaac
    53461 atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg
    53521 gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga cacgtaggat
    53581 ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt gaagatgcga
    53641 agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa gtaatcggtg
    53701 cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg gttgctgctg
    53761 gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact tctaaatctt
    53821 taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg aaacaattga
    53881 aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta atcggataag
    53941 gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta tcggacgtcc
    54001 tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca gttcagtggt
    54061 taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc tagtggacgt
    54121 tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc aaacctatga
    54181 aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg
    54241 gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt
    54301 caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc cgtcgtttac
    54361 caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat cttgatgtat
    54421 tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg
    54481 tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac aagaaattaa
    54541 ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg gctggtggat
    54601 caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa aagacattag
    54661 atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg cacatattac
    54721 tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat ttttcaacat
    54781 gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa tgggggtttc
    54841 accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg tgcctcgatt
    54901 tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata
    54961 tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat tcaatcaata
    55021 cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga tcggtttgat
    55081 tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag aaaaaggtat
    55141 cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc ctgaaagtat
    55201 cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat ggaaatctgc
    55261 tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg taacattttt
    55321 ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac
    55381 aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta tctttgctag
    55441 ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat tcggttctga
    55501 aaactggtac aaagtgatga tggagatctt taactataat acagcacctg gtgctacgat
    55561 ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag taaaccctga
    55621 gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac gccctggtaa
    55681 ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg gtgcagtctt
    55741 ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc ttccacaatc
    55801 aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag aaactgcgaa
    55861 acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt
    55921 gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa acaatgaacc
    55981 tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa atcattgatg
    56041 cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg caaaacgaaa
    56101 cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt cctgatgaag
    56161 taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa ggcttcttat
    56221 tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg ctaaaagatt
    56281 tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac
    56341 gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa attttcaacc
    56401 ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat caaagagaag
    56461 atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa
    56521 tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct cgtgaaatcg
    56581 atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc ccgtagccga
    56641 cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata tgtcagtccg
    56701 acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga actgcagttt
    56761 ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa
    56821 gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa
    56881 gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat tcgtatgcat
    56941 tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata cgatcttaac
    57001 cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca ggaggtatta
    57061 tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta
    57121 aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt caaggataat
    57181 tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg ataaacgtgt
    57241 agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa agatcttagc
    57301 tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc
    57361 tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt
    57421 gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc gccgtggatt
    57481 gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc
    57541 tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca tggcagcaaa
    57601 aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg cacatatcca
    57661 ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg cattagcgtg
    57721 gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca
    57781 aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga ctgttgaagt
    57841 aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac aagctacagg
    57901 tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg cccgccctcc
    57961 aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta acgtcattga
    58021 acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc gaaaaaccaa
    58081 gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa
    58141 gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct tctttgccag
    58201 gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct accatcaaag
    58261 gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg ctgaaaatgt
    58321 atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca gtaactgctg
    58381 gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac atctgtagtg
    58441 tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt ggttatgttc
    58501 aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc gattcaatct
    58561 acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga caccgtgaag
    58621 attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg ttggaagcaa
    58681 tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac ttaactgatg
    58741 aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg
    58801 aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg aaacgtgcag
    58861 gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc aaagtccgta
    58921 acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta ggtttaggtt
    58981 tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt tatcgtaaac
    59041 taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact gatttattaa
    59101 tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca actgcagaaa
    59161 aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg
    59221 tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt
    59281 tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc ggatacacac
    59341 gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc ttagaacttg
    59401 tctaaaatca cactaataac atcactttat agatgattct tttagagtgt tatgatgttg
    59461 gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg cttggggagt
    59521 aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac agatatgttt
    59581 gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt tttttgatag
    59641 tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat tgaattagaa
    59701 aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga tgtctccttt
    59761 acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg aaaatcgaca
    59821 cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa agtgggaaat
    59881 caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat ggtttttcaa
    59941 aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt tggtcttgaa
    60001 aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt
    60061 agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca aaaacaacgt
    60121 gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga tgaagcaacc
    60181 agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa
    60241 gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc taatgccaat
    60301 cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa agaaattttt
    60361 tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga aaaactgaag
    60421 agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga agaaaggatg
    60481 gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag ccgaatacac
    60541 catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac agttatactg
    60601 cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg aatgcgcttg
    60661 ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg gatacggata
    60721 ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac
    60781 agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca
    60841 gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg ctggatgaaa
    60901 gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga gtagcaatcg
    60961 ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg gcaggacttg
    61021 atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag gaacagcaga
    61081 tcactattgt attagttacg catttaatgg atgatgtagc caattttgca gattatgtct
    61141 atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc tttcaaaaca
    61201 ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt gcagaagaat
    61261 taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa ttagcagacg
    61321 ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt tggtcgttat
    61381 atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt agccagtttt
    61441 tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt gttggcattt
    61501 ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat ccgaggcatt
    61561 cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt tacaagtggc
    61621 ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt gatgaacggc
    61681 ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact cacgcttact
    61741 acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta
    61801 cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca
    61861 ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt
    61921 gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc tttatttgtc
    61981 agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg ctacaaaggt
    62041 ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac gatggttatc
    62101 attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac gttcagtggg
    62161 ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc agtgtatgac
    62221 aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt atctgaaaag
    62281 aaagaacgaa aaaattttat agaaaaatca ttattccaga taacctttta ggaacaaagg
    62341 agagaaaaac atggtacgtt acaaagcaat cattgcgtac gacggaacaa attttaatgg
    62401 attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa cattacaaaa
    62461 aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg ccggagtaca
    62521 tgcgatcggg caagtaatcc attttgatta tccagaggag cgtccgttag aacgaatgcg
    62581 tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag aaattgtttc
    62641 agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc gagtggatat
    62701 agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt acccacttga
    62761 tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt tcacctcttt
    62821 ttgtgcatca ggaagctcga tagaggataa aatacgtacg atctacgagg ctaagatgga
    62881 ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct tcttgtataa
    62941 gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat tgcctgaaga
    63001 tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta cagcacaccc
    63061 tgaaggactt tatttgtatg aagtgaaata tgaataagag gttatgatag aagtggtcag
    63121 cttcaagaaa taagaaggta tttacgaaaa ttacttctca aaattttgca aaatttcggc
    63181 ttattttcga agaagctact tctggaacac cgtttaagga agaggtcgtg aaatcagcgt
    63241 tttgacctga gtattagaga aacaaacggg aaaatagctc ctcatatttt gccgtttgtt
    63301 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    63361 atttaactct gagtcacaaa gagcaatttc aaaaaacagc ttctcatctt tgatgaatat
    63421 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    63481 aaccaaaaaa cgatggaacg ttgttttgat tgcccttggt gcggacagtc tgaacaggtt
    63541 ccatcgtttc tttattttct acgtcttctt ctgtgtgtgt ttcgtggttg ttcttctatt
    63601 gctggatcaa tcgtgccata aaaggggcga ttgcggtcaa ggtaccagac ataaagaata
    63661 aacaatgcaa tacataggac aaaaaggatt gcacctatcg taaatccttc aggcaagtaa
    63721 ctgaaagtga cagtatgttt acctgctgga acttttatca tcaagaatgc gtctttgaaa
    63781 ttgctgacct tgactggttt cccatcaatt ttagctttcc atcctttgtc ataaggaata
    63841 gttgtcacca acatggaatc ttcagcggta tttacagtcc cagaagcttt tctgccagtg
    63901 gcttccattt cgacaccttt ttctttgatc tgattcattg cggcttgata ggctacaaca
    63961 tctagaccaa cgactttagg ctccatgaaa cttatttctt tcgtcccata aaaacttgca
    64021 gtaaatgtca ctgttgtctc ttgaggataa tagccaagat catagtattg accagaaatg
    64081 ttgatttgcg tttttcgcga tgtaccattg acagtgatcg tcgcactaga actttctaat
    64141 tgcgcaaaat tggttgggaa caaacttaag taagcttgtg tatttgcagg cacatggact
    64201 tcccaagtga gctcttttgg ctggttatct gctgtttctg agtaagtgac gccagtcgtt
    64261 gtctttgtga tcgtcgtatt caactgattc ttcaaagtag gtgtatagaa tgtaaaatac
    64321 tgttcattta gaccagacaa tgtattgatc aagtttgtct gactggtcaa attatctgtc
    64381 actggctgct caacatcatt aacggacaac ggtgcagtaa atgctagtgg caatgcattg
    64441 acattctgat acagtgtata gtcgccgctt tgaccagctt ggcggaagcc atatttactg
    64501 aacgtgcttt cgtttttaga aatgttatat ttgattcctg taaacccatc catcagcaaa
    64561 gtattattgt tataacggat attcaaattt gtccctcttg aacggaaacc taatttatcc
    64621 aggtaagagg aagaattacg atttcttata gaagaaaaca aactgatacc actgtaacca
    64681 tagttgatcg aatcgtttgc agatactgga tccagatttt ccaagcggta aaacgagtcg
    64741 gaatcttttt ttgtttcatt tacaagagct ttcaagtcag gataaggatc agtatataaa
    64801 ctgcgggaag cataattcca atcatcaaga atgccatgga tcatactgtt cgtattgacc
    64861 agtgcttcag cagacatgat caataataac aatatactca gatgattgaa tgaaagtttt
    64921 ttatgctgat aaaatcccac agcaaaagca taaatcccta agaaaaccac tgtcaataca
    64981 aaggaagtca tcgtcacata ggtgtagctt ccttcaggtt tgatgcccca tgccaccgca
    65041 aagagagcag ctacgaccaa aatgattcct aacaacagcc cacgatcgtc ttgttctatc
    65101 ttttcccatc cttttcctgc aagcaggagc actaagaaag agaacagata ggcataacgg
    65161 aataaaaaca tattcggtgc atgcatacca tgccagaaca aattcaatgg aacaagataa
    65221 aaacttgcga taagtatggc gaaaagactg ccgtacaaaa atttattttt caacggtact
    65281 ttccgactgg caaaataaaa cagacagagg actaatggta gcagacctgc gtaaacgaac
    65341 ggaatcgatc cgtattttgt cgtgtcataa acaccaatca tatttttcat gattagatcc
    65401 agataagaag tcgcttctgt tttcaacgta gtgatctgac tcagtgcctc gccatttgac
    65461 cgaagatcca gcactgctgg caatacaatg atcattgaag cgccgcctgc cagtaaagaa
    65521 gtgataccat aaggaacaat cgttttttta tattctgacc agtttgtcaa tagacgggcg
    65581 ataaaatata agaaagaaaa tacgccaatc ataaaaccca tataaaaact ggataaaaac
    65641 aagagtaaat aactgataaa taaaagtttc ggccgtttaa gatccataac acgatgaatc
    65701 cccaaaatca ctaatggaag ataaaaaaat gcatccagcc acatgatgat ctccgaatgt
    65761 gcagtgataa atgagatcaa tgcgtaactg acgctaagtg caacatgccc ccagcgagga
    65821 atcttgaacg tacgtgcaag aaaccaaaaa gccaatccgg cacagccgac cttaagcaaa
    65881 gtcagaacat acagtgcatc tggcatcatc tggtttggaa aaaagaacac cagtggcgtg
    65941 aagatcccgc ctagatagta agaaattaaa gataaataat tcaatcctag agaagcattc
    66001 catgtataaa aaatactttg ctttccaaga agaacatttc ggaagctggc atgaaaatta
    66061 gaaaactgtg aaaaggcatc acttgccaaa atacttctgc tgcttccagg atagattccg
    66121 atacttagat aaacaagtgc taaaatcaaa aacggaatca ggaaactagc tgctgcgtag
    66181 cgtccgtttt ctctaaagaa tgactttaaa ttttttttca tatttatttt ccaccaaatt
    66241 tctatgaagt tgtattcagt ttatcatatc cttgtttttc tctgcttaaa aagagtctag
    66301 ttttcacaaa aaaataaaat ttagaataaa taacttcata atttgttcgt ataacaaaaa
    66361 taaacgcaaa aaaagggtag gaatcaaaag tcccaccctt aaaaattttt attaaataaa
    66421 aaaccgttca ctatctgaac ggtccaagta tcattcaatt aaacgcgttc aacttttcct
    66481 gatttcaaag cacgagttga cacccaaact tttttaggtt ttccgtcgac cataacgcga
    66541 actttttgta agttaggttt aacagtacgt tttgttgagt tcatagcgtg tgaacggttg
    66601 tttccacttt ttgtcttgcg acctgtaaag taacaaactt ttgccatggt aatttcctcc
    66661 tttgccttga tttggagcaa tagaataatt cagctcatac cagactaaca tatcatatag
    66721 aatgggaatt tgcaagtaca ttttcataga tacaattgac aattgagaag aataattgta
    66781 aattaaacaa ggttatacct gaaattttgc aaggtttttg agtactttgg gtaactgcct
    66841 tttatttcaa gttgcgctat gataaaatat gatagaagaa aaatgggagt tttttacccg
    66901 ttaaggaggc tcattcatgg ctgtaaaaat tcaaacgtca gctggaacca ttgaaattac
    66961 caatgatgta attgcaacag ttgttggtgg cgccgcgaca gatgtgttcg gtgttgtagg
    67021 aatggccagc aaaaatcaaa ttaaagataa tattactgag attttgcgta aagaaaatta
    67081 ctcaagagga gtagttgtcc gccaagaaga taatggtatt gctgtagatg tttatacgat
    67141 tatcagctat ggtacgaaaa tctctgaagt ttcccgcaac gtccaagaaa aagtgaaata
    67201 caatctagag gcattattag gcgtaactgc caattcagtt aatgtctttg tacaaggtgt
    67261 tcgtgtactg ccagactagg caggagactg gctggtaact cagcagtcga tctgaaggag
    67321 gattatctag gtgagtgtaa cagaaataag tgcaagccag ttccaggaaa tggtccaggc
    67381 aggtgcgaat cgtctgcaag taaatgcaga atacgtcaat tcattaaatg tattccctgt
    67441 gccagatggc gatacgggaa caaatatgaa tctatctatg actagtggag ctaaggcagt
    67501 agtagattca acctcagaaa aagtaggaga attagcagca atgctttcaa aaggattgct
    67561 gatgggtgcg cgcggaaatt ccggcgttat tttgtctcaa ttgtttcgtg gattttctaa
    67621 gcaaattcca gatgttgtct cattaaatgc ttctgatctt gctgcagcct tcactcatgg
    67681 agttgaaact gcgtataaag cagtaatgaa accagtagaa ggaacgattt tgacagtagc
    67741 tagagaagca gcgaaagctg gagaaaagaa agcaaaaagt actgatgatg tcgttgaagt
    67801 aatgacagct gttgtaaaag gagcaaagcg tgctttagct aaaactccag acttgcttcc
    67861 agtcctaaaa gaagtcggtg tcgtagacag tggagggcaa ggactgctgt ttgtttatga
    67921 gggcttttta agtgcattga atggagaatt ccaagcggat gaaacatatg aaccttctcc
    67981 agcagaaatg gatgatatgg tcaatgcaga acatcatcgc agtgtccaag gtcaaatggc
    68041 gacagaagat atccattttg gttactgtac agaaatcatg gtaaaaatcg gagaaggacc
    68101 tacagtcgat agtacattcg attacgaaga attccgtaac tatttagatg gtataggaga
    68161 ttctttactt gtggtcaatg atgacgagat catcaaagtc cacgtgcata cagaaaaccc
    68221 aggagaagtc ttgaattatg gacaaaaatt cggttcatta gtcaaaatca aagtcgacaa
    68281 catgcgcttg cagcatgaga cgattttgga acacgatgaa gaagtgaacg agtttgcgaa
    68341 aaaagaaata ccacgtatcc cgtatggtat cgtagcgatc gctgctggta aaggcgtcca
    68401 agagctgttt gaaagtttag gcgctaatta tgttatcagt ggtggacaaa caatgaatcc
    68461 aagtacggaa gacattgtta aagcgattca agaagtaaac gcagataaag tgattgtctt
    68521 gccaaacaat aagaacatct tcatggcagc tgatcaagca gcagaagtag cagatatccc
    68581 agtagcagtt gttccttcaa gaacgatttc ccaaggattg acagctatgc tttcattcaa
    68641 tgatcaagct tctttagaag aaaacaaaga aacgatgaca gaaatgttgt ctagcgttgt
    68701 gagcggtcaa gtcactcacg cgatcagaga tacctcaatt gacggtgtag agatcaaaga
    68761 aggagacttt ttgggcatga tcgatggaaa aatcctgatc tctaacccag atatcacagc
    68821 aacagcgtta agtacactag aacaaatgat caatgaggac acagagatcg taacgatctt
    68881 ggcaggtgat gaaggaacag ttgaacaagc aaatgaattt gctgagaaac taacagaaaa
    68941 acatcctgat ttggaaatcg agatccacca aggagatcag cctgtctatc cttatctatt
    69001 ttctgctgaa taatttctgt gtcagaatat ctttctaaca cagtattcat tcataaaagg
    69061 ccttcggtta gtgatcaggt atgatcgctg accaagtcct ttttttgtta aaggagggag
    69121 gaaaaattta tgcgttcttt agatgatcca atcactatgc tggcaggagt cggagaaaaa
    69181 agagcagcta gtcttgccag cttgggcatc caaacgattg aagacttact gacttattat
    69241 ccttttcgct acgaagatat tcaggaaaga aatctaaacg agatccagga tcaagaaaag
    69301 gttactttga aaggaatcgt agtctcgcct cctgtgatga atcgatttgg ttataaaaaa
    69361 agccgattgc aatttcgtat gatgcaagaa catgatgtgt tcaacgtttc tttttttaat
    69421 caaccttatc taaaagaaaa agtcatttta tcggaggaaa tcgccgtata cggtaaatgg
    69481 gatgcaaagc gtaaagcctt gaacgggatg aagattttag gttctcaaac aatggatgac
    69541 ttttcgccga tatatcacgt caacaaatca attcgtcaaa cgacattagt tgatctaatc
    69601 aaaaaagggt ttcaagaata cggcacattg attgaagaaa atctgccaaa caaccttgtg
    69661 gagaaatatc gactgcttga ccgagcttct gctgtaagaa gtatgcattt tccaaaagac
    69721 cacgaagaaa accatcaagc caaacgacgt gtcgtttttg aagagttctt tttatttcaa
    69781 atgcgtcttc aaggattgaa aaaagcagaa aaagcagaaa cgaatggttt ggagatcctt
    69841 tatgatgtcc aaaaattgaa acaatttact caaaagctac cttttgaact gacaaatgca
    69901 caaaaacgtg taacgaatga aatctgtcgg gatcttcgca gtccgcagca tatgcagcga
    69961 cttttgcagg gagatgtagg tagcggaaaa acagtggttg ccgcgattgc gttatacgct
    70021 accgttacag caggtttcca aggtgcgttg atggtgccta cagaaatctt ggcacaacaa
    70081 catatggaaa gcttgcaaca gctgtttgat cccaatgaag taaccatcgc attgattacg
    70141 gggtcaacga aagcaaaaga acgacgtgag ctccttgaaa aactagagca aggggaaatt
    70201 aacgtcgtga tcggcacaca tgcattgatc caagatggag tcgaatttca gaatttaggt
    70261 ctagttatta cggatgagca gcaccgtttt ggcgtgaacc agcggaaagt acttcgtgaa
    70321 aaaggctggc gaccagatgt cttatttatg acagcgactc cgattcctag aacattagct
    70381 attacagcat atggtgagat ggatgtatct atcattgatg aacttccagc tggacgtatc
    70441 ccgatcgaaa cacgctgggt aagaacacct caattggaca gtgtactgga ttggacgaaa
    70501 aaagaactgg caagaggaca tcaaatgtat gtcatctgtc cgctgattga agaatcagaa
    70561 gcattagatg taaaaaatgc agtggaaatc tatgaaaaac ttcgtgaact atttgcacca
    70621 aaatatgaag ttggcttgtt acatggtaaa atgaagaatc aggaaaaaga agtaatcatg
    70681 gaagccttca aagacaacca gatgcagata ttagtttcga ctactgtaat tgaagtaggg
    70741 gtaaacgtgc cgaacgcgac cgtcatgctg atcatggatg cggatcgatt tggattggca
    70801 cagctgcacc aacttcgtgg acgtgttgga cgcggaagtg atgcttccta ctgtatctta
    70861 gttgctaatc ctaaaaatga actaggtgtg gaacggatga aaatcatgac agagaccacc
    70921 aatggttttg tactaagcga aaaagatctg gaattacgtg gtcctggtga agtatttggc
    70981 ttccgtcaat ctggtctgcc tcaatttgcg attgctgact tggtgacgga tggaaatgtt
    71041 ttggaggtag ccagagaaga ggctgcagcc ctttggcaaa taaaggattg gcagatgctt
    71101 cctgagtatc agcctttagc gcaaagcctc caaatagaaa atcaggataa tcgttatttc
    71161 gattagcaga ctgtgctata ctttatactg atgacggaaa aaggagtgga aacaatgaaa
    71221 attgccgtag atgcaatggg cggagatcac gcacctcaag caattgtaga aggcgtggct
    71281 ttagcgcagc aagattttcc agaagtagaa ttcttacttt acggaaaaga agcagaaatc
    71341 aagaaatact taacaaatga aaaaaacatc acgattatcc atacagacga aaaaatcaat
    71401 agtgatgatg agccagtaaa agcaattcga cgaaaaaaga ctgcatcaat ggtccttgct
    71461 gcacaagcag tgaaaaacgg tgaagcagat gctgtatttt cagcagggaa tacaggcgcg
    71521 ctactagcag cagggttgtt catcgtagga cgtatcaaaa atatcgaacg ccctggcttg
    71581 atgtctacct tgccagtagt agggaaagaa ggtgccggtt tcgatatgct ggatcttggc
    71641 gctaacgcag aaaacaaacc agaacatttg ttgcaatacg gtattttagg ttctttttat
    71701 gcacgtaaag tccgaggtat cggacgtcct cgtgtcgcac tcttaaacaa cggaacagaa
    71761 gcaacaaaag gtagtgaagt gacgaaaaaa gcctatgaac tccttcagaa tgaaacaagc
    71821 ttgaatttta ttggaaacgt agaagctcgt gagctactca atggtgtggc tgatgtagtg
    71881 gtcacagatg gatttacagg caacgctgtt ttgaaatcta ttgaaggaac agcaatgaat
    71941 atgatgaatc tattaaaatc tgctattcta aatgaaggga tcaaaggaaa aatgggcgct
    72001 atgcttttaa aagacggctt acgttctttg aaagcagaaa tggattattc caaacatggc
    72061 ggtgccgttt tgtttggctt gaaagcacca gtcattaaaa ctcatggagc aacaggcccc
    72121 gatgctgttc gctatacgat ccgccaaatc cacacaatgc ttgagacaga tgtagttgga
    72181 caattagtcg aacaatttga aaaaagcgaa gaatagtatt tggatatctg aagcgatccg
    72241 ttccattgaa aaaaaggtgg acattcctga aaaatgccag taaaattagc catggtgtag
    72301 gtaattgaca agtgtatgat tcttggaggt gaaaaagttg actcgcgaag aagtatttaa
    72361 caaagtagcg aaaattattt caaatcattt tgagatagat accgataaag tgacggatga
    72421 attgaacatc aaagatgatt taaaagctga ttcaattagt attatggaat tcgttttaga
    72481 gcttgaagat gaattcggta cggaaatttc agatgaagac gctgaacaaa ttgaaacagt
    72541 cggtggcgca gtagattata tcagcagcca tcttaaataa tttcaaataa aagcaaagaa
    72601 ggataaaaag ctgcatgcag ctttttatcc ttcttttttt aatctttttg acacgtaaaa
    72661 gtcatttttc tgataattct ggcgataaac agaaattatg ttgcaaagta aagcggtttt
    72721 ctatataatt attaataatt cattacacaa agataagaat aacctttgtt ataaaacgat
    72781 ttattctgtg taatgtcctt ttttattaag aaaaacttcc atttaggaag cagagagggg
    72841 tattcgattg tccgataacc aattattaga agttcagaac ctacatacag gattccgact
    72901 caaagatgac tattacgatg cagtagatga cgtttcattt actttagaca aaaacgagat
    72961 tttagcaatc gttggggaat ctgggtgcgg gaaaagtacc ttggctacta cgattatggg
    73021 attacttgat cctaataaca caaaaattac aggtgagatt ttatataacg atctgaattt
    73081 aatcgacctg aacgaaacgc tctataataa aatccgcgga aatgatatcg gaatgatttt
    73141 ccaagatccg ttgtctgcat taaatcctct catgagaatc gaagaccaga tcaaagaagg
    73201 actttcctat cacacaaaga tgaatgcaga acagcgtcaa gctcgtgcac tagaattgct
    73261 agaacaagta gggattccta atcctgcgag agttggacgc caatatcccc atgaactatc
    73321 aggcggtatg agacaacgtg tcatcatcgc aatcgcaatc gcctgcaaac ctccaatcgt
    73381 cattgcagat gaaccgacga cagccctaga tgtaaccatc caagcacaga ttcttgattt
    73441 actgaaagac cttcaggaag aaacaaaaac agggatcatc ttgatcactc atgacttagg
    73501 agtcgtagca gaaatggctg atcgagtggc agtgatgtat ggcggacagt tcgttgaagt
    73561 agcaggtgta aaagaactgt ttgatcatcc taagcatcct tatacacgct ctttactgaa
    73621 ctctatacca caagaaggaa atcatgatgc agaactccat gtgatcgaag gtgtcgttcc
    73681 ttctctgaaa aacatgcctc gtaaaggttg tcggtttgca ccacgtattc catggatacc
    73741 agcaagcgat catgaagaag agcctatcct tcatgaagtc gcaccgaacc acttggtacg
    73801 ttgctcctgc tataagcatt tccactttag agacgaaaaa ggagaggtgt agatgacaga
    73861 attaatcaca atcaaagacc tgaaagttca ttaccctatt cgcagtggat tcttcaatcg
    73921 agtaacggat catgtatatg ctgtagatgg tgtggatttt attatcgaaa aaggaaagac
    73981 atatggttta gtgggagagt ctggctctgg aaaatctact acgggaaaag ccgttgtggg
    74041 attagaaaag gtaacagccg gacaaatcat gtatgaagga aaagatgtaa cgaagcgaag
    74101 caaccgcaaa aaaatgggtt ataacaagga tgtgcagatg atcttccaag attcaatgtc
    74161 cagtttgaat cctaaaaagc gagtgttgga tatcattgct gaacccatcc gaaattttga
    74221 acggttaagc gatcaagaag aaaagaaaaa ggtaaaagga ctgctagata tcgttgggat
    74281 gccggaagat gcgctatata aatatcctca tgaattttca ggcggacaac gtcaacgtct
    74341 aggagtagcc cgtgctgttg ctaccaatcc aaaactgatc gtagccgatg aacctgtatc
    74401 tgctctggat ctatctgttc aagcgcaagt attgaatttc atgaaacgaa tccagcaaga
    74461 atacggcctc agctatttat tcatttcaca tgatttaggt gtagtaaaac acatgtgcga
    74521 caatatcgca attatgtaca aaggtcgatt tgtagaaatc ggtacaagag aagacattta
    74581 taatgacccg cgacatatct atacgaaacg tctgctttca gctattccgc aaatcgatgt
    74641 agaaaatagg gaaagccaca aagaaaatcg acgaagagta gaacaagagt atatccaaaa
    74701 ccaaaaagaa tactatgatg cgactggtcg agtctacgat ttgcgtacga tcacaactac
    74761 acacaaagtg gcgttgaaag atggaggtgc aagctaatgt ggaaaacgat tctgcgacgt
    74821 gttttattga tgatcccgca agttattatt ttaagtgttc tgatcttctt attagcccaa
    74881 gcgatgccgg gggatccatt taccggtttg atcaatccaa atcaggatcc ggcagtgatc
    74941 gaacagatgc gacaagcagc tggcttgaat gacccatggt atgtccagta cttccgttgg
    75001 atcggaaatg ctttgcaagg cgattttggt caaagtttct tatacaaacg ctctgttgct
    75061 gacttgatcg gcgaacggat catcaacact ttatatctat cgatcttgac agttgtcctt
    75121 acttatttga ttgctgtgcc tctggggatg cttgctggcc ggtatcaaaa ttcaatcttg
    75181 gataagattg tcgtaatcta caacttcttt agttttgctg ttcctttatt tatcttcggc
    75241 ttgatcatgc tatttgtttt tggttatcgt ttaggctggt tcccaacaag tggatcacaa
    75301 acactaggtt tgcaaggtgg atttatcact caatggatag accgtttgaa atatattctg
    75361 ttaccatcaa ttacccaagc atttttggcg actgccgtca caatccaata tctgcgaagt
    75421 gaagtaatcg attcgaaatc gctcgatttt gtcagaacag cccgttccaa aggggtaccg
    75481 acgaataaag tattcagtcg acatattttc cgaaatgcag cgttgccgat ggcttctcaa
    75541 atggggtatg aaatcacttc gttgattgct ggttctgtag ttatcgaaaa gatttttggt
    75601 tatccgggag tcggaaaact atttattgat tccatcggcc aacgggacta taccgtgatc
    75661 acatccttag tattgatttt aggaatcgcg acgctagtgg ggacattact ttcagatatc
    75721 atcatgagta tcgttgaccc gcgtattcgg attcaataaa accaatcaga aaggagaatg
    75781 gatcgatgag tgaaaaaaac aatacagcag ttcaagaaag tattcctcct atggggatac
    75841 gaatgattgc tcgagaattt aaaaaagata aaattgctat tttttcatta ggtttgctag
    75901 tggttttatt attagtgatc tttatcggtg cgatgctgac cgatcaagac aaagtgatgt
    75961 atgttagtat cttcgacaaa tacgctgcac caggaacagt ttcgacacaa ggacaaaagt
    76021 tcattctggg agcagatgaa ggcggacgtg atgtattagg acaattgatc ataggtgcta
    76081 gaaactctgt tacgatcgga tttgcgatca cgatccttac ttcgatcatc ggggttggat
    76141 tagggatcat ctctggctac tacggaggga tcattgacaa catcttgatg cggatcgttg
    76201 attttatcat gattctaccg atcatgctga tcattatcgt cttcgtatcg attatttcca
    76261 gatacaatgt ctggtccttt atattcatca tgagtgcatt ttattgggta gctaaagccc
    76321 gattattccg aagcaagaca ttatctgaag caagaagaga ttatgtcagt gcctcaaaaa
    76381 caatgggaac caatgatttt aaaatcatgt tccgcgaaat catgccgaat ctaagttcat
    76441 taatcattac taatttgacg atgaactttg cggcaaacat cggtatcgag acaactttga
    76501 ctttcttagg tttcggcttg ccggcaaatg tacctagttt aggtacattg atcggttatg
    76561 caagtaacgg agaagtctta gtgaatcggc aatggatctg gttacccgca tcaatcctta
    76621 ttttagtttt gatgttgagt atcaattatg ttggacaagc attcaagcgt tctgcagatg
    76681 cgcgccaacg gttaggctag tatagaagcg aagggggaaa taggaatgaa gaaaacgatt
    76741 tttggatttg cagcgctgat gtctgctgtc gttcttgcag catgcggggg cggaggtaca
    76801 gatacctctg gaaacaactc tggaggcaac ggcaataaca aagaggaatt aacttttcca
    76861 attgcaacaa aaaataatga tgatgcaatc gaagacggcg aattagaagc tgcggtcgca
    76921 acggacagtc agtttaaagg attgttccag tgggagtttt accaagattc atatgatgca
    76981 gcttttatgg ctccttctca tgaaccgcta tttgcaaacg atgacgattt cagaatcaca
    77041 gacgacggag ctgcaacgct tgatctagat gaagatacga aaaaagcgac aatcaaagta
    77101 aaagacaacg tcaaatggtc agatggtgaa gatgtaacgg cggacgatgt gattttccct
    77161 tacgaaatca ttgggaataa agattacact ggtatccgtt atgacgacac tttccgtaac
    77221 atcgtaggta tggaagaata tcatgatgga agtgccgaca caatctccgg aatcaaaaaa
    77281 atagacgaca aaacagttga aatcgaatat aaagaagtaa acccaagcat gcttcaatcg
    77341 ggtggtggta tctggtcata tgcagcacca aaacatacgt tagaaggtgt agcaatcaaa
    77401 gatatggaat caagcgatca agtacgtaaa aatccagtga cttttggccc ttattacatg
    77461 agtaatatcg ttgcaggaga atcagtagaa tacttgccaa acgagcacta ctggaatggt
    77521 acgcctaaat tgaaaaagat cacgatgaaa tctgtaccga tggcaagtgc aactgaagca
    77581 ttgaactcta aattatatga tatgattttc caaatgccaa cagatactta cgatacctat
    77641 aaagaaattg ctggctatac aaacttaggc cgtgaacaaa cttcttatac ttatctagga
    77701 ttcaagttag gtaaatggaa tgcagataca aagagtgtgg aatacaatcc agattcaaaa
    77761 atggctgata aatcattacg tcaagcaatg ggctatgcat tagataataa tgcagtaggt
    77821 gaacgtttct atgcaggttt gagaagcaat gcgacttcat tgattcctcc agtattccag
    77881 tcataccatg atgatgagat gaaaggattc acacaagatc tagacaaagc gaaaaaacta
    77941 ttagatgatg ctggctacaa agatgtggat gacgatggtt taagagaaga caaagatgga
    78001 aataaactaa caatcaactt tgcttcgatg tccggtgggg aaacagcgca accattggca
    78061 gattactata tccaacaatg gaaagaaatt ggtttggatg tccaatacac aacaggtcgt
    78121 ctgatcgaat tcaattcttt ctatgataaa ttagaaaacg atgatcctga aattgatgtt
    78181 taccaagctg cttggaatac aggaactgat ccaaatccaa gtggactatg gggaccaaat
    78241 gctgcgttca actacactcg ttatgaatca gaagaaaata caaaattgat caacgatatc
    78301 agttcaagca aagcatttga tgaagagtac caaaaagaag catatcaaaa atggcaacaa
    78361 tatgcatttg aagaagcatt tgcgattcca acgttgttcc gtaatgaagt attgccagtc
    78421 aacgaccgtg tcaaagactg ggattggtct tatgatgtac caaacccatg ggcaaatgtt
    78481 tctgttacag cagattcacg ttcataatca ctaaaaatga ccgaaaaagg gcgatgtcct
    78541 ttttcggttt tttgatattt ttctgtttat ttctctaata ttcaggtcaa ggcgctgatt
    78601 tcatgacttc tccaataaac ggtgttcaaa gtccagtttc tcggtattaa gtcaaattta
    78661 tgcaaaaata tggaaagcca ttttcacaaa attttttctt catactcgaa actaaacgaa
    78721 tttttcacaa cttctcgtca atcactagga ttattaaagc tttacttacc ttttgaccga
    78781 accatttcaa atttgacaaa aaaccagtat aataaggaag tatcgaaaaa atatccataa
    78841 aaggaatgtg gaaaatggac aaccagctga caaaagaact aaaagaaaac tataacatcg
    78901 tttttcacga tctaaatttg cttgaacaag cttttaccca ttcatcctat gtgaatgagc
    78961 atcgcaatct gcaattatcg gataatgagc gacttgaatt tttaggagat gccgttttag
    79021 aactgatggt ttctcagtat ttattccgtt tatatcctca tttgccagaa ggaaaattga
    79081 caaaaacacg agctgcgatc gttcgcgaag acagcttgtc gaaatttgcc aaagaatgtc
    79141 actttgatca atatgttttg ttgggcaagg gagaagagaa ttcaggagga cgtacgcgac
    79201 cagcattgct ttgcgaccta ttcgaagctt ttttaggcgc gttatatctt gaccagggat
    79261 tcgacacgac ccatgcattc atcgaaaaag tgatttttcc taaagtcaaa gcaggtgctt
    79321 tttcacatga gatggatcat aaaacaaaac tacaagaagt cttgcaaaaa tcaggtgatg
    79381 tatcaatcga ataccgattg atcaatgaag aaggtccagc tcatgaacga gtattttgga
    79441 tcgaagttta tgtagatgat caattgattg gcactggaca agggaaatcg aaaaaattag
    79501 cagaacaagc tgcggcggaa aatgcattgg cagcactgta aaagtaagtg agggaaagct
    79561 ttgtatttga aacgaattga aatagccggt tttaaatcat ttgcagacaa gacagtcatt
    79621 gattttgaaa atagcgtcac agcagtggtt ggaccaaatg gcagcggaaa aagcaatatt
    79681 acagaggcta tccgctgggt attaggagaa caatctgcta aaagtctgcg aggcggaaaa
    79741 atgccggata tcatctttgc cggttccgat acccgtaagc agctgaatat tgcagaagtc
    79801 acagtgattt tagacaatag tgatcattat ctgccattgg agtacaatga gatcagtgta
    79861 acccgccgtt atcgacggac tggggaaagc gagtttttca tcaataaaca aagctgcaga
    79921 ttaaaagata tccaagaatt gttcttagat tctggattag gaaaagaatc gttctcaatc
    79981 atttctcaag gaaaagtaga agcgattttc agtagtaaac cggaagaccg tcgtgggatc
    80041 tttgaagaag cagctggtgt cttgaaatac aagcagcgga aaaagaaagc agaacagaag
    80101 ctttttgaaa cagaagacaa cctcagccgc gtccaagaca tcatccacga attggaagaa
    80161 cagttaactc ctttggccgc ccagagtgag gcagcaaagg agtttttacg gctaaaagaa
    80221 acactgactc aaaccgatgt ctctttgatg gttgctgaaa tcaagacagc aaaaaaagat
    80281 tgggacaaca aacaagcaca gcttgcgaaa ttcaatctag aattaggcaa actatctgag
    80341 tcgatccaag aacaagaatc cattttggcg aagcagcgta aagaaaatgc gcaagccgat
    80401 cgtctgatcg aaaaaaatca gcaagtatta ttagatcttt cggaaaaact gaagcagaca
    80461 gaaggacaaa aagatgtttt gcaggagcgg acaaagcata cacaaaaaag ctcgcaagaa
    80521 tatcaaactt cattagcaga agcacaaaaa aaggttaaac attttgaaaa gcttcaagaa
    80581 agcttgatga aagcagcagc agaaaaagaa acagagatcc aaaaagctga agcaaatctc
    80641 ataaaaacac agcaagaatt agaaaaatac caaaaatcaa cgaaagaatt attggcagaa
    80701 ttaagagatc aatatgttga tttgatgcaa gaacaagcag ctgtaggaaa cgaactgaag
    80761 tatctagaac gtcaatatat acaagaaaca gcaaaaagca agcagacgtt agccaagcaa
    80821 tcggaagtag aagcatcagt agaccgattg atcttacaaa aagaagaatt gacacaaaag
    80881 caggcgcagc taaaatcatc tttaactgaa acaaaagaga agttagaaat gatccaacaa
    80941 aacgggaaaa agttccaaga agcattagca aaagaacagc ctaaaatgta tcaattaatg
    81001 aatcaagtcc agcagctacg tgctcgtaag aaaagccttc aagaaatcca agaaaactat
    81061 tttggttttt accaaggggt tcgattggtc ttgcagcata aacagcagct ttcgggtatt
    81121 gtcggagcag tggctgaact gatcgatgtt cctgcagatt tcacgttagc tatcgaagca
    81181 gctttaggtg gggcagccca gcatgtcatc gttgaaaatg aaaaagatgc tcggcaagcc
    81241 atcacgtatt tgaaacagca aagaggagga agagcaacat tccttccact gacgacaatc
    81301 aaaccaagac aattaccggc tcatatattg acacaagcag cagctgtaga agggtttata
    81361 gggattgcca gtgaacaagt cagttatccc gatcaaatcc aaactgtggt tcaaaacctg
    81421 ctaggaacga tccttttagc caaagactta actagtgcca atgcaatcgc gcaaacgatc
    81481 cgctatcaat atcgagtggt ttccttagaa ggtgatgtga tgaatgccgg aggatccatg
    81541 acaggtggag cgaataaacg aggaaaccaa ggcagtttgt ttgtacaaaa tcaagaattg
    81601 aaacagttga ctagtgaatt tgaagaagca gataaacaac tccaagcaca agaaaaaaaa
    81661 gttcaggagc tgcaacaaga aacagctcgt ttagccgaag aacaagaagt tttgcgtaca
    81721 cgaggcgaac aattacgttt tgaagaacaa gaagcgacaa atcagctaca aaacatcata
    81781 aatgaacttg aacgttttga aaaagaaaag caaatctcaa cttttgagac acgtgaactg
    81841 cagcagttta tcgaagatta cgaaaagcaa acgaacgaat tgaaagacaa acaaacagat
    81901 cttgaatcgc aacgacaaaa aatcgacgaa gaaatcaaat cgttaagtca agaaagcgac
    81961 cagatggaag ccagaagagc acaagtacaa agtcaaaaag cacaagaaca agcagatctt
    82021 gctgtcttga aagagcagtt taatcatctg cagatccagc tccgcggtgc ccgagtccaa
    82081 aaagcagagg caaccgaacg tcaagaagcg atagaaaagc agttggcgac attgacagca
    82141 gacttttctg atcatgaagt cacagaagaa agtttggaaa aacaaattaa cgaattatct
    82201 gctcagcgag aaacattaaa agccgagttg gcaaaagcca aagagcagcg agatagaagg
    82261 caaaaggaga tcgataagct tgaagcagtc ttggcagagc gtaatcgtga gcaaaaagca
    82321 cgactgtcgg aacaatccaa attagaagtc caaaaagacc gagctgaaat gattttagat
    82381 aatcatttga cctatctgca gtcagaatat cagctgacat ttgagaaagc ttcccaagac
    82441 tatcaagaaa caaccgatat cgaagacagt agaacaaagg tttcatcctt aaaagaacag
    82501 attgaaaaac tgggaccagt aaacttaaat tcaatcgaac aatacgaaca agtcagtgaa
    82561 agacatacat tcttagcaac acagcgagat gatctgttag cagcgaaaaa ccaattgttt
    82621 gaaacgatgg atgagatgga tgatgaagtg cggacaagat tcaaagaagt atttgaagct
    82681 atccgtcaag aattcaaaat tgtctttcca aatatgtttg gcggcggtaa agctgaatta
    82741 gtactgacgg aaccaactga tttgctgaaa acaggtattg agattgaagt acaacctcct
    82801 ggcaaaaaac tgcaaagtct aagtttgctt tctggaggag aacgagcact tacagccatc
    82861 gcgttgctct tttccattat ccgtgtctgt cctgttccgt tttgtatctt agatgaggta
    82921 gaagcagcgt tagatgaagc caacgtgaaa cgttttggtc gttacttgag cgatttccaa
    82981 gatgacacac aatttatcgt agtcacacac cgcaaaggaa cgatggtcgc agcagatgta
    83041 ctgtacggag tgacgatgca ggaatccggt gtatcaaaaa tcgtttctgt acggatggaa
    83101 gatatcaatg aagaggcgaa aatcaacgaa acgaaagtgt aggaacgaaa atgatcaaat
    83161 tagtagcaat cgatttggac ggtacattat taaatgaaca aaagctgatt tcagatgaga
    83221 ataaacaagc attagcacaa gcaaaacagc aaggggtcaa gatcgttcta tgtacaggac
    83281 gccccttagc tgcgatggcg cattatctgc aagaattagg actggtagat gaaggtgact
    83341 acagcatcac tttcaacggc ggtttggtac aaaaaaatga tactggtgaa atcatggaaa
    83401 agaaagtgat ggaagtatca gatatccaac gcttatacaa actggctcag caattggatc
    83461 tgccactaga tgttttgtct gacaatgtgg ttttacagct gcctagtgcc ccaaggaagc
    83521 aatccttgta caatgtgttg aataagctgc tgcaattcca accagcggat ataagcgata
    83581 ttacagatga atttgtgctt aataaggcag taatcgctta tgaccaagaa gaattagatc
    83641 ctaaaatcaa agaaatacca gcagaattcc atgaaatgta tgagatcatc aaaacaagaa
    83701 gtatgctttt ggaatttatg ccaaaaggtg ttacaaaagc ttatggtatt tctttattgg
    83761 caaaagatct tggtttgaaa caagaagaaa tcatggcgat cggtgatgag gaaaatgatt
    83821 taccgatgat ccaatatgct ggactaggcg tagcgatgga gaatgctgtg cctttcgtca
    83881 aagaagcagc agatttcgtg acagccagca atgaagaaaa tggcgtagcc aaagtgatcc
    83941 aaaaatttgt attagagtag ggggagtcaa atgggatttt ttgataaaat caagcgtgct
    84001 tttactggcg aagagaaaac agaagaagtt gaagtacagg agaagtacga caagggttta
    84061 gaaaagacgc gtaaaacatt tggtgaacgg atgaatgagt tgtttgccaa ttttcgaaca
    84121 gtcgatgaag acttttttga agaattagaa gaaacactta tcggtgcaga tgtaggtttt
    84181 gaaacagcga tacgaatcac cgaagcatta cgtcaagaag tgaaactcag aaatgcgaaa
    84241 aaatcggcag aagtccaaaa tgcgatcatc gagaaacttg tggatctcta tgaagcggaa
    84301 ggaatcaatg aagtaaatga actaaatatc caagaagacg gactaacagt tattttgttt
    84361 gtcggtgtta atggtgtagg gaaaacaaca agtattggta aacttgccca tcagttcaaa
    84421 aacgaaggta aaaaagtatt gatggcagca gcagatacct tccgcgctgg agcaatcgac
    84481 cagttagttg tctggggcga acgcgctggt gttgaagttg ttcgtggcaa tgcaggaggc
    84541 gatccagcag ctgtcgtatt cgatgctttg gaaagagcaa agacagagca ggcagatgtt
    84601 ttactagttg atactgcagg ccgactgcaa aataaagtga acttgatgaa cgaattagaa
    84661 aaaataaaac gagtaatcca gcgggaatta ccagaagcac cccaagaaac acttcttgta
    84721 ttagatgcaa ccactggtca aaacgcgatg gttcaagcaa aacaattcaa agaaacaaca
    84781 gatgtgaccg gtcttgtttt gacaaaatta gatggtacag caaagggcgg tatcgtactt
    84841 gctatccgta atgaattgca tttaccagtg aaattggtcg gtcttggtga aggaatcgat
    84901 gacttggagc cgtttgatcc aaatgatttt gtcgttggat tgttcaaagg attactaaaa
    84961 gaagaataac ccatgtctaa aaagcaggag ctgtgacaaa agttgcagct ccttgtttga
    85021 atgattgttt ttaacatagc tgtgtttttg atgccttcat ttttatactt atttgtaagc
    85081 ggttctcaaa caatttcgtt ctttttctta gactttttga ttataattag attcatatga
    85141 attggaggtc acttaatgat gaattttaag caattattat tgcatgtcaa tgcgcgccct
    85201 tttatcgacc aagctcgatt tggtatagaa cgggaaggac agcgggttga tcttgcagga
    85261 aatctagcaa aaaccgatca tccagcaatc tttggcgatc gatcctatca cccctatatc
    85321 caaacagatt ttagcgaaac acaaacagag atgatcaccc ctgttaccga ttctattccc
    85381 gaattatttc agtatctcgc tgctgtttat gatgtgactg ctcgttctat accgaaagaa
    85441 gagatgatct ggccattaag tatgccacct gccttaccgg aaaaagacga agagatcatt
    85501 attgcaaaat taaaaaattt cgaagatgtc ttgtatcgac gttatttagc aaaagaatac
    85561 gggaagcgaa aacagatggt aagcggtatt catttcaatt ttgaatttgg cgatgagttg
    85621 ctaagaacat tgttcagcca tcaggaagaa tttcaagatt tttccgaatt caaaacagaa
    85681 ctttatttga aaacagcgag aaactttatg cgctatcgct ggatgatcac ttatttattc
    85741 ggtgcttcac caatgagtga gaagaactac tttttagatg aatcacatcc gcaagaacct
    85801 gttcgcagta ttcgcaacag tgcgctgggc tatacgaatc atccaaatgt gaaagtttcc
    85861 tatgcttcta tgaaacagta tttagcagat atcgagcgaa tgatcgaaga agggaaactt
    85921 tcggaggaga aagaatttta tacaccgctt cgtttccgag gcggaaagaa agtcgcagat
    85981 ttagcaacaa caggtgttcg ttatattgaa ttgcggaata tcgatttgaa tccttatgca
    86041 agattgggta tcaatccaga gcaagttcgg ttcttacaat tattcctgat gtacatgtta
    86101 tggacagaag aaaaagaaga ctgtgaccaa tgggtagcag aaggtacgac tcgaaataac
    86161 aaagtagcac tagaacaacc atcagatcaa acagaatttc atcaagaagg tagagaaatt
    86221 cttgaaggaa tgaagcaaat gctggttgaa ttggattggt tggattctct ttatttagta
    86281 gaggaagcat tgactcaaat ggatcatcct gaacaaacgt tagcagcgaa actctatcaa
    86341 gaagcacagc tatcgagtca gcaagaagtt gctgtggcat tgggacatca atattataaa
    86401 gaaagccatg aacgtcccta tcagttggcc ggttttcgcg aaatggaact ttctactcag
    86461 atatttatgt tcgatgccat ccaaaaaggt gtccaagtca aagtattaga tgagtcggat
    86521 caatttttgc gtctgcaatt ccaagaccat gtagaatatg tgaaaaatgc aaacatgaca
    86581 agtaaggaca gctatatcgt cccacttatc atggaaaaca aaacagtaac gaaaaaagtc
    86641 ttaaaagaag ctgggttccg ggtaccagga ggcgcagaat tttcatccat ggaagaagcg
    86701 gtaaaagctt atccgagatt tgccgagcag gcatttgtga tcaaacctaa atcaaccaat
    86761 tacgggttag gtatcacgat tttcaaggaa ggagctagtc tggaagatta tcaagcaggt
    86821 ttagccattg cttttcgtga agacagttcg gttttagtag aagaatttat gccgggaacg
    86881 gaatatcgtt tctttgtgat cgacggagaa gtccaagcca tcatgttgcg agtccctgct
    86941 aatgttattg gtgacagtat ccgaacagtg aaagaacttg ttgaagaaaa aaatagtgac
    87001 cctttgcgtg gaacgaatca tcgtgcacct ttagaattga tccaattagg tgaattagaa
    87061 caattgatgt tgaaagaaca agggttgacg atcgaatcag ttccccaagc caatcaaatt
    87121 gtttatctaa gggagaactc aaatatcagt acaggtggtg attcgatcga tatgactgat
    87181 gaattttctg aagcttacaa aaaaatcgcc gtttctgcgg tagaagcact aggagctaag
    87241 attagtggta tcgatttgat tataccagat aaagaaatcg atcctacaac tgataaaaaa
    87301 gcatatggga tcatcgaagc aaatttcaat ccagccatgc atatgcatgt ttatcctttt
    87361 gcaggaaaag gcagaagatt gacgatgaat gtattgaaat tgttatatcc tgaagtattt
    87421 taaaagaaaa agcttttgac agattgtcc
//
LOCUS       DAZUMO010000003        68198 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000003 DAZUMO010000000
VERSION     DAZUMO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.003"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..483)
                     /locus_tag="ACG8ER_000226"
     CDS             complement(40..483)
                     /locus_tag="ACG8ER_000226"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5048847.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(499..1659)
                     /locus_tag="ACG8ER_000227"
     CDS             complement(499..1659)
                     /locus_tag="ACG8ER_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5048848.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2166..3866
                     /locus_tag="ACG8ER_000228"
     CDS             2166..3866
                     /locus_tag="ACG8ER_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048849.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(3968..4801)
                     /locus_tag="ACG8ER_000229"
     CDS             complement(3968..4801)
                     /locus_tag="ACG8ER_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS5048850.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5105..7270)
                     /locus_tag="ACG8ER_000230"
     CDS             complement(5105..7270)
                     /locus_tag="ACG8ER_000230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS5048851.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKNPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7530..9824
                     /locus_tag="ACG8ER_000231"
     CDS             7530..9824
                     /locus_tag="ACG8ER_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS5048852.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9817..10482
                     /gene="pgmB"
                     /locus_tag="ACG8ER_000232"
     CDS             9817..10482
                     /gene="pgmB"
                     /locus_tag="ACG8ER_000232"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5048853.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10475..11482
                     /locus_tag="ACG8ER_000233"
     CDS             10475..11482
                     /locus_tag="ACG8ER_000233"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5048854.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11504..12523
                     /locus_tag="ACG8ER_000234"
     CDS             11504..12523
                     /locus_tag="ACG8ER_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5048855.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12804..14171)
                     /gene="nhaC"
                     /locus_tag="ACG8ER_000235"
     CDS             complement(12804..14171)
                     /gene="nhaC"
                     /locus_tag="ACG8ER_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5048856.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14280..15701)
                     /gene="tyrP"
                     /locus_tag="ACG8ER_000236"
     CDS             complement(14280..15701)
                     /gene="tyrP"
                     /locus_tag="ACG8ER_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS5048857.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(15944..17821)
                     /gene="tdc"
                     /locus_tag="ACG8ER_000237"
     CDS             complement(15944..17821)
                     /gene="tdc"
                     /locus_tag="ACG8ER_000237"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5048858.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18112..19368)
                     /gene="tyrS"
                     /locus_tag="ACG8ER_000238"
     CDS             complement(18112..19368)
                     /gene="tyrS"
                     /locus_tag="ACG8ER_000238"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS5048859.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(19480..19685)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19948..21249
                     /locus_tag="ACG8ER_000239"
     CDS             19948..21249
                     /locus_tag="ACG8ER_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5048860.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21318..21917)
                     /locus_tag="ACG8ER_000240"
     CDS             complement(21318..21917)
                     /locus_tag="ACG8ER_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5048861.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            22171..23052
                     /locus_tag="ACG8ER_000241"
     CDS             22171..23052
                     /locus_tag="ACG8ER_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5048862.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            23065..24675
                     /locus_tag="ACG8ER_000242"
     CDS             23065..24675
                     /locus_tag="ACG8ER_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5048863.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(24752..25411)
                     /locus_tag="ACG8ER_000243"
     CDS             complement(24752..25411)
                     /locus_tag="ACG8ER_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5048864.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(25694..26359)
                     /locus_tag="ACG8ER_000244"
     CDS             complement(25694..26359)
                     /locus_tag="ACG8ER_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS5048865.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(26506..27111)
                     /gene="dhaL"
                     /locus_tag="ACG8ER_000245"
     CDS             complement(26506..27111)
                     /gene="dhaL"
                     /locus_tag="ACG8ER_000245"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS5048866.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(27139..28149)
                     /gene="dhaK"
                     /locus_tag="ACG8ER_000246"
     CDS             complement(27139..28149)
                     /gene="dhaK"
                     /locus_tag="ACG8ER_000246"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS5048867.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(28124..28519)
                     /gene="dhaM"
                     /locus_tag="ACG8ER_000247"
     CDS             complement(28124..28519)
                     /gene="dhaM"
                     /locus_tag="ACG8ER_000247"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS5048868.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            28673..28993
                     /locus_tag="ACG8ER_000248"
     CDS             28673..28993
                     /locus_tag="ACG8ER_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS5048869.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(29105..29962)
                     /locus_tag="ACG8ER_000249"
     CDS             complement(29105..29962)
                     /locus_tag="ACG8ER_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5048870.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(30070..31374)
                     /locus_tag="ACG8ER_000250"
     CDS             complement(30070..31374)
                     /locus_tag="ACG8ER_000250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5048871.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(31371..31880)
                     /locus_tag="ACG8ER_000251"
     CDS             complement(31371..31880)
                     /locus_tag="ACG8ER_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048872.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(32055..34190)
                     /locus_tag="ACG8ER_000252"
     CDS             complement(32055..34190)
                     /locus_tag="ACG8ER_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5048873.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(34273..35928)
                     /locus_tag="ACG8ER_000253"
     CDS             complement(34273..35928)
                     /locus_tag="ACG8ER_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5048874.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(35930..36244)
                     /locus_tag="ACG8ER_000254"
     CDS             complement(35930..36244)
                     /locus_tag="ACG8ER_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5048875.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            36414..36674
                     /locus_tag="ACG8ER_000255"
     CDS             36414..36674
                     /locus_tag="ACG8ER_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS5048876.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            36688..37002
                     /locus_tag="ACG8ER_000256"
     CDS             36688..37002
                     /locus_tag="ACG8ER_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS5048877.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(37732..38286)
                     /locus_tag="ACG8ER_000257"
     CDS             complement(37732..38286)
                     /locus_tag="ACG8ER_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS5048878.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(38412..40341)
                     /locus_tag="ACG8ER_000258"
                     /pseudo
     CDS             complement(38412..40341)
                     /locus_tag="ACG8ER_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(40647..41363)
                     /locus_tag="ACG8ER_000259"
     CDS             complement(40647..41363)
                     /locus_tag="ACG8ER_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5048879.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(41364..43187)
                     /gene="glpO"
                     /locus_tag="ACG8ER_000260"
     CDS             complement(41364..43187)
                     /gene="glpO"
                     /locus_tag="ACG8ER_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS5048880.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(43201..44697)
                     /gene="glpK"
                     /locus_tag="ACG8ER_000261"
     CDS             complement(43201..44697)
                     /gene="glpK"
                     /locus_tag="ACG8ER_000261"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS5048881.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(44833..46293)
                     /locus_tag="ACG8ER_000262"
     CDS             complement(44833..46293)
                     /locus_tag="ACG8ER_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048882.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(46283..47578)
                     /locus_tag="ACG8ER_000263"
     CDS             complement(46283..47578)
                     /locus_tag="ACG8ER_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5048883.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(47910..48587)
                     /locus_tag="ACG8ER_000264"
     CDS             complement(47910..48587)
                     /locus_tag="ACG8ER_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS5048884.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(48617..49708)
                     /locus_tag="ACG8ER_000265"
     CDS             complement(48617..49708)
                     /locus_tag="ACG8ER_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS5048885.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(49809..50243)
                     /locus_tag="ACG8ER_000266"
     CDS             complement(49809..50243)
                     /locus_tag="ACG8ER_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5048886.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(50827..52284)
                     /locus_tag="ACG8ER_000267"
     CDS             complement(50827..52284)
                     /locus_tag="ACG8ER_000267"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5048887.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(52281..54176)
                     /locus_tag="ACG8ER_000268"
     CDS             complement(52281..54176)
                     /locus_tag="ACG8ER_000268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5048888.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(54234..56009)
                     /locus_tag="ACG8ER_000269"
     CDS             complement(54234..56009)
                     /locus_tag="ACG8ER_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5048889.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(56059..57555)
                     /locus_tag="ACG8ER_000270"
     CDS             complement(56059..57555)
                     /locus_tag="ACG8ER_000270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5048890.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(57749..59371)
                     /locus_tag="ACG8ER_000271"
     CDS             complement(57749..59371)
                     /locus_tag="ACG8ER_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5048891.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(59699..61012)
                     /locus_tag="ACG8ER_000272"
     CDS             complement(59699..61012)
                     /locus_tag="ACG8ER_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5048892.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(61257..61481)
                     /locus_tag="ACG8ER_000273"
     CDS             complement(61257..61481)
                     /locus_tag="ACG8ER_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048893.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(61501..61923)
                     /locus_tag="ACG8ER_000274"
     CDS             complement(61501..61923)
                     /locus_tag="ACG8ER_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5048894.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(62065..62775)
                     /locus_tag="ACG8ER_000275"
     CDS             complement(62065..62775)
                     /locus_tag="ACG8ER_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS5048895.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(62793..63290)
                     /locus_tag="ACG8ER_000276"
     CDS             complement(62793..63290)
                     /locus_tag="ACG8ER_000276"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5048896.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(63327..63779)
                     /locus_tag="ACG8ER_000277"
     CDS             complement(63327..63779)
                     /locus_tag="ACG8ER_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5048897.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(63914..64519)
                     /locus_tag="ACG8ER_000278"
     CDS             complement(63914..64519)
                     /locus_tag="ACG8ER_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5048898.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(64969..65589)
                     /locus_tag="ACG8ER_000279"
     CDS             complement(64969..65589)
                     /locus_tag="ACG8ER_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5048899.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(65591..66460)
                     /locus_tag="ACG8ER_000280"
     CDS             complement(65591..66460)
                     /locus_tag="ACG8ER_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5048900.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(66483..66890)
                     /locus_tag="ACG8ER_000281"
     CDS             complement(66483..66890)
                     /locus_tag="ACG8ER_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5048901.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(66909..68198)
                     /locus_tag="ACG8ER_000282"
     CDS             complement(66909..68198)
                     /locus_tag="ACG8ER_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5048902.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        19619 a        14442 c        11828 g        22309 t
ORIGIN      
        1 aaaaattagg ctgggagcat tatttcgtta ttctgttcat caaaaatcag cgttttgatt
       61 ccatttcatc acaagttcct cctcatttac tgcttcatct ttttgctgtg tttgaattgt
      121 aaaaccttgt gaaagataga aatgaatagc tgattgattt ttttgataga ctgtcaaagt
      181 cagtgtttta ttggtttctt ttattttttt cattagttgc gtaccgattc tttgttgccg
      241 gtattccctt ttcacaaaga ttcctgcgat aaactgatca atcaccccag caaaaccaat
      301 aatttcttga tctatttctg ctaagtaaat cactgcatct ggtaaagctt tcctaacaga
      361 ttctacttga ttctcccaat aagttgatgg aatgaattca tgagaatcca agttagcatg
      421 cagccaaatc gatagaacag attccagttc tttggcagac aattttgttg ttgtgcggat
      481 cattttttct ctccttactt attcattgat tttaaaataa aaaccatttt tttgaagctc
      541 ttttacaagt tttcgcccct caaaaaagta tatcttctct tgttttactt ttctaatacc
      601 aaaagaaaca gcaactttag gatcatcagc tgaaacgatt cgataagttc caccaggcaa
      661 ttgatccagc ttgactttcc catgatggtc gctttgtact gaaaacggca caccatttct
      721 agtaagtgtt tttttcttat gtttcaaaat gaatgaacta ccatttaccg gtttccctga
      781 cgaatcacta ataataaatg aaaaatcaag cactttttta cttaatctat actgatagat
      841 tagtcgtccg ataaacacta acagtatgac tacagctatt atcgttcctg caagaataaa
      901 ccattgccgc atggttcttt cctgatttcc ttttccttct tcttttttta ccgattcgtt
      961 ataaggaatc cgatgtcctg tcactagcaa acgatgcgtg ttgatcatat acggcgtaca
     1021 tgtaagcaag gtaactaagt cttctccttc tgaaatcgtt agaacagacg tgtcatctgg
     1081 cagtaccact tgtatatttt taacttgata agccaatttc tcccccaaaa cagtcaaaat
     1141 aaacgtgtct ccgttttcta aacgatctaa ttcggtaaat aaccgtcgat taggtaaacc
     1201 gctgtgagca gagatcactg aatgcgtcga atttccacca agcgggaaag aggttccttg
     1261 caagacgcct gctccatttt caaggatcct accattgagc cgatcataaa gtgaaatatt
     1321 taattggatc ttaggtatat tgatacttcc gatcagccat tcctctaatt ggctggatgt
     1381 tgtactttca tttctgttct tttcattgaa aggatcagta ccaggattga atccttggtc
     1441 agttaatcgt tggtttttct cctccatttt ctttcgctgc acttcaattt cagctgcggt
     1501 ccgtttttgt acttgttcga tacgtttttg atcaataaaa ctatttaaag aatccacata
     1561 aaatggatat aaagcgatca gtaaccctac gacgatacat attaataaaa acaaacgaaa
     1621 caaaagtcga cgagactgtt tttgcttact ctgcttcaca tgtgtcctcc cttgcaagtt
     1681 tttttgttct ttcttttatt atatgggttc aggaaaaaaa tagaaaatga aagccgatga
     1741 ggttgtgaaa aagctgtcct gcatcaagta ataagtacag cccaaaaaaa tagctactca
     1801 tattttttgt tgggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
     1861 agaggttgtg aaaactgcgt tatgctttga gtaataagag cgctagtcag aaaatagctt
     1921 ctcatatttt ctaggctagt gagcttatta ccgagaagca gctgattgaa caccgtttac
     1981 cgagaggtca tgaaaaagct actttgtttc gagtattaag aaaaattctg tttcaaaaca
     2041 aggagatgca acaaaactta tgtcgcatcc tctctcctta gaactttcac ttatgttttt
     2101 cttgtaaaat tcataaatat tttcatgaat tttctaattt cttgcctacc tctcgcatag
     2161 gatatatgat gaatttatct aaagtttgca aaggagttcg acaattgaaa aaatatggcc
     2221 catttttagc aatttttttc attgctttgg catttctcat gcctcattta ttcacacata
     2281 aactaatttt gggtgctgat gccccattcc attacaatcg gttctatgat gccgcgcaac
     2341 aaattaaata tggggatttc cattattttc cttcccttta tggattccag caaagtggca
     2401 ggatcgtgaa tgccgtttac ggcccatttt tcgcttattt acaaggattg atcgtgttgc
     2461 ttgcaggtag ctggtttcat tatcagattc tttcaaattt cattctttat cttatttcag
     2521 gattttctat gctgatttta ctgagatatg tcaatgttcg aaattgggta agtgtaccag
     2581 tagccgtttt attcatgagt acgtattcta ttcaatactg gtctatcaac caaggattca
     2641 ctagctgggg gacttgtttt tatcctttat gtatgatccc tattgctgat ttcgtccttc
     2701 ataaaaagtt ccctgttatc aaagtcgctg tctcagtggg acttatgaca cagatccatt
     2761 tactgtcttc gatcatgtta gctttgatgt atatcccttt ttatctttat tactttttta
     2821 accagactga aaaaggaaaa gcactgcttc aactgattaa gtcggtcttg ctttattttg
     2881 ttctgacagc aaatatttgg gcaggattag ctattgttta cagtgggaat aagattcttc
     2941 ctccctttgt taacagagaa atgagtcaaa aggcgataga tctggatggc agttattggc
     3001 tccattaccc taaggccttt cctatattga tattatcagg ttttttgctt tttattttct
     3061 ttcaacaaaa gaacacctta ttccaaaaaa tcgtgttgta cacaagtctt gttttctttt
     3121 ttctttctac tagcttgatt ccatggacga cattgatcga aaaaaatgtg ccttttatta
     3181 gtttgatcca gtttccattt cgatttttcg tgccatttac gctattgctt ctactttacc
     3241 ttgccctttc actgaaccag tggtctgaac aaaagtggtt ccgtctttta agtatatttg
     3301 gcattcttat tttttcagca cagacgatcc agaacttata ccagcatctg gaaaagtggg
     3361 aaaatgaaac atttgtcagt cgacatactt atctattcga tacgccggaa gaagcaagga
     3421 aaacattctt cagcaaagat atgtctacca gcttgttcgt tttccaaaaa accacacctg
     3481 actatctgcc aatctataag gagacaaaag acaataaata cgaccggtat aacgaagaaa
     3541 tactagaaaa cgaaacaact tttataaaaa cacatccagg cggtcttttg acaatcaagt
     3601 ggaaaaacac tggggacaaa actgttcata tccctgttat cgtctacgat cggactgtgc
     3661 tacagcaaaa tggaaaaaca ttgactgatt atgaagtaac agatatcggg accccaatcg
     3721 tgaagcagca aaaaggaatc aacgagctca cgcttcatta tcaaacgcca atctatttct
     3781 atttcattct ttctcttact ttgattggct ggtttacctt gctatgttta tttatttatc
     3841 atagatacaa gcttttacgt gcttaggcaa aagggataaa tacatccgat caaaaaactc
     3901 tccaaacggt ataatcgttt ggagagtttt attttaatca gtttttttat gttatatgtt
     3961 atatttttta ttcgttcaca tctaattcga tttccacccc gtagtgatca ctgatgattg
     4021 gactatcgat cccatcaaaa atccgttgat attttttaac ctgcattcct tcagggacga
     4081 aaataaaatc tatacgcaat ttttctgtat tttgttccca accatctatt ttcttttcca
     4141 cagtagctgc ttttcctttt tcattcgcga caacatacga atcttgaaga agaaggcaac
     4201 tttctcttac agtataataa ccttgagagt ctactggatt attgaaatca cctaggaaaa
     4261 aaagtggtac gcgagtttcc aaaaagtagt tttccaagtt tttccactca aaataaaagc
     4321 cacttttttt atctgtccac caagaaaagt ggcagctcga gacaaaaatg tcctgatcat
     4381 ctatctttgt tttcccaatc aagatcttgc gtgtctgata atcttctttc ttttgaaatt
     4441 gggagacggt aaggacctga ctttccaatg tttttttcgt aagcagtgca tttccttctt
     4501 catatttatc ataaccgatg tggctcattt cccaactcca aaaatactct tttccgtgtt
     4561 cctttaaaag ctgaactaaa cggtaggcaa aattatcttt acggataggt acttgattct
     4621 ttaccggaca gaacttcatt agcttcgcag gttctacttc ttcagattcc agtaattggt
     4681 tgatttcttg taaagcaatc acttcataat cttctaacag gatcttatct gctaattctt
     4741 gcaatttttt ctctggttct ggctcaagcc agctatgcgt gttgagtgtc atgaatttca
     4801 ttggttttct ccttgtcttc atttcgaaac aaaagggttg tgacaaaatt tttgtcacaa
     4861 cctctccagc aaatagtgtt ctcctgctaa ctcctcagta ataagctcaa tggaccagga
     4921 gctatttttt gtttggctgt ttttatcact tggtgcaggg cagctttttc acgacctctc
     4981 cactaaacag tattcaaact gctgcttctc ggtaataagc ccaaccaaac gaaaaatagg
     5041 aggagctatt tttgtttggc tgttcttatg acttgatgca ggacagcttt ttcacaacct
     5101 ctccttacat tttaatggag ccgatcactt taccagctgt cactgtacct aattgcttga
     5161 tcactacttc ttctacttta tcgtttgtaa atacgatcat gatcgttgga tctttttctt
     5221 cgttttggat cacatcaaga tccatctcag ctaatttcgt tcctgttcct acttcttggc
     5281 cttctttgac agcaatctca aaagctggct gattcatttg gactgtatcg attcccatat
     5341 ggatcaaaac ttcgatacca tctttcgttt ccagcccaat tgcatgtttg ctagggaaaa
     5401 tacttgtcac ttttccagcg atcggcgcaa caacttctct tgatgttgga ataacagcaa
     5461 agccgtcacc catcattttt tgtgagaata ccggattttt tacttcagat aaaggaatca
     5521 cttctccaga agctgcggaa actagttctt gttctatacc taagtattgc ttactacctg
     5581 cttgtccatt tcctgtagaa accatttctg gctctggaat ctctgctcct gattgcagca
     5641 gatcttcgat atctgatttc aagacatctg cttttggtcc ataaactgct tggactccat
     5701 tgtctttgac gatcaatccc atcgcccctg ctcgtttcca ggcttcttca gtccctactt
     5761 tttctcgatc atttacactt acgcgtaacc gagtcataca agcatcgaca tctttgatat
     5821 tttcttttcc tcccagcaaa tgaatgatct ggaccacttg ctgatctgct gttcctgttc
     5881 cgctgttaga tacaacggtt tcttcactgt cattgtcgta atttccgtta cgtcccggtg
     5941 tagcaaagtt gaattttttg atcaagaaat ttgctaagaa atacgtaaca acaccaaaag
     6001 cgatcacaca caacacgaag ttgataagat ctccacctag acccgctttg atagctaacg
     6061 gtgtccgagt cagcaattcg atattcccaa atgaatggac ccgcaatggc aagatatctg
     6121 ccatcgcaaa tgcagcccct tggatcactg aataaatgac atataatggc acagcagcaa
     6181 acataaacat gaactccaat ggttctgtta cacctgttag aaagacagcc aacgctgctg
     6241 aaaaatacat tgatttatat cttgctttct tatcaggatc gacattacga tacatcgcaa
     6301 aagccattcc catcaagata cctgaagaac caatcatttg tcctactttg aaacgagccg
     6361 gcgtccagtt atgcaacaca aactcatatt gcgacatatc ccctgcacct ttaagattca
     6421 ctaaatcagt ggcccaagcc aaccataacg gatcttgccc aaatacttgt gtccccgcct
     6481 gtgcgcctga taaaatctca tacgatccgc caagctgtgt atagttgata ggaatcgtca
     6541 gcatatgatg caatccaaat ggcaatagta aacgttctaa tgttccaaat aaaaacggtg
     6601 ctagaatcgg tgcgctttct tgtgattctg caatccataa accaaaatta ttgattccag
     6661 cctgtacgtt tggccacacc acagcaagaa tcaatgccac gattgttgac cataagataa
     6721 caacaaacgg aacaaagcgc tttccattga aaaatgacaa agcatccggc aattttctat
     6781 aattatagta cttattataa gccattgccc cgacaaatcc agcgatgatc cctacgaata
     6841 ctcccatatt caaagctggt gcttccaata cacttgtaaa aaatccttta accatgatct
     6901 tcgtaccgaa cagagtgtgg gtaaatgcat cttcattcgc aagcatgtca cttgtgacac
     6961 caaagatagc cccagtgatc cgattgatta aaataaatga gatcccagct gcaaatgctc
     7021 cgcctgctct ttcttttgcc caacttcctc ctattgctaa agcaaataaa atatgaagat
     7081 taccaataat tgcccaacca atattttcaa ttactccgcc cgttgtgaca agcggcgtcc
     7141 aatcaggatt gatcaaggga attgatttcc ctatactgat catcaaccca gctgccggca
     7201 taactgctac cacaaccatt aaagcctttc caaatttttg ccaaaactca aaactaaata
     7261 tctttttcat tccgtttagc tcctttacgc aatcggtttc gttaaacgta ttatagcgtg
     7321 cgttttcttt tttgtaaatg ctttattttt tatttttctg tctataccat tctataaaac
     7381 ctataatacg tgtgttccct tctataattt tctcctaaaa atttattttt ttaagctttt
     7441 ttctatgaaa tattttcttt tgtctattga cttgtgcaat cggttgcgtt tattatgaac
     7501 gtataaaaca gctcattagg aggagaaaaa tgaaacagat caaacgacta tttgctattg
     7561 atccatggaa aatcagcacg acacaattag ataaaaaaaa tcttcgactg caagaatctt
     7621 taacaagtat tggaaacggt tatatgggga tgcgcgggaa ctttgaagaa acttactcag
     7681 gggaccacca tcaaggtact tatttggcgg gtgtctggta tcccgataag acacgtgtcg
     7741 gctggtggaa aaatggttat ccagaatact ttggaaaagt gatcaacgcc ttgaacttta
     7801 tcgcgataga tatttcgatc aacgatcagc tgatcgactt ggcttctttg gaaccagaag
     7861 acttttactg ggaattagat atgaaaaatg gtgtattatc tcgtacattc acaatcacta
     7921 cttctactaa taaagtacgc atttccttcg aacgttttct gagcattgtc aaaaaagaat
     7981 cagcttatat ctatttaact atagaaatgc ttgaaggtag cggaacagtc aaactcgtat
     8041 caaaattgga tggaaatgtt caaaacgaag acagcaacta tgatgaacat ttttggaatg
     8101 agattgatcg tggaactata gaagaaatcg gctatttgac gactaaaact attccaaaca
     8161 actttgggat tgaacaattt actgttactg cagcaatgcg ccatttcgtt aatggaaaat
     8221 cagtcatccc tgactatcac gaagatactt tagcattaca tggttgcttg tctgcagaat
     8281 tatctcaagg tgagacatgg tcagtggaaa aagaaattat cgttcttact agtagagata
     8341 ttccagaagc gcagcaagca gaaaaagctg tctcagaatt aacgaaccag acaaacaatt
     8401 atatccaagc aaaagaagag caaacagcgg cttgggcaga acgatggaag cttgccgatg
     8461 tggtcatcga aggagacgaa gaagcacaac aaggtattcg attcaatttg ttccaactat
     8521 tctctactta ctacggcgaa gatgaacgat tgaatatcgg ccccaaagga ttcacaggag
     8581 aaaaatacgg cggagccact tactgggata cagaagcgta tgctgtccct ctttatcttg
     8641 ctttagccaa acctgaagta actaagaatc tgctgaaata tcggcataat caactgccgc
     8701 aagcaatcca caacgctcag caacaaggat taaaaggcgc gctttatcct atggtcacct
     8761 tcactggtgt cgaatgccat aacgagtggg aaatcacatt cgaggagatt caccgaaatg
     8821 gcgcgattgc ctatgcaatc tacaattata cgaattacac cggcgatacg gaatatttga
     8881 aaaatgaagg actggaagtt ttagcagaaa tcgcacgctt ctgggcagac cgtgtccatt
     8941 tttcaaaacg agcaaataaa tacatgatcc acggtgtgac cggcccaaac gaatatgaga
     9001 acaatatcaa taacaattgg tatacaaata cgatcgcagc ttgggtcttg cgttacacgc
     9061 gagaaaatta tttaacttat caaaaagaag ctgctatcac tatcagcgaa gaagaactag
     9121 ccaaatggaa agatatcgca gagaacatgt acttcccagt tgacgaagaa ttaggaatct
     9181 ttgtccagca tgacacattc ttagataaag atctcatgcc tgtatccgat attcccacaa
     9241 gcgatcttcc tctgaatcag cattggtctt gggacaaaat ccttagatct tgcttcatca
     9301 agcaggcaga tgtactacag gggatttatt tcttcaatga tacttttaca aaagaagaaa
     9361 agcaaaaaaa ctttgatttc tatgaaccaa tgactgtcca tgagtcttcc ctttcgccaa
     9421 gtatccattc gattttagca gcagaattag gaatggaaag caaagctgta gaaatgtacc
     9481 agcgaacagc tcgcttggat ttggataatt acaataacga cacagaagac ggcttgcaca
     9541 tcacttccat gactggtagc tggttgtcga ttgtccaagg atttgctcaa atgaaaactg
     9601 cccatgatca gttgagattc gcaccatttc tgccaaaaac ttggaaagct tattctttcc
     9661 atatcaatta tcgcggacgt ttattgttca tctcagtcac agatgaacaa gttactttcg
     9721 agttattaga aggttctcct ctagaaattt acgtctatga acagtcttat tctttagcgg
     9781 atcgtctaga aataacgcta agaaaggagg aagcaaatgt ttaaaggggt attgtttgat
     9841 ttagacggtg tcattaccga tactgctgaa ttccattatc gtgcttggcg aaagcttggt
     9901 caggaaatcg gcattacgat cgaccacaca ttcaacgaac agctaaaagg cgtcagccga
     9961 gaagactcac tctctttgct tttagcttat ggcggaaaag aacactcttt ttcaaaggaa
    10021 gaatttgcag aattagcgaa aagaaaaaac gattattatt tagagatgat ccagacgatc
    10081 gaaccaaaag atgtcttccc cggggtcgtg cctttactgg attctttaaa agaagaaaaa
    10141 atcaagattg ctcttgcttc tgcaagtaaa aacgggccat tcctacttga aaaaatgggt
    10201 cttacacctt actttgatgc tattgccgac ccggcagaag caacaaacgg aaaacctgcg
    10261 ccagatatct tcctccttgc agcaaaagct gtcggactaa aagcagaaga atgtatcggg
    10321 atcgaagacg cgcaagccgg tatccaagcc atcctctcaa gtggcgctca accaattggc
    10381 gtgggacgtg ctgaagacct tggagaggag atcccgcttg tccctgatac gacttttctt
    10441 acgatcgatt acttgaaaga aaagtggcat gaccatggat aatccagaga tctccgtcgt
    10501 accagttcca gagactgaaa taaagaaaat cacattgaag aacgactact tagaagtcgt
    10561 tcttctcaat tacggtgccc gcctgcacca aatcttcgca ccagacaaag aaggaaaatc
    10621 agaaaatgtt ttattgtctt acgatcgttt cgaagatgtt ctaacagaca aaagcttttt
    10681 tggagcaact gtcggacctg tagccggaag gatccgggat gcttcttggg gtgagcatag
    10741 attggagaaa aactgcggat cacaccatat ccacggtggc acgaaaggct ggtctttcca
    10801 attttgggaa gtagaagttt tcaaaactcc atcatcaata ggtgtggtct tttacttaaa
    10861 agatgaattt tctacctatc caggaccaat cactgctacg atcacttacc gtttaacaaa
    10921 aaacgaatta gagatgacaa cctctgctag tagccttgtt gaaaccatct gcaatccgac
    10981 caaccatgcc tacttcaacc tttcaggaaa tggaacaaga gacatctatg aacatcagtt
    11041 aacggtcttt ttagatggtg tgcttgaatt agatcaagaa aaactgccta ctggaaattg
    11101 gacaaaaaaa gaaaaactgc caatcgactt tagaaaatcc ccaacactgc aagagatcct
    11161 tgcatgttat ccagatggac tagatgatgt atttcttttg catcatccta ggctttccaa
    11221 aacttcctta caattattcg agaagcattc tggtcgccaa atgactatag caacttctaa
    11281 taaaagtatg gtattatttt ctacgactgg attcgaagct gatttctctg tcaatggaaa
    11341 acagatgcat tcaaactacg gattagcaat cgaaccgcaa gaaatccctg atatcgttca
    11401 tttccccaaa tttggatcga tcaacctcca tcctggtcaa gaacggatca gtcagacgat
    11461 ttatcggttc tctgcacaat aacaagagaa aggtgttttt atcatggcta ttaccgtaaa
    11521 agatgtggcg aaaaaagccg gtgttgccac ctcgaccgtc tctcgtgtca tcaacgatca
    11581 tcctagtatt tccgagacaa caaagaagaa agtccgaaaa attatggatg aactaggtta
    11641 tgttcctaac atgactgccc gcaatctagg aaaacgcatt tccagtgcca tcggcgtaat
    11701 cttaccccct cttgattcaa aagagcggat cggtaaccca ttttatcttg agatcatgga
    11761 agcaatcaac gaagaagcgc ggaattatga tatgactaca gcaatcgcta ctgctaaaaa
    11821 ttttgaattg ctgttagaga acgtccagcg tatgcattta caaaaacaag tagatggttt
    11881 tattctagtg tattcagata gtcaagatac tgtcattgat tatttatacc aaaaccacat
    11941 ccctttcact ttgatcggac agccttatca tcatgagaac gaaatcgttt atgtagataa
    12001 tgataaccag cttctaggta aacaagcaac agaatttttg atccagaatg gacataagaa
    12061 tatcctgttt gtgacaaaca cgactcatga aagtttgtat ttcgaacgat atttcggtta
    12121 tcaaaaagca atgatgatgg ctggtcttcc tgcgttatcc agtgtagtac tagagcatcc
    12181 ggaggattat ctgtcctttg agaaaacgtt gaaagaaaac gaagccacag cattggtggt
    12241 cattgacgat atcttcgccc ttcgcacgat gcagttggcg caaatgtatg gctatgtcat
    12301 tccagagaca ctctccgtca ttagcttcaa caactcgatc ttctcaacat taacacaccc
    12361 ttaccttaca agtatcgata tcgatatctc cgaattagga cgtgtcgcta tgcaaaagct
    12421 gcgcgaattg atccatgaag aattgagcag tggcgtacgt ttggtcatcc ctcatcgctt
    12481 gatcaaaaga gagacagtcg ttcctttaga aaacccatac taaatagctg actcactaaa
    12541 tacgctgacc atttatccta tggtcagctt tttatgtaaa agaagatact acttctaaca
    12601 cattgtttat ctgtaaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
    12661 ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc tgaaattcac
    12721 aaaaatttaa aaagcaattt tcttgagttt cttcttattt cttggagtta aacgcttctg
    12781 tcccagcctc ttttttcctt ccttcaattt tttacacgtc gacgattcaa taaaaaaccg
    12841 cctaatattg taaagacagg tgcaagaatc gagaaaaagg cgaatggcaa gtaatgcaac
    12901 gcgctaactt tcatcgttcc cataatgaat gtgccgctga ctccccacgg tacgagagaa
    12961 ttgacggtcg cacctgaatc cgctagtgtt cgtgtcaaat aggtttttga taagccaacg
    13021 tcatcaaaag aacgcttgaa tgtttctcct ggaagaatga tggacagata ctgttctcca
    13081 acgatcagat tgattccgat acagctcaag gcagtaaacc cgactaaacg agaaggtcgg
    13141 tccatctttt cttttaattc ttgaacgatg gtatcaacga tcagatattt gatcagtaac
    13201 ccacctaatg ccaatgcaag taaaatcaaa ctagcagaac ctaacatact catgatcccg
    13261 cctcgagaaa gcagttcatc tagctttgga tcaggtgtat ttgcgacata accattcatc
    13321 aagatgtctg ctgcttttcc gaccgtcaaa cttttctcat ggataaaaga aagaacgaga
    13381 gcagctgtcg atcccgctag taatgtaggg atagccggaa tttttttgat tgccccccca
    13441 aataaaataa ctagtggaat caatgtccaa ggagagatcc aaaagctcgc tttcatgttt
    13501 tggatcattt cattgatact agctagatcg gctgaatgac tgttatgtcc taatatcgta
    13561 taaaaaatca acgaaaggac gaatgctggg aaatccgtat aacgcatacc agcgatatgt
    13621 tcgaatagat taactcctcc aatacccgct gccaagttgg tcgtatctga taaaggcgag
    13681 cagttattcc ctaaaaaggc accggaaacg actgcgcctg tagtcattgc atcattgaat
    13741 ccaagtaaat gaccgatccc caaaaaagca attccaatcg tcgaaatcgt tgtaaatgaa
    13801 ctgccgacag tcactccgac aattgcacaa acaagaaatg ctgtcggtaa aaagaaacgc
    13861 acagaaacaa gtttaaatcc ataaaccata atcgtaggga tggttccaga taaaatccat
    13921 gaagcaacta agacaccaat caaaagaaaa atcaggattg gaatgatccc tggagtgatt
    13981 cctttgatga tcccgtcatg gatctcatcc catgtgaact ttttaaatct gccataaaac
    14041 aacaaacaca tgaaagctgc aaggatcggg atatgtggcg agagctggaa tccgatgatc
    14101 agtactccta ggagtgccaa caacacgatc aatacagcga tactttcttt taaagaaata
    14161 tttttattca tcgtcgatct cctatttcaa atgaaaaggc cctgccatga aaaaactatt
    14221 cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga cttatttttt
    14281 tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt gaagtgattg
    14341 atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa tgcatcactc
    14401 aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat cagcatgcag
    14461 ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg ttcgaattct
    14521 ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac gatacaataa
    14581 ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc cattacacca
    14641 taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt tctagatacg
    14701 ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc acgagctact
    14761 tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag cacaccaata
    14821 aataccatga agctgaagat atttccgatc acttctggta agcctaggat ttgacagtca
    14881 atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac gacatttgct
    14941 acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat aaatacgcct
    15001 tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact catttcgatc
    15061 ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg ttccaagtca
    15121 gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag tccgactttg
    15181 aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac ccctagccat
    15241 acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat cgtgatgatc
    15301 cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag ttctacatta
    15361 ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat acctgggaac
    15421 atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag tgctgttttt
    15481 acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt
    15541 gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg
    15601 actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt cagaccgata
    15661 aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac tctccttgat
    15721 tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa acaattcagc
    15781 gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa gacaacctca
    15841 aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa catgcgtcag
    15901 aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt gcttcgcttg
    15961 ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct ttgatttttt
    16021 cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg ctggcacgca
    16081 atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt tggttcacaa
    16141 attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa gttaagaact
    16201 cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca tggttcaatt
    16261 tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct accatattga
    16321 aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta aatgacaagt
    16381 cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt aatttaccat
    16441 atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct gctgttgccc
    16501 ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct gcgccttttt
    16561 caaatacata tgtcgcaaag taagagataa cgtttctcat gcggatatct ttgatgacga
    16621 taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt acagattctg
    16681 cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct ttgttttctg
    16741 taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg aagttgttgt
    16801 cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg tgtaagtaga
    16861 agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg ataccgtcga
    16921 ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt gttttttcag
    16981 ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa
    17041 ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct tttagccatg
    17101 aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt tttccgctac
    17161 gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac agattcatga
    17221 tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact agttcaggtg
    17281 tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa agtccttcta
    17341 agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag ctcattaatt
    17401 ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa gattcatagg
    17461 caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat ggcatcaatg
    17521 tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg gaatgggtac
    17581 gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg tgttcaaaac
    17641 tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg tagttttgac
    17701 gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt tggccgttct
    17761 cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat gattcactca
    17821 tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta cacctgttct
    17881 tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat gtatcaatta
    17941 tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac aaatgtaaac
    18001 tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg gtttcaaaat
    18061 tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat tctaatcaat
    18121 tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact taccatcgaa
    18181 ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt taatacttat
    18241 agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt ttgtggaaac
    18301 aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca ttttaccgaa
    18361 tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca atgcttgcgt
    18421 tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa cttcttctgc
    18481 caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg ctaagtcttc
    18541 gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat cgcgatcatc
    18601 ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat caagccaaac
    18661 ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt
    18721 taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta atccagctgt
    18781 gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg ttttatgcag
    18841 atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg agattcctac
    18901 ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga agtttttacc
    18961 gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa
    19021 tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg cgtccacgtt
    19081 atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc
    19141 gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca attggaatct
    19201 tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac
    19261 gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt
    19321 ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt tactgacctc
    19381 cgtgtagttt ttttatgaaa gcaggaacct gctagcatat gtaattaatt atttcttcag
    19441 gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga aatctcgcgg
    19501 taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta acgctgtcgc
    19561 gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg tactggtttg
    19621 cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc gatcttcgtc
    19681 ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca acagaaaaag
    19741 gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt ccgaacattt
    19801 tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca atttatcgag
    19861 aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg gacaagtaaa
    19921 aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc
    19981 acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct
    20041 acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca
    20101 gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac
    20161 caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt
    20221 cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc
    20281 tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag
    20341 gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa
    20401 ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa agacgaacaa
    20461 ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca
    20521 caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc
    20581 gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt
    20641 gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat
    20701 cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca
    20761 tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa
    20821 gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg
    20881 aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa
    20941 gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc
    21001 gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc
    21061 gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc
    21121 tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa
    21181 aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa
    21241 tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt
    21301 ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc
    21361 cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc
    21421 attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct
    21481 ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc
    21541 atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa
    21601 aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt
    21661 tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata
    21721 aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc
    21781 gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg
    21841 tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc
    21901 ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata atatatatag
    21961 ttcaaattta ttctgaggtt tattataact atctattgag aaagtatcaa gctagcaatt
    22021 ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata
    22081 aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta
    22141 tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg
    22201 aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt
    22261 gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt
    22321 ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa
    22381 gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga
    22441 aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct
    22501 caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac
    22561 tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat
    22621 ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa
    22681 gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc
    22741 agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac actcgatgaa
    22801 gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa
    22861 gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac
    22921 ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa
    22981 ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat
    23041 tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt
    23101 tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct
    23161 ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga
    23221 ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg
    23281 aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt
    23341 ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa
    23401 cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct
    23461 gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg
    23521 atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg gctatctgta
    23581 ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt
    23641 tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca
    23701 ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg
    23761 acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta
    23821 atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc
    23881 cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta
    23941 ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg
    24001 gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa
    24061 cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggta
    24121 gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa
    24181 gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat
    24241 tggaccaata tgttactagc tattctattt gctatattgg caatcctgat tagttctggt
    24301 gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg
    24361 tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc
    24421 tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt agcttactcc
    24481 tttgcactca attactttgg cggaattttg gatttaccag aatggttttc caaaacagct
    24541 gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg
    24601 ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac
    24661 ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca
    24721 aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa
    24781 tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt
    24841 ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg
    24901 gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc
    24961 gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct
    25021 caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga
    25081 aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact
    25141 catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta
    25201 aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga
    25261 tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat
    25321 gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa
    25381 caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc
    25441 taataaagaa acataacttc ccttattcca actgataatt ttcttttatc atagcataaa
    25501 cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt
    25561 tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg
    25621 aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct
    25681 tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt
    25741 cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata
    25801 gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg
    25861 tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc
    25921 atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact
    25981 ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat
    26041 agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag
    26101 attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt
    26161 cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa
    26221 tctctttcca tttttgccag tgttctttat attctttctt gatttgttca atcttatctg
    26281 ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat
    26341 ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat
    26401 tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca
    26461 gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat
    26521 cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc
    26581 gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg
    26641 ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac
    26701 tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat
    26761 acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac
    26821 ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc
    26881 accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg
    26941 atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg
    27001 gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc
    27061 ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc
    27121 cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt
    27181 ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt
    27241 cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa
    27301 aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag
    27361 tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt
    27421 tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc
    27481 aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac
    27541 agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag
    27601 ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac
    27661 aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat
    27721 atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc
    27781 catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc
    27841 actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc
    27901 accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg
    27961 tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac
    28021 acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat
    28081 ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc catcctttct
    28141 ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga
    28201 tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct
    28261 gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg
    28321 caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg
    28381 ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt
    28441 tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa
    28501 gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt
    28561 tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg gaaaatatgt
    28621 gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac
    28681 aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg
    28741 tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt
    28801 tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc
    28861 cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga
    28921 ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt
    28981 ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt
    29041 ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc
    29101 ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg
    29161 ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg
    29221 atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt
    29281 tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt
    29341 tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt
    29401 gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa
    29461 cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt
    29521 ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat
    29581 ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca
    29641 atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt
    29701 gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg
    29761 aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac
    29821 cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca ggttcagaag
    29881 tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta
    29941 acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt
    30001 attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg
    30061 gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa
    30121 aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa
    30181 caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac
    30241 aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa
    30301 actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat aatgcccgat
    30361 taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt
    30421 ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt
    30481 gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt gtcgatatag
    30541 catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa aaacagataa
    30601 ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc gatcttgtct
    30661 gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc
    30721 ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa
    30781 ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt
    30841 gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc
    30901 atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc
    30961 aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag
    31021 attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc
    31081 gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt
    31141 atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac
    31201 actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca cataacacca
    31261 gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaaatacat
    31321 aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga tcatttcagt
    31381 tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt tttctcttcg
    31441 gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg ttccgtatac
    31501 acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc aaaagattta
    31561 tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga ggtaatcagg
    31621 aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt cttttctgat
    31681 aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg gaaaaagacc
    31741 catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt ttgccaaatc
    31801 tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga tgcaacttct
    31861 tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg taggagttta
    31921 ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag aaaaagaaca
    31981 aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg ttctttttat
    32041 tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat tcctctagat
    32101 atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc aaatggatat
    32161 aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat ttggcagttt
    32221 tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc ctcttctgtt
    32281 tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc tagtattttc
    32341 aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg cggtcggatc
    32401 gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc tgtgattgct
    32461 ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg gtagtcgtcg
    32521 aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg attctcgtca
    32581 cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa ttgtgccagg
    32641 gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt taccttttca
    32701 acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg tgtgggttgc
    32761 caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg atgctcatcc
    32821 acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg taaaagtttc
    32881 cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc gaataattcc
    32941 aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact ttttgcttgt
    33001 ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg aagcctatcg
    33061 atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt tttagaaagg
    33121 actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa aaacaaagga
    33181 tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt ctgctccgat
    33241 acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc ctctgctgaa
    33301 agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc taatgatggt
    33361 aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag caagtcatcg
    33421 gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat gatagcagag
    33481 gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac atcccgaatg
    33541 attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt ggtctcatct
    33601 tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc aaaaaacttg
    33661 accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt atcgatttta
    33721 ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag ttcggcaatt
    33781 ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt tgctgtaccg
    33841 atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt tccaaagtag
    33901 ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt ctgtttgata
    33961 ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc caggttatct
    34021 aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga ctggtttcct
    34081 tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat tagcttttga
    34141 tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat acacattctc
    34201 ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg aatgcaagct
    34261 ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag tttgatataa
    34321 agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga ttctttccgc
    34381 tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc tgtctaattc
    34441 atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga atttgccctt
    34501 ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta gttctgtcgg
    34561 caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg ctgcatatcc
    34621 tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca attctaattc
    34681 cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat cgatccgttt
    34741 atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct ctggatggaa
    34801 gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat cttttcctga
    34861 tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac ttaaaccggt
    34921 ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc cttgatttct
    34981 tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg ctggtgaagc
    35041 aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag catcccccac
    35101 cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac ccccgcgtat
    35161 gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac caatggataa
    35221 aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt tcccgtggtc
    35281 ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag acaattcttt
    35341 ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg cttgttcgat
    35401 caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc cgatgaatcc
    35461 tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca ctttatcgat
    35521 atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg gaatcgatgg
    35581 aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa tgtgtttttg
    35641 accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca cttcatgatt
    35701 tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga caagaagatt
    35761 ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg caaaagaaac
    35821 atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc gaagccgtgt
    35881 agcagcagac attccacctg ctacacctcc gacaataacg attttcatct tatttccctc
    35941 ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca tcataaccag
    36001 atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa atcacataga
    36061 cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca tttttagctc
    36121 cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac gctggatttt
    36181 ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga aatagcaaat
    36241 tcattttctg cacctcattt acatttttat aaatacctca tggggtatat taaacaggag
    36301 ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt tatatttgca
    36361 taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt gttatggaga
    36421 ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt cgtggtatcc
    36481 aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta agcgctgttc
    36541 gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa aattgctttg
    36601 agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg atcaagatga
    36661 ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga tgaattagaa
    36721 aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc agaaacgacg
    36781 attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc caatgcagaa
    36841 ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg tgctaatcat
    36901 atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt ccttgattta
    36961 gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga ggttgcgaca
    37021 gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt ctggaaaacc
    37081 gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag ccaaacaaaa
    37141 atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    37201 ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag aaaaaaacgg
    37261 gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa
    37321 caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt
    37381 gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa actggctttt
    37441 gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagaaaca
    37501 aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa ggtcagctga
    37561 ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc acaagaaata
    37621 atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag aagtagctac
    37681 tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt tttattttct
    37741 ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa gaccgtaagt
    37801 gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa gcagcaaaag
    37861 gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg ctccacttaa
    37921 tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt cttttatcaa
    37981 gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca aaatcagaaa
    38041 aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg ttacctgatt
    38101 atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa caatgaccgc
    38161 tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca gcaaaaaaat
    38221 acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt ctgtgcttaa
    38281 tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc ttacacgggg
    38341 aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata ttatgagcta
    38401 atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg tcgtttgcgc
    38461 atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc acgctgccgt
    38521 ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg gtacagatct
    38581 tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt tttgtctaaa
    38641 tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt ttggtattca
    38701 ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt ctccttccta
    38761 tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc tttttccgcc
    38821 aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg agcaatccac
    38881 aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac gaagtgcttc
    38941 ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga atttcttgat
    39001 ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag attccatgat
    39061 tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct catctaaaag
    39121 taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca tatgaccaga
    39181 aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga aaatctcatc
    39241 aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca tcgccatcaa
    39301 agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg ctccggtgat
    39361 aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat gctcaggtaa
    39421 aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc taggtgtgct
    39481 tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca ccgtgatcgt
    39541 ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca atgccgccag
    39601 gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt cattcaattt
    39661 agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat cgataatcgt
    39721 tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt cgcctttttg
    39781 aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga atttcagtaa
    39841 ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac gcaaattacg
    39901 tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact cttctacatt
    39961 tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt attgattctt
    40021 atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt cgatcaaatc
    40081 ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga tcctcggcaa
    40141 ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc ttgtttcttc
    40201 ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga ttcgtttttt
    40261 gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa attgtaattg
    40321 actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg tcccaaaata
    40381 aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc atgtcctatc
    40441 cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag cacaccaaat
    40501 aaactttgtt tattggctat tcgacttact gattagatac cagctacact cacaaaacat
    40561 accccattct tctaggtctt atttattttc cacttaacta tagcaagaca cattttggtt
    40621 gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc taccgcgata
    40681 attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc tgaagttcct
    40741 ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg agcagggttg
    40801 attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa tccacctaca
    40861 gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa cccaagaact
    40921 aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg tatagcagga
    40981 cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca atgtggcaaa
    41041 taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc aatgatgaaa
    41101 ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga gactgcagga
    41161 ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac agcgagtccc
    41221 caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt taaattcaca
    41281 gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa ctcccccatg
    41341 atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag atctgattct
    41401 gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt ccattcaaaa
    41461 tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc tcgttcaaac
    41521 aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat ttcttcttct
    41581 aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg tgccatttct
    41641 tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat ataagtagca
    41701 tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac tgtttcttct
    41761 aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc gatttctttc
    41821 gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc tccttctgcc
    41881 atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc tggttctctt
    41941 tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct gttttccaaa
    42001 tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata ttcacttact
    42061 gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc accattgtaa
    42121 tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc ttcgatatca
    42181 ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag ataatctaca
    42241 tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc tgtcgtacca
    42301 aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg ctttcctgta
    42361 tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa atggatccct
    42421 ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg tactttatct
    42481 acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac ttcatctgtc
    42541 agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa gccaaccgct
    42601 ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt atcaatcact
    42661 aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag cccttctttt
    42721 ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt ctcatatttg
    42781 gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac cgagaacata
    42841 ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc tggttttggg
    42901 atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc tactacttcc
    42961 acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga gcgagaggaa
    43021 gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga tgctgctgac
    43081 tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc caattcttct
    43141 tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg gatttcctcc
    43201 ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa gtttttctcg
    43261 gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt cgcggatctc
    43321 gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc ctagcgccgt
    43381 tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt ggaattgcat
    43441 cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct tgattccagt
    43501 gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact gtaaagtagc
    43561 tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc ctcgtgctgc
    43621 ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa cctcatcttc
    43681 gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca tttgaagtcc
    43741 atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta aagcatagta
    43801 aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg ataattgcgg
    43861 tttttcaccg gtattcatga caataaatga acctgtacca taagtattct tgaccatgcc
    43921 tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca tccctgaaat
    43981 tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt ctgagttaga
    44041 agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct cctgatccca
    44101 atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat cagtaacatg
    44161 tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa acatcaattc
    44221 gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc gtatcttcgt
    44281 tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt cttgatagcc
    44341 ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa caactgcatg
    44401 atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct ggttcgttat
    44461 gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc cggcaataac
    44521 tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt cttgcgggaa
    44581 atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt caaagataat
    44641 tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt ctgtcattgt
    44701 gattccctcc attatatgca aacgctttct ttaactactc ttatcatact actgttttaa
    44761 aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt ttattatgaa
    44821 gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt tcaatcgaat
    44881 aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt tgtttctctg
    44941 gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa tctcaatacc
    45001 gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat aaatcggcag
    45061 actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga tcatcaaaag
    45121 aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc ttttcctcgg
    45181 caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg aatgcccgag
    45241 cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct ttccaacaaa
    45301 gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt cttctttgat
    45361 tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag tatctaaaac
    45421 agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt cttcgtctac
    45481 tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat tcagttctgc
    45541 tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa gccggtccga
    45601 ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat tcaatagtct
    45661 ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg taaacgttgt
    45721 ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat tttgcaaaaa
    45781 aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat atcgtatctg
    45841 tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct ctaagagctg
    45901 gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg ctgtcgctaa
    45961 tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa acttcaagct
    46021 ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca acacaagcag
    46081 cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc caatcaattc
    46141 aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct ctctcaaacc
    46201 attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga tctgtaactg
    46261 ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc tttgaccaca
    46321 ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct aactgttcaa
    46381 acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac atctcttttt
    46441 tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat ttgagtttaa
    46501 ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca attttttggg
    46561 gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag ccaaaccact
    46621 gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta attgtcttgc
    46681 tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt ttttcatttg
    46741 ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt tgagacgttt
    46801 gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt tcttcccaca
    46861 tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc tcctttcttt
    46921 tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt tcttctaaaa
    46981 aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt tcaggatcta
    47041 gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt tttcttgatg
    47101 ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca attttctttg
    47161 cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact gggtgaataa
    47221 gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct agaatcaaac
    47281 gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc aatcctatta
    47341 ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct ttcgtgatcc
    47401 aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac ccgctgggaa
    47461 tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc atcaattggc
    47521 gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca atgttcatct
    47581 gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa acacagtatt
    47641 cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc ctctccatca
    47701 taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga agagtatcat
    47761 ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg ccttttacgt
    47821 acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg tgtgtaatgt
    47881 aatagttaga atagacacat catttatttt taattcttta atgcttcaac aactgcttcg
    47941 cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc aatttgaacc
    48001 acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa agcatcttct
    48061 gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt actgaaccca
    48121 gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg caacaatcct
    48181 acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg tgaagcgact
    48241 ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc agcataataa
    48301 accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc caaggcccga
    48361 tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt caaaacactt
    48421 tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc gatcccattt
    48481 tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt aggacgctcg
    48541 atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt cccatcctct
    48601 ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat gggcttggca
    48661 aataaagctc tttatatgct ttttcaaaag gtttcgttac aaattcatcc tcacggtttt
    48721 ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt gctgcaagtt
    48781 ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg acaccaacta
    48841 aaggaatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta tcgttgtatt
    48901 tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa tctagttttg
    48961 actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct ggtgtttcat
    49021 attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat tgttcaaccg
    49081 ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc aagctaccgc
    49141 cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc aaatacgtat
    49201 ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt gtggctttgc
    49261 caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt gtttccgcat
    49321 caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat aaagttgctc
    49381 gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct tctccttgga
    49441 ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg ctgagttcac
    49501 ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca gcaccaagac
    49561 ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca ttgaaaaagc
    49621 tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact cctgaaggta
    49681 aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc tctgattaac
    49741 ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac taatttgctt
    49801 aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg ggctaattct
    49861 ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc ccagctacca
    49921 tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga caagcgacct
    49981 tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg agcaatcgtt
    50041 gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc tttttctttt
    50101 aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa cgcttcatca
    50161 gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca ttcatctaac
    50221 gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc atagtatgat
    50281 ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag atttgaaaaa
    50341 aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct tctggaacac
    50401 cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa attttgtgaa
    50461 aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg cttttgaaca
    50521 ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga acagccaaac
    50581 aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca gctgattgta
    50641 caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag gagcaatttc
    50701 aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag ttggcaggag
    50761 aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt cccttatttc
    50821 cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat caaatgattt
    50881 tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat agatatatcc
    50941 ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc cccacgacgt
    51001 ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa tatgctgctt
    51061 caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt tgtctatagc
    51121 tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata attgattcaa
    51181 agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg gaagatacgg
    51241 atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt ctggatgagc
    51301 actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt aggctgctaa
    51361 gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa acattcgttg
    51421 cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag aagttctttg
    51481 ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa gaagctgata
    51541 gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt ttgtagctaa
    51601 agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca aatggggatg
    51661 gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt tgatctcatt
    51721 gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt cagcgaaacg
    51781 aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa ccaaatataa
    51841 cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta gacattcgtc
    51901 aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat caccatgagg
    51961 aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt cagcaaataa
    52021 acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag gataataata
    52081 cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat gccattgatc
    52141 tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca cagataatcc
    52201 tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc cgccccataa
    52261 aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt tcttgtttga
    52321 ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt aacaataacc
    52381 attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc catcaataaa
    52441 tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt gccatttaaa
    52501 ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact gactacccca
    52561 attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact gtagattttt
    52621 ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa tgttggatcg
    52681 tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac tatatccttt
    52741 gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat acaggaactg
    52801 atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat ataaatcggg
    52861 aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc accagctact
    52921 ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata tttaagagtc
    52981 acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc agataaagat
    53041 aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc tcctgcttgc
    53101 gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa agaaagattg
    53161 ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac ttgccaaaat
    53221 ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa atatccagct
    53281 gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat aattgggacc
    53341 atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg caagcttttg
    53401 cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg aataaccgta
    53461 cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat gctatcacta
    53521 tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc gatgaaacga
    53581 tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa atagaaaaat
    53641 gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa taaattcaaa
    53701 ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac gcctaaagtc
    53761 ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt tttctcagtt
    53821 cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa tacgtcatac
    53881 acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc gactacagtg
    53941 atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc ttttagggta
    54001 aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc tccgacaaat
    54061 cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt gttaattaat
    54121 gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt ctccattcaa
    54181 ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt tacttagccg
    54241 agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata gtcaaaggag
    54301 acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc aagctgatac
    54361 tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt ctcggcattt
    54421 aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga atctatcttt
    54481 ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt tgttttcttt
    54541 ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca agattcaaac
    54601 tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag ctcttgctca
    54661 gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa aagatacact
    54721 ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg tttttttagt
    54781 gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg ttttcgaaaa
    54841 ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa attgggacgt
    54901 aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg ataacgggag
    54961 atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct cgttttcatt
    55021 tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac aggaagatct
    55081 aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc tttgtttttt
    55141 tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc ttgtttttct
    55201 tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt ctctaccatc
    55261 ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg aagaatggtc
    55321 aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata attatatgat
    55381 ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa aattgtactg
    55441 atttgttgag gaaaccttgc gtgttggatc accgtttctg ttccaacaag agataagaaa
    55501 attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga aacttccagt
    55561 tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa tgatacggct
    55621 gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc tttttgaaac
    55681 aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat tttatgtttc
    55741 gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa aaaataacct
    55801 gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat atttttgatt
    55861 tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc gcttgttatc
    55921 caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt taagtccatt
    55981 tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg acaatagtca
    56041 cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt taccattaga
    56101 ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa agcttccgct
    56161 cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt cccctgttcc
    56221 tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc aaccatcttt
    56281 tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt ttggatcgtg
    56341 gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa ctatgaataa
    56401 tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta gaggatcaat
    56461 tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga aaccatttgc
    56521 tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac gataataact
    56581 aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat ctaacattac
    56641 tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat gccctcggca
    56701 catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa atacattctc
    56761 tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa tacccatctt
    56821 agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta gaaacatatg
    56881 atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa gtttatctct
    56941 agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat tattgatctc
    57001 attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg tctgcgcata
    57061 aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc ctaccaatgc
    57121 taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc gatttaatgt
    57181 attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt cgtctcctct
    57241 tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta actccttcag
    57301 taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag aaagataatg
    57361 gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact gaatactagg
    57421 atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc cgtcttccaa
    57481 ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat tttctggaaa
    57541 tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt gaagtatagc
    57601 tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa aaaattgaat
    57661 gctactcttc atttttaata tttatatata acaaatacat aagacgatcc ccgaaagaaa
    57721 acactcgtta accatctaag caaacgattt attttacttc caacacaaat gcttcataag
    57781 gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac acaataaccc
    57841 ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta caaataacga
    57901 gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc gtcttttcca
    57961 ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg atcaatttct
    58021 tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg atttctttat
    58081 aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt tcacgggcgt
    58141 tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat tccaagattt
    58201 cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc tcaatatctt
    58261 ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct ccttgataga
    58321 tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca ctttcttttc
    58381 gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga ttattccaaa
    58441 ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat acttttttca
    58501 gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc tgatctaatc
    58561 ctatgtgttc gaattggaag accatggata attcatttct tttaggattg gaataaagtt
    58621 tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt ttgtcaccaa
    58681 aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta cttgtgattt
    58741 ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc ccaatacctt
    58801 tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta tttttccaat
    58861 tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt ttttcatcca
    58921 actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt ccattgaccg
    58981 gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg gcctcaatga
    59041 accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata cggatatttc
    59101 ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat tctgccatga
    59161 ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg tatactggag
    59221 aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa ataatccccc
    59281 gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag atttgataaa
    59341 caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg gatgtttcta
    59401 ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc tttcaaatag
    59461 ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg aaattccctc
    59521 aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac aacttcttta
    59581 taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa aatataagaa
    59641 actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca caacctcttt
    59701 atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca tacatgattg
    59761 gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag ataaaaccta
    59821 atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc aagaatgaag
    59881 ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc atcaatactc
    59941 ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt aatggagaaa
    60001 agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct gaacgtcctc
    60061 caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta ccaaataagg
    60121 aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga ggcatcttgt
    60181 tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct gtatcaaaga
    60241 aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg atgtccccta
    60301 catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca acaatttcag
    60361 aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc atcccgatga
    60421 agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat ccaaaaatcg
    60481 tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat tcattcgaaa
    60541 cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata ccgccagaaa
    60601 tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc ttgaagattg
    60661 tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt tcccatggaa
    60721 tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga gcaggtgccg
    60781 ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca ctggctaatg
    60841 cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca gttggattta
    60901 caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc tcacgtttta
    60961 ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc atcttccatc
    61021 cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt aataaaacaa
    61081 cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga ttttaagcaa
    61141 aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac attcactttg
    61201 gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct caaaaatcaa
    61261 tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc ttttgtatcg
    61321 acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc ccagcctttt
    61381 gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg ttttgctcgc
    61441 cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc tcctttttat
    61501 tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt cagaaaaatt
    61561 gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt tctcgtagat
    61621 agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata cctctggtga
    61681 aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg agatgctgag
    61741 tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg caccaatttt
    61801 ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca cacgtgtttt
    61861 ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat aaactgattc
    61921 cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa aataaagcaa
    61981 tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac ggcaatgaat
    62041 gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt
    62101 gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc
    62161 ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg
    62221 tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc gacagatcgc
    62281 tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc
    62341 taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt
    62401 ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc
    62461 tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc
    62521 aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc
    62581 aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc
    62641 ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc
    62701 atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac
    62761 tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag
    62821 tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt
    62881 ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt
    62941 ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga
    63001 tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc
    63061 tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc
    63121 actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa
    63181 gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta
    63241 aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt
    63301 tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa
    63361 ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct
    63421 tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg
    63481 aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct
    63541 ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc
    63601 gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg
    63661 caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata
    63721 ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt
    63781 tcatcccacc tttattttgt ttgtaagact agttcacctc tttttgttgc aaatgcaact
    63841 attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct
    63901 tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt
    63961 tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt
    64021 tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa
    64081 caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg
    64141 ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat
    64201 tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc
    64261 gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga acatctactt
    64321 ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt
    64381 ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat
    64441 caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt
    64501 gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc
    64561 aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa
    64621 agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg
    64681 gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta
    64741 ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta
    64801 aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag
    64861 ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg
    64921 gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc
    64981 attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt
    65041 tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat
    65101 ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc
    65161 accatagtga taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt
    65221 gtaccgacgt tgccataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata
    65281 tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc
    65341 ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg
    65401 ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc
    65461 tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg
    65521 tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata
    65581 ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa
    65641 tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag
    65701 acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa
    65761 gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt
    65821 ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt
    65881 tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag
    65941 caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg
    66001 tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa
    66061 gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca
    66121 ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt
    66181 cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa
    66241 ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt
    66301 tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta
    66361 gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact
    66421 cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct
    66481 ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg
    66541 cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg
    66601 ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc
    66661 ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact
    66721 tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat
    66781 tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt
    66841 gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct
    66901 actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg
    66961 gctactgtat ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt aaagatcgtt
    67021 actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct
    67081 cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg
    67141 gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata
    67201 atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag
    67261 aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga
    67321 aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc
    67381 actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat
    67441 tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg
    67501 accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct
    67561 tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat
    67621 aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga
    67681 atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg
    67741 accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt
    67801 gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc
    67861 aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc
    67921 aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga
    67981 aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg
    68041 gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct
    68101 gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga
    68161 gtcaaaatat tttcgatgat aaaattgata acgcccat
//
LOCUS       DAZUMO010000004        61534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000004 DAZUMO010000000
VERSION     DAZUMO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.004"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8ER_000283"
     CDS             complement(59..187)
                     /locus_tag="ACG8ER_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5048903.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8ER_000284"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8ER_000284"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5048904.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8ER_000285"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8ER_000285"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5048905.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8ER_000286"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8ER_000286"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS5048906.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8ER_000287"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8ER_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS5048907.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8ER_000288"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8ER_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS5048908.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8ER_000289"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8ER_000289"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS5048909.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8ER_000290"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8ER_000290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS5048910.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8ER_000291"
     CDS             10491..12050
                     /locus_tag="ACG8ER_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS5048911.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8ER_000292"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8ER_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5048912.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8ER_000293"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8ER_000293"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5048913.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8ER_000294"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8ER_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5048914.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8ER_000295"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8ER_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048915.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8ER_000296"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8ER_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048916.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8ER_000297"
     CDS             17616..17771
                     /locus_tag="ACG8ER_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS5048917.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8ER_000298"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8ER_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS5048918.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8ER_000299"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8ER_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS5048919.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8ER_000300"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8ER_000300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS5048920.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8ER_000301"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8ER_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048921.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8ER_000302"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8ER_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5048922.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8ER_000303"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8ER_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5048923.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8ER_000304"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8ER_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048924.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8ER_000305"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8ER_000305"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS5048925.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERYSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8ER_000306"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8ER_000306"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS5048926.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACG8ER_000307"
     CDS             complement(28200..29369)
                     /locus_tag="ACG8ER_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS5048927.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8ER_000308"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8ER_000308"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS5048928.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8ER_000309"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8ER_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS5048929.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="ACG8ER_000310"
     CDS             34320..34841
                     /locus_tag="ACG8ER_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS5048930.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="ACG8ER_000311"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="ACG8ER_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="ACG8ER_000312"
     CDS             complement(36284..36940)
                     /locus_tag="ACG8ER_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048931.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8ER_000313"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8ER_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS5048932.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8ER_000314"
     CDS             complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8ER_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS5048933.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8ER_000315"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8ER_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS5048934.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="ACG8ER_000316"
     CDS             complement(40491..41222)
                     /locus_tag="ACG8ER_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS5048935.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="ACG8ER_000317"
     CDS             complement(41226..42491)
                     /locus_tag="ACG8ER_000317"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS5048936.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8ER_000318"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8ER_000318"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS5048937.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8ER_000319"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8ER_000319"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS5048938.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="ACG8ER_000320"
     CDS             47833..49335
                     /locus_tag="ACG8ER_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS5048939.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="ACG8ER_000321"
     CDS             49337..50074
                     /locus_tag="ACG8ER_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5048940.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8ER_000322"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8ER_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS5048941.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8ER_000323"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8ER_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS5048942.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8ER_000324"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8ER_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS5048943.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8ER_000325"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8ER_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS5048944.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8ER_000326"
     CDS             complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8ER_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS5048945.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKYGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="ACG8ER_000327"
     CDS             complement(56756..56983)
                     /locus_tag="ACG8ER_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS5048946.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8ER_000328"
     CDS             complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8ER_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5048947.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            complement(57969..58415)
                     /locus_tag="ACG8ER_000329"
     CDS             complement(57969..58415)
                     /locus_tag="ACG8ER_000329"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5048948.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            complement(58396..58992)
                     /locus_tag="ACG8ER_000330"
     CDS             complement(58396..58992)
                     /locus_tag="ACG8ER_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS5048949.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            complement(59476..59637)
                     /locus_tag="ACG8ER_000331"
     CDS             complement(59476..59637)
                     /locus_tag="ACG8ER_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048950.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            complement(59630..60304)
                     /locus_tag="ACG8ER_000332"
                     /pseudo
     CDS             complement(59630..60304)
                     /locus_tag="ACG8ER_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            60827..61434
                     /locus_tag="ACG8ER_000333"
                     /pseudo
     CDS             60827..61434
                     /locus_tag="ACG8ER_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
BASE COUNT        17079 a        13211 c        10644 g        20600 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aatctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttattcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatacttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt ttttaagtta
    59401 caataattat ctatttaata gtcttaattc attaaaataa aaaactcaac tttacaagga
    59461 tatggtttaa aaaatttatt tatccaaccc aactagtttt cgctgcttac gatttgcttc
    59521 atctgctacg taattcatta ctgttttaaa ttctttcgct gaggcgtttt ctgataaatc
    59581 agcgatactt ttattatact tcttcacgat ttgttgtact tgttctttct cagtcatgta
    59641 attcctcctc caactctttt gaagtatatt gtattggtag tactaattga atattatctg
    59701 ctgcataatg cacttcagaa atgtcgtttt gataatcctt aatcattatg tgaaggacta
    59761 cttcaggatg tctatttttg actttttcaa tattctctct ggacttaaaa aacgcattga
    59821 taaacatttc ttttggcaca tgacgtcctt cagctagttc acgttttcga gtgaaatccc
    59881 aggcaacttt gggatcttgg taaacataga aaatcgtaac gcgaaagtta tttttaaggg
    59941 ctcgaataat atttttctct gcactaagga tagcaaaagt atcctctagt atgaaagaat
    60001 agccatgttc cagtacatat tttaatgatt gttcaactaa ccaagaagaa gctttctgaa
    60061 aaacacttga gttctttcca ttgtattctg gaaattgtgc tctaaaatag tccgcatcaa
    60121 tcacaacatg gttgtcataa ttttaagcta aacctagtgc aacctctgtt ttacctgctc
    60181 ctgggcttcc agccatgaat accgcatctt tttcatcatc agggattttt cccttgatga
    60241 ttgtttcaat aaatttatct ttattgaccc tagcgtaatc taaatacttg tctttaggtt
    60301 ccaagattgc accttctttc cttcttctat attcatacta attatacgat atttatataa
    60361 aaaatataac ggtcatttta accatattaa catttagtca cagtaatagc agatatgctc
    60421 gcaaaatgag atatgaatgt acgcagaatc cttgttttct acggcattcg cttttttttc
    60481 attggactag ttatatattt ctagggttgt tttcatgtct gagtgcccga tatgatttat
    60541 ttttcgtttc tataattact ttttgccttc cagtttcttt taagattgta ctcaagcaag
    60601 tgggaattgg ttcttttgtc atccatgtat gcaaaatgtt ataatgctgg taaacaaaaa
    60661 tgattacctg tcatttttat ttagttcgta tttaattaag ttgactgtct gtatactctg
    60721 tctaaaactt aagaattgtt gttttcgctg tcgctatcca aatttaatga atgtctgtat
    60781 gttaaaactg ttgtccttgt catagatagt aaaggagagc ttgaaaatga aaactgcgat
    60841 tacgtttcta ttagaggaat atgccgattg ggaaggtgct tatattacta gcacactaaa
    60901 ccaaaaagag ggatggcaaa acaaaactgc ttctatccaa tcagaggtca cttccattgg
    60961 aggattaaaa acaaaaatcg attacttaat cgaacaaata cctgaaaaag ttgacctttt
    61021 tattatgatc ggtggcaatt catgggacat aaaaaatcat catttatatc aaataatcga
    61081 taaattgtta aaaacagacg taccagtagc tgctatttgt ggggcagttg attatttagc
    61141 tcagagcgga ttattgcaag gttacaaaca caccggaaat gcgcaatttc cttggttaga
    61201 atcggaaaat tatcagaata aagaagattt tatagaacaa caagctgtga aagatcgtaa
    61261 tttgattact gcgaatggaa cagctcctat agaattcaca gacttgattc ttcaagcagt
    61321 ccattttgaa acagaagaaa taagagaaaa aaagattcgt ctatacaaac ttggattcta
    61381 tgattactat aaaaagtacg gagatccttt ctcctaatca ttctcttcaa ttaaatactc
    61441 cttatcaata gtatgacaag taattttact gatggttatt tgtcacactt tttctgacat
    61501 ttatcaaaga agatacactc tatttatatt cata
//
LOCUS       DAZUMO010000005        60022 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000005 DAZUMO010000000
VERSION     DAZUMO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.005"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..537)
                     /locus_tag="ACG8ER_000334"
     CDS             complement(<1..537)
                     /locus_tag="ACG8ER_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048951.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKI"
     gene            503..2140
                     /locus_tag="ACG8ER_000335"
     CDS             503..2140
                     /locus_tag="ACG8ER_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048952.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2285..3025)
                     /gene="truA"
                     /locus_tag="ACG8ER_000336"
     CDS             complement(2285..3025)
                     /gene="truA"
                     /locus_tag="ACG8ER_000336"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5048953.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3216..3851)
                     /locus_tag="ACG8ER_000337"
     CDS             complement(3216..3851)
                     /locus_tag="ACG8ER_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5048954.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3998..5218)
                     /gene="sstT"
                     /locus_tag="ACG8ER_000338"
     CDS             complement(3998..5218)
                     /gene="sstT"
                     /locus_tag="ACG8ER_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS5048955.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5437..6435
                     /locus_tag="ACG8ER_000339"
     CDS             5437..6435
                     /locus_tag="ACG8ER_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048956.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6631..8466
                     /locus_tag="ACG8ER_000340"
     CDS             6631..8466
                     /locus_tag="ACG8ER_000340"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5048957.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8526..9242)
                     /locus_tag="ACG8ER_000341"
     CDS             complement(8526..9242)
                     /locus_tag="ACG8ER_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5048958.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9469..10011)
                     /locus_tag="ACG8ER_000342"
     CDS             complement(9469..10011)
                     /locus_tag="ACG8ER_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS5048959.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(10016..10243)
                     /locus_tag="ACG8ER_000343"
     CDS             complement(10016..10243)
                     /locus_tag="ACG8ER_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5048960.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10322..12202)
                     /locus_tag="ACG8ER_000344"
     CDS             complement(10322..12202)
                     /locus_tag="ACG8ER_000344"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5048961.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12391..13005)
                     /locus_tag="ACG8ER_000345"
     CDS             complement(12391..13005)
                     /locus_tag="ACG8ER_000345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5048962.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13147..13707
                     /locus_tag="ACG8ER_000346"
     CDS             13147..13707
                     /locus_tag="ACG8ER_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5048963.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(14012..14326)
                     /locus_tag="ACG8ER_000347"
                     /pseudo
     CDS             complement(14012..14326)
                     /locus_tag="ACG8ER_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14580..16215)
                     /locus_tag="ACG8ER_000348"
                     /pseudo
     CDS             complement(14580..16215)
                     /locus_tag="ACG8ER_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16538..17017)
                     /gene="rlmH"
                     /locus_tag="ACG8ER_000349"
     CDS             complement(16538..17017)
                     /gene="rlmH"
                     /locus_tag="ACG8ER_000349"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS5048964.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            17445..18734
                     /locus_tag="ACG8ER_000350"
     CDS             17445..18734
                     /locus_tag="ACG8ER_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS5048965.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(18773..19378)
                     /gene="ytpR"
                     /locus_tag="ACG8ER_000351"
     CDS             complement(18773..19378)
                     /gene="ytpR"
                     /locus_tag="ACG8ER_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS5048966.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(19583..20047)
                     /locus_tag="ACG8ER_000352"
     CDS             complement(19583..20047)
                     /locus_tag="ACG8ER_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5048967.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(20069..20389)
                     /locus_tag="ACG8ER_000353"
     CDS             complement(20069..20389)
                     /locus_tag="ACG8ER_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5048968.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(20533..21612)
                     /gene="pepA"
                     /locus_tag="ACG8ER_000354"
     CDS             complement(20533..21612)
                     /gene="pepA"
                     /locus_tag="ACG8ER_000354"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS5048969.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            21812..22162
                     /locus_tag="ACG8ER_000355"
     CDS             21812..22162
                     /locus_tag="ACG8ER_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5048970.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            22385..23332
                     /locus_tag="ACG8ER_000356"
     CDS             22385..23332
                     /locus_tag="ACG8ER_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5048971.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(23389..24807)
                     /gene="pepV"
                     /locus_tag="ACG8ER_000357"
     CDS             complement(23389..24807)
                     /gene="pepV"
                     /locus_tag="ACG8ER_000357"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS5048972.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            24956..25801
                     /locus_tag="ACG8ER_000358"
     CDS             24956..25801
                     /locus_tag="ACG8ER_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS5048973.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            25805..26245
                     /locus_tag="ACG8ER_000359"
     CDS             25805..26245
                     /locus_tag="ACG8ER_000359"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS5048974.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            26295..26489
                     /locus_tag="ACG8ER_000360"
     CDS             26295..26489
                     /locus_tag="ACG8ER_000360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048975.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            26625..27310
                     /locus_tag="ACG8ER_000361"
                     /pseudo
     CDS             26625..27310
                     /locus_tag="ACG8ER_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(27718..28407)
                     /locus_tag="ACG8ER_000362"
     CDS             complement(27718..28407)
                     /locus_tag="ACG8ER_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048976.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(28382..30518)
                     /locus_tag="ACG8ER_000363"
                     /pseudo
     CDS             complement(28382..30518)
                     /locus_tag="ACG8ER_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
     gene            complement(30566..31903)
                     /locus_tag="ACG8ER_000364"
     CDS             complement(30566..31903)
                     /locus_tag="ACG8ER_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS5048977.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(32155..32514)
                     /gene="rplT"
                     /locus_tag="ACG8ER_000365"
     CDS             complement(32155..32514)
                     /gene="rplT"
                     /locus_tag="ACG8ER_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS5048978.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(32567..32767)
                     /gene="rpmI"
                     /locus_tag="ACG8ER_000366"
     CDS             complement(32567..32767)
                     /gene="rpmI"
                     /locus_tag="ACG8ER_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS5048979.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(32839..33363)
                     /gene="infC"
                     /locus_tag="ACG8ER_000367"
     CDS             complement(32839..33363)
                     /gene="infC"
                     /locus_tag="ACG8ER_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS5048980.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(33407..33532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            33685..34317
                     /locus_tag="ACG8ER_000368"
     CDS             33685..34317
                     /locus_tag="ACG8ER_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5048981.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(34319..35224)
                     /locus_tag="ACG8ER_000369"
     CDS             complement(34319..35224)
                     /locus_tag="ACG8ER_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5048982.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(35309..35968)
                     /locus_tag="ACG8ER_000370"
     CDS             complement(35309..35968)
                     /locus_tag="ACG8ER_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5048983.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            36284..37228
                     /gene="mvk"
                     /locus_tag="ACG8ER_000371"
     CDS             36284..37228
                     /gene="mvk"
                     /locus_tag="ACG8ER_000371"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS5048984.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            37221..38198
                     /gene="mvaD"
                     /locus_tag="ACG8ER_000372"
     CDS             37221..38198
                     /gene="mvaD"
                     /locus_tag="ACG8ER_000372"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS5048985.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            38211..39296
                     /locus_tag="ACG8ER_000373"
     CDS             38211..39296
                     /locus_tag="ACG8ER_000373"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS5048986.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            39293..40357
                     /gene="fni"
                     /locus_tag="ACG8ER_000374"
     CDS             39293..40357
                     /gene="fni"
                     /locus_tag="ACG8ER_000374"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS5048987.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(40606..41409)
                     /locus_tag="ACG8ER_000375"
     CDS             complement(40606..41409)
                     /locus_tag="ACG8ER_000375"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS5048988.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(41429..42271)
                     /gene="kduI"
                     /locus_tag="ACG8ER_000376"
     CDS             complement(41429..42271)
                     /gene="kduI"
                     /locus_tag="ACG8ER_000376"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS5048989.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(42287..43294)
                     /locus_tag="ACG8ER_000377"
     CDS             complement(42287..43294)
                     /locus_tag="ACG8ER_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5048990.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(43301..43945)
                     /locus_tag="ACG8ER_000378"
     CDS             complement(43301..43945)
                     /locus_tag="ACG8ER_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5048991.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(44045..44803)
                     /locus_tag="ACG8ER_000379"
     CDS             complement(44045..44803)
                     /locus_tag="ACG8ER_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS5048992.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(44930..46081)
                     /gene="fucO"
                     /locus_tag="ACG8ER_000380"
     CDS             complement(44930..46081)
                     /gene="fucO"
                     /locus_tag="ACG8ER_000380"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS5048993.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(46163..46474)
                     /gene="rhaM"
                     /locus_tag="ACG8ER_000381"
     CDS             complement(46163..46474)
                     /gene="rhaM"
                     /locus_tag="ACG8ER_000381"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS5048994.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(46476..47321)
                     /gene="rhaD"
                     /locus_tag="ACG8ER_000382"
     CDS             complement(46476..47321)
                     /gene="rhaD"
                     /locus_tag="ACG8ER_000382"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS5048995.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(47335..48591)
                     /gene="rhaA"
                     /locus_tag="ACG8ER_000383"
     CDS             complement(47335..48591)
                     /gene="rhaA"
                     /locus_tag="ACG8ER_000383"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS5048996.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(48588..50048)
                     /gene="rhaB"
                     /locus_tag="ACG8ER_000384"
     CDS             complement(48588..50048)
                     /gene="rhaB"
                     /locus_tag="ACG8ER_000384"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS5048997.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            50201..51070
                     /locus_tag="ACG8ER_000385"
     CDS             50201..51070
                     /locus_tag="ACG8ER_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5048998.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(51196..52095)
                     /locus_tag="ACG8ER_000386"
     CDS             complement(51196..52095)
                     /locus_tag="ACG8ER_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048999.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(52260..53084)
                     /locus_tag="ACG8ER_000387"
     CDS             complement(52260..53084)
                     /locus_tag="ACG8ER_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5049000.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(53095..54390)
                     /locus_tag="ACG8ER_000388"
     CDS             complement(53095..54390)
                     /locus_tag="ACG8ER_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS5049001.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(54406..54885)
                     /locus_tag="ACG8ER_000389"
     CDS             complement(54406..54885)
                     /locus_tag="ACG8ER_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS5049002.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(54891..55919)
                     /locus_tag="ACG8ER_000390"
     CDS             complement(54891..55919)
                     /locus_tag="ACG8ER_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS5049003.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(55936..57042)
                     /locus_tag="ACG8ER_000391"
     CDS             complement(55936..57042)
                     /locus_tag="ACG8ER_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5049004.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(57271..59868)
                     /gene="adhE"
                     /locus_tag="ACG8ER_000392"
                     /gene_synonym="adhC"
     CDS             complement(57271..59868)
                     /gene="adhE"
                     /locus_tag="ACG8ER_000392"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS5049005.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        17740 a        12456 c        10520 g        19306 t
ORIGIN      
        1 tatcttttta aaaaactttt ctccataata aaaactaatc agaatcgtac aaatatttaa
       61 taaccaaata tatagaacat aacttaatat taagttattt gaaattccat aaaaaacgac
      121 tgcaggaatt attgtaagaa aaggataaaa atagttcagt ccatctccat aattgttaaa
      181 tgttgtatag ttgattggcc cttcaaaaat agaagataac cctttgattc gtgaaagatg
      241 aaaagataaa tcacctgttg tataaatttc ttgagcattt ctcataaaat ttactgaata
      301 taaagtacta agtaagataa gaactactaa aagaaatgct tttttactta ttttcataga
      361 tttgttgttc tcctttttta attctatttt cagtatacta gaattgtctt tttttaataa
      421 gttttgcgtt aaatttttca tagcactagt atgatatact agaaaagaat caatttttat
      481 tacttttaaa taggagaaaa aaatgaaaaa aaacaaaaac caaataatag atatcatttt
      541 tatgttattt ctaagttttg cttacgtcat ccctctattc atgtctaagg gaatctatca
      601 tagtgtcaat caggatactt actttcattt atctcgaatc ataggactag ataatgtatg
      661 gagttctcca gtaaacttca ataacttcga tcatcatgga acaatgatga atatttttta
      721 tccatggctt actctttatc cagctttttt attttataaa atgatgggaa atcttgtttt
      781 aagttataat atctactatt tttttatcac ttttttaaca atggtcgttt cttatttttc
      841 tatgaaacaa ataaaaaaca atcgttatat ttcgctgtta ttttcgatta tttatacatt
      901 ttccgcctat cgtgccattg acatattcca tcgtgcttca ttgggagaag ctgtcgcttt
      961 aacatttttg cctttgatac taatggggtg ttacgaaatt tatattagag attaccaaaa
     1021 atggtactgg ttgtctattg gcatgacgct agtagtgtat actcatttac tttctgtcgc
     1081 tatggtttct gtatttattg gaggaacgtt gtttctttct ttttattttt gggaccaaaa
     1141 aatcgctaga ctcttaagtc ttttaaaagc taccgtcctc accttttttt taagtgcggg
     1201 attcttaatt ccttttattc aacaatctag agcacaagaa ttgaaagtcc cactgggaaa
     1261 agaattaagt ggtatggctc ctagtgatat gctgacacat attttaaata ataactataa
     1321 caactatact attggattat tcttatttct cggattgatt ggtgctttta tttttataaa
     1381 aaaattaact gctgatgatt tatttatttt ctttcttggt gtatttgttc ttttttgttc
     1441 aaccaattta tttccttggc aactttttaa ccatacacca gttaagagtc tacaatttgt
     1501 ctggcggtta aatggttttt cttctttatt tatagcctat acgatgagta tagtgatcta
     1561 ttatattttt tctactaaaa ataattcatg gaaaataatg gtctttacgt ttcttgcact
     1621 aatattgcat ctctcaggag taagcaattc tatccatgct aacaaagatt cattaacact
     1681 tatagcacct gaagacgctg ttgctatagc tgaaaattac aatcatactg attatgcaaa
     1741 caaagagtca atctatcatc ctgatatgat taataatgat caatatttac taggtaacca
     1801 ggaaataaat ccaacaactc attttatgag taacaaactt acaattgaat tagataattc
     1861 gaataataaa aatactgtct tgacgacacc tatttatcgt tataaagggc aagtggcttc
     1921 tattaatggc aaactagtac aaacaaaatt atctaagttt ggcacaactg aattaactat
     1981 tcctccaggt ataaataaag tcgttattac ttatcagtac acaaaactag caattgcttc
     2041 aagatattta tctatcgtta ctttgatttt atttttactt tatcgattta ctttttctaa
     2101 acaaaaacaa ccgagaagag aagtacaaca ttctcactag atttttattt aagaaagact
     2161 ttttagctgt acacataaac aggctaaaaa gtcttttttt atatttcttt aatttgcttc
     2221 atacattatt taacatcgat atttatactg tgaaacatgc gtttcatcca ttatcaaagt
     2281 atttttatcg ataataaact tcatccaaaa acagaccttg tgcagggacg gtttctcctg
     2341 catgttgccg cactttgctt tcaaatattt catcgattgt ttctatcggc atggcacctg
     2401 tgccgatttc tagtaatgtc cccatgataa tccgcaccat tttatataag aatccgtctc
     2461 cgacaaaagt aaaatgaagc atttctccct ttcgttcgat agataattca tcaatcgtac
     2521 gcactgtgga tttttttgat tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca
     2581 ctaatttttc gcaagctgca ttcagcttgt ccatatccaa ctcttgaggg aaataaaagc
     2641 tgtggtttcg ctcaaatgct gaaggaatca cgttgttcca aacataatag ctgtattttt
     2701 tgcccaccgc attatatctt gaatggaagc gctcaggcat ttcttcgatt cttttgatga
     2761 caatatcccg cggaaggtaa cgattcataa agtcgatcat ttctgccagt ccaatttctg
     2821 tgtttgtttt aaaatttgct acttgtcctc gcgcatgggt gccagcatct gttcttcctg
     2881 atccaataat ttctatctct tcatttgtca tctgatgcaa tactttttcg atttttcctt
     2941 gaatggtttt atcgtcatct ccaagtcttt gccatcctga atatcttttt ccgtcatatt
     3001 cgattgtcaa cttgatgttt ctcatatttt agtggctcct ctgtcttcca tttggtcttc
     3061 tctctacctt tttaagattg tagcacaaaa tatagctcct tgcttctcta agacagaagc
     3121 tatacaagaa aactgtggaa atatatattt cagaaaaaca aaaaacagcc accggatgaa
     3181 gtatccgctg actgttcaat ttgacggctc aagttttact gtttgagatg caagatactc
     3241 aaccaggcaa tgggcacata attaaagaaa aaactgattc tttggcaaaa acctatttta
     3301 ttcagtaatg gccaagaatt tatagaagga attcttgcca gtccataaat cgccgcaaaa
     3361 agcaaactga tcacagacag tccaaggtat aagtggctta cgtccatttg cccatttttt
     3421 cgatagatca agaaaagaag aactggaaac aataaaaaac cactgtaacc taaacctgat
     3481 ccgatattat ggacccatgt cgagacattc cacgaagatt catgggtatt gatactgaac
     3541 aaacctgtaa agatacaatc acctatacca tagaacgcga tggctgctgt aactagggta
     3601 gctaacacac gagatgtact aacaaatgca gcatacaatg caggcaatga caggatgaaa
     3661 aagatccctg aaaccacaga ccaaataaga aacgcttttc ttacaggact ggaaacctct
     3721 ccaaatacac tgatcacgct cttcatttga tctaattcag gataatacaa tcctaatacg
     3781 tacggagtca aaaaatcact cagcacgcct aataataaaa agaaaaaacc aaatttacgt
     3841 aaaaaatcca tgatacgcct ccaataacaa ttacctatgc ttttaattgt tattataaac
     3901 gactttatct tgatttaaca gaaacctcta tttacacgaa catctatata actcattaaa
     3961 aactcgaatc attttaaaaa tactaaatat ctaacgttca acgttcactt aatccatcta
     4021 aacaatcttt caatttgatt ttttccccac ttcttttacg cgcgcctagc tctcctattg
     4081 cagtaaacaa cagatcactt gacgaattta aggcagtttc cattgaatcc tggataacac
     4141 cgataataaa tccaataccg acgatttgca tcgcgacatc attagagata ccaaacaaac
     4201 tacatgctaa tggaatcaat aacagtgaac cgcccgcaac acctgaagct ccacaagcag
     4261 ctaaagcaga caaaatacat aaaaggactt tcaaaacaaa aggtgcttca atccctaaag
     4321 aatgtacagc agtcaacgtc atcaaggaaa tggtaatcgc ggcaccgccc atattgatcg
     4381 tagcacctaa cgggatcgat atggaataag actcttttgt caatttcaat ctttcagcca
     4441 acatcatgtt tatcgggata tttgcggcag aacttcgggt aaagaaagcg ggaagtgcgc
     4501 tttctttcaa acaaaagaaa atcaatggat agggattttc tttaataaac aaaaacgtga
     4561 ggaatgggta cacaactaat gcgacaaaca gcatcgtacc gatcaaaaca agtaaaagct
     4621 gcagatactc tgccaatcca gcgattccag tcgtagcaat tgattgataa accaatccta
     4681 aaatccctat cggagcaaat gcaataatca cttgtacgac ttgtgacaac gctatagata
     4741 tctgatccac caactctttt gtcgattctg aacgtgctcg aaaagctagt cccaacaata
     4801 cagcccagaa taagacgcca aggtaattcg catcaataag agattgaaca ggattttgtg
     4861 tgacattcgt caacatagta gataaaacac tttccaaact ttgcggtgct ttttcagata
     4921 ctgtttcttg taaagtaata ggaacagtaa aaattgaact tacaataact gctacaagtg
     4981 cagaaagcaa tgttcccact aaatataaga tcaaaatagg cttcacgaat gtcttattcc
     5041 caattttatg cttggcaatt gacgccatcg tcaagaaaaa aaccagaata ggtgcgattg
     5101 ccttcaagca actgacaaac aacgttccca atattgaaat aaatgtccat gtggggaaaa
     5161 agataccaaa caatattccg actaaaatac caacaacaat acgttgaact aacgacatat
     5221 ccaaaaactt ctctaacaaa tgaccaccct ttcacaattt taacaatcat tgtatcattt
     5281 tatgcaaaga aactaaaggt ataattttct aaaaattcta acaatacaaa caaccatttc
     5341 ttgctaattt tgtacttttt ctgtctacac gttgtttttt gagatattct aagttaggat
     5401 gttattaatg ggcaacttat caggaaacgt tttcttatgt ttttgtccaa tttttttgtg
     5461 aggtgtttat tcttgaaaaa aataaagaag gagagttatg gcttcctgac aatctttgtc
     5521 gcttgtataa tgatggtttt acagcgtata ttgatttcag cagtcactat tcctacatat
     5581 gcaacaaacc ctggtcgatt ctacatctat acatttttac aaacttccgt tgttattggt
     5641 gcaaatttga tcccacttta catgggttac caatatcaaa aaatcgcccg tttaaaagtt
     5701 ttacattatt taagtcgttt tgcctttata ttcctaatag ccacactagt ttgcaatatc
     5761 tttttttacg tacaagctgg cagcttgaat attcgtgatt attggcttat cctaacacct
     5821 atcagtcaga attactttac ttatgctgtt tcttgtactt tgcttttttg tagtattcct
     5881 tatacagttg gatttttaaa taaattatcg aaaacaacaa ttaaatatct actattattt
     5941 ttaacaattg gattagttgg ctgctctact ctatttaata aagacccgtt tgcattacag
     6001 aatggacaga gtattttatg gattttttat ttatttttaa tgggttatgg tttgaaagaa
     6061 tggaactgga aagaaaaggt ccgctataag cttcctcaag tatttatcag cttgattgtt
     6121 ttattcggtt tgattatttt gatgacacaa atttcactca tcatcagagg aaatacagat
     6181 acagcgctga ggttttctaa accatattct ctcttcgcaa tgtattatac tttctcttcg
     6241 tttttatttt tagaaatgct atctgataaa ttaggtataa aaggaagagg aaattttctt
     6301 gctgtatttt tgattgtcac tcaagttata tgtaatttcc ctttagtttc ttatcgcgta
     6361 tctacattta tgaaacgtga atttccaaac tcagggaagg catggctgat aaatatcggg
     6421 cagtttttta tttgatattt attagcagtg tccctcctag ttatcttttt attttatata
     6481 caaaaaatcc gtttcattca tcgtttcatc gaatacttcg cttttgattc gattgaacaa
     6541 ttaactcagc gttggaaaaa aagaattcat tggctttttg ttcataaaag ttatttttta
     6601 gttgcagcat ttttttactg ctttaccttc gtgcagattt tcgttttaga accaaaagaa
     6661 ggttggaaag aaactatcca ggttgcctta aaaatattta ctcagcgaca agcacccatg
     6721 atcctgacaa tcatcattat tatgggattt ttcttcttac tattgttgat ttccaatcgt
     6781 ttttggtacg ccttgactgc taccttgatc attaacttac tgctcactat ttcgacagta
     6841 atcaaaatgg aaatgcggga agagcctgtc tttccttcag atttaaaaat gctgaccgga
     6901 ttgtcagaac tattatcgat ggttagccca gtcttattaa tcgtgggcgg attgatcctt
     6961 ctcctattat tgattactag tattattgta caacgtagac tacaaaaaca gtattccttg
     7021 aagattcatt ggaaacgtcg atttatcagt atcgctgtat tattaggatg tttttcgggt
     7081 gtctttttca ttaatcacaa aaattcgcct tctttcttgc tgtttaattt atttaaagta
     7141 aacaagactt tctttaacca gcaaggcgcc gtaaaagata acggaccagt cattcaattc
     7201 ctaaacaata tcgacattaa aatcatggaa gaacctgctg gctattcgaa agaaaaaatt
     7261 aatggaataa tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac aagaagtgac
     7321 tggttaaaca atcagacgat cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt
     7381 gtccctaatc tgacagtacc tacagaccct attccttaca tcaaaagcat tataacagaa
     7441 ggaacaggag gattgatgtt atccgtcggt tatggtggcg gaacagctaa catggaatgg
     7501 gaaggactga caggcttgag tatcagtaac ctgtctgctt cgttagttac accttatacc
     7561 cagctagtgg agcgtcagaa aataagtcca aatattaccg atctattcga tgaaaaaata
     7621 gccatccatc cttttacagc tgctctttat cgcagaaaac aagtgtttga taaatttggt
     7681 tttgacaggt tctattattt agacagtccg gataaattaa cttatacgga taaaatagaa
     7741 caaagcccac gcatatcgga tgattcagca tacaaagaaa ccttgaaagc tttacaaagc
     7801 aatacggaaa cgtcacaatt catccaatta tccactatgc aaaaccatat gccttatgat
     7861 gattactata gtgaattgaa ctacactgct gaaggaactg ctgtcattcc taatagaaga
     7921 aaagaattac aaacttatat gcaaggcctt cactataccg atacagcagt aaaaaaattc
     7981 atccaagaaa tcgacaaaat cgataaaccg attacctttg ttttttatgg tgaccattta
     8041 cccgcaatct atagtgggaa tgattcgaaa aaatatggat tagaacagca ccagacagat
     8101 tatttcattt ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt
     8161 gttgctccta ataactttgg tgctatggct cttgagcatg cgaatatcaa agtaactcct
     8221 tactatgcgt tgttgacaga agtatctaat catctacctg cattaacaat tgatcctaca
     8281 gaaagcttgt ctaatcgttt taatggaaaa caggtctttg ttacagagga tagtcaaatt
     8341 atccaagaaa aaaatttaac tgagaaacaa aaagaaattc tagaagatta tcgtctgata
     8401 caatatgatc ttgttgcagg taaccagtac agtgcaacat gggcgacaca aaaaataaaa
     8461 gactaacaaa taaactgagc aaggggttgt gataaaagac atgtcacaac cccttgttta
     8521 tctcattaat ttgcttgtcc ataaacgatt tgattaaagc catttttcaa ttcattattc
     8581 gctgtctcgt tgaagtaaag ataattatcg ggattcccct gattttgttg tgtcaccaat
     8641 acttttggct gattttgctg aaacccgaat agatagacgt gctttttcag atatggttct
     8701 gtctctgcat cggaatagac ggctgtgatt tgatagtctg cttgacctgt cccactttct
     8761 gaccattctg cagaaacagg cactccacca atagccatct tccactcccc gttcaaaata
     8821 gcttgaggga ctttaagacc atataagtcc acactcattt gattcgtata acttttgtat
     8881 tgttgtccca ttgtttggcc ccatttggtc ataaatgcct ctaattgtgc ggatttgtct
     8941 gcattccaca gtgtttgttc aacttgttcg ctgctttctg aagaggaaga tgaagcctct
     9001 acagaagaac ttgaagtaga tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa
     9061 gtagaggtat ctgctgtttc tgtacttgct gcagttgact tgctctctgt gctagttgtt
     9121 gtagtagttg caactgttgc tttcgctgtt tcttttgttg attctttttt cgattcattc
     9181 ccgttggtac aaccagctaa caaaaaagct agaccaattg ttactaaaat cctttttttc
     9241 aattaaaaaa ctcctttcga ttactcccac ttctttattc tatcaaataa gcaatgtgat
     9301 tttattgatt tttcgtattt tctcatcaat ctttcaaatc ttctaaaaac tgagaagcac
     9361 ccgctgatgt tgtctgtaat acatcagcgg gtgcttcttt tattttaatc ttcgtgattt
     9421 tccaatttac ctcacgcttt atatttgtat ttgaagaaac atagactttt agtcatcatc
     9481 gtcttcctca tccaaatctt gtcggacagt ttttccttgg agtctttgta cttggtagat
     9541 gatttcttta ttgaaactta aaagttcaac aagcaattgt tgcaagccat atttttcttc
     9601 tttttcagct aatttgattg cccgtgtgat aaagacattt tggaaatcaa aatcttttgc
     9661 aaagttttcc aacattgttg aggctggtaa atatttgttt tgaccatgtt cgtaaatttt
     9721 tccataacgc atgacttctt ctaaagtaga cggaacgatt tcattttcaa tcactaactc
     9781 atgagagatt ttattgaacc atctcgcttc ttgatcagca tattctgaaa aaagggtact
     9841 taattcttgc gaagatgtac ccaaaacata caattgtgct tggatcaatt tcatttcttc
     9901 taatttaaaa tttgctaata catgccccac catggcgcca gcagttggtt tatgatggtc
     9961 gatatctgct tgttttgttt cttgaacaaa tttttcttcc actatattca ttgatttacg
    10021 cctcttttac ttttgctgat tggacttcat aacctaattt ttccacaact gcagctaatt
    10081 gttcagggtg gacttcatct ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa
    10141 ctttcgtatt gcttacccca ggctgttgag aaaccgcatg ttcgattttt tgcatacagc
    10201 ttggacaagc tagtgtatct aattgcataa taattttttt catcgtagtt cttccttcct
    10261 ttttcttgat agtcttattc tagattacag cttccataaa aaaattgatt cttatcaagt
    10321 tttacgattt ttcttctttg ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga
    10381 taacgatcaa gatactagct tcgtgaacga acatcccact agccatgtaa ataaacccgg
    10441 caaataagcc gttgagtaaa aataagacga caccaatggc aatcactaca ttttcacggg
    10501 tatttaaaac agttttctta gccaagcggt aggcatgaac caacgattca aaattagatt
    10561 gcatcaagac aacgtcactc gtttcgatag caacatccgt tcctgatccc atagcaatcc
    10621 cgatatcagc cgcagccaaa gagggagaat cattgattcc atctccaata aaagcaacgc
    10681 gcaatccttg ttcttggtat tttttcacaa attgaacctt ttcgtctggt aataattcag
    10741 catggacttc atcaatgcct aacatatcag ctacataatc agcagtcatc tgattatcac
    10801 ctgtcagcat cactaactgt tttgctcctg cttgtttcaa cttagctaat tgttcagcga
    10861 cttctggtcg aatcacatct gacacaccta tgatttgagt gatttttttg tcgattgcta
    10921 caataatgac cgagctgcct gtttgttgta attgatgtaa atcttgtact tgctttggtg
    10981 tgagagaaag tttgtgagcg gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat
    11041 caatctcagc tgtgattcct tgtcccttga ttgtctgatt atctttgact gctaattgtt
    11101 gaaaatcaat gtctttctcc tgagcataat ggatcacggc gcgccccaga ggatgatcgg
    11161 ataacgtttc taatcgagcc actaaagcta agctgtcccc ttcatcctct gtatagttct
    11221 tgatagtgga tacagcagta gttcctttgg tcaatgtacc agttttatca aagacaaaag
    11281 tatcgatttt ggaaaaagta tccattgctt caccgccttt gactaagatg ccccgtttcg
    11341 ctccattacc gattcctgct acattcgaaa caggcgcacc aatgactaaa gcgccaggac
    11401 aaccaagaac caaaacagtg attgctaaac gaaaatcacg actaatcaga ccaacaacca
    11461 acgcaataag taaaacggtt ggagtataat actgagaaaa tcgatcaatg aatttttccg
    11521 catgggattt ggtatcttgt gcttcttcta ctaattcgat aattttggca aaggtcgtat
    11581 catctccgac cttagttgct tcaatcgtca cgtagccatc tgctaaaatc gttccagaga
    11641 agacttcatc tccactgatt ttgacgactt gtcgtgattc accagtcact gcggcttcat
    11701 ccgcataccc tttcccgtca atgactcgtc cgtccacagg aatagctcca ccagttttta
    11761 ctaagacgat atcgccttct tccacgtcat cgatttccac ttcttcagtc gttccatctt
    11821 cttggacaac actagcagta gatggggcca tctcggtcaa agccttgatt gactcccgcg
    11881 ttttggtcaa tgttttttgt tccagatagc taccaaacaa aaacaagaat gtaacgatag
    11941 ctgactcctt gtattctccg atcataaagg caccaagcac agcaatagta acgagtaaat
    12001 caattgagat aactttgact ttcactgcct gatacgcatg gatcataata ggtatgaccg
    12061 caataataga agctatggct agagcaatgt tatagaacaa gacaaaacca gtaaattttc
    12121 cgataaaacc aaaaataatc aacacagctg ataaggcggt gatatgattt cgatgctctt
    12181 ggatccactt ttgtaatttc atctttctcc ctcttttcct ttcattctta cgagactaag
    12241 tatagatact tatccatcat aaaaaattga ccactatcaa gtttagtcaa aaaggcggtt
    12301 ttcttttggt agaatacact acaaaaaata gttagcaaga agactcacta aaagtgatct
    12361 tgccaactat tgtcgtttct atcgaaattt tcattgaatg attttcctgt acagcataca
    12421 tcggagatct atttctttat ctaagatttt tatcttgcta attttttcct ttagtctttc
    12481 aaatccacgt cgttcataga attgatacgt acagccacta tctgtaaaaa ggaatatttc
    12541 ttttcctttc tctctttttt caaattctgt tagcaacttg gtaccgatgc cttttgtttt
    12601 caaatcagga tttgcagcta aaaaaatgat ctctccatct ggcgaatgct tctttaaata
    12661 tgctgcaagc atttgttcat ttgctgtctc gtaagctcct ttactttctt tagcaaatat
    12721 attttgtaaa aaatcgaata tcttaacata tatttgctgt ccaaaagaac gatgtttctt
    12781 tttttctcct tttatctccg ccagcaaaac acctgccaac tcgtcctctg catacaaagc
    12841 aagaatttga gttgctcgat tcaactccat atacaaaaaa tatcttccgt aaagattcag
    12901 cagaagttca ttttccatat atctctcaaa gttcattcct tcaacagcaa aatagatagc
    12961 ttttttgtaa tctttctttt ctaagggttt aaaagttagt ttcatcattt cgcctcctca
    13021 acttcaatga ttgtgttata cttacattat aatagacaca gtgtctacta tctataatca
    13081 atcatctata agtttgtcga ttaagcgaca gctttgcttt gatatgtcgg ataaggagat
    13141 aggtaaatga aaaagacaga tagacgcgtc aaaaaaacag aaaaagcttt ggctgaaaca
    13201 ctatccacat tattagtaaa taaaaagatt caagcaatca ctatacgcga actaacagaa
    13261 acggcagatg tacatcgttc gactttttat acacattata aggatatcta tgatctatat
    13321 gatcaattag aaagcaactt ttttcgggat ctaaatgaaa ttctctctta tgatccagcc
    13381 catagctatg aggaactata cactcgcttg attgactatg tttacgcaaa tactttttta
    13441 tatcgcttat ttatcctcaa ccatggtgat atttcgtttc agaagaaagt taaacaagtt
    13501 attgaagaaa actatctgaa catatggcta tatgaagaca agaaaacgat tatcacagaa
    13561 gaaatgagat tctttactac gtaccacgtc caaggttgtc tatccgttct caatcggtgg
    13621 gtagaaagca attacaagta ctccaaaaaa gaattattag aactcttaca gaaactgaat
    13681 gatcacatag agaaaatcat gccttgaata aaaacatact aagcttagtt aagaagaacc
    13741 gtaaccttct cgtataacaa ctgaatctta tttagccaaa ttgtttactg aagatttttt
    13801 gagtaaaatt tctataattc ctacttattg aaaagtagtt tgtagtgctg cttctccctc
    13861 tctaagtgat atatcttgtt gtaccaatca aattcacctc atcattaata aattactttt
    13921 aggctaattt attacaactt ataggttgaa gctgtaacca atttatagtc atattatatt
    13981 tttatcatcc agtttattga attcttctcg tttacctcaa ttcattttca aggggaatca
    14041 gctcatcatc taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt
    14101 tcgcttctgg atgtttggct ttatatcctt cgtaatcctt actgttgata attgctttca
    14161 tattaggaat tggatcttca cctggcatat attgcagatt agaagcatgt aattttttct
    14221 cagaaacaaa ccactcattg gaaaagacat gaatcaaatc atcacttact tgtgtaaaat
    14281 agcgacgttt aatgacaaag atatcttcat tgctatctag tgtaccttca tttctttgtg
    14341 cttaaattca aagataattt tctcaataaa gatttttgat tccagtaaga ttcctttact
    14401 ccttttacaa catagcgttg tcctttgttt tgaaacatct tcttcccctc tttgccatta
    14461 aaaatagccc aattctctat aaagaagatt aagcttcatt attataatat aaagtttaaa
    14521 tttggtttat tacttacttt ttcttattct ctagtaagct aatattgaaa aaaatgtaat
    14581 cagatcatta tttttatatg ttgtaacttc ctgataagaa aactaattaa aagaaagagg
    14641 taactacata aactaattaa aaaagctaac ttcatagttg gaggataaat ggaagaaact
    14701 tcaatgattg tttctccttg tgttccttca atcgacacta gtcctattat caatctctct
    14761 tttttgtcca tttacttttg cgactgttcc ccaataatca aatacaggaa gcgttatttt
    14821 ttgtccttta gattcatgta ctgtatagac tattttcgaa tccatcactt gataaggcat
    14881 agtatgccat tccttgccaa ttcttacttc atgattctgc aaatgttctt tgtattgtaa
    14941 tgcttccgtg ggtgcatagt cataaagtcc tccgggaagg agtgtcgtgt aacgaataag
    15001 attttcttga ttcattacca agtgatctct ctgagtgaca actttatgtt catagtttgc
    15061 ttttgttgca gcagatacag ccaacagtaa taaaacaatt gccgttatat taactacttt
    15121 tctttgctta gctgatgtgc tcttcatgaa aacgaacgat aaaatcaacg agccaaacag
    15181 tacttgaaag cctaagattc gccaaggaaa ttgaatcatt tgaatgggtg tattttgtaa
    15241 taaagaccat ggaaaaaatt tggtggataa gagccaagag actaccgcac taaggtaaat
    15301 cactttttct ttgcgattaa atcctttaaa aaagaagagt ggacagatca aagaaataat
    15361 accaatgatt cctaccgtaa aggttgtcag gtcattattc atcgcaccaa tcaaactatc
    15421 cgagacattt aatgcttggt gttgtaaggt cgttttatct ggctcattaa tcgattgaac
    15481 catgatttgt cggaacatcg gataccaaaa gtatgctgtt aaaagaatcg tcattcctac
    15541 tgcctttgac aactccacaa tcaaccggat gctttctttt tttcttgttt cacttactac
    15601 aaagccgcct aagactagta agccaacggc tattacactc ataaaagtgg aaagaacatg
    15661 tgtataaatg agtaagctca tactgactgc taatggaatt gcacatggct ttttctcata
    15721 aatcagtgcg taagcataat acataactat cggtataacc gtaagtgcaa tcgcttcagc
    15781 aatagcagaa cgatgataaa tatcaatcgt acggtaccca taaaatgtat ataaacagct
    15841 aaatataaaa gccgcatcga ttctttttaa aaattttagt ccataatgaa acatcagcat
    15901 gatagtacaa acattcaata gccaaacata taagatataa ctaacgatta aattattact
    15961 tatccaataa aaaatgactg ccggaaaaaa ggtcaagtag ggataaaaat aattcactcc
    16021 gctcccataa tgattaaacg tcgtaaaatt gattggacca gaaaaaattg aagataaccc
    16081 tttgactcta gcaaaatgga aggcaatatc ccccgatgta gaaatagtat gaacatttct
    16141 aaaataattg attgaataaa gtccagcaat aattgctagg agaatggaca atattatttt
    16201 tttctttact tgcattactt aattatatac tcctttcatg gattgcttta tctagtataa
    16261 cttaaatttt aaataaatcg ttatttattt ttactctcac ttaaaaaagc actaaaatta
    16321 taagagaggg aggtaatggt ctgaacacca agaattgttc caacatcgaa ttgcttaact
    16381 aatgagttca aagcttaatt atgtggctct tatatttacc gagcgtggat tgcgatctct
    16441 ttagcgaaac aaacatcttt cataaaaaaa acagccatat ccgctttctt ggatatgact
    16501 atttactact actttactat ctttcattct tcttgtctca cttatgataa ggcgcccccg
    16561 cattgatccg gaaggcacgg tagatttgct cgacaaggat cagacgcatc aattgatgtg
    16621 gatacgtcat ttttccaaaa gagatctgag cattgcttct tttcatgacg gcttcgctga
    16681 gtcctaaaga gccgccgata acaaaaacaa ggtggctttt tccttggata cctaattggt
    16741 cgatttgttt agcaaaagat tcacttgtag gatttttccc ttcaattgct aaggcataga
    16801 cgtattcttg ttctttgatt ttcgctaaga tccgttcccc ttctttttct tttacttgtc
    16861 tcatttgcgc ttcgcttaaa ttctccgggg ctttttcgtc aggaacttcg atcaattcga
    16921 tttttgcata ggcattcagc cgtttgacat actcattgat accttggatc aggtattttt
    16981 cttttaattt tcctactgag ataattttga tattcatgtt ttctctcctt tggtcttcat
    17041 gctcactttg tttattataa tcaatttttt cgtctatatg tttattttgg ctatttttgt
    17101 taaaaagatt tccccaacag ttatgcacaa catccacagg tttatccaca gaaaacagtg
    17161 ttattttacc ttatttcatc acattcatta ggcaaacata cgttttataa gaatttatcc
    17221 actttataca caggctgtgg atatgtttta tcccttattt ttccaatcct atccgcgtag
    17281 ttttacacag tatctgtgga aaaagaaaat caaatatttt catctgaaaa caatgatttt
    17341 tcctaaagtt tttcataaaa agttcacagt ctcaaagtag actaaagtca acaaataaga
    17401 ctatactaac agtagataaa tgaatgtaat tggaggagga acacatggat agaaaaaatg
    17461 tgacaccaaa gatgaaaaaa agtaaaaata atagcctctg gcgtaagtta ggtctggggc
    17521 ttgttggagg gatcgttgga ggactactga cagctggcat tttttatgca gcgatgggca
    17581 ctggcaacaa tagttctact gctacaagcg gcaatcagaa ttctgccggt gaaacagttg
    17641 tggaaaatgt caaagtcaat gtagattctg atatcaccaa agcggtagat aaagttcaag
    17701 acgctgtcgt ttctgttatc aacttacaaa gtcaaaacca atcaagcggt tttggcggac
    17761 tattcgggca gcaagaagaa agtagcagct cagatgatag taatttggaa gcttatagtg
    17821 aaggaagcgg cgtgatctac aaaaaagacg ggaatactgc ttatgtagtc acgaataatc
    17881 acgtagtaga tggccagcaa ggtcttgaag tcttgatgaa agacggtacg aaggtaaaag
    17941 ctgaattagt tggaacagat gcttattcag atttagcggt gttgaagatc aattcagaca
    18001 aagtcgaaac agttgcctct tttggtgact caagtgctct gaaggttggt gagcctgcga
    18061 ttgcgattgg ttccccacta ggatctgaat atgcaaattc tgtcacttca ggaatcattt
    18121 cttccttgaa ccgacaagtg acaagtacga atgaatcgaa cgaaacagtc aatatcaatg
    18181 cgatccaaac agatgcagca atcaaccctg gaaattctgg tggtccttta gttaatatcg
    18241 aaggacaagt tatcggtatc aactcaagta agatcgcaag tacttctgaa tctacttcta
    18301 atgtaagcgt agaaggaatg ggatttgcta ttccaagtaa cgatgtagtg aatatcatca
    18361 atcaactgga aaaagacgga aaagtgacac gtccagctct aggtatcaca atggtcgact
    18421 tatctgccgt ttctactcag caacaagaac aaatcttgaa gattccttca tctgtaacta
    18481 acggtgtcat cgtgacaaat gtccaaacag ctacaccggc tgaaaaagct ggattgaaac
    18541 aatacgacgt cattactaag atcgatgaca aagaagtttc ttctggtgta gaacttcaat
    18601 ctgctttgta taagaaaaaa gtgggagatt ctgtcaaaat cacttactat gaaggctcga
    18661 aagaaaaaac aactactgtt gaattgacga tcgatcaatc ggcattaaaa caaaatcaaa
    18721 atagtgaaaa ttaataaaaa agaggttgtg acaaagactt gtcacaacct ctttattttg
    18781 gaaatgcttc tccgactacg gcatcctcag gcaatactaa gattcctttt tttgctggag
    18841 catttggcaa atgcaattca cgagcggaac agatcattcc atagctatct acccctcgca
    18901 aagttcctgg ccagatcatc attccatctg gcatcatagc tcctggtttt gcaacgacaa
    18961 ctttttgtcc agcttcgata ttgggtgcac cgcaaacgat ttgcaatact gtaccattgt
    19021 ctacttctgt ctcagtaatc gataaatggt cagaatcagg atgtgggacg catgttttta
    19081 cgtacccgac aacgatcttc ggttcatgat cagcttcaag ctgttcttcg aatcctgctt
    19141 ctttgatcaa tcgattcaat ttcgctaatt cctcatctgt taagcttact tggccgttcc
    19201 cttcgattgt tagatgatcg gagatatgga aaaagttcca agcaacaact tgaccttgtt
    19261 catctttgat ccgagcaaca ttccatttgc gttcaacagt gttttctgtt cctttatcgt
    19321 cggctataat aaccatcaag gtgtctccga cgtgtgcttt gttataagca aaaatcatgt
    19381 tctttcctcc attatccttt gctgagattt atcttaacaa aaaattgcat tcactgcatg
    19441 aaattttttt catgctttat ttccgaagaa gcctcttctg gaacaccatt tattcgtaac
    19501 aagggactgt gacaagactt ttgtcacagt ccctttactg ttttagttaa ttgattaaaa
    19561 aggtcgtaca agttccactt gcttaattac ttggctgttc ttcttcgtca gaaacgatca
    19621 agacatcaca aggtgaatga cgtatcacgt agctggctac acttcctgtc ataaagcgtt
    19681 cgactgcatt tagacctgac tgcccaacca tgattagatc gatctcatat ttttttggca
    19741 atttgactgc catggattca cgaacagaac catatgcaat aattccttct atatttgtgt
    19801 aatcaacaga tttcccatat tctttacatt cttcgatcat ttccttggct gtttcagttt
    19861 gctgatcgac tagattttga tcaataggtg catagcccat aaagttataa aattgctgat
    19921 cgattacgtt tgctacatat actttcccat tattacggcg agccacttct accgcttttt
    19981 tgaaagcagc aagtgcttgt tcactaccgt cgatacctac taaaatattc ttatattctt
    20041 gattcatctt gacccctcct aacagatgtc agccttttgt tacactttct aggaacgatt
    20101 cgatttcctg ttttgtttta cgatctttat tcactagacg acctaattct tttccgtctt
    20161 ggatcacgac aaaacttggg ataccaaaaa tgttccattc agcggctaca tctatgtact
    20221 tgtcacggtc tacttcaata aattgccaat cagaaaagtc attttcaatt tcaggcattt
    20281 gcggtttgat aaaacggcag tctccgcacc agccggcagt aaagaaaaat acactctttc
    20341 cagtttctac gtagcctgct aattcttcta aactatttgg gataatcatt ctttgtcctc
    20401 tactttctgt ttatgtattt ttcaattaaa ttatacacct ttctttcgaa ctaacaaaca
    20461 ttgatcctct ttttataaaa aaaatctggc aaaaagcaat cttttgccag attttatagt
    20521 ttcactatcg cattattttc cgtatacgat cgtatttact gttgatttat ccaaaccttt
    20581 cagtgtttga atcaacattt ctcgtgcagc ttcaaaatcg cgaatactga acatcgtttg
    20641 atgcgtatgg atataacgtc ccactacgcc aatcactgta cttgggattc cttcgttttg
    20701 tgtatgagca gcacctgcat ctgtaccgcc ttttgaaaca aagtattgat aagggatatt
    20761 gtttgtttca gcgatatcca gcaagtactc gcgcaaacga ggaagcatga tcaaacctgg
    20821 atcttggata cgcatcaatg taccttctcc taaatgtccg aatgtgccat ttttcgtcac
    20881 tgtatcatct gctgcagagc agtctactgc aaagaacaga tctggcttga atttattgac
    20941 agaagcttta gaaccacgca agcctacttc ttcctggaca ttcgcgcctg caatcaatgt
    21001 atgacctaat gtttcatttt gcaacgcttc taaagcttct aataccattg tacagccata
    21061 gcggttgtcc catgatttac tgataatatt ctttccgttg gctgttttga tcgtttcagc
    21121 gtaaggaacg attgtgtctc ctggaaggac accgtaactc attgcctctt ctcttgattc
    21181 aaacccagca tcgaacaaaa tatcggttac ttctactgat ttttgtccgc ttgttcctcg
    21241 caataaatgt gggggaacgg aagaagaaat acatggataa ttcttaccag tagaggtttt
    21301 aagtgtaaaa cgctgagcag aaacaacgta aggattccag ccgcctagag gtacgacttt
    21361 aaaaagcccg ttttcttgga tttgtgtcag catgaatccg acttcatcca tatgtgctgc
    21421 gaccattaca cggggtgcat ccgcttcttc atgatggcgc agaccaaaaa tgccgcccaa
    21481 accatctaac tgtaactcat ctaccagcgg ttccatatga ctttccatat aagcgcgaac
    21541 atcttgttca aaaccgcttg ttccttgtaa ttctgttaac tctttgattc gttgaaatgt
    21601 tttttcttcc atatgctatt cgtctaaatt tcagactaaa ctcctctcag acttatattg
    21661 atctgcggat aaaatatgat tttttctgca gaccgatcgt atttattata gcttattttt
    21721 tctggtagcg gttatttatt tcaaagaaaa tatgagagtg tggtaaaata agcacaagga
    21781 gtaacaagtg agaagaagga ggtatgtcca catgtatgaa gaaagcgaac taagctattt
    21841 taaaggcggg ttagcagttg gaatcggtct aggattattg agcggggtag cttcaacgat
    21901 ttggtatcat cgcaaaaaag tattgtcagc agaccaagta cttgaaaata tcaagtcatc
    21961 ttttctaaaa gaaggtgcaa tcgaaggttc ttggatctct tttgaaaagg aacctattag
    22021 gaaattcgcc gttcggtcga gtgcttatcg gggaggtctt tctcgaatag aagatggcga
    22081 aactgtttat tatgaatttt tagcagatgc tcatacagga accgtattag aaatttcaag
    22141 aaaaaaaggt gcaacagatt aatacgtcaa aaaagtgaga gaacgaatac gcacccacta
    22201 ttcaaaagag aggtatcatg tccttctttt gtagtgggtg gttttttaga tagagctttc
    22261 caaacatata ttccagtatt ttctttttaa ataaatgata tttagcggga aaaactagca
    22321 tttcctttga ttatgaggta tttttaattt agcttttgtt ttctatagtt aaggagagtg
    22381 aaaaatggaa caacaagaaa aaattgctgt tctgattcct tgttacaatg aggaagcaac
    22441 aatttcaaca gtcattgcag actttaaacg agaattacca gaagcagata tttatgtcta
    22501 tgataataat tcgaccgatc gaacatacga attggccgtt caaggtggag caatcgtaaa
    22561 aaaagaacct cgtcaaggaa aaggaaatgt gatccgccaa atgttctttg atatcgatgc
    22621 cgactattat ttaatggtag atggcgatga tacttatcct gcagaagctg tacatggttt
    22681 gctcgagaaa ttacgttctg gtgaagcaga tatggtcatt ggtgatcgcc tatcaaatgg
    22741 aacctacttt gatgagaata agagaccttt ccacgatttt ggaaataacc ttgttagaaa
    22801 tacaattact agaatgtata aaacaaaaat tcttgatgtc atgaccggct atcgaggatt
    22861 caatcgtatc tttgtcaaaa gttttcctat tatgagttct ggttttcaaa tcgaaacgga
    22921 attgacgatc catgctttag ataaaaaatt caagttcgtt gaaatcccaa tcgattatcg
    22981 tgatcgtcct gaaggcagcg aatcaaaatt gaatacattc tctgacggat tcaaagtgat
    23041 catgatgatt attaaaatgt ttaaagatta taagcctttg cttttctttg ggatttggac
    23101 cattttcttc tttttatttg gattggtcac ggggattcct gtgattcgcg aatatatgct
    23161 gacaagcttt attacacgca tcccttctgc gattttaagt actggattga tgactttagc
    23221 acttctttca ttagtaacag ggttgatttt ggatacagtc gtcacgaacg ctaagaaaga
    23281 atatgaattg aatctatatc atgtttatca tgaataccac agtagaaagt aaaaaaagga
    23341 tcaagactgc aaaaataatt tgcaattctt gatcctcttt ttaattaatt atttcgccaa
    23401 acgatagatg gcatctgcat agattgctgt tgctctgaac aagtcatcaa gactcataaa
    23461 ttcgtttgct tgatgcattg tatcagtata tcctgggaac atcgctccat aagcaacgcc
    23521 acgtttcaac aaacgtccgt aagttccgcc accgatgatt tgttcgtagc ctttttctcc
    23581 agtgtggtct tcatagactt gcaataatgt agccaccaat ggatcatcca ttggtacgta
    23641 atgtggttcc attacgcgag cacctcgagt aactgttgcg ccttcttgcc ccactgtttg
    23701 ttcgatgtca ctttgcaatc cgtctactgt tacacctttt ggaaaacgga aattcatatt
    23761 gatgaagttc gcttcctctg taccattttc ttcaaaagca aatagtccag cattcattgt
    23821 caatttgccc attttttcat cttcataagc gactcctagt ttttctccat aaaaatcttc
    23881 atggacataa gcagctgcta catggataaa ttgtttagca gatcctagga attcatagtt
    23941 atctaagaac aatgccaaga aagaacctgc attgatacca gattgtgggc ttgcaccatg
    24001 tgcgccttta ccaactaatt cgatttttac atacgtattg tccgcttcaa tcgttccgct
    24061 tactggattt gcttcgatga attggtaaaa tgcttcttcc atcgcgatag ctgcatctgc
    24121 acttggtact tgaagtgctg cagttgctgt tcctggcacc atattttcgc gaagtccgga
    24181 aacaaaagat ttcaagacat aatctcctgc attgtcgcct ttgaagcgta aagctaaaga
    24241 aacatttcct ttttcaccat tgatgattgg gaattcagca tctggtgaga aaccaaaatc
    24301 tggttcttct tcatgttcga aataataagc catatcgccc cagccgcttt cttcatcgct
    24361 gccgactacg aagcgaactt tttttgatag tttcaagtca agttctttaa tgatcttcat
    24421 cgcatagtaa gctgccatac ttggaccttt gtcatcgctt gatccgcgtg caaagatctt
    24481 tccatccttg attactggtt cataaggatc tgtgtcccag ccatctcctg caggaacgac
    24541 atccatgtgt ccaaatatcc ctagcgtttc gtctccttcg ccaagatcaa tatgccctgc
    24601 atagttatcg acatttttta ctttaaatcc atcacgttcg ccataagcta acatatgttt
    24661 caatgcgtcg cgaggtccag gtccaaatgg tgcatctggt gttacttttg agtcatcgcg
    24721 ctcgctgttg acacgtaaaa gatttttcaa gtcttcaagc aagtcttctt tacgtgcttc
    24781 tacttctttt tgccaatcaa ttgtcataaa gttaatcctc cacttattta agtcttcgtg
    24841 ttcatgatac cacaaattac agaaaaaaat agttcaaaaa atctgacaat tccttttctt
    24901 atctgttttt ctctaaaaaa aatatacgct ataatgagta aaaggaggtt tttagatgct
    24961 gataaacgaa caactagtga aaagagtcat cacacctcgt ccaaaaattt cacataaagg
    25021 aacatttgga cgtatcgttt tgattggcgg caatgaacaa tatggcggag caatcattat
    25081 gagtgcttta gctgccgtac acagcggagc gggtctcacg actgtagtaa ctgatgaaaa
    25141 aaatcggagt gctcttcaca gtcatctgcc agaagcaatg attgtagttt ggacggatac
    25201 tgaccagttt ctttccgtaa taaagcaagc agatgtactc ttgattggtc ccggattggg
    25261 tacttcttct aagagtttgg aacgtttcaa acaagtacta gaaaaccaat caaaagaaca
    25321 atggctggtg attgatggct ctgctttaag tttacttgct gctaatcatc ttttgctacc
    25381 ctatcccgaa caaactgtac ttacacctca tcaaatggag tggcaacggt tgagttctct
    25441 accgatacaa gaacaacggg ataaagaaaa caaaaagaag caacaggaaa tcggtgctgt
    25501 gctcgttttg aaaagtcatg agacaaaaat ttatggtcac cagcacttct acaccaatcc
    25561 tttaggttct gctgctatgg caacgggcgg catgggtgac actttagctg ggatgatcac
    25621 aggattttta gcccagtttc ctcaaaatga ccaaacgatt gcagcagcta tttatcttca
    25681 tagtttcatt ggagaccagc tggcaaaaga acaatatatt gttttgccga cacaaatcag
    25741 cgaagcactt ccgaaatgga tgaattattt ttgtaaaaaa tcaatggaaa acgaggcatg
    25801 agtcatggag aaatttacag gttatgtgcg agaacctttt tattatgaaa cagatcaaat
    25861 ggggattatt catcacagca attatatccg ctggtttgaa gaggctcggg tagccttatt
    25921 ggtacatcta gggtttccct ataaaaaaat cgaagaatct gggatcatca tccccgttct
    25981 ggaagtaaat tgcagctata aagaaatgat ccattacgga gaaaaagtga gcatccatcc
    26041 aactatccaa aagtatacgg ggacacggtt ggattttagc tacgaaattg tgggaactga
    26101 agacaaaaaa ttaaaaacta ctggcagtag taaacactgt tttttacttt ctgattcgca
    26161 acgattagtg aagctgagca aagtcaatcc ggagttagat cagttattcc aagcgtacgc
    26221 ttctttggat gatgtaaatt cttaataaag accgtctaag catttttctt gtattgtgtc
    26281 gagtagttcg tataatgaaa agaaaaaagc aggtgatccc catgtatgtc gttaaagtaa
    26341 tgcacggtta catcgataaa actggctgtc gcactcggga aaaaaactta gacaatctgc
    26401 tcattttcaa agacaaaaaa gaatccgaag cttttgctaa aagaatcggc ggtcgtgtca
    26461 aaccgatcca agaggtacga ccagattgat tcaatatttt tcttttcgag gctctaatag
    26521 gcatgcctat cagggctttt tttgcagact gtgtttttat gcccacacga tttgtaagcg
    26581 atttatgtta taatagatta tatctgcata gaaaggaagg ttaaatggtt atcaataatt
    26641 tgcaacagca agcttatgaa tctatacgta aacaaatcat ttattccgag ttagcacctg
    26701 gaagtaaaat ctccgataaa gtgttagaag aacaactagc tatcgggcgt acaccgattc
    26761 gagaagcact gatccaactg cgcaatcaag agcttgtaaa aaccattcca cagtccggca
    26821 cttatgtttc aaaaatcgat atccgatctg cttcattagc ccgttatacg cgggaaaagt
    26881 tggaaaaacc gatcctgcaa gaatgcagtg cgaaaatgac caagcaagat caagctaaac
    26941 tagaaaaaat actagaacaa accgatcagg cagtaatcgc tcaaaataaa aaagcttttt
    27001 tgatttggat actgattttc accgaacttg ttatgaaatt gctggaaaac gagaaatttg
    27061 ggattggcta gaaagttata gtacgcactt gaaccgtttc agatggttac gtttgacgat
    27121 ttcagaatta gactggggtc gagtattaga tgaacatcag acaatgcttc aatcaatgat
    27181 tgatcataac tttgatgaag tcggatttct ttgcacgatg catttacata tgattatcga
    27241 ggagcaagaa tacgtgattc ataattaccc cgattatttt gaagatattc aggacagacc
    27301 catcaaataa gaggttttgg aatcagcgtt ttgacctgag tattagagaa agaaacggca
    27361 aaatagttcc tcatattttg ccgttttttt tgagctaata ccgagggtca gctgattgca
    27421 caccgtttat tgagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag
    27481 taaaaatagc ttcttatgtt tttaacttgg ctggacttat taccgcagat gcaagctttt
    27541 gaacaccgtt ttccaagagg tcgtgagcct gccgttcatc tccgagtcac aaggaacaat
    27601 ttcaaaaaac agcttctcat gtttgttgaa tattttgact tgtgaccgag gagttgacag
    27661 gagaacatcg tttattcgga aaaaaaggac tgtgacacga cttttgtcac agtctcttta
    27721 tttgtaatct aaatcatact ccgtacatct tttttctatt ttttttattt cttctaagat
    27781 cccttggcta cgaatttcat tatatggcca aagtaagact gcctctaata cataaggcga
    27841 aaaatcgctt tgattcatct cttttgaaaa actcatcgct gctatctcgc tcaatgcacg
    27901 aatgtttgat tgatgtgtat aaaaaggcaa ccgccacagc acaatttttt cgctttgcgt
    27961 ataaattccc ttaacagaag cttcgatgtg gtgaaatagt tcatgtccaa taatcaaagc
    28021 ttgcgctgtt tctttagaga tcgaagctag tcctgaacat tttaattttt ctaacggctc
    28081 cgtcatcaat cgaatccctt catcttctgc ataagttgca aaagtgattc gttcgtttat
    28141 ttgctctgat tcacaggaaa cgattggaac agccatttgt tcacaaattt ctcctacatc
    28201 ggcaaaaggg tacttttttt tgatgctaca tgccgtttcg ataccacaat cgattgattg
    28261 ttgtataaga attttctgct tggacaaagg aatctttttc caaaataaat ctcgccgaaa
    28321 ctggtagaat ccccaaattt cttcggggat ctgttgtaat tcgtttagta actcttctaa
    28381 cttatgtttg tttgatattt ccggcaacga cgatcacttc ttcctccggt tccatagtag
    28441 atacttccag atccatcaaa agctgtagtt gttccagatc cgtcgctgta aaatcagaga
    28501 ctcttgctcc taagcagaga taaaattctg gtcgggcaat caattcagtt tgataaactg
    28561 ccatatcttc ccaaagttgt tcgacagctg tcatataatt ggctgcagtt gttttcatac
    28621 atgaagcatg tccacgttgg accttgataa atcctttttg atcgatgatc cgaagacttc
    28681 cagcattttt tgaacgattc tgttcgccgc atacataaaa gaaaggtgta ttttccaata
    28741 agcaaacttc atttttattt aatcgcatat cctctgcagc aagctctagt gcttcttctg
    28801 ttgtgatctc cttggttaga tcggttgctt tcacttccgt agaaccagta gcgatagccg
    28861 ttacttttga agtctgtgga tcgatttcta cgtggacttc gatcgattct ggtgtagcgc
    28921 ctgattcaat cgctttattc atcgcttcat ttttcaacga tcgtatatct tctttacttg
    28981 gcgaagggat gatacgttct acaacatctc ggaccattgc aagtgctaca ccaatagaag
    29041 aaatcacttc ggcgttttca ggaatcgaat atttcacacc cattttatta ctaaagtaag
    29101 taatcaatga ggcagcgccg ccccctacac ctacaagaga aatctgatct ttctccaagt
    29161 gatacttgtc tgccaattct aaaatcaccg gttcaatctt agcataggat ttttccatga
    29221 tctgttctgc gatgtcttca accgtcgtat gacaataatc agctaatgct tggatggctt
    29281 ttcgtgcaga tgggacattt ccataagaaa aatgttcttc ttgcactaat ccaaggacat
    29341 tggctgcaca tgtatttgta atagtcactt cttctccgtc ttccatcact accttgacgt
    29401 aatcagcagg gtctccttct ttaggagaga aaaattcaac cttaggacct ttgatttttt
    29461 ctgtctccgt aaatactgca taatctagac cggcaatatg tgcagaacgt ggaccgacat
    29521 cgatgattcc tgactgatta gcccgtacca tcgaaccgcc tgccacacct agaacccgaa
    29581 catctaatga agagatatat gttgggtggc cggtggccac ctacaattga ataatcgatt
    29641 gctggacgtc catttttaat gaccccgata ttggttgttg ttccgcctac ttcaaaataa
    29701 acaccattag acgctctcaa gtacatcaaa gagcccatga ctgatgctgc tggaccggaa
    29761 agcatggtta aaacaggtcg ttttttcatt tcattgattt ccattactcc gccgtctccg
    29821 cgcataatca tcaaagaaac attgacccct gcttctcgta cggaatcttc tgtagaggtt
    29881 gccgtatcta gcatttttgg taaaatagat gcattgattg ctgctgtccg agttcttctt
    29941 gtcaatccat ataattttgt gatgtcagaa gccatcgttg tagggatcga ttttaccgat
    30001 gctgcttcat aaacaactcg ttctggttcc ccattatcta ctccaaatgc catagaagaa
    30061 accaagacct ccgctctttc tctttctaaa gaagaaatcg tttctgaaac acggtctaca
    30121 tttaagtgtt tgacaggtaa gaatgcattg acaatctcta tttttttctt attccctaaa
    30181 tcgatatcgt tcaatctagt ttgtcgtttt gctaaaaaac cttctaatcc accttttgcc
    30241 atcccgatta cgccgacttt tgctacgtct ccttcaatca gtgcattagt cgcttgtgtg
    30301 gtagaatgtg caacaaacac aacatcttct ggactgatat tattttccag caaacaattt
    30361 tgaaaagact gaacgacacc tgcggctact ccacggacat catcgtgggt tgttttcact
    30421 gaagattttc cgatgatttc gtgcgtcgca ttatcgattg cgactgcttt cgtatgtgta
    30481 ccgccaacat caattcccat tcgcacaaaa cgtttcattt tcttttcctc cattcatcta
    30541 atcgttttta tcgacgtttt gtttactagg cgttcccgta caataagaac gtgatcaggc
    30601 ataagatcgc tccgatgatc caaccagttg ggatggattt cttcatatat tctcgagaag
    30661 ttacttttgt ataacctaat ccccaagcaa cccaagattg tgtaatatct aaatgctgag
    30721 gagcaatcgt tgtgattgca aataacggga acaaaaaggc tggcgacatc gttggattaa
    30781 cagctaatac gacagctaaa atagctgagc ctgaaccgac tagattcatt ggaccgcgga
    30841 aaaatcctag tggtgtcaat atagcaaata cgattgccaa gactaatggt gattgcggta
    30901 tccaatctcc aaaaatagct gaaaaataag gagaagcata cgcagctgaa ttattgaaca
    30961 tcgctaaggt catcaagaaa ccaaccattg gcgcaacatc tactgcgcca tctgtaaata
    31021 atttcgcaaa ctgacgacag atttctttat aacctccgcg caattttcca gtaaacagca
    31081 atgcccagat acctgctaga ataaaaccta agatgattgg aagatctaaa agaacgaccc
    31141 ctaaaactgg taataatacg gctaaccatg agatcattgg tgcgtcgcca ccttcagctg
    31201 gtacgtttgc tgccattgca tagcgtttct ttttgttcat tgatatatta gcgaccgtta
    31261 gtaccacaac taagcttaca atcatcccaa tcataccaat ttggaaatag tcattataag
    31321 tatagctttc agccgctgga ttgaaaccag cataaatcgt ttgatattgt ttgaaattga
    31381 cgatattagc aaagattcca gccataatcg agcccataaa tgagaacagt gcaaccggta
    31441 ctgaaatacc taatgacatc aagattggca acacaataac tgcaatagaa atcactggtc
    31501 cgattcctgt catcgacatg aatataacag cggttacaat acatagtaag ctcatcgtga
    31561 ttcttggctt gtcccctcca agttcaacta cctttcgaat cagggtagct gcgattccgc
    31621 tatctactag aacacgcccg aaaaaggcac caaagaagac atttaccaag atcgatttgg
    31681 cgtactcagc tggccccgtc gtatagatat gcgtcaacac atcaattact ccttgccctt
    31741 ccatcgccgg gtttggtgaa aacgtgtttc cgactagcgc taatcccatc catacagtag
    31801 ccatgatggc aaagccgacc atcaagttgt agccttttac acagtaccag accatgaaaa
    31861 agaatgagat aactaacaaa ataccaataa taatatcact catgttattc ctccgtccat
    31921 tttgtttttc tgataagcaa actaatatat cagtcaaact cattctaact tatgtagtta
    31981 gcgctttcat taaagggtga ccataaatgt tcagttttca attaaaaaac cttaattatg
    32041 ctattttaga cttatacgtt attgtcctgt ttcatttgat ggaggattgt aaataaaaaa
    32101 agaggaaaga acataacttt tcgttatctt ctttcctctt tataattgtt tagcttattt
    32161 agccaaagct tcttttgctt ggtcagccaa tgcagtaaat gctgctgcat cgttaacagc
    32221 caagtcagct aacattttac ggttgatgtc gatttcagca actttcaaac cgtgcattaa
    32281 ttttgagtag ctcaagccat tcatacgagc tgctgcgttg atacgagcaa tccacaattt
    32341 acggaagtcg cgtttctttt gacgacgatc gcgatatgcg tagttgtatg aattcattac
    32401 ttgttctttt gctgttttaa ataaagtgtg ttttgaaccg tagtaacctt ttgctaattt
    32461 tagcactctt ttacgacgtt tacgagttac tgttccacct ttaacacgtg ccatgtataa
    32521 ttcctcctaa taaaaataaa tcttcagatt ctgttatttg tacttcttat ttcatttttg
    32581 ctagttgttg gcgaatacgt ttgaagtcgc cactagaaac catgcttgct ttacgcaatt
    32641 gacggcgttg tttttttgtt ttgccgtgga aacggtgact tgtaaacgcg cggaagcgtt
    32701 tcaatccgcc tttaccagta cgtttgaaac gttttgctga tccgcggtgt gttttttgtt
    32761 ttggcatgac tatatttcct cctcaaaatc tttcgttatc cgactatcgg ctgtccaaca
    32821 gccagttagt cattcaaatt acttactgtc gtttttcggt gccagcgtca agaacatgct
    32881 gcgtccgtcc attttcgctt tttgttccac tgttgcaata tccgcagttt cttcagctaa
    32941 gcgattcaag actttttgac caatttcttt atgggtaatg gcacggcctt tgaaacggat
    33001 agaagctttc actttatcgc ccttttccaa gaacttacgt gcattacgaa gttttgtatt
    33061 gaagtcattc aagtcgatcg taggacttaa gcgaacttct tttacattga tcactttttg
    33121 ttttttacgc gcttcgcggt ctttcttttg ttgttcgaaa cggtatttac cgtagtccat
    33181 gattcgcgca actggtggtt tcgcaccagg ggcaactaac acaagatcca agtttgcttg
    33241 ttctgcaata ttcaatgctt ctgcttttgt cttaactcct aattgctcac catctgaacc
    33301 gatcaatcgt aactctcgtg cacgaatgcc gtcgttaacc atcatatcct ttgctatggt
    33361 cattcacctc caaatattat gagagaaaga aaagcttacc gaataaacgg caataaaaaa
    33421 acgagttctc ttacagaacc cgcgcgtact tcacctaata gaaccagtga aaactatcta
    33481 gcccagtgac attatcaact aaggcgagaa gcgggtgctt ctgcttttat ttctcaacca
    33541 tcatagtata gcaaaaacta atctttttgt caatgttttt catacagttt gtcttccttt
    33601 tctgaaaatg attagattag cagactttct agcagtgatt cccgcttctt tgtgctatac
    33661 taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac ctattccacc ttctcttcct
    33721 cttcttacaa agggagagtt ttatttagaa gatgaggaga tcttctctgg tcttctgatc
    33781 aacgggggtg accaaagcta tctcgatgcc aaaaatatgg tattaagaga gagtcatatc
    33841 caaaaactta cgatgcaaaa aacagtttta cgtcattttg aatgcagtaa tgtcattttt
    33901 gaaaaatgtg atttttccaa tctggagtgg attggtggca gtttccacca agtcgtgttt
    33961 catcaatgta aattaacagg tacgaatttt gccgaaagtt atcttcgaga ctgtactttc
    34021 actgattgta ttgcttcgat ggcttccttt agtagtacaa acttgaaagc tgtctccttc
    34081 gatcattgtc aattagaaga cagcgaattt tatgaggtca cttggaaaaa cctttttttg
    34141 tctgacaatc agttgaccgg ttcgaactgg tttcgaactt ctctgaaggg attagacttt
    34201 acgactaatt tcttcacagc aatcgcttta tctcaagatt ctttggcggg actaatcgtc
    34261 aaccaagagc aagcacttgt gatcgcccaa gcccttggtt tgcagatcaa agactagatc
    34321 aacgaatccg ccgccacagt gcaataagca tccaaataga taagaccaaa ctgatgatca
    34381 aggcgattcc ccatccgaaa acggaatggg taaagggtaa ctggagattc attccgaaga
    34441 atcctgtcac aatagttgga acggtcaaga tcagcgacca gaccgtcaaa aatttcatcg
    34501 tgtcattcaa gttgttattc agcaaattat tataagtgcc agagagctgg tctaaaatct
    34561 gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc gatcagcgca tcgtccaact
    34621 cttctttttc cttttctgac aacttgcgat aaatagccaa tgccttgatt tgttctaaaa
    34681 gtactacatt ttgttttgtt cctgtaacta agtaaaccaa accgatctct aaatctgaaa
    34741 gttgcagcag attcttgttc gttgtttttt ctcttaattt cagattaagc cgctgacgtt
    34801 cactattcac ttcctcgatt aaaggaaaaa aggaatcaga aatcaaaaac aatgtacgaa
    34861 acaataaact gtatacactc atgtctggca ttcgttccaa aagcgatcga attagtcgaa
    34921 tcacgtactc ggtattcggc gtggtaaatg taaacaaacc gtcttctttt aaaataaaag
    34981 cgataggact agtttcataa tgattatcga ctttttgctg gtgcggaaca tttgatacaa
    35041 gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc ccgttcattt ttatctagag
    35101 aataagtcag catctcgtca ctaatgccat attcttcttt taagttttta acagctagca
    35161 catcgttttg ctgtacattg atccaaaaat tacggtcttg attaaaatag tgtttttccc
    35221 gcatactcta tcctcctgac atttgtttcc agtataaaga aaaaagtcgc tcaaataaag
    35281 gtttaccctt tatttgagca actgtaaatc aaatggctac tttggtttgg cgaatctctg
    35341 ctaatttttc ttcgatcatt ttcaagactt cttcttttgc ttgaggatct tccacaaaat
    35401 tatagcggtc tccatcgatt tggatttttg gactttcttt gtattcttca taccattgat
    35461 cgtaacgtct gttcaattct ttgtaataat cgtatagact tggatcaaag gacaattgtt
    35521 catacgaacg accacgtttt tcgatacgct ccaacatcgt atcaaaagat acacgaatat
    35581 gaactaataa atccggatgt tttttatggg cagcataggg aagttcttcc atcatgtttt
    35641 ccagcagttc atcatacaca cgaacttctg tttctgttgc tcttcctaga tctgcattta
    35701 gatggaaaag cagtgaatct tcataaatcg aacggtccaa tacattattg tcatctttca
    35761 ttgcttgttt gatgcttgca aaacgtttat tcaaaaaata aatttgcagc aaaaaagcat
    35821 actgttcagg gtttgcataa aataagggca agacttcatt atcatcaatc gattcataaa
    35881 atgcctgact atcaaagtgt tcagccatca tttctgttaa acttgatttt ccggcaccaa
    35941 ttgtacctgc taaaacgatc acgctcattt acctccagct cacttcatca ctaaatattg
    36001 tgtttaactc caaccttttt aaatataccc ccaatatcta gtagtgtcaa ttgtattttt
    36061 ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga aaaaagtgtt ttttctttag
    36121 aaaacgtatt cttagtttta cttctgagat ggttttagta aaataaagga ggaatgtttt
    36181 ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc aactcgtttt gggcgcttta
    36241 ttttttgctt aaaaatcact tttacgtgag aaggagttag attatggcaa actatggcca
    36301 aggagaatca agcggaaaga tcatattgat gggcgagcac gcggttgttt atggagaacc
    36361 agcgattgcc tttcctttct atgcaacaaa agtcaccgca ttccttgaag agctggatgc
    36421 aatggacgat caactggttt cttcctacta ttcaggaaat ttagccgaag ctcctcatgc
    36481 attaaaaaat atcaaaaaat tattcattca cttaaaaaaa cagcatgaca tccaaaaaaa
    36541 cttgcaactg accattgaaa gcacgattcc tgctgaacgt ggaatgggat caagcgctgc
    36601 agtcgccaca gcagtcactc gtgcttttta tgattactta gcatttcctt tgtctcgtga
    36661 aatactatta gaaaatgtcc agctttcgga aaaaatcgcc catggtaatc ctagtggaat
    36721 cgatgcagcc gctactagca gcttgcagcc gatttatttt acaaaagggc atcctttcga
    36781 ctacttttct ttgaacatcg atgctttttt gattgtcgct gatacaggaa tcaaaggaca
    36841 aacaagagaa gccgtcaaag atgtcgctca cctctttgaa acccagcctc atgaaactgg
    36901 acaaatgatc caaaaattag gatacttgac gaagcaagca aaacaagcaa tcattgaaaa
    36961 ttcaccagaa acgttggcac agacaatgga tgaatcacaa tcacttctgg aaaagctgac
    37021 aatcagcaat gattttctta atctattgat ccaaacagca aaagataccg gggcattggg
    37081 tgccaaatta actggcggtg gacgcggtgg ctgtatgatt gcattagcac aaacaaaaac
    37141 aaaagcccaa gaaatcagtc aagcacttga agatgcgggt gctgctgaaa cttggataca
    37201 aggattagga gtacatacct atgtttaaag gcaaagcacg cgcatatacc aacattgctc
    37261 taatcaaata ttggggtaag aaaaatgaag aactcatcct tccaatgaac aacagccttt
    37321 cgttaacatt ggatgcattt tatacagaga cagaagtcat cttttctgat agctatatgg
    37381 tagatgaatt ttatctagat ggcaccttgc aagacgaaaa agcaacaaaa aaagtcagtc
    37441 agtttcttga cctttttcgt aaagaagccg gcctctcact aaaagcttca gtaatcagtc
    37501 agaatttcgt tcctaccgca gctggattag cctcctctgc cagcgggcta gctgctttag
    37561 caggcgcttg caatactgct cttaagcttg gattagatga tctctctctt tcaagatttg
    37621 ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg tggtttcgtc gaatgggaaa
    37681 aaggccatga cgacttaagt tcttacgcta agccagtccc ttccgattct ttcgaagacg
    37741 atttagcaat ggttttcgtt ttgatcaacg accagaaaaa agaagtgtcc agcagaaatg
    37801 ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg ctggttagat tccgttgaag
    37861 gggatctata tcaattgaaa caagcaatca aaacaaaaga tttccaactt ctcggagaaa
    37921 cgatggaaag aaacggacta aaaatgcatg gaacgacttt agctgcccag cctccattca
    37981 cttactggtc tcctgacagc ttaaaagcga tggatgcggt gcgacaattg agaaagcaag
    38041 gcattccatg ctactttacg atggatgctg gccctaatgt caaagtttta gtggaaaaca
    38101 gccatttatc tgaggtacaa gaaacattta ccaaattatt tagcaaagaa caagtaatta
    38161 ctgcacatgc tggtccagga attgcgatta ttgaataaag ggagattttt atgattgaag
    38221 tatctgcacc aggcaaactc tatattgccg gagaatatgc agttgttgaa acaggccatc
    38281 cagcagttat cgctgcagtc gatcaattcg tgacagtcac tgtagaatct gcacgaaaag
    38341 tcggaagtat ccaatctgca caatatagtg ggatgcctgt acgttggaca agacgcaacg
    38401 gagaattagt tttagatatt cgggaaaatc cttttcatta tatccttgct gctattcgct
    38461 tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc cttttatgat ctgaaagtaa
    38521 caagtgagtt agacagttca aatggccgga aatatggatt gggttctagc ggtgccgtga
    38581 ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt gaatttatct cagttggaga
    38641 ttttcaagat tgcagcacta gccaatttag cagttcaaga taatggttcc tgcggcgaca
    38701 tcgctgctag ctgttatggt ggctggatcg ctttctcaac cttcgaccat ccttggctcc
    38761 aagaacaaga aactcagcat tctatcagtg agttacttgc cctggattgg ccaggtctat
    38821 ccattgagcc attgattgct cctgaagatt tacgtttatt gattggttgg acgggtagcc
    38881 ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc gagagaagat aaaatggtgg
    38941 cttatcagct tttcttaaaa aatagtacag aatgtgtcaa cgaaatgatc aaagggttta
    39001 aagaaaataa tgtaacgttg atccaacaga tgattcgaaa aaaccgacaa ttactgcatg
    39061 atttatctgc aatcactggg gtcgtcatcg aaacgcctgc tttgaacaaa ttgtgtaatt
    39121 tagctgaaca gtatgaagga gccgcaaaat cttctggtgc aggtgggggc gattgcggaa
    39181 tcgtaattgt tgaccagaaa tctggcattc ttcctttaat gagtgcatgg gaaaaagcag
    39241 aaatcactcc actgccgtta catgtctata gcgatcaaag aaaggaaaac cgatgaatcg
    39301 aaaagacgaa catgtttcac tagctaaagc ctttcatgat aaacaaaaaa acgaatttga
    39361 cttcgtacgg atcatccata accctttgcc gcaaatagca gtttctgatg tcgatctaag
    39421 tacacaggca gttgggttta cactaagcag cccattttat atcaatgcga tgactggcgg
    39481 aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc gtcgcaagag aagcagattt
    39541 gatgattgcc actggttcag tcagcgctgc cttgaaagtc ccttctttag ccgatactta
    39601 tacgattatg cgacaagaat atcctcacgg gaaaatcatt gccaacatcg gtgcagggac
    39661 ttctgtcgaa agagccaaag aagctatccg attgtttcac gcagatgcct tacagattca
    39721 tttgaatgca cctcaagagt tggttatgcc tgagggagac cgtgatttta ccaattggaa
    39781 agtacttatt caagaaactc aaacagccat tgacgtcccc ctcatcgtaa aagaagtcgg
    39841 ttttggcatg acaagagaaa cgctcaatga tctagctgct ttaggagttc atacagttga
    39901 tatcagcggt cgaagcggaa caagcttcac ccagatcgaa aacgcccgtc gctccaagcg
    39961 ggaactgagt tatttagctg actgggggca atcgacagtc tcttcattgc ttgaagcaaa
    40021 tgaagcagac acttctatgg aaatcctagc ctccggaggt atccgcaatg cctatgacat
    40081 atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact tcaggaaccg tactgacgca
    40141 tctgatgaat cacggcgtag aagaaactat tatactgatg aagcaatggc aagaagagct
    40201 tcgtctgctt tatactatgg tcggcgcaac gaataccgcg gccctacacc aacaatctct
    40261 gatcttttcc ggaccagtga aggattggtg cgaagcaaga gggatcgact tagtgaagta
    40321 tggaagacgc acagaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct
    40381 aaacacgtac aaacggcgga agtaaaagca actccttcgg aaataagctg aaattcacaa
    40441 aaatttgaaa agcaattttc gtgaattcct tcttatttct tggagttaaa tgcttctgtc
    40501 ccagcctctt ttttcgaata aatgatgttt aatcagctgt ccttcggtat tcgttcaatc
    40561 cgctgatttc acaacctctc cactaaacag tgtactaaag ctcttttaag gttgttttcc
    40621 gatataggca agtatgcctc cgtccacata caagacatgg ccgttaacga aatcagatgc
    40681 gctagaagcc aagaagacag atggtccagc caaatcgatt ggctcgcccc agcgtgctgc
    40741 tggcgtacgt ccaacgataa attgatcgaa tggatgacgt tcaccgtttt cttgtgtttc
    40801 gcgtaatgga gcagtttgcg gtgtagcaat ataacctggt ccaattccat tacattggat
    40861 attgtattga ccgtattcag aagcgatgtt tttcgtcagc atttttaatc cgccttttgc
    40921 cgcagcataa gcactgaccg tttcgcgtcc taattcactc atcatggagc agatattgat
    40981 gattttaccg ccgccttttt cgatcatatc cggaataact gcttttgaaa cgatgaatgg
    41041 ggcatttaaa tccacgtcga ttacttggcg gaagtctgcc gcatccattt cgatcatcgg
    41101 tgtccgtttg atgatccctg cattgttgac tagaatatca actgaaccta catcttcttt
    41161 gatctgttga accatcgctt gaaccgcttc ttcatcggtc acatcgcaga cataaccttt
    41221 tgcatcgatg cctgcttctt tataatgcgc tataccttca tcaactgatt cttgattcaa
    41281 gttattaaat acgatttttg ctcctgctgt agctaaagag cgtgcaatct caaatccaat
    41341 accatagaca gcaccggtga ctaatgctac ttttccatct aaacgaaaca tatccatact
    41401 aaattccatc attttctctc ccttatgctt attttaattg atccatttgc accatatcca
    41461 tgtctgtata ggtgatattt tctccgcaca ttgcccagat gaatgagtaa ttgcttgtac
    41521 ctacacctga atggatcgac cagcttggcg aaataatggc ttgttcattg tctacaacta
    41581 aatgttttgt ttcatctggt ttgcccatca tgtggaacac acgtgtatct tcattggcat
    41641 aatcaaagta gacataagct tccatacgac gttcatgtgt atgacaaggc atcgtattcc
    41701 aagcgcttcc tggttctaaa atcgtataac ccatttgtaa ttgacagctt tcgcatacat
    41761 ttggatggat gtattggtag attttacgtt cattcagtgt caggccttca ccagtctcca
    41821 ttggttttac ttggtcgata ctgattttta catttggata tttatgatga gctggaacag
    41881 aactaatata gaatttagct gggttttcag catcttgaga agaaaataca acatgtcgtg
    41941 tttcttttcc gatatagtat ccgtcttgtt ttttcatttc ttctttttgc ccatcgatct
    42001 cgatgaatcc tggtccaccg atattgatca cacctagttc acgacgctct aagaagtaat
    42061 caacacctag ttctttgtct aatgtgattt ctaattcttt ttcagtaggt gtcactccac
    42121 caaagatcat tcgatcgttg tgcgtataag tcaaactgat tttccctggt tcaaatacct
    42181 tttctactaa aaattctttt ctcaattgtt ccgttgaata atgacggata tcttctggac
    42241 tgtgtgtata acgtgtttcc atttcttgca ttttcttcta ctccttctat cgaatgattt
    42301 ttccagagcc gcatgctaaa aattgtgcga cttcttcttg actaaattgg ttgcagtccc
    42361 catggatcgt atgcttcaag gcagaagctg ccgtagcaaa atcaatgatt tcttgcggag
    42421 acatattgga taaaatccca tggagcacgc cacctgcaaa agcatctccg ccacctactc
    42481 gatccacgat tggggtgatt ctatggactt gggattccac atattcatgg ttcatccaca
    42541 atgtcccctt caattcattt tcacttgctg aatgaacggt acgcttggtc gaataaaaca
    42601 cctgaatatt cgggaacttt tcctgcattt tttgataata ataaattaga ggttgttctt
    42661 ctgcttctgg cgctttctcg atacctaaca aataaagagc atccattttt ccagctgagc
    42721 agatatctac taaaggtaaa agctgatgga ttacttcgct ggcttcttgc tgtgtccata
    42781 attttccacg atagttgata tcgaagctga tcaggcagcc agcttttttt gcagcttcta
    42841 tcacctgttt cgtcattttt tgccattttt tagacaaagc tggggtgatt cctgaaacat
    42901 gaaatatatc tattccttgg aataactcct ctagcgacca ttccaaatga tccatttcag
    42961 caaaacttga tccggcacga tcatagatca ctgaggcggc gcgttcgcca ctaccagttt
    43021 ccatataata agtacctagt cttgatccac cagataatac ctgtttcgta tctactccat
    43081 aacattgcaa gtgctttttg actgcttttc ccaatgcgtt atcaggtact tttgtagcaa
    43141 aaatcgcttc atgtccgtaa ttagctaaag aaattgctac gtttgcttcc cctccgccat
    43201 aatgggcagc gaatcgttca cttgaatcga tacgggttcc ctgctctgtg gacaatcgca
    43261 acatgatttc tccgagtgtc actacttttc ccataagcag ctacctcttg atttctttca
    43321 tttttgctgc atactgttgc gcgacttccg ttactttgtc aaaatctcct gtagctgcgg
    43381 gtgctaaaag gttcccacct actccgactg ttacgacacc agcatcgaac cactctttca
    43441 tattgtccag actcacaccg ccagttggca tgatattgac ttgaggcatc ggcgccttga
    43501 aggcagaaat gatacttggt ccataagcgc ttccaggaaa tagtttgacg atatctaccc
    43561 cgcttttcag cgctgttttg atctcggtaa tagtcataca tcctggtaag taaggaattt
    43621 gatacagatt gcacatctct gccgtttctt gatcaaagga agggctaaca atatactctg
    43681 cgccagctaa gatagccaag cgagctgtga cagcgtctaa tactgtgcct gcgccaatca
    43741 ctatttctgg ttggtctttg tagaatgaca aaagttcttt gattacttga tctgcttgag
    43801 ggactgtaaa agtcaactca ataccagtta gaccaccttt gatgattgcg tggcaagcat
    43861 tcaaagcttc ttctttgctt tttccgcgta ccaccgcaat cactccggcg tttttcaatc
    43921 gagataaaat atcaattttt ttcatcttct ccacctattt cctatttaga aattttattt
    43981 tataacttgg aaatctatac ccatattata cggcagaaaa gaaaaattgc aaacgctttt
    44041 ttcattattt ttgaattctt tgctcgattt cgctttttgt ttgcttgagg gcactgatta
    44101 tttgttcttt tgtctcatct gttaaacgat atttcggcat acttacacta aatgcaccaa
    44161 tgatttgttc gtttttcacg attgaggctc ctatacaaaa aatatcacgt tcgatttctt
    44221 catcgtcaaa tgcgatgtgg gtttctttta ccgttttgat ttcctttttc aaccgtaatg
    44281 cattggtgat cgtatattct gtgtaaggga tcaacggatg cttttcaaga taatcagtgt
    44341 actgctcttc actgaattct gctaatacgg ctttgcccat cgcagaattg tataatggtc
    44401 gggtgattcc gatttttgaa gacatgcgga tcgtttgatg ttttggatct agtttgttca
    44461 cgtacaagat ctcattgttg tccaataccc ctaagtggat agtctcatcg atcttttgct
    44521 gcagctgttc taaaaacggt aaagtcaact ctaccaaatc gatttgttca atgtttttat
    44581 tcgcgtagcg aataagcttt gcacctaaac gatagttttt ctctgaattt ttatttacgt
    44641 agccgatcat caacaaagta tctagtattt tcaagactgt agaagaggtc attcccacac
    44701 cctttgcgat cgtctgcaaa gagacatctt ggttttccgc taaatagtct agtattttag
    44761 aggctttgat caatacagtt ccataaggct ttttattttc catataatat tctcctattt
    44821 ctttataaga aaaattatag cggaaatacc atcaatttaa aagagatatc cagatataaa
    44881 ctaccatttc tctttacagg caatttaatg ttgaagatcc gcttcgtttt tacatcaaac
    44941 tttgatataa agcaatgatt tcttctactg tcgtttctct tggatttcct ggtgtgcata
    45001 catctcgcaa agcatcttct gcaattgctg gaatatctgc ttcttttaca cctaattcag
    45061 aaatggtttc tgggattcca accgctttgc tcaaccgatc gatctcacca caagccgctt
    45121 ctcttacatc ttctaaagac atttcagctg ctcctttaat ccctagaaca agagcgatct
    45181 cacggtactt ttctccagta tattctttgt tatatttcat gacagttggc aaaagtgctg
    45241 cacacgctac gccatgagga atgttgtacc atgcacttaa cgggtgagcc atcccgtgta
    45301 ccagtcctaa accgacattt gagaagccca ttcccgcaat atactgtcct aatgccattt
    45361 tttctcgccc attttgatcg ccatctacag aagcttccaa agaatggccg atgatttcga
    45421 ttgcttttaa atgcagcata tcagtcattt cccaagcgcc ttttgtgata aacccttcaa
    45481 tagcatgcgt catagcatcc atacctgttg atgcagccaa ttttttcggc attcccatca
    45541 tcatatcgct gtcgatgaac gcgacgattg ggatatcatg aggatcaaca caaacaaatt
    45601 ttcggtgatt tacttcatct gtgatgacgt agttgatcgt tacttctgct gctgttccag
    45661 aagtcgttgg tatagctaat ataggtaaac atggattttt cgtatcagca acaccttcta
    45721 aactaatgac gtctgaaaac tctggattcg tcaaaataat cccaatggct tttgccgtat
    45781 cgattggcga acctccaccg attgcgatca aacagtctgc ctctgcctct ttaacaaaag
    45841 ccactccatc ttttacattt tggatcgttg gattaggtac gatcccgtcg tataaagcat
    45901 aagcgattgc tgctttatct agaagttctg ttacttttgt tgctataccg tgctcgatca
    45961 atcctttgtc agtaatgaca gctgctttgc cgaatcctcg ctgctgaaat tcagggacga
    46021 tcgactcaat cgctcctttt ccaaaatacg atgtttcatt taaaatcatt cgattactca
    46081 ttttctattc ctcctaattt gtattatttt tcacaataaa aacgaaagaa ccccaagcaa
    46141 ccggttgccc agttgcttgt tgctacagtt caaagactgg catcaattcc caactgactg
    46201 gagaggcatc tggatttgtc tccatgatat cttccatgta cttccaccat ttttgattga
    46261 tagatgtatt tgccattttt tcccataact tttcatcttc gatcactaag taaccaaata
    46321 acaaacttgt actcggatca agatagatcg aatagtcgat gaccccgtgt tcacgaagtg
    46381 ctgtacgcat ttctggccaa agttcatcat gtcttttttt gtattcttct tgtttttctg
    46441 ggtaaacttt catgcaaaaa gcttttttca ccatgtcaga cacctgcttt cgtatgaact
    46501 tcctccaaat aacctgcttt tggagtgaca ccaaacgcat ctgctaaatg ccacaggttt
    46561 tcgtccgtga ttgtttgcaa gattggtcct tgcgctttga cgtacgtata gacttctgct
    46621 gccttttctg ctgtctcgat caaaccaaag acttcatcca tgtcttttcc tgacccgtag
    46681 attccatgtt gtggccataa aaccaatcga gtttctttca ttttttcagc tgttgcttcc
    46741 ccaatctcat ttgttcccgg aaccatccac ggaatgatgg aaattccttc tggaaaaaca
    46801 actaagcatt ccgtacacat ttgccatagc gtacgactaa aggaacgttc gtctaattca
    46861 tgcgtaaaac tcatcgctaa tagatgacta gcatggcaat gcataacgat ccgattatct
    46921 gggtctactt ctaaacgggc gatatgactc atgaaatgac ttggcaactc gcttgtagga
    46981 aggcattcgt tttccagccc ccacaaaagt tccagtgttt tgccatcttc agcaactcga
    47041 acgattccaa gattctcggc aggttcgctc gctacatttt taaaatatct tcctgaacca
    47101 gttacaataa aatagcatcc tgctaaacgg ctggcatcaa aaatcatcga gatgcggcgg
    47161 atcacttttt caggatccaa gaacggtgtg atctcttctt cttttagtaa ataagaaata
    47221 ttcccgccat ttctttcatc ccatcccaag cgataaagat tcgacgtcgt ttcgatcatt
    47281 tctttcacat aaggcgcttg taaaatatct atttttttca atatagccac actcctattt
    47341 tcttaattcc aacacgtctt tttgatattc ctggacttct tccagccaat ccaaaccaac
    47401 tggaacttga tttttgtaac agaaataatt ccatacatcg gcatacggga aatctttcaa
    47461 ttcttccgta taagccattc ttgaagtaaa gtcaaaagcc agctccattt ctttcaatcg
    47521 ctcaactggt tccagcatag ctttcagcag tgctttttgt gtatttctcg taccgatgac
    47581 ccaagcagcg atacggttga tcgttgcatc aaagaaatcc agaccgatat ttgttttttc
    47641 caacaattcg ttacggacta attctttagc gatttcctgc aattcatcat ccatcaaaac
    47701 cacatggtct gaatcccaac gcactggacg ggaaacatgc aacatgattc ctttgctgaa
    47761 taaagctaat gaagataatt tattggaaat cacttctgtt ggatgaaaat gtccagcatc
    47821 tagacaaaca agtttgtcac gagtcaaccc gtaacccata taaaattcat gggaaccgac
    47881 tgtataagct tcagcgccta aaccaaatag tttgctttct actgcatctt gtacataccg
    47941 ttcatctaac tcttctgtaa aaatttcatc gagtgattcc attaaacgtt cccgtggggt
    48001 aaaacgatca atcggattat ctttcatccc atcaggtacc caaaagttat tgatacttgt
    48061 ttgcccagtt tctttcccaa agtactccgc gattttgcgg cttcttttcc cgtgttcgat
    48121 ccagtattca cgaacttctt tatctggatg agccaatgta aagccgtctt tcatcattgg
    48181 atgcgaaaag aaagtcggat tgaagtcaag accgatttct tctttttttg cccactctac
    48241 ccatttttca aaatgtttcg gctcaatttc atttagatcg acagattctt ctgtatccaa
    48301 atagatagca tgaaggttca atttgtgttt gcccggtatc aacgaataag ccttctccag
    48361 atcttgtcta agctcttcag gtgttcgtgc ggctcctgga aaattccctg tcgccatgat
    48421 tccaccggtc aattcgccat ccggtgtcaa gaaacctttg acatcatctc cttgccagca
    48481 atgcatagaa attttgatgt cctgtatctt ttctagtaca gcatctgtat ctacaccgat
    48541 agttgcatat ttttcttttg cttgttcgta atttctttca atcgtgttca tacgattact
    48601 cctttgattt tattcacgta ccattccatc gtgttttgct tattttcatc tgggtaaaaa
    48661 gtttcacatg gaaacgactg tttgattaca tttcttgctt gttctatatt cgaaaactga
    48721 cctgtactga gcatttgcat ggcaacattc ccgattgctg tggcttcact aggtcctgct
    48781 tctacaatga ttccggcata atctgctgtc aattgattca gtagacgatt attcgatccg
    48841 ccccctacga tgtgcagctt ttccaatttc gttccagtta agtgttctac tttgatcagc
    48901 tcttgtgcgt aacaaagagc aagattatca tacacacaac gagcgatagc tcccgatgag
    48961 acagggactg gctgctctgt ttcaaaacag tattgctgaa tctcttctat catatttggg
    49021 ggattcaaaa atcgagggtc attgatttcc atcacttgct gcagcggttt ttcttttgca
    49081 gcaagctctg ccaattccgc ataactgtac tcgtactctt gcattcgggc aacttcctgg
    49141 atcaaccaca tccccatgat gtttttcaaa aaacgcaccg tgttatgggc accccattca
    49201 ttcgtataat tttcagaaaa agcattgcga gaaacattgg cgatctttgt ttcaatccct
    49261 aaaagcgacc atgtcccact gctgatataa ccccaacgtt ctccttgacc tggcgttcct
    49321 aatacagctg acgccgtatc atgacttgct actgtgataa aggtcacatc tggcaaatca
    49381 tattttgaaa attgttcttt ttgcagatct cctagtatcg ttcccggctc tgtcaatggt
    49441 ggaaataatt ccttcgagac accgattttt tcaagaagtt ctatttccca ttcttttgtc
    49501 cctgcattca gcagctgcat agtagatgca ttagttttct ccgtcaccat gttaccagta
    49561 aagacatacc ctaaataatc aggtatcagt aacaatttat gtgcttttgc caaattttct
    49621 ttttcttcag caaacaattg gaacaaggta ttgaaaggct ggatctgaat ccctgtctgc
    49681 tcatacaact tatctagaga atagatttca gaaaactgtt ctactgctcc attggtacgc
    49741 ttgtcccgat aagcaatcgg atcatttatc cgatcccctt gttcatcaat cagacagtaa
    49801 tcgactgccc atgtatcgat ccccgcaaaa aggcgatcaa tccctgtctg ctttaatttt
    49861 tcaaatccaa tcaaaatctc tttgataaga taatcgatct tccagcgatc gtgaccgtct
    49921 ttgttccgaa agccgttttt gaaccggtgt acttcttcca agatcaaaga accttttgca
    49981 tccctcgtac ttttcattag tcgtccgctt gatgcaccga tatcgatggc aacaaatgtt
    50041 ggattcattt ctccacctct tttactttat tgttgattag cactttatta ataaagcgtt
    50101 atcatttatt gacaatgcta tcataataca gaatcttata ttttgcttca cactattttg
    50161 gtatataatt gcattatctt gacaagttgg gagtgtttac atggattact gggaaaaagc
    50221 caaccactca tggtcggaag actcaaaaag agtgatcttg acgcctacac aaagaagcag
    50281 gaatttattt ttttatatcc aagaaatcgg ttacttcaaa gcaccaatcc cttactatac
    50341 tgaacgagca catttacctt cttacttgat aaagtttact ttgtctggca aaggttcgct
    50401 tacatataat gataatcagc aacagctaaa agctggagac ttatttttta ttgactgcca
    50461 agaatatcag cgttatcaaa cgatcagcga agaaccatgg gaaatggatt ggatccattt
    50521 gaatggtcag aacgtccaat cattttatca ggagttcaca aaagacggcc ataacacctt
    50581 ccattcaaca ggagatccat cagaaaacaa gatccaccat ttgatcacag aattgatcca
    50641 catgcaacaa ggcacgaatg cccgtaccga tttccagtcc tctgtagcta tccacgagtt
    50701 gctgaatgaa ctgattttgc aaaagtatca gctagatttc gaagaagagg atattcccgc
    50761 ctacgtttac aaactgaagc aatacttaga cgaacatttc aaagaaacga ttacacttga
    50821 tcaattagaa caacaattct tattgaataa atatcagctg agcaaagatt tttccaaatt
    50881 tatcggttct ccaccaatcg attatttgat cagcaaaaaa ctttcttacg ctaaagacct
    50941 tcttcggtat acagaattta cggttcgaat gatttccgaa gaaataggga ttgagaattt
    51001 tgcttacttt agccgcctgt tcaaagcaaa aacaggacta tcgccgagag actttcgtaa
    51061 attcagctga ttcatataat atgtacgtca ttttttcaaa atattttgaa atatatagat
    51121 aaaaataaaa taaccgcagt ttctgcttct atgtaaacaa cagcagctac tgcggttatt
    51181 ttttagtgat cattctcatt gttcatcttc aaaatgttca taatgaacca actgtttctg
    51241 tgtatcacgt ttcaaaaatt ctttaggcgt tatgccgaac tctctcttga atgctttcag
    51301 aaaatgcgga taagaagaaa aaccactttt actatataca gcactagagc tgcggtattc
    51361 tcgcaacaag tgcttggcat agatcagctt tttcttcatt acatactgat gaaacgtaaa
    51421 tccagtgtac tttttaaatt ctcgtgtgat atggtatttg ctgacaaaaa actgcttttc
    51481 catttcctct aaagaaagcg gatgagataa attttttgag atatattgta acatttgttc
    51541 cacacgctca ttaggaacta cagcattctg ttcttgcgca ctctcttctt tttgtacaac
    51601 ttgattgaga aaaatcaagt attctaccag agcttgttca tattttatgt cttcaccgta
    51661 aattttttta tcaggaggat ggtcgataaa tgataatttt tgcttcaata cttcctgttc
    51721 tatttgcaat acacgactcc tcttctctcc cattggttga aaacaagccg atagattaga
    51781 ccatggcgtg gaacgacttt ctagaaaaga aggggtgaca aagatataga cacgctcgaa
    51841 tacatctgaa gattggcgga cgatacgatg caaatcattg gaatggatga gcagcacact
    51901 tcctgctttg atcgtaaatt gtcgtccgtt caaataaaaa gtcgcttctc cctttaatgt
    51961 agcatggatc tcatagaaat catgatgatg atagatcgtt ttattttggc tgacgacatc
    52021 tttgacatga tagatttcat actgatcaga aaccatcgta ttgacttgtt caccgctgat
    52081 tttcttttgg agcatgtgcc gcacctcctt ttgttcactt atgcacaatt ttattatacc
    52141 acgttttttg taatcaaaaa gagctgcttt tatatactct tataaaaaag aagagacgtg
    52201 gcatcttcta gccccgcccc ctccattcta aatattagtt aaccaacgtc ttctttacat
    52261 tattcaagaa gacttcttcc ataacgaatc ccatcatctt ttgtttcttc aagaaccaca
    52321 tacaggtacg gacgattctt catgatgatt tctttgattt ttgtgtactc gatcacaccg
    52381 tctcccaaat ttaccggcac gattttttcc tgttcgacac gaaaatcttt caaatgaacc
    52441 gcacatatct tttcaccaaa acgttcaaaa gcttcttcta ccaattggac ttgttcttga
    52501 taatcactac ttgtgatcaa attggtcgga tctaaaatga ttcctagata gtccgaatct
    52561 actgcttgga tcagctgctc tacccttgct aaagaataca ccggatgatt caaacctggt
    52621 tctatcccca ccatcatttg gtatttttct ccagctttta ccaagcgacg gataacggac
    52681 acagcttcag caaatgcttc atctgtgaaa ttttcttccg tatattggat ttccggcaaa
    52741 acacatccag tttccgaagc aaccatcgac gcaccaaagg aagaagcata gcgaagatac
    52801 tgctcgaact ttgtcagcaa ctcttgtcgt atcgttaaat ctggatgaat catattgata
    52861 tagcaactta aaattcctac ggtcacttct tgcttttcta aaactcgttt gacataattt
    52921 cccatcccag aattgatctc atttgcgcca gaaggtattt caggaaacga aagacctaag
    52981 gcgagctgca gtgtatgaat cccttgttct tttgtttttt ctgcaagatc ttctatcgtt
    53041 tggacttcgg aaagatcatg tcctctaatt gtaatgttca acataactat tctcctaact
    53101 cagcatattg ggtagccaca tgacgatttg cgggaagtaa gtaatcaaaa acaagacgac
    53161 taagataacc ccataaaaag gaagaagcgg tttcagcatt gcctctgctt ttacgcctcc
    53221 tacacttgca ccgacatata gtcctgtacc tactggcggc gtgatggagc ctacacacag
    53281 attcatgatt gaaaacaacc cgtaatgtac aggatcaacc cctacacttg tgacgattgg
    53341 taaaaatatt ggtgtaaaaa tcaagatagc aggcgccaca tccataaaca tacccaccag
    53401 taataacaca agattgacta ataacagaat aataaattta ttatccgtta cgcccataat
    53461 caacgaacta attgcctgag gaataccggt aaacgccatg gcgaaagaca tcatggaaga
    53521 agtagccaat aagaacatga tcgtccctga cattgctact gattgaaaca acatattagg
    53581 tatatctttt agttttaccg ttttatagta aaacatagca atcaacaggg agtaaatgac
    53641 agctatcgct gaagcttcta ctgctgtaaa tatccccgtc aagataccac cgataatgat
    53701 gacaatcaat agaaggctag gaatcgcctc ttttattatt tttccagcat gagccatctg
    53761 tcctttttcg acaacagggt atttattctt tttagcaacg atgaatgcta ctagcatgat
    53821 tcctagcgcc cacaaagcgc ctacgagata acctcccata aacaaagcag agatcgaagc
    53881 accaccagca acaagtgaat acatgataaa agcagtactt ggaggaatga ccatacctgt
    53941 cggagctgac gcgatattga ccgctgtcgc aaacttccgg tcatatcctt cttccacttg
    54001 ctgagggatc aacactccgc caatcgcggt agaagcggca atggccgagc tagagatgga
    54061 accaaataaa gcattcccta acacattcgt atgagccaaa gcccccggca cgcgcccgac
    54121 gaacaacatc gcaaaatcca ctagtttctt agctatccct ccattattca tgataatgcc
    54181 cgagagaacg aaaaacggaa tagcgatcag cgaaaaacta ttgatacttc ccaccatttt
    54241 ttgagctgaa ctaaaagcag aaacatcaaa cggtataact aacaataaag tcatcatcga
    54301 tgagaatgca atacttatag caatcggcat ccctaataca agcaacacaa caaatacaaa
    54361 aaataacact aatccggctt ctattgccat tttattcctc catttttata ttttttcttg
    54421 aattgctggc ttgttgctta atgtatcgat tagattgatc aggctataga taatgatgag
    54481 tacccccgat acaggtactg acagataaac atatcccata ggaatactta aagaaggcga
    54541 gatctgcccc attgtcaatg tgacagcatt ccatccgcca tacaccatga tcatcgcagc
    54601 aaataccaga aataaacttt gaacaacaac ttctaaaatt ttactttttt tgccagttaa
    54661 tttttcactt aaaaccgtta cggctaaatg tgctttttgt cccacaacat acgcggaaga
    54721 caacatggca aaccagacaa gaccgaatcg aacaagctct tccgtgaccg tatttggatt
    54781 tcctaaaacc gttctggaaa atacttgcca taaaacaaca gcgaccatcc caacaagcaa
    54841 aacagctccc aggatctcta ataccttgtt taaaatcatt ttcatcacag ctacgctcct
    54901 tgctcttgga tcttttgata aaggtctttg taggtaggat cgtttttgaa tatatcatga
    54961 agtggggcaa ctgcctctcg aaacggtgta ttatctacat cattgaattg gactccatgt
    55021 tcttcttgtg ctaaagcttt ttcttcttca attgcttttg caaaagcttc aatttcaaaa
    55081 accgttgatt ctttcgctgc ttcttcaatg gctttttcct gctcatcagt tagctgttct
    55141 ttgatggcat cattcatgat gatgatatct ggcacacgtg tatgctcatc ataggaatag
    55201 tatttagcca caccaccatg tccagctgta tacaagacaa attcattgtt ttctgatccg
    55261 ttgatcaaat tggattgaag agacgtgtac acttcatcac ttcccatagg tacgggcgaa
    55321 ccgcctaaaa gctctaccat tttgatagcg gtttcgcttt gcatcacacg gatcttcttt
    55381 cccttcaaat cttccggcgt atggatcgga ccgtctacca tataaaagct gcgctgacca
    55441 gaatcataat aggtcaatcc ggtaaatcct aaatttttgg tcgattggta gattggctcc
    55501 attatttctt cattatccat caccttaaag aaatgttctt catcattgaa tagataagga
    55561 actgaaaaaa tcgaataatc tttagagaaa ctttccaacg ctgaggcact tacttttgtg
    55621 aaatctactg ctcctgactg tgtcaattcg atcagttcac gttctccacc cagttgtcca
    55681 tctgggaaat actggatctg tacggaacca tctgtttttt cttttactaa acgcccaaat
    55741 tcattcattg catcaattac tggctggctg ttgcttgcat aagcaaagcg caaggtaact
    55801 atctcattta catctgcatc tgcgacagtt gccacactag aattatttcc gcagccgctt
    55861 agacctagca gacctaggct aagcgaactt gctgcaaaca gcagtgatgt ttttttcata
    55921 tgggtttcct ttcttttaac aataagagac gagcaattct gtcaaacaaa gcaccgttaa
    55981 cgactgtcca taaggcattg ctgtgattcc gatattccga taaaaatcta aactgtcccc
    56041 cattcctgtc ccgattgaaa cgttttgaac ttctcctttt tcatcgattt gttcaagcaa
    56101 tccttggata gcacgataag cgacttcttt atattcttgt ggtaaataac gtttatggac
    56161 cgctttcaaa ataccgtaag caaatcctgc agttgcagaa gattctacgt aagaagaagg
    56221 atcatccaac aacgtatgcc ataaaccgct ttcagactga taagttttta atgcacgcac
    56281 ttgtgcttca agtgtttcga tcaataattt gcgcaatgca tcttctttcg ataattctaa
    56341 gatttcaatg atctctggaa tagcaatcgt caaccagcag tttccacgag cccaaaatgc
    56401 ttcggcatag ttatgatttc cttcaaacgt ccagccgtga taccacaatc ctgttcgttt
    56461 atccatcaag tatttgatat ggatcataaa ctggtaacga gcttcttcta aatattctgg
    56521 gcgattcaac agtttcccga tttttgccaa aggaagcact gtcatcatca atgtatcgtc
    56581 ccacaactga ttcttatttt ccggtccata tgtggcatgc tgtaacccgt cttcatttgt
    56641 ccgaggcatg tcattcatga cccattctgc ccattgttct aaataaggca aatagcgtga
    56701 atctttcgtg tcttcataca agtaagccat agtcaatagc ggtgccattg tattgacgtt
    56761 tttgggcgga gcgccttctt ccatacgatc ctcaaaccat tcattgacca cttgataagc
    56821 gcgaggattt tttgtcatct ggtaattttt ataaatacca tatagaccga caccttgtgg
    56881 ccaattccac actgtccagc ttttgtcatc tactttcagt ccatcaaagt ccaacaaaaa
    56941 ttcaccatta tcgtctttga tcgttgtcag gttttcaacg agcaggtcaa ttttttcttt
    57001 cactaattct ttaggtacaa aaatctcttc tttaataatc atctttacgc caccttttta
    57061 aacgctttca ttttatatcc ttaatatagc agtaataccg tccactataa atatcgcttt
    57121 ttgaacaaaa actgtctaaa attgacctaa atgttaaata acaaaagtaa ctttcagtta
    57181 cttggaaatt aaaaaaagaa cttggcgaaa caacgttttc caagttcttt ttcatctatt
    57241 ttcttatctt ttttctacgc tttcttttgc ttatccgttg taagcatcaa tgatgatctg
    57301 tttcaattca gagatcaatg gttctttcgg attagctgtc gtacattggt cttcgtaagc
    57361 taattcagcc atacggtcta cggttgtatc tagtgtttct tgtgtgactc cttgagcttt
    57421 tagactcatg ttgatgccga cagattttcc taattcataa acagccgttg ctaaagcttc
    57481 gactaattcc tctgttgttt cgcctttcag acctaagaat tttgcgatat ctgcgtaatc
    57541 tgtatctgca cggaaatagt catatttagg gaacatcgca tgtttttgtg gatcttttgc
    57601 attatagcgg ataatatgcg gtaaaagaat cgcatttgtc cgtccatgag ggatcccgta
    57661 ttccccgccg attttatgtg cgactgagtg acaaatacct aggaatgcgt tagcaaacgc
    57721 cataccagcc attgtagaag cgttgtgcat tttttcacgt gattccatat caggtgtttt
    57781 tactgatttt tctaaatagt caaagactaa cttgattgct tgtaggctca atccacgtgt
    57841 ataatctgaa gccatgactg aaacatagga ttcgattgca tgtgttaaca catccatccc
    57901 ggtatctgct gtgacagatg ctggaactga catgacgaat tgaggatcga cgattgcgac
    57961 atctggcgtc aatgcataat ctgctaacgg atatttcaca tgtgtttcac tgtcagtgat
    58021 gaccgcaaat ggcgttactt cagaaccagt ccctgatgtt gtcgggatac atacgaattg
    58081 tgttttttct ggtttgtcga tcttatatgt ccgtttacgg atatccaaga atttttgttt
    58141 tgcaccaaag aaagatgtgt ctgggtgttc atagaacatc cacattccct tagctgcgtc
    58201 cattgcagaa ccgcctccaa gagcgattac tgtatctggc ttgaagtctg ccatcagttt
    58261 cgttcctgcg tatacggtat ttgtcgatgg gttcggttca acatctgaga agacttcgat
    58321 cttcacatca tttttacgtc gtgccaatac ttcacgaact gtatcgcagt aaccgaattg
    58381 gaccatacca gggtcacaaa cgatcatgac acgttcaacg ttttccattt tctctaagta
    58441 caatagagag ttcttttcaa agaagatttt tggcggtaat ttgaaccatt gcatgttatt
    58501 tctccgtttc gctacagttt tgacattgat taagtttaca gcagagacgt tacgagaaac
    58561 agagttctta ccataagaac cgcatcccaa agtcaatgac ggaatcattt cgttatagat
    58621 atcaccgatg ccgccttctg cagatggtgt attcactaaa atacgacaag ctttcattcg
    58681 aagaccgaat ttgacatgca actcttcgtt ttctgaatgg ataactgctg tatgtcctaa
    58741 tcctccaaga ttcaacatac cttcacacaa atcaaacgca tggtccgtgt tgttagcttt
    58801 gatcatggca agaactggag aaagtttttc tcttgatagc ggataatctg ggcctacgcc
    58861 ttccaattca gcaatcagca ttttcgttcc ttttggtaca ctgattcctg ccaattttgc
    58921 gatttccatc gcagaatgtc caacgattga tggattgacc gcatattttc cttcgttcat
    58981 cactgcatct tctaattttt gcaattcatc tggttttacg atataaactt ggtgtgcttg
    59041 gaattctgct tttactgctg catagatttc tttatccacg atcacagctt gttcagaagc
    59101 acaaatcatt ccattatcaa atgttttcga aacgatcaaa tcgttcactg cacgtttaat
    59161 ttttgctgtt ttttcaatat aagctggtac gttacctgga cctacaccta aagctggttt
    59221 tcctgtagag taagcagact tgaccattcc agctccacct gtcgctaaga cgatcgcgat
    59281 acctggatgg ttcattagca tagaagtcgc ttcgatcgaa ggatgttcga tccattggat
    59341 acaattttca ggagctccgg cagcgatagc agcatcacgt accactcgtg ctgcttctgc
    59401 agaagatttt tgggcacttg gatggaaagc aaaaacgatt ggatttcttg ttttcaacgc
    59461 gatcattgat ttgaaaatcg ttgtggaagt cggattcgtt gttggagtta ctccgcaaac
    59521 cacaccgact ggttcagcaa tttcaatcaa ccctttttgg acatcttcat tgatcacacc
    59581 aactgtttta tcatgtttga tattatgcca aatatattcg gacgcataca tatttttgat
    59641 tgctttatct tcgtaaacac cgcggcctgt ttcctctaca gccatcttcg ctaatggcat
    59701 atgttgatcc aaagctgcca ttgccatttc atgcacgata tggtctactt tttcttgatc
    59761 aaagctttcc atttctttca atgctgcatt tgctttttct gccaaatcat caatcattgc
    59821 ttgtacgtca attgtcttcg ttttttcttt ttctattact ttagccatca ttatcctcct
    59881 aatgggtcat ggttatttgt taaataattc acatgtatat catacaatat ttatttttct
    59941 atgtaaacac ttttttgtga tttttttcac agttattttt aaatataaaa ttgctttatt
    60001 tacaggtgtt tgtatagaaa aa
//
LOCUS       DAZUMO010000006        54498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000006 DAZUMO010000000
VERSION     DAZUMO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.006"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..640
                     /locus_tag="ACG8ER_000393"
     CDS             <1..640
                     /locus_tag="ACG8ER_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049006.1"
                     /translation="TKKMLVKFTNIKKYRAFFLDMNILFLLLFFLIFLLTYRKCQNYY
                     VRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYP
                     GTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGM
                     RTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(880..963)
                     /locus_tag="ACG8ER_000394"
     tRNA            complement(880..963)
                     /locus_tag="ACG8ER_000394"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(927..929),aa:Leu,seq:gag)
     gene            1036..1197
                     /locus_tag="ACG8ER_000395"
     CDS             1036..1197
                     /locus_tag="ACG8ER_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049007.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            1346..2563
                     /locus_tag="ACG8ER_000396"
     CDS             1346..2563
                     /locus_tag="ACG8ER_000396"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5049008.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2747..3583
                     /locus_tag="ACG8ER_000397"
     CDS             2747..3583
                     /locus_tag="ACG8ER_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049009.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3659..4807
                     /gene="nagA"
                     /locus_tag="ACG8ER_000398"
     CDS             3659..4807
                     /gene="nagA"
                     /locus_tag="ACG8ER_000398"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS5049010.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5085..6086
                     /locus_tag="ACG8ER_000399"
     CDS             5085..6086
                     /locus_tag="ACG8ER_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS5049011.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            6105..7124
                     /locus_tag="ACG8ER_000400"
     CDS             6105..7124
                     /locus_tag="ACG8ER_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS5049012.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7226..7942)
                     /locus_tag="ACG8ER_000401"
     CDS             complement(7226..7942)
                     /locus_tag="ACG8ER_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5049013.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7911..8696)
                     /locus_tag="ACG8ER_000402"
     CDS             complement(7911..8696)
                     /locus_tag="ACG8ER_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5049014.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8687..9436)
                     /locus_tag="ACG8ER_000403"
     CDS             complement(8687..9436)
                     /locus_tag="ACG8ER_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5049015.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9734..10258
                     /locus_tag="ACG8ER_000404"
     CDS             9734..10258
                     /locus_tag="ACG8ER_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS5049016.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10274..11773
                     /locus_tag="ACG8ER_000405"
     CDS             10274..11773
                     /locus_tag="ACG8ER_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS5049017.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11861..12463)
                     /locus_tag="ACG8ER_000406"
     CDS             complement(11861..12463)
                     /locus_tag="ACG8ER_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5049018.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12567..13523
                     /locus_tag="ACG8ER_000407"
     CDS             12567..13523
                     /locus_tag="ACG8ER_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS5049019.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13828..14784)
                     /locus_tag="ACG8ER_000408"
     CDS             complement(13828..14784)
                     /locus_tag="ACG8ER_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5049020.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14958..15518
                     /locus_tag="ACG8ER_000409"
     CDS             14958..15518
                     /locus_tag="ACG8ER_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049021.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15511..16335
                     /locus_tag="ACG8ER_000410"
     CDS             15511..16335
                     /locus_tag="ACG8ER_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS5049022.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16345..16962
                     /locus_tag="ACG8ER_000411"
     CDS             16345..16962
                     /locus_tag="ACG8ER_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5049023.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16980..17663
                     /locus_tag="ACG8ER_000412"
     CDS             16980..17663
                     /locus_tag="ACG8ER_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5049024.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18346..19629
                     /locus_tag="ACG8ER_000413"
     CDS             18346..19629
                     /locus_tag="ACG8ER_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5049025.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19645..20532
                     /locus_tag="ACG8ER_000414"
     CDS             19645..20532
                     /locus_tag="ACG8ER_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5049026.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20549..21385
                     /locus_tag="ACG8ER_000415"
     CDS             20549..21385
                     /locus_tag="ACG8ER_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5049027.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21388..22011
                     /locus_tag="ACG8ER_000416"
     CDS             21388..22011
                     /locus_tag="ACG8ER_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049028.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22008..23300
                     /locus_tag="ACG8ER_000417"
     CDS             22008..23300
                     /locus_tag="ACG8ER_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5049029.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23659..23756
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23872..24453
                     /locus_tag="ACG8ER_000418"
     CDS             23872..24453
                     /locus_tag="ACG8ER_000418"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS5049030.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24456..25763
                     /locus_tag="ACG8ER_000419"
     CDS             24456..25763
                     /locus_tag="ACG8ER_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS5049031.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26179..26667
                     /gene="purE"
                     /locus_tag="ACG8ER_000420"
     CDS             26179..26667
                     /gene="purE"
                     /locus_tag="ACG8ER_000420"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS5049032.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26660..27784
                     /gene="purK"
                     /locus_tag="ACG8ER_000421"
     CDS             26660..27784
                     /gene="purK"
                     /locus_tag="ACG8ER_000421"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS5049033.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27797..29092
                     /gene="purB"
                     /locus_tag="ACG8ER_000422"
     CDS             27797..29092
                     /gene="purB"
                     /locus_tag="ACG8ER_000422"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS5049034.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29272..29649)
                     /locus_tag="ACG8ER_000423"
     CDS             complement(29272..29649)
                     /locus_tag="ACG8ER_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049035.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29905..30633
                     /gene="purC"
                     /locus_tag="ACG8ER_000424"
     CDS             29905..30633
                     /gene="purC"
                     /locus_tag="ACG8ER_000424"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS5049036.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30633..30887
                     /gene="purS"
                     /locus_tag="ACG8ER_000425"
     CDS             30633..30887
                     /gene="purS"
                     /locus_tag="ACG8ER_000425"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS5049037.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30889..31560
                     /gene="purQ"
                     /locus_tag="ACG8ER_000426"
     CDS             30889..31560
                     /gene="purQ"
                     /locus_tag="ACG8ER_000426"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS5049038.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31561..33783
                     /gene="purL"
                     /locus_tag="ACG8ER_000427"
     CDS             31561..33783
                     /gene="purL"
                     /locus_tag="ACG8ER_000427"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS5049039.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33768..35207
                     /gene="purF"
                     /locus_tag="ACG8ER_000428"
     CDS             33768..35207
                     /gene="purF"
                     /locus_tag="ACG8ER_000428"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS5049040.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35230..36282
                     /gene="purM"
                     /locus_tag="ACG8ER_000429"
     CDS             35230..36282
                     /gene="purM"
                     /locus_tag="ACG8ER_000429"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS5049041.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36279..36857
                     /gene="purN"
                     /locus_tag="ACG8ER_000430"
     CDS             36279..36857
                     /gene="purN"
                     /locus_tag="ACG8ER_000430"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS5049042.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36854..38389
                     /gene="purH"
                     /locus_tag="ACG8ER_000431"
     CDS             36854..38389
                     /gene="purH"
                     /locus_tag="ACG8ER_000431"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS5049043.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38405..39679
                     /gene="purD"
                     /locus_tag="ACG8ER_000432"
     CDS             38405..39679
                     /gene="purD"
                     /locus_tag="ACG8ER_000432"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS5049044.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLTGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39801..40715
                     /locus_tag="ACG8ER_000433"
     CDS             39801..40715
                     /locus_tag="ACG8ER_000433"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS5049045.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40848..42413)
                     /locus_tag="ACG8ER_000434"
     CDS             complement(40848..42413)
                     /locus_tag="ACG8ER_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049046.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42406..43857)
                     /locus_tag="ACG8ER_000435"
     CDS             complement(42406..43857)
                     /locus_tag="ACG8ER_000435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5049047.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43871..44887)
                     /locus_tag="ACG8ER_000436"
     CDS             complement(43871..44887)
                     /locus_tag="ACG8ER_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5049048.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45107..46195
                     /gene="chvE"
                     /locus_tag="ACG8ER_000437"
     CDS             45107..46195
                     /gene="chvE"
                     /locus_tag="ACG8ER_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5049049.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46249..47775
                     /gene="mmsA"
                     /locus_tag="ACG8ER_000438"
     CDS             46249..47775
                     /gene="mmsA"
                     /locus_tag="ACG8ER_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5049050.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47777..48982
                     /gene="mmsB"
                     /locus_tag="ACG8ER_000439"
     CDS             47777..48982
                     /gene="mmsB"
                     /locus_tag="ACG8ER_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS5049051.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49239..50216
                     /gene="guaC"
                     /locus_tag="ACG8ER_000440"
     CDS             49239..50216
                     /gene="guaC"
                     /locus_tag="ACG8ER_000440"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS5049052.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50480..51904
                     /locus_tag="ACG8ER_000441"
     CDS             50480..51904
                     /locus_tag="ACG8ER_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5049053.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(51946..52203)
                     /locus_tag="ACG8ER_000442"
     CDS             complement(51946..52203)
                     /locus_tag="ACG8ER_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049054.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            52365..53474
                     /gene="pdhA"
                     /locus_tag="ACG8ER_000443"
     CDS             52365..53474
                     /gene="pdhA"
                     /locus_tag="ACG8ER_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS5049055.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53477..54454
                     /locus_tag="ACG8ER_000444"
     CDS             53477..54454
                     /locus_tag="ACG8ER_000444"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS5049056.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17423 a         9324 c        11930 g        15821 t
ORIGIN      
        1 tacaaaaaag atgttggtga agttcaccaa catcaaaaag tatagagcct tttttttaga
       61 catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat ataggaaatg
      121 tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc tgattattta
      181 ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa gggcaatgat
      241 ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac tgtcactttt
      301 ttatccaggt acttggatag ctactttagt agtcttggca ttattactat gtttaattgt
      361 tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta ttttgatgat
      421 aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg cttccggaat
      481 gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg cagttaaaga
      541 gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat taaccgtgct
      601 tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa atccctcgct
      661 ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca cttgcaagtt
      721 tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta catgattgta
      781 cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa cgttcaaaca
      841 aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg ggactcgaac
      901 ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt ccaacacatc
      961 tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag ctcaaaaatg
     1021 ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg aatgatctta
     1081 ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct aggaatcatt
     1141 tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga caattaagtt
     1201 ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt ggcaaaattt
     1261 tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa gttctaactt
     1321 tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca aattagaagg
     1381 tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc aagaagaggg
     1441 cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg aagcaccaaa
     1501 cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact cagattccaa
     1561 ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac attttccgaa
     1621 tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca cgatgtgtat
     1681 gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact atccgttgtg
     1741 gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg atgaagaggc
     1801 agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga caaaagctat
     1861 tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat tattagaaga
     1921 aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa tctatgatcg
     1981 cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg atttgttcgt
     2041 tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag taggctggat
     2101 ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga atatgttgtc
     2161 ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg cgctaggcgg
     2221 ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac aaagagaata
     2281 tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa aagctgcttt
     2341 ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg aaaaattcgt
     2401 tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat ttaattggaa
     2461 ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga aacaaacagc
     2521 taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa aaccaaattt
     2581 ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga gttttcttaa
     2641 ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac ttcctaaagt
     2701 aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg aaacgttaat
     2761 cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga cgcaaaaaat
     2821 gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata atgaggaact
     2881 gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga ttgatcatgt
     2941 gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac tgttggatag
     3001 ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt ctgatatttc
     3061 tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg gtgtttgcga
     3121 attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag cattaagcta
     3181 ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga ttagttgtat
     3241 cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt tgagtcgaag
     3301 catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt cgcaaatttt
     3361 ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt tagaacaggt
     3421 gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca atgatttatt
     3481 tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag cattctatga
     3541 aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt agaccattct
     3601 tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag agaatagaat
     3661 gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac atgcaggcta
     3721 tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg agggagaagt
     3781 caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata cacatattca
     3841 cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag tgatgtctga
     3901 aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt caagcaaaga
     3961 acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac caggagctaa
     4021 aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag gggcacaaaa
     4081 cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag aagcttcagg
     4141 tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag agtttgtcaa
     4201 acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta cactagaaga
     4261 agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca atggtatgcg
     4321 tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag aacatgtctt
     4381 ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa tcctaatgga
     4441 aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg gtgggatgcc
     4501 tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa cagcacgtct
     4561 agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta aaaatgttgt
     4621 agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga ttccagcaat
     4681 cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg cagactttat
     4741 cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg aacgtttcca
     4801 cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt tttaaggttt
     4861 tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt aatttcctat
     4921 tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat tagcaaaata
     4981 aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg aacttatttg
     5041 acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact tcaacagttt
     5101 ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa tctgttggaa
     5161 aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt cgagctttca
     5221 tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt tggggattag
     5281 ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat aaaggggata
     5341 agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc ggaacgccta
     5401 tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc aatattggct
     5461 accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag atggaagcta
     5521 aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc ggccttttca
     5581 tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa ggagagataa
     5641 gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag ccgatggatt
     5701 atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca ttaggtgaag
     5761 taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta gtcaaagtgg
     5821 tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg aatattctac
     5881 cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca gctgcaacag
     5941 tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc aatgcatcct
     6001 tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta ttacaaatga
     6061 ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga atcgcatgtt
     6121 acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac cattatgatc
     6181 tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag gaccatgatg
     6241 ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa atgcaggaat
     6301 atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat ctacaagcgg
     6361 cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat gcgatagcag
     6421 agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg acgatccgaa
     6481 catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc agaaattgca
     6541 cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg tttaagggac
     6601 taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa aaagtattga
     6661 cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt catgtacctc
     6721 atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa atgaaaaaag
     6781 gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct attttaagag
     6841 ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat gaaacagagg
     6901 tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc cagcaattag
     6961 tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca gatgaggcat
     7021 tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa actggaaaaa
     7081 cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac atggatattc
     7141 gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta tacttggact
     7201 attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc cgcccattct
     7261 ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt ctttcgttca
     7321 atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt gacagtgagc
     7381 ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata aatgcactta
     7441 ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat ggtatttcgt
     7501 gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct ttttcagtcg
     7561 tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa cgttgcaaag
     7621 tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt atggtttcga
     7681 tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt ctaagccaag
     7741 ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc agatctgcgt
     7801 ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg attacgttca
     7861 tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga tcatcttcga
     7921 ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag atagacgatt
     7981 gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt gacgattgaa
     8041 tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc tagcgtaaat
     8101 attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt cacgatgaat
     8161 acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct ttcatgtttt
     8221 tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa aagtgaatgg
     8281 atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag catttcaaca
     8341 gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc aggtactttg
     8401 atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg ctggataaaa
     8461 tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg aagtttattc
     8521 atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc cccaagctcc
     8581 tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc taaaaaacgg
     8641 tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa atacatggat
     8701 tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc atatagatta
     8761 tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag aaaatgtttt
     8821 ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag attccttcat
     8881 ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct tgtggttcaa
     8941 gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg atgcctacgc
     9001 taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact tgattgtcaa
     9061 tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca taataaatag
     9121 ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa aattcttgtc
     9181 ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg taacttgggt
     9241 ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct aaattcgatg
     9301 caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca gtctgcagct
     9361 ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt ttatctgaca
     9421 cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa acaatcaaag
     9481 aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt aatggttttt
     9541 tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat tttaacatga
     9601 aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa aacaccactg
     9661 agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg tagaaatgga
     9721 aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt atacattgct
     9781 gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg attttgatgg
     9841 cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat ttggttttct
     9901 aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg gaggcatatc
     9961 gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac atgccgaatc
    10021 aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga tcacaccgat
    10081 ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca ttagtcgtcc
    10141 agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag tattgatcat
    10201 tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa aagcttaaag
    10261 gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg cagctttgat
    10321 tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac cggatgaagc
    10381 gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg aaagcaataa
    10441 caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc aacgttcgaa
    10501 tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt atacggaaca
    10561 aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat ctgtagaaga
    10621 gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg aaaatgaagc
    10681 aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg tagaagaaat
    10741 ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg tagaccttgc
    10801 aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca aaaacggtta
    10861 tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag atatagcaga
    10921 agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag aatcacaagc
    10981 agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag aactgaaatt
    11041 agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag cctacaattt
    11101 agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta aaatcgtaga
    11161 acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa agcaatatga
    11221 ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag aagcacaagc
    11281 tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg aagcactggc
    11341 agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag cagcactagc
    11401 aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca aggaatatgg
    11461 cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc gcgaagcagc
    11521 acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca gttcaggaga
    11581 aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt ctactcaaga
    11641 aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt tgtcgacacg
    11701 tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac aaaactcgat
    11761 gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa gactttttaa
    11821 agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg ctatttttct
    11881 tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat cgttctttct
    11941 tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca ggaggacgaa
    12001 gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt acacgttgga
    12061 tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac aacgtagcta
    12121 ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc acaataataa
    12181 ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag cttttgacaa
    12241 aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta gactttccaa
    12301 aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat gcagcaatca
    12361 tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca gctaaggcac
    12421 ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt ctcctttttg
    12481 gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt attcaagtat
    12541 aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc aaaacagtga
    12601 tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat tggccagctg
    12661 cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg cgtacgggca
    12721 tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt ttttttgctg
    12781 ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg cttgcctctc
    12841 gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat tatttagtgg
    12901 aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg attggattag
    12961 tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg aaggaaaacc
    13021 aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa tttcttttat
    13081 cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc attcgcgaaa
    13141 gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg ctatactacg
    13201 aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg ctggaaaatc
    13261 tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta ttgatttata
    13321 ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt attgaagaag
    13381 tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc gaggatcgag
    13441 cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag gcagaattct
    13501 cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg tcaaaaagct
    13561 gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc acaagtcaca
    13621 atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc ggagctgaac
    13681 ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc ggtaataagt
    13741 ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt acttgatgca
    13801 gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt gcatttgatc
    13861 caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt caccttcaat
    13921 catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc cgctttcgat
    13981 gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg ggtaatcagc
    14041 aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt ttgcctgaaa
    14101 agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga cggattgttc
    14161 gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt gagagctcaa
    14221 aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc ctccagctaa
    14281 ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga aaggtgcttg
    14341 gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact gctgataaag
    14401 tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt ctgctaagcg
    14461 catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata gaacgtgttt
    14521 gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta cagcaatgta
    14581 cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag cttttggaat
    14641 attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa ctgctgttag
    14701 ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac caagaccaac
    14761 gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat tgagtttctt
    14821 gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact tgaaacatta
    14881 aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag tagaatagtt
    14941 aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt gtattattag
    15001 aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt ccagaacaat
    15061 ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag aaagtaaaaa
    15121 atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa acgattattt
    15181 ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca cgcatcgcgc
    15241 aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa aataatcagc
    15301 aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg ggctatgatg
    15361 atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga gaaaaaatcg
    15421 aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag gcgaatgggg
    15481 aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt tggagcagtt
    15541 gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc gcatgttcaa
    15601 gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa agaaaagaac
    15661 agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac gtccaaacaa
    15721 ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga tcacaaatta
    15781 gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata cttgatcgac
    15841 aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc tggatttcca
    15901 ttagtgatgg tctcagctta ccggtctgta tcatcccaac aacaagtttt cgaacaaaat
    15961 gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg aggcgacaaa gattactaaa
    16021 gaaacgatta cggagccagg atacagcgaa caccacacag gtctggcagt cgatgtcgtc
    16081 gatcaaaact ggtacaacag ttatccttct actgtattgg atgcaagtta cggagatcag
    16141 cctggagcta aatggatcgc agataatgct gcgcagtatg gatttatcgt tcgttatcca
    16201 aaaggacacg aagatataac taagatcaca tatgaacctt ggcacttacg ttatgtcggt
    16261 aaagaaaacg ctgagtatat caccaaacac caattgacgc tagaagaata tctgaatgaa
    16321 ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt ggaaaaagca aaagcgcaag
    16381 aatatccgtt ggccagcagt ggttgccggt gtagcagtga tactcttagc ctttgttttt
    16441 tccttgaaga atctgtcttc tccttttact gataatcagc aagaaaatga agcgcaatcc
    16501 catcagcaat ttattgaacg gcttgttccg catgcccaag aactacaaga tggttatgga
    16561 gtgcttccta gtatcatttt aggacaagct atattagaat caaattgggg aaaaagtacc
    16621 ttggctagca aatacaataa tcttttcggg atcaaggctt atggggatca gaagaaagta
    16681 agcctggaaa caaaggaatt tgttaacgaa gaatggataa ctatccaagg agacttcaaa
    16741 gtgtatgatt catgggaaca gtccatggat gaccatacac aactgttcgt ccaaggagta
    16801 gattggaatc ctgcgctgta tgaaaaagtg attacggcca ccaactatca ggaagccgca
    16861 caagcattgc aagatgcagg ttatgcgaca gaccccggtt atgcgcaaaa aatcatccag
    16921 gtcatcgaga cgtatcaatt gaatcagtat gaccggagat agcaaaggag aagtggctga
    16981 tggcagaaca atttacgcca gaaatcatga cggaattgag aaaagatatc gtaagagtac
    17041 cggaaatcat tttagaagca agcgggatca agatatttgg aaaattgatc cgttcgatca
    17101 tctttactac ggatattgcg atcatacgta atacaaatgc gaatgcagta attgctgtgt
    17161 atccttttac acctcatcca gcgattacta aaagcattat tgaggcagct gacattccag
    17221 ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg ttcttcctac atgagcatgt
    17281 tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg tcctacgcct ttagagacag
    17341 taaaagaagt ttgtcgagta gttgatattc cagttatcag tacggtaacg tcgaaatata
    17401 cagaaataga tgaaaagtta gcagctggta tttcgattat caatatcagt gcaggtaaag
    17461 aaactgcaga agcagtacgc cattttcgta aaaaatatcc tgaacttcct atcattgcga
    17521 caggtggtcc aacggaagaa agtattgctg agaccattca agcaggagca aatgccatca
    17581 catatacgcc accaagtagc ggagagcttt ttagtcgtaa aatggaaaaa tatcgggaaa
    17641 ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag acgaggaaga gcagatagtg
    17701 gctcttcctc gtcttttgta ctccagtact atttgttcag aattcaaaaa aaagagaaat
    17761 ttttttgttt ttttacctgt aatgtacata agaagagtta aaaccttttg ttattattag
    17821 cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt ctatagaatg aaaaacgaat
    17881 aaaaactaaa aaaggcaccc gacaatttag tagcagctaa attgtcgggg cttgaaaagt
    17941 cagcgggaac tgaccaatca agttgccata gtcaatgctt gtgcatcgac gtgtatattg
    18001 tacctaattt catacagaat tactagttat ttttctgttt tttagggaag agcgcacacg
    18061 aagaagcatg cttatttagc tatgtcaagg atattgggca gttgtctgat atcctttttt
    18121 ctttgcgtaa ttatcctccc atcattgaag caagaagtgt ttttagcgat gaaagaagaa
    18181 tcagcactat ctaatttttc ttgaaagtat tgagttgttc atcctaggca gaacgataag
    18241 gcaagtcttg gtttttattc cccttgaaaa agatcgttta ataaatatgt tctgtataaa
    18301 aatacagtat attgaaaaat aacaatggaa gtagaggtgc agagaatgaa gaaaagaatg
    18361 agaatcatct tagctttaag tgttatgtta ttcttcttga cggcttgtgg gcaagaagat
    18421 gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc aagtccgaca cgatgcaaca
    18481 gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg tgacgatcga ttatgaattt
    18541 tatgattatg aaggatatta cacaaaatta ggtacactag cagcatcaga tgatatctgg
    18601 gacattttcc aactgaacaa tcaatttcct caatacatca atcagattga accattagat
    18661 gaatatgtgg agcaagggat aattgatatt tctaatgcag atgaatccta tttagcaacg
    18721 acgacttacg acgggcaatt agttggactg tctaacgggg tgaattcgtt tgcgattgct
    18781 tacaataaaa atatatttga acaattaaat atccctgaac cagcttcaaa ttggacatgg
    18841 gatgaatttg aacaaatcag ctatcaaatc actgaagaat taggaatcta tgctatttca
    18901 agattcgaag attttatagc tggctgtatt attcaaatac cacaagttga gaaaggattg
    18961 aatttttata atcgggaaga tctatctaac agcttgggat ttgaaaatcc agactatcta
    19021 acagattttt tccagctgcg taaaaatctg gtagacaaag gggcccatcc agaaccaggt
    19081 ggtggagctg caagtacgac ggatctgtct tttcaggcgg ttagggatag agaagcggca
    19141 atggtgtttc tatcaagtaa ccagttgaca gaaatattgt caggggctcc tgaagatatg
    19201 gagattcagt tgattaatcc acctcgcaga aaagcagatg gagaatctgg tatcccttta
    19261 cgttcttcgc aaatgctgag tatggcaaaa agctcagccc ataaagaaga agctgctaag
    19321 tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa ttttaaaagg agaacgtggt
    19381 gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg cagatgatgc aactttgcaa
    19441 gcgacatttg attatttaga aatgatttcc gatatggaca acggcgaagt gaatgtcttt
    19501 gatagtgctt tgaatccgga aattcaagac caatacaatc ttttagttga aaaagtcatt
    19561 tatgaagaag taacagcaga agaagcagca gagagtcttt atgagtttgc gtcaaacatt
    19621 ctaggatagc agcaggagga aggcatgaaa aaagaaaaac taaaacggtt cttggatcaa
    19681 gacaatgtgg tcggttacgt atttgcttct ccattcatcc taggatttct attgataaca
    19741 gtgataccga tgatcttgtc actttattat tcgttcaccg attatcgttt gggaaatcca
    19801 gtctcatgga tcggcttgga taattatata aagatgctga gtgatgcccg ttttttgaac
    19861 tccatccgag tcacttttct ctatgtattt gcatctgtac ctgtgaaact gatctttggt
    19921 ctattgattg cttatctttt aacgaggaat atcaaaggaa cgactttctt tcgttctttg
    19981 tattatgtgc ctagtttgat cggtggcagt atttctatcg ctctagtttg gcgggagttg
    20041 ttttctaaaa acgggttagt gaatattttc ctggaacgat taggcgcacc tgcgctttcc
    20101 tggtttagcg atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc ttggcaattc
    20161 ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga tcccaggctc gtattatgaa
    20221 gcggcagaga tcgatggagc gaacaaatgg cagacgtttt acaagatcac gttacctctt
    20281 ctttcgccga tcattttgta taacttggtt atgcagacaa tcgcggcgtt tatgactttt
    20341 acgcaggcat ttatcattac ccaaggtggt ccgaatgatg ccacgaactt ttatgctctt
    20401 tatgtgtata accaagcttt ttcttttaat tctatggggt atgctagtgc tatttcgtgg
    20461 atcatgctgt tgattatggg agctgtcacc tttctgattt tcaaatcatc aaaaaaatgg
    20521 gtcgttgtgt gaatgagagg aggaagaaat gaataataca gtaacgaaat ggtttcaata
    20581 cggttttatt ttgctattag gtctagtcat gatttatccg gtcttatgga tgatcgcagg
    20641 ttcttttaaa acggatcaag agattatgag tggttcgctg aatttgattc cagagacttt
    20701 tcaatgggaa aattacgcaa aaggatgggc ggggtttgct ggaatttcat tttttacttt
    20761 ctttaggaat tcgtttgtca tctctgcagt ttcgacgata gggacagttc tgagctcgac
    20821 ttgtattgct tacgcattat cgagaatcga tttcaaagga aaaagattct ggttcgtcgt
    20881 catgttagta acgatgatga ttcctgcaca agtgattttg attccgcagt ttattattta
    20941 caatcggctg aatttggttg ggacgtatgt gccgctgatt ttaccacatt tttttggaca
    21001 agcatttttc atttaccaga tcatgcaatt catggtcagc atccccaaag agttagatga
    21061 atcagcgatt attgacggat gcagcaagta tagtgtcttt accaaaatta tttttccttt
    21121 gctgaaacct tcgatcatca cgacgatcat catccagttt tattggaaat gggacgactt
    21181 catgggacca ttgatttatt taaacaagcc gagatcttac acggtatcga ttgccatcaa
    21241 attatttgca gacgctggat ctagtacgac ttacgcgagt atgtttgcga tgtctacgtt
    21301 gtcgctgcta ccggtttttc tgattttctt attcttcaat aaatatcttg tccaggggat
    21361 cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa tagaagagat gtttctttac
    21421 aagctcaact actatgtctg tcggattttt ttaaccagtc ttatcagtaa tgctcttttc
    21481 gctttagttc taggatgttt gttcacttta ggattagtgc tgtctgctcg tgttgtctat
    21541 ctgatttatt ttttgatgat gtatccttct tattatctat ctgtccatta cttggcagaa
    21601 aaaaaggtat gttggaggaa aggaaaaatc aaacggatgt gtttcatcat tacgttgttt
    21661 cagctgtttg tcattatttt tgcagttgat tggtatttat tagtgggaaa gattggagct
    21721 tggtatctga ttccttttct attgtgtctg ttgctttttg tatcccatag tttttttagt
    21781 acgctaattg atatctgcaa agaagataaa gcagatagtc ttgtttattt gatcaaaagg
    21841 aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg ccttttgtat
    21901 ggactgttct ggtgtataca gcatgatttt ttactcactg cattactgta tccagggctg
    21961 ccatttgtca cagtattttg cttaaaagaa aaggagaatg actttaaatg aagaaaaaat
    22021 atgtacaagt cggacttggt gggagagcgc gtttttttta tgaggcaatt gcctctacgt
    22081 atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg atggattatg
    22141 caaatcggat acttgtgaac aagtatggac agaaaaaagt aaagacatgt atgccatcac
    22201 aatttgagca actactggat gacgtacggc cggctgaagt cattattacg tcagtagatc
    22261 gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt atttgcgaaa
    22321 aacctctgac tatcgatgag gaaaaagctc aagaaataat aacggtccaa aaacagacgg
    22381 gaagaagcct gcgagtcact tttaattatc gttatgcacc gtatcacacg aagataaagg
    22441 aactgataga aagtgatgtg attggtgatg tgttttctat ccacttcgaa tggctgttaa
    22501 atacgcaaca cggggcagat tatttcagaa gatggcatcg aaataagcac aatagcggcg
    22561 ggcttcttgt tcataaatct acccatcatt ttgatctagt gaatttttgg ttgaacagcc
    22621 gtccccaaaa ggtatttgca ttcggtggat taaactttta tgggatgcat aatgcggaga
    22681 aacgcggagt agaaatattt tacagtcgat gctatggaag cgaactagct aaagctgatc
    22741 catttgctat agacctgtca gccaatgagg aattgcgaga aatgtattta gaagcagaaa
    22801 aagacagtgg atatattcgt gaccgcagtg tattttccga tgatatctct attgaagata
    22861 cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg ctaaacgcgt
    22921 atatgccgtg ggaaggattc aatgtttctt tcaatggcag caaaggacgg atcgaaatga
    22981 aagtgatcga aaagtcttac atcaatgcag gtggagataa aaaggatgaa ggagcagccg
    23041 ttcttcagtc catccatgtc tatccgatgt tcggtgagcc atatgaggta atcgtcgaac
    23101 aagaagcggg cggacatggc ggaggagatc cgcgtttatt agatgatcta tttggtgaag
    23161 aaaaagaaga agatccgtgg aatcgggcag caactcacgt agatggtatt ttgtcgattt
    23221 tgacaggaat cgcagcaaat cattctattg cttcaggaaa agcagtagtg attgacgagc
    23281 ttgtcagctt aaaagaataa gaatagattt acggaaatta cttcccgtag tttataaaat
    23341 cttgccttat tgccgatagg ctgtcttttg aacactattt attcggaaaa aggggctatg
    23401 acatgctttt tgtcatgcct tctttttatg tgggctgctt ttttagatag gagaaaataa
    23461 aaataactat ttaaacccga acaaaaagga ataatgcatt aaaaatcatt agattttaca
    23521 aaaaaataag taaaccacgt tgtcagtttc agaaagtagt gttagactgt tttcaatatt
    23581 tgaaacaaca ttttttattc aaatatggca aactgccttc acgtgcaaaa cagttgcaag
    23641 aaaatgaatt tacactatta ttagttttac atacctactt atatatcgtc ataatatgga
    23701 tgacagtttc tagccagtac cgtaaatgct ggactataag taaaagattg gctatttcac
    23761 ccttcttttc ttatgataaa gtggagggtg tttttcttta acagaaacat agtaggtttt
    23821 tgcacaaaag cggttatttc ggtattaata aatgataaag gagcaaataa agtgaaagaa
    23881 ttagttgaac gtattcaaaa agacggacgt atattaggcg agggcgtatt aaaagtcgac
    23941 agcttcgtca ctcaccaagt agaccctgta ttgatggaac aaatgggaaa acggtttgca
    24001 gaagtattta aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc tgggatcgca
    24061 ccagctttat ttgctgcaag agaattagat gttccaatga tcttcgctag aaaagcaaaa
    24121 agtttgacga tggatgaaga attattgaca gcttctgttt attcttttac gaaacaagtg
    24181 acaagtacga tttctatttc tcgtaaattt ttatcttcag aagacaaagt attgattatc
    24241 gacgatttct tggcaaacgg acaagcggca aaaggattga tcgaattatg ccagcaagct
    24301 ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga tggacgtcag
    24361 ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc cttgacgaat
    24421 ggacaagttg agtttcttga ggaggacgca taatcgtgga aaaagaaaca caaaatggaa
    24481 aagctgcggt tttagggctg cagcatctgc tagcgatgta tgcaggagca gtagcggtgc
    24541 ctcttttgat cggtacagga ctaggcttta atcaagaaca gatgacttat ttgatttcaa
    24601 ttgatatctt tatgtgtgga ttagcgacat tattgcaatt gacagttaac cgatttttcg
    24661 gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca ctgatcttga
    24721 tcggaaccaa tgaaggagta ggagctatct acggatctat tatcgcttct gggatctttg
    24781 ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact attccctcct atagtaacag
    24841 gaacggttat caccgttatt ggtttgactt tgattcctgt agcaatcgag aaaatgggtg
    24901 gaggtaatgc aacagcagct gattttggtg acaaaacaaa tctgctactt gcatttgtga
    24961 cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt tcaatcgcgg
    25021 tattgattgg tttagtcgga ggaagcatct tagctgcttt catgggaatg attgatctga
    25081 gcgcgattgg agatgcgccg ttgttccatg ttccacaacc cttttatttt ggtaaaccaa
    25141 ctttcgacgt atggtcgatt ttactaatga tcatcatttc catggtcagc atggtagaat
    25201 caacaggtgt atattttgca cttggtgaca ttactgggaa aaatgttgga gaagaagaat
    25261 tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt aggcggtatt ttcaatacat
    25321 tcccttatac tggcttttct caaaatgtcg gattggttca actttcggga atcaaaacaa
    25381 gaaaacccat ctatttctct gcattcttct tgattgtttt aggattattc cctaaaatcg
    25441 gtgcgttagc acaaatcatc cctgagccgg tacttggtgg aggaatgtta gtcatgttcg
    25501 ggatggtggc agtccaagga atccgtatgc tgttggaagt tgatttcagc aatgataaaa
    25561 atctattgat tgttgctgtt tctgttggat ttgggctggg tttcaatgtt acgccgacat
    25621 tattcagtca aatgccgcaa acattgcaaa tgttcactgg aaatggcatc gtaatgagta
    25681 gcattacggc tatcatattg aatttgatct ttaatggatt gaaaaaagat gaagaaatat
    25741 tggaagaagc cttcaagaag taatagagca aaagaaaggt tgtgaaatca gcgttttgac
    25801 ctgattgtta gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt tgtttgagct
    25861 aataccgagg gtcagctgat tgaacaccgt tgaaggaaga ggtcgtgagc ctgccgttca
    25921 tctccgagtc acaaggaaca atttcaaaaa aaagcttctc atgtttgttg aatattgttc
    25981 cttgtgaccg aggagttggc aggagaacac cgtttatcca taaaaaggaa tgtgacctaa
    26041 cttttgtcac attccttttt tttatcttta ataaacacga acaataaaat gtttttcttt
    26101 gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat gaatgtagaa
    26161 aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag tacttcagat
    26221 tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc ttatgaaaaa
    26281 aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca aaacgctcga
    26341 aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct gccgggtatg
    26401 gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg aacattaaat
    26461 ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt agctactaca
    26521 gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat gctttccatg
    26581 tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc taaaactgtt
    26641 atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga tcgggatcgt
    26701 cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg gctttaaagt
    26761 aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt ggcatattga
    26821 aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag atgtcattac
    26881 ctatgaattc gaaaacgtca gtgtagaggc gttaaatgcg attttaccca tgtcatttat
    26941 tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa aatctttttt
    27001 ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga cggatatcca
    27061 agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag gcggctatga
    27121 tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca tggacctttt
    27181 aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga tttcgatcat
    27241 ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa acatccacca
    27301 caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg tgatcgagga
    27361 ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg ttttaggggt
    27421 agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc ctcgtcctca
    27481 caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg cccatattcg
    27541 aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg cagtaatggt
    27601 caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata agccagactg
    27661 gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg gacatatcac
    27721 tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga atatttggga
    27781 ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga atatctggac
    27841 ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg aagcttgggc
    27901 agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg catcatttga
    27961 tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg cgtttacacg
    28021 agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc taacaagtac
    28081 ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata ttttacggga
    28141 agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca aatatacagt
    28201 gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat tgaaacttgc
    28261 cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg caaaaggagt
    28321 agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc catttattga
    28381 agagtatgta tgtaaacaat tggggatccg tccacaagaa atttctactc aagttctccc
    28441 tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa gtattgaacg
    28501 ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag aagaattttt
    28561 tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga tcggttcaga
    28621 aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct atgaaaatgt
    28681 cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca tcttgcctga
    28741 taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta aaaatctaac
    28801 ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga tttatagcca
    28861 gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt atgatcttgt
    28921 tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc tgttagaaga
    28981 agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct ttgattatca
    29041 tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat aaagaagagt
    29101 gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa gcagatccgt
    29161 tagcggttct gcttctttta gtttattata ttttcttatc aataatagga acctgatttt
    29221 aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga attaagcgta
    29281 aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca agttttcgag
    29341 aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat gatttttgaa
    29401 acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg tctgataaag
    29461 aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt ttcggaatgt
    29521 ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca aatcattata
    29581 tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt gttttattgc
    29641 atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt aattctaata
    29701 taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc gtttctgata
    29761 aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt gatttataaa
    29821 aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat ttgcttttaa
    29881 tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga aggaaaagcc
    29941 aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt agatcaagca
    30001 actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt gaataatcaa
    30061 atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca ttttattgaa
    30121 caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct ggaagtggtt
    30181 atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga aggtaaagaa
    30241 ttcatcaaac caatcattga attctactat aaggatgacc agttggatga tccatttatc
    30301 aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag tgagctgaaa
    30361 gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt cggtttggtt
    30421 ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct tgcggatgaa
    30481 gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat ggacaaagac
    30541 gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata taaccgtcta
    30601 tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta tatgtaacct
    30661 ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc catcgtatgg
    30721 gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa cttgctgatg
    30781 cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt gcaaatgtaa
    30841 atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat gaaatttgcg
    30901 gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt tcgcgagatc
    30961 atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg atttgatggg
    31021 gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc gattgctcgg
    31081 ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc agtattcggt
    31141 acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt actgagacga
    31201 aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc caagacggcc
    31261 tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca tggggaagga
    31321 aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca gattgccttt
    31381 acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac gaacaaagca
    31441 gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt gctaggatca
    31501 gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt gaccgtatag
    31561 atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat ctatgccgaa
    31621 tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg aagaatgcct
    31681 aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc ctataaaaat
    31741 tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca aggccctgga
    31801 gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa agcagaaagt
    31861 cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt tggcggaatc
    31921 atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc attgaggttc
    31981 ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc aggtatcggc
    32041 ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt tgatccttgt
    32101 tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca caaacatatc
    32161 cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc aaaaacaggt
    32221 cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga agaagaacag
    32281 caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct tgaagcgtgt
    32341 ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat gggagcagca
    32401 ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact tatattgaca
    32461 ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat gttatcggaa
    32521 tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat ccaagaacta
    32581 tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga cgggcaatat
    32641 cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt agctgaagat
    32701 gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt tcagcagtta
    32761 gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct gctgcagcaa
    32821 ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt acaaaccaat
    32881 acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac gaataaagcg
    32941 ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa aatcggagga
    33001 caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa gcccttggcg
    33061 atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg ggaattatcg
    33121 acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt tatttcagga
    33181 aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc aatgattggt
    33241 atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa aaaatcaggg
    33301 gatctaatct atatattagg taagacgttt gcagattttg atggcagtga attacaaaag
    33361 atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat tgaaaagaga
    33421 aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc tcatgactgt
    33481 tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca gctagggata
    33541 tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc aagatttgtg
    33601 ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga tgctgctgtt
    33661 ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga cggtcagatc
    33721 aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg tcttatgaag
    33781 tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca gacgccgcta
    33841 gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt gcaggtatcg
    33901 tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt tctgaggtat
    33961 tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga catgtacgtt
    34021 atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc aaatttcatg
    34081 atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc cttagaaaag
    34141 agttagagga aaatggggcg atcttccatt ctaactccga tacggagata ttgatgcatt
    34201 tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct ttgaatgaag
    34261 tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc gctttggacc
    34321 caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac gtgattgcct
    34381 cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc cagcctggtg
    34441 agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag gacacgcagc
    34501 tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac atcgcaggga
    34561 tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa tcgccagtaa
    34621 aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc ggttatgcgg
    34681 aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt gcccgaacat
    34741 ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta tctgctgtac
    34801 gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt cggggtacga
    34861 caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc catgtgagga
    34921 ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa acgcgtaaag
    34981 aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt gcagattctt
    35041 taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa gatgctccgt
    35101 attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta tatgactatg
    35161 aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag cagaccaaga
    35221 agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga tgtcgaagct
    35281 ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg tttaggtatg
    35341 atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca attaaaagag
    35401 ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc aatcgaagca
    35461 gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga tatcttagca
    35521 caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa tgatccgtta
    35581 cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa tatggcgctg
    35641 attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta tgatctagcc
    35701 ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac gatcaaccca
    35761 ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta ctctcttgtt
    35821 cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc agaattgaaa
    35881 aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc cttgcttccg
    35941 ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg gtttgtagaa
    36001 aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg ctcatggccg
    36061 attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat ggagatgtat
    36121 gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa agtaaaagaa
    36181 atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa agtgatcgcc
    36241 aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct gtttttgctt
    36301 ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa ggattggaat
    36361 cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa cgtgcaactg
    36421 cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa aaagagtatg
    36481 aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta ttagctggat
    36541 atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc atcaatatcc
    36601 atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc tttgaagctg
    36661 gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat actggaccaa
    36721 tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct ttagaagaaa
    36781 aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt attgaaaatg
    36841 gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg ggattgtagc
    36901 attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag gaacaaagca
    36961 tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag ggtttcctga
    37021 aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc ttttaggaag
    37081 acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac caatcgactt
    37141 tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg tcgaaatagc
    37201 agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg cagcaaaaaa
    37261 ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac tttcagaatt
    37321 acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa aagtctttcg
    37381 tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg tggaagaaaa
    37441 agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt acggtgaaaa
    37501 tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt cgatcgcagc
    37561 agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg cagatgctgc
    37621 gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac acatgaatcc
    37681 ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt atgaagcaga
    37741 tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc tgccaacagc
    37801 agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata cacaagaagc
    37861 tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact ttgcgcagga
    37921 agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg tacaagatca
    37981 agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta agccgactcc
    38041 agcagaagca aaagcattag tgtttgcttg gagagctgtc aaacatgtga aatctaatgc
    38101 gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga tgaatcgagt
    38161 cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg cagttttagc
    38221 tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac atggtataaa
    38281 agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata tggcgaataa
    38341 atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga gaggagtcgg
    38401 ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa gtaaaaaact
    38461 tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg ggatgacaaa
    38521 agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa tccattttgt
    38581 taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt taaatgggat
    38641 cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag cagcagctct
    38701 gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa tcccgacagc
    38761 taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag aaaatggtgc
    38821 gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg tggctaccac
    38881 agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat ttggggatag
    38941 tagcaaaaaa atcgtcgtag aagagttttt gacaggagaa gaattctccc ttttggcgtt
    39001 tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc gggcatttga
    39061 taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac cgcaaatttc
    39121 aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt tgggaatgaa
    39181 agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta cgaaggaagg
    39241 accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag tcgttctttc
    39301 tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc gtacagtaga
    39361 gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg ccggttaccc
    39421 cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc tttctgttta
    39481 ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac gtctttattt
    39541 agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca ccgcgttaat
    39601 ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag ctgccaatag
    39661 cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac aaacataatc
    39721 ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt tgttgtgtat
    39781 ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat cagaaagcag
    39841 ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta aaagtgattg
    39901 agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat ggaaacggtg
    39961 aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga gaagatcttt
    40021 ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct aaaaaacata
    40081 tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg gatgctttac
    40141 ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg tttgatgaat
    40201 tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca atgcaaatcg
    40261 aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa tttggcttga
    40321 aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa gcaagtatcg
    40381 atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc caagcatggt
    40441 caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa ttccctgaat
    40501 taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact ggcttggctt
    40561 ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg atgaacgtag
    40621 ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa gattggtacg
    40681 aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca ggtcaaaagg
    40741 gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag atattctgtc
    40801 tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta gttatttttg
    40861 tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt tttcttaaat
    40921 aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc tgaaatacga
    40981 tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt cagataatcg
    41041 atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg acttaatgca
    41101 acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc tttgataaaa
    41161 tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga gggcagagca
    41221 gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt
    41281 gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt cttttctttc
    41341 aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg tgttcgagtt
    41401 ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt tttcgataat
    41461 gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag tttcgcttct
    41521 tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt ttcaagtatc
    41581 atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac aggaccaatg
    41641 gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt ttgggctacc
    41701 tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa tttgatgaat
    41761 cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa cttcaaataa
    41821 tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc taataatacg
    41881 gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa tgtttcactg
    41941 atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca
    42001 agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac tgctttttct
    42061 attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc tgcctgtacc
    42121 tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat gagacgagtc
    42181 caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg
    42241 atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc catctcacca
    42301 tctccagctt cgccgacata agtgattgga taataagaac caaggacagt tatttgttcc
    42361 cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata catgtttgtt
    42421 tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc cttctcggta
    42481 tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga gacgcttgtt
    42541 tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg ctaactcttt
    42601 acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa ctttgaattg
    42661 gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac ggacgtactt
    42721 cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta aaatagaaac
    42781 aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt atcttatttt
    42841 taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa tccagtcttg
    42901 tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc
    42961 ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat aaataaaatg
    43021 gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt attgtttcaa
    43081 agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc tagcggtaag
    43141 tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag
    43201 ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct ctaaaaagcg
    43261 acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca ctattgttaa
    43321 tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga tctccacacg
    43381 aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct gattgatgat
    43441 ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct cgttttgata
    43501 atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt ctttaagtgt
    43561 atttgtatta tttttgctct ctcgaatatc attcctaagt tcttctagga tattttcctc
    43621 attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt ccaaaacttt
    43681 ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg tctgttgata
    43741 aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa cagcaaatag
    43801 aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat
    43861 acttctccag tcaataattt ctggttggga tgttagctat attctgctgg gaaaagacag
    43921 tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt ccaaaataac
    43981 gagtaacggt attggctaca taccagccga catcaggttt acactcgact acacaattga
    44041 cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt acaatgatca
    44101 aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg tgagtcattt
    44161 catcactatg gctgtataat acatcgattt cggataaatc atttgtttct aaatatcgtt
    44221 tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga acaatagtca
    44281 tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga ttgattgatt
    44341 ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca gcaagttcta
    44401 ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat aaacccgccc
    44461 gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg
    44521 tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct tcttttaata
    44581 cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact ttataggcaa
    44641 tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt cggtagagaa
    44701 cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt ttccgccagc
    44761 tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat
    44821 taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa aataaaacac
    44881 gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga taaaagggaa
    44941 aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca
    45001 aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt
    45061 ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga aaaacaaatt
    45121 tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg
    45181 caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc cgaccaaatc
    45241 agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa aactgggtta
    45301 taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac aaatagaaaa
    45361 tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt cggctttaac
    45421 tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg atcgtttact
    45481 gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag taggtgtatc
    45541 gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac cattcacgat
    45601 tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca acggagtgat
    45661 gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg gacagacgag
    45721 tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa ggatggataa
    45781 tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc catatgatcc
    45841 aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc
    45901 tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga tcattgccgg
    45961 ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata cgattgaaat
    46021 gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa cttatgataa
    46081 tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata aagataatta
    46141 tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa attaagagga
    46201 accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat ggcggattgt
    46261 attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg
    46321 aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa
    46381 tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga cggggaaatc
    46441 ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc
    46501 gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga gaatattttt
    46561 ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga aaagaaaaca
    46621 tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt atcccagatt
    46681 ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc cgtccgtctg
    46741 ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga
    46801 ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca taagctgaat
    46861 gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac gattgagaca
    46921 ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg cagagattta
    46981 gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa agtagaagac
    47041 tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat caacttgcat
    47101 ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag aactgaattc
    47161 gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat attaaaggaa
    47221 ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact cgcttatgtc
    47281 tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga aaacgtgacg
    47341 ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa agaagtaact
    47401 gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca tcaaattgtc
    47461 agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa
    47521 cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc aaaatatgag
    47581 atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct tatctcttct
    47641 gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga aggacgtatt
    47701 acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt aatgacgaaa
    47761 gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag agaaaaaact
    47821 aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga tcattttagc
    47881 gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc cattgaatat
    47941 aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga tgttattagt
    48001 tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg ttggtgcaat
    48061 tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta tcgcatgtct
    48121 tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg cagggatacc
    48181 ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac aagttgttct
    48241 aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta cgggttattt
    48301 accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag gaatgatcat
    48361 cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa atcatttatt
    48421 tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag tcgttgtact
    48481 aggaataact tatatctttg cctcttatca agggtatccg attattttga tcattcttgg
    48541 tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc gacagattta
    48601 tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa agcgaatcac
    48661 gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag ttttagctgc
    48721 tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg caatggcggc
    48781 agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg gtgcgattgt
    48841 cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg tcggggtaga
    48901 ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag acttgtataa
    48961 taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac tgtgacaaga
    49021 catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt tgtatagact
    49081 aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct tgattttttt
    49141 gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat taataatgtt
    49201 cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt gattacgaag
    49261 atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt gatacaacgg
    49321 tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg cagacgatta
    49381 ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc atgcatcggt
    49441 ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga ttgattacct
    49501 cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg gcagaagaat
    49561 cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac tcagttattg
    49621 atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc ggtaatgttg
    49681 gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca aaagtcggga
    49741 ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact ggaggctggc
    49801 aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc gctgacggtg
    49861 ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca atggtcatga
    49921 ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc gaaaatggaa
    49981 tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa aaacgaaatg
    50041 ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca ctagtggaaa
    50101 tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg gaagccattc
    50161 gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca atctaaaaaa
    50221 taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata agaacagcca
    50281 aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag atacaagctt
    50341 ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag tcccttatgt
    50401 tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag aaaaaataga
    50461 caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac cgatttatcg
    50521 tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc ctggaagtca
    50581 acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca ccgttgtcca
    50641 tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca gcggatggtt
    50701 tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc ggcaaatgat
    50761 ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag caaagcagat
    50821 ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc ttttgccgaa
    50881 ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag aaatggatct
    50941 tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta aatttgaatt
    51001 ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa tctttttgat
    51061 tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt ctttttttta
    51121 ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga tggatgaaga
    51181 agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga aagcagtgct
    51241 tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac gacgagaaaa
    51301 gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg tatttacgga
    51361 tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc cggataatgt
    51421 gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg gttgggtgat
    51481 aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg agttttcgat
    51541 gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg atttgaaaat
    51601 gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt ggggagattc
    51661 acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga ttacttggga
    51721 tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc ttggaattgc
    51781 accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca gtaggctgga
    51841 tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt ggttaacaga
    51901 ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa tttcaagctg
    51961 tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac cattatttgt
    52021 acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta taacaccaac
    52081 gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa atgcagatga
    52141 aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata tgcggattgg
    52201 cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca taaatccatg
    52261 ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt ttcaatcgca
    52321 gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca aacaaaaaag
    52381 caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca gtatatcaag
    52441 cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta tccgatgatg
    52501 aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa cgttcaacag
    52561 cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc
    52621 agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt tatcgggatg
    52681 tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg tctcgaggac
    52741 atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa attatcatcg
    52801 gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga aacaagaaaa
    52861 atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa
    52921 tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac aacgggtatg
    52981 cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg
    53041 cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta tatactgtat
    53101 ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc gaaacactga
    53161 cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac cgtacaaaag
    53221 acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa tatttgacgg
    53281 aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa
    53341 tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca gatttcttga
    53401 aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga
    53461 aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag atgccttagc
    53521 acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg
    53581 aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc gagtattcga
    53641 tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt
    53701 tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg acgaagtagt
    53761 agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac ccatcacaat
    53821 tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata atttagaagg
    53881 tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt atgatgcaaa
    53941 aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag aacacatgaa
    54001 actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc ctttggataa
    54061 agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag caatggtgcg
    54121 tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg aaatcattga
    54181 tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg aaaaaactgg
    54241 tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc aagtagtttc
    54301 tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag tttctgcacc
    54361 tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg caaaagatat
    54421 cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat
    54481 ggcttatcaa tttaaatt
//
LOCUS       DAZUMO010000007        53771 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000007 DAZUMO010000000
VERSION     DAZUMO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.007"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACG8ER_000445"
     CDS             <1..730
                     /locus_tag="ACG8ER_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5049057.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            788..2827
                     /locus_tag="ACG8ER_000446"
     CDS             788..2827
                     /locus_tag="ACG8ER_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5049058.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLLAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2936..4087)
                     /locus_tag="ACG8ER_000447"
     CDS             complement(2936..4087)
                     /locus_tag="ACG8ER_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5049059.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4241..4771)
                     /locus_tag="ACG8ER_000448"
     CDS             complement(4241..4771)
                     /locus_tag="ACG8ER_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5049060.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4870..5220)
                     /locus_tag="ACG8ER_000449"
     CDS             complement(4870..5220)
                     /locus_tag="ACG8ER_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS5049061.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG8ER_000450"
     CDS             complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG8ER_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS5049062.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG8ER_000451"
     CDS             complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG8ER_000451"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS5049063.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7623..8285)
                     /locus_tag="ACG8ER_000452"
     CDS             complement(7623..8285)
                     /locus_tag="ACG8ER_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5049064.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8469..10115
                     /locus_tag="ACG8ER_000453"
     CDS             8469..10115
                     /locus_tag="ACG8ER_000453"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5049065.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG8ER_000454"
     CDS             10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG8ER_000454"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5049066.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11296..12849)
                     /locus_tag="ACG8ER_000455"
     CDS             complement(11296..12849)
                     /locus_tag="ACG8ER_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049067.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12997..14232
                     /locus_tag="ACG8ER_000456"
     CDS             12997..14232
                     /locus_tag="ACG8ER_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS5049068.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            14205..14285
                     /locus_tag="ACG8ER_000457"
     CDS             14205..14285
                     /locus_tag="ACG8ER_000457"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049069.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14411..15490)
                     /locus_tag="ACG8ER_000458"
     CDS             complement(14411..15490)
                     /locus_tag="ACG8ER_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5049070.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15593..16033)
                     /locus_tag="ACG8ER_000459"
     CDS             complement(15593..16033)
                     /locus_tag="ACG8ER_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5049071.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16090..16500)
                     /locus_tag="ACG8ER_000460"
     CDS             complement(16090..16500)
                     /locus_tag="ACG8ER_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5049072.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16680..17114)
                     /locus_tag="ACG8ER_000461"
     CDS             complement(16680..17114)
                     /locus_tag="ACG8ER_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5049073.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17214..18062)
                     /locus_tag="ACG8ER_000462"
     CDS             complement(17214..18062)
                     /locus_tag="ACG8ER_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5049074.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18451..20217
                     /locus_tag="ACG8ER_000463"
     CDS             18451..20217
                     /locus_tag="ACG8ER_000463"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS5049075.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20319..21578
                     /locus_tag="ACG8ER_000464"
     CDS             20319..21578
                     /locus_tag="ACG8ER_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5049076.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21725..23026
                     /locus_tag="ACG8ER_000465"
     CDS             21725..23026
                     /locus_tag="ACG8ER_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5049077.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23029..23880
                     /locus_tag="ACG8ER_000466"
     CDS             23029..23880
                     /locus_tag="ACG8ER_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5049078.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23893..24708
                     /locus_tag="ACG8ER_000467"
     CDS             23893..24708
                     /locus_tag="ACG8ER_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS5049079.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24859..26025
                     /locus_tag="ACG8ER_000468"
     CDS             24859..26025
                     /locus_tag="ACG8ER_000468"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5049080.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26255..26902
                     /locus_tag="ACG8ER_000469"
     CDS             26255..26902
                     /locus_tag="ACG8ER_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5049081.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26986..27930
                     /locus_tag="ACG8ER_000470"
     CDS             26986..27930
                     /locus_tag="ACG8ER_000470"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS5049082.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28065..29516
                     /locus_tag="ACG8ER_000471"
     CDS             28065..29516
                     /locus_tag="ACG8ER_000471"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS5049083.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29537..30373
                     /locus_tag="ACG8ER_000472"
     CDS             29537..30373
                     /locus_tag="ACG8ER_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5049084.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30373..31032
                     /locus_tag="ACG8ER_000473"
     CDS             30373..31032
                     /locus_tag="ACG8ER_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5049085.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31158..31847
                     /locus_tag="ACG8ER_000474"
     CDS             31158..31847
                     /locus_tag="ACG8ER_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049086.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31853..32809
                     /locus_tag="ACG8ER_000475"
     CDS             31853..32809
                     /locus_tag="ACG8ER_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS5049087.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32853..33365
                     /locus_tag="ACG8ER_000476"
     CDS             32853..33365
                     /locus_tag="ACG8ER_000476"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5049088.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33621..34169)
                     /locus_tag="ACG8ER_000477"
     CDS             complement(33621..34169)
                     /locus_tag="ACG8ER_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5049089.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34258..35439)
                     /locus_tag="ACG8ER_000478"
     CDS             complement(34258..35439)
                     /locus_tag="ACG8ER_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS5049090.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35675..37183)
                     /locus_tag="ACG8ER_000479"
     CDS             complement(35675..37183)
                     /locus_tag="ACG8ER_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS5049091.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37342..37533
                     /locus_tag="ACG8ER_000480"
     CDS             37342..37533
                     /locus_tag="ACG8ER_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049092.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37736..38653
                     /locus_tag="ACG8ER_000481"
     CDS             37736..38653
                     /locus_tag="ACG8ER_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS5049093.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38646..39719
                     /locus_tag="ACG8ER_000482"
     CDS             38646..39719
                     /locus_tag="ACG8ER_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049094.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39867..40385
                     /locus_tag="ACG8ER_000483"
     CDS             39867..40385
                     /locus_tag="ACG8ER_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049095.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40366..40758
                     /locus_tag="ACG8ER_000484"
     CDS             40366..40758
                     /locus_tag="ACG8ER_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS5049096.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40834..41769
                     /locus_tag="ACG8ER_000485"
     CDS             40834..41769
                     /locus_tag="ACG8ER_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5049097.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41902..42681
                     /locus_tag="ACG8ER_000486"
     CDS             41902..42681
                     /locus_tag="ACG8ER_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5049098.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42674..42991
                     /locus_tag="ACG8ER_000487"
     CDS             42674..42991
                     /locus_tag="ACG8ER_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5049099.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43235..44845
                     /locus_tag="ACG8ER_000488"
     CDS             43235..44845
                     /locus_tag="ACG8ER_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5049100.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45002..45850
                     /locus_tag="ACG8ER_000489"
     CDS             45002..45850
                     /locus_tag="ACG8ER_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5049101.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46081..46560
                     /locus_tag="ACG8ER_000490"
     CDS             46081..46560
                     /locus_tag="ACG8ER_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS5049102.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46593..48821
                     /locus_tag="ACG8ER_000491"
     CDS             46593..48821
                     /locus_tag="ACG8ER_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5049103.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            48950..50593
                     /locus_tag="ACG8ER_000492"
     CDS             48950..50593
                     /locus_tag="ACG8ER_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5049104.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50666..51211)
                     /locus_tag="ACG8ER_000493"
     CDS             complement(50666..51211)
                     /locus_tag="ACG8ER_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS5049105.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51234..51719)
                     /locus_tag="ACG8ER_000494"
     CDS             complement(51234..51719)
                     /locus_tag="ACG8ER_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5049106.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(51709..52521)
                     /locus_tag="ACG8ER_000495"
     CDS             complement(51709..52521)
                     /locus_tag="ACG8ER_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS5049107.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(52660..>53771)
                     /locus_tag="ACG8ER_000496"
     CDS             complement(52660..>53771)
                     /locus_tag="ACG8ER_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS5049108.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        17906 a         9785 c        10381 g        15699 t
ORIGIN      
        1 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
       61 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
      121 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
      181 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
      241 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
      301 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
      361 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
      421 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
      481 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
      541 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
      601 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
      661 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
      721 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
      781 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
      841 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
      901 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
      961 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     1021 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     1081 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     1141 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1201 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1261 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1321 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1381 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1441 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1501 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1561 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1621 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1681 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1741 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1801 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     1861 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     1921 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     1981 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2041 tgctcctgga tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat
     2101 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2161 atcttttcaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2221 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2281 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2341 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2401 atatggacag ggcgaacttc taattaatcc tatccagcaa gcagcaatgt attctgtttt
     2461 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2521 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     2581 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2641 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2701 gcttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2761 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2821 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     2881 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     2941 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3001 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3061 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3121 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3181 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3241 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3301 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3361 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3421 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3481 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3541 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3601 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3661 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3721 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3781 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3841 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     3901 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     3961 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4021 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4081 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4141 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4201 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4261 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4321 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4381 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4441 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4501 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4561 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4621 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4681 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4741 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4801 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4861 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     4921 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     4981 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5041 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5101 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5161 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5221 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5281 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5341 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5401 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5461 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5521 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5581 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5641 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5701 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5761 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5821 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     5881 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     5941 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6001 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6061 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6121 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6181 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6241 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6301 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6361 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6421 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6481 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6541 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6601 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6661 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6721 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6781 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6841 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     6901 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     6961 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7021 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7081 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7141 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7201 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7261 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7321 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7381 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7441 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7501 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7561 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7621 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7681 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7741 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7801 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7861 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     7921 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     7981 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8041 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8101 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8161 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8221 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8281 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8341 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
     8401 atagcgattt ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga
     8461 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
     8521 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
     8581 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
     8641 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
     8701 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
     8761 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
     8821 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
     8881 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
     8941 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
     9001 ttcttgctgc gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
     9061 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
     9121 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
     9181 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
     9241 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
     9301 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
     9361 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
     9421 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
     9481 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
     9541 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
     9601 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
     9661 caaatattcc tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
     9721 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
     9781 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
     9841 gaaaaccatt catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg
     9901 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
     9961 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    10021 aaatgttcat caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact
    10081 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    10141 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    10201 attgatggat ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga
    10261 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    10321 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    10381 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    10441 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    10501 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    10561 atttgaattg ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc
    10621 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    10681 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    10741 ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg
    10801 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    10861 agaaaatgat gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc
    10921 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc
    10981 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    11041 cgaatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    11101 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    11161 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    11221 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    11281 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    11341 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    11401 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    11461 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    11521 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    11581 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    11641 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    11701 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac
    11761 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    11821 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    11881 aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac
    11941 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    12001 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    12061 tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata
    12121 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    12181 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    12241 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    12301 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    12361 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc
    12421 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    12481 ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    12541 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    12601 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    12661 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
    12721 atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag
    12781 aaaaatatct aattttgtat tgcttttttt cttcaagaga aacgttttaa tcgcttcttg
    12841 agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt
    12901 tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat
    12961 ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata
    13021 tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag
    13081 atagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag
    13141 gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc
    13201 atcaaagcaa aaattgacta ttcaggagac ttagcaccta tactacaatc agctgttaca
    13261 tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg agaacacttt gactatccaa
    13321 cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg
    13381 ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatcattt aacgccgtat
    13441 agtcagcggc agacaattga gcaagattca gctatcgact caggaggaga tcttgttgaa
    13501 gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac agaaaatcca
    13561 gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga aactcaacca
    13621 gagccagaca atgaatcaaa acctgaaata acccctggag aaaaaccaga tgtagaccca
    13681 gaagaaaaac cagatgtgac tccagaacca gatacggatt ctggaaacca aacagtaccg
    13741 gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga ggtagcccca
    13801 gaagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca aacagtaccg
    13861 gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga ggtagatcca
    13921 gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca aggaatacca
    13981 gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa aaaatcaaat
    14041 ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat atttgatagc
    14101 gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt cctgaaacga
    14161 aaaataaaca gctaccaaaa tccggagagt cacaaaacaa agttatactg tggagcggaa
    14221 tcattctatt aagtatagca acaatgcttt ctgcaaaaag atttaaacaa aacagatctc
    14281 tctaacattt gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa
    14341 tgaccccaaa ataccagatt gaaatctgat atttggggtc gttcatactt tatctttctt
    14401 catatttatt ttaagggata tctgtttctt ttggaagatg gttcgggatc tttttcactt
    14461 tcaatacatc taaaaagaag gtaaagatcg gaatgccgac aatcaagccc caaacgccaa
    14521 agaaccgttc gctaacaagt aagaccacaa atgtataaaa aatcggcagt tctgttttgc
    14581 tggacatcaa cttcggattc agcacataag actcaattag atggacaatc aaaatcgtaa
    14641 tcaggatata aacaacatct cggatgcctc cttgggaata agctatgaaa gatagaggaa
    14701 tacaagaaat gataacacct gctactggga tcagacttaa aatgaaaatc atgatcgcta
    14761 aactcagcag ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc aacgtattta
    14821 aaagagcgac aacaaattga gcttctaata ccaaaccaaa tgtattgaca aacttatcgg
    14881 caaaatagta aatatcttga aagaaccaag aaaattcact ttttaaaaac aatcttgaga
    14941 atacaattgt ttttttcttt tcaatcataa aaaagaaact taagatgaat gacatagcaa
    15001 acgccactgc catttttcct atatcctgta aatagccaag cgcaatcgaa gcgccatttt
    15061 gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg gatatattgt aaaacaggat
    15121 ctgcatcttt tggctgatgc tcgtaaaaat taataacgga ctggatcatg tcaaatgttt
    15181 gatgaataag catcgggata tactttgtga atgccagata gataaggtaa acgatcagtg
    15241 tataaatcat gatagctaaa acacctgtag ggattcggac aaatcgctga atgatttgaa
    15301 caagatgaat catcaaataa gtaaagataa atgtgagcaa aatggttgta atcatacttc
    15361 taactaagaa aagaacaaaa acgacaaata ataaaacagt gagacggcgt attttttcat
    15421 tcgccaaaaa acgctgataa acggtcaaat cgacaacctc caaataacaa aaaataaaca
    15481 cgttattcat tatatgatgg aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa
    15541 tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag tttcagccct
    15601 ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc agtgtatcca
    15661 attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct tctgcttgtt
    15721 tttgcaacgc agcaccttga gcagtcagcg taacatttac gattctttca tcacccgttt
    15781 cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga gtcagcgtac
    15841 cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc ttttcccata
    15901 aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg aggggagtgt
    15961 agtgtctaat gatttcttta gaggcggcat ataaaggaaa acatacttga ttaactaaat
    16021 atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag cggatttaca
    16081 caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag ctgtagcttt
    16141 tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt ccagagaaag
    16201 accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac tttctgaata
    16261 aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct gtttgatcaa
    16321 ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt cagggttggt
    16381 tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac gatcgggatt
    16441 atgcacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca tttttttcat
    16501 aaaattacca cctttaatta tattttatac aattgaattt tacacaatat aattgtggaa
    16561 agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca gacgcgcttt
    16621 ctcgaatagc aaacatatac aaaaaatatc agagacaaaa aataatctca tcataaacat
    16681 tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg atggacctga
    16741 tgaagcatcc ataaataacc aaaaggatct ctgaacattg agttagtcac accaaagtcc
    16801 atcatttctg tcatttcttg aatctctaca cagccatttc gtaatgcttt attatgtgtg
    16861 tcctcgatat cagagacgat gatattgaac cagatcgtat ttggatgttc agcagtagga
    16921 gcagtcaaac caagagacgt gttttcatcc agcatatgga atctcgtacc atacaaggta
    16981 aaaatgactt cattattgcc tctagggtaa tctgttgcct caatcagttg tatttcatca
    17041 aagattgttt gatacagttc tagtgctttc aaactatcat ttaatacaaa atcgatttcc
    17101 gttcctttat gcataaagat ctcctctcct tttcagtcga acagttacta gattctttta
    17161 ttataccagc taaagaaaac gcattcaaaa aaatagcgga tttacttacc aactcattcg
    17221 attggatgat aagtaaatcc gcgtcccatt acttctgcta cgctgctgac aatgacaaag
    17281 gcacttggat cgatttcttc tatcaatggt ttcattcggg aaaattcatg atcagtcaca
    17341 acgatcaata aaacctcttt tgactttcga ttatacccgc cttttgcctc tagcagggta
    17401 aggccacgtc caatctcagc acttaagcga aggcggatgt cttccaaata atgttcactc
    17461 atgaccataa cagatttctt tcgattgata cctgtttcga tataagtcat gacaattgaa
    17521 gtgattgcaa tagataaaat agcataaaga aactcttcta ttccaaaaat aaacagatta
    17581 agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat
    17641 tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag aagagttgtt tttatataaa
    17701 atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa
    17761 gtaatcattg tttctggaac tattgcaatg attaaaggaa aaaccaagct tccgaaaagt
    17821 actttaaaaa agggtttttt acctaaaaat agaagagcta gtaccaacat aacgatattc
    17881 aacactaaaa taactatagc tcggtcaaag cctaaagcag cttccagtaa tattcctaga
    17941 ccgctgacac ctccagcagc aatatgatga ggccctaaaa acatattgat agcgactcct
    18001 aatatgaaga gggcaaaaat aatcttaatg tatgtccata taaattgtct cgtttctttc
    18061 atttattcac ttccttaatt cattaataat actcttatct tattataact tacttttcac
    18121 cttaagttaa ctttaattaa taaaaaaatt aggctagtct aaattattat gtttcattct
    18181 tctagaatat acttctacgc caaaaactgt taaaaaactt ttactttaga aaattggaga
    18241 aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga ctaaaacttt
    18301 taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa
    18361 ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact tatagtgatg
    18421 acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta tcatcgaccg
    18481 gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct tcgtactagg
    18541 aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat aacaactgaa
    18601 aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt tcatgattat
    18661 tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca tgttgttggg
    18721 gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg agaaaatgtg
    18781 attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga tcgattcact
    18841 attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag atttgcaaac
    18901 ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga tccagaccga
    18961 gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta cttgacggac
    19021 cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa tcataaatac
    19081 gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact tttaaaaaac
    19141 ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt attcaatcat
    19201 atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa atcgatctat
    19261 aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac tgacttacca
    19321 gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat gccgaaacta
    19381 aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta ttggataaaa
    19441 gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca tcatttttgg
    19501 aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct tggagagatt
    19561 tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat gaattatgca
    19621 tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc acaaatggtc
    19681 agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg aacattcaac
    19741 ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa caaagaattg
    19801 atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat ctattacggg
    19861 acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat gatatgggag
    19921 aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc tctaaggaaa
    19981 cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa tgatcgtgaa
    20041 gataagctct atttcacacg gaaactagag ggacagatca tctatgttta ctttaatcag
    20101 tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca aaattgtcag
    20161 ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat gtgttgaaaa
    20221 ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca ttataataag
    20281 ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac
    20341 agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct tgtggcaatg
    20401 gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt tccgttgatt
    20461 ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga
    20521 aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg ttagatgggc
    20581 ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt gcgttaggac
    20641 agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat gaaacagaca
    20701 aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc gagacccttg
    20761 ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa gatttggaga
    20821 atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat acaggatttt
    20881 tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac ggtggctatg
    20941 tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca ggtgctgtag
    21001 aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga atgcaagaca
    21061 taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact gcagcaatca
    21121 ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat ggtgtagcca
    21181 aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga aaaggatggg
    21241 tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat tatgtaacga
    21301 atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca aaccaacaag
    21361 ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt gaacaatatg
    21421 aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca ggtgcagaaa
    21481 acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca gatgaagcag
    21541 taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa ccgtccattt
    21601 ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg gaaaattatc
    21661 ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta gagggggcat
    21721 tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc tttgttcact
    21781 tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc
    21841 catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat tggtaggatt
    21901 ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga ttgaaggcac
    21961 tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc ttttagacgc
    22021 ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt
    22081 catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg cttatcttct
    22141 catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat ttactaatta
    22201 tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta aaaacttttt
    22261 tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt cgtggaccgt
    22321 gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca cagcggttgt
    22381 tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct tattaccatg
    22441 ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg atagcgtagg
    22501 tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag atatcagtgc
    22561 aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag tgcagggctg
    22621 gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga tttcagaaga
    22681 cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt caaacatcac
    22741 gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata cggggaattt
    22801 caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg taggcggagg
    22861 agctggttct actgatattc ttatttcctg gatctataaa cttacgactg gaacttctcc
    22921 gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc
    22981 attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat gaaaaaattt
    23041 cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt ttttctttta
    23101 atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc cttcaagtca
    23161 ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg aaactttact
    23221 cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat catttctgtc
    23281 ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta tagtcgctat
    23341 cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat ggtaccgact
    23401 atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct cgataagtac
    23461 tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg gctgatgaaa
    23521 ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga tggggcaggg
    23581 catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct cgccgttcaa
    23641 gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt cctgcttcga
    23701 acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga tgtgacaaac
    23761 caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat ttgtctatta
    23821 ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac aaaaggatag
    23881 gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg tacgccaact
    23941 caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat atttgtattt
    24001 ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat ccaagcgatt
    24061 ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt
    24121 tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat agctgtgacc
    24181 cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca tggaactttt
    24241 attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg tatacgccgc
    24301 tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc cgaagatttc
    24361 caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat tttatcttat
    24421 agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt cggggcttcc
    24481 tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt caaagagagt
    24541 gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt tgtagggttt
    24601 atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt cttgctgctt
    24661 ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca agtttaagcg
    24721 atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga aaaatttctg
    24781 aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa tagggtagtt
    24841 tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt ttcacgttct
    24901 ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata cggagatacg
    24961 gacactcctc cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa agagattaca
    25021 agtaacgctt ctttggcact aggagaagcc tatatggatc atcgaatcga gatcgaggga
    25081 agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt acgaggaaaa
    25141 gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa caaagaagat
    25201 atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga tccaactttg
    25261 acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc acaaatcaat
    25321 aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt gcttgatatc
    25381 ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt caaggcaact
    25441 gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa agaagaacat
    25501 ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg cgaaacgttt
    25561 gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat
    25621 ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca tggaatcagc
    25681 cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc tggcggttat
    25741 gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca actgattgat
    25801 ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa gaattttcat
    25861 caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat gtgggatcta
    25921 tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg
    25981 cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag gcaaaagtac
    26041 tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt gaactttttt
    26101 gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt ttcataataa
    26161 aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta tacttgtaat
    26221 agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta tttatactag
    26281 tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag gcacatctgg
    26341 gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct caacaaaagc
    26401 aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc agtcagatat
    26461 caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag atactgcaat
    26521 tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag aaggtgtcga
    26581 tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg agacagagaa
    26641 tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga
    26701 tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag
    26761 cggtacggca acagattggg atttggataa agatttaggt actacttatt gggaagtcaa
    26821 agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag gcgaagtact
    26881 gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt tttcttttag
    26941 tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag tttagaaacg
    27001 acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt ccactgcatg
    27061 actacccagg aattagatga actggctcat tcagaagcag gagcatttat cactaagagc
    27121 tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt tcctttaggg
    27181 agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga gtatgctctt
    27241 gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat tgccgggatg
    27301 agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt taatgggatc
    27361 actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc ttacgatttt
    27421 gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc gctaggaatc
    27481 aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat cttgaatcag
    27541 tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta tatcgataca
    27601 gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg cggagagtat
    27661 atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa accagaaatc
    27721 caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca tctgctatgt
    27781 ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga aattttttct
    27841 agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc aattgacgaa
    27901 tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga ttgatcttcg
    27961 tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc attgtatgtt
    28021 agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat gatgcaacag
    28081 aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt acggatatac
    28141 agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg
    28201 agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg gctggtatcc
    28261 catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact tcaggatctc
    28321 gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa gcagaagcga
    28381 taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa aatattacag
    28441 acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca ccgggcggat
    28501 caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct ggtgctagtg
    28561 atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg ctgaaacctt
    28621 cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga gctgctattg
    28681 atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg atgagaagta
    28741 tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa
    28801 aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt agtgaagagg
    28861 cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat gatttgacag
    28921 agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg
    28981 cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct gtcagaaaag
    29041 aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc tctgccgcta
    29101 cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa aacctacatc
    29161 aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa atcagcgaag
    29221 atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt tctgaagtag
    29281 aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca ccttatacac
    29341 agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg acaaaagaaa
    29401 acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg ctatttcaaa
    29461 tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg aaatagcaga
    29521 aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta gtgccttttt
    29581 tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct ggagtaatgt
    29641 agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg catcttatta
    29701 taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg tggctaaacg
    29761 gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca cttctgtttc
    29821 aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa
    29881 attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac gaaaaaaaga
    29941 tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg gcgaacccaa
    30001 tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat atgacaatgc
    30061 cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta ttctaaatga
    30121 ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa aaatactgga
    30181 gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa atgatgctct
    30241 gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga caaagaaaat
    30301 atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc
    30361 ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac ttcacttcct
    30421 ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt tcttttagga
    30481 aatctggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc tctgaatgta
    30541 tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct gctgtttctg
    30601 ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat ctgctttacg
    30661 attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg cgtttcttca
    30721 ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt gaaatatatc
    30781 gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc tatggatcta
    30841 gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca aaaagcaaag
    30901 atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc actgatttat
    30961 tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat
    31021 cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct attttttgtt
    31081 tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa tcgaaaatat
    31141 gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc tcgttatacc
    31201 aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct gatgaccaag
    31261 gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa tggcgatgct
    31321 atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca tttgttggac
    31381 attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact gaaatcccta
    31441 caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg cctgcctctg
    31501 gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg gaagaagctt
    31561 tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag caaatggagc
    31621 gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga cgttcagaaa
    31681 atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa atagctagtc
    31741 aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat gaatatttga
    31801 ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa atatgtacta
    31861 tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac tgtcgcgagg
    31921 attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg ctacgcttaa
    31981 aaatgtgatc gaattatttc aaaaaaagca ccatccaatc gctgtgatcc aaaatcccca
    32041 agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca aagaaaatag
    32101 ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac tggattcacc
    32161 tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg tgtggaaaca
    32221 gttggcagga gttgattcga aatttttgat accagatact agtcgctttc gcatctggct
    32281 gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta
    32341 tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg cagacggata
    32401 tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag ggttccctag
    32461 ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac gcgtgaaaca
    32521 ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc ttgaagcagg
    32581 agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat
    32641 gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag tgttgaaaaa
    32701 gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc caaaagattg
    32761 gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat tagataatgt
    32821 aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca aggaaagaag
    32881 aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct tcgcaaggct
    32941 ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac gatattgaaa
    33001 gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct gcattagtct
    33061 caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca actggtcctt
    33121 atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac
    33181 cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat atccgtatcg
    33241 atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga ttcacttatc
    33301 aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa ataatcatca
    33361 attgactata aaatcatctg agcaaagata aagacattaa cactactgaa aaaaacaatc
    33421 atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag aaagaatttc
    33481 cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata gctgctttct
    33541 gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc ggcttatcct
    33601 acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc tgcagcttct
    33661 tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa acgtttcaac
    33721 tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga tgaatctcca
    33781 cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat aggacggact
    33841 accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa
    33901 tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt atgttgagca
    33961 tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt attgtcagaa
    34021 acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta
    34081 atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc agaaccagaa
    34141 ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat tgaacaagtg
    34201 aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta
    34261 taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg ttagcaaaat
    34321 tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct tcatcgcacc
    34381 agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag atataattgc
    34441 tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag caacaaattc
    34501 atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt tccatgggat
    34561 tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga aagtgatgcc
    34621 gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac cgataagcat
    34681 agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca acatttggaa
    34741 aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt ctaatacata
    34801 gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa gggcagtgat
    34861 cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag aaactgtaga
    34921 catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta attccttttt
    34981 tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat tataagattc
    35041 caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca taaataatgg
    35101 aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa ttggcatcaa
    35161 tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa caaagtcaac
    35221 accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag aaacaaccgc
    35281 tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa cgaacatcac
    35341 aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag caagccctaa
    35401 tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc tattccaaat
    35461 tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa ttttcattac
    35521 ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa aataaaaggt
    35581 ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga ttaagctaat
    35641 agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga ttttcggaag
    35701 ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt tcatcatttt
    35761 tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt ttatcataag
    35821 caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag acttcattga
    35881 tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt attgtttcaa
    35941 gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat
    36001 ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta atgatgactt
    36061 catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt tgcgcttgaa
    36121 tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga cgtaaaccga
    36181 cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat ccttttttga
    36241 agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct tcttggacaa
    36301 cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta ggagattgat
    36361 aaacagattc tttaccagta attttttcaa taatacgttt gattacagga aatgtctcaa
    36421 catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag gaatcgatca
    36481 tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc ggatgtttca
    36541 acggaacatt ccatactggg aaagtttcga acttggagta accagctgca tgtccttttt
    36601 ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct gggccaggag
    36661 ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca aaaccatctt
    36721 ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt ttatagacct
    36781 tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt ccactatagc
    36841 gagtgataac gacactgttg attaataagc cataaccacg taattcatcg attaaccgca
    36901 agatatccat atcataagta atcccataat caccacggat cttattacgt tcaatatctc
    36961 cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc aatttgattt
    37021 tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca actagcttcc
    37081 caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata tacgctgact
    37141 gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc cctccttaag
    37201 tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga aattcctcaa
    37261 aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg gtatgatagt
    37321 ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc atataaacac
    37381 tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga attggaacaa
    37441 ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca tcagcgcatt
    37501 catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc atcggattcg
    37561 attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac caaattttgg
    37621 tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt acagaaaatg
    37681 gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa
    37741 agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga atgcggatca
    37801 ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct
    37861 ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag aagatatccc
    37921 tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga
    37981 aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt tacaggaatt
    38041 tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca aaatcggcac
    38101 ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt
    38161 gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg actatgacaa
    38221 aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg aacgtttaga
    38281 tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata aaggattctt
    38341 gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc aagtactatc
    38401 taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc agcagcaaaa
    38461 agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg tagaaaaaga
    38521 acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag caatccatga
    38581 attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg aaggaggttc
    38641 cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc
    38701 atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat gacacagaag
    38761 aaatccctat gctagaggaa acaaaaatac acgaaaaaga aaaagtaact ccgacgaata
    38821 cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg tatttagaag
    38881 gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa gcagaattag
    38941 cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta ttagaatcat
    39001 atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca agcctgatcg
    39061 aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt
    39121 cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg aatggacaaa
    39181 acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa gtggaatcga
    39241 tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc
    39301 tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag atcacgatat
    39361 tcaatcacaa attgaatcaa ttattagccc atgcaagaac acttggtttt accgttaatc
    39421 agacattact agacaatacg aagccacttc gtttgaatct tcagagcgaa tttgaattga
    39481 cgaatgaagt attggatgat atttttatcg tggcaatgaa aaatccatcc tacgatatgg
    39541 aaaagatcaa cgaaactact tatcaagtac gattagataa aggacagtct ttaacgataa
    39601 caacaaacga aaaagatcaa acagtcttgt cgatctcatc tggagactac ccacgatctg
    39661 tcattgattt ctttatcaat tacgaattct tggtcccatt aatggtgaga aagtcctgat
    39721 tggataagtt ggattaccag atgtcattca gataattctg ttccaaagga cttgcggcag
    39781 ttatccagaa taattgtcac aagtcttttc tgtatgtact aaatggacgt gaataacttg
    39841 ctgatgaagg agggatttca ttaggaatgt ccacaaataa aatagcacag cttagcttat
    39901 tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt tattccgaat ttccagccaa
    39961 tgactgctat ttttttattt atagtacttt acttaagctt aaaagatgcg ttaattgtca
    40021 tgagtctttc tattgcgatc agcagttttt attttggtgt tggaccatgg gtcatcggcc
    40081 aatggatcag ctatactgtt gttctgagtt tgttttcaat cgtctgcttg agaaaaacag
    40141 tgcaaaggaa tctatggtta aagggagtct gtttttttct ggcgggcatg gtttacggaa
    40201 taggtatgac cgtttttgat acgctgttat atcgtttgcc tcaaccttgg atctattacc
    40261 ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt
    40321 ttctgccagt cgtcaaacgt ttttacaatc attcaggagg aaaaaatgaa aaaaataatt
    40381 tatagtttgc ttgcaatgag tgtcagtata ctcattttga caagttgcag tacaacctat
    40441 acacaatcgg cttccaacca aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg
    40501 ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat
    40561 ttactgagta ttttgaaacg aaattttgaa gtcaaagaag ataacggctt catcacttcg
    40621 cttgaagggc actcacagga tgagaaaaat caactctatt ggatgtttac tgtggacggg
    40681 aaaagtgcta ctactggtgc caaagagatc aaattgaatg atggacaatc ggtcattttc
    40741 aatttgtcta aactataagt ttcataaaaa actgttaaag ggcgaatatt gccataattc
    40801 agtgagttca gttgatgaaa gaaggaaaat aacatggaaa attttaaaaa aagcggaatg
    40861 ttttccgtaa ttgctgctct aggggctaat attctagttg ctatcagcaa atttgtcggc
    40921 tatgcaatca gtggcagtgc cgccatgcta aacgaaagca tccacagtat cgttgattgc
    40981 agcaatcaga ttttgctgtt attcggtgat aaacaagcca ataaaggaca gagcgagttg
    41041 catcaatttg gtgaaggacg agcaaaatac tttttcagta caatcgtcgc aatgatgctt
    41101 ttcttcggcg gtggggcact tggggtgatg gaagcttttg aaaagttgac gcatccatcc
    41161 catgaagtgg gtaatccgat cattgttata gcaatactgt tatttggttt ggtaatcgaa
    41221 ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat tgaatactga aaaactttcc
    41281 acgatgcgct ttttgcgcga aagtcgacac agtgaaatac tgattatttt tacggaagac
    41341 ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa caatattgac ttatgcaacc
    41401 gggattgctg catttgatgc gatcagcgga ttgctgatcg gtttcttgct gatgggagcc
    41461 gccattttcc tagcaaaaga attttatagt ctgatcatcg gtgaaagcgt gacagcaagc
    41521 gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga tcagtcggct gatcgatata
    41581 aaaacagttc atttaagtcc aacggagatt ttagttgcag caaaagcaga tatcgttggt
    41641 aacaaagaga atgaagctta ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct
    41701 cttcctgaca agaagatgta tatatacatt gaaacagata aatatgaccc aaattattct
    41761 cgtaaataaa acgtttattc atgaattgaa gcaaaaatga gaaaaatata atcttgtttt
    41821 taaataaaac ggctacaatt tagcgaattt atgttacaat gagtccgctg tttatttgtt
    41881 gtaattaaag gagatatttt aatgaacaaa aagaaaagtg cgccatggct tcaagctttg
    41941 catgcagtga tgccactttg tataagctat ataccagtag gattagcttg cggagtattg
    42001 ttgcaaaaag ttggatttga tcctctctta tcaggactgt tatcgattct ggtcttttcc
    42061 ggtggcgcgc agtttttagt tgcatctatg cttacgacgc aagcatcatt tgcaacgaca
    42121 ttattgatgg ttttcttttt agaattacgc tatacattat taggagcaag tcttgccggt
    42181 tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt ctcaatcact gaatgatgaa
    42241 aactatgcgg tgaactactt aaagttttca actgaccctt cctggaataa aagaaaggct
    42301 ttatatgtca attggttctc aatgactgca tgggccggta gcaatatgat cggtaatttt
    42361 ttcggctcgg tgattcgagt agatgctgac cttgtccatt ttgctttgac ggccatgttc
    42421 attttcatgt ttatcatgca gatgaaaagc gcactactga tctttacagg tttattctca
    42481 ggcgtattag gggtcgtatt catggtatta ttccaaaata ctttcggatt gattgcagca
    42541 acagtcattg cttcatttgc cggatttatg ctggagaagg cattcaaaaa agaaaaagct
    42601 aagaaactaa gagaccgcgg taaagatgtc aatgagccgc tcttcaactt cccggaacag
    42661 gaggccaatc accatgacta aactatattt gctactttta gttgcttgtc tgttcatcgt
    42721 ggcatatatc ccgcgcttgt tcccgatgct ttattttact catcgaaaag tgccatcctg
    42781 gttcagtgac tggatgaaat atgtaccggt ggcattattt gcggcgttag cttttaaaga
    42841 tgttttcatt actcatgaac atctggatat tgcttggaat atcaaaatag ctgcaatgat
    42901 tctagtagca ggcgtggctt acaaaacacg ttcgatggcg ttgtcagttt tgacaggact
    42961 tgcgtctgtc tttttgctgt ctatgcttta aactgtttta ttttattaaa aacagagatt
    43021 gcgacataag ttgttcaaac tgatctatcc gaactgcatt tgcaacagtc aattctggat
    43081 gcaagtttat tcgtagggat aaaaaagatt agtgttggct tatgatgcaa gctctttttt
    43141 attttaggaa ttagagaggg atttgtcctt ctatgatttt ttgctataat agggaagatg
    43201 tttattttat gaaagaatag ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac
    43261 attttggcta acctttgcta atcttctatg taaagtttta ggggtagtct atttgattcc
    43321 ctggctaagt atgatgggga acaatcaaga tggtatgctt gctacgacat tgtataatgt
    43381 tggctatctt ccttatggat tgttcctgat gcttggtaca gtcggatttc caaatgcgat
    43441 tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac gctgcttgtc ggctgatttt
    43501 tagaagtacg atcaatatca tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt
    43561 atttgctcct ttactagccg gaatcagtcc gattgctaac gttaataatg ggatactagc
    43621 tatcagaagt ttatgcccat ctttgattgc tatcccaatt cttagtgcaa tgagaggata
    43681 tttccaagga aaaaatgatc tgcggcctta tggaacttct ttgattatcg aacaggctgt
    43741 gcgagtgatc gttatcttgg ctggtactta ttacttgcgc gtattgactg acggaaccat
    43801 attagaagct gtattgatca gtacagtcgc ttctttcttc ggtgggttag ctgcgattat
    43861 ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc
    43921 cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc tctatcattc gggaaacact
    43981 accgtttatt tttgtcggct cagtcattac tatcgttcaa atgatcgacc aagtaacgat
    44041 gaaaccactg cttcactttt tccgtccaga gatcgcagat cagcaactgg aattcttatt
    44101 cagccgtgct tctgtcaatc caaataaatt gacattgatc ctgatctcca tggtaggaac
    44161 agtagctata agcagcttgc caatattaag cacgatgaag aaaaaagatc ggatacaaat
    44221 cgaaaaaaca gtaggagata gtttttcgat tgcgttattg attcttcttc cttcattgac
    44281 tggaatgtct ttattagctg gaccactata cacattgttt tttggctatg atcctgaaag
    44341 tgtcggatat ttccaaatgg ctttgctagc ttctttgttt ttctctttat tcacgatcct
    44401 atctacgatg cttcaatctt tgaatcacca tcgttttgcg atccgattaa ctgtagaagc
    44461 aattatcctg aaagtcatct tccaagtaat cggattagga ttagttggcg gatacgggat
    44521 gagcttatca aatggcttag cattcggtat tgtctttgtt cgcggctatc actttatgtg
    44581 caaagaatac cgaatagcac ctttagcaaa gatcagcaat tttttcttaa aaactttccg
    44641 ctccaccctg attatgcttg cagtatgttt tgccgttttc tttttgctga atcagcgatt
    44701 gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg
    44761 cttagtattt ttattttctc aattcggaaa aaatgggttt cgaatgttga aaaatgttaa
    44821 acatcatagc agaagcaaag aataggttaa ctaatcgttt tttgctgtca cttgaaaaac
    44881 ggcaactcgt atgattacgc ttgtcgtttt tctattttcc aattttagtt tcatatctaa
    44941 tcttacttat gatatagtta taaggtctgg aaaaattctt tgaaacgagg aaattaaaca
    45001 catgaaactt gcagtagtta cagatagttc ggcatatctg ccggaacgta tcaagaacca
    45061 tcccgactta ttcgttattc caatcccttt gattatggat gggaaaattt ataacgaagg
    45121 aatcgatatc gaagcggatg aatattatgg acttttaaaa aacagtaaag attttcctac
    45181 tacttcacaa cctgtcgtag gggaagtttt gggtttatat gaggaattga aagagaaagg
    45241 gtatgacacg attttaagta tccatctttc ttcagggatt tctggctttg tcaacacatt
    45301 atttgcgatg aaagatgaca tcagcggagt aacggtcatt ccttatgact ctaagatcac
    45361 tagtatgccg atgggacaca tggtagaagc tgccttagac ttaaatgatg aaggtcagtc
    45421 ccttgatgaa atacttgcac atatcgatcg aatccgagat aatacctacg cttatctgat
    45481 tgtagatgat ttgaacaatt tagtacgagg aggacgtttg acaaatggtg cagcgctgat
    45541 tggcagctta ttaaaaatca aaccgatcct gacattttct gaagggaaaa ttgttttatt
    45601 cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct gaagaaatta ttggtagaag
    45661 aaataacgaa atacaaccgc cagtcaaact ttacgtgata catgcaaata atttagaagt
    45721 agcgattgcg gagaaagaga aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg
    45781 tcatttcgga ccagtcattg gtacacattt aggtgaaaaa gccatcggca ttgccatttc
    45841 agcgcaataa ttccttttat tcaaaaaaca taccaaaaat attccaacat catcttggac
    45901 tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat
    45961 gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat atagtttagt
    46021 aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg agttaaaata
    46081 atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa
    46141 gttgatggaa gaaatgcatt aaatacggat gcaaaagggc tttcactggg ccttgctcag
    46201 tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa
    46261 tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct
    46321 gcacaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa
    46381 aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc
    46441 aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga
    46501 gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa
    46561 tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc
    46621 gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa
    46681 gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga
    46741 aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag
    46801 cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca
    46861 aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag
    46921 aattactaga agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga
    46981 accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga
    47041 tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag
    47101 cgattgaaaa aggatattta ttagagattc aagagccgac tgtcatccga gatgcctctg
    47161 ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa
    47221 tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa
    47281 atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc
    47341 ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac
    47401 ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca
    47461 tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac
    47521 aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg
    47581 aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca
    47641 tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagga agaaccatta
    47701 gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag
    47761 atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa
    47821 caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag
    47881 gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca
    47941 tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga
    48001 aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa
    48061 cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc
    48121 aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag
    48181 gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga
    48241 acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa
    48301 tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt
    48361 ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag
    48421 aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa
    48481 aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag
    48541 cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc
    48601 agccaaaagc gttgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt
    48661 tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag
    48721 aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat
    48781 ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa
    48841 cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta
    48901 aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga
    48961 acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag
    49021 caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga
    49081 tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg
    49141 ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg aagattttga
    49201 tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa
    49261 ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta
    49321 tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc
    49381 gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt
    49441 ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt
    49501 caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc
    49561 tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca
    49621 aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga
    49681 tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg
    49741 gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga
    49801 aaaagaaggg caattagatg tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc
    49861 attggatggt atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt
    49921 ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat
    49981 gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac
    50041 gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa
    50101 aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac
    50161 gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac
    50221 acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga
    50281 atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa
    50341 taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt
    50401 ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact
    50461 tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc
    50521 ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa
    50581 aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga ggaagttttt
    50641 cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg atatatttag
    50701 gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga ggtctgacag
    50761 gtacttggac atgtttgata tgcataccga ttgatgtgtc accgatatcg ataccggctt
    50821 gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca gcaacagaac
    50881 aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt tttgcttctg
    50941 ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg acagctagcg
    51001 caatttcttt aggatccaac aactctttta acgtacggat gatccattga ccaacttccg
    51061 cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa
    51121 aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt tcagtcaatt
    51181 ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt caattaatat
    51241 tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag tcccattcca
    51301 atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca atcatataaa
    51361 atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa aagccctaat
    51421 ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg taacccgtgg
    51481 atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat caatagacca
    51541 ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc ggtatagatc
    51601 ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa aattgttaat
    51661 gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt agttttcatt
    51721 tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat cgttcatcag
    51781 cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg ctttggtaaa
    51841 atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac cggccccgtt
    51901 tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa ttgaatagag
    51961 aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa tgttttttgt
    52021 tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag agggaagagc
    52081 ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa caagtggaaa
    52141 aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag tttctttgaa
    52201 tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga agtctcgaac
    52261 aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta atgaagtcat
    52321 tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt ccagcataaa
    52381 tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga atagaccaaa
    52441 attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat cagaaccgcc
    52501 aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa cgaaattttc
    52561 aaaaaagaaa aacagccaat tggcatattt ttatctcatc caatttaaaa aaacaaaaag
    52621 aaaagaaaga ccaaaatgga tctttctttc cttaaatagt taggactttt gtgattttaa
    52681 ttctttctcg atttgaagcc gttgcacttt cattgtagct gtccgcggga ttgcatcaaa
    52741 atcacggatg atagggacgt tcaagcgagg aagttcatgg acttgctccc accatcgatt
    52801 ccaatccatt tctttatcag gagctaaagc aataatcggt tgtggagaat gatttttatc
    52861 ccttacaatc actacttctg ccaaaaaatc taacgaatct aataaaaagt cttcaatagc
    52921 taatgtacta ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa ataggtggcc
    52981 atgcttatcc ataaaaccgt aatctccgct atcccaccaa tcgccataaa cattttcctg
    53041 gaaccgtgca tcttccttgt aataagtcaa agcccgtcct ttagacagta attgtatatg
    53101 accatctgtg ttttcaggta ggacattccc aactgaatct gtaatccgag cgcttgtcat
    53161 gtcttccagt ccaacgccca tatctcttgc atcacttgtt ttcaaactct ctagtgtatg
    53221 agccttaaga atcattggtc cgcattcact ttggccatat acttgaagaa ataccgcatc
    53281 ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg ttgtttatag catcaaaagt
    53341 ggagtgatag tatcggatac ccgaaaaagc ttctggatgc tccttagcag taaaacgcca
    53401 ctgaacaaag ttattcggat gggtctctag tgcaattggc cggtgagctt ccaacatatg
    53461 aacaactttt gaactttgtg cgtttgctaa aggcatcata ggaaatccca tggacatcaa
    53521 agaagagata ccaatattga atctggaatg cactggagag atgtgaaaag cgattaattt
    53581 cttttccgaa atttttgtga aaactgtttt ttgccatttc gtccgccatc ccatagaatg
    53641 agcagaatga caaataagtt tagggatacc agttgtacca gaagtatggg tcatataaga
    53701 aatttcattt ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt ccattaaata
    53761 aagaatagga a
//
LOCUS       DAZUMO010000008        51604 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000008 DAZUMO010000000
VERSION     DAZUMO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.008"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG8ER_000497"
     CDS             25..696
                     /locus_tag="ACG8ER_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5049109.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG8ER_000498"
     CDS             755..1195
                     /locus_tag="ACG8ER_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049110.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG8ER_000499"
     CDS             1207..1875
                     /locus_tag="ACG8ER_000499"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5049111.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACG8ER_000500"
     CDS             1939..2562
                     /locus_tag="ACG8ER_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS5049112.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACG8ER_000501"
     CDS             2618..3004
                     /locus_tag="ACG8ER_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS5049113.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACG8ER_000502"
     CDS             3217..4608
                     /locus_tag="ACG8ER_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS5049114.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="ACG8ER_000503"
     CDS             5219..5587
                     /locus_tag="ACG8ER_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS5049115.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="ACG8ER_000504"
     CDS             5767..6708
                     /locus_tag="ACG8ER_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS5049116.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="ACG8ER_000505"
     CDS             6777..7508
                     /locus_tag="ACG8ER_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5049117.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="ACG8ER_000506"
     CDS             7652..9832
                     /locus_tag="ACG8ER_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS5049118.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="ACG8ER_000507"
     CDS             9852..10334
                     /locus_tag="ACG8ER_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS5049119.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="ACG8ER_000508"
     CDS             10350..11111
                     /locus_tag="ACG8ER_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5049120.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /gene="murC"
                     /locus_tag="ACG8ER_000509"
     CDS             11242..12576
                     /gene="murC"
                     /locus_tag="ACG8ER_000509"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS5049121.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="ACG8ER_000510"
     CDS             12883..13368
                     /locus_tag="ACG8ER_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS5049122.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="ACG8ER_000511"
     CDS             13361..14044
                     /locus_tag="ACG8ER_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS5049123.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="ACG8ER_000512"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="ACG8ER_000512"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS5049124.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="ACG8ER_000513"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="ACG8ER_000513"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS5049125.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /gene="coaE"
                     /locus_tag="ACG8ER_000514"
     CDS             17660..18289
                     /gene="coaE"
                     /locus_tag="ACG8ER_000514"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS5049126.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="ACG8ER_000515"
     CDS             18341..18673
                     /locus_tag="ACG8ER_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5049127.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG8ER_000516"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG8ER_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS5049128.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="ACG8ER_000517"
     CDS             19499..20872
                     /locus_tag="ACG8ER_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5049129.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG8ER_000518"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG8ER_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS5049130.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="ACG8ER_000519"
     CDS             21927..22367
                     /locus_tag="ACG8ER_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS5049131.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="ACG8ER_000520"
     CDS             22511..23212
                     /locus_tag="ACG8ER_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS5049132.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="ACG8ER_000521"
     CDS             23209..24330
                     /locus_tag="ACG8ER_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS5049133.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="ACG8ER_000522"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="ACG8ER_000522"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS5049134.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="ACG8ER_000523"
     CDS             25709..26617
                     /locus_tag="ACG8ER_000523"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5049135.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="ACG8ER_000524"
     CDS             26652..26924
                     /locus_tag="ACG8ER_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS5049136.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="ACG8ER_000525"
     CDS             26935..27981
                     /locus_tag="ACG8ER_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS5049137.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28232..30841
                     /gene="clpB"
                     /locus_tag="ACG8ER_000526"
     CDS             28232..30841
                     /gene="clpB"
                     /locus_tag="ACG8ER_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS5049138.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30992..31678)
                     /locus_tag="ACG8ER_000527"
     CDS             complement(30992..31678)
                     /locus_tag="ACG8ER_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049139.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31656..32150)
                     /locus_tag="ACG8ER_000528"
     CDS             complement(31656..32150)
                     /locus_tag="ACG8ER_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5049140.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32170..33390)
                     /locus_tag="ACG8ER_000529"
     CDS             complement(32170..33390)
                     /locus_tag="ACG8ER_000529"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5049141.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33537..35372
                     /gene="lepA"
                     /locus_tag="ACG8ER_000530"
     CDS             33537..35372
                     /gene="lepA"
                     /locus_tag="ACG8ER_000530"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS5049142.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35568..36479
                     /locus_tag="ACG8ER_000531"
     CDS             35568..36479
                     /locus_tag="ACG8ER_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049143.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            37475..37726
                     /locus_tag="ACG8ER_000532"
     CDS             37475..37726
                     /locus_tag="ACG8ER_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049144.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            37839..38081
                     /locus_tag="ACG8ER_000533"
                     /pseudo
     CDS             37839..38081
                     /locus_tag="ACG8ER_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38171..38410
                     /locus_tag="ACG8ER_000534"
     CDS             38171..38410
                     /locus_tag="ACG8ER_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049145.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            38557..42840
                     /locus_tag="ACG8ER_000535"
     CDS             38557..42840
                     /locus_tag="ACG8ER_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFS5049146.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            42939..43304
                     /locus_tag="ACG8ER_000536"
     CDS             42939..43304
                     /locus_tag="ACG8ER_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049147.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            43306..43758
                     /locus_tag="ACG8ER_000537"
     CDS             43306..43758
                     /locus_tag="ACG8ER_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049148.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            44207..45682
                     /locus_tag="ACG8ER_000538"
     CDS             44207..45682
                     /locus_tag="ACG8ER_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS5049149.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            45705..46016
                     /locus_tag="ACG8ER_000539"
     CDS             45705..46016
                     /locus_tag="ACG8ER_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049150.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            46377..47633
                     /locus_tag="ACG8ER_000540"
     CDS             46377..47633
                     /locus_tag="ACG8ER_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5049151.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            47630..48175
                     /locus_tag="ACG8ER_000541"
     CDS             47630..48175
                     /locus_tag="ACG8ER_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049152.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            48176..48676
                     /locus_tag="ACG8ER_000542"
     CDS             48176..48676
                     /locus_tag="ACG8ER_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049153.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            49035..49313
                     /locus_tag="ACG8ER_000543"
     CDS             49035..49313
                     /locus_tag="ACG8ER_000543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049154.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            49333..49677
                     /locus_tag="ACG8ER_000544"
     CDS             49333..49677
                     /locus_tag="ACG8ER_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049155.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            49696..50607
                     /locus_tag="ACG8ER_000545"
     CDS             49696..50607
                     /locus_tag="ACG8ER_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5049156.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            50686..>51604
                     /locus_tag="ACG8ER_000546"
     CDS             50686..>51604
                     /locus_tag="ACG8ER_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5049157.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVIT"
BASE COUNT        17573 a         8597 c        11060 g        14374 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc
    28081 aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata
    28141 ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg
    28201 atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct
    28261 tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga
    28321 cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac
    28381 ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta
    28441 tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg
    28501 attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga
    28561 agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa
    28621 aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag
    28681 agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt
    28741 agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg
    28801 tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc
    28861 cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt
    28921 gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg
    28981 agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa
    29041 aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa
    29101 aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt
    29161 acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc
    29221 gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag
    29281 tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa
    29341 cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga
    29401 caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat
    29461 gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc
    29521 actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt
    29581 agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga
    29641 agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga
    29701 tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca
    29761 attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt
    29821 tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc
    29881 cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca
    29941 taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc
    30001 acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac
    30061 tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga
    30121 tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt
    30181 cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag
    30241 acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt
    30301 caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt
    30361 agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga
    30421 aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt
    30481 acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc
    30541 attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg
    30601 tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga
    30661 aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt
    30721 agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac
    30781 gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta
    30841 atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct
    30901 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
    30961 aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
    31021 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
    31081 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
    31141 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
    31201 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
    31261 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
    31321 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
    31381 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
    31441 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
    31501 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
    31561 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
    31621 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
    31681 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
    31741 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
    31801 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
    31861 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
    31921 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
    31981 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
    32041 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
    32101 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
    32161 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
    32221 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
    32281 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
    32341 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
    32401 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
    32461 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
    32521 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
    32581 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
    32641 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
    32701 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
    32761 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
    32821 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
    32881 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
    32941 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
    33001 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
    33061 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
    33121 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
    33181 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
    33241 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
    33301 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
    33361 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
    33421 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
    33481 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
    33541 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
    33601 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
    33661 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
    33721 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
    33781 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
    33841 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
    33901 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
    33961 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
    34021 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
    34081 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
    34141 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
    34201 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
    34261 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
    34321 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
    34381 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
    34441 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
    34501 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
    34561 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
    34621 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
    34681 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
    34741 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
    34801 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
    34861 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
    34921 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
    34981 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
    35041 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
    35101 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
    35161 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
    35221 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
    35281 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
    35341 tgaaaatgga tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa
    35401 taaacctttt tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc
    35461 atttggttat cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt
    35521 tagctgtcag tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt
    35581 caagtggaag ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag
    35641 ctgatttagc aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa
    35701 tccctaaaga cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag
    35761 atgaggaaga taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata
    35821 agttacttaa caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg
    35881 tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat
    35941 taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat
    36001 caaaagacga taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag
    36061 agacgcttga aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta
    36121 gatgggaaaa aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg
    36181 gtaaggtcac tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag
    36241 atgaaagagt tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga
    36301 aaattaggaa agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa
    36361 tttcaaaatg gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa
    36421 ttatccaata tgcttatata ggaaatcttt ctttagaatt tttgatttac gggatataaa
    36481 aatagtttac gtcgatactt attaagttat cgacgttttt tattatagaa aaaatttata
    36541 aaaggaggtt tcttgatatg ataagaaaca aaaagggttt gttgaaagtt gctttactgg
    36601 cagctttttt ctaagtaact ttttgacaag ctcaatggca acacaagcgg aagaagtaat
    36661 acaagaacta actgttatga ataacagcta gataaaatgg gctatgtatg ttatccaaca
    36721 atttagtaat cactagaagc ctagcgaaag ttcgcttgat aagttataga tgagtattga
    36781 tttgtagtgt ttccaaatca aaagggtaca gagccacatg aaatatgttg tcctctgcag
    36841 cgtcaggatg ggaatccata ccatggagat tcggagatct gtgagggaat acttacgtgg
    36901 ttaatatgtc agttgtgttg gacgtagtaa tggtcatagt gggtagattg actgaattct
    36961 tatatgagaa aatgaaacca acaagaagat agggttattt atacagaatt ggggatcttt
    37021 tctggaaaag taatcggata atgcgagatt cgtacggtct tttttaaatt tctctaaaaa
    37081 aaacggcatc gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata
    37141 aaaaatgatt ctatttatct ttttacatct atcgtttttt caagtagccg caaattatat
    37201 cagcattcaa caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt
    37261 gttgtgaaga taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt
    37321 aggttaggac aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag
    37381 taatttccga ttattggagt ttttttattt ataaagaaga agcgaataag ttgtgattcc
    37441 tagatttcgc gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac
    37501 acgaggacaa agtttcttag gtattgaagg cacacgtgta acggttgatg ggtggtgtga
    37561 ttgtttcgag gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa
    37621 caacgtggaa tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa
    37681 atttattttg cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta
    37741 gagatcattg ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag
    37801 gtgatagaat gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga
    37861 ttcttagaaa aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg
    37921 taggtgtcta tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag
    37981 atgcctattt acaaaatagg tatcgagtag atggttttaa gcaagacttt gcggagtggt
    38041 ttgaatggga aattgaaaac ggagaagtaa aagaagaata agagtacttg ttgtatcaaa
    38101 ataagccagt agaacaacca aaccagtagc tagtaatgac aataatactg aggaatcgga
    38161 tcattatggt atgattgata tttctcttcc agaagatctt aagccagatc ctgtaacaat
    38221 tggcaatagt gtcatgattt ttgattcatt agatgaagct atgtcatatg gttggaaaga
    38281 aattaaggat gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa
    38341 aaaagctggt attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt
    38401 tgttctgtaa ttatagtggg agggtaaaat cattgatgaa atgtgttcag ataagagcgc
    38461 cagataaaaa tagaagagac agttcacgtc gttatcactt ttggtaacgg cgttttgttt
    38521 tattatataa aattctcagg aaaaggagga attcttgtgt atgcaaaaac gaaaatgaat
    38581 tggttgaaag cactcgcatt ggtggctttt tctcaattct gtttcttggt gggaactgct
    38641 caagcacatg cagaagaaac gacggctata caacctacga tggaagccat tgcccaagtc
    38701 gcttctgaag tgacagaagt ccaaccagca gaagccgggg tcccagctgg tgaagccact
    38761 gttgaacagg cccctgtgac agaagcgcca acggtcaatt ccaaaacgac tctcgatcag
    38821 acgattgggg aaacacaaca cccagggaat agtcctggta cttcacaacc agttgaagaa
    38881 actgcctcaa ttacaacccc tgcttcagaa acgtccactc tagaaggaga aaaagctgcc
    38941 gaagcaacgc cttctgtgca gccagagtct tctgagaaac aagcggaaga aaagaaacga
    39001 atagaagaaa cagtggaaca accagcggct actgctgaca aagaaggaaa aaatgaggaa
    39061 aacgttgaaa aaacaatctc ttcaaccgca gctagtcaaa atcaaccaac gaaaccaaac
    39121 aaagaatcta cggctagtat tgaaaatcaa aagaaaaaag agcagaacat tccaaaaaca
    39181 ggtacaccta cactgttagt aacagtacca accaatcgtt cggttcgtgt tcgccgtgca
    39241 accaatgcag ctgttttagg tggtcggaaa attgattggg gcgaaaatat tggtgttact
    39301 tctagtccga acaatctaaa cttcacttta aattatatgg ttcgttttga gattaatgga
    39361 caagtggcgt tttgtattga gcctggtgtc tcagccgcag aaggtcaagt ttatacagct
    39421 actgctttga aaaactattt gaaaaattca aatgtacgga aaaaaacaag cttgatctct
    39481 tatttaggat atgtcacaaa taaagacaaa tccgatgaac aatacattgc ggcacaattt
    39541 atgatttggg aagctttggg cacaaaaatc ccaatcaaaa atatccgtgt tgattatgat
    39601 cgtcgaaaag cagaaattca acgtttgatt aataactttg gtgaaaaagc aacttttaat
    39661 aatcaaacac atacggtaaa aattggcgaa acgttgaaag taaagaataa aaatgatttt
    39721 ttgaaatatg tgaaagacat tcaaaaacca aaaggtgtca atgtatctat tcacggcaat
    39781 gaagtatgga ttacagtcac aaaagaggca ccggataaag caaaaattca attgaataaa
    39841 attaaagttc aaggtgcgcc attagcctat cgtcgtccag gagctcaaac aattggtgtg
    39901 ttacatccaa cagaacctaa tgcgtctgtt attaatttac aaattctaaa acaaggaaac
    39961 attcgtgtgt ttaaagaaga tgcggaaact ggtgtgaaag cacaaggtgc agcttcttta
    40021 gaaaatgcgg tctatggttt gtatcaagca gatgggaaaa agattaaaga aattaccttg
    40081 aaaaaaatta acggcaaagt acaggcagaa attaaagatc ttgatcctgg caaatatgtt
    40141 ttaaaagaaa tcaaagcgcc aaaaggttat gtgctttctg atgaagcaat tgaaattgtt
    40201 gttgaacctg gaaaaactgt caatgcgacc gcaaaagatc aagtgatcaa aggaaccatt
    40261 gatttagtga aagttgctaa taaagattta gtcgattcaa cgaatccaga taacaaacca
    40321 aaattagctg gcattgaaat cactttgacg tcaaaaacaa ctggcaaagt ggtgaaaacc
    40381 gttgtgacag acaatgatgg ttatgctact tttggtaaaa ataccgttgt ctttgatacg
    40441 tacattcttt ctgaaaccaa aggaaaagaa gggtataaac tatttgaacc acttgaagtc
    40501 acaatttctg aaaatgggca aactttccat tatgttttag aagataaagt aattgaacaa
    40561 cgtcttaaag tggtgaaagt ggatcaagaa acaggaaaag tgattccact tgctaataca
    40621 gaattcaaga tttttgatac tttagccaat cgttatgtca caatggagat tccaaatgat
    40681 acagaaacaa cggatatctt taaaaccaat gaaaaaggct ttttcctaac taacggtaca
    40741 cttacttatg gaaaagatcg ctatcgtttg gaagaaatca aagcccccgt aaattacgtt
    40801 ttgaatcaaa acccattagt gttctcgatt accaatgata cagaaccgat caaagtgatc
    40861 cattttgcga accgtcaagc acggaaaagt gtaaaattat tcaagtatga agaatggaac
    40921 aagaagaaaa cgccgctcgc aggtgtgaac tttaccttgt ataacggtaa aaatcaagca
    40981 attggtgaat ataccactga tcaaaacggg gaaattttag tgaaaggtct agttgccgat
    41041 gactattact tcttggaaaa tgccccatta gaaagtttcc aaccaaatac agataaacaa
    41101 gcattcacgg ttgcattcaa acaaaatgcg ccagatgatg gggaaacgtt agtggtttct
    41161 gtacgtaatt acttgattcc accaaaacta gaaacacaag cgtcaaataa agccgatggt
    41221 gaaaaagtgg ttgatccatt agaaacgatc gtcattaaag acagtgtttc ttataccaac
    41281 ttgtttgtcg gaaaagaata caccatttca ggagtattga tgaacaaggc aacgaatgcg
    41341 ccaattttac aagatggcaa agaaatcaca agtaccttgg cctttattgc agacaaagtc
    41401 aatggcacaa aagaccttga atttgtggtt aatgctagtg ttctgaaagg tcaaacgatc
    41461 gttgtttttg aaaaactttt ccgtgatggc attgaagttg cagcacatgc ggatatcaac
    41521 gatgaagccc aaagtgtgaa agtgaataat ccaaccgttc ataccacggc tgtctttgaa
    41581 gacggattga cagtcgcaga cccaacagga ttgtttacct taaacgatac cgttgaaatt
    41641 accgagattc ttccaggaaa agcatacttg gtcaaaggga tcttaatgga taaagcaacc
    41701 ggtgaaccct tgcttgtgca tggaaatacg gtagaaacga gtgttggctt tattgccaaa
    41761 gcagaaaatg aaacggtgat cgtaccattt acctttgatt tgacaggtat aaatggtcgt
    41821 gaaatcgtcg tgtttgaaag tctttttaga aatgatgaag aactagcaaa ccataaagac
    41881 atcaacgaca aagggcaaac ggtacgagta actaacccaa gtgtacgtac aacggctaca
    41941 cataaaccca ctggcttgaa agaggttaat ccattaacga aagtcaccat tacagacgct
    42001 gtgaaatacc acgatttggt cgttggaaaa acatacacag tcaacggtgt tctaatggac
    42061 aaagtaacgg aaaaaccagt gctagtagat ggaaaagaag tcacaagtac cttaacgttt
    42121 gtggcagaaa caacggatgg tacagtcaat ctagacttta ttctagacgc acgtgcgttg
    42181 cgaggcaaag aagtcgtggt ctttgaagat ctgtttagag aaggcgtatt attggcttct
    42241 cacgcagata ttaccgacaa tgaccaaacc atcaaaatca gtaatccaac cattacgacg
    42301 aaagcgacga ataaaaatgg cggaaaagag atcttggcct tgccaaatca aactgtgctt
    42361 gaatgggtca aagtagacgg tcttgcgatt ggtcaaatct atcgcttaat tgttcaaggc
    42421 tacttaacac cagacggcac accatttgaa ggcactcatg cggaaaaagt ctttgcggcc
    42481 gataaagaaa tgatggaatt cttatttgaa ttcttagtgg acggtcgcaa tcttgcagga
    42541 aaaggcttga gttttgcgga atggctccaa gcgaaaacgg aagatgagaa agaaacgttt
    42601 gaagaagtgg cgaaacacaa tgtagatcta acaaacaaag atcaaacggt tcaaattgtg
    42661 gaagcgccaa aaccaccgca accagtgaaa ccagaacaac gggttaaacc agctactgtt
    42721 gtaccaatgt tgccaaaaac aggtgaacaa acaagtagta tacttatggt tcttggatta
    42781 gtagtattag tcattgcttt aggtataaca ctttataaag tacgtaaagc caaagattag
    42841 tcatgaggaa caagcggtta ggatttttaa ccgcttgttt gattgtaatg tagaaatatt
    42901 tagaatttat tttggttttt aagaggagga aaaactttat ggcagaaaaa gcattgcgta
    42961 tgaagaaaga gtttttaggt caagtatttt tattgggttc acgggaacct aatccaccaa
    43021 aggaaattaa tggtgtgtct gggcgatatg ttcaattaca agcagagtgt tcgcgagaag
    43081 aacagcctgc tttttttgtt tatctcacag ctgaagagga taaaagatta cgattaccag
    43141 aaaaagaggt gcgtatttca tttgaagaag cttattttgt ttttcgtaaa gaacgagtga
    43201 atagtcgtga aacaaatgat atgggacaaa cagagcgtcg aacaactggc tatcaagatg
    43261 ttggtgtctt tttaactggt gtaaaaattg gggaggtttt gtaagatggg attaaaattt
    43321 ccaaataatt atgacattac agaagcatta gatgaaactt gtttaggagc cgtacgcttt
    43381 ggcgcattag aagagtctga tcgtgtattt gataatgaaa caggcgatta tacgggagaa
    43441 attcgatcac agcgagcaca attagttttt ggaaatccag atgaaccaaa gattgcacga
    43501 cgagttaaat ttccagcaga tgtagatttg actggtttta aacgacgtga tcctgtgaaa
    43561 ttaattgatc cagaatatca tgtggtatat attccagcac aagatggcaa tcgtgaatat
    43621 tcagatttag tgatttatgc gaaaggatta gagaaagtag aagctccaac aaataatcct
    43681 aatatgaaac aatcacaaga aaaaggttta gatcaaaaac aaccaagtgt tgattctaag
    43741 gaaaaagaga aaaaataagg tttttttgaa agaataggaa ggagtcttcc tattcttttt
    43801 atctaagata gaaggaaggg gagattttga attcagataa aaccaatcaa atactttgag
    43861 agattgtgct tgttatcaaa atatccaaca attgattgag tattttggag acaatccaac
    43921 gatttttgta caaacaaatg catatatttg tgcggttatt gggcagtgta tgtttttacg
    43981 tgatcttgtt cgtttttcat taccgtacca gtggaaacaa gatcatcagg aagtgatttg
    44041 gattcgtata gtacgaatat gtaatatgtg tgcacaccct tataaaaatc gattaaatct
    44101 ttttcgatta tgttttttta ctaaatgtga tattcctgta ttagttgctt tttgtcaaaa
    44161 gtttttagca caggaaaagt aaaaacagaa aggaggaatg tgacaattga aagctcattt
    44221 tttcaagtat cgtggtcggc gaattcgcta tttcaatcgt tatttatggt tttgctatta
    44281 tggtttcttt ttgtttccat ttttgattct gttaggttgg tttagttgga aagtatttta
    44341 tccacgttta agtacgtttt cgaattggca aatgtatctt attcctggaa taagtattgt
    44401 gttgtatttg ttgctggtta gtgggttaat tttagggttg atgcattggt cgcgactaaa
    44461 agaaggatat tttgcctctg tttattggcg tcagttactt gttcgaatgt taattgacaa
    44521 tcggtttgtt tatacaaaga aacaagttag tgagaaacaa acaagggaaa aaatgagatt
    44581 accaaaagtt tatttttatc aaaagaaaca agaattagtg gtttcttttc ctttagatgg
    44641 tgggcgtttt cacgaacgat ttttaaaaat gggtcatttg ttatctgaag tatttttgcg
    44701 tgatttaatt cgtgaagaac gagaaaaagc ccgaatcaat tatgtattcc tttcagattc
    44761 tggtcggatt cctattgatc aatgtatagc agaaaatgga cgtattcatt taatgaaaac
    44821 tttggattgg gtatatgatc aatcaccaaa tatgttgatt gctggtgcaa tcggtggtgg
    44881 gaaaacctat ttattgtatt ctttaattca agcttgttta agtgttggga cagtagatat
    44941 ttgcgatgga aaagcggcag atttagcagc attaggtgac gtcgatgtat tcaaaggaca
    45001 tgtgttttac aaaataaatg aagaaatgat tatttgttta cgaaatgcat taaaagaaat
    45061 gaatcaacgg tatgcctata tgaaaaccat gaaagatcct aaatatgaac cagggaaaaa
    45121 ttatgcttat tatgggttac caccgcattt tatctttttt gatgaatggg cagcttttta
    45181 tggttcgttg gatatggaag cgagaaatag agtcgataaa ttgattcagc cattagtatt
    45241 aaagggtcgt caagcaggga tgtacattat tttagcaatg caacgaccag atgcagaata
    45301 ttttccaagt ggtgttcggg ataatttaac attccgtatt tctgttggtc gattgtctcc
    45361 cattggttat ttaatgactt ttggagaaga ttttaaaaat aagccatttt acaacaaaga
    45421 tcaacctggt cgtggatatg cttttccagg atttgatgta cctgtagagt tttttgcgcc
    45481 atttgtttac aaagattatc actttatcaa agaatttgag aaatttactg aaatgattgt
    45541 ctatgatttt acagtaaaag aggatagtca agatcaaacg atgggagatg ataatcagtt
    45601 gttacaagaa caactagagc gccaaatgta tttagcaaaa gatccaaaag gaacaagttt
    45661 ttctgatgcg catattttgt aatcaactaa ggaggtgaag ttaaatggca gagagttgtt
    45721 attatagagt agctacagca atacgaatga ttaatccttc tctttcttcc agaacgtttt
    45781 atgattggtt gaatcgtatt gaacaagtaa ctgattaccg ctttttgcgc aaagaacgtg
    45841 tttttacagg aaaagttatt aatcaagtat tgctaacgaa aaaagatata gaacgattga
    45901 ctcgattgta tcattatcga gtagatttag aagaggattt aactttgtca atttatcgcg
    45961 ttttttctcc tgaaaaatat agtgaaataa caaaattaga tcatttaatt ttgtagttat
    46021 tatctatcgg gagggctcta gacgtagtct ggtagccgca ctctgcctgg cggtgcgcat
    46081 taaggctgcg aagcatgggc ctataatgcg cactcgctag cagcgtgtgc agctcacaaa
    46141 gaggtcgtgc gcttgcgcac ggcctctttg tgtaaagccc cccgatatat aatatggggg
    46201 gcagaaataa cataacacat agaaaaaagt tttataaacc cttttgtatc aagtgattat
    46261 ctaaggtcag cagttgtact acttttccca aaaaacgagg gggactagtt gatatatcgg
    46321 tgtttctaaa gggttaaaaa aaatcgattt tgttatttta taaaaggagc gagaaaatga
    46381 caccgattga gctaaaaagc tacagaaaga agttaagtgc aattcataat ataagtggag
    46441 ataaaaagcc gctatcacaa gcaagatttg cgcaattgat gggctgctca aaagattaca
    46501 ttagaaagat ggaacaaggg aaaagaaaaa tcccagagga ttttgaacaa cgaatcaagg
    46561 aacgctttcg attgtatatg aaaagcggaa tagatcttcg agcaatgatt gattatttgc
    46621 gtctttcctt ttttgggaca gactacaaaa cagtgattca acgtgtatta cgaatggatc
    46681 tgacagaatt tgaaacacgt ccaacgggct tgtatctttt tgactgtctt tctgttcgtg
    46741 ggaatatctg ggtgttctgg cattcaaaac gaactgtaac agaaaatgta cttgtgcaat
    46801 tttcaggaca aggttgccgg gaatatgagg aagtcatgcg tgaacaacaa acagattggc
    46861 gaacatggct atattccatt tggaaaaatc aaggaaagtt ggaagctggt tatacacgtg
    46921 tgcaatgtag ccgattcgat attgcgttag atgaactttg gggattggaa acggaagaac
    46981 atttcgatct ctttgaatta ctagaaaaac aaaaagcagg tttattagaa atggacttta
    47041 agagattcca gaatattggc ggttgttttt tagatgatgg ctattttcga agtgaaggat
    47101 tatctttgta ttttggatcg cgaaagagtc ctctcttttt taatttttat gagaaacgtt
    47161 ttgaaattgc gaaccgagaa aaaatttcag aattagaagc attaacgaaa tatggtattt
    47221 ataatcgata tgagttacgg ttagccaatg agaaagcaac acaagcagta gaagcattta
    47281 ttttaggtga ttcaccgaaa cgtttaggag agattggcgt tggtttaatt aatgcaaaac
    47341 ttattgtttg ggatagactg gaaagtgatg cacggattgt tgataaacga tgggcaaaat
    47401 tatttcgttc gatggaagaa ttggatttta cagtaaagcc agaaaaattc tcattagatc
    47461 gttcagaacg ctggtttttg aaacaagtag ctccgacatt aaagttgtta caagaagcag
    47521 atcgtatttt aggaagagac tttgtacaag aagggattga taatgccgaa ctaaatgacg
    47581 agcaacaaaa ctatctagaa tggatgggaa cgtcaggagg gaaaattgca tgaatgcata
    47641 tacgacaaca gaatggctgc gcctgttgga tttaaaggct gcaatcgaag cattaaatga
    47701 aaagatggtt gatttgtctt attttcgttt tcgagttcct tatattgaac aagcggtaaa
    47761 agctggacgt tatcaggaaa aagagaactg gcaagaaatt gcacgcttat tagaagttcg
    47821 taagggatac gaacaagaac tcgaagagtt ggaatttagc cgaaggaaag gaaggctaga
    47881 attcattcgc ttttatcgat tttcattgcc aatacctgcg attttagcgg ttaaaaaagg
    47941 ctgcgataaa atgaagatat atgaaaactg tgtggcagca cttagcagcg aaaaacctct
    48001 tatggaagaa atttcattgg tgactgtcac ttgggaaatg agtcgccagc caacagaaga
    48061 acagacatat ctttctttag aagagattga gatagaatta gaggaaattg gtcgctatgc
    48121 cacttgttct acctattgtg gatcggtcat tagtattgct ggtgtgattg tctaaatgaa
    48181 aaaaattatg atttatgttg gtgcgtattg gtcacgtgat ccaacagtat tagaaaatcc
    48241 agaggcggta cactattgct tgcgccaatt attctatctg tataaagaac gattagaatc
    48301 gttgattcgt cagttaccat atactgacag acgtttagat gaacttcttc taaggtatcc
    48361 agcaatgtat aaacgacgaa aaaatagatt gttaccagaa gagtatccca ttgaaaaaca
    48421 tgaacttgaa gggcgatttg tggcttattt ctatgatgat gttcgtatgc gcttagtaga
    48481 acaacgaatg gaaatagaaa atgatcgcta ctattttatc aattattgta agaagcgtaa
    48541 gtatcaagta actgatgatt tttatgattg tattttacaa gatggggaag tgattctatc
    48601 caaaattgct ccaagttttc gtgaacttgt gccgattgat tttttgaaaa aatgtcattt
    48661 gcggattctt ccgtaaaaaa gagctataaa aggaaagaaa agtagaagcc aaatcggttt
    48721 ctgctttttt atttttttta ggaaggagaa gatggtatgt cactggaatt tttaggagag
    48781 ccaattgaaa aggaagagtt cgtttcgcat tatatggatt tgtttattca ggtgataaaa
    48841 aactttgtgc gattgatgaa ttttatccac gcgaacaaga ctatctagcc gctgaacgaa
    48901 taaaaatcag tttattatgg gaaaaattca cagatgaaaa taaccgaccg ccagattatc
    48961 gctatttatc agattgttta acgaataatg tgctttctaa agaataccta tttcacaaac
    49021 gtgagaaaaa tcaaatgtcg gttgaacatt tttgtgattg ttatattgca ttattgcgta
    49081 gtcaacagtt attaacagta gaacgatatg gtgcagagtt tggttatgtc atgcaagctg
    49141 agcgtcatag agctacaaga tgctatgaaa aagaagatca agtgattgat ggctatgaag
    49201 aattatgtat tcgaaacaat cgctatttgc aacaacgttt atatgaacaa ttgatgatgg
    49261 atttttcaaa tctctataga gggtatgaat gttcgatgat tcaagaaagg tagaagaaaa
    49321 gggggaattt ttatgtggcg attattattt ttggtggtat gtggtatggc attgttctat
    49381 gtgtatcgtg tctatctcta tccacgaatg gaacgatgga caagttggta tagagatcct
    49441 ttaaaacaac gaaaaggatt gaagcggtat cgttatgaat tacttgttga attttcggat
    49501 gggcgacaag cacagtatta tttttttgca aactatccaa aatacaacac cagtgaacta
    49561 ttgattcaat tattcaaaaa taatccatat gttgggttag aagaaaacgg acgggagttc
    49621 ttttatcgtt cagataatat tagtgcggcg tctatgaaca aaacacgaat tgcataaaaa
    49681 attggaggaa taatcatgaa aaaaacaaca ttagcagtcg taacaacact aggaatttca
    49741 ttattttcgt ttcaattagg gagttcattg gtttatgcag atgaaactgt agaaacacct
    49801 aagtcagaaa ttgtagaaac attgccaggg gaaggattac cagatgttga gacagagcta
    49861 aatggaggaa caacggaaaa accagcagaa cctgaacaat tgcagtcaga gaaacctgaa
    49921 aaagagccag tgacaccaga accagagttt cctgaatcaa ctttaccgac tgattcgatc
    49981 gaagaagaag gtgtgcatag ttcaacaaca acggattcaa gtatagtcac aactccggaa
    50041 caatcaggaa caaccagttc ttctgaaaag aatactgatt cgtcaagtac ggaacagcca
    50101 acgatgtcgt ctaccagtga agaacaaaaa gaagaaccga aacaacctgt tgaaaaatca
    50161 gaagcaattc caaaagaaaa taaacctatt gttccagcaa aagaagtcac agtatcggta
    50221 accccaagcg gtgaaattac gacgaatact tctcaaggaa tgagtgtacc gattgtcact
    50281 agtaatgtag aggaattgac ccatattcca acaccaacca ccccattaaa agtagcaggt
    50341 gatcaaacaa ttgttggtgt aaaagatggt attccattaa tccaagatag tgaagggaat
    50401 ttggtaaagg atttgtcgat tcctgtaaag aaacttccaa gtggcaatat tgaaatcaaa
    50461 acagcagatg gaaaaacaaa agtcttacca aagactggtg aagaaataca tgttgcactt
    50521 tctgttctag gaggagtgtt gacaagtatt gctggatttg ttggatataa aagaaagaga
    50581 aaaggaatag aaaatgaggg taattgaatg gactattttt cagaaaaaca agcttatcgt
    50641 tgtcaatcgt acgtactagt aaaaacatac tagaaggagt agcttatgaa aaaacgacaa
    50701 ctacggcgga aagaaaaagt ttatacagag aaagtaccga agcaaaagaa aatccattca
    50761 aaaaaagcag tgaaatggat ttggtgtttg gttttatttc tttgtttttc agggggcttg
    50821 gcattttttc aatccatgaa aacgaaggtt caagtgtcgt ttttgaaaga tgaaatccaa
    50881 caattacaaa agaaagaagt acaaagtcgg tcagaattaa cgtacacacc tgaaattgaa
    50941 tcctttatga atcgttttgt tgctttgtat atgaatacat tcgataatca tgaacaagaa
    51001 caacaacgtc aagaaacatt aaaaaaagag ttttatgcac aaaactttat tcaagaacca
    51061 ggaagtgttt caggtgttcg aaaattaact gctgcttcgt ttattggatt aaaagaaatt
    51121 gaaaccattc caactgctag ttataaagta acttatgata ttcgctcgat gaatccagag
    51181 agtaagaaag aaacaagtgt gacaatcaca caagtactca atcttccctt ttctatttca
    51241 aacaagggct gtcgtattat tgcttatcct tattttacgg cactaccaaa gaatacagaa
    51301 aaacaacgaa tgattactta tgatgacaca ttatacgaaa cggttgatgc gtcggaagcg
    51361 aaagatgttg aaggatttgt ggaagatttc ttagataagt atgcgaaaaa caatgcaaaa
    51421 gatatgtcat acatgatgaa agaacctgaa ggactcaatc aaacagctaa agttgatcaa
    51481 gtagagatga aagtattgcg ttataaagaa acgaaaaaac cttttattgt aaaagcaacg
    51541 gtcgtctttc gagatggtga tacgtcactt ttacaaaaag aacaaatgac gattgtcatt
    51601 acga
//
LOCUS       DAZUMO010000009        51261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000009 DAZUMO010000000
VERSION     DAZUMO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.009"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG8ER_000547"
     CDS             330..1175
                     /locus_tag="ACG8ER_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5049158.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG8ER_000548"
     CDS             1456..2676
                     /locus_tag="ACG8ER_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5049159.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG8ER_000549"
     CDS             2673..5393
                     /locus_tag="ACG8ER_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5049160.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACG8ER_000550"
     CDS             5377..6321
                     /locus_tag="ACG8ER_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5049161.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="ACG8ER_000551"
     CDS             complement(6390..7400)
                     /locus_tag="ACG8ER_000551"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5049162.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="ACG8ER_000552"
     CDS             complement(7580..7924)
                     /locus_tag="ACG8ER_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5049163.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="ACG8ER_000553"
     CDS             complement(7917..8351)
                     /locus_tag="ACG8ER_000553"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS5049164.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="ACG8ER_000554"
     CDS             8489..9226
                     /locus_tag="ACG8ER_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049165.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="ACG8ER_000555"
     CDS             9219..10430
                     /locus_tag="ACG8ER_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049166.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="ACG8ER_000556"
     CDS             10634..11416
                     /locus_tag="ACG8ER_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS5049167.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="ACG8ER_000557"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="ACG8ER_000557"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS5049168.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG8ER_000558"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG8ER_000558"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS5049169.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="comGA"
                     /locus_tag="ACG8ER_000559"
     CDS             14132..15061
                     /gene="comGA"
                     /locus_tag="ACG8ER_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS5049170.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTIW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /gene="comGB"
                     /locus_tag="ACG8ER_000560"
     CDS             15018..16067
                     /gene="comGB"
                     /locus_tag="ACG8ER_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS5049171.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /gene="comGC"
                     /locus_tag="ACG8ER_000561"
     CDS             16064..16342
                     /gene="comGC"
                     /locus_tag="ACG8ER_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS5049172.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /gene="comGD"
                     /locus_tag="ACG8ER_000562"
     CDS             16317..16787
                     /gene="comGD"
                     /locus_tag="ACG8ER_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS5049173.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16759..17106
                     /locus_tag="ACG8ER_000563"
     CDS             16759..17106
                     /locus_tag="ACG8ER_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049174.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17069..17494
                     /gene="comGF"
                     /locus_tag="ACG8ER_000564"
     CDS             17069..17494
                     /gene="comGF"
                     /locus_tag="ACG8ER_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS5049175.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /gene="comGG"
                     /locus_tag="ACG8ER_000565"
     CDS             17466..17825
                     /gene="comGG"
                     /locus_tag="ACG8ER_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS5049176.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="ACG8ER_000566"
     CDS             17997..19004
                     /locus_tag="ACG8ER_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5049177.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="ACG8ER_000567"
     CDS             19029..20213
                     /locus_tag="ACG8ER_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS5049178.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG8ER_000568"
     CDS             complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG8ER_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS5049179.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22338..22466)
                     /locus_tag="ACG8ER_000569"
     CDS             complement(22338..22466)
                     /locus_tag="ACG8ER_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049180.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22811..22954)
                     /locus_tag="ACG8ER_000570"
     CDS             complement(22811..22954)
                     /locus_tag="ACG8ER_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS5049181.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="ACG8ER_000571"
     CDS             complement(23176..23655)
                     /locus_tag="ACG8ER_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5049182.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="ACG8ER_000572"
     CDS             complement(23728..24012)
                     /locus_tag="ACG8ER_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049183.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="ACG8ER_000573"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8ER_000573"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="ACG8ER_000574"
     CDS             complement(24356..25642)
                     /locus_tag="ACG8ER_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS5049184.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="ACG8ER_000575"
     CDS             25780..26451
                     /locus_tag="ACG8ER_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS5049185.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="ACG8ER_000576"
     CDS             26417..26905
                     /locus_tag="ACG8ER_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS5049186.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEAKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="ACG8ER_000577"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="ACG8ER_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS5049187.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="ACG8ER_000578"
     CDS             27866..28630
                     /locus_tag="ACG8ER_000578"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS5049188.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="ACG8ER_000579"
     CDS             28863..31076
                     /locus_tag="ACG8ER_000579"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS5049189.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /gene="dtd"
                     /locus_tag="ACG8ER_000580"
     CDS             31087..31533
                     /gene="dtd"
                     /locus_tag="ACG8ER_000580"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS5049190.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="ACG8ER_000581"
     CDS             31704..32963
                     /locus_tag="ACG8ER_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS5049191.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="ACG8ER_000582"
     CDS             complement(33052..33999)
                     /locus_tag="ACG8ER_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5049192.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /gene="hisS"
                     /locus_tag="ACG8ER_000583"
     CDS             34393..35685
                     /gene="hisS"
                     /locus_tag="ACG8ER_000583"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS5049193.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="ACG8ER_000584"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="ACG8ER_000584"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS5049194.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="ACG8ER_000585"
     CDS             37578..38453
                     /locus_tag="ACG8ER_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5049195.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="ACG8ER_000586"
     CDS             38719..40602
                     /locus_tag="ACG8ER_000586"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5049196.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="ACG8ER_000587"
     CDS             complement(40677..40961)
                     /locus_tag="ACG8ER_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5049197.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="ACG8ER_000588"
     CDS             complement(40951..41685)
                     /locus_tag="ACG8ER_000588"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS5049198.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="ACG8ER_000589"
     CDS             41814..42437
                     /locus_tag="ACG8ER_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS5049199.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="ACG8ER_000590"
     CDS             42629..43750
                     /locus_tag="ACG8ER_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5049200.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="ACG8ER_000591"
     CDS             43757..46885
                     /locus_tag="ACG8ER_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5049201.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLNLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="ACG8ER_000592"
     CDS             46904..50098
                     /locus_tag="ACG8ER_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS5049202.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFHQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="ACG8ER_000593"
     CDS             complement(50244..50951)
                     /locus_tag="ACG8ER_000593"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5049203.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16784 a         8954 c        10637 g        14886 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacaatc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagcaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttaat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccatcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg t
//
LOCUS       DAZUMO010000010        50995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000010 DAZUMO010000000
VERSION     DAZUMO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.010"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..549)
                     /locus_tag="ACG8ER_000594"
     CDS             complement(<1..549)
                     /locus_tag="ACG8ER_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5049204.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFL"
     gene            complement(625..771)
                     /locus_tag="ACG8ER_000595"
     CDS             complement(625..771)
                     /locus_tag="ACG8ER_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFS5049205.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(875..1186)
                     /locus_tag="ACG8ER_000596"
     CDS             complement(875..1186)
                     /locus_tag="ACG8ER_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5049206.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1188..1385)
                     /locus_tag="ACG8ER_000597"
     CDS             complement(1188..1385)
                     /locus_tag="ACG8ER_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5049207.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1779..3506)
                     /locus_tag="ACG8ER_000598"
     CDS             complement(1779..3506)
                     /locus_tag="ACG8ER_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS5049208.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3499..4692)
                     /locus_tag="ACG8ER_000599"
     CDS             complement(3499..4692)
                     /locus_tag="ACG8ER_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5049209.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4879..5523)
                     /locus_tag="ACG8ER_000600"
     CDS             complement(4879..5523)
                     /locus_tag="ACG8ER_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5049210.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQAKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5617..5751)
                     /locus_tag="ACG8ER_000601"
     CDS             complement(5617..5751)
                     /locus_tag="ACG8ER_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS5049211.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5753..7885)
                     /gene="feoB"
                     /locus_tag="ACG8ER_000602"
     CDS             complement(5753..7885)
                     /gene="feoB"
                     /locus_tag="ACG8ER_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS5049212.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7882..8355)
                     /locus_tag="ACG8ER_000603"
     CDS             complement(7882..8355)
                     /locus_tag="ACG8ER_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS5049213.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8756..8980
                     /gene="nrdH"
                     /locus_tag="ACG8ER_000604"
     CDS             8756..8980
                     /gene="nrdH"
                     /locus_tag="ACG8ER_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS5049214.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9139..11298
                     /gene="nrdE"
                     /locus_tag="ACG8ER_000605"
     CDS             9139..11298
                     /gene="nrdE"
                     /locus_tag="ACG8ER_000605"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS5049215.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            11348..12313
                     /gene="nrdF"
                     /locus_tag="ACG8ER_000606"
     CDS             11348..12313
                     /gene="nrdF"
                     /locus_tag="ACG8ER_000606"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS5049216.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12402..13541)
                     /locus_tag="ACG8ER_000607"
     CDS             complement(12402..13541)
                     /locus_tag="ACG8ER_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5049217.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13850..14563)
                     /locus_tag="ACG8ER_000608"
     CDS             complement(13850..14563)
                     /locus_tag="ACG8ER_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS5049218.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14817..15518)
                     /gene="nagB"
                     /locus_tag="ACG8ER_000609"
     CDS             complement(14817..15518)
                     /gene="nagB"
                     /locus_tag="ACG8ER_000609"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS5049219.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15752..17284
                     /locus_tag="ACG8ER_000610"
     CDS             15752..17284
                     /locus_tag="ACG8ER_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5049220.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17475..18677)
                     /locus_tag="ACG8ER_000611"
     CDS             complement(17475..18677)
                     /locus_tag="ACG8ER_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5049221.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18759..19448)
                     /locus_tag="ACG8ER_000612"
     CDS             complement(18759..19448)
                     /locus_tag="ACG8ER_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5049222.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19554..19922)
                     /gene="rplL"
                     /locus_tag="ACG8ER_000613"
     CDS             complement(19554..19922)
                     /gene="rplL"
                     /locus_tag="ACG8ER_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS5049223.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19975..20478)
                     /gene="rplJ"
                     /locus_tag="ACG8ER_000614"
     CDS             complement(19975..20478)
                     /gene="rplJ"
                     /locus_tag="ACG8ER_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS5049224.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(20682..20809)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20888..21577)
                     /gene="rplA"
                     /locus_tag="ACG8ER_000615"
     CDS             complement(20888..21577)
                     /gene="rplA"
                     /locus_tag="ACG8ER_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS5049225.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21681..22103)
                     /gene="rplK"
                     /locus_tag="ACG8ER_000616"
     CDS             complement(21681..22103)
                     /gene="rplK"
                     /locus_tag="ACG8ER_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS5049226.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            22446..23114
                     /gene="sdaAB"
                     /locus_tag="ACG8ER_000617"
     CDS             22446..23114
                     /gene="sdaAB"
                     /locus_tag="ACG8ER_000617"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS5049227.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23127..23999
                     /gene="sdaAA"
                     /locus_tag="ACG8ER_000618"
     CDS             23127..23999
                     /gene="sdaAA"
                     /locus_tag="ACG8ER_000618"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS5049228.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24090..25190)
                     /locus_tag="ACG8ER_000619"
                     /pseudo
     CDS             complement(24090..25190)
                     /locus_tag="ACG8ER_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(25282..26169)
                     /locus_tag="ACG8ER_000620"
     CDS             complement(25282..26169)
                     /locus_tag="ACG8ER_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5049229.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(26246..26791)
                     /gene="nusG"
                     /locus_tag="ACG8ER_000621"
     CDS             complement(26246..26791)
                     /gene="nusG"
                     /locus_tag="ACG8ER_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS5049230.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26892..27062)
                     /gene="secE"
                     /locus_tag="ACG8ER_000622"
     CDS             complement(26892..27062)
                     /gene="secE"
                     /locus_tag="ACG8ER_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS5049231.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27086..27238)
                     /gene="rpmG"
                     /locus_tag="ACG8ER_000623"
     CDS             complement(27086..27238)
                     /gene="rpmG"
                     /locus_tag="ACG8ER_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5049232.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            27400..28044
                     /gene="cbpA"
                     /locus_tag="ACG8ER_000624"
     CDS             27400..28044
                     /gene="cbpA"
                     /locus_tag="ACG8ER_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS5049233.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28100..28603)
                     /locus_tag="ACG8ER_000625"
     CDS             complement(28100..28603)
                     /locus_tag="ACG8ER_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5049234.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(28616..28662)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            29200..30165
                     /locus_tag="ACG8ER_000626"
     CDS             29200..30165
                     /locus_tag="ACG8ER_000626"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5049235.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(30264..31436)
                     /locus_tag="ACG8ER_000627"
     CDS             complement(30264..31436)
                     /locus_tag="ACG8ER_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049236.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(31423..32568)
                     /locus_tag="ACG8ER_000628"
     CDS             complement(31423..32568)
                     /locus_tag="ACG8ER_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049237.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(32582..33506)
                     /locus_tag="ACG8ER_000629"
                     /pseudo
     CDS             complement(32582..33506)
                     /locus_tag="ACG8ER_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            33623..34702
                     /locus_tag="ACG8ER_000630"
     CDS             33623..34702
                     /locus_tag="ACG8ER_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5049238.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            34699..35327
                     /locus_tag="ACG8ER_000631"
                     /pseudo
     CDS             34699..35327
                     /locus_tag="ACG8ER_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(35437..35664)
                     /locus_tag="ACG8ER_000632"
     CDS             complement(35437..35664)
                     /locus_tag="ACG8ER_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5049239.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(35767..38355)
                     /locus_tag="ACG8ER_000633"
     CDS             complement(35767..38355)
                     /locus_tag="ACG8ER_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS5049240.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(38375..39034)
                     /locus_tag="ACG8ER_000634"
     CDS             complement(38375..39034)
                     /locus_tag="ACG8ER_000634"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5049241.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(39083..39586)
                     /gene="trmL"
                     /locus_tag="ACG8ER_000635"
     CDS             complement(39083..39586)
                     /gene="trmL"
                     /locus_tag="ACG8ER_000635"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS5049242.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(39807..40673)
                     /locus_tag="ACG8ER_000636"
     CDS             complement(39807..40673)
                     /locus_tag="ACG8ER_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5049243.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            40782..41423
                     /locus_tag="ACG8ER_000637"
     CDS             40782..41423
                     /locus_tag="ACG8ER_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS5049244.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(41485..42846)
                     /gene="mgtE"
                     /locus_tag="ACG8ER_000638"
     CDS             complement(41485..42846)
                     /gene="mgtE"
                     /locus_tag="ACG8ER_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS5049245.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(42864..43763)
                     /locus_tag="ACG8ER_000639"
     CDS             complement(42864..43763)
                     /locus_tag="ACG8ER_000639"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5049246.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(43765..44562)
                     /locus_tag="ACG8ER_000640"
     CDS             complement(43765..44562)
                     /locus_tag="ACG8ER_000640"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS5049247.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(44540..45244)
                     /locus_tag="ACG8ER_000641"
     CDS             complement(44540..45244)
                     /locus_tag="ACG8ER_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS5049248.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            45375..45950
                     /locus_tag="ACG8ER_000642"
     CDS             45375..45950
                     /locus_tag="ACG8ER_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS5049249.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            46068..46745
                     /locus_tag="ACG8ER_000643"
     CDS             46068..46745
                     /locus_tag="ACG8ER_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS5049250.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(46824..48632)
                     /gene="pepF"
                     /locus_tag="ACG8ER_000644"
     CDS             complement(46824..48632)
                     /gene="pepF"
                     /locus_tag="ACG8ER_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS5049251.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(48808..49806)
                     /locus_tag="ACG8ER_000645"
     CDS             complement(48808..49806)
                     /locus_tag="ACG8ER_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS5049252.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(50032..50685)
                     /locus_tag="ACG8ER_000646"
     CDS             complement(50032..50685)
                     /locus_tag="ACG8ER_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS5049253.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15362 a        10648 c         8876 g        16109 t
ORIGIN      
        1 taaaaataga acaacaaaca tttgaatgat taaaaatatt aagacaccaa acacaaaatg
       61 atcaaatact tgatattgat gagcaacata agaaacaaat aaaccaattg taagtaaata
      121 aatcatgaat atagcagtaa gtttagaact ggactgttca acaatcctat ttatagctaa
      181 ttttctatat aaccaaataa acatcagcaa aaacaaagaa tcaagcatta tctggaatac
      241 tacataacca taccctttta tcccttcaaa tgaaaacata tacgtcataa ccatggaagt
      301 aataatagat ctgaaatcat caataagcat acataatagt aatttattta aaagcactat
      361 attagttttc ttcttttcct ttaatataaa ataacttccg attataggta tagctgcgga
      421 aacatcagta aataattcaa caaaaaaagt gagtggtatc aataaacata caaaaagata
      481 ttttcttttg ttaagatcag tattagtcat ataaaataag agaaaaaaga aaaagtttcc
      541 cacgaacatt cttaatagat aagcttttgt atcgatagta atcaagagat cccctcctga
      601 ttttaggata tcataaaaaa aattttagtt acacttttta aataatgaaa aaacacattc
      661 cactaaattt gatgccggtt ttccttgagg ttttgttcca gcacctcctt taattttttt
      721 aagctcttga tctgaacatt gtttagcatt tagtctattt ttttcttcca ttacgattcc
      781 ccctacaaac tataaatact tttatgtcac tgattcaata tacctatttt tctaatcaaa
      841 aaattttgag acatatttaa tattataagt atcattaaaa ttgagattta tctccataat
      901 ctgctcgata taaaccattt attccagcag ttcttccaat ttcactcaaa tttcttatta
      961 attcctcttg atattcagtg aatcttattt tattagtata agcagtaaaa tatagataat
     1021 tcaccaatct atttattaaa ggaccgggat ctaattcata tttttgtttt tctaatttta
     1081 aatatacctt ttgtaaaacc tcaagaatat cagaatattt aggatcttta cttatagata
     1141 tatcattata taattcatga acaatttgct tagcattttt tttcatttta gcacttccct
     1201 ggaattgctc cacctaaaaa accacctata gacattcctg caatacaagt agttgccttg
     1261 gcccaatcga ccgtacattt atttttagtg caatacactc catttccata atattttcca
     1321 ctatgagtgg tacccccata gataagttgt gtctctttaa tagacaaaat ttttaaatgt
     1381 ttcataatct aatcacccct aaattaatgt ttttagacta ttattctaac attcattaaa
     1441 tttcaaaaaa caattccttc ttgaaacgta gcaaaataat tcctgaataa gaatttaatc
     1501 acttttaagt atataaacta tgtatattct ttaatatttt ttgagaatgc acttcgtaca
     1561 taataaactt taatcacatt agtctttcat acctacgatc gatctctttg actcgcaccc
     1621 ataaattcat aatagtttgt cggcgtcttt ggacgtttcg aagatgagct acgcattctc
     1681 tttttttata gacaaaaaag atagagacga acatggagct tccaaatttg gatatttgat
     1741 ccaattttgg aagctccatt tatttcgtgt ctgtcttttt attttttcca gctttcgact
     1801 aattcttggt tctctttgat ccagtctttt gctgcttgct gtggatcttt tccattgttg
     1861 atttccaaca taactttttc catatctttt tctgaccaat ggaaattgtc taatactttg
     1921 taagcttctg gctggtcttc tttcaatcct tttcgaacca tcgtatggat ctgctcttcg
     1981 cttcccattc cgttttcagg atcttctaag tatttcagat catatttggc aaacatccaa
     2041 tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt tgatcgcttg acctagtgca
     2101 acggtcattg ctcctgaaga agatgtctct actttccagt ctttcaagtt gtcatatttt
     2161 tggatcgtgt tttcagcagc cgtaactaca ccagctcctg gctcgattcc tatgattttc
     2221 ttacctgcct gatcggttaa ctcatcgatc gaatttacat ccatgtaggc tggcactgct
     2281 aaaccaactt ttgctcctgt caaattggca cctaggtctt cgacttgatc tttatattgt
     2341 gcatattgtg aaccatgagt tttaggtagc caagctgaaa ccatggcatc tgactcgcca
     2401 tttgaaacag atttccacat gattgaatta tctaatggcg tcatcgttac atcatagccc
     2461 atttgtttca atacttcacc gactacattt gttgaagcaa cttctgtatc ccattcaaca
     2521 taagataagt tgatacgttt tgtttctttg gcagatgaaa aactccctat accaatcgca
     2581 cctaagatca ctactactac agcaccaatg atcagtcctt gacgtttttt acttttctgt
     2641 gttgttgctc ctgcagcttt tttattgttt ggctggttca aatgctgtgt aaaacggtca
     2701 atgatgattg ctaagataac taatgcaacc ccgttgacaa atccattccc gacttgtgct
     2761 ctttgcaatg ctgataatac gccgcgtcct aagccgggtg caccgatcat agaggcaata
     2821 acgaccattg aaagtgacag cattgttgtt tgattgatcc ctgccatgat cgtactttta
     2881 gctaaaggca attctagttt gaatagtttt tgacgacttg tgctcccgaa tgaatcagaa
     2941 gcttctacta attctttagg aacttgtcga atccctaaat tcgtaaaacg aacggttggt
     3001 ggcagagcaa agatgactga ggcaaatacc cctggaacca tcccgatacc gaaaaatgca
     3061 actgctggaa tcaaataaac gaatcctggc attgtctgca taaagtcaag gatcggtttg
     3121 ataatacttt gggctttact gctttttgcc atcaagatcc ctaatggtac cccaatgatg
     3181 atcgagatca cactagataa aagaacaagt gtgacagtat tcatcaaatc attccataga
     3241 ccttggttat aaataaacca tagaccgatt aatgtgaaca acgtaagtcc tggacgtttc
     3301 ttagaaataa agaaagctgc taccgtcaat aacaagataa acaaaggagc tggaatcact
     3361 aacagtaaat ttgtaatccc cgacatcact gcctgtccaa tcgtttgtaa aaatgaaaat
     3421 aggccagaaa aagttgtcgt aaaccattct gtgatatttt ctacccagtt tgccactggt
     3481 aattgatagt tcaatagatt attcatgttc actcacctcg cttgtttctg ctaatgcttc
     3541 aatcacacta cctcggatga cgacaccttt taattttcca tcctctacaa ctgcaagtgg
     3601 agtcggtgag tcataaataa tattgaaaat atcacttacc accatgtctt tcccaatcac
     3661 cgtcacattt tcatctagga agtcgatcaa tggacgtttt tgcttcctag cttctaacgc
     3721 ttgatctgca gtgatgatcc ctttcaggtg tctctttctg tcaacagcca tcagcatgct
     3781 gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt cgatattcgt
     3841 cgtcaatgca ggtaccatga tgttttgagc cgtaagcact ttggaacgat cgacttcttc
     3901 cacaaattca cgtacataat catttgctgg attggtcaat atttcttcac ctgttccgat
     3961 ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta aagcttcatt
     4021 caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt ccagtaattc
     4081 gtcttgcatt tctcgccgga tcagcggatc aagagccgag aacgcttcgt ccatcagcaa
     4141 gatctcagga tcatttgcta aagctctggc taggccaacc cgttgttgca tcccaccaga
     4201 taactgacta ggatattggt ctttaaatga aagcaaacta gaattttcta atgctttttc
     4261 tgctttttct tgtcgttctt ctttaggtac accacggact tctaacccgt attctgtatt
     4321 ttccaagatc gttcgatgtg gaaacagacc gaagttttgg aaaaccatat tgattttatg
     4381 acgacggact tctcgcaatt cttctttgct taacttcgat acatcttctc catcaatata
     4441 gatactgccc gaagttggct caatcagacg attcaataga cggatcaatg tggatttccc
     4501 actcccggaa agccccatga tcacgaagat ttctccttct tttacgtcaa aattcacgtc
     4561 atacacacca accgttgcac cggttttctt taaaatctcc gttttcggct ggtgatcatt
     4621 catcatatct aaagcggctt gtgtcttttt accaaatatc tttgttaaat gttttacttc
     4681 tacttttacc aaacaaattc tcctttttta tgtaaattca tttttctttc tgaaaaaagt
     4741 gaccacacta tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa tttgcttgtc
     4801 caaactgaaa aaaacttggc taaatctttg taaataaaag gtttagccaa gttaatattt
     4861 ttttaatatt tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa agtttgtcat
     4921 acgctgtagt gtatattcat ttccaataaa atgaacgatg tcccctgccg taaatttagc
     4981 ataggggcca ggtgaaacaa gcagttcttc attatgagaa attgcgataa ctgtggcgcc
     5041 agtcgcctgc cagatattca actcgctaat tgaacggtcc agatgcattg ctttttctga
     5101 taaagataat tcaaacggaa gtaaaggatt catcttatgt attcgcttcg cttgattgac
     5161 cagctgatcc aataattgag aaaaatgatt cagctcatct tgttgcttct ccacacttgc
     5221 catgatttct gcttttgtat cttggatgga ttgtacatct tgatattgca ataggaaatc
     5281 ttttgctttt tccttcgaag caacaaatgc cccacttcca tgccgtactt ccatgattcc
     5341 aagatctacc agcacattga tagccttgcg ggtagtctca ggagaagcat tgtacgtact
     5401 ggcaagtgtt gagcgggcat ggatcttctc acccacttta tatcggtttt caacgatacg
     5461 ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg acgatttttt tctttccaga
     5521 cattttcttt cctctttctt tttttacttt ccctcatctt actatgctct agcaaaataa
     5581 gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat cgcttgatct gttccttttt
     5641 tactgggcaa gctgtttgac agtcatcaca attcttaccg gattttaaac gagtatagac
     5701 aattgcgcct gcacttccaa agattaacac acttaataag aatgtcgcca tcctattcct
     5761 ctccttctaa tgacaatata gtataaaact ggttcttgga ttttggctga cggaacaaaa
     5821 cataaacgag tcctatcatc agtaaaatag caagatacgt ccctaacgct acctgtccac
     5881 cctccaataa gtgtcccaac tgataaatca caaaactaac taaataagct aaaccgcatt
     5941 gatagcctat ggcgatccaa gtccatttct tggcattcat ttcccgatgg atagccccaa
     6001 ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa caatgaataa gctgctgcgg
     6061 gagtatatgt gtgttgtagc acttgccaga tttctctgcc attttcggat acttccgcat
     6121 gaccaaacaa aataccaaat gtgccaataa tattttcttt agcgaccaat cctgtaatcg
     6181 ctgcgactgt tccttgccag tttccccaac caagcggctg aaaaacaact gccaggattc
     6241 gcccgaaaaa ggctaagata ctttggtctt cagaaacagg ttgagcataa aaattataag
     6301 aagagctgaa ccagatcacg atactagaaa caaagataat cgttcctgct tttttaacaa
     6361 aagacttgct gcggtcatag gtttgtctta ggaccgctga ccattgagga aaatgatatg
     6421 cgggcaattc catgataaaa ggagctgtat ctccagcaaa caaacgtgtt tttttcaagc
     6481 tgattccaga gaaaataatt gcagtcatac caagaaaata ggcagaagga gctacccaag
     6541 attgatgagg aaaaaaggcc cctgctacta aggcaataat cggcaacttt gcagaacaag
     6601 gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg ctcgttttca atcgtgcgac
     6661 tggccatgac gcctggcact ccacatcctg tagcaatcag cataggaatg aaggattttc
     6721 ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa agcgatcctc gccatatatc
     6781 cgcagtcttc cagaaagctc aagcataaaa acaatataat caattgcggt agaaatccta
     6841 acactgctcc cacgcctgcg atgatgccgt tcaagatcag ctcctgcatc catcccgcta
     6901 cttgccattc agctaacaat ctgccaacat gttctggtaa ccatgtacca aacaatgtgt
     6961 cattgatcca atctgtcccc attgttccta ctgtttgaat agccagataa tagatcagcc
     7021 acatcacaaa tgcgaagatc ggcaaggcta accaacgatt cgttgcaatc tgatcgattt
     7081 tgtcactcat cgataattgg aaagaatcat ttgacacaac acagagtgta catagttggg
     7141 taataaattc ataacgttca ttgaccagaa tcgtctcact atcgtcgtcc aatagtttct
     7201 ctgttaactg gatcagttgc tcgatctctt tttgctgaga aactgctatc tcgtaatcct
     7261 ctcttacctt ctgatctcgt tcaaaatatt tcattgcata ccagcgtttc ttgccgcttt
     7321 gttctcctag tacatcttca atttcatgta aagctgcttc taaacgtgga tcatatgtac
     7381 agggaacagg aatagaaggc cgcttttttg cagactcctg aatcttttcg attagttttt
     7441 cgattccttg ctttttcaat acgctgattg gtaaaactgg tacaccaaga ccataggaaa
     7501 gcttttctag attgattttt tttccttctt ttttgatgac atccatcata ttcagaccaa
     7561 tgatcatagg aatgtctaaa tccaataact gcgtagttaa ataaagattt cgttctaaat
     7621 tggtagcatc aataatgttg atgatcagtt caggctgttc ctgaaccaaa taatctcttg
     7681 caatcacttc ttctggtgta taaggagaca aagaatagat gccgggtagg tcctgaatat
     7741 gaatgttcgg attttttttc aacgatcctt cttttttttc tactgttacg cccggccaat
     7801 tccctacata ttgattcgaa ccagtcaaga gattgaacaa gctagttttc ccgctgtttg
     7861 gatttccgac taacgcgata ttcattcttt ttcctccctt tgtatcaaga ttagttcggc
     7921 ctctgacttt cgtaacgtca gttcataccc tcgcactgtc agttcgattg gatctcccag
     7981 aggggctagt tttcggataa ctatttttac atttcgcgtc aatcccatat ccattaaccg
     8041 tcgcttcact gcgccttgtc cgtggattgc tacgacgatc ccagtctctc ctgcttttaa
     8101 ttgatcaaga gaagtccagc tttcagagga agcagtcttt ttattgacaa agatttgacc
     8161 tagtatttcc cgacttactg ccactcgatg actttttacc agtaaaatgg cattgttgcc
     8221 tgataaattg acgacgacga ttttacttcc ggcaacgatc ccgatatttt gcaaatggcg
     8281 ttttgttttt tcttctgttt cgattcgttc gacttgataa acgaaattct gctttgcctg
     8341 cgctaaggtc tgcatattga ttcctctctt gtgaaataaa tcatgtatta aaatcattca
     8401 ttatatgttc taattttctt acgtgttacg caaataaatg agaatcattc tcacatagaa
     8461 tactctcggt aattgagaat gtcaattata cttgtgatat tatgagcgaa tctttagtat
     8521 tgatagataa aaatgagaaa aaaatatttc ttcatagttt tccacaattt tttctctatt
     8581 aaatttgcta ttttcccaca cgatacttgt tttcagctct tttttcaaaa acacaaaact
     8641 tagtattcca cagtttccac aacacaatat gtagtgtttg ttgtttgact tttttactaa
     8701 gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac tcaccatgaa
     8761 cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac ggtttttaac
     8821 agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag cagtagactg
     8881 gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag aaacagttat
     8941 cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa agaatcaaaa
     9001 cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa acttccaaat
     9061 ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc aaatacgaaa
     9121 tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta tttcaagctg
     9181 aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga taaagaagct
     9241 ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttct atctattatg
     9301 gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat tcaattgtat
     9361 tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt ttctttcaaa
     9421 tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa cgacggttcc
     9481 gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt tgcgaacggg
     9541 gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata tcagccagct
     9601 acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc ttgtttcttg
     9661 actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc tttgcaattg
     9721 tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc cggcgctcct
     9781 atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt attcgaagat
     9841 agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt ttatttgaac
     9901 gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc agatgaaaag
     9961 attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta tgaattgaca
    10021 cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga atatggtgtc
    10081 ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa tccaaacatt
    10141 cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt acaacaagaa
    10201 tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc aatcgatggc
    10261 aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc ttctgtgatt
    10321 aacggcaaac aggaatatga agtattagga acggatatct catgtaactt aggctcgaca
    10381 aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac gatgactcgt
    10441 gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat ccaaaacgga
    10501 aataagctga atcatacgat tggattaggt gccatgggct tacacactta tcttgcaaaa
    10561 gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta cttcatgctg
    10621 ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa acaagttttc
    10681 cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata cgtaactgga
    10741 gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt tatcccaact
    10801 gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta tcatcaaaat
    10861 cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag tgcaagtatt
    10921 catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa aatttattac
    10981 ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta tgatatggac
    11041 atgcgtaaag taatcgatgt ttatgcaatg gcacaacaac acgtcgatca aggcatgagt
    11101 ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa gacaacaacg
    11161 aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa aggaatcaaa
    11221 tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag caatcaatgt
    11281 gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta attaaaggag
    11341 cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg aagatatcat
    11401 tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac gtatcccact
    11461 ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata cagtaggaca
    11521 tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca tggatcagtt
    11581 gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc aattcatgga
    11641 atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga aaaaagaaat
    11701 cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag ctgagcgtat
    11761 caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg tctttttaga
    11821 aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa acaacaaatt
    11881 ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac atggaaccta
    11941 catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac aagaaacgtt
    12001 aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac gttatacaga
    12061 agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac gctataatgc
    12121 caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg ccaatgatgt
    12181 caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact tcttctcaca
    12241 agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg attatttgat
    12301 tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa aagagtttag
    12361 cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact tgtttcttcc
    12421 agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa taaatagatt
    12481 ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca aatcagcatg
    12541 cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt caaaggatgg
    12601 atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa ttgttggatc
    12661 actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa accgacaatg
    12721 attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt gattccgcaa
    12781 atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa aaagctcatt
    12841 acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa tgtttcgttt
    12901 aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa gtgagacaac
    12961 gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg aacggtaaat
    13021 aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc agataaactg
    13081 actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa ttgttcttcc
    13141 aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt gcttgtttca
    13201 tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa ctgaggcact
    13261 acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa taaaaaaccg
    13321 aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa catataatag
    13381 ataaaaccag caacaagcaa tggtaaaaaa actgccgaaa gcattttcaa tattggacta
    13441 aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac tagctccact
    13501 gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct ccaaggtcct
    13561 ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc atttgcttaa
    13621 aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac aaaaacacgg
    13681 tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg gtgttcaaaa
    13741 gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat ttttatttgg
    13801 ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt tattgcgcaa
    13861 tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg atcacatcgg
    13921 cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact cggtagtcat
    13981 caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct tcaaaataat
    14041 cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac tttttagtcg
    14101 taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg ataaaactga
    14161 tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca attgcccata
    14221 aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc gttcccgcgc
    14281 ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct gaacgaatcg
    14341 ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg atctcaactt
    14401 ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc atacctgctg
    14461 gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata gcgatcccta
    14521 aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca ccctccagtc
    14581 caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct cttacgaaaa
    14641 aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg ttcaatcagc
    14701 tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc ctttgtcttc
    14761 tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat tgaaaattac
    14821 aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt ttgcaataca
    14881 cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc atcagctttg
    14941 ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc gattcccatt
    15001 gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt tgcattgatt
    15061 gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc aggttcattg
    15121 aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg atgtgcatcg
    15181 atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc atctggtaca
    15241 aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg acggtagctt
    15301 tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt catttcactg
    15361 aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct tcctgttgcc
    15421 aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa tgcttttttt
    15481 ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc tttatatttt
    15541 ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat agaccaaatt
    15601 gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca taagatggtt
    15661 caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg acgctttatg
    15721 tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa acaacattac
    15781 ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa atgctggcga
    15841 acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta aaatcatcac
    15901 tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc acaaaggtta
    15961 cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc taaaaactgc
    16021 gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa catatagcga
    16081 gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat tagaccaagg
    16141 agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg ggatcgatgt
    16201 cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa aggtgatttt
    16261 agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa ccaatcactt
    16321 agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg gtgcgtatat
    16381 cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg cagacatcga
    16441 gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac aaaaggaaca
    16501 aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg aaaagaccga
    16561 agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa gccgacaaaa
    16621 acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag ctgtctttga
    16681 ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt tagtcatcgg
    16741 atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg aaaatattgc
    16801 cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga tcggtgagat
    16861 tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt acttttcaca
    16921 agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca gtcaatttcc
    16981 aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag ttaatcaatt
    17041 agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga aacttgcaca
    17101 gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc atatcgatca
    17161 agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag taatcatcgt
    17221 ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga ttgaaccaaa
    17281 ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt gtcacaactc
    17341 cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca aaaaacggca
    17401 aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa cagcttttca
    17461 caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa aacaactgtt
    17521 tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc taaccagcca
    17581 tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat aaagtacata
    17641 aacataaata cagtattatt tcggttgctt gctgcttcac ctagcccttg tactcgtgtt
    17701 tgattggcga cttgaccaaa ttgattcccg acgtctaata aaataatagc taaaagcaaa
    17761 atggccaaat ggcttccgcc aaccaataaa gcaataaagc ttattgtttg cataacaagt
    17821 cccatataca cgatttttct ttcagaataa gaatctgcca gtcgtcccac gataggtgca
    17881 gcaactgctc cagacaaacc aaaaatcgct aatatccctg cttcgaatgt tccccaattg
    17941 taggcgggac tgctgatata aaaaatcaac gtagaccaaa agatcgaaaa tgttccaaac
    18001 atgaaaaatc cactgactgc cgcttctctt aatagacgtt gagaagcaac taaatgcggc
    18061 aaacttttaa gtgaatcaag gtatgaaaca gctgtactac tttgctggac agaacgcggc
    18121 attttccagt aaagcagaag tgtaagaatt ccgactgcac ataaagcaaa taaatagatc
    18181 atccgccaag aaaaaagact ggcaacaata ccagataccg tccgagaaag caaaatcccc
    18241 acgagtaaac cgctaagcaa tgtacccatg accttccctc ttgcttttgg tcccgctagt
    18301 accgcaccgt aaggaatgat gatttgcggc acgatcgaaa gtaatcctat taaaaaagaa
    18361 gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg ccaacagaga gacggcagat
    18421 aacgctgcca ttcgaatgat caattttgga cgatccacca cgtcccctaa aggaactaaa
    18481 aacagtaacc ctaacgcata gcctaattgg gtcaacatcg tcaatatccc gactgcactt
    18541 gtgctaacag ataaactagc tgctactttt gttccaatcg gctggatgta atacatattg
    18601 gcaacaacaa cgccacaagt gattgataat agtaaaataa gcgaagttgt taaaggtttt
    18661 ttttcattac tcttcattga tttcccccat ttcctatctt agtttacaac ttatggaaaa
    18721 gattggaaat agaaaaatac aactattcgc cacacttatt atttgtgctt ttgttttcct
    18781 tgttttttct tgcgatcttt ataaatgaag acaactaacc ctactagcac agtaacactt
    18841 gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca gagcttctcc accacacgca
    18901 tacaacacag acgaagggat gactccaatc acgatcgaca atatgatttg ctgtagaggc
    18961 aatttcatcg catctgcagc aaaattgacc atagaagatg gaataaccgg tatcatatag
    19021 cctaacatta cacctatttt tggatgtttc atttgtcgga ttgcttgcac ccaatgattc
    19081 gtttcggttt tcttatctaa aaattttaag cgatgcataa ggaaaattgc agtgatgtta
    19141 ccaagagcat tacccaaaat gttgatgatc gcacctgtca gtggtccaaa gctcaaacca
    19201 gcaatgactc caactactga agtaggaata cctggaacag cacacatcac cgctgtcaat
    19261 acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa tatgtgcgag tagctctgcc
    19321 ttactcgctc tcggatttaa aaataattgg atgtctgtct gatattctaa atacaaccga
    19381 tagcccacta ccagtatcag aatgattccc aacgagatca atatcaatcg gattattttc
    19441 tttttcatat ttcctcctga aaaaaatcta tattcattaa ataatagttt aacataaaaa
    19501 caaaaaagca ccgaaatcca acataggatc tcagtgcttt taaatagtaa aaattattta
    19561 acagttacag atgcgccaac ttcttcaagt ttagctttca attcttcagc ttcttcttta
    19621 gatacgcctt ctttaactgg tgcaggagcg ccatcaacta cagctttagc ttctttcaag
    19681 cctaaaccag ttgcttcacg aactgctttg ataactttaa ctttttggtc tccagctgca
    19741 gttaattcta cagtgaattc tgtttgttct tcagcagcag cagcgccacc agcagcagct
    19801 acagctacag gagcagcagc agatacgcca aattcttctt caatagcttt aactaggtcg
    19861 ttaagttcta ggatagttgc ttcttttaac tcagcaacga tgttttcaat gttcaatgcc
    19921 attataattt cctccgttta attgttcttt tttatttaat gctttatgcg gcaattaagc
    19981 tgcatctcca tcttctttgt ctgccactgc tttgacagcg taagccacgt tgcggactgg
    20041 cgcttgtagt acagatagca acatagatag aagtccttcg cgatttggca attttgccaa
    20101 tgctgtgatt tcttccaatg aagagacttt accttcgatg ataccgcctt tgatttctaa
    20161 tgcttttgct tctttagcaa attcgtccat gattttcgct ggtgcgacta catcttcgtt
    20221 actgaatgca acagctgtag gtccagtgaa tacttcgtcc atgccttcaa ggccagcttt
    20281 tttagctgca cgtgaaagaa tagaattttt gataactttc atttctacgt tagcatcacg
    20341 aagttgttta cgtaaacgtg ttacttcatc aactgttaat ccgcggtagt caacgactac
    20401 tactgatgca gctgctgtaa atttttcgct tacttcatca acgattgctg ctttttttgc
    20461 gataattgct tcactcattt ttgagtttca cctcctgaat tttgatggaa gagttttttg
    20521 cactgaaaag gcaagtcact tttatagtta ctttgctcaa ccagtattat tcatcaccta
    20581 ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa ggaaactcct ttcgctttgc
    20641 tcaaccagta ttgttcatca tctcttcact tcgttcatag tgattcacaa taaaaaactc
    20701 tatgccaccg cagacataga gggactattg aatatattca ataaacgtcc tcggtaggaa
    20761 attaaggctt gatgccacct actgtcttcg gtacaagtta attattaacc tcaactagct
    20821 tagcatgcta aactagttga gtcaagaatt atttttcagt tggatcaatc aacattctaa
    20881 tatttaatta gaaagaagct tggtcaacat ggataccagg tccaaatgtt gttgtgatcg
    20941 ttacattttt catgtattga cctttagctg ctgatggttt agcttttagc aatacatcat
    21001 ggattgctgc aaagttttca actaattttt catcgtcaaa tgaaactttt ccgattggta
    21061 catggatatt tccagctttg tccacacggt aagttacttt accagctttc acttcgttta
    21121 ctgcttttgt tacgtccatt gtaactgtac cagttttagg gtttggcatt aagccttttg
    21181 gtcctaatac tcgtcctaga cgacctacag tagccatcat atcaggtgtt gcaacaacta
    21241 catcaaagtc aaaccatccg ttttggattt tttgaaccat gtcgtcgtca cctacgaaat
    21301 ctgcaccagc tgcttcagct tcttttgctt tgtctccttt agcaaaaact aaaacagttt
    21361 gtgttttacc agttccgttt ggtaatacaa cagcaccacg gatttgttgg tctgcttttt
    21421 tagggtctac gttcaaacgg tatgcaactt caactgttgc atcaaatttt gcaatgtttg
    21481 tttcttttgc taaagctact gcttcggcaa caggataaac tttacttgaa tcaacttttt
    21541 ttaatgcatc ttgcattttt ttgctctttt tagccatttt ggttcctcct tgattgtggt
    21601 tataacggtt gtacctccca cgtgttctat atctttcaat atagagccaa ctgagaagct
    21661 gctgtcttag ggtgtataga ttattctaca gtgatcccca tgcttcgtgc agttccttca
    21721 accatgcgca ttgctgcttc aacgttagct gcgtttaggt cttccatttt taattcagcg
    21781 atttctctta cttgatcgct agaaactttc gcaactttat ttttgtttgg ttcgcctgaa
    21841 cctttttcga ttttagctgc ttttttcaat agaacagcag ctggcggtgt ttttgtaacg
    21901 aatgtgaatg aacggtcttc gtatacagaa ataacaaccg gaatgatcaa acctgcttga
    21961 tcagctgtac gagcgttgaa ttcttttgtg aatcccatga tattgatacc cgcttgacct
    22021 agtgcaggac ctactggtgg agctggagtt gctttacctg caggaatttg caatttaacg
    22081 attttttcta ctttctttgc cacgagacat acctccttaa gtccgtgatg tggttaattg
    22141 gagatgcata tttctcctcc cacgttttat ctgatctgaa aaaatccaga caaataatgt
    22201 gcaaaacaat gcacactggt acagtatagc aaatttattt gttttttcaa gatttttttc
    22261 tgctattttt acattttgct tgaataaacg ctaaatttat tctaaaaaaa caacaatttt
    22321 tcgttctcat ttcttctttt atgttacact tgcgctagca tggttcaaca gatgaaagca
    22381 aacaataaca cggtttttta gacccttgta caaagattcc tacataacaa aatgaggtgg
    22441 agaaaatgaa agatttaagg tatagaagtg tctttgatat tattggacct gtgatgattg
    22501 gtccaagtag ctctcacact gccggagctg ctagaatcgg gaaaatcata cgtagtatat
    22561 ttggcgaaca gcctgattct gtagatattt atctatatga atcatttgca aaaacctatc
    22621 gggggcacgg aacagatatt gcattagttg gcggcctgtt agggatggaa cccgatgatg
    22681 aacgtttagc tgattctttg aaattggcat atgaagccgg tatggaagtc ttgtttgttc
    22741 ctaagagtga aaaagccgaa catccaaact ctgtaaagat gcttctaaaa aaaggaacac
    22801 gaaagctttc agtcactggc atttcgatcg gcggtggcaa tatccagatt tctgaattga
    22861 atggattcaa aatttcttta agcatgggta ctcctacctt gatcgttgtc caccaagatg
    22921 ttccaggtat gattgctaaa gtgactagta tactttctga gaccaatacg aatatcggca
    22981 ctatgacagt gacacgggaa tcaaaaggtg aaaatgccat tatgataatt gaagtagatg
    23041 atcctcaagt agaagaaacg gtaaaaagac tcaaacagct gccaaatatc gacagcgtaa
    23101 attatttcga ttaaggagct aaaaaaatgt ttttatcgat tgaagaatta gtagatcaag
    23161 caaaagctta tccaagtgta gcagaattga tgattgcagt tgaaatggaa atgagtggcc
    23221 gttcccgtag tcacatcatc gagatcatgg aaaaaaacct ctcagtaatg gaacaatcaa
    23281 tcaacgaggg aaaagaaggt gttacctccg tcacaggaat taccggtggt gatgcaaaaa
    23341 aattagatga ttatttaaat gaggcagact ttttaagtgg agaaacaatc ttaaaagctg
    23401 tacgcaatgc tgtagctgtt aatgaagtca atgcaaaaat ggggttgatt tgtgctacac
    23461 cgactgccgg aagcgcgggt gttgttcctg gtgtgctgat ggctgtccga gatcggcttg
    23521 atttgacaca tgaacagcaa ttggaattct tatttaccgc tggcgctttc gggttagtca
    23581 tcgcaaacaa cgcttctatc agtggtgcag aagggggatg tcaagcagaa gttggttctg
    23641 caagtgcaat ggcagcagca gctttggttt gtgcaaaaaa tgggactcct catcaagcag
    23701 cacaagcagt agccattaca ttgaaaaata tgatgggact gatttgcgat cccgttgcag
    23761 gcttagttga agtcccgtgt gtcaaacgta atgctttagg ctcttctcaa gcttttatct
    23821 ctgctgatat ggcattagcg ggtatcgaaa gtgtcattcc gccggatgaa gtcgttgctg
    23881 caatgtacca agtcggacgt caaatgccgt cgattttcaa agaaacagca gaaggtggct
    23941 tagctgttac gccaactgct aaacgacttg cggaagagat tttaaataag agggcgtgat
    24001 aaagctcctt aatttcgagt attaagagat tagttatctt cataacaaaa aacgaagaga
    24061 ggactaattg cccttctctt cgttttttat taatccatca atttcgtatt ttttacttca
    24121 tcgatcgttt ttgttcctgc aagttgcatc gtgatcgaca attctttgtt caggtgttca
    24181 aaaacagatg ttactccttc tgccccacct agattcaacc cataaatcac tggtcggcca
    24241 atcgcaacta agtcagcacc acttgccaga gctttgaaga catgctctcc tcgacggaca
    24301 ccagagtcga aaatcactgg tacacgtttg ttgactactt cggcaatttt tggcaatact
    24361 tcaaaggacg caggacctcc atctagttga cgtccaccat gattggatac ccagatacca
    24421 tctgcccctg cagagatagc gattactgca tcttctggcg attgtattcc ttttacgata
    24481 actggaagat gggtgatttc tttgattgtt ttgatgtcat ctggtgtcaa gccttgtttt
    24541 gcagctgcat aaatttctgc gattcctttt ccttcgccat taccacttgc actttgttcg
    24601 ctatacgcag ctaagttagg cattggtaat ggaaattgga attgattgat gacgtcttct
    24661 tcacggtaac caccgagcgt tgaatctgcc gttaaaatga tagctttagc ccctgcttct
    24721 actgctttgt tcaaaatgaa ctcattgaat ctgtcgtctt tactcatata taattggaaa
    24781 aactgcggtg cgcctggcgc tgcatcggct gcgtctttga ttgtcgtatt tgcataagtg
    24841 ctgatggaaa agattgatcc agcagcagct actcctttag ctgtatcagc ttcccctttt
    24901 tcatgtgcca atccttgggc cgctgatggc gcttggataa tcggtgtatc taattcaatt
    24961 ccgaaaacag atgtatgcaa atcggctgag tcgattccgc gtaatatccg tggcatgatt
    25021 ttctttgtat taaaagaagt cgtattttct ttcatcgtcc actcatcttc agaaccaccg
    25081 cgaatatagc caaaagctcc tgcttccatg cgctctttta cacgaccttc tagttaggca
    25141 atgtttacaa tatcgattgg atgttcttca ttacttgtat gataagtcat agtagatcct
    25201 tctttcatct tcttgattac atataaatca tactaaactt acaaaaagta agcaaacatt
    25261 tactctcaga tacttttatc gctattcttt ccaaataaat cgacttacaa gctgatctac
    25321 ttcttggttt tcatgaagca tactatgaga ggcattttca cctgtcacta ctttttcctc
    25381 tatttcattt ccgcgttgct tcaacaacgc atagaccgct aatgcactgt ttaacggtac
    25441 tgttccatca ctcaagtctg tttcatctag ttgaccagca atcaagaaaa atcgagtggt
    25501 tataggaata ttaccgatca tttgctggta atcttgatag cggttagaaa cgacagcagg
    25561 cccctttgct aactcatctg tcagagactg cgcactatca tcgacaaaat cattaaaagg
    25621 cgcgccaatt gctgttaatg tctgaacttg tggcaatgac gtatcctgtc catacgttcc
    25681 taaatagcgt agtgcactaa ctcctcccat ggaatgcccc actatgttga ctttatctat
    25741 gccgtagttt tctttcagat acaaaagaca agttttgatc cattctgctt gattccactc
    25801 gttattttta ttatcttcaa acaatacttg gaccatcgga ttgtctgctt ttccagacaa
    25861 atctcctgtt gcttgtattt gtccatctgt tcctactgtt aaaaccaatt cttttactgc
    25921 ttgctgattt ttttccaaac gccgcaacat ccctttaaag gaattttctg tcccgctgta
    25981 gccgtggaaa aaaagtgttg gaatagtggt tttttctatc actttactgg attctgccgt
    26041 tttttcttta tcagaaactt cagtagtatt tatcttcgtc gtctggcaac cacttaacgc
    26101 aaacaaactg ataaggacta aagctgctgc agttgcacgt ttgcataaat ttttgattgg
    26161 ttctgtcata cacctcttcc tttccatata gaaaaaagaa agacttgaaa ggccaatcat
    26221 atgatcgaca tttcaagtct ctttattaat cgattttatc tacttgatcg aaatcaagtt
    26281 ctgtactggt ttcacgtcca aacatatcga tgttgacttt caatttttct ttttctccgt
    26341 caacttctgt tacttgtcct tctaatccag caaatgcgcc ttcgatgatt ttcacgactt
    26401 cgccaacttc tacttctaga tcattttgtc gcaggctcat accgatagaa cgcaagatat
    26461 gattgacttc ttctggaagt aatggtgctg gtttgcttcc ggctccgtgt gaaccaacga
    26521 atcctgtcac accaggtgtg ttgcggacaa cataccatga agcgtctgtc atgatcattt
    26581 caacaagtac gtatcctggg aatgttttat gaacgatttc tttttctttc ccatttttga
    26641 cttcagtttc tttttcttca ggaacaacga cacggaaaat aaagtcggcc attttcatac
    26701 tttgcgcacg agattcgata tttgctttta ctttgttttc atatcctgaa tatgtatgca
    26761 agacatacca gtttttttca aaagattcca ttgaaggctc ctcatttcat tgtaaaataa
    26821 aaaaacctca gtacacccga agtttttctt tcgcttcatt atagcactaa aagtacgttg
    26881 tggctagcga gttattttaa aatccagcca aataagcttt ggatggctgt atccattacg
    26941 taaaacatca atccgaaaat aatagaagtt tcgatgacta ctaacgtatc tttacggagt
    27001 tgttttctgc ttggccaagt tacttgtttg atttcatctt ttacgctttt tagaaatttc
    27061 atgagacaac ctcccttgct actgtttatt tcgtttcttt atgtaatgta tattgattac
    27121 aatatttaca aaacttattg atttccaaac gttcgccgct ttttccttca ctgactgatt
    27181 ttgtgtagtt gcgagaccca caaacggaac aagcgagtgc tgcttttttc tttgccattt
    27241 acttctgccc ccttgctgag aatcagactt atacttcata acagtatcac gtaacttttt
    27301 acttgtcaat ttgtgaaata aactatctat tgttttggat aaaattatga tatgattaat
    27361 ttgaaattaa gctaaaatga gacaattgga ggtatagcga tgctattaaa aacacttgta
    27421 tataagaaaa aagatctgac aaccgttaca gagactagta cattagaaga agcattaaaa
    27481 attttagaaa cttccggata ccgttgtgtg ccgatcttag attcttcagg taatattttc
    27541 cgtggaaaca tttacaaaat gcatatctat cgtcataaag caaatggcgg tgatatgacg
    27601 cttcctgtca ctcatctttt gaaaaacgct acaaagttca tttacctgga tacttctttc
    27661 ttcaaagtat tctttacgat caaagagctt ccttatattg ctgtccttga cagtgaaaat
    27721 catttttatg gtattttgac acatagtacg ttgcttaata tgctctctca atcttggagc
    27781 gtagaacaag gaagctatgt actgacaatt gcttccacag gaaagcaagg agatttggct
    27841 gctattgcaa aaatcatttc aaaacacagc agcattgcca gctgtattac tcttgatatc
    27901 gaaaaagacg aatacattcg acgtacattg atcacgctac cggctaacac tgaagcgaaa
    27961 gtatgtgaaa cgatcgtcaa ccacttagag aaaaagaatt ataaagtggt agaaattgaa
    28021 aatttgcaaa accaagaagc ataatcaaaa agcgaaaaaa gaaaacagct aatctgcttt
    28081 cttttttcgc tttttataat tagacacgct tttcaaaatc aagcgcgcga tctaaataat
    28141 acatcaatgg tgctgaaaca gccatgacga taaactcact taatgcaagc gtgccatagg
    28201 ttgtccaaaa tgcttggcca cctgttggaa ctaacatata agcaatcagt cccatgctga
    28261 tggtaaagaa gatcgtgttc aatactaaac gcatctttac atttttcact ttgttttgca
    28321 ataaagctgt caggcctaaa gaaagcaatg tttggccgcc accaaaaacc acatccatga
    28381 taccaaaacc aaagaaggca ttatagacga tgaccccgcc taaaactccc caaagcaatt
    28441 tacggttaaa gacaactaaa tgattcaaac tttctgaaat acgaaactgg atcggccctg
    28501 aggatacagg cgcaactaaa atagttaacg ctagataaag agccatgatg atgccattta
    28561 caacgattac ggataaccgt tgtctgcttt tcgttttttg cattttctct cctcttttct
    28621 cctagttttt ttaacgcggg atggttgatg aaccgcggtt atgtgaaaca aacaataatt
    28681 ataacagact tttcatttat ttcaatccgt cttatctgct tttaaaaaag caaaagctta
    28741 aacaatgtaa acgtttgcta aaaatcgtgg gatatggtat aaagtaagtg taaggggtca
    28801 taagaattcc cagtaaagca gacgaagtag gatctgcaag aacagaattg ttgtgaaact
    28861 taaataaata aaaaaggatg tgggaatttg agcaacctct tactagagaa cggtctagca
    28921 agcagctgaa gatctaagtg gacatgccga accttggaac ttctccttac gtaagtaatg
    28981 gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt cggacctttt tcattttcct
    29041 agacttctgc tataataacc aagaattaat tttgtaacag atttttttga aaaaacaagt
    29101 ttctttcatt caaaaaatag aaaaagcttg ctaattattg gttaaccagc aaacaggagt
    29161 attaaaatat cttgttttat tgaatagcag gaggaaatca tgaaattcca ttatcatata
    29221 ttgatcaatc catcggcagg cagcggaaat ggctataaag tagcagaaag aattttaccg
    29281 gtattaaaaa ataagcatat tgactataca cttcattata gtgaatacaa aggacacgat
    29341 gctgagattg ctgaaacttt ggcaaaagaa acattgattc cttggataga agaagaacag
    29401 aaaacaatcg atactttccc ccttttgatc atcgtaggag gtgacggcac cttacatcag
    29461 gtacttgata ctttctatca gatggaagta gaattcccag ttgcttatat tccagcaggt
    29521 tctggagatg attttgctcg aggggcaggc cttccaaaaa atcccaaaaa aggacttcag
    29581 ctgattttag cagctcaatc accagaaaaa gtccacataa tggcttatga agaaaaaata
    29641 agtgaaaaaa aaggaatagc tgttaataat ttcggtatag gattagatgc agctatcgtc
    29701 catgcgacaa atcattcttc cacaaaaaag cgattgaaca aatacaactt aggatcattt
    29761 tcgtatttat tctctattct ccgtgcttta ttcactcaaa aaggatttcc aattctggta
    29821 gatgcaggag gaaaacaatt aagtttttca aacgcatttt tatgtacagc aacgaagcat
    29881 cctttttttg gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa accagtcatt
    29941 gattttgtcg tggtcgaacg aatcaatttc tttaaaatct tgtggctcat tcttttactt
    30001 atacagaaaa aacaattgaa atcgaaatat ttccatcact acacaacaaa cagattacgt
    30061 atcgtttcaa caacaccgca acatggtcaa gaagatggag aagaaatgga aaaacgacct
    30121 tttgatatca caataaccac aactaaacag ttgttttggt gttaataaaa gaggttttgt
    30181 cagaagctac atctggaaca ccgtttattc ggaaaaaagg actgtgacac gacttttgtc
    30241 acagtccttt ttttactatg cagttaattc tttgcccaat tgggttgcca tcgatagttt
    30301 ctttctttac ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc aactaagaca
    30361 caaccattga accaaaaaga ttctgcaaat gtttgattgt aactgagtgt ttcactgatc
    30421 aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt
    30481 tctggcaagt attctgctgg tacaaacacc ccaccgataa agcaactgcc tgtgataaaa
    30541 atatctttga tcccaccgct ggtttctgag ttttttacca atgaagtgat acaagccgaa
    30601 aagctaatga tcggcaataa aaacaagaaa gtatttaaaa tatgatacca taccgttata
    30661 tcccacgagg tgtggatcac taacagaacc attagaagta agaagccact gaacaacagc
    30721 agcgagtaac taatcaaact aaaggttact ttgcgattca agtttcgttt ggtaatgggc
    30781 gcactctggt ttcgtttact gatctctttg cgattaaatg ctagattgat gacgctgaaa
    30841 ccgctaaaaa tggatagaaa caacccataa gacaagaaat taaaggttgt gcccgtcaac
    30901 agctttttca tccgctgact atatcccgca cttagctcaa cttctccagt aaccgagagt
    30961 gccatttgtg tctgttccat agctgcatcg attgttttat cttcttcttg aaacagaatc
    31021 agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa ggcatctggg
    31081 cgactctgta tcttgatcgt cggcatcttt cctacttcga cttgttcggt aaagtctgca
    31141 ggcagatgta agataatgtt tacacgatta aaataaagag catcatcgat ttctttttgt
    31201 gaggttccat ccaatgtaac gactgtttcc ttgtcattca aatatttacg aaggccatcg
    31261 actacaggcg actcttcttc actaatcaag gcgattttcg catcttgcag agaggtattt
    31321 tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa cagtgataat tacacctatc
    31381 agtaaattcg atttatttgc tttaagcaat gtccaaatcg ttttataaag cgtcataacg
    31441 cacccctctt tccatccaag catttcctaa cgcaaaaagg aaaataaatc cgatcaacca
    31501 tagaagattg gtataaaaac tagccaaatc agtgtagaaa taaagtttat acaaactttc
    31561 gctgatcaag ttgacgagat ttaattgacc aacaatcgga atatgaagat caatccaata
    31621 cttcacttgt tccgacccca tcataccagc aaaaaagctc atcgcaatcg tcactgaaat
    31681 caagatgcta tccttttgca ccagattcat ttttggtatc aaattaccaa ccaatgtacc
    31741 taacaaaatc gcacacaacg accccagagc ggtaacaatc atcaaccatt gccaacgttc
    31801 accaaagtct acttgataga taaaacgaaa gacagctaac ataatataaa tctctgtcaa
    31861 aacaatcgta aagcttgctg ccaaattagc gattgaaact aacgattgct ttctaggaat
    31921 caaggacaag cgaataccat ttgacgattg atttgcttgc tgatcctgta cattgcggac
    31981 cccccacatc gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat aaaaactttt
    32041 gaaactcata ttacgacctg catgaacttc ttgaaaagat acttcttgtt caaaaacagt
    32101 catgatgggc gcgagatcaa cgttctccac ttctgccact ttactcgaaa ttataccaat
    32161 acgctgtaaa aattgatcca tgacgttttt catcaacgtc tgactcattc ctgcttgacc
    32221 cacatggagc gaaacagatg aaccatcaat gagataaaaa ccagccaaat catctgcatt
    32281 tagcttttca ttcgcttcta cttccgatag ttcctgtact tcaaataatg gtttatcatt
    32341 gacttcgact tgttctaaga ctgtggtaaa attttccgaa agcgctgttt gatccgcttt
    32401 gattacaatt ccagtattaa tcgtttcgag attgccacct tgatcaatct cgccaaaggc
    32461 cgccataaac atcaatccta aaagaattgg aaaggccaat gtccaaaaca acatggattt
    32521 atttcgcagc agtatttttg tgcgatattt ataaaaatgc caaaacatta tttcccctcc
    32581 tctagtctct caaatccttg ccagtaatct ctaaaaagac atcattcaag gacgcttctt
    32641 tggttgctat ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga atcggcaaca
    32701 atactgtatt tagttcagta gttgttactt ggagctgacc ttttttataa gcgattccat
    32761 cagaagctat tgttcgctga caacacgttt tctgggagta atgggatgtc gatcgtcaaa
    32821 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
    32881 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
    32941 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
    33001 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
    33061 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
    33121 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
    33181 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
    33241 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
    33301 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
    33361 agcatacagt tgatggcagt cgatttccca ctgccgtttg gtcctaacag acctaagact
    33421 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
    33481 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
    33541 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
    33601 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
    33661 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
    33721 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
    33781 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
    33841 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
    33901 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
    33961 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
    34021 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
    34081 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
    34141 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
    34201 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
    34261 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
    34321 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
    34381 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
    34441 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
    34501 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
    34561 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
    34621 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
    34681 accaagggag ggacaccaat gataacagta gtcattattg agatgatcca tttgtgacga
    34741 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    34801 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    34861 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    34921 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    34981 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    35041 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    35101 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    35161 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
    35221 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    35281 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    35341 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    35401 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    35461 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    35521 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    35581 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    35641 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    35701 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    35761 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    35821 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    35881 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    35941 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    36001 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    36061 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    36121 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    36181 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    36241 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    36301 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    36361 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    36421 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    36481 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    36541 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    36601 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    36661 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    36721 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    36781 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    36841 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    36901 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    36961 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    37021 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    37081 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    37141 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    37201 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    37261 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    37321 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    37381 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    37441 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    37501 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    37561 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    37621 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    37681 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    37741 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    37801 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    37861 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    37921 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    37981 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    38041 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    38101 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    38161 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    38221 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    38281 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    38341 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    38401 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    38461 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    38521 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    38581 cgaataaaag tgggccatct ttatgttggg caaccgcatc tgctacaaca gtttcgatac
    38641 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    38701 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    38761 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    38821 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    38881 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    38941 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    39001 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    39061 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    39121 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    39181 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    39241 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    39301 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    39361 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    39421 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    39481 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    39541 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    39601 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    39661 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    39721 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    39781 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    39841 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    39901 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    39961 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    40021 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    40081 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    40141 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    40201 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    40261 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    40321 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    40381 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    40441 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    40501 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    40561 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    40621 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    40681 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    40741 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    40801 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    40861 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    40921 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    40981 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    41041 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    41101 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    41161 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    41221 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    41281 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    41341 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    41401 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    41461 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    41521 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    41581 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    41641 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    41701 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    41761 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    41821 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    41881 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    41941 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    42001 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    42061 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    42121 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    42181 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    42241 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    42301 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    42361 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    42421 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    42481 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    42541 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    42601 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    42661 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    42721 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    42781 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    42841 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    42901 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    42961 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    43021 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    43081 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    43141 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    43201 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    43261 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    43321 aatccatgct ttgcaaaaag catcaaacca gatgtatccc gatctagtcg cgtcaccaca
    43381 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    43441 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    43501 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    43561 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    43621 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    43681 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    43741 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    43801 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    43861 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    43921 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    43981 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    44041 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    44101 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    44161 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    44221 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    44281 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    44341 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    44401 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    44461 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    44521 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    44581 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    44641 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    44701 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    44761 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    44821 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    44881 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    44941 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    45001 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    45061 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    45121 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    45181 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    45241 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    45301 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    45361 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    45421 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    45481 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    45541 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    45601 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    45661 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    45721 taatccaaac cggaaagctt acaacaaaac gggctgaatt ccctattgaa gaaggacttt
    45781 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    45841 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    45901 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    45961 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    46021 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    46081 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    46141 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    46201 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    46261 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    46321 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    46381 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    46441 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    46501 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    46561 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    46621 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    46681 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    46741 attaaaaaaa tacgtcgact cccttacaag agagccgacg tatttttttc gtttctattc
    46801 gagaaaatca aaaatagaaa gccttataaa cgatcgatca attcttctaa ttcatttaga
    46861 cgttgttcaa acatcgacat tgcatcttca atatatgcag cctgtgtcat atcaactcct
    46921 gcttttttca ttacttccac aggataatcg ctgttacctg cttttaggta agccaagtag
    46981 ttctctagtg cctctggttc ctggtttagt attttcttcg ccagtgctga agcagcagaa
    47041 aagccagtcg agtattggaa aacatagtaa ttataataaa aatgcggtat ccgtgaccat
    47101 tcgaatttaa tttctgggtc ttcttcgact gctggaccat aatattttgc attcaattta
    47161 ccataactat cacttaggta ttcactagtc aatggcacac ctttttcatc ttccgtatgc
    47221 atgaaatgct cgaattcagc gaattgtgtc tggcggaaaa ctgttccttt aaacccatcc
    47281 aagtaatggt tgagtacata agcccgtaca cgagggtctt tttctgtttc caataaatac
    47341 tccgttagga tattttcatt cgttgtcgaa gcaatttctg ctaaaaagat ggagtagtcg
    47401 ccgtacacat aaggctggtt cgaacgagtg aaataactat gaacactatg tcccatttcg
    47461 tggacaagcg taaatagctg atccagcgta tcatgccaat tcaataaaat atatggattt
    47521 gtgtcatagc ttcccgaaga ataagcatcg cttcgtttcc ctttattttc gacaacatcg
    47581 atccaacgtt cagagaatgc tttttctacg atggccatgt attcttcacc cattggtttc
    47641 agtgcttcta aagctttttc ttttgcttct tcgtacgtaa aggtaattgg agcttcccct
    47701 aagaccggtg tataaagatc atacatgtgc agtttttcca cttctaataa acgtttccgt
    47761 aattccatgt atcgatgtaa caaaggcaaa tgcttgttta ccacgtctac caaagtatcg
    47821 tatacacttt caggaatatg attattgctc aaagacgctt ctctggcaga gctgtaatta
    47881 cggactttcg ctttaaaatt atgtcctttt atatgtgtgc ctaaagtaga agcaaatgta
    47941 tttctaaatt gttcgtaaac actgtacaat cccttaaatg ctgcttcgcg cacccttcga
    48001 tccgtgcttt ctagcaactg accatacacg ccatgagata attggactat ttcaccattt
    48061 tctccttcaa tcgttggaaa aactagatct gcattattca aaacagcaaa tgtatcactt
    48121 gaagcatcaa agatttcacc tgctccagca agaagagatt cctgctcagc agataagaca
    48181 tgggctcgat tatctactat ttgctggata taatggcgat agacttccaa ttttggttct
    48241 tctttgaaat attgccaaat ctggtcatct gacaattgca atatttctgg ttcaaaccag
    48301 gaaacggctt cactaacttt cgaaaacaga ctgcttgctc ttgcataaag cgcttggtaa
    48361 tctgtatttc cagtgtcttg atcgttttta aggtgcgcat atacataaat gacttcagtt
    48421 tggcgataaa ccctcaatac gaattcaatc gcatttaaaa attgagaagc gccttgatca
    48481 agtgttcctt tgtgtttttc agattgtttt aactcttctg ataattccaa atatttctca
    48541 tcaaactctt ggtcgcttga aaagatcttg gtcaagtccc aagttaaatt ttcaggcaat
    48601 tcttctcgtt ttggcaactg ctttacttcc atgaaatcca ctccttttct tcccttagct
    48661 ctatctatca aaatagatca tagtctaaca tccacatgtc acagcatcta acttttctac
    48721 tcattcatgt gtctgcttcc atcaatttta gcataactaa atcttgtttt acatagaaaa
    48781 ccgattccta gcctattttt atacttacta ttttttcaaa gaagacaagt gtatgcattc
    48841 gttaaaaaac tgctgataga ccatctcttc atcaatcata ggaaaaagcc attccctttt
    48901 atgttctttt atttggcaaa gccatgagtt gaaatcagga actttctggt tttccaaggc
    48961 ttcttcgtac aacattcgat aaagaaaaac ctgttcttgt aaataaaaga aaaaccgact
    49021 tttttgatag atccacgatg atagatgcaa aagatgttta tgacgcaagt agcattgttc
    49081 ttgtatggat actgtattct tttgcttgtg aataagttgg cgtgtcagcc atttcttccc
    49141 atcttcactc actttttcta tttttttcat ttggttcatt ttaatttcgt tactagtgaa
    49201 tatttcttta aggtccctac tagaaaaagt ccagcagcta ctgcaaaagg aaatatgccc
    49261 tgatacagtc tctgtaatat gatgatacaa ataaagcttt tgtttcttga gatcagcttt
    49321 ccagcaatgc actcctcttt ttctattata gtagcagcaa cttttttcta tcaatgagaa
    49381 ttgcgattgt ccaagaaaag aattaccaag tatccaccat acttggtaac tgttattcaa
    49441 ataattttgg gttctttcaa taaatctctg gtgagacagt ctagaacatt ggatttctat
    49501 tgctagattc gccaacaaaa tatccggcct ttgccgcaac ctcggcaaat aggcttccaa
    49561 aaatactggt tctactgact gtcccagcca atccatgaag cattccttta actgtaagtg
    49621 ctcttttgat tctctttcac taaagaccga acagttatct gatttttgat gggcaaaatg
    49681 agcacatttt ttctttccct ttttcaaaat cactcttttt cgacagccac gacaataata
    49741 tttctttcct ttttctgcat gtttagcaaa acagcactta ttatattcat tcattgcaag
    49801 cagcattttt ctccctctga acttatttac gtctaagaat ttatttacac gaaaaaagag
    49861 ggcgtgaaaa agctattctg catcaagtaa taagaacagc taaacaaaaa tagttcccaa
    49921 tatttttcgt ttggttgggc ttgttaccgc agatgcaagc ttttgaacac atttatacga
    49981 aaaaaagact gcgacacaat ttttgtcaca gtctcttact ataatgctga tttatacaaa
    50041 ataaaatttc gttcgctcga tagcattacg cgccatcaga cattgtccat attcattcaa
    50101 tgtttctggt gtacgatcgc tcttttcagc aaactccaaa attcgagcaa tctgattctc
    50161 tacatccgct tggttgagat tttccatcca gaagtgaacg actaggtaat agcgctcttc
    50221 gaataaatac aaatcagtcc aagcagactc tagttcgaca tctgttgcaa gttggatcac
    50281 ttgttcaaaa tcttcaaaag caaaagttgc ttgtctttcc tgttcttcta gggcgcttcc
    50341 agataattct tcctccggat cgctgccaat aatctgctga cgaagaaagt cactcacatc
    50401 ttctgaatca acagtagaat cttctgattg agaaatttct tcatccggaa gatttttact
    50461 gataaacagt tctaatccgt ctccttttgg caaaacttgg aaagtgacag cttcgcttcc
    50521 tcggaactcc tcttcgatat caacttcttc caaaatacta taaaagaaat tttcgatttc
    50581 tttgtgattg ccaagtaaat ccaaaaatga aacgccccgt tcctctaaat cttcatgacc
    50641 aattagtaca cggatcgtat tttcattaat atgttccatt tccattttgc tacacctcac
    50701 tttgccacaa tgtttaatat agattcattg tagcgaaatc agcttaaatg taaagaaaaa
    50761 aaatctcgct gttaagcgaa tttaataaaa ccagcggatt tttcaacctt tcaagctatc
    50821 ttatttaact ataacatttt tggcagacga atactatata gccgactttt cagctaattt
    50881 tccctctcta tttttcgtgc aatcatcatc ttctactacc tttttataca tctaatcaag
    50941 aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc tttgtaatag tcttt
//
LOCUS       DAZUMO010000011        50843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000011 DAZUMO010000000
VERSION     DAZUMO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.011"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..418
                     /locus_tag="ACG8ER_000647"
     CDS             188..418
                     /locus_tag="ACG8ER_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS5049254.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            515..1816
                     /gene="murA"
                     /locus_tag="ACG8ER_000648"
     CDS             515..1816
                     /gene="murA"
                     /locus_tag="ACG8ER_000648"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5049255.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1829..2005
                     /locus_tag="ACG8ER_000649"
     CDS             1829..2005
                     /locus_tag="ACG8ER_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5049256.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2140..2436
                     /gene="yidD"
                     /locus_tag="ACG8ER_000650"
     CDS             2140..2436
                     /gene="yidD"
                     /locus_tag="ACG8ER_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS5049257.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2621..3088
                     /locus_tag="ACG8ER_000651"
     CDS             2621..3088
                     /locus_tag="ACG8ER_000651"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5049258.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3173..4057
                     /locus_tag="ACG8ER_000652"
     CDS             3173..4057
                     /locus_tag="ACG8ER_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5049259.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4171..4407
                     /locus_tag="ACG8ER_000653"
     CDS             4171..4407
                     /locus_tag="ACG8ER_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS5049260.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4495..4575
                     /locus_tag="ACG8ER_000654"
     tRNA            4495..4575
                     /locus_tag="ACG8ER_000654"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4529..4531,aa:Tyr,seq:gta)
     gene            4582..4653
                     /locus_tag="ACG8ER_000655"
     tRNA            4582..4653
                     /locus_tag="ACG8ER_000655"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Gln,seq:ttg)
     gene            4778..4948
                     /locus_tag="ACG8ER_000656"
     CDS             4778..4948
                     /locus_tag="ACG8ER_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049261.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5168..5875
                     /locus_tag="ACG8ER_000657"
     CDS             5168..5875
                     /locus_tag="ACG8ER_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5049262.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5934..6578)
                     /locus_tag="ACG8ER_000658"
     CDS             complement(5934..6578)
                     /locus_tag="ACG8ER_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS5049263.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6704..6991
                     /locus_tag="ACG8ER_000659"
     CDS             6704..6991
                     /locus_tag="ACG8ER_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049264.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7099..7419
                     /locus_tag="ACG8ER_000660"
     CDS             7099..7419
                     /locus_tag="ACG8ER_000660"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5049265.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7500..8814
                     /locus_tag="ACG8ER_000661"
                     /pseudo
     CDS             7500..8814
                     /locus_tag="ACG8ER_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            8811..9530
                     /locus_tag="ACG8ER_000662"
     CDS             8811..9530
                     /locus_tag="ACG8ER_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS5049266.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="ACG8ER_000663"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="ACG8ER_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS5049267.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="ACG8ER_000664"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="ACG8ER_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS5049268.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="ACG8ER_000665"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="ACG8ER_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS5049269.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="ACG8ER_000666"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="ACG8ER_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS5049270.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14976..15860
                     /gene="ftsX"
                     /locus_tag="ACG8ER_000667"
     CDS             14976..15860
                     /gene="ftsX"
                     /locus_tag="ACG8ER_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS5049271.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="ACG8ER_000668"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="ACG8ER_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5049272.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="ACG8ER_000669"
     CDS             17797..18654
                     /locus_tag="ACG8ER_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS5049273.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="ACG8ER_000670"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="ACG8ER_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS5049274.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="ACG8ER_000671"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="ACG8ER_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS5049275.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="ACG8ER_000672"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="ACG8ER_000672"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5049276.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /gene="pstB"
                     /locus_tag="ACG8ER_000673"
     CDS             21410..22168
                     /gene="pstB"
                     /locus_tag="ACG8ER_000673"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5049277.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="ACG8ER_000674"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="ACG8ER_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS5049278.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23435..24127
                     /locus_tag="ACG8ER_000675"
     CDS             23435..24127
                     /locus_tag="ACG8ER_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5049279.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24124..24891
                     /locus_tag="ACG8ER_000676"
     CDS             24124..24891
                     /locus_tag="ACG8ER_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5049280.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24991..26559
                     /gene="liaX"
                     /locus_tag="ACG8ER_000677"
     CDS             24991..26559
                     /gene="liaX"
                     /locus_tag="ACG8ER_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS5049281.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26856..27176
                     /locus_tag="ACG8ER_000678"
     CDS             26856..27176
                     /locus_tag="ACG8ER_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS5049282.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27176..27526
                     /locus_tag="ACG8ER_000679"
     CDS             27176..27526
                     /locus_tag="ACG8ER_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS5049283.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27693..28631
                     /gene="hprK"
                     /locus_tag="ACG8ER_000680"
     CDS             27693..28631
                     /gene="hprK"
                     /locus_tag="ACG8ER_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS5049284.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28646..29476
                     /gene="lgt"
                     /locus_tag="ACG8ER_000681"
     CDS             28646..29476
                     /gene="lgt"
                     /locus_tag="ACG8ER_000681"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5049285.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29520..30545
                     /locus_tag="ACG8ER_000682"
     CDS             29520..30545
                     /locus_tag="ACG8ER_000682"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5049286.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30560..31498
                     /gene="galU"
                     /locus_tag="ACG8ER_000683"
     CDS             30560..31498
                     /gene="galU"
                     /locus_tag="ACG8ER_000683"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5049287.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31623..32063
                     /locus_tag="ACG8ER_000684"
     CDS             31623..32063
                     /locus_tag="ACG8ER_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS5049288.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32066..32668
                     /locus_tag="ACG8ER_000685"
     CDS             32066..32668
                     /locus_tag="ACG8ER_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5049289.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32727..33833)
                     /locus_tag="ACG8ER_000686"
     CDS             complement(32727..33833)
                     /locus_tag="ACG8ER_000686"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS5049290.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34043..35044
                     /gene="ccpA"
                     /locus_tag="ACG8ER_000687"
     CDS             34043..35044
                     /gene="ccpA"
                     /locus_tag="ACG8ER_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS5049291.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35381..37726)
                     /locus_tag="ACG8ER_000688"
     CDS             complement(35381..37726)
                     /locus_tag="ACG8ER_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS5049292.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     RIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38005..38907
                     /locus_tag="ACG8ER_000689"
     CDS             38005..38907
                     /locus_tag="ACG8ER_000689"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS5049293.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39120..40895
                     /locus_tag="ACG8ER_000690"
     CDS             39120..40895
                     /locus_tag="ACG8ER_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS5049294.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40961..42547)
                     /locus_tag="ACG8ER_000691"
     CDS             complement(40961..42547)
                     /locus_tag="ACG8ER_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS5049295.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42798..43022
                     /locus_tag="ACG8ER_000692"
     CDS             42798..43022
                     /locus_tag="ACG8ER_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS5049296.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43164..44915
                     /locus_tag="ACG8ER_000693"
     CDS             43164..44915
                     /locus_tag="ACG8ER_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS5049297.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44915..46699
                     /locus_tag="ACG8ER_000694"
     CDS             44915..46699
                     /locus_tag="ACG8ER_000694"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049298.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47025..47876
                     /gene="licT"
                     /locus_tag="ACG8ER_000695"
     CDS             47025..47876
                     /gene="licT"
                     /locus_tag="ACG8ER_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5049299.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47969..49855
                     /locus_tag="ACG8ER_000696"
                     /pseudo
     CDS             47969..49855
                     /locus_tag="ACG8ER_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(49895..50797)
                     /locus_tag="ACG8ER_000697"
     CDS             complement(49895..50797)
                     /locus_tag="ACG8ER_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5049300.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        16468 a         8861 c        10823 g        14691 t
ORIGIN      
        1 tttggaataa gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag
       61 catttatttc ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct
      121 tttatacatt ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga
      181 cgtcattatg caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt
      241 tatctactta gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat
      301 gcataatacg caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc
      361 aagtaatttt ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat
      421 tttaaaaaaa ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag
      481 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
      541 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      601 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      661 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      721 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      781 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      841 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      901 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      961 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
     1021 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
     1081 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
     1141 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
     1201 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
     1261 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
     1321 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
     1381 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
     1441 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1501 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1561 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1621 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1681 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1741 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1801 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1861 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1921 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1981 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     2041 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     2101 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     2161 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     2221 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     2281 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     2341 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     2401 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     2461 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2521 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2581 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2641 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2701 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2761 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2821 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2881 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2941 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     3001 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     3061 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     3121 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     3181 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     3241 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     3301 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     3361 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     3421 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3481 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3541 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3601 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3661 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg
     3721 tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc
     3781 aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat
     3841 tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg
     3901 gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga
     3961 tttcgaaccg cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc
     4021 agcaaactat ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt
     4081 gcataaaaga agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct
     4141 gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa
     4201 gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta
     4261 tttataggga aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt
     4321 ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg
     4381 attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg
     4441 caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga
     4501 gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga
     4561 atccactctc ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc
     4621 cctcatgcgt tggttcgaat ccagctaccc cagtatacca caatcgtaag ctctcgttag
     4681 ttcgagagct cttttttttg ctttgctacc ttgttccttg aagattctgc tgatttaaga
     4741 tagagttaag ttaactacaa gaattggagg gatccacatg aaatggaaag aaaaagtaga
     4801 cgaagcagct gaaaagttat atgatttggt caaatcagaa aaatacaata ttgaagtgaa
     4861 tattccgaaa aaaggcggaa aagcggtccg ggtaaaatca aaacgcccaa cgaatcatac
     4921 aaaaaaatgg atggctaaaa accgctaata ataacaaaag atccataagt cggcattttc
     4981 atcgatttta gggtcttttg ttattttcta ggttggacat atctaaaaaa ttgtttctga
     5041 caataggagt aggaagaacg atgtgtgtct aaatcctctg ttgatggata gaaaccttta
     5101 tctttttcag ttgtgaatca ggtatatacc agttataata gaagaaacga aaggacgggt
     5161 gtacgttatg tcgaatcaat tacctgtata tatacagatt catgaccaat taaaaaatga
     5221 aatcgaacaa ggtatatgga aagtcggaga ccgtcttcct tcagaaagag aattagcagt
     5281 gaaattcaat gtcagccgta tgacgttgag acaagcaatt caaaacttgg cagatgaagg
     5341 aatcttagaa agaaagatcg gttcgggtac atatgttgca cgagaaaaag tacaagaaaa
     5401 aatgtctggt gcaacaagtt ttacagaaat tatggaatca caaaatcgag taccgtctag
     5461 tcggacaatc tcttatttct tgacttcgcc aagctcaagt gaaatggaaa aactaaactt
     5521 gagtaaagat gacacgatct tgcgaatgga acgtattcgt ttcgcagatg atgtccctat
     5581 ttgttttgaa gttgccagtg tgccacaaaa gctgatcgat ggctacagca aagcagagat
     5641 tactgaatcg ttgtatcgca cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa
     5701 tcaaacgatt actgctgtgt tggcttctga gaaaattgct gactatttag aggtaaaacg
     5761 aggagatgca gttcttcgtt tgcgccagat ttcttatttt gaagacggta cgccgtttga
     5821 gtatgtgcgt acgcaatacg taggaaatcg ttttgagttt tatttggaaa aataaaaaac
     5881 aggtgatccc tgattccgta ttagaaataa aagagaatca gaatcacctg tttttatata
     5941 tgatcagtta ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg
     6001 ttcagcagct caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag
     6061 atgacgctaa cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga
     6121 taattctgta attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag
     6181 gtaccttcta cgattccaat gtcagacaat gcttgagaga ccgcatgagt gtatgctcgt
     6241 attaatccgc ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca
     6301 ttgatcagtt gatttttttt gaggacttcc aacatcggta tgcccgccgt tccacttggt
     6361 tctccatcat cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag
     6421 ttatggttcg ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttcgtcc
     6481 tctgattcta ttcgtctaag agaacagata aagcgtgatt ttttgatctc gatctcactt
     6541 tgtccatttt cttttattgt acgatagcgc tcgatcatct tgtcacctct tttttcattt
     6601 tctttattca gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaattg
     6661 ctgtagtatg cgataataaa gacaaaggca ggtggatctg gggatgcagg aacgaatgaa
     6721 aaagtacgat gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac
     6781 ctttacccaa tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga
     6841 aagtacagat tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat
     6901 aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa
     6961 attagtgaaa tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa
     7021 tttctttctt tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt
     7081 taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg
     7141 agcacaagtt tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactct
     7201 gatattttcg ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agacgtggat
     7261 gtgttgttat taggcccaca agttcgcttt atgaaagctg attttgagaa acgtctggaa
     7321 ccaaaaggca ttcctttaga tgtcatcaat atggctgatt atggtatgat gaatggcgaa
     7381 aaagtgttag atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga
     7441 acaagcgatc caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata
     7501 tgaatcattt ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg
     7561 aggtaaattt accaaattat gagaagatcg cagcagtgga acgtttgagc aactggattc
     7621 tttgtcatcg ctgtggaatg agaacgtccg tcattgatgg acagcttgca gattacggtt
     7681 atttttgtac acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt
     7741 ttgatcagcc tgaagcaaaa cctcgagaag tcgtcttttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagcttc aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca
    13261 gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg
    13321 aagaaatcga attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag
    13381 aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt
    13441 tgttagatgg tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc
    13561 agcatgggta tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga
    13621 gtgtgaccct actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta
    15121 aattagctca agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctaatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcttttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacataaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgagaattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgcg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggactg ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta
    19321 aaggcagtgc cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggtcttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgattat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctattt
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 gattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagc ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcggaaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cggaagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 tgaagctgtg attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc
    22981 gtttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg
    23041 tttgtgacaa aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
    23101 aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca
    23161 gcatcaagcc gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga
    23221 ggatcatcaa ttgattacag gaaaactgat gaacacggat caaaaaggag aatcgattta
    23281 tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat
    23341 gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg
    23401 gcttttcttc ttcacaagta tcttcattgg cgccatgttg cttattggtg gaaaacaggg
    23461 tctatttgct ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat
    23521 ctttactaaa acagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg
    23581 gatgattatt agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc
    23641 agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg
    23701 gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt
    23761 ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt
    23821 cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg
    23881 gctttcagga aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt
    23941 cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt
    24001 caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt
    24061 aggaatagta ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa
    24121 acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc
    24181 ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg
    24241 ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta
    24301 ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct
    24361 tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt
    24421 ttagcaatcc aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact
    24481 ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct
    24541 gttttagatg cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg
    24601 caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg
    24661 tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg
    24721 tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa
    24781 tatacgatgg ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg
    24841 actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa
    24901 cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg
    24961 caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta
    25021 gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact
    25081 gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa
    25141 aaagatcagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag
    25201 ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat
    25261 gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc
    25321 gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa
    25381 gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa
    25441 atcaaagaat tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca
    25501 gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag
    25561 tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa
    25621 aaaatgtcag aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt
    25681 tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt
    25741 gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat
    25801 atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt
    25861 gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa
    25921 cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg
    25981 cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt
    26041 ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg
    26101 aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac
    26161 ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt
    26221 acagtgacat ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta
    26281 cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat
    26341 gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga
    26401 agtatcaaca gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac
    26461 caaatgttgc aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg
    26521 acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt
    26581 cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg
    26641 ccttattgcc gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa
    26701 ttatcgccga gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt
    26761 aatttgtgac cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat
    26821 tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat
    26881 ctaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg
    26941 atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa
    27001 ttattttata cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact
    27061 atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa
    27121 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
    27181 gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc
    27241 tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt
    27301 gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac
    27361 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt
    27421 gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat
    27481 gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc
    27541 gaactatcgg cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa
    27601 ttaaataaaa acgttttttt taattggtag tacttttggc tagacaatga taaaatgata
    27661 gagaacactc taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat
    27721 tagtgaaaaa attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca
    27781 tcgtaactgg ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt
    27841 cccataatcg tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc
    27901 cagaagagcg tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta
    27961 tttcaagagg attaccagct ccagaagaaa tgatccaggc agcaaatgaa aatgggatag
    28021 ctgtactgcg ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag
    28081 atggtcgcct tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag
    28141 gtgtactgat tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca
    28201 aacgcggcca tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa
    28261 cggtgatcgg agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga
    28321 tcattgatgt aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt
    28381 tggctgttta tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag
    28441 acacgaccgt tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag
    28501 gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg
    28561 gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag
    28621 gcaacgacta gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct
    28681 tgtagggatt cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat
    28741 gtggcttagc agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt
    28801 tatgttgtgg ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga
    28861 tcttccccag tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc
    28921 tatttatggc ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt
    28981 tatttctttg tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat
    29041 cggtcgttgg ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt
    29101 cttagaaagt ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta
    29161 tcgtcaacca acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt
    29221 gtttttacga aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat
    29281 gtggtatagt ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt
    29341 aggtgtgatc cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct
    29401 atttatttgg cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa
    29461 tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa
    29521 tgaattatag agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc
    29581 tcgccgaaaa cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa
    29641 tcaacaccca acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc
    29701 aagcattcac caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc
    29761 caacgaaagc aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag
    29821 tgatcatcca tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct
    29881 tgttagagga aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc
    29941 atgcagaaga agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa
    30001 aagcagcaaa atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc
    30061 ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg
    30121 gcgctattca cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat
    30181 tagctgagat cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat
    30241 taagcggaat aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg
    30301 caggcaactt gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg
    30361 tcgttgaagg tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg
    30421 aaatgcccat cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag
    30481 ccgcaaaaga aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt
    30541 tttgaaggag cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg
    30601 aacaagattt ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa
    30661 accaacaatc cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat
    30721 cgttacagga aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga
    30781 aacaaaccta agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga
    30841 tgtcaacttg catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca
    30901 agcaagagcc tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga
    30961 agacgaagta ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac
    31021 gatcgccgtt atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg
    31081 agaagtattg gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag
    31141 caaagcacca agtgatttag ctattattgg acgttactta ttgactccag aaatttttga
    31201 tgtattgtcc gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga
    31261 tacattgaat aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt
    31321 cggtgacaaa tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt
    31381 aggtgcacct ttacgtgagt acatcctgtc tttaggtaaa acattagaag cagaaggaag
    31441 caagatcgat cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc
    31501 cttttttcta acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat
    31561 ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga
    31621 tcatgtcagg aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg
    31681 tttttatcgt tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg
    31741 tagcagaagc aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac
    31801 aagtagaagg cttactggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg
    31861 caacgatcga tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta
    31921 atcattctag taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag
    31981 cgacggttgc cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata
    32041 ctgaaacgac aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt
    32101 agtcggaggc acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga
    32161 acttcgggat gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac
    32221 agatatggcg aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga
    32281 tctttctcaa caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc
    32341 agatgaagca ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga
    32401 cttatctaat cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg
    32461 aagtattgct aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa
    32521 agcaggagat actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa
    32581 agaaatgatg aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac
    32641 aatgacagat tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag
    32701 cggactagct ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta
    32761 gtcgtgaatg gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct
    32821 actttttcag gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact
    32881 aagtcatttc cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct
    32941 aaacggtgat tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga
    33001 tcaagttcac ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca
    33061 agagtgatgt tgtagatttt ttcttggaag tcggttggtt tttgataagc aattgtacga
    33121 gtagcatcac tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc
    33181 ggtgaaatct tcgtatttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg
    33241 actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcaatttct
    33301 gcaatgatcg cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca
    33361 acatcggccc atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt
    33421 tgtagacgtt ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca
    33481 gagatagcat cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc
    33541 tgaccatagc gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa
    33601 tagccacgga catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga
    33661 gtgaaaagga aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga
    33721 tcactttcgt atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg
    33781 acttggtgtt gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa
    33841 cctactttct aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa
    33901 cgttctaaaa actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat
    33961 tcttgagatt atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata
    34021 aaatatgaat aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag
    34081 agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa
    34141 accagcaaca agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc
    34201 agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt
    34261 cagcaatatg ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa
    34321 atacaatatt atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa
    34381 taacttactg gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga
    34441 aatccgtgga gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc
    34501 agatgaacaa gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa
    34561 caaattggca aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc
    34621 catcaatgga caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc
    34681 atatagcgaa ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt
    34741 cgatcggatc cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat
    34801 cggtattttg gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat
    34861 cacaagtaac aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca
    34921 accattatac gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga
    34981 agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa
    35041 ataataaaag gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg
    35101 agcaatttca aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt
    35161 ggcaggagaa caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt
    35221 tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa
    35281 tccgtgtata gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat
    35341 actattgtca caatccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg
    35401 attctttagt tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat
    35461 agttttcatc ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg
    35521 cccatagcga ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt
    35581 ttccaggtgt ttgaccagta aatccagata cttttttctt catcacatca gatgataatt
    35641 cgaatttctc atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat
    35701 tggcaagata attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt
    35761 gccctgccca gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt
    35821 attcattggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
    35881 ttgagatggc atctttgaat ggtgtagtga tttttgcatt gatcacacta cgcatcatgt
    35941 cattcatgat cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat
    36001 aaattacatt tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc
    36061 cgccgtttgc taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac
    36121 ctaaaggagc agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt
    36181 agtgctcgta agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat
    36241 aagccgggat attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc
    36301 cttcgttagt agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg
    36361 cataatctga tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa
    36421 caagaactgg tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt
    36481 gattctgact gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca
    36541 tcaatacatt tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat
    36601 tttcaacgcc attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat
    36661 atcctttatt ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat
    36721 cagacatatc agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat
    36781 tcatcacagt ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa
    36841 agtcttttgt aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt
    36901 cacgatacat actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt
    36961 ttagcgcgcc tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga
    37021 tataagcagc ttgcggcagc gtcacatctt ttgcatctac accaaagatt ctttttgcag
    37081 cttcctggac tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca
    37141 aatacgtagc aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga
    37201 tctcgtttgc tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt
    37261 gctgtgtcaa agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc
    37321 gtaaaacagc tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga
    37381 taattgcagt tttcaataaa ggtgaaatct tatcagatga aatagtcgtg cgcatcagat
    37441 ctgtctggat ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa
    37501 tgtttcctag ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat
    37561 aggcaaaata gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag
    37621 taataaaaac agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt
    37681 ctttttttct tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa
    37741 ctaatggttt tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt
    37801 ttttggcagg aatttctttt ttaaaagagt taaaacgtga gaaaatgatc tttattataa
    37861 aagagttctt aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga
    37921 aaaatcaatc attttctttg tatatcatct taccaatata taggaattgt tagtaacaat
    37981 tgcctttcaa ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag
    38041 aaaatgctgt taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc
    38101 tatacaggcg cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc
    38161 ggaaaagcgt acatggcaga aaaaggtcta acaaatatcg tcgttcatgc cccttatatc
    38221 attaatttgg ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga
    38281 gaagagattg ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct
    38341 catgtagggg caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt
    38401 ttgacaaaag aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa
    38461 atcggtcgaa catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa
    38521 ttatccgtta cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac
    38581 ttttcaggtg taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc
    38641 catatcaatg attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt
    38701 tttggaacaa tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat
    38761 atttcgaaaa tcttggaaac tccctatgta ggcaaagata aaaagaaagc ttacgcacca
    38821 tatggaaaag aaatcgccat gttcaaagcg caaacattcg atccagaagt gtttcctgag
    38881 ttattagcag aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca
    38941 ggcacattgc cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt
    39001 catcatgcat cttctccccc ctctttttaa atttagacaa acaaggtata ctggtagaaa
    39061 agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga
    39121 tgctgaacac tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc
    39181 ttggcatagc cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg
    39241 gaacagacat gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt
    39301 tggacggtaa atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca
    39361 gcttattttt agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt
    39421 tcagtatttt tgccagtata ttggttgcac tcgcgtttaa accgattgtg aagaacaagt
    39481 atcttctttt aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat
    39541 attccactcg aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt
    39601 gtttattagc tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt
    39661 tagggctttc tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc
    39721 ttccttttac tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact
    39781 tttctttcaa agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat
    39841 ggctgaaact ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcaatgga
    39901 tcgaatataa aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag
    39961 aatttggacg tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag
    40021 aaaacagtga gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta
    40081 cattccatac tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag
    40141 gtggaaaagt aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa
    40201 gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca
    40261 accaagaatt agaagaagcc tatcacaagg gaacaatcaa aaagaaaaaa gagttctatc
    40321 tgatgggatc aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc
    40381 aatcaggata cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt
    40441 tcggaacgca agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga
    40501 attggatcga tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag
    40561 tcgcgaatgt catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa
    40621 tagcagggat attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt
    40681 atagttcctc tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg
    40741 gtgtctcatg gttttgttct tatgcgccag aaaaaaagga ctatttttta agtgtctaca
    40801 ctggtgcagg cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta
    40861 aattaaatct gaaaaaagct ttatcttcta aataaaaaga cccttggtag ctagattttt
    40921 aacctaacta tcaagggtct ttgtgtggaa aaatcagttt ttattcgatt tcttctaccg
    40981 tttcctgcaa aagctgttca tattttttgt ttcctttata taaatcacca atcgtccatg
    41041 ccaacaggag aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt
    41101 gagcggaagt ggcttgctcg ccgaagaaac tttcgatctg tcctggtaat cctcgtaagt
    41161 tcaagtaagt tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt
    41221 taaatcggct acccatctcc gcttggctgt tggtcatcat cagtaaagga atcatcgaaa
    41281 acggcaacgc aaaagccaag aacacctggg attcattcat taagttattc aaagcggcat
    41341 gttcttcgat cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag
    41401 aaagcaatcg tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaatcctt
    41461 ccatgatgac ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa
    41521 gtgctacagc aaataaagtg gacaagatac cagattttgc tacagcaatc aaaataccat
    41581 tgctcatagc tgatggatca gataaagctt catacagacc aaaaaaggaa ggatcttgga
    41641 cagaaccggt tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag
    41701 ccattgacaa ctggatatta gaatcccaag tagaaaaacg tacagcttgt gctacatctt
    41761 cttcgttttg gtgatcgatc ttccgagttt gtgaaactgc tgaatgaaga tataagttat
    41821 gcggcatgac tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga
    41881 tgaccggtga gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc
    41941 aatctgggtt agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc
    42001 agacaaccag tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa
    42061 catctaatac agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg
    42121 cgattgctgc accaatgact tctgcaatat ctgttgccat aatggctaat tcagtcaaga
    42181 tccataaaat gacacctaat gtcttacttg tgcgtgctcg gattgcctgt gccaaatcca
    42241 tttggctcac gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag
    42301 acatcaaaat aactgacatc aataaatatt ggaaattttg tcctccggtg atcgaagtag
    42361 accagttacc cgggtccata tacccaactg ctactaacgc accaggtcca gagtaagcaa
    42421 gaagtgtttt aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta
    42481 aagataaacc atttgtatgt tcaataagtc gatgcttatg aaattgttta ttggattgat
    42541 tattcaagaa aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga
    42601 aaaaattaaa gaaaaagatt cggtacgcct aatttttttt catggcgcgt cttgctagat
    42661 gacgaaagaa attttgttct cctctaaaaa aaccactatt ttttgcatga aactaaagaa
    42721 tacgcctttc ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat
    42781 agaaaggaga gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt
    42841 tgctaatcgg cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag
    42901 aaaaaaatcc tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atggggcaaa
    42961 aaccatctga gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat
    43021 aggttctgac ccaacgactt tttacgggct gattttttca gtccgttttt tatttcgtaa
    43081 tttgttagac tattcagatt attcagcaaa aaagacaagc acagacaatc ctaggctctt
    43141 gataaagaca aaggaggagt aaaatgtcga tttttagaaa attaggctgg ttttttcgtc
    43201 aggaaaagaa acattattta attggggttt tctcgctatt cgcagtggcc ttggtacagt
    43261 tagttcctcc aaaagtgatt gggatcatca ttgatgagat cgccgataaa aatttatcaa
    43321 taaagatcat tttcgggtgg atcttcctat tgattatcgc tgctttttta caatatcttt
    43381 ttcgttatat ctggcggacg aatatttggg gaagtgcagc tcgattggag aaagaacttc
    43441 ggcagcagtt attcaaacat tttacagaaa tggacaatct cttttaccaa aaatatcgga
    43501 ctggggattt gatggctcac gcaacgaatg atttgaatgc gatccagaat gtagcaggag
    43561 ctggtatctt gacctttgca gattcagtga ttacgggcgg aacaacgatc attgcgatga
    43621 ttttgtttat tgattggcgc ttaacactga ttgcgctgat tcctctgcct ttgctggccg
    43681 tcacttcacg agtgctgggg tcaaaactcc atgatgcttt tcgtgattcg caagaagctt
    43741 tttctagtat caatgataag acacaagaaa gtatcactgg gatcaaagtc attaaaacat
    43801 ttggacaaga aaaagaagat ctagcggatt ttgaagaaaa gattgaagat gcaattatta
    43861 aaaacaaacg agtcaatttt ttagactcct tatttgatcc attcattacc ttgattatcg
    43921 gtctgtctta tgttgcaaca attattttag gtggaaagta tgtcttggaa ggcacgatct
    43981 cgatcgggca gatggtctca tttattagtt atatcggtat gcttgtttgg ccgatgtttg
    44041 cgattggtcg attgttcaac gtattggaaa gaggaaatgc ttcttatgat cgcgtcaatg
    44101 aacttttgca tgaaaagacg catatcgtgg aacaactggg tgcagtgcag accccggcaa
    44161 aaggcgagct gaatgtagct atttcctctt ttaattatcc gaaagaagaa tcaccagcat
    44221 tagaacaaat caagtttgcg atccaagaag gagaaacatt aggtattgtt gggaaaactg
    44281 gtgcgggaaa aacgacgatc atcaaattac tgatgcgtga atacgatgat tatcaaggga
    44341 aaatccaatt tggcggacgt tcgatcaaag aatattcatt gaatgcttta ttaggatcaa
    44401 tcggctatgt gcctcaggat cacttcttgt tttcgatgac cattcgagat aatatccgtt
    44461 tttctaatcc aaaactttca gaagaagaag tagagcatgc ggcttcttta gcatttatca
    44521 acaatgaaat caaggaatta ccaaaaggtt atgatacctt agttggagaa cgtggagtct
    44581 ctttatctgg cggacagaaa caaaggattt cgattgctcg agcattgatc aaaaatcctg
    44641 aattattaat attagatgat gcactctctg cagtagatgc caaaacagaa gaagctatct
    44701 tgcataattt aaaagaaata agaaaagaga aaacaactat cattgcagct caccgtttaa
    44761 gtagcgtgat gcatgcaaaa gaaatcattg ttgtcgatca agggaaaatc attgaacgag
    44821 gaacccatca tgagctgctt gcactaggcg gctggtatga acaaatgtgg gagaaacagc
    44881 aattagaagc gaagatagaa ggaggcgagg cctgatggat gaaaattatc aatcggaatg
    44941 gacaaaatcg attccactta aagaacagaa aaagatcatc aaacgactga tgaagttcgc
    45001 caaacctttt agaagaacgt tcatcgttgc tatcttattt gcgtttgctt tgtctgtgat
    45061 caatgtccta ttgccaagga tcatccaaac ttttatggat gatcatctgg ctaaacagtc
    45121 ggctacgaca caagttattt tgttttttgc tggtatgtac ttatttggag tcatagtaaa
    45181 aagtatcatt tggtttttcc aatggtattt gtattccatg gcctcattga agacctatca
    45241 gtatattagg gtaaaattat ttgaaaagct gcatacacta ggtatgcgtt attttgatca
    45301 gacaccggca ggatcaaccg tttctcgagt gacgaatgac acagaaacgc tatttgaatt
    45361 ttggtacgtc tttttgatgg tattgactgg gatctttgca gtcgtttctt cattccttgc
    45421 catgtttcaa atcaatggag aaattgcctt gtggactttg gtgtttttgc cgattttagg
    45481 aatcgtcatc tggtattacc agaaattcag ttctcgtatt taccgaaata tgcgggaaaa
    45541 attaagtcag ctgaacacga aattgaatga atcgatttcc ggcatgcgta ttatccagca
    45601 gtttcgccaa gaagctcgtt tagctaaaga atttgaacaa gtgaacaatg agtatttaga
    45661 cacgcgttat gcgatgatca aaacgaattc gattctgtta agtccgatca tcaattttct
    45721 atatgcattg gcgattgcgt tggtgttgac tttgtttggg atcgatgcgt tgcattctcc
    45781 cgtagaagtt ggactgatct atgcatttac gacctatgtc cagggatttt ttaatccaat
    45841 gtctcaaatg atggactttt tgagcatctt cacagatggg atggttgctg gtagtcgtat
    45901 cttgaagatt atggatacgg aagaatatgc accacaacaa aaccctggag cagatcaaat
    45961 gattacttca ggtaagattg agtttcgcaa tgtaagcttt tcttatgatg gagagcacaa
    46021 tgtattaaac aatatctctt ttgttgctaa tccgggagag accgttgcat tagtgggaca
    46081 caccggaagc ggaaaaagct cgatcatcaa cgtattgatg cggttttacg aattttatga
    46141 aggtcagata ttgattgatg atcatgacat tcgagaatat ccaatggaag agttacgtaa
    46201 gaaaatgggg ctggtcttgc aagatgcttt cttgttttat ggggatatcg caggaaatat
    46261 tcggatgatg aatccagcta tcacagacga acagatacgc catgcggctg agtttgttca
    46321 agcaaacaaa ttcatcgatg aattaccaaa aggatatcac tcaaaagtga tcgaacatgg
    46381 tgccagctat tctagtggac aacgacagct gatcactttt gcgcgaacaa tcgtgactga
    46441 tcctaaaatt ctagtattgg atgaagcaac agcaaatatc gatactgaaa cagaaggatt
    46501 gatccaagaa ggattggcaa aaatgcgtca aggacggaca acgattgcga ttgcccatcg
    46561 gctgtcaacg attcgagatg cagaattgat tttagtttta gacaaaggtc atatagtaga
    46621 acgcgggaat catgaaacac ttctagcgca aaatggtttg tatgcagata tgtacaaact
    46681 gcaaagcagt ggtatataaa agatgttgtg acagtagaat tttgacctaa gcactagagt
    46741 gaaaataaaa catagcttct catatttttc atctgtgaga gttagtgtcg tagtcaccag
    46801 atttaaaaaa agattcgaca gctagtcaga agtgcctaat agccactaag aaagcgttga
    46861 caaaagcatt tgctttcgat aatataaaga aaacgataca acttggatag tgattgcctg
    46921 atgcaagcta aacctgatga gtctcccatg gtttcatgga cggctttttg ggtttttctt
    46981 tttgttttgg atacagccct atacatcatg gaaaggagca aaacatgctg attgagaaag
    47041 tactgaataa caacgtcgta ataaccaggg atgaaagaaa tcaggaaatg atcgtgatgg
    47101 gaaaaggttt ggcattcaaa aagaaagtgg gtgacccgat ttttgactca tgcatcgaca
    47161 aaaaatttca gttgacaaat gacgggcttt cacaacagtt ccaagaattg gcacaagcga
    47221 ttcctcttga gtacttagaa atttcttgtg aaatcattga atatacaaaa gaggctctac
    47281 aagtccagtt gaatgattct gtgtatattg cattgacgga tcatttgtat acggctatcg
    47341 aacgtcacaa aatgggaata tttgtaccaa atgtattgct gtatgatatc aaacaactat
    47401 ttcctaaaga gtatgccatc gggaaaaaaa cagtgaagaa aatctcggat aaataccaca
    47461 tcaagttatc agaagatgaa gccggattta tcgcattgca tttagtcaat gcacaaggca
    47521 ttcaaagcca gcaagacaat acctacctgt ttactataac aatacaggat attctaaata
    47581 tcgtccgcta ttactataag attacatttg atgaagattc agtccatttt tttcgattcg
    47641 tcacacactt acgttttttt ctgacacgcg tcctagatgg agaaaaatca gaagtgggag
    47701 aagtttctga ggaattgttt caggttattc aaaaaaaata taaggatacg tttggttgcg
    47761 ttgagaaaat agcagacttt ttagccgaga cgttcaacta ccagatgtcc acggacgaaa
    47821 aaatgtattt aacgatccat actgcgcgtt tagtgcatga gaatcgttgt aaataaaaaa
    47881 aggaggataa attaacaaga acgtatgaaa taagggtagt gacattcagt tggctaaacc
    47941 ttccactaat aaattggagg gaatagaaat gggaaaatac caagaattag cgagaaaaat
    48001 cgtagaaaat gtcggcggga aggaaaatat caatggtttg acacattgta tcacaaggct
    48061 tcgtttcaaa ttaaaaaacg aagaaaaagc aaatgatgag atcttgaaaa atatggacgg
    48121 tattgtaacg gtaatgcgag cgggagggca gtatcaagtt gtcattggga atcatgtgcc
    48181 agtcgtgttt gaagaggtta taaaagcagg aaaccttact tttgatgagg ctgtttcaac
    48241 agaaaaaatg agaccgtttg atatgcttat tgatggaata tcgggctgtt tccagccatt
    48301 ccttgctatc ctagctgctg gcgggatgat tcgtggactg acagcattcc ttgtcttctt
    48361 aggcgcgttt gaccgcggtt ctggcacatt tgttatgttt gataatatcg gtgattctgt
    48421 attccaattt atgcctgtta ttatcggttt aacagctgcc cgtaaattta aagtaaatga
    48481 attcgtaggg atgttgcttg gcgcagcatt aatgaatcct agcttgtcgc tggaagcttt
    48541 gagtggtgca gcagaagcac cactaacgac catattttct ggcactattt ttgaagcacc
    48601 tatttatcaa acagtattcg gcattccctg gattgcgcgt aactacgctt cttctgtcat
    48661 tcccattatc tttattgtgt tgttagctag ccaaattcag aaaccaatta aaaagttagt
    48721 acctgagatg atcgcgaatt tcttcgttcc attcttcacc gttcttatta caatgccttt
    48781 aggtttttta ctcgttggac cagtattcac attcgcaact gatattttaa tggctggatt
    48841 tgaaacactt ttagcacttt cgccagttat ttatggagca atcgtaggct tcttctggca
    48901 aattctggtg atgtttggtc tccattgggc aatcgtacca atgggattga tgcaattttc
    48961 tgtgaatggt tggcaaaata ttatgacacc tgttgcagta gtgtcttttg gacaaactgc
    49021 agcacttacc gcactctact ttaagcttag aaatccaaaa gacaaagcaa ttgcaatacc
    49081 agccattgtt tctggtattg tggggattac agagccagct atttatgggt ttacattacc
    49141 acgtaaaaaa atatttattt ttacctgtgt cggtggtgcg ttagggggcg cgtatagtgg
    49201 gttaatgaat ttaacttctt ggaatcaagg tggactaggc atctttacaa ttcccaacta
    49261 tattagacca gatggtgatt taacagatgt gattaatgta ttgattggga tcgccattgc
    49321 gatggtggta tcctttacgc tgactttttt cttttggaag gatgaagcag gtgaaacaga
    49381 tgatatacag aaaaaatcag gtaaagaaat tgtaaaaaca ccgatacaag gacaaatcgc
    49441 tccactaaat gcagcaaagg atgcagcttt tgcacaagga acactaggtc gaggaatctt
    49501 gatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtca tgaccctgtt
    49561 cccgacgaaa caggcaattg gaatggtttc tgaaacaggg ctggaattgc tgattcatgt
    49621 cggattggat actgtgcagt tggaagggaa atattttgaa agcttagtgc agcaaggata
    49681 aagagtgaaa caaggcgatc tgttgctacg cttcgacaaa aagaaaatcg aaaaggaagg
    49741 atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg atattttgga
    49801 aaaccaaggg gagacggtcg ggccagacga tgcacttatc accgcactga cctgaacggt
    49861 taggtgaaga atggactagg cttaactatt ctttttattc cacttcttta ttttcaaaat
    49921 tgaaaggctc tttcatgatg gctgagctga tagggcgttt atcgaatttt tgaccgacag
    49981 aaattagatg gactaaattc tgttgataat tttctgcaaa aacacaacta tataacacgt
    50041 ctagaagata acaaattgat gtattgatcg taaaattacc aatttttgaa tacagtcgct
    50101 cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgcaga tagcgttgaa tcaccgatac
    50161 tggtcaaagc taaaattgga acatgatttt gtttcaatat ttctgcggta cgaaggatca
    50221 cattgttttc acccgtataa gaaatcagaa tcccgcacat atcactcgtg caattgtacg
    50281 cttcatagaa tgattcacca aaagtggtga caatcgtgac atccattttg atgcgcttca
    50341 ttttcagcat gaaatcttga gaaatcaaag tattcgagtt actggcgaaa atcacgattt
    50401 tttttgcttg aagcattagt tgtttggctt tttgcagttg gtcatgatga atgagagaaa
    50461 gtgtatcatg aatcgtcatc tgttctaaag aagccatttt atttgcgatc gtcataatac
    50521 cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg aaaattccct tcaagatagg
    50581 ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc attttttttg
    50641 ctacacggat gagtgtagag gggtggacat aacatcgctc agcaatcgct tggatggtca
    50701 tttcgccaag tgctggagaa tcgcttaaga gaaactggat cacttcattt tctgaaggag
    50761 aaaaggagat ttctttgatt ttttcacgta ataacatgta gataccttct tcttattttt
    50821 ttacgtaaat tataactttt ttg
//
LOCUS       DAZUMO010000012        50120 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000012 DAZUMO010000000
VERSION     DAZUMO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.012"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..890
                     /gene="pnuC"
                     /locus_tag="ACG8ER_000698"
     CDS             141..890
                     /gene="pnuC"
                     /locus_tag="ACG8ER_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS5049301.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(1074..2351)
                     /locus_tag="ACG8ER_000699"
     CDS             complement(1074..2351)
                     /locus_tag="ACG8ER_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049302.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(2430..3074)
                     /gene="pcp"
                     /locus_tag="ACG8ER_000700"
     CDS             complement(2430..3074)
                     /gene="pcp"
                     /locus_tag="ACG8ER_000700"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS5049303.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(3273..4217)
                     /locus_tag="ACG8ER_000701"
     CDS             complement(3273..4217)
                     /locus_tag="ACG8ER_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS5049304.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(4219..4905)
                     /locus_tag="ACG8ER_000702"
     CDS             complement(4219..4905)
                     /locus_tag="ACG8ER_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS5049305.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            5379..5699
                     /locus_tag="ACG8ER_000703"
     CDS             5379..5699
                     /locus_tag="ACG8ER_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5049306.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            5853..6758
                     /locus_tag="ACG8ER_000704"
     CDS             5853..6758
                     /locus_tag="ACG8ER_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049307.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            6755..7528
                     /locus_tag="ACG8ER_000705"
     CDS             6755..7528
                     /locus_tag="ACG8ER_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5049308.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7709..9133)
                     /locus_tag="ACG8ER_000706"
     CDS             complement(7709..9133)
                     /locus_tag="ACG8ER_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5049309.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            9344..9790
                     /gene="nrdI"
                     /locus_tag="ACG8ER_000707"
     CDS             9344..9790
                     /gene="nrdI"
                     /locus_tag="ACG8ER_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5049310.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(9855..11837)
                     /locus_tag="ACG8ER_000708"
     CDS             complement(9855..11837)
                     /locus_tag="ACG8ER_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5049311.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11851..12747)
                     /gene="murQ"
                     /locus_tag="ACG8ER_000709"
     CDS             complement(11851..12747)
                     /gene="murQ"
                     /locus_tag="ACG8ER_000709"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5049312.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(12750..13811)
                     /locus_tag="ACG8ER_000710"
     CDS             complement(12750..13811)
                     /locus_tag="ACG8ER_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5049313.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(14022..14549)
                     /locus_tag="ACG8ER_000711"
     CDS             complement(14022..14549)
                     /locus_tag="ACG8ER_000711"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5049314.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(14616..16118)
                     /gene="eat(A)"
                     /locus_tag="ACG8ER_000712"
     CDS             complement(14616..16118)
                     /gene="eat(A)"
                     /locus_tag="ACG8ER_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5049315.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(16376..17496)
                     /locus_tag="ACG8ER_000713"
                     /pseudo
     CDS             complement(16376..17496)
                     /locus_tag="ACG8ER_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            17622..18563
                     /locus_tag="ACG8ER_000714"
     CDS             17622..18563
                     /locus_tag="ACG8ER_000714"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5049316.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(18713..19609)
                     /locus_tag="ACG8ER_000715"
     CDS             complement(18713..19609)
                     /locus_tag="ACG8ER_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5049317.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            19878..21218
                     /locus_tag="ACG8ER_000716"
     CDS             19878..21218
                     /locus_tag="ACG8ER_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5049318.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            21436..21879
                     /locus_tag="ACG8ER_000717"
     CDS             21436..21879
                     /locus_tag="ACG8ER_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS5049319.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(22383..23801)
                     /gene="nhaC"
                     /locus_tag="ACG8ER_000718"
     CDS             complement(22383..23801)
                     /gene="nhaC"
                     /locus_tag="ACG8ER_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5049320.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(24204..25427)
                     /locus_tag="ACG8ER_000719"
     CDS             complement(24204..25427)
                     /locus_tag="ACG8ER_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5049321.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(25458..26297)
                     /locus_tag="ACG8ER_000720"
     CDS             complement(25458..26297)
                     /locus_tag="ACG8ER_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5049322.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(26314..26994)
                     /locus_tag="ACG8ER_000721"
     CDS             complement(26314..26994)
                     /locus_tag="ACG8ER_000721"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5049323.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(26996..28069)
                     /locus_tag="ACG8ER_000722"
     CDS             complement(26996..28069)
                     /locus_tag="ACG8ER_000722"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5049324.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(28125..28363)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(28406..28639)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28716..29387)
                     /locus_tag="ACG8ER_000723"
     CDS             complement(28716..29387)
                     /locus_tag="ACG8ER_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5049325.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(29474..32122)
                     /gene="mgtA"
                     /locus_tag="ACG8ER_000724"
     CDS             complement(29474..32122)
                     /gene="mgtA"
                     /locus_tag="ACG8ER_000724"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS5049326.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(32464..32638)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            32811..33248
                     /gene="ndk"
                     /locus_tag="ACG8ER_000725"
     CDS             32811..33248
                     /gene="ndk"
                     /locus_tag="ACG8ER_000725"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS5049327.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(33270..34136)
                     /locus_tag="ACG8ER_000726"
     CDS             complement(33270..34136)
                     /locus_tag="ACG8ER_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5049328.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(34296..36389)
                     /locus_tag="ACG8ER_000727"
     CDS             complement(34296..36389)
                     /locus_tag="ACG8ER_000727"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5049329.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(37138..37761)
                     /locus_tag="ACG8ER_000728"
     CDS             complement(37138..37761)
                     /locus_tag="ACG8ER_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049330.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHVGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(37920..39317)
                     /locus_tag="ACG8ER_000729"
     CDS             complement(37920..39317)
                     /locus_tag="ACG8ER_000729"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5049331.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(39349..40320)
                     /locus_tag="ACG8ER_000730"
     CDS             complement(39349..40320)
                     /locus_tag="ACG8ER_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5049332.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            40694..41119
                     /locus_tag="ACG8ER_000731"
     CDS             40694..41119
                     /locus_tag="ACG8ER_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049333.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(41607..42308)
                     /locus_tag="ACG8ER_000732"
     CDS             complement(41607..42308)
                     /locus_tag="ACG8ER_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5049334.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(42324..42764)
                     /locus_tag="ACG8ER_000733"
     CDS             complement(42324..42764)
                     /locus_tag="ACG8ER_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5049335.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(42764..43339)
                     /locus_tag="ACG8ER_000734"
     CDS             complement(42764..43339)
                     /locus_tag="ACG8ER_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS5049336.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            43580..43789
                     /locus_tag="ACG8ER_000735"
     CDS             43580..43789
                     /locus_tag="ACG8ER_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS5049337.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(44187..44699)
                     /locus_tag="ACG8ER_000736"
                     /pseudo
     CDS             complement(44187..44699)
                     /locus_tag="ACG8ER_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(44825..45526)
                     /locus_tag="ACG8ER_000737"
     CDS             complement(44825..45526)
                     /locus_tag="ACG8ER_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5049338.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(45634..47077)
                     /locus_tag="ACG8ER_000738"
                     /pseudo
     CDS             complement(45634..47077)
                     /locus_tag="ACG8ER_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(47091..48956)
                     /locus_tag="ACG8ER_000739"
     CDS             complement(47091..48956)
                     /locus_tag="ACG8ER_000739"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5049339.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(49077..49928)
                     /gene="licT"
                     /locus_tag="ACG8ER_000740"
     CDS             complement(49077..49928)
                     /gene="licT"
                     /locus_tag="ACG8ER_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5049340.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT        14487 a        10568 c         8677 g        16388 t
ORIGIN      
        1 tcaagaaaac aaacagaaaa ttatcataac acaaattatt tataattgaa agactagttt
       61 tttttcaaga aaagcataca atagattgtg ttagtaaata cagaacacac tacatttagt
      121 gtcttaagga gtcctcatac atgaagtcat attttcagtt tttagctcat cagctaaaag
      181 gctggccgca acaaaattat tatcttttct ttttcagtct tggctgccag gtcatgactc
      241 ttgtcaatag tcccatcact tggctgtccg ttattacatt tatcggtacc acgcttggtg
      301 tattgtgtat tttatccatc aatgcggcaa aatcagtcaa cggtatttta ggtattttgt
      361 ctgcgatttg ttttattatc gttgggtttt cagcaaagaa ctatttaagt atcggtgaac
      421 agattgctta tatgatcaca ttggatatcc ccgtattgtt aagtaaagaa tggaatcata
      481 atatggcttc aaaaatccga aaattcactg gaaaaagctg gtttatcgca atcatcagta
      541 ctttccttgt ctatcttgtt tcaggattcc tgattggtcg ttatacagat gatcctcggc
      601 cgtgggtaga tgccattgct ttttctatta gcttgactgc tggtatcatc agtttcttgc
      661 gttacaacaa ccaatattac tggtggttag cttcagggct tgctcaaatg gtcctttggt
      721 atatttcatt tacgcatgga tctgcaagtt tggcgatgtt cgtgaacagc tctgtttact
      781 tgctgaatga tgtccttgca tttaccgttt caccttggta taaccaaaaa gaacgtgaac
      841 gtatgcaaaa aatggaagaa gaatactaca acgcacaaca acacgcataa aaaaactgag
      901 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa
      961 tcagaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg
     1021 tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctctat tttttatagt
     1081 aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc taacttctga
     1141 taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc tttccatttg
     1201 tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg ccatttttct
     1261 tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc tttacgcaat
     1321 acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc ttctgtaaaa
     1381 ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat gaatcgttgg
     1441 tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg ttttgtcttt
     1501 ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac ttcttcatat
     1561 acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa ttcttctaca
     1621 tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg tcgctttttt
     1681 gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc ttgataatcc
     1741 gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc taattttata
     1801 tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca gtatttttgc
     1861 catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc gtctaaggcc
     1921 agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga gacatgcagg
     1981 tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg cgatttaacg
     2041 atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg gcttcgtttt
     2101 gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc agctaaataa
     2161 tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac tagttcccgc
     2221 tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta ttgtttagaa
     2281 attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa gacggattgc
     2341 cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac taacataacg
     2401 ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt tccggaaata
     2461 tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc aatgtcttct
     2521 aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga gaatggcacg
     2581 tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag caaacgatac
     2641 atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg tagaccatga
     2701 tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc ggctggaccg
     2761 ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat tcttgcttct
     2821 gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc ttgtccgaca
     2881 cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg actgcttttt
     2941 tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc atctggcatt
     3001 ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc aaaacctgtt
     3061 actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt tatcttttat
     3121 cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata acataacaga
     3181 tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag aagaggttgc
     3241 gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat ataagcaatt
     3301 tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc gtatttgtct
     3361 ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg tgtcagcagg
     3421 gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt cgctccatga
     3481 ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc aaatgcattc
     3541 cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc gatgttcaca
     3601 tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac tccagcgctt
     3661 gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg ccctactgtg
     3721 cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg agaaaagaac
     3781 atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc acctaaattg
     3841 gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat aaaaattttc
     3901 attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg gctttctcct
     3961 ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc accgttcacg
     4021 attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca gaaaaaggct
     4081 gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt gtaaatcaat
     4141 acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa aatatcagga
     4201 tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg ctaatttctt
     4261 ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca ctggaacttc
     4321 cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg ttgattggac
     4381 tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc agacattttc
     4441 catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt ctaacccttt
     4501 tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc cagcaactcc
     4561 accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa tatctacaat
     4621 cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt ctttcagacc
     4681 atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca tcccgcgatt
     4741 gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac cgccagtcaa
     4801 agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa atgcaaaccc
     4861 gagaataaca atcaagacgc ctaaaaggct aagataattt tccatatatt ttcctccata
     4921 aaatgacaat atttttttaa ttataaagca aaacacataa aaaatatagt tgttttctca
     4981 cgaaatagac actattttta aatatttttc gttatttttt tatcacaaaa attatcttac
     5041 ttattgtaag gtaaagcttt ttttaattta ttgcccgata atatatagaa ataaggtcta
     5101 gttactgttt tttttaaatg attaataaat aacaacttct ttctatgaaa ttgcaaagca
     5161 ttcatcttct tttccgctaa cgatatatcc gtcagtgctt ggtacacttt actaaaaagt
     5221 atgagattgc cgatatctgg ataggtgtgc tatactacag aaaaagatat acaatctgcc
     5281 accgggtagg ataaagagga tgatcattcc attaggtctt tgaagggatg attttcctct
     5341 ttttgtgtat ttttttatag ttaaggagga atgtaagtat ggcatggatg gaattaatca
     5401 tagcaggaat tttggaagta ttctggtcaa cgatgatgaa atggagtgat ggtttttcta
     5461 aaatcaatta ttctttttac acagtgattg gcatgattgc tagtttttac tttttgtcaa
     5521 aggcaatcaa aagtttgccg atgagtctct cttatcctat ctggacaggg attggcgcag
     5581 ttggttcggt cataattggt gtggtcttgt ttcatgataa gctgaatctt tccacttggt
     5641 tctttgtggg gatgctactc atcagtatca ttggcatcaa gattactagc ggccactaga
     5701 cacttggaat aagtactgat cagaaaaaat aagccttttt atttaatatt caattttttt
     5761 atgaaaaaat aagaaataag tccaatgacc tagcgacttc tccactcatc aggtgtatgc
     5821 ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc caagaattat
     5881 ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta tcccctggca
     5941 aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg aaagctattt
     6001 taggactgat ccattactca gatggaacaa tctgtattgg tgaaaatgag atttctccct
     6061 ctagtcataa agggttggaa caagtaggag cattgatcga atacccgggt atctatccat
     6121 tcttaacagg ctatgatcat ctgaagttat tctctgaaac caacgatgta tctgctatcg
     6181 acactatcgt gaatcagctc aagatggaaa agtacattca caaaaaagcg aaatcctact
     6241 cgttgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat ccgtctatcg
     6301 ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat gtgcgaaaaa
     6361 tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac ttactcagcg
     6421 agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaagatc atcaaacaat
     6481 gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct acaacaaatg
     6541 atctagctgc tcgaacgatc ttgacagaag ctggatatac tttggaagat cctgataaaa
     6601 tcaagctttt aaaagaaaac gaagaattat taggaaccgt gatccagcta ttgtctgcac
     6661 aaaatatcca tgtgacagac gtccagcatc tgacgaatga tttggaatct tctctattgg
     6721 aaattctgga ttcagtaaag ggggaagaag aaaaatgaca ttgcttgtaa aacaagaatt
     6781 atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag ttgttttgct
     6841 cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag ttgaaatgac
     6901 tgctcagctt tttagtgcca catcttggat tgtctttatc atgattgcag ctgcaagcac
     6961 gatcatttcg atggaagcac aatatggtac attgaaaaat ctcctttatc gaaagtattc
     7021 tcgaggagaa atattagtaa gcaaatggat cacattagtt atttattctg tttatttata
     7081 ccttttagct attattgtca ctgtgttgat gaaactgatt cttttccctt cgatttcctt
     7141 cacggagaag gtttctactg gtcaaacact gatccaaagc ttgttcactt atactttggg
     7201 tagttatatc ggtttatggc tgattttaag tttagtgttg atgattgctt gctttatcaa
     7261 tagttccggc gcatccatct cagcaggaat cgtcttttat tttgcttcgt caatcatatc
     7321 tggtatcctt attgcactca tccaaaaatg ggagtggatc aaatggaatc cgatcagtat
     7381 gttgaatctg caaaaccaaa ttggaaacga agagatcatg aagcaattga ctcatctttc
     7441 tactaatcaa atgctattcg gtaatttagc ttacatcgtt ttgttcttag cattaggata
     7501 tctcgtattt aagcgaaaaa atgtatgatg actcatcaaa aaagaggctg tgaaagagac
     7561 gttcagcctt attgccgatt ggctgtctct taaacaccgt ttattctgaa aaaggagctg
     7621 tgacaacatt tgtgtcacag ctcctttcct agtatgtttt tgaataaacg acaggctcac
     7681 aacctctctt tatacttctt ttatacttct atacgtgaag catcccagtg gcaaataaaa
     7741 ccattgcaat cactccgacc atcgtcacac ctataagtaa aatcttatct aagtggatat
     7801 gttgtacagg ctcgttttgc tccttacgtg ctttgaggta aaaataaatg cctggcaaga
     7861 aaagaacaag aacaagcatt acttcattaa aggctacgac tagcatagcc catacttgaa
     7921 aggcacttgc taaaagccct actaaaaatt ggacccattc tttccgctga taagaaagtt
     7981 tcatttggta caatcctaag aaaagccacg tgaagaaaat agctgctgta ccaagtgtgt
     8041 aagcaaagtt ataagcactg tcagtgaata ataatgaaag taaaaagaca ttcgtacata
     8101 acgtcgtcaa tacgatagca taagttggtg ccccattttt gttgagtttt ccccatgctt
     8161 taggcaaaag tccgtcttca gccatgtttc ccattgtttc aatcggtaag atcgtccatg
     8221 agagccaaac accaactaaa gaaatgatca aaccgccgtt gatcacgact gctccccatt
     8281 taccaaccat catttcaaac acatagccca tagcaggctg cgccttgatc gtttgcagct
     8341 gattcatcgt cattgtccca tatggaagca tggaaaccaa gacgtaaata ataatcaagc
     8401 cgaatatccc tagaatcgtt gctttcccaa catctgattg tttttttgct cgtgaaccta
     8461 cgacagaggc tccttcaata ccaacaaata cccaaagcat caccatgatg caactcaata
     8521 cttgactgga aaacgggatc ggctgtccat tcgcattcaa tataatgtcg ctagaaaagt
     8581 tatcaatgaa ttgttggatc gaaaacgtgc tgatagctac tacaataaat aaaaataatg
     8641 gcactaattt acagaatgtg acgatagcat tgacgaaact agcattttcg actccccgat
     8701 tcactagcca tgcatatccc cataaaaaga ctgtactcat cacgatcgat gccagatttt
     8761 gcccaccttc aaatactggg aagaaatatc ctaatgcgct catcaacaat gttgcaaaag
     8821 ctacgttgcc taaccagcat gtgagccagt aaccccaacc gctgataaaa ccagcgaaac
     8881 ggccaaaacc ttcttctgcg taactaaaga tcccgccttt taaatctgga cgttttttat
     8941 tgatattatt gatcgacaag gagagcatca acattgctgt tcctacgagt acccaactga
     9001 aaatagctgg gccgccagcc gatccggaga tgtccgtcat aataccaaat acgccacctc
     9061 caatcgctga accaattact aaggcagcaa gtggaattaa accaattcct tttttctgtt
     9121 gctctgtatc catgtaagca tcctccaaaa taaaaccgct tttatcaaaa agtataacaa
     9181 acctttttta tttttactta attaataaaa actttaatag aaatataatt ctaccggctt
     9241 ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac caaaaaactt
     9301 aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca ttttatatat
     9361 atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg ctgaaaaaat
     9421 gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg aaaatagtga
     9481 ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag acggcggaaa
     9541 tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat atctggaaca
     9601 tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa acttcaataa
     9661 tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt tcttggcaga
     9721 ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt tagcagaaaa
     9781 caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct ttcgtttcta
     9841 ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt tgctttacct
     9901 gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac gatcatggat
     9961 gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag cgtatcaggt
    10021 gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa gtcaactgtg
    10081 ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc aatagcgtgt
    10141 ttcgttggga agacagagct gactacccca gtaactggag aatagatatg tccgttttct
    10201 ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc acttgcttta
    10261 tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact ttcagctgaa
    10321 gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt cgctaattct
    10381 tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa gccaccagca
    10441 taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat caatgggatc
    10501 aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat tccgatcact
    10561 gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag aggtaatgtt
    10621 actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc tcctttgatc
    10681 atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc tgcacccact
    10741 tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt ttcatcaatc
    10801 atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat cggcaagaat
    10861 aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca gttgatccca
    10921 ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat ttcaatcaga
    10981 ccgatggcaa tcaatgaaag agttggtgta acgatgatat ctaccgcatc aggtactact
    11041 ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac acctaggatc
    11101 ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg tgcttccgga
    11161 ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg tgttgcacca
    11221 aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat cccgtttttc
    11281 aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt tccggctgtg
    11341 actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac aaatgcagga
    11401 atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt agatggtttt
    11461 tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg gtcttttcca
    11521 gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc taatttcacg
    11581 cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc gatgatgact
    11641 tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa ttcagagaca
    11701 tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg gacaatgctt
    11761 tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc ttccaaagtc
    11821 agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa atccgtttgc
    11881 ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca tgacgatagc
    11941 cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt aatcagcttc
    12001 tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg ttggtttgac
    12061 atcgaccatc aagttattga atactttacc tataccaatc atactgatag tagaaatcat
    12121 attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg tcaaaaattc
    12181 tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg cttgtttatt
    12241 gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc caccgatcac
    12301 atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg tcaaattcag
    12361 cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga cagctacagt
    12421 cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta caccgaatgt
    12481 cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg ctcccatata
    12541 aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg gttcgatact
    12601 tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca ttagttgaac
    12661 ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc gttccgttgt
    12721 taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat aaattgatag
    12781 ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac tttagctact
    12841 acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc acccatatag
    12901 cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc tgtatttctt
    12961 gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac atctcttgca
    13021 tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc atctacaggt
    13081 tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc ctgaacgttt
    13141 tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa tagatctaga
    13201 gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc ttgatatagt
    13261 gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc ttgaattttg
    13321 agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg acgtggataa
    13381 taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa ttcatcgata
    13441 tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga ccagtcggca
    13501 atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat tgctttcaaa
    13561 ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga aatatcgacc
    13621 atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg cttttgatat
    13681 gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa gccattggat
    13741 gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat aaatattgaa
    13801 aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga ctagagtata
    13861 tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca tttctaaagg
    13921 ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct aagcaaaagc
    13981 agtgccttga aatattttat ttaaataaca caaaaacata attactcata agaaaccaat
    14041 tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg aaaataagca
    14101 ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc ttcgtcttcg
    14161 atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga ctccccattt
    14221 gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt gcttggcagt
    14281 ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag cggcaagctg
    14341 agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga atggatgatt
    14401 ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc ctgcatctgt
    14461 cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc gttccaagtt
    14521 gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc aaagttcccc
    14581 ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag agaaataatc
    14641 tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat ggcaggtttc
    14701 acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata attcaatggt
    14761 tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc cacttttttc
    14821 cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc catcccaagc
    14881 tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc aaattccgcc
    14941 aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag atgttttggc
    15001 tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg gataatggat
    15061 gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc gattgtaaaa
    15121 tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac gcttaaaagt
    15181 cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc gatatctttt
    15241 aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc tttggatcgt
    15301 ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct agattcagta
    15361 tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga ccattcggct
    15421 ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc attttgagcc
    15481 atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt cccttgataa
    15541 agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc gataaatccc
    15601 cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc cgctacttgt
    15661 tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa atgagtgtca
    15721 tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag tgtgctgaat
    15781 tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc gatttcccat
    15841 atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt atcttttgtc
    15901 tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata aggtagtttg
    15961 ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat aagtcctagt
    16021 ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc ttggctgtcg
    16081 tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct cgtaaaaaaa
    16141 gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac acacaaaaag
    16201 acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt ccttgtctcc
    16261 acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat gcacggatcc
    16321 ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt gtcaactact
    16381 tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt ttttctaatg
    16441 tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata cttaagacaa
    16501 ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa atagcgatta
    16561 ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt ccgtggatcg
    16621 tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg atcttttttt
    16681 ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct cccaacaaaa
    16741 aagggctcca ccaataccgc ctgctgctcc acttcctgga atatcatcta aatccacctc
    16801 ccagaaatct tttatttgct gagcaatctt tcctgcttgt ttatctaaga aagctagcgt
    16861 cgtatctatt cctccttttt gtcttccaaa ttgccaagca gctccttgtt gacctgtata
    16921 tggattggta acatctgcag cgataatcaa ttttacttta gaaacaagaa cgtttcttcg
    16981 atcaaaatga agttttttag tagataataa aggatttcct tctgtgtacc cttctaaaga
    17041 agcacccaag gattggagta atcctagacc accatcgcta cacccgcttc ctcctaaagt
    17101 gacaataatt tgttctactt cttgaagcag tgcatcttta ataagttctc ctaaggcata
    17161 actggaagca agtcggatag tttgatccgt tggtttatca agtagatcta accccaatgc
    17221 ttgtgcagat tcgatgactg cggtgttttt tccttgccaa gttgtcaata aatagctgat
    17281 ttttcgctga cggaataaga gatccatccc atcaatggta cgccatgttc ccctgagcgc
    17341 gtaatagatt gcttctgcag tattttctcc gccatctgca atcggcacat tgatttttct
    17401 tcctttacga tcttcccagc catttagaac tgcctgattt agttcaaatg aagaagcact
    17461 tcctttaaat gaatcaattg ccgccaaaat atccatctgt atccctcact ttcttcttaa
    17521 gtaagacact ttttttcatt tttttcaaac aaaacaatgc tattttcttc catttttcta
    17581 tatactaaaa gggaactcat tgaataaagg agagaaaaaa catgaaaatc gcaatagctg
    17641 gggccggagc aatgggcagc cgaatcggat tgatgctaca tcaaagcgga aatgaggtcc
    17701 tcttgatcga tcgttggcct gctcacattg aagccattcg tacaaatggg ttgattgctg
    17761 actttaacgg taaagaagtg gtcgcaaaac tgccaatcta ttcaccagaa gaaatcatcg
    17821 aaagcaacga acacgtggac ttgatcgtag cattaacaaa agccaatcaa ttagatgata
    17881 tgttttgttc gatccaatcc attattactg acaatactta tgttttgtgt ttactaaatg
    17941 gattaggtca tgaagatgta ctggaaaaat atgtgccaaa gaaaaatatc ttatttggga
    18001 tcacgatgtg gacagctggt ctagctggtc ccggaaaagt cacattgtta ggtgatggag
    18061 aaatcgaact agagaacttg gaaccagaag gcgaagcatt tacaaaaaaa gttgtagaag
    18121 tctttcaaga agcaaattta aatccgatct atagccataa cgttcgttat tccatttggc
    18181 gaaaagcttg tgtaaatgga acattgaatg gtttatgcac gattttggat tgtaatattg
    18241 ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg aacaatcgta tctgaatttg
    18301 cttctatcgc agcaaaagaa ggaatcatac tagatcaaga agaagtgtac caacatattg
    18361 cttctactta tgatcctgac aacatcgggt tgcattaccc atctatgtac caagacctga
    18421 tcaaaaacca tcgtttgact gaaatcgact acatcaatgg tgccatttgg cgaaaaggac
    18481 aaaaatatga tattgctaca ccatattgtg cctttttgac ccaattagtc catgcaaaag
    18541 aaggaatcct tggcgcagaa taaaaggctc tctgatgaga atcagctgaa tcagcagctg
    18601 gttctctttt tttaagccca ataaaagatc aaaaagaaaa cacaactctc atgatgccat
    18661 aagaattgtg cttgtttgtt tcagtacata atctcttcaa aatgtaggtt ttttatttgt
    18721 tttcaacaga ggagaggtca tattcgtatt gctcataaat agtggaatcg ttagtgataa
    18781 atcgttttgc ccaattttct gcatcataaa attccaatag ctctgatagg attggcttaa
    18841 atggtgtatg cagaaattcc ggtaattcat ctaaatcacg ttctcgaagc aaaaaaatat
    18901 tttcaggacg ataaaaatga taatgagcaa tcgcttgatt atccgtcact agtttctttt
    18961 gaaaatacat ggattcaaat gtccgcaaag tcactccttg ctgaccctcc cgcaacagtt
    19021 ccagaatccc gcgcgattcc cctgtttttt tcaggtactc ttcataagta agatatccag
    19081 gttgttcatt ccctcgatgt ggaagaatga aatagttggt cgtaacatct ctttttataa
    19141 attcctgctt atagctttct gctcgttctt ttctcccatt attcgttgca ccaaaaaata
    19201 gatccgtctt tggttcgcta gaagaaaaat tcatatgttt cgaataaaat gaactattat
    19261 gtttcagccc aagttctttt gcttccaaaa aatcaaaatg ataaatctca tcgattgccg
    19321 gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc tttgaagtga ttccaaaaaa
    19381 ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg agccatctcc atatcaaatc
    19441 cataatctaa aaaaacgatc agctgataat ttgccaactg ttgtctagct gatttaatga
    19501 attccgactg tagtaaatct tttccggacc aaacatctaa agagcggatg gaaggcaaaa
    19561 aataaagtga tttatttttt gcaactagca gtacgtcatt tttgttcatc ttttccgcct
    19621 cactttctct gtcacttttt cctatgatag cctgtttaaa tagtaaaaag ccagtaaaac
    19681 gtttcctata tttgtatgaa aatttaacat ttgtatgaaa atatgatgaa ataaatcatt
    19741 cttctattaa aattcgccta aaagttttct gaatagttgt aattgtctga aaatttgtta
    19801 tcattagtca ttgttaaata ttgctattta ttcttaatat aaattttgat tgtgaattta
    19861 aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc tccagacata
    19921 tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga agtggcgaag
    19981 cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg ctgattttat
    20041 atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg catcacggat
    20101 tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact gattgggtct
    20161 actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct tcctttttgc
    20221 agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct gttttaacta
    20281 cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg ctggcttttg
    20341 ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc ggtcagcaaa
    20401 tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc
    20461 ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt
    20521 ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag
    20581 caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt
    20641 tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat
    20701 ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt
    20761 tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga
    20821 aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac
    20881 gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg
    20941 gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga
    21001 tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag
    21061 gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga
    21121 actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc
    21181 aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt
    21241 atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa
    21301 ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct
    21361 gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa
    21421 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg
    21481 gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt
    21541 cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg
    21601 ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt
    21661 cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct
    21721 tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat
    21781 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag
    21841 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa
    21901 aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg
    21961 ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    22021 aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
    22081 caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga gagacaaatg
    22141 gaaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    22201 acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa gaaaaatttt
    22261 gtgaaatggc tttccacatt tttgtataaa tttggcttaa taccgagaaa ctggcttttg
    22321 aataccgttt attcgtaaaa gggattgtga caaaagtctt gtcacaatcc ctttttatgt
    22381 ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt
    22441 gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa
    22501 aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg
    22561 gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct
    22621 aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga
    22681 ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt
    22741 gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag
    22801 atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca
    22861 gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca
    22921 gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg
    22981 ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc
    23041 ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa
    23101 atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct
    23161 ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt
    23221 gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga
    23281 tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct
    23341 ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca
    23401 atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca
    23461 aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt
    23521 ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag
    23581 attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg
    23641 tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta
    23701 ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca
    23761 agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta
    23821 gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg
    23881 attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt
    23941 accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga
    24001 ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa
    24061 caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag
    24121 atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact
    24181 ttcacacaca agtttatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact
    24241 atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga
    24301 gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta
    24361 gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta
    24421 ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt
    24481 ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat
    24541 aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac
    24601 tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat
    24661 actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct
    24721 ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt
    24781 ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca
    24841 tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg
    24901 actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat
    24961 aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg
    25021 ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca
    25081 atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg
    25141 catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg
    25201 ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct
    25261 acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag
    25321 ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga
    25381 tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc
    25441 tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct
    25501 tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt
    25561 tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt
    25621 gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct
    25681 acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg
    25741 gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg
    25801 ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga
    25861 gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca
    25921 gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc
    25981 gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa
    26041 gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat
    26101 ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca
    26161 tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct
    26221 gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa
    26281 cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg
    26341 attactgata aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt
    26401 tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc
    26461 acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac
    26521 ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact
    26581 ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc
    26641 gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc
    26701 accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat
    26761 gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt
    26821 agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat
    26881 cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa
    26941 ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc
    27001 ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt
    27061 ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta
    27121 aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa
    27181 tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat
    27241 ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat
    27301 gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg
    27361 tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc
    27421 catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga
    27481 tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa
    27541 gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat
    27601 tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat
    27661 aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac
    27721 gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc
    27781 ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca
    27841 attctttggg agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg
    27901 gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga
    27961 cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct
    28021 ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc
    28081 cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt
    28141 tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc
    28201 atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat
    28261 tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct
    28321 gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc
    28381 cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc
    28441 ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg
    28501 gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc
    28561 ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt
    28621 tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa
    28681 agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac
    28741 atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct
    28801 tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct
    28861 cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct
    28921 aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact
    28981 tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct
    29041 gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag
    29101 tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga
    29161 taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct
    29221 ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct
    29281 tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt
    29341 tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc
    29401 ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa
    29461 ccagccttct cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg
    29521 tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa
    29581 gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa
    29641 ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt
    29701 tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc
    29761 ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc
    29821 cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata
    29881 tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca ttccattttt
    29941 ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat
    30001 catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg
    30061 tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat
    30121 atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca
    30181 aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta
    30241 cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa
    30301 atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat
    30361 tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt
    30421 ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga cggcacactg
    30481 aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca tgttctttca
    30541 gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa
    30601 tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct
    30661 tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta
    30721 cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag
    30781 aaatgttcag catttcttca atcgcgcctt ttgtgatcat ttgtgtttta ccagctggat
    30841 cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt
    30901 gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta
    30961 acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat
    31021 catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg
    31081 tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat
    31141 ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa
    31201 cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag
    31261 cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg
    31321 acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa
    31381 ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa
    31441 caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt
    31501 aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc
    31561 tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat
    31621 ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca
    31681 taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt
    31741 tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga
    31801 cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta
    31861 atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg
    31921 tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat
    31981 tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg
    32041 catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt
    32101 gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc
    32161 gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag
    32221 aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat
    32281 ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg
    32341 aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg
    32401 caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa
    32461 gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct
    32521 tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct
    32581 cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg
    32641 gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt
    32701 tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca
    32761 ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa
    32821 cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac
    32881 gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat
    32941 tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt
    33001 acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg
    33061 tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag
    33121 actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg
    33181 taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg
    33241 cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt
    33301 tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat
    33361 tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa
    33421 tctgccatca atgtttcata gatcactgta cttctttctt tatgtgataa gctccgtttc
    33481 atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc
    33541 aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc
    33601 attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga
    33661 aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct
    33721 tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt
    33781 tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta
    33841 cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt
    33901 cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct
    33961 gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc
    34021 actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg
    34081 gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata
    34141 gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa
    34201 atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa taccaacctt caagcattag
    34261 aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca
    34321 acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag
    34381 acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt
    34441 ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag
    34501 agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct
    34561 ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta
    34621 ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa
    34681 gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta
    34741 tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca
    34801 tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc
    34861 tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct
    34921 ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg
    34981 taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt
    35041 gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa
    35101 ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac
    35161 ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc
    35221 ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa
    35281 cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca
    35341 gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct
    35401 gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata
    35461 ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc
    35521 gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc
    35581 atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta
    35641 gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact
    35701 tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt
    35761 ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat
    35821 gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata
    35881 agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata
    35941 ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag
    36001 tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact
    36061 ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata
    36121 atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct
    36181 ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg
    36241 ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag
    36301 taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga
    36361 ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc
    36421 ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta
    36481 ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga
    36541 ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag
    36601 ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct
    36661 tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca
    36721 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt
    36781 ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag
    36841 ctgaccctcg gtattagatt atgtcaggac gctaatttta caacctcttg gtaaacggtg
    36901 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctattt
    36961 tttgaaattg ctccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg
    37021 gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    37081 ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca
    37141 ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt
    37201 ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga
    37261 ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc
    37321 cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt
    37381 tccatttttc aaacgggcta ctttgtcgcc aacatgagtc aaaagcaaaa cggtcaaatg
    37441 cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat
    37501 aaattgattt tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt
    37561 ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga aattatctac
    37621 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    37681 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    37741 aaacggtctt tttttcctca tgctttcctc cactcctatt taattttgac tattctatta
    37801 tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt
    37861 ctttttcaca aaatggtaag aatggagaat ttcacgcatt tgctctcaac tattctttct
    37921 taagcctcaa atccgttttt atctgatacg tctttgaacc aatgacccga ttttttgatt
    37981 gttctttttc catcttgtgc taagtctact gaaataaagc catatcgatt cttatatgca
    38041 ttggtccacg accagttatc catacatgtc cacatatgat agcctaaaca attggcacct
    38101 tcttgaatgg cttgatggac atatttcaga tgagattgga caaattcgat ccgataatca
    38161 tcgtggatca tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt
    38221 tcagaaataa agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag
    38281 attccttgtt catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca
    38341 taattgtcga aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt
    38401 gcttttaccc ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg
    38461 ataaccgtca aatcctctgc atggatcaca ggcatgagat caagttcttt tactatctca
    38521 atcaattcac ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac
    38581 gcatctgcta tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt
    38641 ggtgtcagat tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg
    38701 atggctaacg ctgaagccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct
    38761 gactcttctg gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca
    38821 ttatgagtaa accatttttt tactttcgaa ccaaacaatt caaaacaact tttggcatat
    38881 ttttcatagg cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc
    38941 ggcatatcga aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca
    39001 aaataacgat gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc
    39061 aaacggctcc attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt
    39121 acatcctctt gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag
    39181 aacttttgcg gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcgcct
    39241 gaaatccttc cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga
    39301 aattgatatt ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc
    39361 atgtatgata ttttccatca caatcacttt ttcattttct tcctcttctt ttttgattcg
    39421 ttttaacagc tgttctgctg ctcgttcccc gattttatct gttggtggcg tgatcgtgat
    39481 tttcggaagg atactcagat cgtttttatc taaataatca tagtgactga acgcaatatc
    39541 cttcccgatt ctgacacctt cttgattgaa acattcgatg gcaccaatcg acatattgta
    39601 attgctgaca aaaatagcag ttgccccttt ttctaataat gcttttgccc cgtaatagcc
    39661 gctttttcta gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttggac
    39721 cgcttctttg gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat
    39781 aaatccgatt tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc
    39841 tcgatcattc actaatattg tatcaatacc tgctggtcct tctggacaat tcaatgcgat
    39901 aactggaata ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa
    39961 gacaatcagc ccatcgatca catgcttcat caggtattca attttttgtt caattttttc
    40021 tggctgatcg gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc
    40081 gatccctgag acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat
    40141 agagaagctt cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc
    40201 tgaaatcgct tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta
    40261 acgggaaact gttcccactg aaacaccagc catcttagcc acatcacgga tcgatgccat
    40321 acattctcac tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac
    40381 cgcattcttt gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga
    40441 gcaacttcct ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat
    40501 atagcatttt tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt
    40561 ttttgccttt ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt
    40621 ttctcttcca tccgtttgaa gttttacatt ccaatggtat aatttcttta tcctatcaaa
    40681 tgggggaagt attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac
    40741 attgactggc tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc
    40801 ttctgtctta aaagcggaaa aagcagatac caccaacagt gtatatacgc taaaagtacg
    40861 taaagatatg gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac
    40921 acgcacgtat cagacacaag gaactgattc ttctgaatca tctgaagaaa aaacagtgct
    40981 gacagatatc aagatcacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa
    41041 aaaagaaaca acagtcatat ttacaaaaat cggtaattac cgtttgaagg atgaggatgg
    41101 caacatctac agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc
    41161 tgctatagta aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg
    41221 attattgtac atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct
    41281 tgagcatatg cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt
    41341 atcctgttca aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact
    41401 atggctgatt tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa
    41461 aacaaacagt ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat
    41521 atttgaatat aaaataacca aacgatagaa aggcttattc tacatcacgt agtaattttt
    41581 ctttcccgtc gtttggtttt ttatttttag cctaattctt gaccttttgc atgctggtta
    41641 tccgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga
    41701 cggatcacct tttttgcatc ttctggattt tctacaaaat cgaatttatc cccatccact
    41761 tgtattttag gacatacatc aaaatcttca taccattgat tatagcgggt gttcaacgtt
    41821 tcataatagg tatataacgt ctcatcgctt tctagctgtt catagtcacg cccacgtttt
    41881 ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggccgt
    41941 ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc
    42001 tgtacttcga tatccgttac ccgacctaaa tcagcgttta gatggaacaa caggctatct
    42061 tcatagatgg atcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa
    42121 aatcgcttgt tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg atttgaataa
    42181 aagagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct
    42241 tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc
    42301 actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat
    42361 gtttcaaaat catattcacg tacagcttgg tctgatttta aatacgtatg gagactttgt
    42421 gtaatcatca agttcgtagg taccgtctct tcttgtacaa tgatcatcgg ttttttcaac
    42481 atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca
    42541 aggatagcaa caacaaattc tgcatttgtc agttcttcca tatctttttc gtatacttcc
    42601 tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag
    42661 aaacttgtga ctgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt
    42721 tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca
    42781 ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt
    42841 tggaaaagat tacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt
    42901 ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttgt
    42961 cgggcgtcag accgattgct atccaagaaa gaagttgtga gaattcctcc aatgtaccta
    43021 gacctccagg gaaaacgagg acagcatctc ccttttcaaa taataatacc tttctttcat
    43081 cgatcgtttc tgtaggaata aatgtagtaa catcttttcc aggcaattca gtttcaaaaa
    43141 gcccattcgg atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca
    43201 ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatgt
    43261 attttcctaa cgtttgcgca ataaatttgt aactttcttt attaccaaaa cgggaaccgc
    43321 aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac
    43381 acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggtat ataggataga
    43441 cgccactata tatagtattt agaaaaaaat catttttcag ccattttata gataaaacaa
    43501 aaataaataa cgtgttttca tcgatatgaa atgacttact tatagaagat actgggattt
    43561 cgtcctatat gccaataacg tgtattattt taaacaacaa atacttgttt cttttattaa
    43621 cgaaaaaatc attttgttct cgtgttatgt ttcactgata aatgatgttt ctctaaataa
    43681 aaaagccgac gaactaaacg atcaaaattt gatcattttt gttcatcagc ttctcctaaa
    43741 aatctttttg ttaacgataa atagctggat ttgtaaaaat atcacttgaa tcgtcactgc
    43801 tggaagaaaa ttcagtgata accgcacctg ttgcgcctgc ctggaactag tgtttgatat
    43861 tgggctcgaa cgtgtactga tctctagcat gaagaataat ttcttttttt actgtataat
    43921 aactttcttt cctttttgga atagtacagg aaagtttttt tgtttccttt ccttcgacat
    43981 aaggtatacc agaccctttc gacaacgaaa ggttctcatt tttccttcat cgtcatttca
    44041 aggagggtgc cgatgctctg gacagtttcg atcgctccta aaccaaaatc tgtatattcg
    44101 attctttcta actctgattc tttcatacca ttcctgtctc aaggtacatc tattgtactt
    44161 ttttcttgta atccattctg cctctcctaa ctaattgtca aatcatccca agatatgtct
    44221 tcttcctctt tttcttcttg gatgatttct gcattcggat ctactggttt tttcaataat
    44281 aaaatggtta ttcctgtgac aaaagaacca gctaacaatg caccaatgaa tgcccatggt
    44341 ctggtcatcg tagcgactgc aaacattccc gatgccggaa tacggctatc tgcgccccaa
    44401 aacatgctta atccgccacc tactgtccca ccgactcccg ttgctatgac attgcgtgcc
    44461 aagtcgttca atacgatagg gaaacatccc tctgtgatct gacagatccc cataggaaaa
    44521 gcggtcttca atgtatcgat ttcttgacga ttgagtatat ttttacgaat gattttccct
    44581 aagaaataag cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat caatacagtg
    44641 attggttcat agattccttc tgaaaatcgt gccacacccc cttatccaga ataaatggta
    44701 atccagaaac agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc
    44761 tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca acctcttttt
    44821 ttatttatgt ttcataatca atatggtaag gatgcagctc cgtatggtag accgcctccg
    44881 aatattcaat catttcccca atttgcgtag acgatttccg tatccgtttg atgccgatct
    44941 gtgcgtctgt tttcagaatt ccttgttctt cttctgttaa aaatactgcc gagaaatcat
    45001 cagaaaatga gtcaatagta tatccttttt tcgtcagcaa acgatacaaa gattctttgc
    45061 caaattcttc tctgctgaat tgcatcattt cttgtggaag ataatgaaca aaataaatat
    45121 aaggctgatt gttcaaataa tataagcgct taaaacaaac agcttgctca ccgaaaaagg
    45181 aaaacaaagg atcttccttc tttatatgga ccaattgcaa atccagcgtt tctttccgga
    45241 cttcatatcg ggagttctcc aagatttgcg taaagcttgc cgctttggat aatcgattgt
    45301 aaggacgatt gcttagaacc gtcgtcccac gaccactttt ctttgcgaca tattcatctg
    45361 atgccagcag ttcgattgcc tttcggacag tgattttgct gacttgaaac agttcttcta
    45421 attcactttc tgtcggcaac atcgaaccga ctggatattt tcctgagaaa atatcttctt
    45481 taattttatc tgcgacttcc aagtatagta cacgtgtcct tttcataacg tcaccctttg
    45541 ctttactatt actaaaagaa tagcacataa aaaataaaaa aagaacacaa atcacatgtg
    45601 agaagtgtgt tctttttcat tcagctgctt taattacaag tttacgccat ttgaggcaat
    45661 tacttcttta taccaatcaa aagatttttt ctttgatcga ttcagtgtcc catttccttc
    45721 atcgtctaga tccacataga taaagccgta acgtttactc atttctcctg ttgatgcact
    45781 gacaagatcg atacatcccc aaggcgtata gcccatcaaa tctacaccgt ctgctactgc
    45841 attagccatc gcttcgatat gacgacgtaa ataatcgatc cgataatcat caataatcgt
    45901 gccatcctct tccatatgat caatggctcc aagaccgttt tcaacaatga ataatggttt
    45961 ctggtaacga ttgtataatt cattcaatgc gattctcagc ccttctggat cgatctgcca
    46021 gccccattca cttgcctcaa gaaacggatt ttttactcct cccagcaagt tcccttctga
    46081 agttgctgct tgtggatcag tctcattgat tgccgtagac atataataac taaatccgat
    46141 gtagtccact ggatattctt ttagcaactg gaagtctttt tccgtttgct ctaatttttc
    46201 cggatcgact ccatattttt tatgggtacg cgctgtgtat gcagggtatt ctccacgtac
    46261 ttggacatcc gtgcagaaaa aattgaattc ttggttttgg atcattgttg ctgcctgatt
    46321 gactggattg gcatcgatgc tgtaagttgt tgcatagata atcatgcagc cgatttggat
    46381 atccgaacgc aattcgtggc cgatttttac ggctttgcta cttgcaacga attgattatg
    46441 ccatgcttgg aaaatatttt ggtactctcc tgctccattg ctttttacta atccttgact
    46501 taaagctgga aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat atttgacttt
    46561 actgctgtat ctctctaata ctgtctgagc aaatcgttca tagaagtcga tcagttttcg
    46621 atttttccag ccaccgtatt cttttgccaa atgaagaggc atttcgtaat gagaaatcgt
    46681 gatcacaggt tcaatatcat attttaagca ctcatcaatc agctgatcat aaaattctaa
    46741 tcccgcttcg ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc aggcaatcga
    46801 aaaacgataa catttgaatc ccatttccgc aaacaaggca agatcttctt tgaaacgatc
    46861 gtaatgatcg attcctcgat gattcgggta aatatatttt tcttgatcaa tcgtccagtc
    46921 aaattcttca ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag catctgcaac
    46981 ggatagtcct tttccgtctc gatcataagc cccttctaat tgatttggca gcagttgctc
    47041 caccccataa aaatttttct gaaaaacgtg attgcatcct gatttcctcc ttattttaca
    47101 attgccagaa catcttcgtt tgcaataatc tctttttggt ctaattcaag tatatcggca
    47161 aatgcatcag tattcgtaat gacaactgga gtgattacgc tatagccagc tgctctaatt
    47221 gcttcgatgt caaaacgaat caataaatct cctgctttga cgagatcccc ttgtttgact
    47281 aagatctcaa agccattccc atttaattct actgtatcca tcccgatatg aatcaaaatc
    47341 tccgctccgt cagtcgttgt gatccctaca gcatgtccag ttggaaaaat agtcgtgatc
    47401 tctccgtctg caggtgcaac tagttctcct acttctggat caatagcgat accttttccc
    47461 atcacgccag aagaaaaaac ttggtctggt acttcactca atggcagtac tttacccgtt
    47521 aaaggacttt ttaaggtatg ccgtgctgaa gtaatcggtt ctcctgcatt cgcatgtttg
    47581 ttttctaatt gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt acctacgaat
    47641 gcaagaacaa aagcaattcc tgtagctatt actgctactg tcacatttga ctctacacca
    47701 tccaccgtat taatgaaagt cggaatcgtc aataagctga ccaaactgct ggagaaagct
    47761 tttactccgg aaaatccgat aatagctcca ccaatacctc cagaaataca agcggcaatg
    47821 aatggtttct tcaaaggtaa tgtcacacca tacacagttg gttcagtgat tccaaataac
    47881 gaagtcactg tagaagaaat ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct
    47941 acagctaaag cagccccacc ttgtgccaag atggctggaa gaagcattgg catcaataca
    48001 tcaaacccat attgttcgat attcaaaaac atgatcggta cgaatcccca atgcatgccg
    48061 aacatcacga atacttgcca taaacttccc atgattagac ctgctacgat tggactaaat
    48121 ccataaatcg agttgaagag accgcctaaa gcattaccgg caaccgtccc gattggtcct
    48181 aaagcaagaa atgttaatgg aaccatcact actaaaacga caagcggaac taaaatcatt
    48241 tgcagaaatt gcggaatcac tttttttacg aatggctcta atttactttg aacccatacg
    48301 gctagaatga ttggtatgac agaagaagta taaccagatg gtgaaagaat cactgggatc
    48361 ccaaagaaac tgatagcttc tcccgcacca gcaactgctg tgatagctgg atacacaagt
    48421 gccatagcaa ttgctactgc taaaaactgg ttggctttga actttttagc tgctgtaaaa
    48481 gcaagcatga caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa tatttgataa
    48541 gtaccgctat cagctgtcag ccagcccatc accgttgcca atgataagaa tcctttcaat
    48601 accccggcgg ctgccatcgc ccctagaaaa ggtgtaaata tactagagat aatatcgatc
    48661 agacgattca aaatattttt ggactcttct tttccatcct ccgaaactgc aagaccttcc
    48721 tcatccacga tcgattgata aacatctgct acattactgc caatcactac ttgatactga
    48781 ccgccacttt gtacgacttg gatcacgtcc gggtcttctt ttaacttttg cgtatctgct
    48841 aaagattcat cttttaatct gaatcgcaac cgcgtagcac aatgtactaa acttttgaca
    48901 ttcttttgtc ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt atccattgtt
    48961 ttttccttct ttctttttta tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa
    49021 acattcgtga acctattttt tcaatccagg ttttgcctgc catacagtaa caatccttat
    49081 gattggatgc tttttttctc acttactact ctttgaatat gaatcgttaa ataaatctgc
    49141 tctgcgatcc ccatttcctg tttttgcgtt tgtcgaagat actcattgat ccgttcgaca
    49201 caatgaaacg cttttggata ctttccttga actaatgcgt agagaaattc atcttgctcg
    49261 tcagatcctt cccctttgag ataacgctgg gcaaaaaact gcagatgagt aatgatacgc
    49321 tgataattca aagattcttc atcgaacacg atgccgaaga atttactgat gatactcagt
    49381 atatctctta ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg
    49441 ctattcacaa aatgaaaggc aataaaaccg gcttcatcct cagaaagttc cacacccgtc
    49501 ttttgtttca cgaacaaaag agcctctttc gctgcttgat attctttcgg ataaaatttt
    49561 tttgtttcaa ataataatgg attaggcagt tggatatttt cttttgctcg tgtgatcgca
    49621 taatgaagat gatcggtcaa tgtcagataa atattaggcg aaaattcaac ttttagttta
    49681 tcttcagcca gttcgactac ttgtttgacc cattcgatct cctctgccgg aatatcagcc
    49741 atcagctgct ggaattggtt tttagcaaaa gaatctctta aaacaaattc tttttggacc
    49801 gcttctggcg aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt tcccatacaa
    49861 acgacttctt ccccgaatcc atttttcgaa atgacgacat tgttattcaa aattttatca
    49921 attttcattt tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc gcctactgtt
    49981 tcttagctga attctggttt tgcctgccgt acagtaacaa tccatttctt attcaaaatc
    50041 atcataccat cgaattttta tttatgcaag ccttttcata taaaaaataa aaaaactgga
    50101 acacaagtct atctagaaaa
//
LOCUS       DAZUMO010000013        49305 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000013 DAZUMO010000000
VERSION     DAZUMO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.013"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..571
                     /locus_tag="ACG8ER_000741"
     CDS             101..571
                     /locus_tag="ACG8ER_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS5049341.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="ACG8ER_000742"
     CDS             793..1896
                     /locus_tag="ACG8ER_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS5049342.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2117..3076
                     /locus_tag="ACG8ER_000743"
     CDS             2117..3076
                     /locus_tag="ACG8ER_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5049343.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3246..3926
                     /locus_tag="ACG8ER_000744"
     CDS             3246..3926
                     /locus_tag="ACG8ER_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5049344.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4016..5050
                     /locus_tag="ACG8ER_000745"
     CDS             4016..5050
                     /locus_tag="ACG8ER_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5049345.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5064..5399
                     /locus_tag="ACG8ER_000746"
     CDS             5064..5399
                     /locus_tag="ACG8ER_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049346.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            5383..7863
                     /locus_tag="ACG8ER_000747"
     CDS             5383..7863
                     /locus_tag="ACG8ER_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049347.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7919..9478
                     /locus_tag="ACG8ER_000748"
     CDS             7919..9478
                     /locus_tag="ACG8ER_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049348.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            9475..10839
                     /locus_tag="ACG8ER_000749"
     CDS             9475..10839
                     /locus_tag="ACG8ER_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5049349.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11010..12080
                     /locus_tag="ACG8ER_000750"
     CDS             11010..12080
                     /locus_tag="ACG8ER_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049350.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12095..12763
                     /locus_tag="ACG8ER_000751"
     CDS             12095..12763
                     /locus_tag="ACG8ER_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049351.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12783..13361
                     /locus_tag="ACG8ER_000752"
     CDS             12783..13361
                     /locus_tag="ACG8ER_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5049352.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13762..14673
                     /locus_tag="ACG8ER_000753"
     CDS             13762..14673
                     /locus_tag="ACG8ER_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5049353.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15051..15968)
                     /gene="rnhC"
                     /locus_tag="ACG8ER_000754"
     CDS             complement(15051..15968)
                     /gene="rnhC"
                     /locus_tag="ACG8ER_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS5049354.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16170..16607
                     /gene="zapA"
                     /locus_tag="ACG8ER_000755"
     CDS             16170..16607
                     /gene="zapA"
                     /locus_tag="ACG8ER_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS5049355.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            16665..17213
                     /locus_tag="ACG8ER_000756"
     CDS             16665..17213
                     /locus_tag="ACG8ER_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS5049356.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17328..19688
                     /locus_tag="ACG8ER_000757"
     CDS             17328..19688
                     /locus_tag="ACG8ER_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5049357.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19775..20089
                     /gene="trxA"
                     /locus_tag="ACG8ER_000758"
     CDS             19775..20089
                     /gene="trxA"
                     /locus_tag="ACG8ER_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS5049358.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20271..22049
                     /gene="uvrC"
                     /locus_tag="ACG8ER_000759"
     CDS             20271..22049
                     /gene="uvrC"
                     /locus_tag="ACG8ER_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS5049359.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22194..23093
                     /locus_tag="ACG8ER_000760"
     CDS             22194..23093
                     /locus_tag="ACG8ER_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049360.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23086..24348
                     /locus_tag="ACG8ER_000761"
     CDS             23086..24348
                     /locus_tag="ACG8ER_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049361.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            24509..26407
                     /locus_tag="ACG8ER_000762"
     CDS             24509..26407
                     /locus_tag="ACG8ER_000762"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS5049362.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(26737..27456)
                     /locus_tag="ACG8ER_000763"
     CDS             complement(26737..27456)
                     /locus_tag="ACG8ER_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS5049363.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    27646..27693
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            27793..28125
                     /locus_tag="ACG8ER_000764"
     CDS             27793..28125
                     /locus_tag="ACG8ER_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049364.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28112..30115
                     /locus_tag="ACG8ER_000765"
     CDS             28112..30115
                     /locus_tag="ACG8ER_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS5049365.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30134..30604
                     /locus_tag="ACG8ER_000766"
     CDS             30134..30604
                     /locus_tag="ACG8ER_000766"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS5049366.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            30625..31209
                     /locus_tag="ACG8ER_000767"
     CDS             30625..31209
                     /locus_tag="ACG8ER_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFS5049367.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31224..32210
                     /locus_tag="ACG8ER_000768"
     CDS             31224..32210
                     /locus_tag="ACG8ER_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS5049368.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32200..32511
                     /locus_tag="ACG8ER_000769"
     CDS             32200..32511
                     /locus_tag="ACG8ER_000769"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS5049369.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            32533..34314
                     /locus_tag="ACG8ER_000770"
     CDS             32533..34314
                     /locus_tag="ACG8ER_000770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS5049370.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34307..35683
                     /locus_tag="ACG8ER_000771"
     CDS             34307..35683
                     /locus_tag="ACG8ER_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS5049371.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            35690..36325
                     /locus_tag="ACG8ER_000772"
     CDS             35690..36325
                     /locus_tag="ACG8ER_000772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS5049372.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36355..37710
                     /locus_tag="ACG8ER_000773"
     CDS             36355..37710
                     /locus_tag="ACG8ER_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5049373.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37902..39296)
                     /locus_tag="ACG8ER_000774"
     CDS             complement(37902..39296)
                     /locus_tag="ACG8ER_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5049374.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            39388..40359
                     /locus_tag="ACG8ER_000775"
     CDS             39388..40359
                     /locus_tag="ACG8ER_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5049375.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40356..41138
                     /locus_tag="ACG8ER_000776"
     CDS             40356..41138
                     /locus_tag="ACG8ER_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS5049376.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41307..41678)
                     /locus_tag="ACG8ER_000777"
     CDS             complement(41307..41678)
                     /locus_tag="ACG8ER_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5049377.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            41812..42255
                     /locus_tag="ACG8ER_000778"
     CDS             41812..42255
                     /locus_tag="ACG8ER_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5049378.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42298..43107)
                     /gene="yidA"
                     /locus_tag="ACG8ER_000779"
     CDS             complement(42298..43107)
                     /gene="yidA"
                     /locus_tag="ACG8ER_000779"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS5049379.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43126..44493)
                     /locus_tag="ACG8ER_000780"
     CDS             complement(43126..44493)
                     /locus_tag="ACG8ER_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5049380.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(44477..45307)
                     /locus_tag="ACG8ER_000781"
     CDS             complement(44477..45307)
                     /locus_tag="ACG8ER_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS5049381.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            45470..45907
                     /locus_tag="ACG8ER_000782"
     CDS             45470..45907
                     /locus_tag="ACG8ER_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS5049382.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            45983..46603
                     /gene="rpoE"
                     /locus_tag="ACG8ER_000783"
     CDS             45983..46603
                     /gene="rpoE"
                     /locus_tag="ACG8ER_000783"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS5049383.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            46792..47298
                     /locus_tag="ACG8ER_000784"
     CDS             46792..47298
                     /locus_tag="ACG8ER_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5049384.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            47315..48214
                     /locus_tag="ACG8ER_000785"
     CDS             47315..48214
                     /locus_tag="ACG8ER_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS5049385.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            48301..49185
                     /locus_tag="ACG8ER_000786"
     CDS             48301..49185
                     /locus_tag="ACG8ER_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5049386.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        16309 a         8497 c        10425 g        14074 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac ttcgccgatt gtaaaattaa
     2041 gctagacaac taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta
     2101 taaggagtga gtacaaatga cctataacca tcttacacca actgaactcg taatgataga
     2161 agcttatttc aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg
     2221 acaaactatt tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta
     2281 taaacaatat aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga
     2341 gcaacaggaa tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg
     2401 tcgagcagag tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa
     2461 aacctttgac actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga
     2521 aaaaagagga aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca
     2581 gtttgatcat gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag
     2641 cgcggtcatc acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg
     2701 cagacaggct aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa
     2761 cttatttaaa tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag
     2821 taataccaac gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt
     2881 gaatgaacat tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa
     2941 tggtgtatct gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa
     3001 atcattacac tatcggacac caatggaagt atttttgagt tacgtcacaa aagatgattt
     3061 gtctaactta atttgacaaa cgaaacttaa taaaaaagga agaatattca tgtaaacgat
     3121 tttattgttt ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt
     3181 catgaggatt ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat
     3241 ttattatgaa aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct
     3301 tggcaacacc agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg
     3361 aaccaacaaa taccgtggag cctcttaacc ctactgatcc ttcaaaacca gtagagccag
     3421 cagatccgga aaatccagca actggccaga caggtccttt aacactggat gtagttcccg
     3481 aattgccatt tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt
     3541 caaaaaacga tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc
     3601 aagcacaagg gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt
     3661 tgcaaggcgc tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat
     3721 ccactggacc aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa
     3781 tatttacagc agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa
     3841 atatcacact gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga
     3901 acctcgtggc tggcccagta gcatagagta tgaaataaat tcaacaaact gcggtgaaaa
     3961 tactataggt gaaggatttt ttaccgcagt tgttgttgaa aggatgtggt ggacaatgaa
     4021 tcaaagtaaa cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc
     4081 gtcaatcagc caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga
     4141 gaaccaaata gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca
     4201 gacactagaa gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt
     4261 caatccggcg gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt
     4321 agatgagaca ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa
     4381 tgtatcagca catgcagaaa caactgtacc tatagaagtg aaaatcccag caaaaagctt
     4441 taagggacgt gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa
     4501 tgctacagaa ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca
     4561 agaaagtcaa gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt
     4621 caacgcaaag ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa
     4681 tttgatattt acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa
     4741 tgcattttta gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa
     4801 agcaaaagcc ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg
     4861 gcgatttaca caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc
     4921 cgtttttgca gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt
     4981 tctagtgtta tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga
     5041 aacaacatga tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa
     5101 gtttgctgtt cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga
     5161 ttacatttta tgatctaagt ccaattccgc atccaagtgg ttcagtcaat ggcgcatcaa
     5221 aacagcagac acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga
     5281 cgaatgaaca aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg
     5341 cgtggttgtt gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa
     5401 ttttcatttt ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt
     5461 tcaccttcgg cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg
     5521 tatgaaaata tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc
     5581 caactaactg gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca
     5641 aatgatcctt ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca
     5701 aatgtacttg atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat
     5761 ttgccggaac atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac
     5821 ttgtatattg ccggtgcccc catcaaggtt actagcggaa gtttattatc agcaggaagc
     5881 cataccttgc gttttgggat ggggtcgtgg gatacaaata ctctattgac agatattatt
     5941 gctattatta cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca
     6001 gctaatgtcg cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt
     6061 ttgaccaagg gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt
     6121 tatgattcga ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca
     6181 ggtagatttt atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct
     6241 gcaagtgcag agataaatac gtggaatagt tacatctcac caagtgattc aacaggaaaa
     6301 tataatacat ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc
     6361 tctaccaata gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca
     6421 tttagtcgct ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca
     6481 atactgactc atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac
     6541 agtgggaaag atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag
     6601 tacaagctag acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag
     6661 ctcctttctt ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag
     6721 ggttcgatcc attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca
     6781 gatcaacttt tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca
     6841 ctacctgcat tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt
     6901 actaacatgt atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact
     6961 cttcctcaaa taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac
     7021 cttagcagac aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa
     7081 gaaaatgtga cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg
     7141 atctcacttc ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact
     7201 atcgcagata ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata
     7261 ggagatacca ttcatcttac ttacaactat caattaaata ctgtagatac ttctttacaa
     7321 tccgtcagca tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt
     7381 gctaagcttc ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa
     7441 gaattaacat tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg
     7501 ttgaagacaa gttattatga atcaaaaggc gatttttctt ttgatgtgcg ggatactcgg
     7561 cttccaagca cgtctccttg gcaactgaca ggtacattga cttctttatt taagaatgat
     7621 caaggtcaag aattaagcgg aacaaaatta tacttcaatc attctggatc aaagcaattg
     7681 attcagcaag gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt
     7741 ctcgttgact ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct
     7801 caacctggcg caacgtatca aggcatggtc acatgggaac tgacagctgg gccaaccagc
     7861 taagtgatta gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat
     7921 gagaaggaaa cttctgctgt taaaatcaga aatgcttgaa ggagtggact tggaactttt
     7981 cagtttgctt gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga
     8041 aaaaggagag gtttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt
     8101 gaaagaagat ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt
     8161 tatccaagaa gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta
     8221 ttatcattgt tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa
     8281 tggctcattt gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc
     8341 tcgcagagtg aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg
     8401 gcggctgacc ggtccagaaa gtcaaattcg ttatttttat ttccaattat tttggtttgg
     8461 acgtccctat cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga
     8521 tctgctgcaa aaagagttta gttttgctat tacagaagaa ggacgattga aatttgaaat
     8581 atggctttcc attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag
     8641 caatcttcct gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt
     8701 gacccgttac ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta
     8761 tcttttcatg atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt
     8821 gataagcgaa aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc
     8881 gatagaagaa tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt
     8941 gacagtgatg caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg
     9001 ccaagaagga ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg
     9061 tgaaagattg gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa
     9121 tgcccgcttt gaattatttg ctcgctatgc atctatttta cggacgctga tcaagcagtt
     9181 agaattacgt ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt
     9241 gaaagaaatc atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga
     9301 gaaagaaaag cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa
     9361 gtccgctaaa gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa
     9421 ccaattttta aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa
     9481 atcgtgatca ttggtgcttc ccatgaaggc atcacagctg ccctaaatct aagaaaactt
     9541 caaccgaaag cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt
     9601 tcaaatggaa tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat
     9661 aacatgcgga ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa
     9721 cttgatccag acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata
     9781 acctatgata aattaatcgt agcgacaggt tcctctcctg tcaccttgta taaacaaatt
     9841 gaagctgaaa atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct
     9901 ttaaaagaag caaaagaagt agtgatcttc ggtgctggtt acattggatt ggaattagca
     9961 gatgctttga gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta
    10021 tcaagatatt tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa
    10081 ataaattttt atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta
    10141 tccgtacaac ttctgtctca aacaataaag gcagatatgg tcattttttc tgcccaaact
    10201 cgtcctaata cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata
    10261 gtcaatgaat atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca
    10321 gtcagttttg acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat
    10381 atggcaagag cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa
    10441 aagataacag cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat
    10501 gaagcgccat tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca
    10561 caatacgacg aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta
    10621 gaaataattg gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt
    10681 gcggatatcg tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt
    10741 ctgtctgaat acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct
    10801 aagagagcaa atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt
    10861 gtgtggggat ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc
    10921 ggaagaattt tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat
    10981 aaaatgatta tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt
    11041 caaagttacg ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt
    11101 ttttgactgg gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg
    11161 aggcagattc gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta
    11221 ctaagaagat agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc caaacacctg
    11281 gagtcgtgac tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta
    11341 tcgataaaaa tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg
    11401 atgaggcagt aggcgatatt agtttgaaag aagaatatgg tctagccata ggagatacag
    11461 ttaagttatc tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat
    11521 tcaatgtctc accagttctc tatacaacca tcaatgctta ccaagaaatc cgcttcgaaa
    11581 aagaagatac aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta
    11641 atttacctga agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat
    11701 ataatgcgca agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta
    11761 ttgtgatcgg gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa
    11821 tgaaagcaca agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca
    11881 ttttatcatt tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc
    11941 tacctgatgc ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga
    12001 tgttagtagt agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc
    12061 cgttgaaggc aattggataa gaaggaggaa gaacatgaaa gcaatagaat ttaaagaagt
    12121 agaaaaaagt ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag
    12181 tatcgataaa ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt
    12241 tttgactttg gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca
    12301 agaattcagt aatcaaaaag aaagcgaacg cgttaaacta cgttttaaag aaattggctt
    12361 tattttgcaa gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt
    12421 agataagatt aaacatgaac atcgtcaaaa agaagcaaac gaattatttg agcagcttgg
    12481 cgtggataag ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt
    12541 cgcaatcgct cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc
    12601 tagtttggat tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga
    12661 caaaaataaa gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag
    12721 aatatttatg atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa
    12781 aaatggtcca tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact
    12841 tgtttatgtc caagggatat ctcggcactt caacaagaga aatcgcacaa aaagttggga
    12901 tcacccagcc caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt
    12961 tagaaaacca cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa
    13021 cgggatttca agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg
    13081 atttgtttct gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc
    13141 ttttctttct atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc
    13201 aatctgtgct aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt
    13261 tagcacctta catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat
    13321 tgatcgattt atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa
    13381 aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgctt ctttatatag
    13441 gtagtttttc ataaatacaa atttgttatt ctctcatgat tatcagggta tatttttatt
    13501 tattatgtcg gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta
    13561 gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc
    13621 aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat
    13681 cttttacagc cccttttttg cttacttaag ctgattttaa gttttggccc ttagtctgga
    13741 tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt
    13801 atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg
    13861 gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa
    13921 gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact
    13981 accccacttg caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat
    14041 gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc
    14101 aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta
    14161 gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa
    14221 aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca
    14281 acggtttatt tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac
    14341 agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg
    14401 aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac attagcacag
    14461 acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac
    14521 tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat
    14581 aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt
    14641 ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag
    14701 ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt taaacaccgt
    14761 ttaaggaaga ggtcgtgagc ctgccgttca actctgagtc acaaggagca atttcaaaaa
    14821 acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac
    14881 cgtttaagaa aaaggttgtg aaatcaacgt tttgttctga gtattagaga aacaaacgga
    14941 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgaggatcag atgattgaac
    15001 accgtttatt cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt
    15061 agtagcttca aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata
    15121 ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct
    15181 gacggaaggg tgattccagc ttcagctgat gctttgtcta actcttccaa aaagctggca
    15241 cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag
    15301 tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc
    15361 ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc
    15421 aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg
    15481 atctcattgt atttttctgg agataagaca agcagtttat aggggatggt tttttttaag
    15541 atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt
    15601 ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggaccg
    15661 aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt
    15721 ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta
    15781 tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta
    15841 atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa
    15901 taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg
    15961 ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc
    16021 gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt
    16081 atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa
    16141 ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcgg
    16201 tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg
    16261 taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata
    16321 atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag
    16381 aaagaatatt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta
    16441 cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat
    16501 tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt
    16561 taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca
    16621 aaataagaaa aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga
    16681 ttttattttt attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa
    16741 tcatattcgc gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat
    16801 tagcaagtca tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag
    16861 cattttttga tcaggtattt gcctttcacc tagatgaagc tttttatgca ggaactgctt
    16921 ttctgattat tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg
    16981 gattgacgta tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac
    17041 taatcatggc ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag
    17101 atgtggtcca aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc
    17161 catttcttac caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat
    17221 agtgctggaa aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg
    17281 tatgtccggc acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa
    17341 tccttgaaac attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg
    17401 cacaaggaaa agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa
    17461 actggcttca agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta
    17521 tacctaaaat tgaaaatatc cgtccccata tgaaacggat tgagatcgga gcagacttga
    17581 atggagtgga gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt
    17641 tcattgatga tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc
    17701 agttagtcac gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac
    17761 gcgtgactga tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg
    17821 aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa
    17881 gtgatgcgat catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc
    17941 gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc aggtcagact ttatttattg
    18001 aaccaaaaca agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa
    18061 atgagattca acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa
    18121 tcatccacaa tacttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg
    18181 gtaaagaagt aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac
    18241 aagcaagaca tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa
    18301 aagactatca agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga
    18361 agactttagg tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag
    18421 aaagtcaaat cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg
    18481 agcaaagtct atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag
    18541 tcaatgaaaa aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag
    18601 gtgctgctct agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg
    18661 caacaacgca ttatcctgaa ttgaaagttt atggatataa tcgggcaaat acgattaatg
    18721 ccagcatgga gtttgatgtg gatacattaa gtccaacgta tcgtttgctg attggcgttc
    18781 ctggacgaag caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg
    18841 atgaagcgaa gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt
    18901 tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg
    18961 aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg
    19021 aaaaagaaat ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa
    19081 aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg
    19141 tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa
    19201 ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag
    19261 acgaagtgtt ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc
    19321 agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg
    19381 ttgctcctca aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta
    19441 gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg
    19501 tagatcaata tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg
    19561 gaaaaggaac aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg
    19621 tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat
    19681 ttaagtaaag cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata
    19741 ctagtcatag attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac
    19801 atttaatgaa gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg
    19861 tccatgtcgt atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga
    19921 attcagaatt gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat
    19981 catgagcatt ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg
    20041 tgttcattca aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc
    20101 aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt
    20161 taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt
    20221 ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac
    20281 gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata
    20341 aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat
    20401 attttactgg tagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg
    20461 aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga
    20521 aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga
    20581 taacgaatga aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac
    20641 tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc
    20701 gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc
    20761 atttgggaca atgcctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa
    20821 tcgttgatga agtgaaaaac tttttaaacg gaggacacga aaaaatcgag tcagagatcc
    20881 atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag
    20941 accagatccg tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac
    21001 tggatcgaga tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt
    21061 tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag
    21121 cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc
    21181 ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta
    21241 cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga
    21301 atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga
    21361 tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt
    21421 cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg
    21481 atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg
    21541 atgattatgc atcaatgaga gaagtcatct atcgcagata ttcgcgagtg attcgcgaag
    21601 ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa
    21661 aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg gtaaaaaacg
    21721 ataaacataa aacaaatgag ttgctattcg gtacagattt ggctgtagta ccgcttgagc
    21781 gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa
    21841 tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg
    21901 aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata
    21961 tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa
    22021 acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg
    22081 tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg
    22141 cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag
    22201 aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc
    22261 aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga
    22321 cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc
    22381 atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt
    22441 atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa
    22501 gaaaagagat tgaaccaaaa atcgactttt ggatggaaaa atttcaggta aaagggaaaa
    22561 agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca
    22621 cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg
    22681 taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca
    22741 ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc
    22801 gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta
    22861 cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga
    22921 aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg
    22981 ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac
    23041 cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg
    23101 attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata
    23161 ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat
    23221 agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc
    23281 caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa
    23341 gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata
    23401 aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa
    23461 caactgactg gcttgcaatc gatgcttcga gcgagtcaat taggcatttc accagaagaa
    23521 gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa
    23581 ggaaaaatga cagtcggaga agataactcg acagtccaat acgtcgtaag ttatgtagca
    23641 acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct
    23701 tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat
    23761 ttttacggaa aattaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt
    23821 attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta
    23881 gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg
    23941 atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa
    24001 gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatacatg
    24061 cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat
    24121 attccattct tatcttcata tattatgccg attcgcttag caaatgagac agtcggtatg
    24181 ggtggagcat tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg
    24241 tctgcaaaca tgtacaaatc gaatgtactc gtttatagcg aaggcggatt atggagttca
    24301 ttgaaacaat cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa
    24361 atacagaagg aagctggaca tctattgttc ccagcttctt tttgtataga cctattttat
    24421 ttattttact atattggata tatattcatt gaaaatagtg tataacaact ttataatatt
    24481 gagtatacca atttttggag gcgttttaat ggaccgttat ttagatttac tttcagaaaa
    24541 attcccctca acggaggatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct
    24601 tccaaaagga acagagttat ttttgagtga tatacatggc gaatttcctg cttttgatca
    24661 tattctgcgt atcggttcag gaaatcttaa agaaaagatc aaagaccttt tttctgaaca
    24721 aatgacggaa acggatatca gccaattcac tatttttacg gcttatccag aatatgcatt
    24781 aacgactgat tggtacaaag aacaagataa atcgcagctg atccatcatt tgatcgatct
    24841 tttacggttt acgacagtca aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga
    24901 atatagctat atcattgagg aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga
    24961 ctatgtaaaa aaaatcattg aacaacttgt tttgatgaag gaagaagaaa aatttttaac
    25021 gaaacttgca cagacgatcc aaaaatctgt catcgatcat ttgcatatcg ttggagatat
    25081 ttttgaccga ggaactcaag cagccaaagt gatggatcga atcatggatt tttcttctgt
    25141 agatatccaa tggggaaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc
    25201 atgtttactg acacttttac ggattgcagc acgctataat tatttatttg aattagagaa
    25261 agaatatggg ttgaatttac gtccattgtt ttcttttgct catcaaactt atcagaagaa
    25321 tccagtattt gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag aagaattaga
    25381 aaaaatccat caggcacttt ctatccttca attcaaatca gaacatcagt tattggatcg
    25441 tcgacctgaa tttaaaatgg acgatcgtaa attgctggaa aaaatcgact atgaagcatc
    25501 aacgattgat ttagaaggtc agctctatga gttgaaagga acttgttttc aaacgatcca
    25561 gccagatgat ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata gtttgatgta
    25621 ttcattccag cactctttga gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg
    25681 aatgtatctc acgaacaatc agcatgtgct gtttcatggc tgtatacctg tagacgagaa
    25741 gggacagctc ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggctt
    25801 ttttgaaaaa cagataactg aaagcgcaaa agatctgact gcaaaagaag atttagcaac
    25861 cgatttgatt tggtatgcgt ggagtgggcc attttcgcct ttattcggca agagtaaaat
    25921 ggcgactttt gaacgttatt ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc
    25981 ttattatcac ttgcgagatg aagagtggtt cagtgaaaaa attttagcag aattcgacac
    26041 aaaagaagaa gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga
    26101 gtcaccgatc aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat ccaaagctta
    26161 tcaaaaaacg acaggaattg ccggttattc actattatgc aattcttatg gtttccaaat
    26221 cgtgacacat tatccgtttt tgcctattga acaacaattt gcaaaaaaat ccgaccaaac
    26281 aaatgtgaaa aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg atacaacaaa
    26341 aggtatggaa ctgcagcagc agattaccca gctcaaaagg ctgttggact atcgaaagga
    26401 cgattgagaa gaattccagt gaacattgtt ccatagactg gagactgaga caaaagtcgt
    26461 gtcgcagtct ctttttccgg ataaacggtg ttcttctgcc aactcctcgg tcacaagtca
    26521 cactattcaa caaacatgaa aagctgtttt ttgaaattgc tccttgtgac tcagagttga
    26581 acggcaggct cacgacctct tggtataaac ggtgttcaaa agccagtttc tcggtattaa
    26641 gccaaattta tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta atactcgaaa
    26701 ctaaacagct ttttcacaac cccctcagaa agtctattaa aattcattca caaagaaatc
    26761 gaataagcgc tgttcagatg gatgtacacg gtgggtaagt tccggatgcc attgaacacc
    26821 gaggatttta tgcatatcat ctttgctttg aaccgcttca acgagtccat cgttagaaaa
    26881 agcgatggct tttaataaag gtgaaagatc tttgactgcc tgatgatggt aagaattcac
    26941 aaaatatgga tcggggagaa ggttgcttaa caaactgtct gaaacaatct tgacttcgtg
    27001 agtcgtaaat tgcggggcag tgggctgttg ttcatgtttt actgaccact ttggatataa
    27061 tgaaagatct tgataaagtg taccttccaa aacgacattc aatagttgga gtccccggca
    27121 gactgcaaaa attggttttc tttgtttcaa tgcttccaag atcagtgcct gttcaaattg
    27181 gtctcgttga atatttgttt ccgttagctt tggatgagga tcttccatgt aaagttgtgg
    27241 actgacatct tgaccacctg caagaataag tttgtcgatt tgtccaatca gttgaggagc
    27301 tgaatctgga gacatgactg gcagaataag aggcaatcct tgagcatcta aaactgcatc
    27361 cacgaatcct tgcggtgtat agctcacttg atttccttgg aaagtatctg ttgctcgaat
    27421 cagttgattt ccggcaatgc cgatgattgg tttcatttgt attcccccta aagtagactt
    27481 attgtaacaa ggatttaaaa aaaattaaac tatgtaagac ttgcccctat acttatctgt
    27541 tcttgctgtg ttactatctg taaagagaaa tttcgtgtga aattcatgac gatagttgaa
    27601 aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct
    27661 tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac
    27721 taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta
    27781 aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa
    27841 gaaaatgaaa agcgtgctca aacactgcgg cagcagctga agatctatgc aacagaaaaa
    27901 gagcaggaat tggaaacgat ccaaacaaac tatcgacaac aactaaaaca gttggtggaa
    27961 gaaaaagaac aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa
    28021 gcagacaagg tgcaagcaac tttgaaagat caatatcaaa agcatcagag tcaagcgatc
    28081 ggtgcgatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac
    28141 cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa
    28201 cgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtgg agaagtattt
    28261 tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggta atcggttgaa
    28321 agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact
    28381 ccatttgaaa cgtcaagaac tttccttaca agatttggaa gccaattatt ccgaagaaaa
    28441 tttcataaat aaattgaaag aagtccttga tttaaaagaa aagtgggaac aactaactga
    28501 agaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat
    28561 tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc
    28621 aaattttgaa agtttcaagg aagaactgag taagcttgat gacgtttata tggaagaaat
    28681 agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgt
    28741 tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa
    28801 tcctaaagaa aaactgcaag agaccaagca gcaattatcc attgttcacg agcagcaaaa
    28861 gaagataacg acagcgttag gtcagtgcag tggctatatc caagaatttg aatgggtaga
    28921 agaagtcaca cttgccgttg cagaacgaga aaaaataaaa gaacggttta tccatgcgac
    28981 ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt
    29041 gttaaacgaa accgtatcag aagaagatat atacgtcagt ttcgaaacac caaactcaga
    29101 agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga
    29161 attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa caccttggat
    29221 gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt atggtttgct
    29281 gatgctcatt gggacaatac tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg
    29341 tttcgtaaaa ttctttgaga ttctctcgat accatcgatc atctggggat tgatctacag
    29401 ttcatttttc ggacagtcat taccaaaaga actgtttggc atccgcttgc ctttcccaat
    29461 cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat
    29521 acagattttg gttggattat ttgtggcagc aaaggaaaat atcaggagaa aagattactt
    29581 gagtgctgtc agtgatggat ttgcctggca agggatccta attgggatcg ttattgcgtt
    29641 ggttggtgcc atgttgataa aaaatacagc atttgtttac cttggcgctg ggctatcgat
    29701 cattgctgcc ttatgtatct tgatcgttcc gattatccaa acgccgtcta aagtcaaagg
    29761 tgctgccaaa ggtgcgtata atctttacgg actgaccggc tatatcggtg atttagtcag
    29821 ttatacacgt ttgatggcat tgggtatttc aggcggaagt attggagcgg cattcaatat
    29881 gctagttgct ttcatgccgc cagctgctcg attcagtgta gggatccttt tgatcatcgc
    29941 tttacacgca ctgaatatgt ttttgacact gctaagtgcc tatgtccatg gcgcacgatt
    30001 gcagtatgtc gaattttttg ggaagtttta tacaggaggc ggacgtgcct ttgatccgct
    30061 gaaaacggcg gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata aataattgga
    30121 ggatttttat ataatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcggt
    30181 attagcgatg gcaacagcga cgattttttc agggatcggt tcagctaaag gtgttggaat
    30241 gacaggtgag gcagcagcag cattgacaac aagtcaaccc gaaaaattcg ggcaggctct
    30301 gattctgcaa ttgcttccag gcacgcaagg tttgtatgga ttcgttattg cctttttgat
    30361 ttttatcaac ttaggaaatg acatgtcagt tgtacaagga ctgaactttc taggtgcttc
    30421 tctgccgatt gcttttacag gcttgttttc aggtattgcc caaggaaaag tcgcagcggc
    30481 aggtattcaa attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc
    30541 gatggttgaa acatacgcga ttttaggttt cgttatttcc ttcctattag tgttgaatgc
    30601 ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaac
    30661 gaagcagcag agcaggaaag agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa
    30721 aactttgaac aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa
    30781 atcgaattac tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt agaagtacga
    30841 caagaaaatt tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga tgcggtaact
    30901 gaaatggaaa attgggatga atctgaacaa atccagttta tcaaaaatgc attgtattca
    30961 ttacctttga caggaaaagt agcattcatt gctggagaaa aatcagcagc ctatctttct
    31021 cagacgttgt tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa
    31081 actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt
    31141 ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta
    31201 ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa
    31261 gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg
    31321 aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg
    31381 aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg
    31441 aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgttatact ttccataatc
    31501 tgaaggtttt gacaaaagct gagatgacaa atcaaaatct ggaccatttg tatatcgacg
    31561 atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac
    31621 ttcctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca
    31681 ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg
    31741 ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca
    31801 atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg
    31861 tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata
    31921 tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca
    31981 tccgtgaggg aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt
    32041 cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga
    32101 acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt
    32161 tcgaatcaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta
    32221 gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt
    32281 acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg
    32341 atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa
    32401 caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg
    32461 gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag
    32521 gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct
    32581 gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc
    32641 ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct
    32701 ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga
    32761 ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa
    32821 atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa
    32881 aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc
    32941 gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa
    33001 ggaacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt
    33061 gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt
    33121 cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt
    33181 gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca
    33241 gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat
    33301 gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg
    33361 attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca
    33421 aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat
    33481 ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa
    33541 gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct
    33601 tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga
    33661 tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat
    33721 atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat
    33781 tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt
    33841 tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg
    33901 gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg
    33961 gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt
    34021 cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag
    34081 aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg
    34141 tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc
    34201 ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa
    34261 atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata
    34321 tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa
    34381 atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt
    34441 ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt
    34501 acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat
    34561 gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc
    34621 tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga
    34681 tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca
    34741 aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc
    34801 gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg
    34861 aatcactttt gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga
    34921 ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc
    34981 tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt
    35041 tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg
    35101 agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt
    35161 cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac
    35221 aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg
    35281 acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact
    35341 cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca
    35401 tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt
    35461 agccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc
    35521 tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga
    35581 aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat
    35641 caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt
    35701 gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac
    35761 aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt
    35821 gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc
    35881 tgattttgta ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt
    35941 gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc
    36001 tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt
    36061 gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact
    36121 tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac
    36181 acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta
    36241 ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt
    36301 aaaaaatatg ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt
    36361 aaacaaagca aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt
    36421 tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg
    36481 catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga
    36541 ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt
    36601 ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata
    36661 aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa
    36721 gtatcaggtg tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg
    36781 attggtacgg ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc
    36841 tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga
    36901 gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc
    36961 atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag
    37021 aagatgacga ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga
    37081 ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt
    37141 caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca
    37201 gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc
    37261 ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg
    37321 atccagatgc gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc
    37381 cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta
    37441 gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat
    37501 attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca
    37561 gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt
    37621 gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa
    37681 tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt
    37741 agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt
    37801 tcgtttattt tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg
    37861 aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc atattttttt
    37921 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
    37981 gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt
    38041 cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact
    38101 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
    38161 aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca
    38221 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
    38281 ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact
    38341 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
    38401 aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
    38461 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
    38521 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
    38581 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
    38641 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
    38701 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
    38761 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
    38821 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
    38881 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
    38941 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
    39001 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
    39061 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
    39121 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
    39181 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
    39241 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
    39301 tccactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
    39361 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
    39421 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
    39481 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat
    39541 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt
    39601 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
    39661 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
    39721 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
    39781 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
    39841 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
    39901 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
    39961 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
    40021 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
    40081 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
    40141 gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
    40201 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
    40261 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
    40321 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
    40381 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
    40441 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
    40501 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
    40561 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
    40621 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
    40681 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat
    40741 atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
    40801 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
    40861 ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta
    40921 gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta
    40981 atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt
    41041 catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa
    41101 aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa
    41161 taaattggtg gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact
    41221 ttttcaattt aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca
    41281 atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca
    41341 gggtcaatga caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc
    41401 tcaagaccag cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg
    41461 atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct
    41521 aattcatagt catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa
    41581 tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca
    41641 agatctttca ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt
    41701 ttactcaatc ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa
    41761 tttctcatgt aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa
    41821 ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt
    41881 gtcatagaaa gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt
    41941 cctggcgggg aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata
    42001 gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt
    42061 tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct
    42121 tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa
    42181 gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa
    42241 aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta
    42301 cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac
    42361 atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata
    42421 ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat
    42481 accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac
    42541 ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa
    42601 acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt
    42661 catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc
    42721 ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat
    42781 cgaatggaga tgactgccga cacggcgtgc cattacttcg atctctagga aatcttcatg
    42841 tgtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt
    42901 ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac
    42961 acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc
    43021 aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat
    43081 gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat ttatttcaat
    43141 caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata
    43201 atgatcacgc aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg
    43261 ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc
    43321 gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc
    43381 ataaggtaga tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt
    43441 ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc
    43501 agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc
    43561 agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc
    43621 ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact
    43681 gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc
    43741 cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat
    43801 ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg
    43861 aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc
    43921 atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac
    43981 ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc
    44041 tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat
    44101 ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac
    44161 atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt
    44221 gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc
    44281 ataaacgcct aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga
    44341 agtgcctaat tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa
    44401 gattacttga tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat
    44461 aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt
    44521 gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt
    44581 cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt
    44641 ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag
    44701 caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa
    44761 atcgcttcat gacttgccca cgtttttcct gattaccgtt gacacttaag tacatcataa
    44821 cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc
    44881 ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct
    44941 ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt
    45001 tttttgtgtc actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga
    45061 tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga
    45121 tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat
    45181 attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg
    45241 ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag
    45301 ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa
    45361 tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac
    45421 aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc
    45481 acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca
    45541 agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg
    45601 ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc
    45661 agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta
    45721 taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt
    45781 tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga
    45841 ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac
    45901 agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag
    45961 actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga
    46021 attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt
    46081 ttctgattta gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga
    46141 ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct cattagggga
    46201 taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca
    46261 tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc
    46321 ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga
    46381 tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat
    46441 cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga
    46501 agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga
    46561 agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga
    46621 gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca
    46681 caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa
    46741 cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt
    46801 actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc
    46861 gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag
    46921 gccttagatg tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa
    46981 gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc attagatagt
    47041 tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag
    47101 ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg
    47161 gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact
    47221 atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta
    47281 cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa
    47341 aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg
    47401 ccagcgtttt attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct
    47461 ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa
    47521 tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat
    47581 cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa
    47641 tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga
    47701 aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta
    47761 ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag
    47821 gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa
    47881 gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt
    47941 tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta
    48001 ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc
    48061 tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga
    48121 aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac
    48181 gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt
    48241 tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata
    48301 gtgaaaagaa attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa
    48361 attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat
    48421 tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca
    48481 gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa
    48541 tcaacacaag acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc
    48601 tttgatgatg gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa
    48661 aacgtgcatg ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg
    48721 aagcagacag ctgaagcagg gcacgagatt ggcaatcaca catacgatca tcagaatctg
    48781 gccactcttt ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa
    48841 aaagtcactg gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta
    48901 ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag
    48961 acaagaaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt
    49021 attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat
    49081 ctgaaagagc aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct
    49141 gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct
    49201 ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct
    49261 taacaatatc tatacaggta caacaaaaaa ctgtccacca atttt
//
LOCUS       DAZUMO010000014        48933 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000014 DAZUMO010000000
VERSION     DAZUMO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.014"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..609)
                     /locus_tag="ACG8ER_000787"
     CDS             complement(<1..609)
                     /locus_tag="ACG8ER_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049387.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYY"
     gene            complement(620..1288)
                     /locus_tag="ACG8ER_000788"
     CDS             complement(620..1288)
                     /locus_tag="ACG8ER_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5049388.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1418..2002)
                     /locus_tag="ACG8ER_000789"
     CDS             complement(1418..2002)
                     /locus_tag="ACG8ER_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5049389.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2333..2566)
                     /locus_tag="ACG8ER_000790"
     CDS             complement(2333..2566)
                     /locus_tag="ACG8ER_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5049390.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2581..3531)
                     /locus_tag="ACG8ER_000791"
     CDS             complement(2581..3531)
                     /locus_tag="ACG8ER_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS5049391.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3528..4370)
                     /locus_tag="ACG8ER_000792"
     CDS             complement(3528..4370)
                     /locus_tag="ACG8ER_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS5049392.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4372..5091)
                     /locus_tag="ACG8ER_000793"
     CDS             complement(4372..5091)
                     /locus_tag="ACG8ER_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5049393.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5639..5941
                     /locus_tag="ACG8ER_000794"
     CDS             5639..5941
                     /locus_tag="ACG8ER_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049394.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            6013..7239
                     /locus_tag="ACG8ER_000795"
     CDS             6013..7239
                     /locus_tag="ACG8ER_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5049395.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7327..7719)
                     /gene="rpsI"
                     /locus_tag="ACG8ER_000796"
     CDS             complement(7327..7719)
                     /gene="rpsI"
                     /locus_tag="ACG8ER_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5049396.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(7735..8163)
                     /gene="rplM"
                     /locus_tag="ACG8ER_000797"
     CDS             complement(7735..8163)
                     /gene="rplM"
                     /locus_tag="ACG8ER_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS5049397.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(8697..9164)
                     /locus_tag="ACG8ER_000798"
     CDS             complement(8697..9164)
                     /locus_tag="ACG8ER_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5049398.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9284..10822)
                     /locus_tag="ACG8ER_000799"
     CDS             complement(9284..10822)
                     /locus_tag="ACG8ER_000799"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS5049399.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            10902..11588
                     /locus_tag="ACG8ER_000800"
     CDS             10902..11588
                     /locus_tag="ACG8ER_000800"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS5049400.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(11789..12316)
                     /gene="msrA"
                     /locus_tag="ACG8ER_000801"
     CDS             complement(11789..12316)
                     /gene="msrA"
                     /locus_tag="ACG8ER_000801"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5049401.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12400..13077)
                     /gene="pgmB"
                     /locus_tag="ACG8ER_000802"
     CDS             complement(12400..13077)
                     /gene="pgmB"
                     /locus_tag="ACG8ER_000802"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5049402.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13074..14204)
                     /locus_tag="ACG8ER_000803"
     CDS             complement(13074..14204)
                     /locus_tag="ACG8ER_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5049403.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14204..16387)
                     /locus_tag="ACG8ER_000804"
     CDS             complement(14204..16387)
                     /locus_tag="ACG8ER_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS5049404.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(16384..17409)
                     /locus_tag="ACG8ER_000805"
     CDS             complement(16384..17409)
                     /locus_tag="ACG8ER_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5049405.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(17454..18281)
                     /locus_tag="ACG8ER_000806"
     CDS             complement(17454..18281)
                     /locus_tag="ACG8ER_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5049406.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18278..19345)
                     /locus_tag="ACG8ER_000807"
     CDS             complement(18278..19345)
                     /locus_tag="ACG8ER_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS5049407.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(19371..20207)
                     /locus_tag="ACG8ER_000808"
     CDS             complement(19371..20207)
                     /locus_tag="ACG8ER_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5049408.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20204..21073)
                     /locus_tag="ACG8ER_000809"
     CDS             complement(20204..21073)
                     /locus_tag="ACG8ER_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5049409.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21143..22426)
                     /locus_tag="ACG8ER_000810"
     CDS             complement(21143..22426)
                     /locus_tag="ACG8ER_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5049410.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(22440..24125)
                     /locus_tag="ACG8ER_000811"
     CDS             complement(22440..24125)
                     /locus_tag="ACG8ER_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5049411.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            24400..25425
                     /locus_tag="ACG8ER_000812"
     CDS             24400..25425
                     /locus_tag="ACG8ER_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5049412.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26020..29673)
                     /gene="rpoC"
                     /locus_tag="ACG8ER_000813"
     CDS             complement(26020..29673)
                     /gene="rpoC"
                     /locus_tag="ACG8ER_000813"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS5049413.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(29752..33369)
                     /gene="rpoB"
                     /locus_tag="ACG8ER_000814"
     CDS             complement(29752..33369)
                     /gene="rpoB"
                     /locus_tag="ACG8ER_000814"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5049414.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34097..35065
                     /locus_tag="ACG8ER_000815"
     CDS             34097..35065
                     /locus_tag="ACG8ER_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5049415.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35083..35988
                     /locus_tag="ACG8ER_000816"
     CDS             35083..35988
                     /locus_tag="ACG8ER_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049416.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            35985..36791
                     /locus_tag="ACG8ER_000817"
     CDS             35985..36791
                     /locus_tag="ACG8ER_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049417.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            36948..37916
                     /locus_tag="ACG8ER_000818"
     CDS             36948..37916
                     /locus_tag="ACG8ER_000818"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS5049418.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38233..38757)
                     /locus_tag="ACG8ER_000819"
     CDS             complement(38233..38757)
                     /locus_tag="ACG8ER_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS5049419.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39044..40855
                     /gene="scm"
                     /locus_tag="ACG8ER_000820"
     CDS             39044..40855
                     /gene="scm"
                     /locus_tag="ACG8ER_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5049420.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(41010..41996)
                     /locus_tag="ACG8ER_000821"
     CDS             complement(41010..41996)
                     /locus_tag="ACG8ER_000821"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5049421.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(42019..42585)
                     /locus_tag="ACG8ER_000822"
     CDS             complement(42019..42585)
                     /locus_tag="ACG8ER_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS5049422.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(42599..43015)
                     /locus_tag="ACG8ER_000823"
     CDS             complement(42599..43015)
                     /locus_tag="ACG8ER_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5049423.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(43472..45406)
                     /locus_tag="ACG8ER_000824"
     CDS             complement(43472..45406)
                     /locus_tag="ACG8ER_000824"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5049424.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            45708..46643
                     /gene="pplA"
                     /locus_tag="ACG8ER_000825"
     CDS             45708..46643
                     /gene="pplA"
                     /locus_tag="ACG8ER_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS5049425.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            46733..47836
                     /locus_tag="ACG8ER_000826"
     CDS             46733..47836
                     /locus_tag="ACG8ER_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS5049426.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            47849..48802
                     /gene="menA"
                     /locus_tag="ACG8ER_000827"
     CDS             47849..48802
                     /gene="menA"
                     /locus_tag="ACG8ER_000827"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS5049427.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT        14540 a        10120 c         8860 g        15413 t
ORIGIN      
        1 atagtaaaaa cgatgttcag ctataacaat agtaaatcct ttatttttta aatcacaaag
       61 taagtcagca agtttcttag tcgctttgaa gtccaaattt gaagacggtt catccaatat
      121 aattaattta gagtttagcg ataaactaca tccaatagaa attttctgcc gttcaccact
      181 tgatagcgaa aaaatgtttt tatctaatat atttgaaaga tctaataatt cagctaattc
      241 attgattctt ttatccatat gctcttttgt aaatccaaag ttttccatcg aaaatactaa
      301 ctcactcgtt gtatttgttg taaagaattg tcctctagga tcttgactta cgtagccaat
      361 attttttgat atactttctt gtgttaagga ttgtagcgag ttattttcaa ataaaatttc
      421 cccaaccaat tttccgtcat aaagtcttgg aataagtcca ttcattaact ttaatagtgt
      481 ggatttccca ctcccagaag aaccagtaat caatgtaaga ctgccttttt ttattgaaaa
      541 attaaggtca acaagcgctt gttccttaac atccttatat gaaaaagaaa cattgttaaa
      601 ttcaaacaat tctttcacct caaatcaaaa tacttaagaa aaaaatagta acggaaaaca
      661 ggatgatata gtctaataag ctcatgtttg ttttatagta agaacttctt ttcacaggag
      721 attctatccc ctttgttatt gcattagcgg tcatttcttc agcaatcata gttgcacgta
      781 taagaacggg aactaacaga aattccaaat attgcagagg ctgaaaaaaa gacaagcgaa
      841 tgtttctcat ttttaaagat tctttaatca ttcttatttc agaacgataa gtcggaaaga
      901 atctgatcac tactgtaata cttaacatta tttttttcgg taaatgtatt ttttctaaag
      961 aggataataa ttcattgcta gtatagacac taaccaacaa ccatgagatc attacgaccg
     1021 gcaacattcg aaatacaata tataaggatg tcgcaaaaaa tatagtaaaa aactctatct
     1081 tgatagcatc aataattttc attactaaaa acagaactga ataaatcaag atgaaataaa
     1141 tacatgtctt tattttacga aacatcaata ctaaaactgt agcaataatg aaacacccca
     1201 cttgaaatat atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata tttaaagtaa
     1261 tcaaggttaa cggattgatt tttgacactc tttcacctcc aaaaaataag atcgcactgt
     1321 tatcttaagc tgataaaaat gcaccgttat tcagataaca gtgcgatctt atcaaaaagc
     1381 ataactgaga acaattctca atgcaagctt tttttaatta actcagtaat tcaatccacc
     1441 caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct tttgaatagc
     1501 tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag tggatttctt
     1561 cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta tctagacaac
     1621 agactgtctt tttcgtcaca aatgatgtaa ttttttcaac aatatcttga tatttggttc
     1681 cagctgattg gaataaaatc aaatcgaata tacgtttatt ctcaaaaata taattaataa
     1741 atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata ttttctaagt
     1801 ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta atatgcggct
     1861 gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa gcacccgtag
     1921 ttacgcctgc ggtcaggcac agttgcctta tcttagtttg ctgaaatcct tctttttcaa
     1981 aatggcttag tccactttct attaactttt ggtgagttat aatataatct ctggtttcta
     2041 tgggtacttc ctcctaataa gatgaattaa ctttattatc ttacacagga cttgaagttt
     2101 caatcgttaa cgtttattac tgaactcatt ccttagagct aaactaatga gaaagagcag
     2161 aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa tcattcacct
     2221 tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag gataagtcct
     2281 atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc ttttacttaa
     2341 aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga aatcctatat
     2401 ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct ggactaacac
     2461 ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat aattcctgaa
     2521 ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct accctccaaa
     2581 ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg catagtaact
     2641 atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat agataggaat
     2701 attggtatct tttgatactg ttttcattgg tctatcatcc acactgcttt ctacgaataa
     2761 ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat ctggtgttcc
     2821 ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg ctttcgagaa
     2881 atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa ttgaagcaaa
     2941 tttagattta gcttctttgt ctagagaatc caatttttca atatacttat ctagattttc
     3001 tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa tatttttagc
     3061 gtaaataata ccgttttcta aatttaacca agcatgagga tcttccttcc ctttctcact
     3121 ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt ctttgttttc
     3181 ctctttgttc gcatttttaa ctaattttgt aaaccaagca tttcctccag tttccaagtt
     3241 aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt caggcaaagg
     3301 ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa ttttatcttt
     3361 tgctatattt tcggtaatat ctgctaaaat cgaattagta actactactt ttaatttttc
     3421 gttcgaagcc actttatctt tgtctttact atttattgaa catgccccga atataaaaag
     3481 agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca taacgatctc
     3541 tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac aaataagaat
     3601 gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc aataaacaat
     3661 ccaagcaatg atgaacttgc tccgactgtt gcagaaataa atatcatagt tttcaagcta
     3721 tttgcatata aatatgctgt agctgctggt gtaattaaca ttgcaacaat taatatagtt
     3781 cccacacttt gcatagctgt tactgctact aaagttaata gaaccatcaa tagataatga
     3841 taaaaactga cattcatccc tattgctttt gccattaatg gatcaaaaga agtgattaat
     3901 aattctttaa aaaatagaac tactataacc aacactaaaa ccgaaacgcc aattgtaatc
     3961 cacttatcaa tatcttgtac agctaaaata tttccaaata aaatatgaaa taaatccgtt
     4021 gagctatttg caactcctat aaggataact cctagtgcta aaaaagaact gaaagtaata
     4081 ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat aatgatagaa
     4141 gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat ataagaaatt
     4201 gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat tcctcttaaa
     4261 ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt aattagagca
     4321 ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat attattcccc
     4381 ctctccaata aatatagtat ctccataggc tttttttaaa ttttcttcat taaatacaga
     4441 ttctacactt ccgtgagcaa ttagatttcg attcaaaaga ataatattat caaaatactt
     4501 ctttaccttg cttaaatcat gatgcacaat tagcactgtt tttccctgat gtttcaattt
     4561 ttttaacgta tccataatta ttcgttcact aactaagtct atccctacaa atggttcatc
     4621 taaaaatatg aaatccgcat tttgagcaag acatcttgct aaaagtacac gttgaaattg
     4681 tcctccagat aattctccta tttgatgatt agaatatttt cccatatcga ccttttccaa
     4741 agcctttgaa actctttgcc attctgcatt tttaattctt tgaaaaactt tcattccggc
     4801 atatgttccc atagatacac attcttttac ttttattgga aaagtaaaat caatatcagc
     4861 tttttgctct acatatgcaa tcttcaccag ttttttattg atatcttcat tattaatgga
     4921 gacctctccc tgatgaggga taatatttaa cattgcttta agcaatgtgg attttccagc
     4981 accatttgga ccaataatac ctgttaaagc accttcttgt atttttaaag aaatctcgtt
     5041 caatgctaaa aaatcgttat aacaaacatt aagctttttc acttctaaca taactttctc
     5101 cttctaaatg ataatgatta tcacgatcat atggtataca aaaattatat tttagtcaac
     5161 cctaaatttt atagtttaga tttttttctg ttgctgaaga ttagaatgac aaaactattt
     5221 gaaactacgc gtatagtaag tagctttcaa aatatataaa aaaggagcgt tctcaaatga
     5281 gaacatgctc ctaaaactac tttattggcg tatggaaaaa ttatacttga agtggatagc
     5341 gggttgaata agttgataag catcaaaaca tttataaaaa ttattcaaga ctatgtctgt
     5401 tccatataca aatatgaaaa tcagtaaact ccatttaact tttttcgtag tgtgtctttt
     5461 tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa ctatttttga tcattgacaa
     5521 ttaaatacaa tttttgaata acgatccctg ctattcgagc tagaaactca agtacgccat
     5581 gacctgatta gcgacaaata cttagagatt tctttttgtt ctgggaaaac aaaatgccat
     5641 gacaatagca gcaaataatg atacttccgt aaggctcgcc gtaaactggg gagaggtgaa
     5701 attcctatgt gcttacccaa agcaattcaa aaatttcata actactattt taggagatga
     5761 ttttatagaa tccagagtct acctgaataa aaaacaaacc tgtgcatttc taggaatcga
     5821 taataatact ctagataagt ggattcgtat atataatttc ccagtaataa gagttggtgg
     5881 tgtagttcgc tttgacgtag aagatattaa agactttatg aatacattta aaataacttg
     5941 aaattttctt ctcgccgcat atactaaaat tagtgaaata tactgcctta ctttttttaa
     6001 ggagtgagga ttttgcctac aaagctttca aatgggaaat ataaaactaa tctgagatat
     6061 ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa aacatttcca
     6121 aataggcaat tggcgattaa agccgaaaat gatatgaaga agaaaataga aaaagttttg
     6181 agagaggaga atgcaaactc attagaatta aaaggaaaaa taacgtttaa aaaattttac
     6241 gaatctaaat ggcttcctag atatgaatta ggtcaaacaa ttagatcaaa caggcctcct
     6301 agtgatataa ccattagtaa tacaaaagat atatttcggt tgcatatttt acctatgttt
     6361 ggtgaatacg caatgaatta tctaaatatc aatactgaga taatatcaga tgaattaacc
     6421 aaaaaatcga aagaatatgc aaatataaaa atcataaaag gatatgttcg ttccatgttc
     6481 gatattgcag aaatactaaa ttatatcgaa tttaatcgaa ctactaaaat tattcaaagt
     6541 atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa tacaagaagg taagcaagca
     6601 ctatcatcaa aagaacttac taattggata gaagcagtaa atgatgattt taataatcat
     6661 ttacttactt ttcatgatta cacacttttt atgattaccc tgtatcttgg tgatagaaaa
     6721 agtgagactt acgctttgca atggaaatat attgattttg aaaaacaaac ggtccgtctt
     6781 aaacatactt tagataaata tcaaagaaaa aagtttacaa aaggtcgaaa agataccgtc
     6841 attcaagtac cagaagttgt aatgacattg ctaagtgaat ggaaatcggt acaagctgat
     6901 caattgttga aattaaaaat taagcaaacg ttagatcagt atctctttac ttatactaaa
     6961 ccatctggag aagtaaattg tcctgttcat gccgattacc taaactatcg aatcaattca
     7021 attaaaagaa ggcatcctga cttagttcat ttatctccac acaaactccg tcatacttat
     7081 gcaactatag ctagacaagg tggagcagat atgaatcaaa tttcaaatgc tttaacacac
     7141 tcagatattt ctacaacaaa gatatatgtg aacacaccag atatagttga taaggctgtt
     7201 tttgaagctt ttcagagagg attgaataag tgtgattgat atgtgttctt aaatgtgttc
     7261 ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg ccagaaacgt tgatatataa
     7321 aggatattaa cgttttgaga attgtgaagc tttacgtgct ttcttaagac ctggtttttt
     7381 acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag ctaaacggaa
     7441 gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc ctgattgtcc
     7501 agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg tttcagtaac
     7561 agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt cttcaacatc
     7621 ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag caactgcgtt
     7681 tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct cctattagat
     7741 taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt ctgctccacc
     7801 gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa gcataccttt
     7861 tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag cagtgattga
     7921 tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg cttttttacc
     7981 tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat ccaaatgagg
     8041 tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg aaagacgtcc
     8101 caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc ctggtttggc
     8161 catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt gacatacact
     8221 tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta cccgctaatc
     8281 tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt tacttctttt
     8341 ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag aggctgtgac
     8401 aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca actcctcggt
     8461 cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt tcttgtgact
     8521 cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag ctgaccctcg
     8581 gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt tctctaatac
     8641 tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa cagtttttac
     8701 gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg tttttctgtg
     8761 gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc tccagaaact
     8821 tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag tgtttccaaa
     8881 cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt gttatctgca
     8941 aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc ggatacttca
     9001 tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa tgtaaggaaa
     9061 cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg gcttagatct
     9121 gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct tgcctcctaa
     9181 cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca aaatcattta
     9241 cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt tcagaatcag
     9301 ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc ttttgtgtcg
     9361 aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt actccaactt
     9421 gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt ccgatgcttc
     9481 tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg aaaacaagcg
     9541 ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca aacccgcctg
     9601 tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc cgataaaggt
     9661 tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt tgccgctgta
     9721 aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca ccaagtaaat
     9781 agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc gaaagcagtg
     9841 catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt aaaaatgtat
     9901 gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact acccaatgtt
     9961 tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc tgagtagtaa
    10021 taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca ccaaaagcac
    10081 ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat atatcatttg
    10141 gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag gtggaaggaa
    10201 agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct tctgcccatt
    10261 ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg ctgatcagtt
    10321 cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca gtttttttga
    10381 ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc attggatgaa
    10441 tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc agtttttctg
    10501 cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt gttttctctt
    10561 catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga atgattgtgt
    10621 ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga aagatcaatt
    10681 cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga aaataagcgg
    10741 atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg gttccgatat
    10801 ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac aaaaatttag
    10861 tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt tattttatca
    10921 ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg ttaggtcact
    10981 ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct tggtatgaat
    11041 tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc aggtgtcggt
    11101 tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc tggttcttta
    11161 ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct ttgctatttt
    11221 cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt tcttgggcaa
    11281 ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg ctgttaacgt
    11341 ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct ggcgatactc
    11401 tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag atcttgtttc
    11461 ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat aaaaaaaagt
    11521 tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc cttcatctta
    11581 ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat agtgttctct
    11641 taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca ctaaatggtg
    11701 ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct ctgatatacg
    11761 gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt cgacgaagga
    11821 aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt ttcttgtaat
    11881 aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt tttgtcacga
    11941 ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct gcaatatttc
    12001 gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga tcagcgaact
    12061 gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac tgatcataag
    12121 aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt gtgcctgttg
    12181 ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat gtcacagaaa
    12241 tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg cctgcgaaaa
    12301 ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat ttccaaaagg
    12361 aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg atacggcttc
    12421 tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa tcacatattc
    12481 agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac cagccaaagc
    12541 tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg gtacattcaa
    12601 ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata cttgcgtgcc
    12661 gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga ggcgtgcatt
    12721 tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact caagaacagc
    12781 tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa gatatagatt
    12841 gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt ccatttttcc
    12901 aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt gctcgttttc
    12961 tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt cgtaatgata
    13021 acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga ttttcatcga
    13081 ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc tgtgcgatga
    13141 agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg aaggatcgga
    13201 aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac acttccgcaa
    13261 atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg cgcttttcct
    13321 tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc ttgctgtacc
    13381 cgtttatcga actccagtaa atccaatacg tagtcgatgc cagagactat tttccctgaa
    13441 gcaaatgcta acagtcctgc acccattcca ccaccagcac tactgcccga tgcctcgatt
    13501 acttgagggg caaatctcat ataaaaatca tgcatggctt ggtcagccac tgctaattca
    13561 ttttctttca agcctttttg tggtccgaaa acaaaagaag cgccttcttt tccgcataaa
    13621 agattagtaa catcagtaat gacagtgatt ttggctttaa tcagctcaat aggcacgttc
    13681 gttttatcaa ctgtagcaat tttacctaga ttttctccca gtgcttctac tttttgatta
    13741 tcttgatcgt aaaaagcata acctagacct tctgccatgc cgatgccgcc atcgttggta
    13801 ctacttccgc caacgccgat gatgatgtgt cgaacacctt gttgcaacaa ttgtcgtatc
    13861 agttctccta ctcctcgcgt ggataaccta agtggctgtt gttggtttag tgggacatct
    13921 ttcaatccga cgatttctgc catttcaaaa atcgcagttt gaccgtctga agcgtactgg
    13981 atttctttgt ttttgccaaa tgcattagta acgactgctg tttgccgctt caaccttaaa
    14041 ctgtctgcta atgatgaaac cgttccttct cctccatcgc ctataggtag cagatcatat
    14101 tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc ctctaaggct
    14161 gtcagacttt ctttgaaaga atctggcgca atcagaatac gcattatttt cccctccgtt
    14221 ccaagatcag ttctttttcc agctggtatt ggtctccatt gacaaaaata ggtaatgttt
    14281 ctttcgtctc tttttttatt actatttgtt tttttgtcaa aacaaatagc aacctgtgat
    14341 ttttccaaat aaaaggaaaa gaaatttttt cccatgctga aggaagtctt ggagaaatag
    14401 aaagagttcc attcgttact gtaatacctg aaaaaccaaa tataactgct agccatagtg
    14461 ctcctaatga agcagcatga attccgtcat ctgatgaatg cggatctggt cctaaatcaa
    14521 tcaggcacgc ttcttggaat aaatgatacg cccattccat ttcttggata cggcaagcct
    14581 ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat tgttttgttt tcatagtaat
    14641 gcaaattctt agaaacgatt ggttgagaaa aatactcagg taataagtat aacagcatga
    14701 caagatctgc ttgcttgctg acttggagtt catttacttc gctacgggca taatcaagta
    14761 aaatagactg gctgccagga cgttctttgt aagtggtcaa atcaataact ggcttactga
    14821 gatacgtgtc atcttgcgga atcagctgtt ctttgttagg cactggcaaa tataacttat
    14881 ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat ttaacagcta
    14941 aggccacatt ataatgcgcc agatagttcg tatatgcatt attgtcgata tattctgtgt
    15001 attcatctgg accaatgact ctgtgtatct ctaaatgatc atcgactttt acagctctgc
    15061 tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc ctttgtttct aaaaacgatt
    15121 gatcctctgt tgcttggaca tactcttgaa cggcataagc aaggtctgca ctgatatgat
    15181 gttctgcggc agctgaagca acttcttgtc ttgttccggt tttgatattc aatgcagcaa
    15241 atttgggtgt ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt gcaccttgat
    15301 agtgataatc tcttgctttt tgccgtgcgc cttctaatcg atcataacgg tacagcagca
    15361 aattctttgc tacttcagga aactgatata aaaagaaagg caggataaaa atttccgtat
    15421 cccagaaaac atgcccttta taaccttcgc cagttagtcc cttcgcccct acactaacac
    15481 ttggatcatt gcggggagtc ataatctgta aatggtaagc agcgaagtcc atcgctaact
    15541 gatcgaaaga agaatcgcct tggatcgtta tccgatgctg atgccaaaaa tctgtccaat
    15601 aagacatcga ctgctctaaa atttctgtat aactaacgct tttctgggct tgggcaatac
    15661 ttttcttaaa cgggtctgtt tgctgaagag aggtagagag cacgatgatc ttctccagcg
    15721 taaaggacgt attttttctg atcttttctg aaaaggaagc ggtgatccgg cgattttttg
    15781 cttggaatac acctgcttta ttgaatttag ctgcaatccc aatatcaatt ttactttctg
    15841 ttgtctggta ggatgcactg atcaaatcgt caccaaatac tcgcagttct ttttcaacca
    15901 actgctgtgt accaaaattg gtttgctgtc catcgatacc cgttttcaca tctacttggc
    15961 agtcttcggt caacggcttg atgaccactt tcagggctgc ctgatggaga ttattttgat
    16021 cagcaacacg acaagtaatg acttccactt tttttccttg agcagtttca atgagcgcat
    16081 agcgtttaag ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga actttcatcg
    16141 tctcaagaga aaaatgttca ccgtcgattt ttatttccca tctgatgaca tctgggagat
    16201 tcactaactc ggctgattct gctcctaaag gtcggttata tacaccagct gcaaacattc
    16261 cccttacttg attccgatag tcttcctcct ggcttcctct gataccaaaa aatccatttc
    16321 caatggtcat ctgactcgca tacttattga tatactctaa atcaaactgt ttttcttcta
    16381 ctttcataag gtaataactt ctcctgtttc tgccgaacga tataaatttt ccaccagatt
    16441 ctggatgaca tatccttgcc agccatctgc aatcatctga gtctggtcac cgagcgcccg
    16501 attcacaaaa tgagccatac ttagttcatg tgggtcatta actggcgtgg tcttttgtaa
    16561 ggaaaccagc tttccatcta catcttcgaa tagttcgcct ggatacagat tagcacctgc
    16621 ctgatcgcca caaaaagcca cattcagttt gtcttctctg atattcaaag caaacgaagt
    16681 attcagttgg atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg catcttctac
    16741 tgtgaatttg cttggatccc atttcccgaa tttccctgaa ttttttttcg taccaatttt
    16801 ttggaagctg tacgcccgaa cgctttttac tttaggaaat ccagtaatat acatcgctgt
    16861 atcaagcatg tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt ctttgtcgat
    16921 aaacgttccc cagcctggaa caccagaccg acgaagcgca ttcgcttcta cgtaataaat
    16981 atccccgaga acatcaagat gattcttgat cattgttgct tcgtcagaaa aacgatgctg
    17041 gaatccataa gccaaaatct gcccgttttt atcggctgtc tctgccatct ttcttgcttg
    17101 atcagctgtc atagctggtg gtttctcgca catcactgaa gcattagctt cgagggcagc
    17161 caatacatgt tcgaagtgaa agcgattggg tgtacagaca ctcacgatat cgggagaaat
    17221 ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac gtcggcaaaa
    17281 gtcttctgct cgctggctga ctggatcaac cacagcggcc aattctaaga catctgatct
    17341 tttttgataa taagcagcat gtactttttc tgctacttgt ccagcgccta tgattgctac
    17401 tttcttcatt tatttctccc tcctttgctt atttacttat tgttattctt tctctattct
    17461 ttctctaaat catttaaatg acgcgctgtt tccagacagt ttttcaagta ttgcaatgat
    17521 tcctgataaa ttttcacttc attttctcct cgcaaacgac attcatatac catataacct
    17581 tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc gccagatcct
    17641 ggttggtagc gatgattatc tgctaaatgg atatgaccca catactcttt ttgcgtagtc
    17701 aatgcttttg aaatatcatc ttcttcaata ttcatatgat aaaaatcagc gatgatctgc
    17761 acatgtttca attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa attgatcata
    17821 tggtcttggt atcggttcaa aggctccaaa taaataaccg tacctgtttc ttcggctact
    17881 ttatctaaat aacgaagtga ttcgctgact gctttatagt ctccttcttt gcttcggggt
    17941 gaaaccatcg gcggtaatcg ataagtgaac attccccaag ctgctgggat aacgattcct
    18001 ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac accattaaga
    18061 cgccgttctt cgataaagtc accgatccac ccatcgtagc cgttacaagc tgtcactact
    18121 gggatccctg tttctttgat tgcctgtttg actgtgtcga tattttctat taacaactta
    18181 ccgtcgattt cgtattcttc aaatccacag tctttgataa atctaaattt ttctaaaatg
    18241 tctcttggga aaaatgcttg atcttgcgta ccgaatttca ttgttctcct cctcggactt
    18301 gaacgcccat tttgatactt tcttctgggt gctggtctac ataacgcatg taagcttctg
    18361 cgctttctgc aaatggcaca actggatcaa tgatttcttc acaatccaag taaccgttca
    18421 tcaataattc ccaacatgtg tcctcgattc gtttacggtt ccatcttggg taatctgggt
    18481 ttggttcact agatgctcga gcaaagacga tttttgcatt attgaaatgt gcttcgcgac
    18541 caagattaaa cccagctgga aaaggtttag caaaggcaac ataagaaatg atcccgccat
    18601 agccgatccc tcttaaagaa gcttgtaatg cggcagcact gccactcgtt tcaataactg
    18661 aatctactcc acgtttatct gtcaattcct ttagacgaaa accgacatct gctttcatag
    18721 gatctagcgt gtaatcggca ccgtattttt ctgctaattc tctacgctgc gctatgggat
    18781 cgatcccaac gatggtagta gcacctgctt ttttagctaa ttgaatcgca atttgcccga
    18841 tagcgcctaa accgaagatt gctacagcat cacctgctcg aacatgtgct tcccggatac
    18901 cacctaaagc gaattgagca ggatcgtaac atactgcatt tttccattga tccaaccgat
    18961 caagtttacg tagtttataa ttatcaacac cgtttgtaat cagcgtttca cgaatgggtc
    19021 cataagtgca gactaactct cctttttgat actctgtcac caaactgccc gtttcttcga
    19081 tcgtaccgac aatcatattt cctaattgaa attcgccaaa taccacaccg cgttggcttc
    19141 cttcttctct tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg ataaatggac
    19201 ttaatcctct aaagtcgaca atttctgttc ctggttttgg agtagcgaaa attactcgga
    19261 tacgtacttc gttcttttcg atctcacggt cttcgtaatc tttgaatccg gctattcttg
    19321 gttctaatgc aactaatttt ttcatctttt ctttcctctt tcttttcttt ttatcctttt
    19381 acgccgcctt cgactaaatc acctttaata aatcgttcag ataacgcata cataatgatg
    19441 actggtaaag cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta gtctggtgta
    19501 ctgaataatg tgttcaaacc gattggtaaa gtaaattttt cggaggtcga caggaaaatc
    19561 gaagcaaata aataatcatt ccacgaaacc ataaagcaat aaacgaagac agaaatgatt
    19621 ccagaaatag ccaatggtat aatgatccga ataatgatct ggatccggct aagaccatcg
    19681 atcattgctg cttcttcaat atcagtcgga atcgtgtcaa aataactttt tagcatgaat
    19741 atcgcagtag gaagtgtttg aacgaccatc gtgataatca gtgctgtatt ggtatcatac
    19801 aaacctagac cggaaatgat tttaaataat ggaacaacga tcaaaatacc ggaaaacata
    19861 taaacggtgt aaaagctagc attgattttc gagcgagctt tgaagcgtag ttttgacaaa
    19921 gcgtatgccc cgctgatccc aatcactact gcagttacgg aggctatcaa ggagatcact
    19981 aaactatttt tgaaatacat gatgaatgga aaaatctcag cattgaaaat atcctgataa
    20041 tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc ttcttgtgaa
    20101 tccttgaatg atgtcatcag catgatcaaa aatggaaaca aattgacagc taatagcaac
    20161 aaaatcgtcg cgtaaaataa gattttcctg ctctttttat ttttcatttc aagtccacct
    20221 tctttcgtga gagcaagacg accgctgcaa taatgatgaa caatagaatc gatatagctg
    20281 ctgcttttcc aagatcattg aacgcaaagg ctgtctgata caaataaacg cctaagatat
    20341 tgactttatt cgtcagtaga tagacatctg taaacatata gaacatccag atagatcgta
    20401 aaacaatcac agttgctagt actggtttga ttgccggcag tgtcacgatc ttgaacttct
    20461 gccaagcatt tgcaccgtcc atttcagctg cttcataaag cgttttgtcg actgtttgca
    20521 aaatagccaa aaatgaaata aacgcatacg gatagtatct ccagatagca aacaaaacga
    20581 ccataaagaa gctagcaact ggctggtcga accacagcgg tgcttcgttg aaaagctgca
    20641 acctatctac taacagatca ttgacgattc cataactatt attgaacata tatttccaag
    20701 caaaaatcaa agaaatagat ggcgtaacat aacttaataa aatcagtgaa cgcaccgttt
    20761 tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa gctgtactgc
    20821 ctgctacaac taagaatgtg taagcaactg ttaaacctaa agaacgataa aattcactat
    20881 cacttaaaat ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag ttaggattca
    20941 gcggcatatc gaaaaaactg atctgaatat tggaaagcat tggaatcagc actaaaacag
    21001 ctaataaaag catgcttggt gctaaaagtc ccatggctaa ttttaagtca gaatgtttcc
    21061 ttggacgatt cattgaaacc tccccctgat aatctgccaa tagctatcta cttttttcga
    21121 acaccattgg cagaacttct tgttatttcg tatttttttg tgcgtcagct aaagcttgat
    21181 cgacatcttc gtttccgaca gttacttggt tcactgcttt tccgatcaca cctgagcttg
    21241 tgatatctcc cattttcgtg aaattctttt ccccgactag tccaaagact tgtacttcat
    21301 caaaagcact agcaatctca gatggcagct cgccaaaaga cttgatgact tcattttctt
    21361 tgtaaccatc cagttcgact acttgtttat tcactggttg agcaccacca ggagacatca
    21421 agacccactt ttccatgttt ttcggttgag ataagaaagc tgtgaatgct tcagcagcct
    21481 ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg ccatagacag
    21541 cttgatcttt ttgtgttggg atagcaaaac cgatattttc tgagttcccc gcttcataaa
    21601 cagaaggcaa aatgtaagtt gaatagattg ccattggtgc tgttccattc atgaaagcat
    21661 ctttgatttc tgtcacatca ttagagcctg gcatcgtgta ttgggataag ttttgataaa
    21721 acgacaatgc ttctctcatc ttttccgtat cgatcgttac ctcgccttca tcatccaaaa
    21781 catttgctcc atttgacaaa gcaaattgag agaacgcttg ctcactcatt gttccttcag
    21841 cagtaggcat agcaattcca tattgtttat tctctttatc tgtgaaatgt tgagcaattt
    21901 ttaaaatatc atcccaattt tctggttctg aaaaaccagc ttctgataat ttttctttgt
    21961 cataccaaat accttgaacc cagccgctaa ttggtgcagc aatatagctt tttccatctt
    22021 ctgaacgaac aaggtttttg gctccgtcat aataattatc ttctccagct tcctcgataa
    22081 cgttttggac tgccgtctga tcaatcagtt catctttatc catcacttta gcaaaatcct
    22141 gactgacttc cattacttct ggtaatttac cggaacgagc taacgtaacg atttttgtat
    22201 tgaacgctga ttcttctacc ggcacttgct tgattttgat gtctggattt tctttttcaa
    22261 aatcagcaac cagttcattg atcacactta atcgttcctg ttcgacagaa gagtgcatga
    22321 attgaatcgt tgtctgttgt gattcgttag agctttgact ttcaccacaa gcagtaagaa
    22381 gaaatccagt agctagcaga caggtagtaa caataatttt tttcattttt ttccacctct
    22441 tattttatta ttttcagcca catatattca tacggctgga atatcaactt tcgttcttga
    22501 ctgcaatgga taatttgtcc agaaaacagg tctaggtaat ctccttttgg taaaagataa
    22561 cctgctgatt gattggaaaa attatggata actaagactt tctcttcctt cgcttcgcga
    22621 ataaaactga taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt cggatggaag
    22681 gctttttctt tttttcgaat agatagcatc tgggtcaatt caccatatac tgctgttctt
    22741 aatggatctg tctgcaactc tttctcaata tctgctaaca aaaatttttg ccgattgatt
    22801 tttcgattct ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc cagcacactt
    22861 tgaatataaa ttgccggcac gcctggtaac gttaccaaaa atgaatgagc agctaaaaag
    22921 cgtttgcttc tccattccgt cgtatctgtt ggagaactta acgcatctag atacgtagcg
    22981 tttatctcat atggactggt cgttccatct gggtttttct tgtagtttac tcgggcaccc
    23041 tctttcttca gctgttccac taattcaagg atcgtttttt ccggcagaat acctcgtacc
    23101 ggattcaatc cgataccgtc atgggaggct aaaaaattaa aaaaagttgt ttttttcgat
    23161 gcgaaatcga tggtttttaa ccattctttc aacttacttg tattacccga acgaatcgct
    23221 tgaagggtca aaggtggtag cgaaaactga tagaccatct gtgcttcatt ctcgccattc
    23281 ccaaaataac tgatattatc tttgtgtggc acatttgtct cagtgatcaa taccgttcct
    23341 ggagctgctt catcagcgat tgcacggaat aattggatga ttttatgcgt ttctggcagg
    23401 tgtatacaag aagttcctag ttctttccat aaaaatccca cagcgtccaa gcgaataaat
    23461 gaagcgcctt tttctaagta aaacaaaaga atatcgatca ttttcaataa aacttctgga
    23521 tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg tttttctttc
    23581 ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc ttggtctagt cacgctgctt
    23641 gtatctgtat ccagtggcaa atcaataaaa aaatctttgt acgtatcatt caagtttaaa
    23701 tattcctgaa accaagtact ttcggcagac atatgattgc agacaaagtc gaacattaac
    23761 cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc ccaaacggtc gttgattgcc
    23821 tgataattta caacggagaa accatcatca gatgtatatg ggaaaaatgg caagaaatgt
    23881 acaactctaa atgtatctgc caaatacttt tgataaaatt tcttaaatgt acgcaaagtt
    23941 ggttgctgct cttcaaaaaa ttgatcccca taagtaatca agaaaatatc ttcttcgtcc
    24001 caatgcagtt tgcctgcctg gcctttcttc tgctgataaa cactgacttt ttgttcaatc
    24061 atagaggcgg ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa aagttttgta
    24121 tacatcggat tcccctttct tttttgggag cgttcccaac gatttgatca tattatggaa
    24181 aacgcttact gtcaatagca tttttttaaa taattttgat atacacaaaa atacaaataa
    24241 gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa tatggtctct catgatcatg
    24301 caagctattc ctaaattttc aaaatcatag acaaaaaaga atgtctattg tactatggaa
    24361 ttattctaaa acgctcccaa aaaggaggtg catataaaat tgaaacctac tatttatgac
    24421 attgcgaaaa aagctaatgt ctcaaaatca actgtctcca gagtattaaa taaccagcca
    24481 aatatctctg ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt gaattatgaa
    24541 ccgagcaaaa ttgcaagagg actgtcagga aatggttttg acgcgattct agcattatct
    24601 catcgtactt ctcatacgac ctatggcaat cctttttttt cagatgttat tcaatccatt
    24661 tctgccgttt ctgaacaaaa taacttcgac ttgattctac aaacggcaaa aacaccagaa
    24721 gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg aattattatt
    24781 ttaagctcta cgatggatga aacacttctt tttgaattgg atcaatacaa tattcctatt
    24841 gtggtaatcg gaaaagtcgg aaacgactac acgaacatct actcagtgga cacggataat
    24901 ttccaggata gttacgattt ggtggaaaca ctgatcaaga atggacaccg tcatatcggc
    24961 tgtctccatg ctccgacaga agtttatgta tccaatgatc gtctcaatgg ttaccgtcaa
    25021 tgcttatttg atcaccacct agatatccgt aatgaatgga ttatagacgg cggctttcgc
    25081 ttagaagata gcatggcagc aatgaatgat ttaatgaagc tacgagaaag accaactgcg
    25141 attttttcta ccgatgcatt aaaatcattg agtatttaca aagtggcaga acaatctgga
    25201 aagcagatac ctgatgatct ttcgatcgca ggattcagtg atcccttatt gtcctcccta
    25261 ttttcgccac agttgactcg gatagacatt ccgacaaatc agttaggaat caaagccaca
    25321 gaacttttat tccaactaat ccaccaccaa taccctgaag aaaaaagaat cattctttca
    25381 accgagatta ctctcacaga ttcgatccaa caacaagaaa tgtagaacag aattataaaa
    25441 gaacagaaac actgaggata agtcagttac tctgttcttt tttcaatcac tcatccctcg
    25501 ttaccccctc ccggtatttt agcgaatttt tcttattttc catctgtttt agatagctgt
    25561 ggataactat aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa actattttta
    25621 accacgttgt gtataagttt ttatagttcg tttttttaaa cacatttacc cttatttcaa
    25681 tccatgttat ttggggatac ttatctctaa aacataaata gaataacaga catgtctact
    25741 ggttttgttt tattcgagga attattcaat aatagctagc aaaatattaa aaataaccgc
    25801 tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac ttttgttgat
    25861 tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc gatgttgtta
    25921 agatcctttc ccacttttct tctagttaga caaattattt tagacaaacc aaaaaagagg
    25981 ttgtgacaat aatttgtcac aacctctttt ttaaaatatc tattttgttt gattgttcat
    26041 tgcgtcttct gcagccattt gcgcttcaat atctgaaata ctgtagacat tttcgcttgc
    26101 tacaccgact tcttttggtt ccatgtcacg ataacgttgc ataccagtac cagctgggat
    26161 gatcttacct ataataacat tttctttcaa accaagaaga ggatctttct taccgcggat
    26221 cgcagcatcc gtcaagacac gagttgtttc ttggaatgat gcagcagata agaaactatt
    26281 tgtttctaat gatgcttttg tgatacctag taagacaggt cgagaagttg caggaacgcc
    26341 tcctgagact agcgtattgt agttttgatc tttaaagtct gcgatgtcta ataatgtacc
    26401 tggtaagata tctgtatcac ctggatccat tacacgaact ttacgcagca tttgacgaac
    26461 cattacttcg atatgcttgt cgccgatttc taccccttgc atacggtaaa cacgttgtac
    26521 ttcacgaagc aagtagtttt caacagataa tacatcgcga acttgcaaca attgttttgg
    26581 atcgattgat ccttctgtca atggtgcacc acggtggatc atatcgcctt ctgctacttt
    26641 catacgtgct gtataaggaa cagtatacgt acgtgtatct gtttttcctt tgattgttac
    26701 ttctttggta cgtgttgcag gatcttctga gatatcgatg acatcccctg ttacttctgt
    26761 gatcactgct tgccctttcg gattacgtgc ttcaaagatt tcttggacac gaggaagacc
    26821 ttgagtgata tcgtctccgg caaccccacc tgtatggaat gtacgcatcg tcaactgagt
    26881 accaggttca ccgattgatt gagcggcgat cgtaccaact gcttctccga cttcaacgtc
    26941 agagccagtt gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt ttgtattaca
    27001 tgtgaagaca gaacggattg tcactgtttc gacacctgca tcaacgattt gttttgcgat
    27061 gtcttcggaa atcaattggt ttcctgcaat gatcaattca tttgtttcag gatgacgtac
    27121 ttctttacgg gtgtaacgtc ccactaaacg atcttctaat gattcgatga tttcatttcc
    27181 ctcgcggatg gcttggattt caagacctcg gtcagtaccg caatcttctt cacggatgat
    27241 tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg cagtcttcaa
    27301 ggctgtatcg gtcatccctt tacgagcccc gtgagttgag ataaacattt ccaagacgga
    27361 tagtccttca cggaagttcg aaatgattgg caattccatg attcgaccat taggagcggc
    27421 catcaaacca cgcattccgg caagctgtgt aaagttggaa atgttaccac gagctccaga
    27481 atcagacatc atgaagattg gatttttcgc ttccaaacct tccatcagtt tttgttggat
    27541 gctgtctttt gcagcattcc aaacagcaat gacacgttca taacgttcgt catctgtgat
    27601 cagaccacga cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat tttcaatgat
    27661 ttcttgtttt tcattcagta cactgatgtc agcgatccct actgtgatac cggcatgtgt
    27721 cgaatgtttg taccctaagt ctttcatacg gtcaagcatc ttagaagttt cttttacttt
    27781 gaatcgtttg aagacttctg cgatgatgtt tcctaagttt ttcttcttga acggtaatac
    27841 taattcttgc tctttgatat gtgcaggaat atcagtacca gcttcaacaa agtatttgtc
    27901 cggtgtttgg acggtcaagt tgaagtctgt tggttcattc agataaggga attcaggagg
    27961 catgatctcg ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt ctttttgcca
    28021 ttcagtaaat ggtttttcac ctaatgagtt agggttcaca ccgatacgtg aatgtaaatg
    28081 aacatatccg ttgcgccatg cgataacggc ttcgttttga tctctgaaga ccattccttc
    28141 gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta cctagaacca tatcttgaga
    28201 aggagtaaca actggtttac catctttagg gttcaagatg ttttgagcag ctaacatcaa
    28261 catacgtgct tctgcttgtg cttcttcatt caatggaacg tggacagcca tttggtctcc
    28321 atcgaaatcg gcattataag cttcacatac aagtggatgc aaacggattg cgcgtccttg
    28381 tactaaaact ggttcgaatg cttggatacc aagtcggtga agcgtaggtg cccggttcag
    28441 caatactggg tgttccttga ttacgtcttc taaaacatcc catacttcgt cttcttgacg
    28501 ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa caagctcacg
    28561 catcacgaat ggtttgaata attcaatggc catttctttt ggaagtccac attggtacat
    28621 cttcaagaaa ggtccaacaa cgataacgga acgacctgag tagtcgacac gtttacctag
    28681 caagttttga cggaaacgac cttgtttccc tttcaacata tgagaaagag atttcaatgg
    28741 gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca atgcatctac
    28801 tgcttcttgc agcatacgtt tttcattttg aacgatgatt ccaggtgcat tcaagtctaa
    28861 caaacgtttc aaacggttat tacggttgat tacacgacgg taaagatcat tcaagtcact
    28921 tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt aaatctggtg gaataacagg
    28981 gataacgtcc ataaccatcc agcttggttg atttcctgaa gcgcggaatg cttctaaaat
    29041 atccaaacgg cggattgcac gtgtacgttt ttgtccttgt gcagatttca attcttcttt
    29101 caattcggct gcttctgcat ctagatcaac attatcaagt aattgtttaa tcgcttctgc
    29161 acccatagca gcttggaatt cttgtccata ttgttcacgt ttttcgcgat attcgcgttc
    29221 tgtcaacaat tgttttttct ctaaattcgt atttcctggt tcgatgacta catatgaagc
    29281 aaaataaatg atttcttcca aagcgcgtgg gctcatgtct aatacaagac ccatacgtga
    29341 aggaatccct ttaaagtacc agatatgtga aacaggagct gctaattcaa tatgtcccat
    29401 gcgttcacgg cgaactttcg aacgagtaac ttccactccg catcgatcac aaacgattcc
    29461 tttataacgg atacgtttgt attttccgca ggcacattcc cagtcttttg taggaccaaa
    29521 aatccgctca cagaacaacc cttcgcgttc aggttttaag gtacgatagt taatcgtctc
    29581 aggttttttt acttcaccgt aagaccagct tctgattttt tcgggagaag ccaaacctat
    29641 ttgcatactt tcgaatttat ttacatcgat caaaaggggt tccctccatt tcatttaatg
    29701 gcaaaccgca agacggctga tcttcgaccg ccttgcagct catagaccgg attagtctaa
    29761 attatcttct gcggtttcaa agttttggat gacatcgtct ttttcatctt ccacttgttc
    29821 agcagctttc ttttctaatt ctttggcagt ttgctgttcg gcaaattttg tcaaagcatc
    29881 aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct ctgtttcttc
    29941 gatatccaag acacgcatgt ccagacctag tgattgcaat tctttcacca atacgcggaa
    30001 tgattccggt acacctggtt ttggaattgg ttcacccttg acgattgctt cgtatgtttt
    30061 cacacgtccg actacgtcat ctgatttgta tgtcaagatt tcttgtaaag tataagccgc
    30121 accgtaagct tccagtgccc atacttccat ttcaccaaaa cgttgtccac caaattgagc
    30181 tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac gtgcatgcaa
    30241 tttgtcatcg accatgtgag caagtttgat catgtacatc acaccgacag acacacgtcc
    30301 atcaaatggt tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc tggccatacc
    30361 ggcttcacga actgtttccc atacgtcttc atcacttgca ccatcaaata ctggcgttgc
    30421 tacgtggata cctaactgac gtgcagccat acccaagtgt aattccaata cttgcccgat
    30481 gttcatacgt gaaggcaccc ctaaaggatt cagcatgata tcgattggtg ttccatcagg
    30541 taggaatggc atatcttctt ccggcataat acgggataca acccctttat ttccgtgacg
    30601 accggccatt ttatcccctt catggatttt acgtttttga acgatataaa cacgaactaa
    30661 catgtttacg cctggtgaca attcatctcc agcttcacga gtaaagattt tcacatcgtg
    30721 aacgataccg ccgccgccat gtggtacacg taatgatgta tcgcggactt cacgagcttt
    30781 ttcgccaaag atcgcatgca atagacgttc ttctgcagat aattctgtta ccccttttgg
    30841 cgtgactttc cctacaagca aatcgccatc ttggacttct gcaccaatac ggatgatacc
    30901 catttcgtct aaatctttta atgcgtcttc cccgacatta gggatttcgc gtgtgatttc
    30961 ttcaggtcct aattttgtat cgcgggcttc tgattcatat tcttcaatat ggatagaagt
    31021 gtatacgtca tcttttacca aacgacggct catgatgatc gcatcttcat agttgtaccc
    31081 ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc cttgttccat
    31141 agatggtccg tctgccagag tatccccttt ttcgactttc tcacccaaat gaacgattgg
    31201 acgttggttg tagcttgttc cggagtttga acgacggaat tttgttacat cgtatttatc
    31261 taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt caacgacacc
    31321 atcatgttta cataacaaag cagctcctga gtcatgcgct gatttgtatt ccataccagt
    31381 accaacccat ggtgcttgag gattgatcaa cggcactgct tgacgctgca tgttggcacc
    31441 catcaaggca cggttggagt catcgttttc caagaaagga atacatgctg tcgcaacggc
    31501 aactacttgt ttaggtgata cgtccatgta gtctacttta tcaatagata cttctaagtt
    31561 ttcactttga gcacgagcca taacaacgtc ttgagcaaat gttccatctt cattcaatgg
    31621 gctgtttgct tgggctacga tgtaatggtc ttcgatatca gctgtcaagt agtcgatttg
    31681 gtcagtaaca cgacctgttt cacggtctac acgacgataa ggtgtttcga taaatccgaa
    31741 tttatttact ttcgcataac tggacaaact attgatcaac ccgatgtttg ggccttcagg
    31801 agtttcgatc ggacacatac ggccatagtg ggagtagtga acgtcacgaa cttcataacc
    31861 ggcgcggtca cgagtcaatc cgccaggtcc taaggcgaaa agacgacgtt tgtgtgtcaa
    31921 ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac tgagaagaac caaagaattc
    31981 tttgatactt gccacaactg gacggatatt gatcaattgt tgaggtgtca atgtttcagt
    32041 atcttggatc gacattcttt cacgaaccac acgttccata cgtgctagcc cgatacggaa
    32101 ttggttttgt aaaagttcac cgactgaacg gatacgacga tttcctaagt ggtcgatatc
    32161 atcgacattg ccgatacctt ccatcaagtt caagaaatag ctcattgaag caatgatgtc
    32221 ggctggacga accgttttta cagctgcttc tggatagccg tttccgatca cgtttacttc
    32281 acgttcaggg tctcttggtg aaaatacttt gatgacttgg acagtcattg gatctgtgac
    32341 cacaccgtct tctgatggat agtaagtcac tgaatttagg ccattatcaa tgaaaggagc
    32401 tagtgtttcc atgacttggt gagtcagaac tgttcctttt tcaacgatga tctcgcctgt
    32461 ttctggatca actaatgttt cagctaatgt cagattcaat agacgtgttt tcaaatctaa
    32521 ttttttgttt actttgtaac gaccaacgtt tgctaagtcg taacgttttg gatcgaagaa
    32581 acgagcattc agcaagttac gtgagctgtc tgctgttttt ggttcgcctg gacgcaaacg
    32641 ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt tatgcaagtc
    32701 tttttcgact gtattgcgta atgtttcgct gtcgccgaag atttcaaaga tcgtatcatc
    32761 ggaaccaaat cctaacgcac gaactaatac ggttaaagga atcttacgtg tacggtcgat
    32821 acgtacataa gagatgtctt ttgcatcagt ttccatttct agccaagctc cacggttagg
    32881 gataacagta gaaccgaatc cttcttttcc gtttttgtcg acttttccat ggaaatagac
    32941 acctggtgaa cggactaact gagaaacgat tactcgctct gccccgttga tgatgaatgt
    33001 tccttgttct gtcattaatg ggaaatcacc gaagaatact tcttgtgctt tgatttcgcc
    33061 agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt gcagagtagt ttgcatcatg
    33121 cgcacgagct tctgctactg tatactttgg ttctttcaat tcataatcta caaattccaa
    33181 agatagattg ccattgaagt catcgattgg caaaatgtct tcaaacattt ctctaagtcc
    33241 ctcatctaag aaccattggt aagagtctgt ttgaatttcg atcaaatttg gtaattccaa
    33301 cacttcactg attcttgcga aacttctacg ttcgcgatgt tttccgtatt ttactacgtg
    33361 tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa agactacccg atcaattgtt
    33421 acaataatat ttcaaatatt atttttaggt aacaacagac attttttgcg gcttttaggc
    33481 aaaaaaaaac caaaaataga acattctctt gaaaccttac ttcgtgagct tctacttttg
    33541 tttccttata ttaaagccga aacggacaat atttcgtttc tgccttttct tcatcagata
    33601 gtttttgcaa cttcatatac tacacttatt ttctataaaa gtcaacactt cctggcgttt
    33661 cttctgcttt ttcgcaacat tttcttaatg ttttcaattt tatgttttca attatctgac
    33721 aattcttaat tgacagacct ttttatctcg gttatacttt agaaaattca ttttgtggag
    33781 gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat aacttttact
    33841 ttagctactt tagataggtt tgtattcatc atgatggata caaacgttaa acgacagttt
    33901 gtatctatga tggctaaaga actttgtatt gcagcaaaaa gccacgtaga caaaatgcta
    33961 agtggctgag cagtgaaaat acaaagtaaa agtatcaagc attctacttt ttcaatttat
    34021 tttccgactt ttcaatccat ttggacattt tgtctgaatg gattttttgt ttcccaaaat
    34081 taaggaggaa aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg ccctgctact
    34141 agctttagga ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg gacaaaaaac
    34201 aattggcgta ttgcaacttg tacaacataa ctcgttagat gctgcctata aaggattcaa
    34261 agaaggatta gcagaagaag ggtataaaga aggcgataat ctaacaatcg attatcaaaa
    34321 tgcccaaaac aatcaagaca acctgaaaag tatgagtgag aaactcgtca aagaagatcc
    34381 tgatttgcta cttggaatcg ccactccagc agcacaatct ttattaaatg aaacaaccac
    34441 tatcccgatg actgtcacag cggtcacaga cttggaacaa gcccatttag tcacatccaa
    34501 tgaaaaacct ggcggcaatg tgactggcac gacagatatg gtgccaatcg aaaaacaaat
    34561 cggcttactg ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt ataacaacgg
    34621 tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa aagcgggcgt
    34681 caaagtcaaa gtactgacag caaattccac aaatgatgtc caacaagtga cgaaaagtct
    34741 agctaaagat gtagatggaa tctatattcc tactgataac acgttcgcaa gtgcggcaag
    34801 tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg cagttgatca
    34861 agtcaaagac ggcggtctag caacagtagg aatcgactac gaaagcttgg gcaaacaaac
    34921 agggatcatg gcagcaaaaa tcttagacgg caagaatcca agtgaacttc ctgtcgaaac
    34981 agcgaatgat ttggaactgt atgtcaataa agacatggcc aaagcattag gcatcaatcc
    35041 agacagcatc aaagaagctg aataaaacac aggaggattc tcatgacaaa tttgctctct
    35101 cttttcttat catctgcatc gcaaggggtc ttatgggccc tacttgcgat tggtgtatac
    35161 gtcactttcc gtatcttgga cattgccgac ttgactgctg aaggaagctt tcctttaggt
    35221 gcagggatcg cggcagtaag tattacaaat ggctatcatc cattgacagc ttgtcttcta
    35281 ggctttttag ggagtgcggc agcagggctt gtttccggac tgcttcacac aaaattgaaa
    35341 attcccgccc ttcttgcagg gatcatcacc atgactggtt tatattctgt cacatcccgt
    35401 gtgatgggtg cgccgaacat ctctttattg ggtcagccaa cgatctttca ttgggtcgag
    35461 tcattcggca taacaaaaga caacgctgta ctgatcgtcg gtctgctttt tgctttgatc
    35521 gttgtatgtt tacttgtttt gttcttacgt acagaaacag gacttgctat tcgtgcaact
    35581 ggcgataatc tggcaatgag cgaagcaaac gggatcaaca cagataatat gaagatcatc
    35641 ggttatatga tttctaatgg atgcattgct ctttctggct ccttgcttgc gcaaaacaac
    35701 ggctttgctg atttaaattc tggtattgga acgatcgtaa ttggacttgc ttctattatc
    35761 atcgcagaag ttttgttacg taaccagcca ctatttctcc gtctcttgac tgtcgtttta
    35821 ggtgcggtca tttatcgttt tattttagcg ttagtcttcg aactgaatgt tgaaccaagt
    35881 gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc gcagatccag
    35941 caaaaattcc atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat gacgtcttaa
    36001 cattggaaaa tctccatcat acctttgaaa aaggaacgat caacgaaaat catgtcctga
    36061 aaggcattaa tctttcctta gatcccggcg acttcgtgac gatcatcggt ggaaatgggg
    36121 ctggtaaatc tactttgctg aacagcatcg ccggtacatt ttctgtcgat cagggtcaaa
    36181 ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg aaatccatca
    36241 gtcgcgtatt ccaagatcct aaactaggga cagccgttcg tttaacggta gaagaaaatc
    36301 ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc aaaccacaag
    36361 accgaacatt tttcaaagac cagctggttc ggctaaattt ggggttggaa aatcgtttat
    36421 cctcagaaat cggtttgctg tctggaggac aacgtcaagc tattacgtta ttgatggcca
    36481 cattacagcg tccagaactg atcttattag atgaacacac tgcagcgctt gaccctgcta
    36541 cttccaaaac ggtgatggaa ttgacagaaa agctgatcac tgagcagaat ctcactgctt
    36601 ttatggtcac tcatgacatg gaagacgcga tccgctatgg gagtcgactg atcatgctgc
    36661 atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc acagtgcccc
    36721 aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat caagtacttt
    36781 tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc ttcttttttc
    36841 tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa aactattttg
    36901 ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg acaacgaaag
    36961 acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa aagttggcac
    37021 agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg gaaaaggcag
    37081 gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac gtactagatg
    37141 caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta caaacgatgt
    37201 tgacttctga atctacgatg aaagatgcca aactggcagc agctcaagga gattctgttt
    37261 cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga cggccttttt
    37321 tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac aaacggtttg
    37381 atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc attgaatctg
    37441 taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat ggaaaaaaag
    37501 tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc agtcatatca
    37561 tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct gatctccaag
    37621 aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat cagctaatca
    37681 gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg ttggacttct
    37741 ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa ggaacgactt
    37801 atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa acaaatgaac
    37861 agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg caataattat
    37921 cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa ttctcttcaa
    37981 aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa ggtattctaa
    38041 aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa acaagagagt
    38101 catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc ttatagatag
    38161 accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt aagttctttt
    38221 ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca cctgaccaga
    38281 gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga ctggtggtgt
    38341 ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta agtaagaaat
    38401 agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat tagcttggat
    38461 ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta tcgtcatcgg
    38521 aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta aatcagcaat
    38581 ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt tgatctgcgg
    38641 ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt caaacaaacg
    38701 ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat aatccattta
    38761 ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac ctacttatat
    38821 gttactacac acccttattt tttacacttt aacagtcacc atcatcaata gtttggagat
    38881 tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata tgaacgtttc
    38941 attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac taggagaaaa
    39001 agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa aaagggcaag
    39061 tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg ccgtttcagc
    39121 ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg gtaactatgg
    39181 cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa ctgtgaatgc
    39241 acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt ttggtacaga
    39301 gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg attctttcgc
    39361 taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg ttggcagtaa
    39421 cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc ttgatcactt
    39481 aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt tcgagcagga
    39541 atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag ctaaaacata
    39601 tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg atttatcaga
    39661 taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata cgccacttca
    39721 aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt cttctgcatt
    39781 gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag aagatttttc
    39841 ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa tcagtgctcc
    39901 ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa accaagacgg
    39961 aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg atcctaacga
    40021 aatatttatc agcgacaatg gtacagatac gacagacaca agcgacagta cagatacatc
    40081 taccacaagt gatagctcag attcatcaac ttcaagcgac agtacagata ctacctctac
    40141 cacaagtgat agcacggata catcagcttc aagcgacagt acagatacta cctctaccac
    40201 aagtgatagc acggacacat cagcttcaag tgacagtaca gatactacct ctaccacaag
    40261 tgacagcacg gacacgtcag cttcaagcga cagtacagat actacctcta ccacaagtga
    40321 tagcacggac acatcagctt caagtgacag tacagatact acctctacca caagtgatag
    40381 cacggacacg tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac
    40441 ggacacgtca gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga
    40501 ttcatctacc actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac
    40561 atcctctacc acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc
    40621 gtctaccaca agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa
    40681 caggacgaac tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag
    40741 agcacttcct aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt
    40801 acttgtaatc ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa
    40861 tattcaagat aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta
    40921 tcataaatac aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa
    40981 atttatttac gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta
    41041 agggaaaaga gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc
    41101 aaagcctttt ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt
    41161 tcaacgattt tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa
    41221 taaggtagta gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc
    41281 ccttgacgca tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct
    41341 aaaatatcat cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt
    41401 tttgcgaagc gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac
    41461 agttcagctg tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta
    41521 tagcgatcat ccatctgacc taattcgcca ttcaatactt tttccatact gcgagaattc
    41581 aattggagac ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat
    41641 aaatagtctc ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga
    41701 ccacgtcgca gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc
    41761 atttcgatag aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt
    41821 gagaacaata gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg
    41881 attgcttgtt cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt
    41941 tctgtcaatt ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa
    42001 aaactccttg tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc
    42061 ctgctcataa tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc
    42121 tgcgatcccg attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa
    42181 catgactgtc catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat
    42241 gaaaccgccg gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac
    42301 caataacatt ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat
    42361 cgttccgcct agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtctcg
    42421 ttttttcatg gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc
    42481 aggcgcaaaa gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg
    42541 tgcaacaagt agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct
    42601 aataaatcag actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat
    42661 gtggcaaaca gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct
    42721 ggtcatcgca atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat
    42781 aatctccgtg ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga
    42841 atgttttgat ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt
    42901 tttgctgaag accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa
    42961 taaaaacatc ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg
    43021 ttctcctttt tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc
    43081 tgtgtcttgc tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat
    43141 tctttgggct tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga
    43201 cagaagtggg caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt
    43261 gtgaaatttc ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg
    43321 ttgtaacaga ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat
    43381 tcctttaatt ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa
    43441 atcttgcatt caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc
    43501 taccgtacca ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc
    43561 caatcgcacg tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta
    43621 cccagtaaaa catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc
    43681 atagccatgt gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca
    43741 tgaatttttg gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg
    43801 tgaaccagtc tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca
    43861 aaccaaaatg tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag
    43921 ctgtggttgc atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc
    43981 atgtccaagg gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg
    44041 gtttgaacac ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac
    44101 catagaaaat acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc
    44161 gagtcacgtt gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat
    44221 aataaccaga acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag
    44281 ctaaaaatcc gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa
    44341 tagaaaccat aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg
    44401 aagccacgat attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg
    44461 gtgtcggtgt tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt
    44521 tatcttcgta gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg
    44581 attctaatcc aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg
    44641 tgtgagtcgg aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga
    44701 ttggtgcatt caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag
    44761 catcatttcg atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga
    44821 tttcgtttgg atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc
    44881 caatcatttc aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac
    44941 gcagttcatc atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa
    45001 tccttacaga atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac
    45061 caaagtcatt cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta
    45121 atttggtctt gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt
    45181 gtacattctt ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta
    45241 cacgtccgcc agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga
    45301 tcagagtaat tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa
    45361 caccggcata tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc
    45421 ttatagacta tctatataat gaatatatga taattctttt agtttgatac ttattacaca
    45481 atctccatat attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca
    45541 aattttaaaa taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac
    45601 atgggttatt tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa
    45661 tcacaaagaa aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac
    45721 gttttgtaac tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg
    45781 cagatgacaa cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg
    45841 caaaaagcac aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca
    45901 catataaatt agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg
    45961 taaaagacgg caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa
    46021 aaacaaaaga tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa
    46081 aatacattcc tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag
    46141 tcgtaactgg agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc
    46201 aagctgctca agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag
    46261 acggcactta caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa
    46321 tgacagttgc tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta
    46381 aatcaaaaca agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac
    46441 caaaaactta catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg
    46501 gctcacctgc taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca
    46561 tgtacgcaca acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag
    46621 ataatatcgt aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac
    46681 gatagccaga ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa
    46741 aaaactacta ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag
    46801 caactcagca aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc
    46861 atattctgat gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg
    46921 caaagaatca gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac
    46981 gatcaatcaa aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt
    47041 aaaagtttct gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc
    47101 tcaaggcgga ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga
    47161 tgacgccaga aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca
    47221 taagatcgaa ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat
    47281 cgatttaggt gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa
    47341 acaaggagtg accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag
    47401 cccaagaggc gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg
    47461 atctgtttta ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga
    47521 gcgttattta gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta
    47581 tccgttcgac aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga
    47641 cgggttgtct acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag
    47701 cgaattgaat aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac
    47761 cgatccaatc aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg
    47821 ttctgaacta aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga
    47881 aatacgaaca aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc
    47941 tttttttcat gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt
    48001 tgatttagcg actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt
    48061 atataagtat gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa
    48121 catgatttta gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac
    48181 gggttggctt ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt
    48241 cggaccgatt cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg
    48301 ccttggaatt tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct
    48361 gacagtcaac ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc
    48421 aatcgtatta gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa
    48481 tttgcgggat cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg
    48541 acgtcctgcg ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt
    48601 aatggggttg ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc
    48661 aattgtgaag aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag
    48721 ctattccatc aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac
    48781 aatcattatg caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa
    48841 gtaataagaa cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta
    48901 ccgcagatgc aagcttttga acaccgttta tca
//
LOCUS       DAZUMO010000015        47469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000015 DAZUMO010000000
VERSION     DAZUMO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.015"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG8ER_000828"
     CDS             complement(54..788)
                     /locus_tag="ACG8ER_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5049428.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG8ER_000829"
     CDS             1115..1525
                     /locus_tag="ACG8ER_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS5049429.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG8ER_000830"
     CDS             1518..2219
                     /locus_tag="ACG8ER_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS5049430.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG8ER_000831"
     CDS             2219..3805
                     /locus_tag="ACG8ER_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049431.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG8ER_000832"
     CDS             3824..4045
                     /locus_tag="ACG8ER_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS5049432.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG8ER_000833"
     CDS             4042..4803
                     /locus_tag="ACG8ER_000833"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS5049433.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG8ER_000834"
     CDS             5183..5692
                     /locus_tag="ACG8ER_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5049434.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG8ER_000835"
     CDS             complement(5767..6306)
                     /locus_tag="ACG8ER_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS5049435.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8ER_000836"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8ER_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS5049436.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG8ER_000837"
     CDS             7319..8170
                     /locus_tag="ACG8ER_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS5049437.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG8ER_000838"
     CDS             8198..8833
                     /locus_tag="ACG8ER_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS5049438.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG8ER_000839"
     CDS             8853..9626
                     /locus_tag="ACG8ER_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5049439.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG8ER_000840"
     CDS             10034..10831
                     /locus_tag="ACG8ER_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS5049440.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG8ER_000841"
     CDS             10809..11222
                     /locus_tag="ACG8ER_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5049441.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8ER_000842"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8ER_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5049442.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8ER_000843"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8ER_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS5049443.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG8ER_000844"
     CDS             15999..16559
                     /locus_tag="ACG8ER_000844"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS5049444.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8ER_000845"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8ER_000845"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS5049445.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG8ER_000846"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG8ER_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS5049446.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8ER_000847"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8ER_000847"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS5049447.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8ER_000848"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8ER_000848"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS5049448.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8ER_000849"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8ER_000849"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS5049449.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG8ER_000850"
     CDS             21906..22484
                     /locus_tag="ACG8ER_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5049450.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG8ER_000851"
     CDS             22536..23018
                     /locus_tag="ACG8ER_000851"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS5049451.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG8ER_000852"
     CDS             23135..23509
                     /locus_tag="ACG8ER_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049452.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24187..25539)
                     /gene="brnQ"
                     /locus_tag="ACG8ER_000853"
     CDS             complement(24187..25539)
                     /gene="brnQ"
                     /locus_tag="ACG8ER_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5049453.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25686..26276
                     /locus_tag="ACG8ER_000854"
     CDS             25686..26276
                     /locus_tag="ACG8ER_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5049454.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26404..27753)
                     /locus_tag="ACG8ER_000855"
     CDS             complement(26404..27753)
                     /locus_tag="ACG8ER_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5049455.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27921..28661)
                     /locus_tag="ACG8ER_000856"
     CDS             complement(27921..28661)
                     /locus_tag="ACG8ER_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5049456.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28674..30266)
                     /locus_tag="ACG8ER_000857"
     CDS             complement(28674..30266)
                     /locus_tag="ACG8ER_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5049457.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     regulatory      complement(30404..30578)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(30834..31130)
                     /locus_tag="ACG8ER_000858"
     CDS             complement(30834..31130)
                     /locus_tag="ACG8ER_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049458.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31306..32031
                     /locus_tag="ACG8ER_000859"
     CDS             31306..32031
                     /locus_tag="ACG8ER_000859"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS5049459.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32024..32866
                     /locus_tag="ACG8ER_000860"
     CDS             32024..32866
                     /locus_tag="ACG8ER_000860"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS5049460.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32869..33390
                     /locus_tag="ACG8ER_000861"
     CDS             32869..33390
                     /locus_tag="ACG8ER_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS5049461.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33643..34206)
                     /gene="def"
                     /locus_tag="ACG8ER_000862"
     CDS             complement(33643..34206)
                     /gene="def"
                     /locus_tag="ACG8ER_000862"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5049462.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34457..34726
                     /gene="rpsO"
                     /locus_tag="ACG8ER_000863"
     CDS             34457..34726
                     /gene="rpsO"
                     /locus_tag="ACG8ER_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS5049463.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34914..37040
                     /gene="pnp"
                     /locus_tag="ACG8ER_000864"
     CDS             34914..37040
                     /gene="pnp"
                     /locus_tag="ACG8ER_000864"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS5049464.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37550..37816
                     /locus_tag="ACG8ER_000865"
     CDS             37550..37816
                     /locus_tag="ACG8ER_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5049465.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37960..39951
                     /locus_tag="ACG8ER_000866"
     CDS             37960..39951
                     /locus_tag="ACG8ER_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS5049466.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40305..41606
                     /locus_tag="ACG8ER_000867"
     CDS             40305..41606
                     /locus_tag="ACG8ER_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5049467.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41736..43106)
                     /locus_tag="ACG8ER_000868"
     CDS             complement(41736..43106)
                     /locus_tag="ACG8ER_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS5049468.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43288..43611)
                     /locus_tag="ACG8ER_000869"
     CDS             complement(43288..43611)
                     /locus_tag="ACG8ER_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5049469.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43936..44544)
                     /locus_tag="ACG8ER_000870"
     CDS             complement(43936..44544)
                     /locus_tag="ACG8ER_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS5049470.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44874..45590
                     /locus_tag="ACG8ER_000871"
     CDS             44874..45590
                     /locus_tag="ACG8ER_000871"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS5049471.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45603..46298
                     /locus_tag="ACG8ER_000872"
     CDS             45603..46298
                     /locus_tag="ACG8ER_000872"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS5049472.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46382..47011
                     /gene="udk"
                     /locus_tag="ACG8ER_000873"
     CDS             46382..47011
                     /gene="udk"
                     /locus_tag="ACG8ER_000873"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS5049473.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15390 a         8788 c         9878 g        13413 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcacagcat gacagcattg
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc actagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tttgcgaaaa ctatttttat atcatatata tgtctaaaat ctatattata
    23881 aaagaaatgt tgtttgatca gcttctcttc ggtattagtt caaaacaaaa cggcaaagta
    23941 tatggagcta ctttgccgtt ttgtttctct aatactcagg tcagtttata gacgctcaat
    24001 aaacggtgtt caaaagccag tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa
    24061 gccattttca aaaaatattt cttaatactc gaaactgagc ggctttttca cgacctctca
    24121 ataaacagtg ttcaagagac agcctatcgg caataaggct gaacgtctct ttcacaacct
    24181 cttttatcaa tctgctactt cttctttctt agcgaaactc caaatcagtc cgataacaaa
    24241 accgataact gctggtacga tccaccccat ccccaatttg aaaaacggca agtagctttc
    24301 cgcaacatat aaaagatttt gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt
    24361 caacccatca aaaatcgaag caatcaatgt aaaataagta gtcattcgat aaacagccgt
    24421 acgctgctta aacaaaggac caactaacac tagtaaaatc agtgtcattg ctaatggata
    24481 gatgaacatc aaaacaggca acgaaaattg aatgatattc gtcaagccta cgtttgccgc
    24541 taaacaagga aggatactag caagtgcaac aaagaatgcg taatttcttt gcggaaacaa
    24601 atctgtgaat gtttctgcaa aagctgtaat caaaccaata ccggtcttca agcaagctaa
    24661 tataacgata aatgctaata agatactgcc ataagtacct aaataatgat cagcaatctg
    24721 agccagcgtg atcccgccat tttcacttag agcaaagcta cctaagctca tagcacctaa
    24781 ataagcgagc aaagtataaa tgattcccat caatataatg ctgatcgcac cagatttgat
    24841 cgtgtcttta gcgatttcag caggttttgt tacacccatg ttttggattg ttgtgacaat
    24901 gatgatccca aatgctaaag cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc
    24961 tgtgaaaaaa gcatgctctt gataatttgg ctgaataggc gcttgattta cagaacctag
    25021 tggattcaaa aaagcaagga gaagcaaagc acctaataaa accaaaaatg caggattcag
    25081 gaactttcca acataatcta atatctttgt cggttttctt gaaaaccacc aagccagcac
    25141 aaaaaataca atactgaaaa ttgctagata tagcgtctgt ctgctaggat cgataaatgg
    25201 tgctaaacca atctcgaaag atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat
    25261 aactaagtaa agcaagatgg taaaaaccat cgcatatgtt tgatttatcc gttttgctaa
    25321 ttcatataca cctgagcttt tcgatacacc aatggcaatc acccctaaga agggaagacc
    25381 gataccggta accaagaaac ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc
    25441 caaatgaacg ggaaagataa gatttccagc accaaaaaat agaccgaaaa gcatggagcc
    25501 tataaaaaga tagtctttaa atgataattt ttttgtcata cataactcct ttacggtttt
    25561 tttaaaagta acaaaatttc gaaaaatatg caacaatttt cagaaaattt cctatataaa
    25621 acgacggtta tttaataacg taagtgataa aataaaagcg ctgaatcatc tgaggaggaa
    25681 tcatcgtgac aaagtcgaga ttagaagcat ttactgatgg agtggtagcc attgttttga
    25741 gagttcttgt attagagatc aagatcccta atacatctag ctttcattct ttatgggtca
    25801 tccgaaatac attattagct tataccatca gctttatatt tgttgcggtg atctgggtga
    25861 accatcatcg tatatttcag atggcagaac gaattaatta ccgagtggtc tggtcgaata
    25921 tctttttgct cttttggctg actttatgtc cttctgttac tagttgggtc gggcgtaacc
    25981 ctgaagcttt ttggcctgag atggcttatg tcttagtata cacgatgtgg agtttttctt
    26041 ttggtatctt gatgaggcaa atcatgaaag caaataaacc agattcccat gtcgtgagag
    26101 ttttgtcaaa ggaccgtcga agtcttatct ccatgatgat taacttatgc ttgattgccg
    26161 gtgtcttttt tgtaccgttg atcgggttat tcggacgttt ttttgtatct ggtatttgga
    26221 ttttttccta taaaaaagca gatgcctatt atcagcggtt gtttccgggg aaatagaaaa
    26281 gaggctggga cagaagcttt taactccaag aaataagccg aaatttcaca aaaatttaag
    26341 aagcaatttt cgtaaattcc ttcttatttc tcgaagctga ccacttctgt cacaactact
    26401 ttcttacttc ttacgttcat atttcatatg atagattagt aatagaagca agaaccacac
    26461 aggtgttagt aataaagcga ttctcgtatc ttttcctagg aacaagacga cacacacaaa
    26521 ggccatgtaa agaaatacca gataattact aaatgggtac aacggcattt taaatgaatg
    26581 agcttttttg ccgaggatac ttttacgata tttcatgtgc gtgtagacaa gcatactcca
    26641 gataaacaag aaacaagtag tggcaatact gcttactagt gtgaatactt cactaggcat
    26701 cacgtaattc aagataacag aaatgaaaat gacgatagta gaaaatgtca aagcattccc
    26761 tggcacatga tgtgttgtta actttttgaa ttttttaggg gcactgcctt cttgcgctaa
    26821 agaacgaagc atacgtcctg tactatatat tgcactgttg caggcagaag ctgctgcact
    26881 taatacgact aaattgatca aggaagcagc aatcgtgatc ccaatttccg aaaatacttc
    26941 tacaaatgga ctgttatctg gattcaagct gttccatgga tagattgcca taatgacaaa
    27001 aagagatcct aagtaaaata agatgatcct gataggaata ttattgatag cttccggcaa
    27061 cactttttca ggattttctg tttctccagc gactaatcca actaattcta tcccagcaaa
    27121 agcaaaggtt accatttgga aagctaagat aaatcctttt gcaccagttg gaaagaatcc
    27181 gccatgagac catagattcg tgatactggc tgtgccaata tctgtcgtat agtgggtgag
    27241 gatcatataa gctcctacga ggataaaggc aatgatcgct acaactttga tcaatgcaaa
    27301 ccaaaactct aactcaccaa acaatgagac agccaccata ttcaagcaaa gaagaagacc
    27361 taatgcaatc agttcaggaa gccactgagg aacaccaggc gcccagtagc gtacatacat
    27421 tccgatggct gttaaatcgg ccattgcaat cgatacccag cagaaccaat acgtccagcc
    27481 agtgatgaaa gccgcttttt tgccaagata ttctgcaaca aaatccagaa aagaatggtt
    27541 attggtgttt gataacagaa gttcgcctaa cgcacgcata atgaaaaaac agatgcatcc
    27601 agtaataaga taggcgagca atatcgaagg gccagctagc tggatagact ttccggaacc
    27661 taaaaacaac cccgttccaa tcgctccgcc aatagaaatc agttggacat gtctattttt
    27721 cagtccacgc tgcagttctt gctcttcatt catttttaaa tcatcctaac taaaaaagtt
    27781 taattacttc catattaaaa gagttttctg aaaaacacaa ctaagattcc aaaattttca
    27841 gaaatttacc ttttatgagt aattcataaa aaagaactgc ggtaaacacc gcagctcttt
    27901 gtcccaatca gctattgatc ctaagcattt aaaatacgtg aaaggaattc ttttgtacgt
    27961 tcttctttcg gatggacaaa gatatcttct ggtgtacctt cttctgcaat cacaccttta
    28021 tccataaaaa tgacacggtc agatacatct tttgcaaatt ccatctcatg cgtcacgata
    28081 agcattgtca agccagtatg tgctaagtct ttgatcgtat taagcacttc gccaaccatt
    28141 tcagggtcta acgccgaagt aggttcgtcg aatagcaata catctggatt cattgataat
    28201 gcccgagcaa ttgcgacacg ctgtttctgt ccaccagata attgtgctgg tttggcttcg
    28261 atataacgat ccataccaac tttttccaga ttttcgattg ctactttttt ggcagtttct
    28321 ttatctcgtt tcaggacagt tgtttgacca gacatacagt tttccaagac attcatattg
    28381 ttgaacaggt tgaaggattg gaaaaccata cctagattcg ttcgatattt aggtaaatta
    28441 tatttaggag ctaatacatt ttctccatta taaatgatct cgccgcctgt tggtttttcc
    28501 agtaaattga tacagcgcaa gaaagtagat ttaccagaac cggaagaacc gatgatcgtc
    28561 acaacttcac ctttattgac tgttacgctg atatctttca atacttcatt ttcaccgaag
    28621 ctttttctta gatgtttgat ttcaataatt ttttcattca tgattcttcc tccttattcc
    28681 tgcaaatttg aattgtcgat ttcttcaagc ttggtataag ctgaaggacc atcgattttc
    28741 ttttctaccg cccgcaggat tctggtgatc gtaaaggtca tgactaagta gatgatcccg
    28801 acgatcgtaa atgtttggaa gaattggaaa ttcgcaccgg aagcagagtt tccttggaag
    28861 aacaaatcag ctacgccgat tacacttaaa actgctgtat ctttgatatt gataacaaac
    28921 tcatttcctg ttgctggcaa gatattccgt aaaacttgtg gaataacaac ttttctcatc
    28981 gtttggccat gggtcatacc aatggcttgc gcagcttcga actgcccttc atctactgca
    29041 aaaatccccc cacgaacgat ctctgtcatg taagctccag tattgagaga gacaataaac
    29101 aaagcggcaa tcgttcggtt caatgaaatg ccaaaagcga gtgccagacc gtagaagatg
    29161 accattgctt gtaccatcat cggtgttccg cggaatactt cgacatagat agaaagaagt
    29221 gcatttccga ttttttggaa gaagcgagtc agtgcatttc ctgattcagg taccgaacgg
    29281 aagacaccaa tcagtaatcc aagtgtagta ccgacaactg tcccgatcaa tgcgatgaac
    29341 aaggtaagtc cagcacctcg taagaacata ttgccgtatt gtttccaaat cgtagtgaaa
    29401 tcagaaatga tgccaggttt ttcttcacta tcagttgcag caggctgatc tttgactgct
    29461 tggtccataa ttttggttcg ttcatcgtta gagatgccag aaagaatctg gtttactttt
    29521 tggatatccg ggtcgccttt acgcatacca acagaaatct gaacatcttc cgggtttgtc
    29581 tggaagccat ctgcaccgct gaattcaagc atttccaagt ctttgttcac actagttgcc
    29641 gttacacctt ccggacgttc gctgacataa ccatcgatca ttccagaagc taaagctgtt
    29701 ctcattgctg agaaattatc cattgcttgt tgtttctgca catctggtat ctgatcgatc
    29761 acagtatagt ggaatgtatt taactgagca gtaatctttg ctccagaaag atctgctaaa
    29821 ctttttgctt ttgcatattt gctatctttt tgaactacta ctactaattg tgattcgtaa
    29881 taaggatcgg taaaatcaat ttcttttcgt cgttcagcag tagggctcat tccggcgatg
    29941 atcgcgtcga tcttgcctga ttgaagggca ggagccagac catcccattt cgtttggacg
    30001 atgactaatt ttttccctaa accatctgct acttttttag cgatttgaac atcataaccg
    30061 ccggcgtaac tattttggcc ttgaattggc aaggcattat tctcatctgt ttgttgagac
    30121 cagttgaagg gtgcataccc agcctccata cctacgcgaa actcatcgct cggtgttttt
    30181 tcatctgcat ttgccagaga tcccacactg aatagtgaga agacaaaagc aaatagtacg
    30241 gcaactgaaa ccatattttt tttcattttt ttctcccttt ctgattatat gatacacgag
    30301 cggtctagat gaaatgcggt aaagctttat ctgaaaaaat aaaaagcgac cgatttatag
    30361 atataaaaag gccgctaaaa ttccataagg tgataaacct ctcacaaaac atagcgcaac
    30421 ttagcatcac tgctaagaca gtccgcaagc tcttaacttg cgtcccaaca tacactccaa
    30481 gcaaaaaagt gcacatttcg gcgatcctcc ctattcctgc ttctccatgt ttgcttcact
    30541 ccacagggtt aatgattctc gcgacctcta cctcattgat tgaggcatct cgtattcagt
    30601 tatttataca aaaaaagttt acgatactcg agaggctttg tcaatctata actgctgttt
    30661 tttgaaaaaa tactgataaa tatagaaatc tttactttta ccgaaaaaac aatcaaaatg
    30721 tgaatcatct atccattgca aacgatagta taattataca caaaaatgta tgtaatacaa
    30781 taaaaaaaga actagaacag aaaatttcct gtgtccagtt ccatctcgat catttaagct
    30841 gccgctttct ttggctcacg tcctaggatc gcttgcaaaa gaacaacgac gccataaatg
    30901 gctaatatta gataaatcaa acttgagtat tgggtgattc cccagcccca tgttcgatat
    30961 acggctaatg taatggctaa aacaattgcg gcaatcacgt tgaataaagc aaaagcccaa
    31021 agatttccat tttctttgcg tacaagataa aaaacagaga aataaacgct taacgctaac
    31081 caggcgatac aagccagaat aacgccccaa taaataaaga atggtaacat atcgattcac
    31141 ctcactttta tacagcagta ttgtaacacg ttttcattct tttgtcagtg aacattgtga
    31201 taaattgttc aaatgcccat aacacttttt tattcccatt tttctgactt ttcaacaata
    31261 aatttgttcg agtatgggct ataatggaaa gtgaggtgac aaaacatgaa acaattaaca
    31321 gtagacgaag cagcaaaaga gatattagct tatcagcaaa ttacattttt gaccggtgca
    31381 ggggtatcca caccttctgg agtgccagac tatcgatctt tgtcaggaat ctaccacggg
    31441 atggagcagc cggaatactt gctgagtcat caagcaatgg tgaatgaacc ggataaattc
    31501 tatcaatttg tcaaaaagat ttatcatcct gaagcaaaac ccaatatcat tcatttggaa
    31561 atggcacatc tagcgaaaga aaaaaatgtt tgggttgttt cccaaaacat cgacggtttg
    31621 cacgcaaagg ctaaaagccc gcaactcgtc aattttcatg gaagtttgta tcaatgtcat
    31681 tgtcgaaaat gtcaacaaac ggtagactgg aaagaatatc ttcacagcga caaacatcaa
    31741 gcatgcggag gacaaatacg cccagacatc gtgttatatg gtgaagggtt tcaagatgga
    31801 gtcatggagc aggcggctta tgccgtcagc caagcagagc tgatcgtcat tgttggaacg
    31861 agttttcaag ttcatccgtt ttgtgatctg gtacagtttc gccagccgtc agccaaaatc
    31921 ttagtaatca atcaaacgcc tgtttattta gcggagcctt atacgtttat tcaagaagac
    31981 ggagcggttg ttttcaaaaa aatacaagaa ttaggagcag attatgactg aattcaaaac
    32041 cgaaaaagaa aaaatgattg caggcgagct ttattttgcc aatgatcctg agctggttgc
    32101 cgaccgtatg tttgcaagaa gtcaaagcca aatcatcaac caagcagaat cagcggaact
    32161 gcgatcccaa ttgttgaaag agacttttgg ccgtacaggg aaaaaaatct atatggagcc
    32221 agttatcaat tttgattacg gatataatat atttgttggg gagaattttt atgcaaattt
    32281 caattgtaca tttttagatg tcagcacgat tgaaatcggt gataactgca tgttcgctcc
    32341 gaatgtccag ctttacacgg cgacgcatcc actacatcca gtgaaacgta acagcggtct
    32401 ggagtacgca aaacccatta aaatcggtaa taatgtttgg ttaggcggag gagttatcgt
    32461 cacgccgggt gtcactctag gggacaacgt agtagtcggc gcaggttcgg ttgtgaccaa
    32521 atcttttccc gacaatgtag tgattgcagg aaatcctgct cgtatcatca agacagtaga
    32581 ggaagaacta acagaagagt ctcttgaaac attacgtcat aagatcgata tgatcgatcg
    32641 agagttgatt gctttattag aaaaaagaat ggatacggta actaaaatag gacaagtgaa
    32701 aaaagaagaa aaccaagcag tttatgatgc aaaaagagag cagcaagtat tggataaagt
    32761 tgttagtctg ctgaaaaata agggatataa ggaaacaata acagatacgt atgttgattt
    32821 gatgaaacac tcgcgcgaat accaaaacaa aatgaaggag gaataagtgt gatcgaagga
    32881 gagacacggc gaaaacaaat catgcaaacc ttggcaaata cagaaaaacc agtgagtgct
    32941 agcaagtttg cgaaaacttt tggagtcagc cgtcagatca ttgtaggcga tattgcttta
    33001 ctacgagctg caggtaaaag tatcgtggca acagcaagag gttatatcct cgaaacagaa
    33061 gaaaccaata ctgggcacat cagtaaaatc gctgtccagc atggaaaaga tcaaacagaa
    33121 gaagaactga gactaatcgt tgaaaatggt ggtgaaatca ttgatgtaat tgtggaacat
    33181 cccctttacg gtgagttagc aggaactttg cacatcaaaa cgccacaaga tatcgattcc
    33241 tttatgagac gttatcaaaa aagtaatgct actttgttat ccgaattaac aggcggcatc
    33301 catctgcata cgatccgcta cccagaaaag catatgctga aaaacatcaa acaaaaacta
    33361 gctgaagcag gaatcctcta cgaaggataa catcgtagct gctacttttt atcctagaaa
    33421 ctattctatc atctctgaga taaacaccca tatggaagaa ataagaaaaa ataagaaaag
    33481 atgcgtatcc aatacacaga attttttgga attgcgattt ctagatgtct atctaaattt
    33541 cgattagtgg agaaacataa aaaagggaag tgcagctttt gagcactccc cttttttata
    33601 aagctttctt gtagcatagt atcgaaggaa cctgccaatt ctctactcga tcaccaatac
    33661 gccttctttt agtgcaaatg gattttgatc gttgatgtgg tcatagaaca tcacaccgtt
    33721 gatatggtcg atttcatgtt gaacgacgat cgattcataa ttcttcaaac gaatcttgtg
    33781 tttttcacca ttcatatcat aataagacac agtgattttg gcatggcgaa caacatatcc
    33841 tggtacttct cgatcaacag ataaacagcc ttcaccttcc cctaaacaag catcttgaac
    33901 agaatggctc aaaattttag gattatacat aacagtactt agagaaggtc catctgcctc
    33961 tgggtcagga cttggtacgt gaacagcaat gattctttta gaaatatcta attgaggagc
    34021 agctaagcca acaccgcctc gtaagtgtag ttcttcagct ttgattggat cttggctgtt
    34081 ttttaagaac tcaagcatct cttttcctaa agagatatct tcttcgctta atggaagaga
    34141 aacttctttt gcaacttcgc gtaaggtcgg atttccttca cgaatgatat catccattgt
    34201 aatcatggaa tatgtacccc tttcttaaaa gactgtgttt caagtctatt ttcgcattat
    34261 agtttaccac aaaaatggaa aagtagtagt aagattgttt gaaaactacc ttttcttaat
    34321 gattaactac ttgattttca acccggtttc aggtaaagta tagctgtgtg ctctaggcac
    34381 atgacctgcc acttggttaa gcgaatcacc aacgttttac tcagtcgcag gggaataatc
    34441 gaaagaggtg aaagaaatgg caatttcaaa agaacgcaag aatgaaatca tcaacgaata
    34501 cgctcgccat gaaggggata ctggttctcc agaagtacaa atcgctgtat taactgaaga
    34561 aatcaaccac ttgaatgaac atgcacgcgt gcacaaaaaa gaccatcatt cataccgcgg
    34621 cctaatgaaa aaagttggtc accgccgtaa cttgttagct tacttacgta aaactgacgt
    34681 tcaacgttac cgcgaattga tcaaacgttt aggcttgcgt cgttaatttt agtcaaaaag
    34741 agtgggattc acatgaatct cacttttttt actcgtatat caattaagta aaattccgtg
    34801 atcaagctag atggactcct actaaccaaa tgaaagctgc tcaaaagacg gctttcggca
    34861 gttttcattt gtttagtagc gtcttgcttt cacgagaagg aaaaggagaa gttatgtcag
    34921 aaaaacaagt attcaaaacg acatggggcg gccgtccctt acaaatcgaa gtcggtcagc
    34981 tagcaaaaca agcaaacgga gccgttttag tccgttatgg agatacagta gtcttaagtg
    35041 cagctgtggc ctctaaagaa gcaaaagata cagacttttt cccattaacg atcaattatg
    35101 aagaaaaaat gtatgcagca gggaaaatcc ctggaggttt catcaaacga gaaggccgtc
    35161 caagtacgga agcaacattg actgcccgtt tgatcgaccg tccaattcgc ccgatgtttg
    35221 cagaaggctt ccgtaatgaa gtccaagtaa cgaacatcgt gatgagtgta gaaacagact
    35281 gctcgccagc tatggcagca atgctaggtt cttctttagc actatcaatc tcagatatcc
    35341 catttgacgg cccaatcgca ggcgtagaag ttggtcgtgt aaatggcgag tacgtcttga
    35401 atccaacagt agaacaagca gaacaaacag atatcgaatt gactgttgcc ggaacgaaac
    35461 aagccatcaa tatggttgaa agcggcgcga aagaagtttc agaagaagac atgcttggtg
    35521 cattgctgtt tggttttgac gctatcaaag agttagttgc tttccaagaa gaaatcgttc
    35581 aagcagtagg gaaagaaaaa atggaagtga ctttactgca agttgatgaa gtattgaaaa
    35641 aagaaatctt tgatgcttct tacgctacaa tgaaagcagc agtaatgact gaagaaaaac
    35701 ttgctcgcga agataatatc gagcaggtga agatcgatat ccgcgaagct tatgctgaga
    35761 aatttgcagg tcatgaagat gaagatcact tgttgaaaga agttaaacaa atcacagaag
    35821 atcttgaaaa agatgtcgta cgtgaattga tcaccatcga taagatccgt cctgacgggc
    35881 gtaaattgga tgaaatccgt ccattgtcat cagaggtcag cttattgcca cgtgtacatg
    35941 gatcaggttt gttcacacga ggacaaacac aagctttatc agcttgtaca ctcgcacctc
    36001 taggagagca ccaaatcatt gatggtttag gggtggaagt cagcaaacga ttcatccacc
    36061 actacaattt ccctcaattt tctgttggtt ctacaggacg tgcaggctct ccagggcgtc
    36121 gggaaatcgg acatggcgca ttaggtgaac gtgcgttagc acaagtaatc ccttcagaag
    36181 aagaatttcc ttatacgatc cgtttagtag cagaagtatt ggaatcaaat ggttcttctt
    36241 cccaagcaag tatctgtgct ggcacattag cattgatgga tgcaggtgta ccaatcaaag
    36301 cgccagtcgc cggtattgcg atgggacttg tttctgatgg tgaaaactat acgatcctaa
    36361 ctgatatcca agggctggaa gatcatcttg gcgatatgga ctttaaagtt gccggtacaa
    36421 aagatggtat cactgcttta cagatggata tcaagattca aggaatcaca gaacaaatcc
    36481 taactgaagc attaactcaa gcaaaacaag cacgtatgga aatcttagaa gaattgactt
    36541 caacgattgc tgctccacgt gaagaattaa gccaatatgc accaaaaatc gaaatgatcc
    36601 agatcgaacc tgctaaaatc aaagatgtta tcggtaaagg cggcgatacg atcaacggaa
    36661 tcatcgatga aactggtgtg aaaatcgata tcgatcaaga tggaaaagta agtattgctt
    36721 cagctgatgc agaaatgatc aaaaaagcta tcaagatcat cgaagatttg accaaagaag
    36781 tcaaagttgg tgaagtttat ctagggaaag ttgtccgtat cgaaaaattc ggtgcattcg
    36841 tcaacttgat caaaggaaaa gatggacttg tccatatttc ccaattagca aacgatcgtg
    36901 tcaataaagt cgaagatgtt gttaaactag gtgatgagat ccttgtgaaa gtaactgaaa
    36961 tcgataaaca aggacgcgta aatctgtcaa gaaaagcaat gttgaacgaa gatggttcaa
    37021 cgaaaaatga agaaaaataa tttaattcat aggaataatg tacttgtgat cgtgaaggtg
    37081 acagggggac aataattatt cgaaaaaagg ggactgtgac aaaagccgtg tcacagtcct
    37141 tttttttcga ataaacggtg ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt
    37201 aaaaacatta ggagctgttt ttatttggct gttcttatta cttgatgcag aacagctttt
    37261 ttacaacctc tccaataaac gatgttcaat cagctgtccc tcgatattag ctcaaaaaac
    37321 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggtc aaaccgctga
    37381 tttcacaacc tctgattcac ggtgttccag aagcagcttc tacggaaata agctgaacac
    37441 ttttgttaca acttcttttt ttctggagtt tcttggcgca tatttttgga gtccttctga
    37501 ataaagcgta aaataaaagt gagaaatatg gataaaggag ggaaaggaga tgctgaattt
    37561 tttatggtcg ttatttatcg gcggtatatt aggtttgata gctggcgcaa ttttaggaaa
    37621 ggaagtcccg ggaggcgtat ctggaaatat cattatcggt tttttaggta gttggctcgg
    37681 cgaattttta ctaggaccac tgggaccgga actcggcggt ttttatttga taccagcatt
    37741 attgggtgca attttttgcc ttgctcttta ttcatttgtg gcaagacgta tcagagaacg
    37801 tccaaagaaa gaataatttt attcaaataa gttgacactt cagtgtgtta cttggataat
    37861 tagaagcaat ttaggaaagc gggtgtcatg gataagaata tcatctatca ttcaagctta
    37921 cctaaaggat atccatataa caagaaatga gggaaaaacg tggcaacaac gcacaatacc
    37981 aaaaagatat caatgactgg cctgcttgta gcgatgggag tcgtctacgg agacatcggg
    38041 acaagtccgc tttacgttat gaaatcgatt gttgaagata atggcggatt gtcaggaatc
    38101 aatcctgatt ttgttatcgg atcagtctca ttgatttttt ggacattgac tctactgaca
    38161 acaatcaaat atgtcgtgat tgcattgaat gcagataacc atggtgaagg tggaattttc
    38221 tcactctaca cacttgtccg aaaaggtggg aaatatttga tcattcctgc aatgatcgga
    38281 ggagctgcct tactagcaga tggggtattg acccctgctg tgacggtaac gactgcgatt
    38341 gaaggcttgc gaggaattcc gcagtttttt gaccggttcg gtaataacca gaacgtgatc
    38401 gtcattatca ctttagttat tatttttgtt ttattcatgg tccaacgctt tggtacagat
    38461 attgtcggaa aagcattcgg accaatcatg ttcgcttggt ttactttttt aggcgtaatg
    38521 ggattaatga acttcagtca agactggaca gttatccgtg cactgaatcc ttattatgct
    38581 atccagctgc tttttagtgc agataacaaa ttaggcttgt tcattcttgg aaatattttc
    38641 ctggctacga caggtgcaga ggcgctttat tccgatcttg gacatgtcgg caaacgaaat
    38701 attcgtctga gctggcctta cgtcaaaatt tgtttggtta tcaattattt tgggcaggct
    38761 gcgtggttgt tgaatgttta tcaaaaccct gaagcacagc aaatcaagaa cctgaatcca
    38821 ttcttccaaa tgatgcctca aagttggatc gtgatcggcg ttgcttttgc aacagtcgct
    38881 gcagtcattg cttcgcaagc attgattaca ggttcgttta cgctagtttc agaagcaatc
    38941 aagttgaaac tgctgcctcg gttaaagatc atgtatcctg ggaacagtat cgggcaaatg
    39001 tatatcccag cagtcaatct gattttatgg atcgcctgtt ctctagtggt tgtcacattc
    39061 cgaacgtctc atcatatgga agctgcttac ggtctatcga tcactgtgac gatgttgatg
    39121 acaacagcac tgctttactt ttatcttatc caaaatgggc attctcgttg gcttgcatat
    39181 ttagttacct ttttcttcgg tgcgatagaa gtcgttttct ttatctcaag tgttgtcaaa
    39241 ttcttccgag gcggttttgt agccgttctg attggtttga tcatcttagt cgtgatgttt
    39301 atttgggaac aagggaacat tattcgcgaa tcgtcaaccg aggatgtgtc tttgagagat
    39361 tatatcccgc agcttagaga attacgtgat gatacttctt taccaatcta tcagacaaat
    39421 gtggtcttct tggttcctga tatggctgaa gataaagttg gccgacagtt catctattcg
    39481 attttagata aacggcctaa aagagcacga gtctattggt ttgttcatgt ggaagtgacg
    39541 gatgagccat atacgaaaga atatcaagta gatatgatgg acactgattt tattgtccaa
    39601 gtgaatctgt tcttaggttt cagggtccaa caggaaatca acgtatatgt tcgccaagtc
    39661 atccatgatt tgatgaaaca aggccgtttg ccaaaacagc cacaaagata ctctctgaca
    39721 ccagggcgag aagttggcga tttccaattt gtcctgattg atgaagtagt ttccaatgtg
    39781 acaactctag gaaaatggca acgtcaaatc atgcaggcaa aactggcaat caaaaaaatc
    39841 gctacctcgc cagaaacatg gtttgggcta gagtacagcg aagtaaaaca tgaaactgtt
    39901 ccgcttatca ttggaaatac tcggaaaaca tggttgaaag agcggttgtg aaagtagcgc
    39961 tttgacctga actaatgtcg aatgtcagct acttgaacac cgtttatcag aggtcatgaa
    40021 aaagctgtac tgcaacaagc agtaagaacg agcagataaa aacagcttct catgtttttc
    40081 atctgtttgg gcttaatgcc gtagatgcaa gcttttgaat accgttttta tcagaggtca
    40141 tgaaaaagct gtttagcaac aagtaacgca atgattttga agtgataagg agatgtggct
    40201 gaaaaatgcc gcatctcctt ttttattcct aaatatgaat acgcattatt ttcaatcttt
    40261 ttgtagaaaa agggtacaat agaaaaagag aaaggcggga gaatatggtt atcaatttca
    40321 ttattttatt tttgatgatt gcaattactt cattttttgt agcaagtgaa tttgctttag
    40381 tgaaaatcag acgatcaaga ttggaacaac tagaaaagga aaatgtaaag aacgccaagc
    40441 tagctcttca tgttacacac catttagata actatttatc agctagtcaa ttaggaatta
    40501 cgctaaccgg tttgatcatc gggtgggtcg gtgaaggatc tgtcgcggct ttactagaac
    40561 cagtgatcgg taaactacct tttagttcgg ctattagcag tacaatctct gtggcgctgg
    40621 gatttatttt agttacgtat atcgatgtgg ttattggcga actgcttcct aagagctata
    40681 gtatcgtcaa tacggagaag gtcgtcttgt tcgtagttaa accacttcat tatttttata
    40741 aagtaatgtt tccttttatt tgggtattga atcattcagc tgcagggcta gggaagctat
    40801 taggtgtgcg tctcgtatct gaaggagaag agacattgtc tcaagaagag ctgacattag
    40861 tcgcattgaa ttcttatgaa aaaggagagt tgaccaaaga agaatattat tatttggaaa
    40921 acgtgtttga atttgatgat accttggcaa aagatataca agtagaccgt acgtccatgc
    40981 aagtattcga agcttcagaa accgtcaaag aagcgataaa agcttcgatt gaacaaggac
    41041 atactcgcta tccggttatc ttagaatcaa aagacaatgt tttaggttat gtcaccttgc
    41101 cagatttgat caagcaatct tataaagatg accagctgac agtagaacag ttgattgaag
    41161 agccgatcgt aacaactgaa acgatcccta taaaaaaatt attgacgatc atgcgaaaaa
    41221 agggtaagca tattgctatc ttaaaagatg aatacggagg gacaagcggc ttagtgacga
    41281 ttgaagatat tttagaagaa atcgtcgggg aaatccgaga tgagacagat ttggacgaag
    41341 cattgatagc ggaacagtca gatggctctt atatcatctc cggcaaacta acattagatg
    41401 atttccagcg ttattttcat gtagagattc cagagtttga agaaacgaat tttactacac
    41461 ttgcgggctt tgcttctagc cgatataaag aaataaaagc aggaacaatc attgagattg
    41521 catcattccg tttcacggta ttagaatacc agcatgcaca tatcgattat ttcaaagtag
    41581 agtccacgga aagaaaaaca gaataaaaga aaaaaggctg tgacattttt tgtcacagcc
    41641 tttttgttat ctaaaaaacc tgttttcggt aaacgaagaa gatattttca ataaaagttt
    41701 tcatttttag ccgcaacgat ttcttttaat caaggctatc ttgcttctgc atgttctaat
    41761 gtgaaatgtt cctcttcacc catgtgtttc aattgtttca tcgaacgcat ataagagtaa
    41821 agcaacacgg caacagaacc gatccaagct aaaggagaag ctgctgctgc tcctgggtat
    41881 ccccacattc ctgtaagaac gatagctgca aaagaacgca taagcaattc catcataccg
    41941 gcgatcgtag ggatcttgct ttgtccaagc ccttgaagtg tgtagcgtaa aatgaacagg
    42001 actgctaaga tccagtaaag agacccattg atattgaagt aggtttgtgc taattcaaat
    42061 acacgtgttt ctgatgggtt aacaaacaat tgaacaaaga aatgaccgaa gaaaatgacg
    42121 atagcacctg ctaagagact aaatccgaca ctcatcagta aagtttgacg taccccttgc
    42181 aagattcggc catattcttt cgcaccatag ttttgtgcag tgaaagttgc cattgcaata
    42241 ccaaatgaca tcatcggctg atttgcaaat tggtcgatac gaccagcagc ggcttgagca
    42301 gctacgacat ctgtccctag actatttagt gctgcttgta acacgatagc accaacagcg
    42361 atgatcgaag attgaaacgc cattggcaaa gcagcattta ggtgtacagc aaactcttct
    42421 ttttcaaatt tgaaatcttt tttgcgtaac tgtaacaaag gcattttttt cttgatatac
    42481 actagacaca gtacgctgga agaaacttgt gcgattactg tagcaatagc tgcaccttcc
    42541 acacccatgc caaaatagat aataaagatc aaatcaagta cgacgttgat gatgaccgca
    42601 atgatcagaa agaatagtgg tgtgcggctg tcaccaagtg cgcgaatgac attagaaagc
    42661 aaattgaaac tgactgatgc aaagatccct aataaaataa ttgaaataaa tgtttgtgct
    42721 tgatcaagta tttcaggagg agtttgcatc aattgaagca tcggacgaat aaacaggaga
    42781 cttaggacag ttaaaatcac agtgacgaca agactaatga caacactagc agcaaaactt
    42841 ttcttcagac cccgataatc ttttgcaccg taacgttgtg cggtaataat ggctaaacca
    42901 gctgttaaac cttgggcaaa gccaatgatc aaaaatgtca ggcttccagt tgcaccaacc
    42961 gctgccagcg catttttacc tagcgtctga cccacgatga tcatatcgat catattgtag
    43021 aactgctgaa aaacatttcc gatcagtaaa ggaacagtga acatcaaaat cagtttagcg
    43081 gggcttcctt ttgttaaatc gcgcatcttt taaactcctt atatctttaa aatttagtta
    43141 gtagtatata gcgtttatcc tgagaatacc aattagaaat caatgataat ttatgaaact
    43201 ccataaaaag aagccaagaa ttgagtcaaa gaatcaacaa cttggcctgg tctgtaaaat
    43261 aagggacatc cacgtttatc tatcacctta atattgtcta atctcacttg gtaaaaggat
    43321 cgtttctctc gtaccgtcat cataggcgta tacttgagca ggaaattggt tcgaataggc
    43381 aaacggcaaa tcaatatcca tttccacatt gcttcctgct tgagaaaaat gcatcgtcac
    43441 ttttccacga ttttcgatca aatcaaaggt taatatattt tctaatggaa tgactccctt
    43501 aagatctagg tcaataataa accatatact gtcaatcaat tcatctggca aacttgatac
    43561 gacaccaact gaagcgtaac ggttgcggct gctatcaaaa ctttcaaaca tctgtttaac
    43621 ctccttcgaa tcaacgaatg ttttattttt ataattaatc ctaggataaa ctagaaatac
    43681 ttacttcaac gagggttcat tctaataaag aatcaggaaa aatgcaacaa taattgaata
    43741 ctttttttca aagtataaat ttttataaag gtacgataaa agtatgcgag gaaaagcttg
    43801 gtaaattagg atttttcgtt tgtaaagcca tttacaaaag tggaagcccc atatgaaaat
    43861 aaacgattag aaataaaaaa ggcgtgttaa aagctgctga cccgccgctt ctaacaccac
    43921 ctgattgttt cagtattatt cttctgttga accgatcact tctggttcgc caccttcacc
    43981 tgtgctttct tcttcttcac tttgagcgac tacagcagcg atttgctctt cactgtcggt
    44041 cacgaatttg taatcgtcgt tttttggaag atcggctact gtaatagaat cacctaattc
    44101 caagtttgtc acgtcaactt caattcgttc aggcaatttg tctggtgtag cagatacgac
    44161 tactgtatac aagttttgag ctaacactcc accagattta gcacccactg attcgccaac
    44221 aagaacgact tctgtttcga cttctgtttc ttccgacatg tttacagata gaaattctgc
    44281 atgttcgaaa tgattggtaa aagtgttcgt ttggatcttg tgaaccaatg tattgacttt
    44341 ttgtccatcg atgtccatcg tgatgactgt gttcgctcca tgttcacgca acaatttgct
    44401 tagtgtcaaa ccatcgaatg agataggggt actttctact ttatacccat aaacaacagc
    44461 tggaactttc ccttcgtgac gaagtttgtt tcttaatgaa cggggacgta cttctctttt
    44521 acttacttct aatgatactg acataaccaa aattcctcct taaaatgtga ctgtctgctt
    44581 tggtaacgga cagattttat ttaagtgaat tctaaaggct aatctcgtgc acatcatcga
    44641 aggcagggcg cttaaaaaag cgcaaaagga gagatgcgcg catctcccct tagaaagtca
    44701 ggattcaact tttctccacc aggtcattac gctgtgtatg agaaatccaa ttcactttac
    44761 tatttaaccg taacgtgaac tgttaattaa gtcaaggaat aactatgatg atgtatttgc
    44821 aagcaagaat attcaaggta taattaatta gtagtaataa gaaaggaaac agcatgcgtt
    44881 tagataaatt aatagaagaa aaattagaaa cgacacgcaa ggaaatgaag cggctgtttg
    44941 ctttgaagca agtgatgata gatggcacag tcgaatttcg acaaaatcga aatgtagata
    45001 gtttactgca tcaaatcgaa gtagcaggtg aacacttaga aacaaatgag tcctattatc
    45061 ttctcaataa acctgaaaaa gctgttacag ctgtatcaga tccagaaaag acgacagtaa
    45121 tcgatctgat tgcaagtgcc gatcgtttcc aaccattata tccagtggga agactagata
    45181 gagatacaac aggactattg ctgatcactt cgaatggaca attgggatat gaaatgctac
    45241 ttccagaaaa aaaggtttca aagacttatc aagtagttgt gaacgaaagg gtgacaccgg
    45301 cagacgtaac agcatttgaa aatggaatcg tattcgctga tgggtatcaa tgtaagccgg
    45361 ctgatttaaa aatactcaaa gcagacaagc accaaagtac ggttttattg acgatccaag
    45421 aaggaaaatt ccatcaagtc aaaaagatgt tcttagccca aggaaagaaa gtgaccgcat
    45481 tgaaacggct aagcatggga ccattggaat tagatgaaca gctaggagta ggcgagtatc
    45541 gatcgttgac attagcagag atgaaaaagt taagtattta ttttaaatag gaggtcaaaa
    45601 agatgaaata taaaacttta ttgtttgatg tagatgatac attgttagat tttcaattaa
    45661 ctgaagccaa tgctttaagt gctttgtttg cagaacaagg aatcacattg acaccagaaa
    45721 tcgaaaccag ctataaggct ttgaatcatc atttatggca ggagtttgaa aaaggcagac
    45781 tatcacgaga agaaataata ggcagtcgtt ttgggttact tttccagcaa tttgggaaaa
    45841 cagtagatag tgatcaaatg gaaaaacgct accgccacta cttgaaccaa ggtcatgatc
    45901 ttgttccagg aagtctagaa atcctgaaaa aggtccatcc gcatgctgat ctttatattg
    45961 tgacgaacgg tgtgtcgcac acgcaacatc ggcgattaac agatgcagga atgcttgatt
    46021 acttcaagga tatctttgtc tcagaagctg tcggagcgca aaagccgatg aaagaatttt
    46081 ttgatcatgt cttttcccac attcctaatt ttgataaaaa gcaaactgtg atcattggtg
    46141 attctttgac ttctgatatc aaaggaggaa atatggcagg gatcgatacg atttggttca
    46201 atagaaataa attaccagaa atcgaagaaa tccagcctac ttatcgaatc gattcattag
    46261 aggaacttta ttcattattg gaaattgaaa aggattaatt ttcagaaaaa tatagcattt
    46321 tcgtgttttt tcgctataat agattagaat gaaaaaacgg atacgaaaga ggttttagtc
    46381 aatgacaaaa agcaagccaa ttatcattgg tgtgacgggt ggatccggaa gcggaaagac
    46441 aagtgtcagc cgtgcgattt tcaacaactt cccggaccat tccatcatga tgcttgaaca
    46501 agattcttat tacaaggatc agagccattt gagttttgaa gaacgattga acacaaatta
    46561 cgatcaccca tttgcctttg ataatgatct gctgattcag catgtaggcg acttactgaa
    46621 ctataaagcc attgaaaaac ccgtgtatga ctatgtagcg catacaagaa gtcaggcaac
    46681 gatcattcag gaaccaaaag aagtgatcat tctagaagga atcctgattt tagaagacga
    46741 acgtctgcgt gacttgatgg atatcaaagt atacgtagat acagatgatg acattcgtat
    46801 cattcgtcgt atcaaacggg atatggaaga acggggacgc acattggact ccgtgatcga
    46861 acaatatttg acagttgtta aaccgatgta tcatcaattt atcgaaccaa cgaagcgcta
    46921 tgcggatatc attgttccag aaggcggaga aaaccacgtt gctattgatt tgattacaac
    46981 aaaagtagct agtttcttaa accataaata gtgcgcagcg aaacagttga acggactgcg
    47041 accgtttgaa aagcaaggat tctgcttttt gaacagtcac cagccaactg tttttttatt
    47101 tataacaaga ggttgtgaaa acagcatctt gacctaagta ttaaaaagaa gaaacggaaa
    47161 atagctgttt atattttacc gttttttgag ctaataccga aggtcagctg attgagcacc
    47221 gtgaagtaga ggttgtaaaa aagctgtctt tgcatcaagc gataagagca tccaaataaa
    47281 acagctcctc acgtttttcg ttgggttggg cttattatcg tagatgcaag cttttgaaca
    47341 ccgtttaacg agaggttgta acagaagtgg tcagatgcaa taaataagaa gaaacttacg
    47401 aaaattattt ctcgaatttg tgtgagattt cgacttattt ttttgaagaa gctcttttgt
    47461 cgcaccccc
//
LOCUS       DAZUMO010000016        46145 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000016 DAZUMO010000000
VERSION     DAZUMO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.016"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(448..978)
                     /locus_tag="ACG8ER_000874"
     CDS             complement(448..978)
                     /locus_tag="ACG8ER_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS5049474.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            1083..1634
                     /locus_tag="ACG8ER_000875"
     CDS             1083..1634
                     /locus_tag="ACG8ER_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5049475.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1787..2260)
                     /locus_tag="ACG8ER_000876"
     CDS             complement(1787..2260)
                     /locus_tag="ACG8ER_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5049476.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2398..3870
                     /locus_tag="ACG8ER_000877"
     CDS             2398..3870
                     /locus_tag="ACG8ER_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5049477.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3867..4577
                     /locus_tag="ACG8ER_000878"
     CDS             3867..4577
                     /locus_tag="ACG8ER_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS5049478.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            4929..5213
                     /locus_tag="ACG8ER_000879"
     CDS             4929..5213
                     /locus_tag="ACG8ER_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS5049479.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(5494..5913)
                     /locus_tag="ACG8ER_000880"
     CDS             complement(5494..5913)
                     /locus_tag="ACG8ER_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS5049480.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(5915..6499)
                     /locus_tag="ACG8ER_000881"
     CDS             complement(5915..6499)
                     /locus_tag="ACG8ER_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS5049481.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(6546..8021)
                     /locus_tag="ACG8ER_000882"
     CDS             complement(6546..8021)
                     /locus_tag="ACG8ER_000882"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS5049482.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8202..8513
                     /locus_tag="ACG8ER_000883"
     CDS             8202..8513
                     /locus_tag="ACG8ER_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049483.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(8628..10964)
                     /locus_tag="ACG8ER_000884"
     CDS             complement(8628..10964)
                     /locus_tag="ACG8ER_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS5049484.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            11106..11774
                     /locus_tag="ACG8ER_000885"
     CDS             11106..11774
                     /locus_tag="ACG8ER_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5049485.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11777..12820
                     /locus_tag="ACG8ER_000886"
     CDS             11777..12820
                     /locus_tag="ACG8ER_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5049486.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13057..13938)
                     /locus_tag="ACG8ER_000887"
     CDS             complement(13057..13938)
                     /locus_tag="ACG8ER_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5049487.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            14097..14825
                     /locus_tag="ACG8ER_000888"
     CDS             14097..14825
                     /locus_tag="ACG8ER_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS5049488.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            14981..15298
                     /locus_tag="ACG8ER_000889"
     CDS             14981..15298
                     /locus_tag="ACG8ER_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5049489.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            15291..15899
                     /locus_tag="ACG8ER_000890"
     CDS             15291..15899
                     /locus_tag="ACG8ER_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS5049490.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            15954..16745
                     /locus_tag="ACG8ER_000891"
     CDS             15954..16745
                     /locus_tag="ACG8ER_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS5049491.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            16883..17371
                     /locus_tag="ACG8ER_000892"
     CDS             16883..17371
                     /locus_tag="ACG8ER_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5049492.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            17422..18294
                     /locus_tag="ACG8ER_000893"
     CDS             17422..18294
                     /locus_tag="ACG8ER_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5049493.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            18470..18832
                     /locus_tag="ACG8ER_000894"
     CDS             18470..18832
                     /locus_tag="ACG8ER_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5049494.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            18841..19593
                     /locus_tag="ACG8ER_000895"
     CDS             18841..19593
                     /locus_tag="ACG8ER_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049495.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            19607..21409
                     /locus_tag="ACG8ER_000896"
     CDS             19607..21409
                     /locus_tag="ACG8ER_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049496.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            21686..22726
                     /locus_tag="ACG8ER_000897"
     CDS             21686..22726
                     /locus_tag="ACG8ER_000897"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5049497.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(22778..24922)
                     /locus_tag="ACG8ER_000898"
     CDS             complement(22778..24922)
                     /locus_tag="ACG8ER_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5049498.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(25279..26652)
                     /locus_tag="ACG8ER_000899"
     CDS             complement(25279..26652)
                     /locus_tag="ACG8ER_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5049499.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSSKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(26652..27047)
                     /gene="rbsD"
                     /locus_tag="ACG8ER_000900"
     CDS             complement(26652..27047)
                     /gene="rbsD"
                     /locus_tag="ACG8ER_000900"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS5049500.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(27075..27992)
                     /gene="rbsK"
                     /locus_tag="ACG8ER_000901"
     CDS             complement(27075..27992)
                     /gene="rbsK"
                     /locus_tag="ACG8ER_000901"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS5049501.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(28100..29113)
                     /locus_tag="ACG8ER_000902"
     CDS             complement(28100..29113)
                     /locus_tag="ACG8ER_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS5049502.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(29194..29793)
                     /locus_tag="ACG8ER_000903"
     CDS             complement(29194..29793)
                     /locus_tag="ACG8ER_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5049503.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            29979..30680
                     /locus_tag="ACG8ER_000904"
     CDS             29979..30680
                     /locus_tag="ACG8ER_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049504.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            30690..33401
                     /locus_tag="ACG8ER_000905"
     CDS             30690..33401
                     /locus_tag="ACG8ER_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5049505.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            33852..35480
                     /gene="treC"
                     /locus_tag="ACG8ER_000906"
     CDS             33852..35480
                     /gene="treC"
                     /locus_tag="ACG8ER_000906"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS5049506.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(35540..36721)
                     /locus_tag="ACG8ER_000907"
     CDS             complement(35540..36721)
                     /locus_tag="ACG8ER_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5049507.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            36973..38496
                     /locus_tag="ACG8ER_000908"
     CDS             36973..38496
                     /locus_tag="ACG8ER_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5049508.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(38565..39293)
                     /locus_tag="ACG8ER_000909"
     CDS             complement(38565..39293)
                     /locus_tag="ACG8ER_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5049509.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(39306..40586)
                     /locus_tag="ACG8ER_000910"
     CDS             complement(39306..40586)
                     /locus_tag="ACG8ER_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5049510.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVALIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(40727..41329)
                     /locus_tag="ACG8ER_000911"
     CDS             complement(40727..41329)
                     /locus_tag="ACG8ER_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5049511.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            41574..41780
                     /locus_tag="ACG8ER_000912"
     CDS             41574..41780
                     /locus_tag="ACG8ER_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049512.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            42092..42811
                     /locus_tag="ACG8ER_000913"
     CDS             42092..42811
                     /locus_tag="ACG8ER_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5049513.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(42892..43575)
                     /locus_tag="ACG8ER_000914"
     CDS             complement(42892..43575)
                     /locus_tag="ACG8ER_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS5049514.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            43793..45979
                     /locus_tag="ACG8ER_000915"
     CDS             43793..45979
                     /locus_tag="ACG8ER_000915"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS5049515.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        14917 a         8199 c         9223 g        13806 t
ORIGIN      
        1 tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt
       61 tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
      121 ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga
      181 tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag
      241 cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct
      301 gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa
      361 aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc
      421 tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta
      481 ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc
      541 attgtctata cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt
      601 tggataagtt tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa
      661 agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag
      721 tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt
      781 gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg
      841 gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca
      901 ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa
      961 ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac
     1021 atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac
     1081 tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga
     1141 tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa
     1201 aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc
     1261 aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga
     1321 aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg
     1381 aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta
     1441 ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa
     1501 cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc
     1561 atgcgatcaa tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta
     1621 accgaaaaaa ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa
     1681 acgcagatca ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt
     1741 agcatctact tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta
     1801 acaactgttc gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg
     1861 tctcgttgtc atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta
     1921 aaatcgtata gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac
     1981 ggacatggtg cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa
     2041 atccgtcatc taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt
     2101 ttctaggaca attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc
     2161 ttaattgacg tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt
     2221 gatcattttc caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc
     2281 tcttgcttcc cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg
     2341 aatttaggac taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg
     2401 agtaacaacc aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt
     2461 gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt
     2521 cctacattga tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt
     2581 ttcttgctag tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt
     2641 tcaagaaatt tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt
     2701 acagcaccta attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga
     2761 gtatcgatgc cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga
     2821 tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta
     2881 tctggttata tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc
     2941 gttgttcttg tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat
     3001 cctagcattg atattttatc cgctatttta tcaacgattg gattcggtag tttactttac
     3061 ggattttcta gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt
     3121 gttggtgtga ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc
     3181 ttggaacttc gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta
     3241 acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt
     3301 ggagaatcag cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct
     3361 atgatgcctg tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca
     3421 gggatgttca ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca
     3481 gttttatata tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg
     3541 ccggtaacga cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg
     3601 gtaaataata cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg
     3661 ttgacgaatg tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta
     3721 gcttataaag atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta
     3781 gcgatcatct ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct
     3841 gttctaatga aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg
     3901 cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt
     3961 tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg
     4021 gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag
     4081 gagcagatat gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc
     4141 gaacaaatga aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag
     4201 tagaaaagac agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa
     4261 acgacatgta taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag
     4321 tgaatacatt caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt
     4381 tagctgaatt agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc
     4441 aagacggtat ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac
     4501 aacgtacgaa agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag
     4561 aagcaaaagg cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc
     4621 atcaagcagt aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct
     4681 tagtgccgta gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt
     4741 taactctgag tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt
     4801 gacttgtgac cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca
     4861 agacttttgt cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga
     4921 gaaataaaat gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca
     4981 agggcggggc atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag
     5041 ttaaagtcca agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg
     5101 tgaaaaatcc agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa
     5161 acatcggaaa agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt
     5221 tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt
     5281 agtaagtcaa atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact
     5341 cgaaacgaag cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg
     5401 gtattagctc aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact
     5461 caggtcataa cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata
     5521 ataaactggt tctttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc
     5581 taactcttct gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg
     5641 gataccggaa gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt
     5701 ttcggctaag acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg
     5761 catctctgta tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt
     5821 taaagtgatc aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag
     5881 tttggttact tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat
     5941 gctcatttac aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt
     6001 ggatcgtttg aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc
     6061 gtgctaattt agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa
     6121 tgactaactt tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc
     6181 ctaaaccaac agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat
     6241 cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat
     6301 caaacaggct ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg
     6361 cccatggact gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta
     6421 ggttgatggc ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc
     6481 gttgtgccaa ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc
     6541 attcattatt tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa
     6601 tgttctttca tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg
     6661 aaacgaatag agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc
     6721 attcctggga tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact
     6781 acatcgttca tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct
     6841 ttgaaggctg ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt
     6901 tctttacgga tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca
     6961 tttactactt ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga
     7021 ataccggcca tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg
     7081 tcttttcggt gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca
     7141 gtatagccag gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt
     7201 gtttcatttg gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca
     7261 tcgactgcta taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat
     7321 agttcattgt agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct
     7381 ggatctaaac caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt
     7441 tggaagtgac caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct
     7501 tctagaacca tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag
     7561 actacaccca ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa
     7621 atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg
     7681 tctgctttgt ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga
     7741 ccaagtgttc gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt
     7801 ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct
     7861 tcttgagtcg gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat
     7921 tctgcttcag gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt
     7981 tcttcgatga cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt
     8041 gtctttacct atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa
     8101 agtattctac aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta
     8161 tcactaaaag aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt
     8221 catttcctaa agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa
     8281 acggagaagt aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat
     8341 ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga
     8401 ttgggactga agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt
     8461 atacgccaga agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa
     8521 ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac
     8581 tatttcagcg ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc
     8641 aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc
     8701 gtcaatatag tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca
     8761 ttttttagat ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca
     8821 ataaacacac ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg
     8881 cgagtgatgt cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt
     8941 tcaatcacag atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc
     9001 cctgcaaaag caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc
     9061 atcgttccag ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa
     9121 tcatctagat aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata
     9181 cttgaaggca agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca
     9241 actttttcag tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat
     9301 tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat
     9361 aagagattca ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca
     9421 gaagcattct gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt
     9481 tttgtatagt aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa
     9541 tcaagttttt cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta
     9601 ttattattgc cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca
     9661 agtaaagaat aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa
     9721 cttgacactc ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta
     9781 tcaggatttt gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga
     9841 ttgattcctg tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt
     9901 acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg
     9961 acttttgcag taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca
    10021 tcgtcacgag tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt
    10081 tcagcttgcg tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg
    10141 attccgatga tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt
    10201 cggatattgt tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag
    10261 ttaaattgat catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca
    10321 tcagaaaatt caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc
    10381 atgatcacta atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt
    10441 tctgtatcta acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt
    10501 gctcgagcga tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc
    10561 atatgatttg tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct
    10621 ttagaaacac cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca
    10681 atcaagttgt aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca
    10741 ctgtccttaa agtctttagt agactttccg ttgattacca tatcaccgga atcataatta
    10801 tctagtccac caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc
    10861 gctacgaatt ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt
    10921 tcgccgactt tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg
    10981 tattgaaata ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata
    11041 aatgttaact gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga
    11101 gatcaatgaa tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt
    11161 ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga
    11221 aaaaactaga agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg
    11281 atggctatga cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga
    11341 ttacggcaaa ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt
    11401 atatgacaaa acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac
    11461 gttcaaagat caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg
    11521 cttatacagt gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc
    11581 tgtataaatt actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata
    11641 tttggggatt agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa
    11701 ggacccgttt tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata
    11761 aggcggttat ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag
    11821 tattgtcttc atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt
    11881 gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt
    11941 cgtctttgcg gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg
    12001 tatcctgcat ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt
    12061 ttcgattcga atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt
    12121 caacgttatg gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag
    12181 tgtatcccat gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca
    12241 agccccaaat ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat
    12301 cacgcagctt tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg
    12361 tatccaactg gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt
    12421 ttttcagcct aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa
    12481 ttacacaggc aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat
    12541 aaagtacaat caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat
    12601 tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt
    12661 tgaaaaattt tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc
    12721 actggttaaa aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg
    12781 agtaggaacg acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta
    12841 aaaattctat tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt
    12901 cttacggttc ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga
    12961 ggtgtcattg aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct
    13021 ttttttgggt ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata
    13081 ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata
    13141 actctctgtt gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac
    13201 tgttttttcc ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc
    13261 gattcttgct cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag
    13321 tacaggttga tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc
    13381 gcttagatcc aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa
    13441 atcgtctgtt tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc
    13501 aaagtggatc gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac
    13561 ttctttcaac tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa
    13621 acctttataa ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt
    13681 cgtataggtt tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata
    13741 tgggagcaat aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa
    13801 tttgacgtct aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt
    13861 gaattgtcga tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc
    13921 gaatttatgg atatccatca tattcctcct acttattcca ataaagaata ctttaacaca
    13981 aaaaatgaat tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata
    14041 attacagaaa tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc
    14101 caactggagt tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag
    14161 taggaaataa attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt
    14221 cgatggggat gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg
    14281 ctttagtgat aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg
    14341 gggctgcgct gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga
    14401 cccacgatga atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta
    14461 cggggatcta tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta
    14521 aatctgtcct tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt
    14581 ctgtgatagc aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta
    14641 tttatccatt gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga
    14701 tggtcgctac cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac
    14761 ctgcaatgat cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt
    14821 tataataaaa atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt
    14881 cagttttttt gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga
    14941 ttgaaataaa atattacact tgagaataga aggtgacgta atgcacatcc agattccaac
    15001 tgttcttctt gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc
    15061 cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt
    15121 gagaagattg aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa
    15181 ccgtcgatat tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga
    15241 atggattgtt tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat
    15301 atttgaatga agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga
    15361 tccagtatta tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg
    15421 aatatggtac accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag
    15481 aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa
    15541 tcattatcgg tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg
    15601 ttattggggg actattggtt gctttactag cgctcatttt tagtatctat gttttatgta
    15661 tcagtatctt aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat
    15721 cgattctgag tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat
    15781 ttatttcgcc actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg
    15841 ttaaatggat gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa
    15901 ggaggacaca taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta
    15961 ctggtggcct cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg
    16021 ataatggtcc tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca
    16081 aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga
    16141 taaaaacgac ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga
    16201 ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac
    16261 aaatcactat tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata
    16321 atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg
    16381 gctgggtatc gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct
    16441 cacttcgatt agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact
    16501 tccatctaga agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt
    16561 tatctttgca tgagatggaa aacagtggct atactatcca aacgtctggt gaatcttata
    16621 ttgaaaaaga ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa
    16681 agatcactgc taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag
    16741 attgatcctg tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt
    16801 gctgtttttc atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa
    16861 agaaaacagg gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac
    16921 agaaatggaa agattacata cttatatcac tcaactaagc caaaccttct atgatccaga
    16981 caaagaaaaa gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt
    17041 gtaccatgaa gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt
    17101 accgattatc gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa
    17161 atctgttcgg gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga
    17221 ctattttaca gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc
    17281 cggagggaca caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg
    17341 tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga
    17401 taaaggagcg caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca
    17461 gggattgtcg gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg
    17521 tggcaagcga aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg
    17581 ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga
    17641 caatatttag gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa
    17701 atgactgttt cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg
    17761 gatggaaacc ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag
    17821 gcattattcg atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt
    17881 cggcatatcg ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga
    17941 ggaaacacga cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta
    18001 gcaggtgtcg aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct
    18061 agtgccatgc tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt
    18121 gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc
    18181 aatttcaccg atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca
    18241 gacaaaggaa cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact
    18301 ttttgtaaga taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt
    18361 tgttttaaag aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg
    18421 ttcgcttttt tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact
    18481 tctgatcggt ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta
    18541 cgtagttatg ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt
    18601 ttcaaaatca gactctggac aagaaacagt caactataga tatatcctgc ctggctatac
    18661 gaaagagggt aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa
    18721 aggagcatat ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt
    18781 cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa
    18841 atgaacaaaa tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa
    18901 aagacgcaag cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc
    18961 atgggcgctt ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa
    19021 ccaacagatg ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa
    19081 ctagcagatt ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa
    19141 agcttgacag cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa
    19201 gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa
    19261 agctatcctt ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg
    19321 atcacacaac caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt
    19381 gcccgggatc ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt
    19441 cttgtgacgc atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat
    19501 ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa
    19561 atactatcta ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt
    19621 atcacttact gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt
    19681 tatggcatct gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa
    19741 aagtaatacg atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg
    19801 gatcatcaca ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa
    19861 agactatgca atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt
    19921 ttctgaaaca ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg
    19981 attgacaagt atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt
    20041 tacacctttt agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt
    20101 attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg
    20161 tgcggatcag acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt
    20221 actgggaatt gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca
    20281 agtctttggt ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca
    20341 ttctgtgatc attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa
    20401 attgaacaat ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct
    20461 ctcaatggtc gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt
    20521 taaaaatgaa attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa
    20581 tcctcgggca gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta
    20641 tactcaaaaa gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc
    20701 attgatcatg ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc
    20761 tgctaaaaaa atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga
    20821 acagcaagaa aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc
    20881 acaatccact ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga
    20941 aacacttgtt gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa
    21001 ccagaaatat gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg
    21061 attcttccta ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt
    21121 gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg
    21181 cgggttgctt aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat
    21241 cttaggtgta gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc
    21301 atacaaagat atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt
    21361 actgacgaca tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc
    21421 tgcatcaagt aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg
    21481 gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca
    21541 atatttttgt cacaaccttt ttttcacggt cttttattag aatatagtaa attttttctc
    21601 gttactcacg gaataagctg ttaagaaaac ggtttccatg tatactggaa tcagtgcaaa
    21661 tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa caaaattttg gcgaaaacta
    21721 tcacttgatt acgatcacga atcaaaatgg gacatctttg tctgtcagtg atcttggggc
    21781 acgggttgtt cattttcaaa caataatcga taatgaaaaa cgagagttga tcttaggttt
    21841 tgatgaagca aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat
    21901 ggctggacgg attacgaatg gcacattcaa attagatgat gaaacgttcg aagtaaaaac
    21961 agacccggac actggtcatt gtttacatgg tggtgcacct agctttgaag ccaagcagtg
    22021 gcattataca gtcatcaatg gtgaaaacga agcatcggtc attttctatc agactagccc
    22081 agatatggaa aacggatttc ctggaacttt agatgtagaa gtacggtata cgctgacgaa
    22141 cgatgataca tggcgagtga cggtccaagg gatcagtgac cgcaaaacag tattcaatcc
    22201 tacaaatcat gtctatttca atttaaccgg tgatccagca cagtcaatcg acgaacatat
    22261 actttggttg aatagccgat tatttgctac gctcaataaa gatacaacgc ctacaggaga
    22321 agtagtttca gtaagtggca cagcatttga cttccaacgt cctaaaaaat taggtgaggt
    22381 ctttgccagt gattttcgcc aatcaatgat agtgggcggc atcgatcatc catttttgtt
    22441 gaaaaaacaa tctggtttac taaatgctgc ggaattgaca agcccagatg aaaaagtatc
    22501 gatccaagta aaaacagatg cttcaggaat cgtgattttt acagcaaatt ttggagaaga
    22561 tggaccggaa atgcgcggga ataaattagt aaatcatggc ggtatcacat ttgagactca
    22621 agaactgcca ggagcggagc gacttgaaag ttttggagat gtcactctgg aacctaacca
    22681 agtacgcgag tttgtaacgg aatacaaaat aaatataaag aaatagaata aattaaaaag
    22741 ggagtgtgac agtgtcacat ccccttttta atttattcta tttctttaaa ggcagatcaa
    22801 gccagaatga tttgtctaaa cttaacaagg ttagtacagg aacagttgca gctttaactg
    22861 tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt gttccacaaa tcccagattg
    22921 aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt tatcgttgaa aattctaatc
    22981 ctgtgattgg cacgccatct gttgtccatt tgattggcac atcgaatcgc ttagacatat
    23041 tgaggatttc agcgtttgct tcttcagttg ttgctgcttc ttttttcggt tcatcatatt
    23101 ttgtcaggtc gtacgcttcg atgatcgcgt tcaatttttg tgaatattgg cgatctgtag
    23161 cataacgtcc ttccaaatat ttggttgctt ctttgtagtc ttttgtttcc gacttccaag
    23221 ttggtttgta gaaatcagga ttgccagtca gtccattttt gatcaagtcg gcatagtctt
    23281 ccaaactttc cttgtaagaa ggatatttac ggaattgtgc gcggatttgg tagctttgtc
    23341 cggaactatc ttgttcaagt gtattgaagg tcacgctgct tcctttatat gatcctttga
    23401 taccaaaaag gttatgattt ggttcgcttg ataataagct gtttccagat ccactttcaa
    23461 ggatagcttg agccatcatg actgatgcgt agattccttc tttttgcccg atttcctgtg
    23521 catcatctgc aatctttttc aagaactgat cagttgtttg gttttgactg aaatggaacg
    23581 agccatctaa tccatctaaa atagatgagc cgcctgtcag cgaacctgaa gcagcaggta
    23641 aacctgctaa gtttactcgg acagcaaaat caggcgtgaa atctgaaata gaagttactc
    23701 gaactttatc tcctggttga ggcgcatgga taaattgtcc gttcccaata tagataccta
    23761 catggtgagt aacaccggaa gaaggtcccc agaagacgag gtcgccaggt tgggcctgac
    23821 ttactggaat ctgcgtacct gcatattgct gttcgcctgt ccagccaccg atataatgac
    23881 cggttgcttt catatatacg taataagtca aaccagaaca gtcaaaagta tttggacctt
    23941 ttgctcccca tacatatggt tttccaagat gcttgaatgc ttctcgaacg attgcttgtt
    24001 ggacattcgt tgtatttgta ggcaatacag catcaattgg tgattttaca acaaaatcag
    24061 aagaagcaga agatggattc tctataccgc tttgatttgc tgtatttgga cgagatgaag
    24121 cattgttcgt tccgccattc gatgaaccat tgtttggatg gtttggttct ctctgagtat
    24181 ttccattgtt attcgatcca gatgggtttg tttgattcga agaattcccg ctgttcgaat
    24241 tattgttatt attgtttgaa ttatttgaac cattattatt gttgttatta cccacatctg
    24301 gtttgtttgg cgtttccgga tctggtgtac tagggacttc ttcactagga tcagttggtt
    24361 tctctgttga agtatccggt ccagctttaa attctgcagt aatagataaa ttgttcgtac
    24421 ctactgtaaa ggtctttgca tcataattta cttctttggc accattaaca gcgactgaag
    24481 caatttgttc accttcagct ggcgtaatag tgtaagttac cttcgtttcc gctaataaac
    24541 cttctaccgt tccactttcc gtaatatcca aaggttgctg ttcaggggaa tccgctgtcg
    24601 aagcagtcag ttggatctta ccaatcaaag aaggagcaat tgtaagggaa tatgtctcgg
    24661 ctggcttttc attttctgat ggatcagccg gatcaatttc agttggctcg gtcgatcctg
    24721 ttgttgagtt agttgttcca gttgggtctt ctgtaaaaga atccgttgtt tcactagtag
    24781 ctgagctgga tgtagtcgta tctgttgtga ctgtttgatc agctatttga ttatctacgg
    24841 caaatacggt attggtcgac aaaacagacg gagcaatcag ccaagtagca gctagcaaac
    24901 gtatcgtttt tttatttttc ataataaact agtccttttc ctttcattga ttttctgtct
    24961 tattcagcat tcattagaca ttttttggaa cagataatcg tgtcgaaaag ctttttcttt
    25021 tcataaatct gtctgtgtat tctaaataaa acaatttgta aataagcaac gagcattcat
    25081 tcgtttttct ttgacgttca ttttattgca tagaccacct ttgtttgggg ataaacaagt
    25141 ggggataaaa atggttatgc atatgtaaat tataccaaag tttggaaagt atcacaaaaa
    25201 tttataaaaa taatgatcct aaaaagaaaa agactgaaat atgtgataaa catctcatac
    25261 ttcagtcttt ttcattaact attcataaga cattaggtct tcatcaaacc cgctaacgag
    25321 agtagtgatc attgctacga ctagactaac agccagtcct gctaagtacc agaaaaaagt
    25381 accggaaatg aatgcaggca atgtggtcag actaccaaat acaaaagcga tcgctttcac
    25441 ttgggcaaat cccattacag ctccaccagc agctgcacca atgcaagcag caacaaatgg
    25501 ttttttcaat gggatcgcaa ttccgtaaac agttggttcg gtcactcccg caagtaaaat
    25561 cgaagcaaaa gcagaaccgg agatcgaacg ggtttttgaa cgtttacttc gcagaaatac
    25621 acctaaagct gcaccagcca ttccaaaatt ggctgctcct gtcaacggaa cgattcggtc
    25681 gaacccataa gcagagatat tactgatcat gacagggttt acagcccatt ggataccaaa
    25741 tattaccaaa atattccaac caccagcgat gattgcgcca ctgatcggtc cgttaatatc
    25801 catcatccaa gtaattgcgg cagctaaacc ttcgccaagg taaacacccg caggcccgaa
    25861 taccattaac gtcaaaggaa ccgtgataac taatgaaagt aaaggtacaa atactaattg
    25921 catattttcg cgaatatatt ttttgaggaa tttttcaaca aaagaataaa agaaaatacc
    25981 taaaatcgcc ggcatgacag tagacgtata ctgcatcagt actacaggaa tccccagaaa
    26041 atcagtagct gctttgctac tgtctgaaag taaagaggtc agattcggtt caatcagtgc
    26101 tgcagcaatg acagccgcaa tatatccatt cactttaaac gttttggcag cagaaaaagc
    26161 aagaaagatt ggcagaaaat aaaagacggc attcgacgca gcagacaaaa tatgataagt
    26221 tccgcttgtt tcactaagcc agccaatcgt agtcgctaaa acaaccagac cttttaatat
    26281 accagaaccg gcaaacagcg gaatgatcgg cgtaaagatt tccgatatca aaccaaatag
    26341 tttttcgatc ggatttagct tttctttcgg agctgaatga tcgatttgac gcgtcaattc
    26401 cttgtaagca ggagccaatc cctgatccat gattatttct gcatatctat cgagtaaaat
    26461 gaaatactta atggaaggga tttgctgttt taatttttct gcattggcta ctggctgttt
    26521 tttcaatttg atacgaagcc gagtcgcaca tggaaggaca gaatcaatat tgtcttttcc
    26581 tcccaactgt tcaatcagca tatgaatctg ctcggacagt accatctgtt caggattcat
    26641 tgtcgtttcc attagaagac cactccactc actaatataa tattggcgta aggtgtcatt
    26701 tcgcctgtac gaataaatgc atgacagtta ttcaactctt ttttcatttc ttcatgtggg
    26761 ataaagctaa tcggtgtatc tggtaacaat aatttgattt tttccaatat ttccgggttc
    26821 attgtcttta tttcttccgc caattcaatc gattgtacct caagttcttc taaaacgttt
    26881 tctaaaacag ataaaaaact tggaagaccg tttgagacag ccaaatctat tttttcagta
    26941 ccaaagggga caggcatgcc tgcatccccg atacttaatt tatcaaagtg ccccatttgt
    27001 gcgattactc gtgaaatatc tgaattgatt acttttcctt ttttcatgtt tgtttcttcc
    27061 tctcattgtt aattttagac tgctgattgt tctaaagctt cgcggtaagg gatagaaggt
    27121 tgcgcaccat aagactgcac agcaacagaa gaggctagat ttccataaag gatcgcttct
    27181 ttcaagttac taaggtctgg ctgaagtgtg ctgcttaatg cgccaataaa tgtatcacct
    27241 gcggctgttg tgtcaacggc atccacttta aaagcaggga cgataccgtg ttctttttct
    27301 gtatgataga aagcgccggc actgcctagt gtaatgatta ctgttccaat acctaattca
    27361 tggagttttt cggctgctgc gaccaaactg ttatgatctg tcactctgat tcctgtaatg
    27421 atttctgttt ctgtttcatt tggtataatg atatctgtct tttcaagtag ttcagtagga
    27481 atcgtttcac gagctggtgc aggattcagg atcgttgttt ttcctgcgtc ttttgcaata
    27541 gaaaaagcta ggatcgtcgc atctaagcta ctttcaaact gagcaataat aaagtcactg
    27601 tttgtaatca gttgcttgtt ttttaggact tcttgttcat ggaaagcatt atttgcccct
    27661 gcatggatca agatactatt ttctcctgct gcgtctacca taatcattgc ttgtccggtt
    27721 gtggcgcctt ggatttcagc tatcccagat gtatcgatgt tttcttttgt cagtagatct
    27781 aataactgtt gtccttcgct gtcagcccca acgcctccaa taaaagatgt ccgtgcgcct
    27841 gaacgtttgg ctgccactgc ttggttggca cctttacctc cgccagcgtg aaaaatctca
    27901 tgcgcatgca tcgtttcgcc tggcttgggc atgttggtta aacgaagggt agtgtccata
    27961 ttaatacttc caattactgt aaccgtgttc attttacaaa cctccaacta tctcatttat
    28021 tttgatggga aaattatttt ttaaaacgtt ttagtaaaac gttttaatag ttcgataaaa
    28081 atatagcaca ccttgaaagc tatttcaagg gtgcagttga aaatcttttt tctaatcgta
    28141 caggtaatct tttttcttcc cattcttttt ctgggaattg gatccgatct aacaataact
    28201 tggcagatgt ttgtcccaat tcgaagattg gttgtgcgat cgttgtcagt ttcggcttga
    28261 tatactcgca catatctata ttatcatagc caataatgct gtagtcttca gggatgcttt
    28321 ttccagcttc ctctaatccg cgatataatc caaaagctaa ttcatcattt aaagcaaaca
    28381 caccagttgc agcagaatca agaagttctg ctgtgatctg gtagccacct tgttttgaga
    28441 actgtgtagg taagaggatc agttggtcgt gagggatttg gtaaacatca agagcagatt
    28501 taaacccctc aatccgagcg tggacatttt ccggtggatt ttcaggatag acaagggcga
    28561 tcgtttgatg tcctagacta agcaagtgca tgcccgctag gtaaccaccg cgaaaatcgt
    28621 ctgttcgtac ggcatcacta aatccttcag attttttctg atccaaaaca atgaagggcc
    28681 ggccttgttt tttcaaattt tcgttgattg catctgtaga gacagcagaa gtcgcgataa
    28741 tgaatccatc cacacctcga cgtgtgagct cggctagata ttcgatttct ttttgatgat
    28801 cagaatcagc attacataat atcgtgacga aattctgctt atacaagata tcctcaattc
    28861 ctcgcatcag tgtactgaag aatggattcg tgatatcagg aacgagtaca ccaattgttt
    28921 tcgtttcctt ggtaatcatt tgtctagcaa aatagtcagg ctgatagcct aattcttctt
    28981 tggcagctaa aactttctga acagttttcg gactgaattt ctccgtattt ccatttaata
    29041 tcaaggagac agttgtgatt gaaacgccgg aaagtttagc gacttctttg attgtgatct
    29101 tttttttcac catgttcata ctcctatcta aatcctgtgg ttattttccc atgaaatcaa
    29161 aaagaagtaa atgaaaatca cgtgtgccag tacttatttg ctttgttgga tagcttcgat
    29221 tatttgtttc gttgagcgta ctcgtgcagt tttcggaaat atctttttta tgaacaattg
    29281 atgggattct ttgtcacgat cactgcaagc atcttcagca agcacgatat ggtacccgtg
    29341 ttgaaaggca tctaatgcag tagcatatac accgtttgtt gtcgctacac cgcttaaaat
    29401 gatagtttcg attccacgcc ggcgaagttg aagatcaaga gaagtaccaa aaaaagcact
    29461 tggattgtat tttgtgatag tcaacatatt atctgtatct ttcaaagaat ccttcaaaag
    29521 taaatcagtg aattctggag gtacagaagc agtcattcca cccatatcag tttgttgact
    29581 caaataaggg aaagtggaga tgtctacgtt cactaaaact ttcaaggcag cggtacgttt
    29641 caatgttctt actaacaaat cattgtttgt cagaatagat gctgcagtgt gtggagagag
    29701 tgtaccgtta tttacgatct ctttttgcat gtcgatcgcg acaaaagcag tttttgataa
    29761 atcaaaaatc atattttcct ttaaatcatt catgatgttc cttctttcaa ataacaatgt
    29821 tttttatgta agtaaatcat aaatcgttta tttgtcaact aaattaaagg attcctttct
    29881 ttagtctaga gaaattcagt ggtaaaataa ataagtgtga gaaaacgcat tcgatgcata
    29941 gatatagcga gaaatatcgt gaaaatggag gaatcagagt gaagtatgtc gaagtaaaaa
    30001 atgaatacaa acgatacaaa atgggagaaa cgaccattac agcaaatgac gatatttctt
    30061 tttcaattga aaaaggcgaa ttagtgatta tacttggtcc aagtggagca ggaaagtcaa
    30121 cgattcttaa tattttaggt gggatggata cacctgatga aggtcagatc attattgatg
    30181 acacagatat tgcgcaattt tcagataaac agcttactgc ttaccgacga acagatgtag
    30241 ggttcgtttt ccaattctac aatctcgttc ctaatctaac agcaaaagaa aatgtggaat
    30301 tggcaacgga agtttctcct gatgcgcttg atcctgtaga ggtattgagg caagtgggcc
    30361 tagctcatcg attgaataac tttccgtccc aattatcagg aggcgaacag cagagggttt
    30421 caattgctcg tgcactagct aaaaatccca aacttctttt atgtgatgaa ccgacaggcg
    30481 cgctggattt tgaaacgggc aagcaagtat tgaaattatt gcaaaacgct agcagacaac
    30541 acggcaatac cgttttgatc attacacata attctgcttt agcgcccatc gctgatagag
    30601 tcattcacat taatgatgct aaagtaaggt ctgttgaatt aaatgatcat ccttcatcca
    30661 ttgatgagat tgtatggtag ggggatttta tgaaaacaag aacatatctt aaagcaggtt
    30721 ttcgcgaaat tcggcaatct aaaggacgat tcattgcaat catccttatt gtcatgctag
    30781 gaacactttt atttgtaggt gtcaaaactg ctggacctgt catgcaaaaa acgatggatc
    30841 actatgttgg tacagcagga ctttctgatt tgcaaatcgt ttctactggt ggcttgacac
    30901 aaaaagatat tgctcaagca gaaaagatcc cagatgcaaa agtagaaaca ggaaaacaga
    30961 tctattattc caatactggc aaaaacgaag tgattcaaat attttcttat aacaaaaata
    31021 acaagcagaa caagctacaa ttaactgatg gaaaactccc ggaaaaacca aaccagcttg
    31081 tattggatga aaaagcggaa gatgaagggt ataaaatcgg tgatatttat cagattgatt
    31141 cagatgaatt gaaagaaaaa gagtatacca tcacgggctt tgtccgttct ccattgttca
    31201 tcaacaattt ggagagagga tacgcgaacg tcggaaatgg tagcgtcgat tattttgtct
    31261 atttacctga atccgttttt aaaacagata tttattctgt cttatatctt gattttacaa
    31321 acgtaaagaa tttgaatacg tacagcaaag cttacaaaga taaaatggag caaaatcaag
    31381 aaaaggcaga aaagtattta caagatcgac ccgaagaacg cttagaagag ctgaaaaaat
    31441 cggccaacga agaattagaa ccggcacagc aaaaaatagc ggatggcaaa gcacaaacaa
    31501 cgcaagcaag aacacggtta gaagatgcta aaaaacagtt agcgcaacag tcagcaacta
    31561 tccagcagct tcctgaagcg caacgaacaa tcgctgttca aacattatct caacaagaag
    31621 ctcaacttgc tgaacaagaa aggcaattag ttgagaaaga acgtgaactg gcctctgcag
    31681 aggaagaatt gaatcgaaat gcagaaaaaa ttgaaaattt aactaaacca tcatacctat
    31741 acaatgaacg ttcagaaaat gtcggttttc aagaatttgg tgatctgtct gatcgtatcg
    31801 ctgcgattgc caatgttttt cctgttttct ttttctttat tgccgcattg atcaccttta
    31861 cgacaatgac acgtatggta gaagaaaacc gcagggaaat cgggacatta aaggcgttag
    31921 gttatacgaa agttgaaatc gcaaaaaaat atgcaatcta tgcaagtcta gcctcaggga
    31981 taggtattgt tttaggaacg atattaggaa cgaatctttt gccaaggatc atttttgagc
    32041 tgtctaacga gcggtatgat attgggagtg cgcttatttt ttatgactgg ccgccgattt
    32101 tacaagcagc tgccgcattc ttcattgccg catttggcgc tgcaatgatc gttttattca
    32161 aagatttaag agaacggcct gctgcattgc ttcaaccgaa agcaccgaag ccagggaaac
    32221 gcatttttct tgaatacatc actccgctgt ggtcacgcct tagctttaat caaaaaatca
    32281 gttaccgaaa tctcttccga tataagtcaa gaatgcttat ggcgattatc ggaattgctg
    32341 gatgtgccgg gctgatggtt gcaggcgtgg gattgaaaga ttctctaagt tctgtttcag
    32401 ccaaacaatt tggaccgatc attgactatc aagcaatcgt tacattggat tcatccacgg
    32461 aagatagtcg tcaaaaagca gcggatatgc tgcagaagca agaaaaagta aatgagatcc
    32521 tagctattca tactcaaacc gtagaattga gaaaaaaagg acatgcttcg caaagtctga
    32581 cagtgatcgt accaagtaaa acagctgatt tggatcgttt tatccattta actgatcttg
    32641 aagagaaccc acgctccttg cctaaaacag gaatcgctat tacgcaaaaa gtagcagaac
    32701 tatttgactt atctacagga gatcatattt cgctttatga tgaagatcaa aaagaatgga
    32761 aagtcgaagt aaaagaaatc gtgaaaaact atctaggtag ctttatttat atgtcccctg
    32821 aatattatgc ttcagttact gatcaatcga tgatggataa tgcatttttg ctgcgtacag
    32881 ataagatgac aacaaaagaa gaagaaatgc tttccgagaa actgcttgcc acagacgctg
    32941 tgacaaatac gtctttcatg tccaaacaaa tcgaaacgca agaagaatca atggcgaact
    33001 tagactcggt tgtcttaatc tttgtcgtat tatcaggact gctggctttt atcgtcttgt
    33061 ataatctaac taatatcaat atttccgagc gtgtacgcga attatctaca ataaaagtcc
    33121 ttggattttt tgataaagaa gtcacgatgt acattgttcg agaaaatatc atctttactc
    33181 ttttaggaat catcggagga tttggtgtag gatatatatt gactgacttc atcttgcaac
    33241 aagcgtctat ggagaatgtc atctttccat tagtgatcac atggtgggct tatgtcttat
    33301 cagcaggatt aactatcgta tttacggtta ttgtgatgat tgttacccat ttcaaattaa
    33361 agcatatcga tatgattgat gcgttgaaat cgaatgaata aaaacagagg ttgtgaaatc
    33421 agcggtttga cctgagtatt agagaaacaa acggcaaaat agtttttcat attttgccgt
    33481 tttttgagct aataccgaag gacagctgat tgaacaccat gaatcagagg ttgtaaaaaa
    33541 gccgtcctgc atcgagcaaa aagaatactc aaataaaatt agctcctcat gttttacatt
    33601 tgagaagact gattgccgta gataaaagct tttgaacacc atttatagaa gaaacaagaa
    33661 tattttgcta ttttaaacaa gaggatagac aaatacatgt ctgatctctt gttttttatt
    33721 gggcaacaga cctcttagaa tgtataaaga taaattttac aaacgctttc ggttcagtta
    33781 taataataaa tgataacttg tatatataag ttgtggtagg taataaatca gttgaatgga
    33841 ggtaaatatc catgacattt aaagacaaag tcatttacca aatctatccc aaatcttaca
    33901 aagacagtaa tggtgatgga attggagatt taaaaggaat caaagaaaaa ctcccgtatc
    33961 tgaatgatct aggcatcgac atgatttggc taaaccctat ttatccaagc cctcaaaaag
    34021 acaacggtta tgatatctct gattacacag ccatcgatcc tatctttggt acgatggagg
    34081 aattcgaaca gctaatagcc gaagcaaaga aataccggat agaaatcatg ctcgatatgg
    34141 tgctgaatca cgtttcgaca gaacatgaat ggttcaaaaa agcactagca ggggaccagc
    34201 attatcagga cttcttcatt ttaagagaag agccaacgga ttgggtatca aaatttggtg
    34261 gaagtgcttg gtcgccattt ggagatacgg ggaaatatta tttgcactta tatgatcgga
    34321 cacaagctga cttgaactgg cggaaccaag cagtaagaga ggaactattc aaagttgttc
    34381 gtttttggat ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat gttatcggta
    34441 aagacgaaca attgaaaaat aataaagaaa atgatggcaa acctgaatat acggacaaac
    34501 caatcaccca tacgtatcta aaagaattga accaagcaac gtttgggacg gattccacta
    34561 tcatcacagt cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg tatacagaac
    34621 ctagtagaga agaattatca atggtcttta atttccatca tttgaaagtc gattatgaaa
    34681 atgggaataa atggtctact cctccatttg attttgcaga attaaagcga ctatttcata
    34741 catggggaga aaagatgagt gagggtaacg gctggaatgc tctatttttt aataatcatg
    34801 atcagccacg tgcgctcaat cgatttgtag ctatcgatca gtatcgaaaa caaggagcag
    34861 agatgctagc aaccatgatc catttgaacc gtgggacgcc ctatatttat atgggagaag
    34921 aaatcggcat gatcgaccca gactattcat cgatttccga ctatattgac gtagaaagtt
    34981 tgaatgctta taagttactc cttgaacaag gattcacaga aaaagaagcg tttcgtcgga
    35041 tcaaagcaaa atctcgcgat aactctcgaa cacccatgca atggtcagac acagaacaag
    35101 caggatttac tacagggaaa ccttggttaa aagttggagg caagctagaa gagattaatg
    35161 tagaaaaaga actatcttct gaagattcta ttttgtccta ttacaagaaa ttgattcggc
    35221 tgagaaaaac atatccaatc gttgcacaag gagattatca tgcttatgaa gcaaatcatc
    35281 cggaagttta tggctttctt cgccaatttg aaggacagca gctgcttgta ttaacaaatt
    35341 tctatgataa agatacaagg atcgccatcc cagaaggatt cacagaagcc aatattctta
    35401 tcgataatta ttccgatact gttcttcatc aaagtctgga gctaaaacca taccaggcaa
    35461 ttgctttgct gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag ctattctcgt
    35521 aagatcgacg ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa gagttttcat
    35581 actctccttt atcgccataa aatttattgt agcgttgttt gaatttacgg aaaataaaag
    35641 taacgagtaa tttgactaac gtatagatag gaataccaaa aataacgccc acaattccca
    35701 ttagatcacc catgacaagt aaaacgatta agatcgtaat cggatggatc tgtaaacgat
    35761 cacccattac tcgaggaatc accaagtcac cgtggagaag ttgtacggca aaccagacaa
    35821 tgacaaattt gacagccata aaagtagagt cttggaatgc aatgatcaaa cctggaaaga
    35881 aggcgataaa cggcccaata tatggaatga tacataacaa cccagcagaa atagctaaaa
    35941 cactagcaaa ttctaatccg atcagtaaga aacaaaccca gtagactgct ccaaggataa
    36001 atgaagcaat gatttgcccc tttagaaatg aacccaattg ttgattagca atctttgtca
    36061 aatttttaaa atctgtacga aatgctggtg ggacaatcgc taaaactgac tgataaaatt
    36121 tttgaggatc tctcaaaagg aaaaaagcga caattggacc agtaaataaa gtaataaaaa
    36181 tagctgtaac agttgaaaaa atgttgctga tccgttgtgc gccactttcc caataatttc
    36241 caataaatcc agctatatca gacgtcacgt cgtcaataga agcgaaaaat tgattaaatg
    36301 aatcggcaaa aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa tcttgaaaca
    36361 aatctggcaa actactgatc aatgtctttg cctgttcttc aagaaaagga ataagccata
    36421 tgcttccaag ccccaataaa aataaaataa tcacataaac gataatgact gacaagttcc
    36481 tcgaaaggtg cttgctcaaa cgtttgatga ctggttccaa aagataatac aagatcagtg
    36541 caaacaataa tgggggcaag atagtagcga taatgattcc gacaggcttg aatataaacg
    36601 agatctgatg gaaaaaataa atgatcagcg caagtaagat aactattcct aaaacatagt
    36661 aagaggtttt tcctcctaaa aagcggatca aacgattcgt tgtatctgtt tcttttttca
    36721 ctctttttcc tcctttatct ataaattcat tatagacaaa agcgggtata aaaggaaaat
    36781 gatagaggtt gattgcaaaa atatcgtcaa tcattcatat aaactttctc atcaattaaa
    36841 ataagaaaac gcttgacagt tcttttaacc atatgataaa ttaaatcctg ttgctaaaac
    36901 gtaactatta cggtttgcga aaagcgaatc ggaacattca aatgaaagaa gatgagaaga
    36961 ggagtgtcat aaatgaaacg attgataggt atattggcca tgctggtttt agcaggtatg
    37021 ttaactgctt gcggggcaag tgggaaagcc gaggacagta aagaaaaatt gagcgtgatg
    37081 acgacctttt atcccatgta tgattttaca aaagcaatcg tcggggatga aggagaagta
    37141 gagttattga ttccagcagg gactgattcc cacgattatg aaccttctgc taaagacatg
    37201 gcaaagatcc aagatacaga tatctttgtt tataatgatg aaaacatgga aacatgggtc
    37261 cccgcaatcc aaaaaacatt acaagaaggc aatgtccata cgataaaagc gacagaaggt
    37321 atgcttttac ttccgggaag tgaagaagga catgatcatg accatgaaca cggggaagaa
    37381 ggccatacac atgagctaga cccacatgta tggctagctc caagtttagc aatcaagcag
    37441 gtagcaaaca tacgtgatca gctgattgaa gcttacccag aaaaacagga agtatggaca
    37501 aaaaatgcag cagcctatac cgagaaactt caagcactcc atcagcttta tcaagaaacc
    37561 tttaagcaag caaaacaaag atcttttgtc acgcaacatg ctgcattcaa ctatctagcg
    37621 ttagaatatg gattgaatca agtatcgatt gctggtcttt cttctagtga agaaccgtct
    37681 gcggcgagaa ttgctgaact gaaacacttt gttaaagaac atggaatcaa ctatatttat
    37741 tttgaagaaa acgccaaaga tagtattgcc agaacgttag caaacgaagc aggtgtatct
    37801 ttagaagtat tgaatccttt ggaaggattg acgaatgaac aaatagaaaa tggagaaaac
    37861 tatctttcta tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga gacagaaaac
    37921 ccgctagaag atactttgac ccccaaaaaa gaaaagagcg tctacaacgg ttattttgaa
    37981 gacgaggatg tacaagatcg ctctctttca gattgggcag gaacttggca aacagtagat
    38041 acgtatgtag aagatgggac ttttgatcct gtttttgaat ataaagaaaa attgaatcag
    38101 gataagacag cacaagaata taaagaatat tatacgaagg gctatcaaac agatattgct
    38161 tcaatcaaga tcagtgagaa agagatggca tttacgttta agaatggaac aacgaaacgt
    38221 tcaaattatc gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa ccgtggcgta
    38281 aggtatctat tcgaagcaga agataaggaa agtggcgctt ttagatatat tcaattcagt
    38341 gatcacacga ttgcaccggc tgactccgaa cattttcatt tatatgcagg aaatgaaagt
    38401 caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga tacaatggat
    38461 ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa ccgaagggtt
    38521 tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga aatcaatttc
    38581 tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac ttctctggtt
    38641 tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg acaacggata
    38701 aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa ttgggcatga
    38761 gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata ttggatgtct
    38821 ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc tttttggatt
    38881 tgggacattt gaaaatcttc gaaaattttg ctcaggatat gctcgacatt ttcttttaaa
    38941 tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt taagattttc
    39001 atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc taattcacgg
    39061 atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat atctaaaaag
    39121 taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc cggatcagag
    39181 gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt gatcaccgtt
    39241 gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat catatcatta
    39301 ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc aattgatgtt
    39361 tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa gccaaggcct
    39421 tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt taacggtaat
    39481 gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt gagtatggtc
    39541 aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc caagataata
    39601 ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg ctcaggaaaa
    39661 tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga cagtaaacta
    39721 cggatctcag gaactttcat attatccaac atacttagat tggccggaaa aaggttcatc
    39781 atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc taagtcgcct
    39841 tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt gaattctctc
    39901 aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct gacttgttgc
    39961 tctaataact gatcagctaa tttcttatta gagattttcc aataataaac caagatgatc
    40021 agggcaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac atcaggtgtt
    40081 tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat caatgtcccg
    40141 atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga tttttttaaa
    40201 caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata ctgcaatgaa
    40261 tctcctggtt caataaccga agatttcaaa acaaaaaaat cgccgatgct tgttaagtga
    40321 tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg ttgacgcgaa
    40381 gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc agtaaaaatc
    40441 cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac tcccgcaaac
    40501 agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta aatcaaatat
    40561 tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt accaaattat
    40621 agcacaattg gcgtattatt tttttaattt ttcagggatt ttgaattcag aactcatacc
    40681 aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa tgattgtttt
    40741 tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc aacgtagcta
    40801 tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag aaaaataacg
    40861 taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg ggacgtttct
    40921 cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt aacaaaaagc
    40981 agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga tgtttcatca
    41041 ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat tcagagtcgg
    41101 aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg attcctgttt
    41161 ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt ccaagattat
    41221 tcaataaaac ttctaagaca aattttactt ggacatacac catatcttct tccgatttgt
    41281 ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc tcatacgtac
    41341 ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca taaaaagtgg
    41401 ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa gaaccttacc
    41461 aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt gctttttcga
    41521 gtgatttatg ttaaaaaatt actgacctta taaaaggtga tggagggatt cttatggaaa
    41581 atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta tttgaatcac
    41641 caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt gtcatgaaat
    41701 gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt gtacgcaaat
    41761 caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag gttgtgagtc
    41821 tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca tgttttatga
    41881 atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa aaagggctgc
    41941 gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg gctactttta
    42001 gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa atgaagtagt
    42061 aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca ttgttgcagg
    42121 aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt tagaaacaaa
    42181 acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt ctaatccaga
    42241 aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg caatcaaaga
    42301 tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata gtatgctgtt
    42361 tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt atgaaggtct
    42421 tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac cagatctact
    42481 ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa gaggccggga
    42541 atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac tcaatcgacg
    42601 atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg atggggacaa
    42661 atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa tcaagatgcg
    42721 ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta caaataaccc
    42781 gaaagcaaag ggaatcttta caactaaata atataaaaac taagcaaatt gctcactcgc
    42841 agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag atcaaatagt
    42901 gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg ccatgatcaa
    42961 aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac agaaaacaag
    43021 gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa atcgatcaaa
    43081 atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc cttttaaatt
    43141 tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag ctacccactg
    43201 gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat ggacgggtgt
    43261 caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc gccagaaatg
    43321 tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc ctaagaaaaa
    43381 gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa atgcttggat
    43441 gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt ggatataagt
    43501 ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac gcccctcgtg
    43561 aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc cttatcttag
    43621 cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta aaatcatatc gcttcccatt
    43681 ttcattttta gaaaggtctg ttagtataaa gaaaaaatag acaacgaaac tttaaataac
    43741 caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc agttgaatga
    43801 aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt ataaaaagct
    43861 tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg cttatggcga
    43921 agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa acagctcttt
    43981 ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa aagcagctat
    44041 cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg gcaaaacgca
    44101 aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg aagaacgcat
    44161 actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag tagataattt
    44221 cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt ttaggatata
    44281 caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag gagacttttc
    44341 aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta aatataatct
    44401 ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact attggcagaa
    44461 tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg attccattga
    44521 taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg aactccaaga
    44581 acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc tgaaaagcgc
    44641 tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc tattcattga
    44701 tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga caaaggaaaa
    44761 caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat tcagaggatc
    44821 agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc ttttcttatt
    44881 aactaattat cgttctcggg cagctattgt ccaaggagca aatagattga tcagctataa
    44941 taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag gtgttatgga
    45001 agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac aaattcagac
    45061 atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat cagcttcaca
    45121 aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac tggaagcaga
    45181 tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc aaaaaattgt
    45241 tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc aattgctccg
    45301 gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact tttctcctaa
    45361 atatcaaaat atagccgttg cagattatat tttgtcgata agccccaatt taaaatcgaa
    45421 atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc atcaagaaat
    45481 ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa tcgaccagag
    45541 tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa gactccaagc
    45601 cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga ttgcttacag
    45661 acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag gtcttagtta
    45721 ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg gagcgacaga
    45781 aaagaaagta atcgacttta atgaaataaa agcagaacca gtaacgacga tagaagaaga
    45841 acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc tatgtgtacc
    45901 tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa ccgggagtcg
    45961 tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga agtggtcagt ttcaagaaag
    46021 aagaaggaat gtacgaaaat tgcttctcaa atttttgtga aatttcagct tattttcgaa
    46081 gaagctattt ctggaacaca gtttattcgg aaaagggagg tgtgacaaga attttgtcac
    46141 acctc
//
LOCUS       DAZUMO010000017        45515 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000017 DAZUMO010000000
VERSION     DAZUMO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.017"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..976
                     /gene="thiD"
                     /locus_tag="ACG8ER_000916"
     CDS             161..976
                     /gene="thiD"
                     /locus_tag="ACG8ER_000916"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS5049516.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1181..2632)
                     /gene="cls"
                     /locus_tag="ACG8ER_000917"
     CDS             complement(1181..2632)
                     /gene="cls"
                     /locus_tag="ACG8ER_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5049517.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            2974..5640
                     /locus_tag="ACG8ER_000918"
     CDS             2974..5640
                     /locus_tag="ACG8ER_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5049518.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPVIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6004..6756
                     /locus_tag="ACG8ER_000919"
     CDS             6004..6756
                     /locus_tag="ACG8ER_000919"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5049519.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6778..7932)
                     /locus_tag="ACG8ER_000920"
     CDS             complement(6778..7932)
                     /locus_tag="ACG8ER_000920"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS5049520.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8117..10558
                     /locus_tag="ACG8ER_000921"
     CDS             8117..10558
                     /locus_tag="ACG8ER_000921"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS5049521.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(10888..11592)
                     /locus_tag="ACG8ER_000922"
     CDS             complement(10888..11592)
                     /locus_tag="ACG8ER_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049522.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(11779..12024)
                     /locus_tag="ACG8ER_000923"
     CDS             complement(11779..12024)
                     /locus_tag="ACG8ER_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049523.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12041..12892)
                     /locus_tag="ACG8ER_000924"
     CDS             complement(12041..12892)
                     /locus_tag="ACG8ER_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5049524.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13058..14062
                     /locus_tag="ACG8ER_000925"
     CDS             13058..14062
                     /locus_tag="ACG8ER_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS5049525.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14303..14659)
                     /locus_tag="ACG8ER_000926"
     CDS             complement(14303..14659)
                     /locus_tag="ACG8ER_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5049526.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15027..16214
                     /gene="tuf"
                     /locus_tag="ACG8ER_000927"
     CDS             15027..16214
                     /gene="tuf"
                     /locus_tag="ACG8ER_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5049527.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16402..18993)
                     /gene="mprF"
                     /locus_tag="ACG8ER_000928"
     CDS             complement(16402..18993)
                     /gene="mprF"
                     /locus_tag="ACG8ER_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5049528.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19019..19780)
                     /locus_tag="ACG8ER_000929"
     CDS             complement(19019..19780)
                     /locus_tag="ACG8ER_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5049529.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            19947..20579
                     /locus_tag="ACG8ER_000930"
     CDS             19947..20579
                     /locus_tag="ACG8ER_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5049530.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            20638..21498
                     /locus_tag="ACG8ER_000931"
     CDS             20638..21498
                     /locus_tag="ACG8ER_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS5049531.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            21683..23998
                     /locus_tag="ACG8ER_000932"
     CDS             21683..23998
                     /locus_tag="ACG8ER_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5049532.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24180..26021
                     /gene="dnaG"
                     /locus_tag="ACG8ER_000933"
     CDS             24180..26021
                     /gene="dnaG"
                     /locus_tag="ACG8ER_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS5049533.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26045..27154
                     /gene="rpoD"
                     /locus_tag="ACG8ER_000934"
     CDS             26045..27154
                     /gene="rpoD"
                     /locus_tag="ACG8ER_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS5049534.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            27371..29524
                     /locus_tag="ACG8ER_000935"
     CDS             27371..29524
                     /locus_tag="ACG8ER_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS5049535.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDTEKDISGVNAAGKESLKTNDLK
                     TNDLKTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAET
                     QSASEDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKK
                     SKKVPYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDH
                     HQFIRADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAI
                     VDDHLADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKD
                     GKVVDSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEE
                     AKKAEKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKS
                     ARASDIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYAT
                     STKSALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            29720..30583
                     /locus_tag="ACG8ER_000936"
     CDS             29720..30583
                     /locus_tag="ACG8ER_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS5049536.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            30688..31161
                     /locus_tag="ACG8ER_000937"
     CDS             30688..31161
                     /locus_tag="ACG8ER_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5049537.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            31257..32144
                     /gene="xerD"
                     /locus_tag="ACG8ER_000938"
     CDS             31257..32144
                     /gene="xerD"
                     /locus_tag="ACG8ER_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS5049538.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            32163..32534
                     /locus_tag="ACG8ER_000939"
     CDS             32163..32534
                     /locus_tag="ACG8ER_000939"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5049539.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            32531..33301
                     /locus_tag="ACG8ER_000940"
     CDS             32531..33301
                     /locus_tag="ACG8ER_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS5049540.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            33285..33854
                     /gene="scpB"
                     /locus_tag="ACG8ER_000941"
     CDS             33285..33854
                     /gene="scpB"
                     /locus_tag="ACG8ER_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS5049541.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            33860..34576
                     /locus_tag="ACG8ER_000942"
     CDS             33860..34576
                     /locus_tag="ACG8ER_000942"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5049542.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      34658..34782
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            34905..35516
                     /locus_tag="ACG8ER_000943"
     CDS             34905..35516
                     /locus_tag="ACG8ER_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5049543.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            35894..37417
                     /locus_tag="ACG8ER_000944"
     CDS             35894..37417
                     /locus_tag="ACG8ER_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5049544.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            37654..38994
                     /locus_tag="ACG8ER_000945"
     CDS             37654..38994
                     /locus_tag="ACG8ER_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5049545.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            39148..39885
                     /locus_tag="ACG8ER_000946"
     CDS             39148..39885
                     /locus_tag="ACG8ER_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5049546.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(40050..40271)
                     /locus_tag="ACG8ER_000947"
     CDS             complement(40050..40271)
                     /locus_tag="ACG8ER_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS5049547.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            40302..41333
                     /locus_tag="ACG8ER_000948"
     CDS             40302..41333
                     /locus_tag="ACG8ER_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049548.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            41320..42723
                     /locus_tag="ACG8ER_000949"
     CDS             41320..42723
                     /locus_tag="ACG8ER_000949"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS5049549.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            42789..43424
                     /locus_tag="ACG8ER_000950"
     CDS             42789..43424
                     /locus_tag="ACG8ER_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS5049550.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            43472..44152
                     /gene="cmk"
                     /locus_tag="ACG8ER_000951"
     CDS             43472..44152
                     /gene="cmk"
                     /locus_tag="ACG8ER_000951"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS5049551.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            44287..>45515
                     /gene="rpsA"
                     /locus_tag="ACG8ER_000952"
     CDS             44287..>45515
                     /gene="rpsA"
                     /locus_tag="ACG8ER_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS5049552.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT        15177 a         7930 c         9603 g        12805 t
ORIGIN      
        1 gacaactatt ttctgattta ttacaaatgt tttttcaaat aaatttgatt accaagaaaa
       61 ggtagtggta tatttactga gggatacttt gaaaaacata tgatagaaac agtaacagca
      121 ttatgaaaaa tctttttata aaaatggagg aatcgatttg atggaagatc agtatgtaca
      181 agcaatgacg atcgcaggat cagatagcga tggaagcgca gggatgcaag cagatatgca
      241 tacattcttt acaagaaatg tttacggtgt ttctgtcatg accgcatgtg ttgcaggtaa
      301 ttcttacgga atcggagcaa gtgtgacatt gccaacagat tttatcgaca aagaatttga
      361 actgattgcg aaggactttc aaatacgtgc agctaaaaca ggtatgctag cagattcaaa
      421 gttgattgag acagttgtaa aaaattacca aaaatatgat tttggaccat tagtcgtgga
      481 tcccgtaatc gtaacgaaac atggaaatct tttactagaa gaatctgctt tgcaaagctt
      541 gaaagaaaag cttgtccctt tggccgaagt actgacacct aatttttatg aagcagaaaa
      601 gctgactgga cggacattag cagctgaaga agattttgtg caagcagcaa aagaactgca
      661 agatatgggt gcaaaaaacg ttatgatcaa aggacatcat gctaaagatg cgcagcaaga
      721 aacagtcaat gactacgtat tgctcgaatc aggaaaatca ttttggttaa atgcaccatt
      781 ttatcctacc gaccggatca acggaactgg cgattcgcta tctgcatgta ttgcagctga
      841 gttagcgaaa ggaaaaacag ttgaacaagc gatccgattg gcaaaagact atgtaaaccg
      901 tgcaattcgt gaagaaataa atgtagggca caaatatgga cccatcaatc attgggcagc
      961 tggtgaatta gagtaaaaga aagaggtcgt gagcctgccg tccagctccg agtcacaagg
     1021 aacaagttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt
     1081 tggcaggaga acaccgtttg ttcggaagaa gggactgtga caagattttt gtcacagtcc
     1141 ctttaatctt agtatatttt tctctacttg aataaaaaaa ttataggatt ggagaaaata
     1201 atcgagaaat ttgttgcttg aataatagcc agttagacat ttccttgact gtttcagttg
     1261 tcatggatga acaatgtgtc tcatcttctt tgaactgagt agcaagttct tctaaaaact
     1321 caggatcgta aatgacagca cttgtttcaa aattcaatcg gtagctacga atatcttgat
     1381 ttgctgaacc taccatgcag atttcatcat ccattatcaa tgtttttgca tggaggaagc
     1441 cgttctcata gacaaggatt tgtacatttt ccttgatcag ctgccgagca taatattgtg
     1501 ttgctcgata aataaaagga tgatccggtt tgttgggaat catgattttc acatctacac
     1561 cagaggcagt tgcgattttt aaagcagcaa tgacactttc atcaggaaca aggtaaggtg
     1621 tctgtatcca aacacgtttt ttagcagaag taatcaattt gataaatgaa agcttgattt
     1681 gttccctttc gttattcggt ccgcttgaaa caagctggat ggatgtatca gcaagcttag
     1741 attcatctcg atctgctttt ttaaagaaat aattcaaatg atagccgacc tttttttctt
     1801 caggtaccga gacgttccag tctgttaaga agcgaagctg gagcaaagaa gaagcggcac
     1861 cgaaaatccg tatatgcgta tcgcgccaat agccgaattt ttttgttgta tttacatatt
     1921 gattggcaat attgaaacca ccggtataac taatctttcc atcgatcaca acgatttttc
     1981 ggtgatcatg gtaattcaat cggaaacgaa gtaatgcctt ttgtgaggta acaaatgtat
     2041 ggacatgtcc accatttttg attagacgat tgaaatcttt tgcttttgta ccatgagagc
     2101 caaatgcatc atataatatt cgaacttcca cgccttctgc ggctttttct tctaaaacat
     2161 gtaagacttg ctggccgata ttatctgtca caaatgcgta atattcgata tggatcgaat
     2221 gtttggcttt ttttagatct tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa
     2281 gagtgacaga attcattctt gtcagcggca tacgatttaa tgaagagaag aagtcgacaa
     2341 attgctgttt gtctttatcg cccatgtcag gatcgtaatg ttcaatggta tctcctctaa
     2401 aagactgaaa gttttctaat tctttcaagt cactttgtcg gagatagaat tttttcttat
     2461 ccgttaatcc acgtccgaaa aataaatata atacaaagcc caccccagga agggcaaaca
     2521 atactaagag ccatgcccaa atggctgcta catctcgggg tttgatcaaa atagccacca
     2581 aagcaataag tgcatttaat agataaaggg cggttataat actagatacc acaaaaatgt
     2641 tcctccttac atacaataac attagtatag catgatagaa gaaagaagcc taccaaggtg
     2701 aatatacttt tcactccttt catgaaaatg taggtaaatc gggctaaagt agttgctttt
     2761 tgtaggttaa taaggtagga tattagtaca gttgaatatc tccttagggg agtaaccggc
     2821 aacgtttgtt gcgataatat caacagtaag ttaaccaaat ggttttctgg tattgtatga
     2881 aatggtgaga cctaagacaa atgcgtattt gtcttaggtc tctttttttt tcagacagga
     2941 ctagggccaa gatcaaaaaa tgggggatga aatttggaaa acaaggaaca agcacaacaa
     3001 gcggtctata aagaaacgat cgactcgttg atgcagcaaa cgcagagctc aaccgatgga
     3061 ttgagcacca cgcaagcgaa ggaaaaatta gaaagagatg gcttgaacca gctaaaagaa
     3121 aagccgaaga agccaacttg gaaattgttc ttagaaactt tcaaagatgc aatggttatc
     3181 gtattgctga ttgtcgcact gattcagatg gtcatgggtg actacatcga attcttagtt
     3241 atttttgcag tattgatgtt gaattctata atcagtgttg tccaaacaaa aaaagcagaa
     3301 ggttctttag aagcgttgaa aaacctttca gcgcccgatg ctcgagtgat tcgtgatggt
     3361 aaggaaaaaa cgattccggc aaacgaactt gtggtaggag atatcgtgtt gttagacgca
     3421 ggcgattatg tacctgctga tggacgttta ctagatgcgg gatcattaaa agtagatgaa
     3481 ggtatgctga caggagagtc gacttctgca gaaaaagaag taacggatat cacaagtacg
     3541 gtaccaatcg gagatcgtac aaatatggta ttcagtggaa cgatcgtaac ttacggacgc
     3601 ggtaaattct tagtgacttc aactgggaat aatacggaga tcggacaagt tgctggcctt
     3661 ttggaatcaa caactgagca agtcactccg ttacaacgag gtctggataa attcagtaaa
     3721 aaactaagtc tggctatttt ggcattgtct ttattgattt taggtatcca actgtttcgt
     3781 atttatcttg gagaaggtac aggagatatg actgcagata tcgtcagcgc agtgatgttt
     3841 gccgtagccg tagccgtagc tgctattcca gaagcattac agtctatcgt aacaatcgtg
     3901 ttatctttgg gaacgaataa aatggcaaaa cgtcatgcca ttattcggaa gcttccagca
     3961 gttgaaacac ttggttcgac aagtatcatc tgtacagata agactggtac attgacacaa
     4021 aacaaaatga cagttgttga ctacttttta ggtaatggcg atactgggga tttcacaaat
     4081 gacccttcaa aatggacagc tgacgaacgt cgcttgatcg aaatcagtgt tttagcgaat
     4141 gatgcatcga tcagtgaaga cggtactaaa ttgggcgacc ctacggaagt agcgtttatc
     4201 gactttagtg aaaaattaaa ccagccttat caggaaatcc gaaacaacta cccaagacaa
     4261 gcagaacttc ctttcgattc tgatcgaaaa ttaatgtcta ctggacacac gatcgataac
     4321 gaaaattact tgtttgtcaa aggcggacca gttatcgtct tccaacgcag taaaaaagta
     4381 ttgctaaatg gtgaagtcgt tccgatgacg gatgaattga tgaaaaaatt ccaagagcaa
     4441 aatgaagctt tttcaacacg cgcattacgt gtactagctt ttgcttaccg tccacttgat
     4501 aattttgatt tatcatttga agatgaagac gatttgatcc ttgttggttt gcttgctatg
     4561 atcgatcctc cacgtgctga agtgactcag gcggtaacag aagcaagaag tgcaggaatc
     4621 aaaacaatca tgattactgg tgaccacaaa acaacagctg ttgctatcgc aaaacaaatc
     4681 gggatttttg aagaaggaaa cctggctttg accgggacgg aactagatga attgacagaa
     4741 gatcaactaa acgaagtttt agaaaaagtc actgtttatg cacgtgtttc acctgagaat
     4801 aaaattcgta tcgttcgtgc ttggcaaaat aaaggtcata tctcagctat gacaggtgat
     4861 ggtgtaaatg acgcaccagc attgaaacaa gcagacatcg gaattgctat gggaacgggt
     4921 acagatgtgg caaaagatgc agcagcgatg gtattgacgg atgataactt tgcttctatt
     4981 gtcagcgcag tagaagtagg acgtaatgtg tttgataata tcaagaaagc tatttcttat
     5041 ctatttgcag gaaatttagg tgcgatcatt gcgatcatct ttgccttagt tgctgactgg
     5101 tccaatccat ttacagcatt acaactgttg ttcatcaatt tagtcaatga ttcactgcca
     5161 gcaattgcat taggtacaga aaaagctgaa cctgctacga tgaaccgccc gccaagagat
     5221 ccaaattcag gaatctttac ggacaaaaca ttgatttctg taacgtatcg tggtgtgctg
     5281 attgggattg ctgttatctt tgctcaatgg cttggcatgc agcattcacc tgaattcggt
     5341 gtagccatgt cattttcaac attgatttgg tgtcggacat tgcaaacatt cccagcacgt
     5401 tcaaacacac aaacagctat ccaagcaggg ttcttctcta acagaagtgt cttactagct
     5461 gtagttgtat gtagcgcgct ttactgctta acaatgatcc caggactgaa agaagtcttc
     5521 tctatcccat cagcatttgg attgacagaa gtctttacat gtatgggaat cgcacttgtg
     5581 acaattgttt tgatggaaat cactaaattg atcattgtac gagtacaagg aaataaataa
     5641 aacgaatcaa cgaggttgtg agcctgccgt ttaattctga gtcacaaggt gcaatttcaa
     5701 caaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaagagtt ggcaggagaa
     5761 caccgtttaa ggaagaagtt gtaaaatcag cattttgagc taatactgag ggtcatctga
     5821 ttggacactg tttattcgga aagagggact ttgacacgac tttggtcaca gtcccttttt
     5881 ttatttatga tagacggaaa gccaatcatt ttattattca tcaaaagaga tagaatagat
     5941 aaaagaggag aataatttat tccgaaaagt gaaaacatat aacaaaaaat tagaggaaat
     6001 caaatgaatg aaaaagaatg ggatgaattt gctgaagaat attatgaaat ccagcaagaa
     6061 tcgcaagtaa ctattgctga agatgtaaaa aattatctga aaacggagaa agtcctgcct
     6121 gctgaaacag cagcagacgt tgctggagga tctggcagat ttctttcctt agcaaaagaa
     6181 attaatcgct atgatttgat cgatttttct tcaggaatgc tagaaaatgc aaaaaaagaa
     6241 gcagaaaaaa atgagttgcc aaatgtttcc tttatcaaac agtcgttaga agaatttatc
     6301 caatcaaatg agacatatga aatggttttt acagcagcaa atccagcatt ggtacatctt
     6361 attcaattag aaaaattgat gaaaaagtca agcaaatggt gcgtgataat cagggtagtg
     6421 gaatcaatcg atgatgtttt ttatcctatc gaaaagcaac taggaatgat agaagaagat
     6481 cctaatacat cgccagttgt aatggatcaa tttgaaacat acttaattga tgaaggattc
     6541 aaagtgaaga aaaaagattt tacttatttt acacaggaat caattactga agaattgatg
     6601 aagctatact atgacgcgta cgcaaaagat cctgttgtga ccaggtacat tcgcaaacta
     6661 ttcagtgaaa aacgagaaat ccttagtacg actaagctga cctatagact gctcctttgg
     6721 tccgcagata aaaaaagaac ctttcacgac tcgtgaaggg ttctttttat gattttatta
     6781 tattttgtag taacgaatat cattttcgat ctttgtaatc gaaaaaggta catctgaagt
     6841 atattctgca cattctccgt gttcaggaag cgtctcactg aaaatcgttt catattcgtc
     6901 aacagtgatc cgtgttcgag aatctagcat ctcttgatga gattgtgcga agagatattc
     6961 ttgatagttt tcttccagat aacctgtaaa aaattcactt actgcaccag aaccgtagct
     7021 gaatagaccg atgcgatctc ctggctgtag tgatttcgaa ttttccagta gagaagttag
     7081 ccccaggtat aatgaaccag tgtaaagatt accgattcgt cggctgtaac ggatgctttc
     7141 ttcatagcga gccataagac gttcctgatt ttcttcgtct gtttggtcta atacgctttg
     7201 caatgccttt tttcccatct tagtatacgg aatatggaaa gcaatcgcct gataatcttc
     7261 gagtcctcga cccgacaatt ctttatgtcg attccaaact ttttggaatg attcgatata
     7321 cgtagaatta gataaaggac catcaacaac aggaaattcg ctataatctg gacgccagaa
     7381 atcatagata tcttctgtca gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt
     7441 ttgagtgatc atcatcgcaa cagcaccgac accttgcgtc acttcaccac cgcttgccaa
     7501 gccgtaacga gcaatatcac ttgctatgac tagtactttt cgttctggat gatttttgac
     7561 atattctttc gccatgtgca aagctgcggt gccaccgtag caagcctctt ttacttcaaa
     7621 agaacgagca aaagattgga ttttcagtaa atggtggata attaccgcac tcgcttttga
     7681 atgatcaatc ccggattctg ttccgacgat gaccatgtcg attagctcac ggtctttttc
     7741 tgttacgatt ttgctggcag catttgctcc tagtgtaatg atgtcttcat ttgcacgatt
     7801 gactgccatt tggtcttgac caatccctat atggtatttt gcaggatcat ccccacggct
     7861 ttctgccaac tctgtcatat ctaaatataa attaggaata aaaaaggaaa gacgatcaat
     7921 ccctattttc atttcaacac ctctttactt atgataaaaa acttacttta ctaaggtatc
     7981 ataaaaagat taaaagaaat ataaaaaaga gaatatttaa ggattgttaa aatttaatct
     8041 tagaaaaaaa caactggaac aagagctatt tgtttttaca gcaaagaatg gtatattttt
     8101 gtaggaggtg gcttacttga aagaagtcgt tatgatagat gctgcaagaa cgccgatagg
     8161 aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa ttaggcacac ttgtgactaa
     8221 aggattatta gataaaacaa aactgaaaaa agataaaatc gatcaagtaa tattcggaaa
     8281 tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag attgctttga acagcggtct
     8341 ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt
     8401 gatcttagca cgccagctga tccaattagg tgaagcagaa cttgtgatag ccggcggaac
     8461 agagagtatg tctcaagcac ctatgctgaa accgtatcag tcagaaacaa atgaatatgg
     8521 tgaaccaatt tccagtatgg tcaacgacgg attgactgac gcattttcaa atgcacatat
     8581 gggattaacc gcagagaagg ttgcaacaca attttctgtg agcagagaag aacaggatcg
     8641 ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc gaagccggtg tattttctga
     8701 ggagatcatc ccagtcaaga tttctgatga agacgtgtta tctgaggatg aagcagttcg
     8761 tggaaatagt acattggaaa aactgggcac gttacgtaca gtattctcag aagaaggaac
     8821 tgtaacagca ggaaatgctt cgccattgaa tgacggtgcc tctgtggtga tccttgcatc
     8881 caaagaatac gcagaaaata ataatctgcc ttatttagca accatcaaag aagtagcgga
     8941 ggtcggtatt gacccaagta tcatgggaat cgctccaatc aaagccattc aaaaattgac
     9001 agatagatca ggaatgaatt tatcaacaat cgatcttttt gagatcaatg aagcatttgc
     9061 agcttcttct atcgtagtta gccaagaact gcagttagat gaagaaaagg tcaatatata
     9121 tggaggagca atcgcattag gccatccaat tggtgcaagc ggtgcacgta tcttgactac
     9181 attagcttac ggattgcttc gtgaacagaa gagatacggt attgcttcat tatgtattgg
     9241 tggaggttta ggcttggcag tcttgttaga agcgaatatg gagcaaacac ataaggatgt
     9301 gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg cgcagtcaat taatagaaaa
     9361 aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa caaacacttt ctgaagagct
     9421 ctccgatcac atgatcgaaa accaagtgag tgaagtggaa atcccaatgg gcatcgcgca
     9481 gaattttcaa atcaatggta agaaaaaatg gattccgatg gcaacagagg aaccatctgt
     9541 cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca
     9601 gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa
     9661 tgcagttaat catcgaaaag aagagctgat tctttgtgca aatgaaagtt atccttcaat
     9721 cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg gaattcatgg ggagtttcca
     9781 tgcatactta tctattgatt ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat
     9841 caactcgatt cttgagagtg tggcaaacaa gttacgggag tggttccctg aagaagagat
     9901 tctattcagt attttaagta attttgctac agaatcattg gcttctgcct gttgtgaaat
     9961 cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca
    10021 acaggcaggg gaatatgcga agcttgaccc ttaccgtgca gcaacgcaca acaaagggat
    10081 catgaatggt atagaagctg tagtggcagc tacaggaaat gatacacgtg cggttagcgc
    10141 ttcgattcac gcctacgcgg caagaaatgg attataccaa ggattaacag actggcagat
    10201 caaaggggat aaactagttg gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg
    10261 agcttccaat atcctgccaa aagcaaaagc atctttagct atgttagata ttgattcagc
    10321 caaggaacta gcacaagtaa ttgctgcagt agggttagct caaaacctgg ctgctttgcg
    10381 tgcattagtt acagaaggaa tccaaaaagg ccacatggga cttcaagctc gttcattagc
    10441 gatctcaatt ggggctattg gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc
    10501 agaaaagatg aaccagcaga cagctataca aatattggaa aaaatccggg aaaaataaaa
    10561 aagcaatatc tttagaagaa taaagcaaga tttacagtta ttttatttac tgcaaatctt
    10621 gcttttttct ctatcagaaa atatgtttat tattagaatt ttttatagtt aaggttaggc
    10681 atcttaagaa atcaggcaac tccaagatat cttcaggtag aagagaagtc gcctaacatt
    10741 gtaggatcat aggtgatttg ttggacaggt caagctgata tttgagcctg atgttagaag
    10801 gaatttcttt ttacttgctg tagaaattat tatatgacta aaaaggctgg gattttaaat
    10861 cccagccttt tatgacaaat agaattttta tcgatcagta atcgctgtcg ctgcaaattg
    10921 ttcgatcaaa ggaatcaaag tcaatttgac tttgtatttc cgttcgagtg ctttttcgat
    10981 aacgaactca ttttcagtag tcatagaaat agttgctaat ttacgaggtt catcagcatt
    11041 tttttcatta ataactactt catgttcatt ttgttctgtg taaaatttag gatactcagc
    11101 aacggctttt gaaaaatcag caaaacttaa agcggcattc gtatcagttg agcgtgtatt
    11161 ttcagttgtg tccgcttttg gtttttccgt tttttcttct ggcacactga ctgtttcttc
    11221 tgtaactggt gtcgcaacag cttgcgcagt tggttcatcg tttttcaatc gttgatcatt
    11281 ccagtaagac acgatttcaa taagaagatt gtcacggaat cgttcgaaaa tcccgttttc
    11341 tcctgaacga attctcttta atacagtatt gaagttgtca tcgattggca cgttgatccg
    11401 tgtatcttca tttgttttcc cagctaatcc gatcgttaag atttgacggt ttttgggatc
    11461 aagcatgact tgtaacggca aaccatagcg tcgcaagctc tcttcttcta gagaacggaa
    11521 taattgtcct cttacaccat ccaatgaagg acggaattga tttaataatt tttgtttctg
    11581 ttttttcttc atatgttctc tccttcgctc ataccctttc gattataaga aaaaaacagc
    11641 caaatgaaaa ggagtaatgc aaaaaaactt gcgtatacgc aaattacaac agaaaaattt
    11701 gcgtattttc ccgttttttg aaagaaaaat aaaaaatgga acggattatg tatatataca
    11761 taatccgttc cattctttct attttttcgg tatcaaggca aaaacaggta gttcaggtga
    11821 ataaatgact tcatgttcgt gccagggttt gaccattagc gaatgatcga accgtttttt
    11881 cagttcttcg atgatttgat catgtgtcca gtttcttgca ggaaaatgtt ggattttctc
    11941 tgcatcgatc aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt ccgagagaga
    12001 attcaagcgt tcgattggat ccataaaatc acctttcgac ttaaaattgg gtagtatctg
    12061 ggtcttttgc tttgccgact acaccatcta actgatcgat ttgtttcatt tcttcttctg
    12121 ttaattcaaa atcaaataat tcagtatttt ctttgattct gcttggtgtc actgacttag
    12181 gaagaggaag gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt
    12241 catgtgcttc agcgatttgt ttcatttcag gcacatcgaa gattttacca gtgcctaatg
    12301 gactatatgc ttcgaggatc atatcatgtt tttttgcata ctcaactaca gttggctgta
    12361 attcgcctgg tgcaaggaaa acttgattga ccatcggcat aatttttgct gtttttgcta
    12421 gtgtatctag atggtggggt aagaaattgc tcacaccgat tgctttgatt ttacctgctt
    12481 catacagttc ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa
    12541 aagcaacagg gtttggccag tggatcaaaa acagatctaa ataatctgtt tggagcttct
    12601 tcaatgattc ttcaaaagaa gacatcacaa gttcatagct gtggtttgca ttccataatt
    12661 ttgttgtcaa gaatatctct tctctaggaa ttccactttc cttaatcgct tggccgacac
    12721 tttcttcatt cttgtatcct tgggcagtat cgatatggcg ataacctgca gccagcgctt
    12781 ctttaacaga ggaaacagcg acatccccat caggtgtttg ccatgtgccg aatcctacta
    12841 ccgggatttc gtaaccattt aatagacgat aagtgcttgt taatgaattc atactttcag
    12901 ctcctttttt attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt
    12961 tgtttctact cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat
    13021 tttttttccg taaactaaga cagaagggaa tgagaaaatg gatatctatt taaaaaaagc
    13081 aattctgcat attattgaca gagaaactgg ttctccagtc ttttcacaaa aagagctgga
    13141 tctgacaaaa gaatatattc gtgattttct tcaaaagaag atccaaaaaa tctcttctgc
    13201 tcaaacaaaa actggacaac tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc
    13261 tcaagaaaat tttgtggaag caagtgaagc aatcgttcag cggtggttcg atatctacca
    13321 agaaagtgaa gaagcaccta gtgcagatgt atttgttgtt ttgtatgaat tagatacagt
    13381 gatgtactta tctttattga aagtcaatta tcgtgaggct tacactcact ttgttgaagc
    13441 agacgaagca gggatcgaca accgcttgat tttgaatcga gcaattctag gggcgaaaac
    13501 acaaaaagca gatgaagcat tcgctgtgaa tttgactgat tggacctacg aattaattga
    13561 aaaaaaatat gagttttctg gcaaaaaaga gctttatttt tctggaagag tgatcgaaag
    13621 tgttcctgca ccttctttag aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct
    13681 tggaaaaaaa tttgagacag aagaatttga tatcatggct gatttgaaag aagctgtcta
    13741 tgatacgata gaagaaaaag gtcggttgga tcatgagatg attgcggaaa aagtcttcaa
    13801 ggaaaacatc acggcaaaaa tggcttttaa agaagaagta caagagcaag gatttgtccc
    13861 agaagcacct cctgtaaaag aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa
    13921 attcaagcta tcaaacggga tcgaactgat cgtaccagta gatgtatatc gcaacccaga
    13981 tttgattgaa tttgtgaata atccggatgg tacgatatcc gtgatgatca aaaatgtgga
    14041 tgaagtattg aatcgtttat aaaaatgact agaacattag gtgttcaagt accagtgatc
    14101 agttaaatga attgtgtcaa gagaaggatc ggaaaagacg aatataacaa aaaaagagcg
    14161 tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
    14221 agaagcaact ccttcttatt tcttggagtt aaacgcttct gtcccagcct ctttttatct
    14281 aataaaattt taagaccatt tactaattat cgattttttc tgccgcattt ttaggcactt
    14341 cagaagcctt tatttcttcc cagcttaata cacctttacg aggattgact ttgagtttaa
    14401 gatacgcatt catctttaat ggatgttctc tttcttcgcg aagcgtcacc tgtttttttt
    14461 caccatcttt attataagcc gcttgcttgt aggtataagt cgtgaatttc tctccgttat
    14521 ctgcttgggt agctgacatt tcgccattcg tcgtgatttt tgtataataa gcttcgccgc
    14581 cgtaaaaata atgataagct gcaaaacttc cgccgagaaa tatgcctaaa acgacaagta
    14641 cgccaattaa ttttttcatg tacttcacct ctttccttat tattaacgaa atagaagatt
    14701 tcgtctattg aaagttgttg gcaacagctg aattttaaac ttttcctcat tttgttctta
    14761 ttgcttcaaa agaaagttaa ggactatctt gaagtttttc tcaaaaaaca cattttttta
    14821 tttataaaag gtactttttt cgcgtataag acaagcgagt gctcttagtt gactttgacc
    14881 aatattgacg tataataagc ttagttcgaa tcttacaaaa agtaagtcgg acgtattctt
    14941 tttgatttag tcaaaaaaag actataataa aaaggtgaga gctgaaattt ttcagtgatc
    15001 tatattattt aggaggtttg taaattatgg caaaagaaca ttatgacaga agtaaaccac
    15061 acgttaacat tggtacgatt ggacacgttg accatgggaa aactacttta actgctgcga
    15121 tcactacagt attaggcaaa aaaggcctag caaatcctca agactatgct agtattgatg
    15181 cagctccaga agaacgtgaa cgtgggatta cgatcaatac cgcacacgta gaatatgaaa
    15241 cagaaaaacg ccactacgcg catattgatg caccaggaca cgcggactat gtgaaaaaca
    15301 tgattacagg ggcagcacaa atggatggtg caatcttagt tgtttctgca actgacggtc
    15361 cgatgcctca aacacgtgaa cacattttat tgtcacgcca agttggtgta aaatacctga
    15421 ttgttttctt gaacaaagtt gatttagtcg atgatgaaga attgatcgat ttggtagaaa
    15481 tggaagttcg cgagttattg agcgaatatg gtttcccagg cgatgacact cctgtgatca
    15541 aaggttccgc attaaaagca ttgcaaggcg atccagatgc tgaagctgct attatggaat
    15601 tgatggatac agtagatgaa tatatcccaa caccagaacg tgatacagat aaaccattac
    15661 tattgccagt ggaagacgtc ttctcaatta caggtcgagg aactgttgcc tcaggtcgta
    15721 ttgatcgtgg tgctgttcgt gtcggtgatg aggtagagat cgtagggatc aaacctgaaa
    15781 cacaaaaagc agttgtaaca ggtgtagaaa tgttccgtaa aacgttagat tacggggaag
    15841 ctggggataa cgtaggcgtg ttgttacggg ggatccaacg tgacgatatc gaacgtggac
    15901 aagtgcttgc taaaccaggt tccattactc cacatacaaa attcaaagca gaagtgtacg
    15961 tgttgacaaa agaagaaggt ggacgtcata ctccattctt caacaactat cgtccacaat
    16021 tctacttccg cacaactgat gttacaggaa caatcacatt gccagaagat acagaaatgg
    16081 tcatgcctgg tgacaacgtg acgattgatg ttgacttgat ccatccaatc gctgttgaaa
    16141 acggaacgac attctcaatt cgtgaaggcg gacggacagt gggttcaggt atcgttacag
    16201 agatcgaagc ataagaaacg cgactcgaat attttggtaa tgaaaaatgt ttaaaataaa
    16261 agagcggtcc aaacaggatt tctgtttgga ccgctctttg ttcttcaatt gtataagaag
    16321 agattgtaga agcatgaact ttcgttttac ttcagtctct tttattcatc atgagagtgt
    16381 tcatctatcg gtatgcagtt tttaatactt tcttcgtatc cattttttca aaccacttat
    16441 tctaccttgt gtctgctcaa ctggcgcatt atcaataatc agcaaagcaa tcataacata
    16501 tgcaatccaa ctatctctag aatatagagt ataacgtgag acccagtttg tagcgtactt
    16561 gcttttatat tctcttaatc catggaaaga ataaaaatga gaaccaaact cgtaaaccaa
    16621 gtatgcaatc ctttcctgta aaaaactctt tctggaagtt ccgacatttg aaagaggggc
    16681 catccctaaa ttgaaccatt ggatatgttc ttctttcata taggcaaaca aatgcaagaa
    16741 caagaaatcc atactgccag aaggtgcttt tttaggatca tatcgcatca gatcaatcgt
    16801 tcctacttta ttgttcgtgt aagtaggcat gatcgttgca aaagcgacaa tttcttcttg
    16861 ggtatttttg acaactgcaa taggcgctct ttgtagataa tcttcagaga aaaagcctaa
    16921 agaaaaccct ttttcctttc gtgaacccaa ccaattatca gagatatttt tgaaagtact
    16981 catttgctca gtagaaaatg gtggttgtaa gacatcaaat gtaaatcctt ctcgttcgat
    17041 ccgattcaag acagcacgag tgcccttcat ttttttccct gaagttgtaa aactattcag
    17101 gtctacataa gcttcttccc ccatcttaat gaaatcgtag ccgaattcat gcaaaatcat
    17161 aacaatctct tcgcttgttt cgtagaaaac tggtaaatag catagtcgat ctgtttcttc
    17221 gataaaagct tcgattgctt ctggaaaatc ttctttctta ccagaaggat cacccattac
    17281 aatacattta ttattgaagc aagcgaattg gagaagaaca gttggttcac cgtctttttc
    17341 ataggcaaac atccgcttat cttttaagaa gatcaattga ctatcggagt ttcctccata
    17401 agttgtaagg atcttcaggg ccttttcttc attaaaggct tcgccaatct gtttcttttc
    17461 tccttgaagg aaatgaacaa atagaacgat catgaaacta acagcgatga tggccaacaa
    17521 tcccgaaaac caaatctttt cagaaggaaa taagaaaaag gaaataaaat ggtgacgccg
    17581 atgagggaaa tccggcagat tgtatacccc aataacgatg taaagcaagg acagtgcacc
    17641 aataatgatt ccatcgatcg tacgccactc ccatgaatag atcaattgtt cccgaaataa
    17701 ttcgttttta gaagcaagaa tgatcagaag cagaatagac aagaaaacca ccggtgtgaa
    17761 actaaaatca cttaataaaa cataaagtaa agctagaaca atcaagaaga ttgtaggcaa
    17821 atatgctcgt ttcacacgtg ctgcgatccc tctgcccata atgattaaaa gaaatcccaa
    17881 tagtatacta ggaaattgga taataaaatg aaatttcaat gggttcaaac tatgaagcca
    17941 gcgaaactct gtaaaagctt gcgggatagt ggcagaaagt actagcatga ttccagaaaa
    18001 gtataataag acaaccacga ttttatgcgc aatttctgtt gctaattgtt tagggatacc
    18061 agaataacgc tgatcaaaag tggaaccgat atttttgaag aaaaagacga ttccaatcaa
    18121 aaacggaatg atgtagtaga acaggcgata gagcaagatc cacacaacga ctatttctct
    18181 tggcacacca atagcggaca acccgataat catcattaca tcgaaactcc caatttctcc
    18241 tggaatcata gacacgattc ctatcactga agcagcaatg aataacggaa ttgtttgcca
    18301 cagatcaatc cggatttcca ttaaataacc aacagaaagg aaactaaaca ggacaccggt
    18361 ccattccaac aatgaaacta gcaatagctg tccttgagtt ttaagtgagg taccaccaag
    18421 gaatcctttc tttttgaaag tagtaaataa aaagacgaca ggaaaatata gaccgccgcc
    18481 gatcaaccat atccaatatt gcttcaaaaa aggagcaact ggcgtgaaga ctacaagtaa
    18541 aaaagaaagc agactgtaaa tagaaagtcc agccatcaaa aacaagaaaa ctttcgataa
    18601 tgcttggatc acgtttctgc tctctttctt ttgtccatat agttcagagc gaagccccac
    18661 actaacaaac ccgccgaaac cggcaatatt gttgattgta ttgatcagcc agcttgtctc
    18721 aaacaagtaa cgaggattct gcttttgttc aagcatccga ttcaaaataa tatcataacc
    18781 aatcataggt aacacagaga caagaccaat caacagcata agtccgcttt tccatacagg
    18841 tatagtggca aacgtttctc ctaattgctg gatagataga gttttaccga tggacatcaa
    18901 ttcaccgaca ataatgacaa tgacagaaat caaaaagatt gttttgaaca gtcctaggtg
    18961 gtttttcagc cattgtatgg tattttttaa caaataaatc accttctatt cctaatagtt
    19021 atgtttcaga aagaaattga agaacttctc gattgaagcg ttcaggatct tttctagcta
    19081 attcatgtcc ttgttctttc acaagcacaa aggaagcttt tggtatagtt ttagctatat
    19141 acaaagaatg ttttagcttg attacatctt tttttcccac gatgatcaaa gtgggggagt
    19201 tgattttttt taaatcattt tctgtaagcc cgatgtcatg cagcaaaagt ttgataacta
    19261 aaagattttt gcgaagactt gggcgaaata aagagagtaa ccatacccat gcataatgaa
    19321 tattactgat gactctggca gaaaaacgaa cgcctttaac aagcgtgttt ccagaattta
    19381 aaatcaatcg atgaactttt ttagggaagc tgctggcaaa taccagtgct agatttgctc
    19441 catcactaaa tccaagaaaa tctgcctgat cgattttttc cagcaacatg atcgtattca
    19501 gatcctcggc cattaactga aaattcaaca tagatgcttc gtttgtcgaa cgaccatgtc
    19561 ctcggctatc tactagatac acggtatagt agcgttcgag tacagggacc tgttctgaaa
    19621 aaaagcggcc gctgccgtca ttgccatgca gtaaaaagag agggaagcct tgaccgctct
    19681 tttcatagta aatttcgctc ccatctggca tttttgcata tttcttttct tttttcacat
    19741 aatcacctac tgtgtaaagt ataacgatta tatcagtcat tgggcaattg tttttactga
    19801 aaaacaacga tcattttcgt aagatgacaa aaaatacagg aaaattcatt ttttcttaag
    19861 gcgtacacag aaatcaagga agaaatagcc aagaagataa agaaatagta agataaaaaa
    19921 agatgtcaaa ataactaggt gatgaaatga agcgaaaacg attatctatt atccgtattc
    19981 ttttttacat gatcattctg acggttattg gtgcggtgtt gtttttagga ggaagaactt
    20041 atcgttcggt aaaacgagtg gaaagctatc aaatagaaat aacaacagct gcaaaaaatt
    20101 atcatatgga aaactacgag aatttgatca aggcgataat attgaccgaa tcaaaaggaa
    20161 aagggacaga tctcatgcgg agctcggaaa gtctgcatgg agaagctgag atcattgata
    20221 atccagctga cagtattgat caaggtgtca aatatttggc ggaaatgata gtagaagcaa
    20281 agaaacaagg atgcgattta tggacagctg tccaagcata taattttggg aaagattata
    20341 ttgcctatgt aaaaaatcat ggcggagaaa acacagtaga attagcagaa gcgtattcta
    20401 aagatgtgct gagcccactt ttaggaaatg aggaagggaa acaataccga tattggcgga
    20461 tccagtcctt gctttacaat ggcggctatc tctatcataa tggaggtaac atgttttatc
    20521 ctgatattgt caaaatgaat gaaaaaataa tagagaccta tgaaaaagtt tttaagtaaa
    20581 atagcaggaa atgttcaatc atgaataaaa ttaaatgaaa gaaggagtgc gcaaatagtg
    20641 gaaaaaaggc aaaaaatgga aaatcaaaaa gtggggtaca caaccaaaga ctggatgtta
    20701 cggtttgtga agggaatgtt tatcggttca ggatttatct tgccaggagt cagcggtggt
    20761 gcattagcag ctgtctttgg catgtatgag cggctgattt cttttttagc ccaccttaca
    20821 aaaaatttca aagaaaatgt tttgtttttt ctgcctgttg gtctaggcgg agtgacaggc
    20881 atctttctac tttctttttt agtcagtttt ttattaggtg cgtatgaaac aacgatatta
    20941 tggttttttg tcggatgtat cgctggaact gttcctgcgt tatggaaaga agcagggaaa
    21001 aaaggaagag atacttctga tgtgatcatc atggttgtta cttttgttct ggcttatttg
    21061 tttttattat acggcgctca attatttgac gggcaagtcg atcagaattt tggaacatgg
    21121 ctgattgccg gcggattgat tggtttgggt atgatcgtcc caggattgag cccttctaat
    21181 tttttggtgt atatgggatt gtataaagcg atggctgacg gatttaaaga agtacgactt
    21241 gaagtgattg tgccgatcgg aattggtggg attctttgtg ttcttggatt gtcaaaaatc
    21301 atggattaca ttttctcaaa ggcatatagc aagctattcc atttcatttt aggtgtagtt
    21361 tttgcttcaa cgattatgat cattcctacc gattacgcgg gattgggagt tgctggggta
    21421 ctagcttgtc ctgttctttt tatagccggt acagcactag gttggtggat gagccgtttg
    21481 gaggaacaat ataagtaaag atagattgat aataaaaaca gcacacgggt gattcaaaca
    21541 aaaaggttgg catctttttt gtaaaatcat tcgtgtgttt ttctataaat tttataaaaa
    21601 caagttgttt ttaatcttgg aacacgctag acttaaacac gtgattattt ttgaaagttg
    21661 gcggaaaatg gagaaaaaga aaatgaatgg acttacacag caagaggtag ttcagaaaat
    21721 ggcttctggc cagcaaaatg actaccagga agatgcatca aaaagtacaa aacaaatatt
    21781 cagcgataat atattgacgt tattcaattt tctgaacttt ggtattggga tttgtctgtt
    21841 atttgtaggt gcgtattcca acatggcgta tttagcaatt attgtcgtca atatcgttat
    21901 tggaatttat caagaaatac atgcaagaaa tcttgtacgg aaattatcga ttgtttccca
    21961 atcagaagta ctagtgatcc gggacggtaa agaacaatcg atttctacta aagagctggt
    22021 attaggagat ttggtaaaaa tcggagcagg agaacagata ccagccgatt tacaggttat
    22081 atctggacgg gcagaagcaa atgaagctct tttgacaggc gaatctgatt tgattgtcaa
    22141 agaaactgga gctgaattgc tttctggaag ctttttgaca agcggttctg tcatggctga
    22201 agttatccga gtaggtgcag ataattatgc tgtccgcttg actagcgagg caaagactca
    22261 caaagctatt cgttctgaac ttgtagattc gattcgcaaa gtatccaaat tcactagttt
    22321 cgtgattatt cctttgggga tcatactatt tttggaagca ctgttcatcc gcgatgcttc
    22381 tatcaaaatt tctgttattg catctgcagc agctttgtta ggcatgcttc caaagggttt
    22441 ggtattgcta ataagtattg cattaacgac aggggtaacc aaactggcaa aaaaacgaat
    22501 ccttgttcaa gacatgtatt caattgaaac gctggcccat gtcgatacat tatgcttgga
    22561 taaaacaggg accattactg aaggaaaaat gagtatccag cgggtagaag cactacatcc
    22621 gctgtacgag aaagccatcc cagaaattat ggggacgtat ctggcagaaa gcagtgataa
    22681 caacatcact atgcgggcta tgagagatta ttttgagctt tctaatcggt atgatgtgac
    22741 aaacaaagtc gcattttctt ctgatcgcaa atggggcgcg atggaatttc cagaactggg
    22801 agtcgtgtac gtaggagcgc ctgaacgttt ggtggaagaa gatcggcttc cggacagtct
    22861 aaaaacagcc caagcgaatg gctatcgcgt acttatgtta gggatcgctc caaatcagca
    22921 attgaacgaa gaaaaaccag catttgttga gccggttgct ctgtttgaaa tcgatgatcc
    22981 aatccgaaaa aacgctcaag aaacactagc ttatcttcat gaggaaggtg tggatctgaa
    23041 agtgatttct ggagacaatc cttttactgt ctccaatatc gctagacgtg caggattacc
    23101 gaattatgat gcgtatgtcg atctttctca aattactgaa gaaatggaag tgcgagaagc
    23161 tgctcatcgc tatacggttt ttggacgagt gagtcctcag caaaagaaat tgctggtaaa
    23221 tgaattgaaa gaaagcggca gaacggttgc aatgacaggc gacggtgtga atgatgtttt
    23281 ggcgttaaga gaagcagatt gtagcattgc tatggcagaa ggtgatggag caacacgtca
    23341 gattgcgaat ctggtattgt tggactctga ttttactaca ttgccagaag tcctgtttga
    23401 aggacgccga gtggtcaaca atgtgacgaa agtctcaggc atctttttta tcaaaacgat
    23461 ttattcattt attttatcga ttatatgtgc cgttacagca attggatttc cgtttatccc
    23521 tatccaaatc acgctgattg atttagcgat tgaagggtat ccttccttct ttttatcttt
    23581 tgaacaagat aaaagaaaaa tcacttaccg ttatttgcct acagcactta ttaatgcttt
    23641 accaaatgcc ttgcttgtag tagtgaatat cattgcggtg tatctacttg gaaaaacatt
    23701 cgcatgggga aatgctgaaa ctactacttt gatgtattac ttgctgattg ggatcagttg
    23761 catggcagtt gtaaaagctt gttatccctt caacccattg cgcatcttcc tagcagtaac
    23821 gacgatcgta gggatatatg tagctgcaat gctgttccat ggaattttgg aagtaaactt
    23881 agggttggga gagagcgtag gatacttctt tgtattcatg ctcgtaaata ttgttcttcg
    23941 agtattgtac tggaaggctg atattcaaaa tcagttactg aaaagagtag caaattgatg
    24001 cttaatgaga tagaaatgtg ttatacttga aaaagcaatg aaatatgata caatcaaaca
    24061 tgtataattg tgtcctttaa aatcgcactg atttcagtta gtgcgatttt ttaccggtta
    24121 ttctcctgaa atatcttctg ttaaacccaa aagatatcaa tagagcaggg ggtggaaaaa
    24181 tggctcaacg gattcctcaa gaggttatcg aaacagtacg cagtcaaacg aatatcgttg
    24241 aggtgattgg acaatatgtc cagttgaaaa agtctggtaa aaattacttg gggctgtgtc
    24301 cttttcatga agaacgtaca ccgtcttttt ctgttgcaga agacaagcag atttttcatt
    24361 gctttggttg tggtaagggc ggaaacgtgt ttacttttct tcaagaacta gaaggtctaa
    24421 gttttccaga agcagtggta aaagtggcag acttcgaaca gataccgtta gaagatcaat
    24481 ggcgtcagcg tactatagct gtccaaaatc ctgaatctgc tacggggaaa ctgattgctg
    24541 ctcatgaaaa agcagcggaa atctatcatc acatgctatt gcatacaaaa gctggacagc
    24601 cagctttaga atatttgcaa agtcgtgggt tgtctttaga attaattgaa gaattcaaca
    24661 taggttttgc acctaatgag cgttcttttc ttcagcaagt attcaagaat gaatcaattt
    24721 caaatgaatt gttagaacgc tctggattgt ttgtcacgca tgatgatggc cgtatgtctg
    24781 atcgttttta tcaaagagtg atgtttccac ttcgtaattc tcaaggccag acgatcggtt
    24841 tttcaggacg ctggcttgca cctgaaaaag ggcaggaaaa agatcagccc aaatacttga
    24901 atagtccaga aacagaactg ttcaataaaa gacaagtttt atttaatctt gataaagcaa
    24961 gaagcgatat ccggaaaaac ggagaaattt tgttgttcga agggtttatg gatgtgattg
    25021 ctgcatggca ggctggtatc aaaactggat tagcttctat ggggacaagt ctaacagctc
    25081 aacagatcca acaaatggaa aagctgacga aagaattggt cttttgctat gacggagaca
    25141 acgcaggttt tgaagcaacc aatcgaggga ttgaattgct tcggcaaaac agtcgcttag
    25201 cactttctgt cgtagtcgtt ccagaaaagc ttgatcctga tgaatatctc cgtaagtatg
    25261 gagaagcatc tttccaagaa ttagtggctc atgggcgcga aacagtcttt acttttaaat
    25321 cacgttattt aaaacaaggg aaaaatttag ataatgaaaa agacaagatc gaatacctcg
    25381 aagaattact agttgaactt agccgagttg tttcaccaat cgaacaagat atgtatctat
    25441 ctcaactgtc aacagagttt cagatcggca gagagacgat acaaaaacaa ctgcgcgatc
    25501 ttcgaaggac aaatcagcaa aatcgtcaag aacctcagga aagtcaacac actcaggtta
    25561 cgagacaaga aactgcacgg aaaaagagac ctttgacgca agtagaaaag gcagagcaga
    25621 ttctattata tcgtgcattt aaagaagttt ctgtccgtaa tattttaaaa gaacgagaat
    25681 ttcagtttat tcatgatgca tatgcagaag tttatttttt attcgatgca tatgtctcac
    25741 aacattatga attcaacctt gcagagtttt tagacttttt gcaagatgaa aatttaaggc
    25801 ggttggtggt ggaaatcgaa tatttaccgg tggcagaaga aagttcgcca cgagaaatcc
    25861 aagacttgct agatgtgatc agcaaatctg gcttagctga tgaaataaca ttaaaaaaac
    25921 aaatgcagca agaagccaga cgaactggaa acaaacagct tgagcttgaa ttgacgattg
    25981 aaatcatcaa tttaacgaaa cagttaaaac aagccaaata gctactcaag gggggccttc
    26041 ttttatggca aatggaacag ataaaaaaac gtatgaagca gcagtagcag ctttcatcaa
    26101 agaaaacaaa cccaaacgac aagttgtata tgatgattta tccaatcagc ttgctacacc
    26161 attcactttg aatgcagatg aaatggacaa attgatccaa aagattgaag acgcaggtat
    26221 cagtgtggtg gatgaaaatg gtgatccatc aattcacagc ttgaagagtg ctgagaaaaa
    26281 agctgaacaa gcaaaaacag aagatctatc tgcgccaacc ggtgtaaaaa tcaatgaccc
    26341 ggttcgtatg tatctgaaag aaattggccg tgtctccctt ttaactgcgg aagaagaagt
    26401 agcgttagca ttgaaaatcg aagaaggcga ccaagaagcg aaacagcgtc tggcagaagc
    26461 aaaccttcgt ttagtagtaa gtatcgctaa acgttatgta ggacgaggca tgcagttcct
    26521 tgacttgatc caagaaggaa acatgggact gatgaaggca gttgaaaaat ttgactaccg
    26581 caaaggattt aagttttcaa cctatgctac atggtggatt cgtcaagcca tcactcgagc
    26641 aatcgctgac caagcacgta cgatccgtat tccagttcat atggttgaaa caattaataa
    26701 attgatacgt atccaacgtc aattgctgca agaccttgga agagaaccta cgccagaaga
    26761 aatcggtgcg gaaatggatc ttccaacaga aaaagtccga gaaatcttga aaatcgcgca
    26821 agaacctgtt tcacttgaaa cgccaatcgg tgaagaagat gactctcact taggtgattt
    26881 catagaagat caggaagcaa caagtccagc agaacacgca gcttatgagt tactaaaaga
    26941 acaattggaa gatgtactag atacattgac tgaccgggag gaaaatgtcc ttcgcttacg
    27001 ttttggttta gatgatggcc ggacgagaac attggaagaa gtagggaaag tatttggtgt
    27061 tactcgggaa cgtattcgtc agatcgaggc taaagcatta agaaaattgc gccatccttc
    27121 tcgttcaaaa caattaaaag atttcttaga ataagcatga actattgacc agaagagaaa
    27181 tcagtaaaac tgtttggttt attttaaaag aaaaaaagct tgagactacg cagtctcaag
    27241 cttttttttc tataggaagg aaattcattc attattttta agtaaatcct aaaaataagc
    27301 tattggcgtg acgtatagaa tatgatagat taaatggtag cgtaaaccta gtaaaggagc
    27361 cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg aaaaaaatgg agcagaatac
    27421 tatctgtcca gactgcggtc atcaaataac tgcagaagat cagcaaataa aagaatcaat
    27481 tgaagaaaca aatacagatg cagctatgca ggataaatca actgaagatg cagtctttcc
    27541 tgatacagaa ttaaacgatc caatcgaatg gtcggaattg aaagatttac ctctagaatc
    27601 tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa gaaccacctg ctcacgatca
    27661 agtaactaat atagagaaaa atgaacagaa gaaagcaact gttcatgaga cttcggaaga
    27721 atcaggaaag aatcaagaag aaacgcttga atcaaaacaa aaaagaagag tagctgaact
    27781 gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta tccgcttata tcaaagcaca
    27841 tcgtgaagat acaaaagaag agcacgcgaa agagctgctc aaaatgatca gtgaaaaaat
    27901 tgcacaggaa aacaaagtag agccagaaca tgaagctgaa aaagacactg aaaaagacac
    27961 tgaaaaagac attagtggag taaacgctgc aggtaaagaa tcattaaaaa cgaatgatct
    28021 aaaaacgaat gatctaaaaa cggatatccc ggaaaaaact gaaacattgt ctgaaaaaca
    28081 atcagaagac gagaaatcga atgggttgcc ggaagcagat aaaaaagaaa tgctcgaaaa
    28141 agcagacaag aaggcaacag agtcggatgg aataaataat gaagccgatg ctgaaactca
    28201 atctgcaagt gaagatagca ctgctcttga agctaaaata gaaaaagaag agaaccagcc
    28261 tgagctgatt gaagcagaaa cagaaccaat aacaatcgtg ccaaatactg aaaaaacgcc
    28321 tcaagaatca aacccagaaa aaaaagattt agataagaca caaaagaaaa aatcaaaaaa
    28381 agtgccgtat ctcgctttag cagcagttct tctactaggt gggggaggct gggcttatta
    28441 tgaccatcat caaaaagtag aagccgaagt agcagcagaa gcaaaaagac agcaacaaat
    28501 ggaagaactt catgagaaac ttgctgcctt ttatacagat gatcaccatc aatttattcg
    28561 tgcagatatg attcatcaag atttatcaaa actaaaaaat gatttatcca gtcttaagaa
    28621 tgaaaaagga tattcagagt tagaaacaac ttaccaagat atccaatcta agatccgaga
    28681 tatccaaaaa gtgaatgagt ggttcatagc acctgccatc gtcgatgatc atctagcaga
    28741 tgacatcaag ttgaaagcag ataaagagat ccaagcaatc gaaacggata acacagattt
    28801 tggaaaattg attcaacaag cacaaacaga agctaaaaat caatatgctg aattgcaaac
    28861 agctaaagaa aaaacagcta tagtctttaa agacggaaaa gtagtggatt cagcaacacg
    28921 cgaccaatac aagacggcaa aagaagcagt agaaaaagtg aaaaatacag atctatctgc
    28981 tcagttgaat gaacaattgc aacaagtaga taagatgctc actgataaag agaaaaaaga
    29041 agaagaggaa aagaaaaaag cagaagaagc gaaaaaagca gaaaaagcga aaaaagcagc
    29101 tgaagaacaa gcaaaacaag ccgctcaagc agcagctgca gctcaagccg ctcaacaagc
    29161 tgcggtcgca caagcagcac agcaagaagc ggcatcctcg accccttcct cttcaaactc
    29221 agcgaatcag ccaatcaaat ctgccagagc aagtgatatt gccgatgcat ctaatccagc
    29281 atggacgtgg gcaccaggtg tccaaagcaa agtgattgct acatgtatcc aacgtggata
    29341 tatcgttgaa ggaggataca gattagaaaa agccagaatc gaaaatgggg aaggctatta
    29401 caatctatat gccacttcaa caaaatctgc attgatgaaa ggcattggag aaagtgcttt
    29461 gccattttac atcgtaacca tcaactgtaa aactggatgg ttcggaggaa atgggagcaa
    29521 ttaattttag gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa
    29581 aggggagtat gacacaactg ttgtcatact ccttttttca cctttccaaa ccaaggctca
    29641 gctattcaag tagataacat tatgttatga tagcaaagaa atgtattaag aaaacctttc
    29701 actatagagg gagaaaaaaa tgaacgattt attagcaact atatttacag gtattattat
    29761 tgatgaaaat gaaaaaaact actttgtcca aaaaaacgga caaacctttg gcttaagcaa
    29821 atcagaaggt gaacacaaaa tcggtgaagc agtggaagga ttcggctacc aaaatcaaaa
    29881 acatgaaaat cggatcacta cacaaattcc taaaagccgt atcggtcatt acgcttttgg
    29941 aatcgttact gctacacgaa aagatttagg tgcgtttgtc gatatcggtc ttccagataa
    30001 agactttgtc gtatctcttg atgaattgcc gacaatgaca gagctatggc ctaaaaaagg
    30061 ggatcgcttg atgatttccc ttagagttga tgcaaaagac cgtatttggg gcagtttagc
    30121 agatgaaaaa atattcaaat ctttaagcaa atatggaaca gaagaaatga aaaacaaaga
    30181 tgtgatagga acagcatacc gcttgaaatt aacaggtacg tacgttttaa cggaagattt
    30241 ctatcttggg tttatccatc cttctgaacg atataatgaa cctcgtctag gcgagcaaat
    30301 aaaaggtcga gtgattggtg tccgaccaga cggtgtactg aatctttcat tgaagccaag
    30361 agcatatgaa gctatatcag atgatgcaca aatgatttta accattttag aacgttctgc
    30421 agatcagcaa atcaacttta cagataaatc ggatccagaa gaaatcatga aagcttttgg
    30481 tatcagcaaa ggagcattta agcgcgcatt aggtaatctt ttgaaacaag gattgattac
    30541 acaagaaaac ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc ttgcatgttt
    30601 ttcttaacta gcttataata attttaatta acatagtgtt atatcttttt ttatttgtaa
    30661 ttattataaa ttgttggggt tgagtaaatg gtcatgaatg ctatttcggc gatgaaaaaa
    30721 acaaaacagc agctacatga agcggggttt aagctgacac cgcaaagaga agctactctg
    30781 cttgtgcttt tggaaaatga gaaagagcat ttatctgcag aagaaatctt ctttttagtg
    30841 aaaaaaaaga gtccggaaat tggacttgct actgtttatc gaacccttga aatcttgaca
    30901 gaattgaaaa ttgtcgataa agtaagtttt aatgatggat tagcccgtta tgatttgaga
    30961 aaagaaggcg ccaaacattt ccatcatcat ttactatgct tgaactgcgg cacgatcgag
    31021 gaagtagaag aggatctttt acttgaagtg gagcaagtag tagaagaacg ttatcatttt
    31081 ttagtaaaag atcatcggct aacgttccat ggaatctgtc aggaatgcta tgaaaaagaa
    31141 aaaagaaacc agtccgtctg agtgtctaga cggctttttc tttgctcaga ttatggaaaa
    31201 agactatcag aatgaagcgg tatctgatat gatagagagg catgaggggg attattatga
    31261 aagagcaaat caacgattat ctccactact tgaatattga acggggattg tccgccaata
    31321 cacgacaaag ctatgaacgt gatttggaac agtacttgca ttttctacag gagcagaaaa
    31381 ttgcttcatg gcaggatgtc gatcgctttt tagtcatccg ttttctggag aagttgcatg
    31441 aagaaaaaaa agcgacagca actgttactc ggatgatctc aagtctcaga agattccatc
    31501 agtttttacg gcaagagcgt atcactgatc atgatccgat gcaacatatc gatacgccga
    31561 agaaagcaca gaaattacca agtacattga ctctgacaga agtagaaaag ctgattgaag
    31621 cacctgatac gacaaaaacg ttagggataa gagatcgtgc aatcttggaa gtcatgtatg
    31681 ctacagggat gcgcgtcagt gaattgattg gattgaaatt gggagatctt catctttctc
    31741 ttggactggt acagacagtt ggaaaaggag ataaagagcg gatcattcct ttaggtgatt
    31801 atgcgatcca atggctggaa cgctacttag acgaagcacg tccattactt gtaaaggacg
    31861 catctgaaat gcatgtattt gtcaataatc atgggaaagg tctttctcgg caaggaatct
    31921 ggaaaaatct gaagcagcta gtacgagagg ctgggattta caaagaagta acaccacata
    31981 cgctgcggca tagctttgcc acacatctgc tagaaaatgg agctgatttg cggacagtac
    32041 aggaattgtt aggtcacgcg gatatctcta cgactcagat ttatacacat atcacgaaga
    32101 aaagaatgac ggatgtttat aagcagcatt ttccaagggc ttagtcatag gaaggtgaga
    32161 agatgttaac acattaccgg aaagaaaacc gtaaaattgc tatgggacta ttgagtttcc
    32221 ataagaaact gacaggtaaa acttctttgt tgaaggagat tgatacgtac gaatctgaag
    32281 acgattatga actttttttc tttacgccag aaggttcaac caacattcaa gggattattg
    32341 gtgtttactg ggcaacgaac gatcagctga tcatccatga catttctttg aatccgtctt
    32401 atcgcggtga gaatatgggc tttcaaatgt tagatgaatt acaggaaaag tacccggatg
    32461 tctccatccg tgcaactgaa atcacagagc cttacattgg aaaatggcta gcaaagaaaa
    32521 agaagaaaga gtgaatgagt tgacggagat caatattaaa ttagatgtat tcgaaggtcc
    32581 tctcgatctg ctgcttcatc tgattcagca aatggaaatc gatatatatg atataccgat
    32641 cgctacgatc acggagcaat atatgaatta tatccataca atgaaaacac tagagctagc
    32701 tgttgcagga gaatacttag tgatggctgc tacattgatg gcaatcaaaa gcaagacatt
    32761 gcttcctgtc caagaattcg tcacagatga tgaagaatta tgggaagaag atcctcgaga
    32821 agaattggtc actcagcttt tagaataccg taaatataaa tatgcagctg aaagactgac
    32881 agaaaaagca gaggaacgaa gtctttatta cacaaaagaa cctatgaaca tcgatgggtt
    32941 agcggatgct gaaaagccgt tgaagcgagg acaagtcaaa aaaatcgatt tgatccgtgc
    33001 catgcagaaa atcttagaac gtaagaaatt ttctcaaaaa gtggaaaaaa cgatcacgcc
    33061 cgatgacaca acgatcgaag aacgaatggt atttatcgaa gagcgtataa aaagaaatgc
    33121 gcatggctgt actttcgatt ctttttttga gtcaccttct aaaagtgaag ttgtcactac
    33181 ttttatggct ttgctagaat tgatgaaaaa agggcagatc attgcgcaac aaaaagaaaa
    33241 ctatgaatca attatgcttt atccagcagt aggagatacc gaacatgacg aaattaagtg
    33301 aacttgaagc ccttcttttt attgttggcg aggaagggat cagtaatgaa gaattaagcg
    33361 gactgttaaa tctcagcgaa gcagaagtca caagtttagt gaaagaatta aaagaatctt
    33421 acactcaaaa ctcgcactca gctttgcata tttttgaaac ggaaagccat tatgtattaa
    33481 cgactaaaaa agagctggct cccttactta agcgatatgc gcaagggaca tcaaacacat
    33541 tatctcaagc cgctgtcgaa accttagcaa tcatcgctta taaacagcca atcacaagag
    33601 ctgaaatcga gcagattcga ggagttcagg tttcaggagc ggtacagcgt ctagctgccc
    33661 ataaactgat tgaagaaaaa ggtagagtag aagggccagg tcggccgata ttatatggaa
    33721 caactggcta cttcttagat tattttggtt tgaaatcatt agaagaactg cctgatacac
    33781 aagaaatgga aatttcattt gaagaagacg tgtcaccaga tctattcttt gatggaatga
    33841 ataaaggaga ataattgtaa tggaaagatt gcagaaagta attgcccacg ctggcatcac
    33901 ttctcgtcga aaagcagaag aattcatcct tgcaggacgt gtaaaagtaa acggacaagt
    33961 tgtaagggaa ttagggataa aagttgggaa acatgacact gtggaagtgg atgaagtccc
    34021 aatttatcaa gaagaatacg gttattattt attgtacaaa cctaaaggag ttatttctgc
    34081 tgttgcagat gacaaaggaa gaaaagttgt tacggatctt ttgccgatgg taaaagaacg
    34141 tatctatcct gtaggtcgac tggattacga tacttcgggg atcttgctac tgacaaatga
    34201 tggagatttt gctcagcgcc tgacacatcc aaaacatgaa gtggataaag tttacgttgc
    34261 aaaggtaaaa gggatcgcta caaaatcgat gcttgcacct ttgacaaaag gaataaagat
    34321 cgaaggacga aaaacatctc cagctaaatt ccaaatttta tcagtggatc ctaagacaaa
    34381 ccacagtatc gtggaattga cgatccacga aggacgtaat catcaagtga aaaatatgct
    34441 tcaagcagtt ggtcttcctg tgcaaaaact aaaacgtgaa aaatacggag atctgacttt
    34501 acaaggccta cgtcctggag attaccgtga actgaacaaa aaagaagtca gcagtttgct
    34561 gaaccactca aaatgaacac ttacttttta taagtgttag atgttgcgtt ttttgaatag
    34621 atatgtataa tgaatatgta aacaaaaatt atataaggtt cgtcttcagg ggcagggtga
    34681 aattcccgac cggtggttat agtccacgac ctattttgca aaaaatagct gaatcggtga
    34741 aattccgata ccgacagtat agtctggata aagaagatag agcttatttg acgacgcttg
    34801 atcaaataac tctaactcta ttttccctgg aaaatagagt tttttcattt ggagagaaac
    34861 gtcaaaaagt tcgctgaaga ggagtcctaa caggaggatt tcaaatgaag aacacccgtg
    34921 tacagaaaat ggttgcagta gcgatgtttg ctgcgatcgc gttagtttta cagtattttg
    34981 cttttccgat catgccggct tttagttttt tgaagatcga ttttagtgat atcccggtat
    35041 tgatcagcat gttcttattc ggtccagcgg ccggagttgc aacagcgttc ttgcgttcag
    35101 tattacatct gctgactaca ggtttttctc cggataattt ggtaggagac actgctagtt
    35161 tcttatcaac aacgatcttt acattcccga tttattattt ctttagtgta aagaaacagc
    35221 ataaaggaag aaacaaattt ttaggtgtca ttacaggaac catcgctcta actattttta
    35281 tgagtatcgc aaattatttc gttattacgc ctctttactt aatgttttta ggactgaacg
    35341 caacacagat gctaggcatg tctttagcaa actatgtact gattgggatc gttcctttca
    35401 acttgatcaa aggattcatc gtgagcgctg tctttttagt gctgcatgcg caacttcttc
    35461 catggttaag tcgaaaacaa catcagttcg aacaacgtca tacagcagtc aaataattat
    35521 caaaaagatc aagaaatttc ttggtctttt tttttggaaa aaaatacaag acatgcaata
    35581 atgtaaacgt tttatttgtt tgttttgagt atttttttga tttatgcata aaaatcaatc
    35641 aattctttgc aaatgtgaaa ttcttctcct tttaaaagac gaaaatgttt gtttataaaa
    35701 aaaaggaaac gctacaatga aaaagaagaa agctttaaaa acctgaaaaa agaggaggga
    35761 atagggaaaa acgaactttt tgaacaaaag ttttacaaaa tgcgtgttat tatttttgtt
    35821 ctgatactct ttttgataaa ataacattag agccaaaatt gttgtctcta acggaaaaga
    35881 aaggaagaag aagatggaac aaacaaaaaa gaaaaaattt caaatgcctt cagcgtatac
    35941 ggtcttattt attattattg cagtaatcgc tgtacttact tggtttatac cagctgggca
    36001 ctatagtgta gacgatgcag gcaacatcgt cgctggatca tatgaacgag tacaacaaaa
    36061 tcctcaaggg ctgtgggata ttttcatggc gccaatcaat gggatgttag gtgtaccagc
    36121 gggggagcta actaaagaaa cacctgctgc aatcccaatc tctttcttca ttttggttgt
    36181 tggtggattt ttaggtatta tcaacaaaac aggggcacta gatgccggga ttgcttcagt
    36241 tgtgaagaaa ttcaaaggaa aagaaaaaat gcttattcca gtattgatgc tgttatttgc
    36301 attaggcggc tctacttacg gaatggcaga agaaacgatt ccattctatg ctttactgat
    36361 cccagttatg atggctgtag gctttgatac agtagtagca gtagcaatcg tattagtagg
    36421 gtctcaagta ggctgtctag cttcaacggt aaatcctttt gcaacaggcg tagcttcaca
    36481 agctgttggg atttcgatgg gtgaaggtat tggtcttcga ttcttgatgt tcgttatttt
    36541 gttagctatt tctattgtat atgtgtatcg ttatgcatca aagatcgaaa aagatccaac
    36601 aaaatcatta gtatacaacc aacgtaaaga agatatgaag catttcgata ttgaagcaat
    36661 cggtcgtcaa gaagtaatca cgaaaaaaca aaaacatgtt aatgtattgt tctttattac
    36721 ttttggtatc atgatcatca gtttgattcc ttggacaacc ttgaatgcga atttcacgat
    36781 ttttgaatca ttgacaaact ggttaacaaa ccttccggta ctaggtactg ttctaggtaa
    36841 aagcatgctt ccattaggtg actggtactt cccagaaatt actatgttat tcttagtaat
    36901 gtccatcgta gtagcaatca cttatggcat gaaagagtct gaatttgtct ctgaattttt
    36961 agctggtgct tctgatttga tcggtgtagc aatcgtagta gctgttgctc gtggtatcca
    37021 agtggttatg aacaatggat taatcacaga caccatctta cattggggcg aacaaggatt
    37081 aagttcatta tcttcaagtg tattcatcat tattacattt attttctata tccctatgtc
    37141 attcttgatt ccttcaactt ccggcttagc tgctgcaaca atgggaatca tgggacctat
    37201 gggtgaattt gccggcgtcg gtaaggattt agtaatcact gcttatcaat ctgcatctgg
    37261 tttagtaaac ttaattacac caacatctgc tatcgtaatg ggtgctgtag caattgctcg
    37321 tttcgatgtt tcagtgtggt ggaaatttac cggtaaatta attgcgatat tatttgtagc
    37381 catctgtgtg atcctaggtg tagcagcaat gttctaaacc tagacaaata caggtacaag
    37441 gtctatcata agaatgaaga aaccgataaa cggctatcga taagcacgtg gacaagtcaa
    37501 tagctccatt cagtgagtgt tttttctttc atcatttgtt aagaacattc tgcttgctga
    37561 tggagcaggc ttaaaacgtc attatttgag aagaagaggt tgtgtcggag gaatttgaca
    37621 tgaccctttt taacatatta ggaggataat atcatgaaaa aatttgtgac agaacaacat
    37681 catgaacaag cattagaatc attgagcgaa ttgatccgaa ttccttccgt actagatgaa
    37741 gcagattcag gacaaggaca tcccttcggt aaaaaagtga ttgaagcttt ggataaagta
    37801 ttggaaatca gtgaaaagct aggatttaga acatttaaag atccagaagg ctattacggt
    37861 tattcagaga ttggctctgg agatgaatta ttcggtatct tgtgtcatat ggacgttgtt
    37921 ccagcagggg acgaaaataa ttgggaaaca aaaccattcg atccaactat aaaagacggc
    37981 tggttagttg gtcgcggatc ccaagatgac aaaggtcctt ctattgcggc aatgtatgcc
    38041 gtcaaagctt tgattgatgc tggcgtggaa ttcaagacac gtattcgttt tatctttggt
    38101 acagatgaag aaaatttatg gcgttgttta gaaaaatata acgaaaaaga agaaggaatt
    38161 acgcaaggat ttgcgccaga tgctgaattt cctttaattt atgcagaaaa aggcttattg
    38221 caagcttact tgacaggacc aggaacgaac gagttttctg taaaagctgg aggagcgttg
    38281 aatgttgttc ctgatgcagc tccttactct ggagaaaagc tttctgaagt aaaagaagcg
    38341 ttgaaaaaac acgaattcga tttcgaagaa caaggtgaag gcatcgtcgt tcttggtaaa
    38401 agtatccacg cgaaagatgc agcacaagga gtgaatgcta tttctcgtct ggcaattgct
    38461 ctttcagaag tattcgattt tggcccaatc aatttcttag gaaaattagt tcaagaaaat
    38521 gcaactggtg aagctgtcgt gggcaaaaca gaagatgaac aatccggcga gctaacgatg
    38581 aactttgcaa gtttggaaat cacaccagaa caaacaaaaa tcggtgtaga tatgagaatc
    38641 cctgttacat tcaagaaaga tgatttagta gctaaattaa ctaaaacagc agaaaaatat
    38701 gggttgactt atgaagaatt tgacttcctt gattcattat acgtgccact agacagtgag
    38761 ttagtgaaaa atctattggg gacatatcgc gatattactg gagacatgac tgaaccgttt
    38821 gtatcaggcg gcgctacttt tgctcgtacg atgaaccagt gtgtggcatt cggtgcgatg
    38881 ttccctgata ctcctgactt tatgcaccag gcgaatgaac gatgggaatt aagtagtatg
    38941 tataaagcaa tggaaattta tgcagaagct gtgtatcgtt tatgtgctaa ataaaaataa
    39001 acatacaaaa agggcggata ttcaaatgga tattccgtct ttttttattt gatgctggaa
    39061 agtttgcatt tctctcttat tttttttaag gtataatcat acttgattaa gaaaaagggg
    39121 tcactcagat agaaaaggtg ggagaaagtg aaaatattta gacgaatact aattggctta
    39181 gtcatcattt taggattact cggtggagcc ggttattact atatacagca aaatacgtac
    39241 gaaccatcgt cagaagcgca agtgttggta aaagaaggaa aaaacaaaga agattactta
    39301 ttttttgaag gagagaaaaa gaatcatgga aattctccat tacttatttt ttatcctggt
    39361 gctctcgtag agccagaaag ttatagtatt tgggcgcaag atttagcaaa aaacggcttt
    39421 tcagtcgcta ttgtcaaaat gccacttgat ctagcagtga tgggcggaaa caaagcggat
    39481 aaggtcaaag aagattttcc aaattccgat tatgtgatcg gtggccattc attaggtggc
    39541 gtaatggcaa gtcgttatgt caataagaat cattcagaca aacatttaaa aggggtattc
    39601 ttcatggcaa gttatcccga caaaaaagga acattgacag aatcagctgt tcctgtttta
    39661 tcgatcacgg cgacaaaaga taaagtattg aatcaggaag cttatcaaga agctaagcag
    39721 tatttaccgg aaagaacaga gtatgtatca atcaatggcg gaaatcatgg aggttttggc
    39781 agctatggtg agcaaaaagg agatggagag gcagaaattt ctaacgcaga ccagcaaact
    39841 gtgatcgtca atacattgga aaactggctg aaaaagttga aataatataa atgggtccta
    39901 tagggtccgt gaaaaaacta gtcaattaac tatagatagc aaatgaatct ttcaaaataa
    39961 gtcgggattt cacggaacaa gagaaacaga tcccggctta ttttcaagtt tagagaactt
    40021 agtgtacgtt tttaaagttt cgtttatatt tatattttat tttctaataa aatcgctcga
    40081 actggacatt tttgatacgc tttttttaca tatgtttctt cttccggcat cacgaactgt
    40141 tgatgagcct ctggttcttg ggcaaataaa acgattcctt cttcatcata atcgaaaata
    40201 tctggcgctt cgatttgaca aagaccgcat gcaatacagc gttcaggtac aagtttacat
    40261 agtagtgaca tttctaacca tcctaactta aggagaataa tatgattgtt tttattttat
    40321 cgctattttc atcaggacac aagctgcgga ttagttctct ttaccaactc cttgtgggaa
    40381 aacgaacgac ttcagtactt atttatggat ttacccacga acttttgttt atccataaca
    40441 gttttccaga attgaagcag gataagtttt atcaaatttt gcaaaaaatt gcgcagcaag
    40501 gttggatcga aataaatgaa aatgaagcaa agctaacgcc tgcaggggct gatatgttgt
    40561 ccgaacatca aatagaacat actggtttgc gttttgaccg ctatggcaga acaggggaaa
    40621 ctagttggcg gctcattaaa tttgctgttc aagtaatttc taatctggca acgggtattc
    40681 aagactatct tccagcagag acaagtcctt tttatacttt ccaactaaaa aaatggctta
    40741 gtgaaagcca attgccacgt gaaattttga ttgatactgc gtatgagagt ttggtgcagc
    40801 tgttttcgga gataccagaa gaagcagcag attttttagc taaccaatta tctggaaatg
    40861 agcgaacagg gctgcttcct tatcagctgg caaaaaacaa tgatgagtca gcggtttatc
    40921 tgcaacaaag cagatgtatc caccttttgt tagcacaaat cgaaaaacgg ccagattcgc
    40981 tttggcacgc tttgatcgat tcattattac agcaaaattt caatcaaagc atgatgatca
    41041 ccaagcagat gtttatgaat ggtcagacaa ttgaccagat tatggcaatc cgtcatttaa
    41101 aaagaggaac agtaacagat catcttattg agtgggcctt gttttttgat gattttccat
    41161 acgagcggat tctctctgca gaaacaatcg aacgtttgga accaaataag gattccgtgc
    41221 gagagtggcg ctttagcgaa tggaatgtag atggacaatt agattatgga gaatttcgtt
    41281 tgtatcaaat ctatctattg agaaaggaag cgatacagaa tgttaataaa tgaattaaaa
    41341 aaatattttg gatttgattc atttcgtccc ggccaagaag aaatcattca ggcgttgctt
    41401 gatggagaag atactctcgc tattttgcct acaggaacgg gaaaaagcct ttgctatcaa
    41461 ttgacggggt ttttaatgga aggtctggtc atcatcgtgt caccgctgct ttctctaatg
    41521 gaagatcaag tgacgcaatt acaaaaacga ggggagaaac gagtagctgc tttcaatagt
    41581 ttactttcgc aatctgaaag aagatacgtc ctccgtcatc tatcgcaata taaatttttg
    41641 tttttgagtc ccgaaatgtt gactaatcct tctcttttag agcatctgaa aaaacaaaat
    41701 attgcgttat atgtagtaga tgaggctcat tgcgtgtctc aatggggagt tgatttccgt
    41761 ccagaatatc aacagttagg aaaaatccga aagcttttag gaaacccggt cacactggca
    41821 ctaacggcga cagcaacaga tttagtagca agagatatac gtcatgtact atttgatcga
    41881 ccaccaaaag agatcagaca atcagtcaat cggcaaaata tctcgctttt tgttcggaaa
    41941 acgcagcaaa aagaacaaga acttgaacac ttcatggaaa gagcacaagg agcggcaatc
    42001 atttattgtg caacaaaaaa agaagtcgaa cgattgtatc atttattcag ggaacgtttt
    42061 acagttggat actatcacgg tggtcttgat gcagctcaaa gaagacaact tcaacagcag
    42121 tttgtaaaaa atcagctgca gtttctcatt gctacgaatg catttggtat ggggatcgat
    42181 aaatctgata tccgttatgt gatccattat gatttgccag acagtctaga aaactatgta
    42241 caagaaatcg gtcgagcagg cagagatcag gaagaaagtg cagcaattct tttttatcaa
    42301 agtggggatg aacgtatcca ttatttcttt aatcaacttt ctagagaaca gcgacaaagt
    42361 tttgagcttt atttggaata tgccacagag caagctccct ttgatgagct gcaaaaaaaa
    42421 tggatggagc tgatccagca gtctgaaaaa ccagaaaatt gggtggaacg gctcaaacat
    42481 caggaaaaag aaaaagaatt ccgcctgcag cagatgcttc gctatattaa tgaagaaaac
    42541 tgccgcagaa aattcatctt agcttatttt ggcgaaaaac tctccgaaaa gccgcagaat
    42601 tgttgtgata ttgatggagc aaaagcagaa cttctgccaa agaggaagct tttttctaag
    42661 actgaacaac ttcattggga aacgatttta ctaaatttat ttaaaaaaaa catggaacat
    42721 tgatttttta ggacaagaca ctttttccta tggtataata cacacgatag agtttaggag
    42781 gtaaaacagt gagcagaaaa gatagacatc aatctacaga aaataacgaa acacaagaac
    42841 catgggaaca accaatctac gatacagatg atgaagtttc atcaagaagt tctcagagac
    42901 aacaaaagaa aggaaataca ttatttcttt cgctattttt gattttactg gtcttatgta
    42961 tcgctattcc agcaggtgct tatttctgga tcagaaatgg atcaagcaac aatgcaacag
    43021 cagcaacttc ttctgaaact tcgacatcag tagtagaaag ttctacaagt acaacaaaag
    43081 aggagtcaac tgagacttca acagtagaat ctagtacaat cgcggaagaa tctactgtgt
    43141 ctcaagatac aggtgtaacg tcagaaagca gtattgcagc agagacaaca ccgacttcct
    43201 caagtgaggc aacttatcaa gcaggggatt caagcagcaa tgcagcagaa acaacacctt
    43261 cttcatcagc tgcagccggt tcaacaacga ctgttcaagc aggcgaaggt ccaaaacaag
    43321 tggctgagcg tgcagggatc accactgatc aattgtttga attgaatggc ttagatccaa
    43381 acaactttat gctttaccct ggtcaagaat taagaattaa ataataatag ccgcgctttg
    43441 cggctttttt ttgagaaaaa ggagttatat catgacacaa atcagtattg caatcgacgg
    43501 gcctgcttca tctgggaaaa gtaccgttgc caagatatta gcaaaagaat tgaattatat
    43561 ttatacagat accggtgcca tgtatcgcgc agtaacttat ttagctatta cgaacaatgt
    43621 atccttttca gatgaagaag ggctagtttc gctgatcaaa aagtatccta tctctttttc
    43681 gcaacaagaa gatggacagc atgtattcat agcatcaaaa gatgtgactt tagaaatccg
    43741 tcaaccagat gtcacaaaag ctgtttctga agtctctgca catggaaaag tcagagaaga
    43801 attagttgct atccaacgaa aaatagggga acgcggtggc gttgtcatgg acggaagaga
    43861 catcgggaca gctgtattac cgaatgcaga agtgaaaatc tttttagtag caagcgtaag
    43921 tgagcgtgca gaaagacgtt tcaaagagaa ccaagaaaaa ggaattccga ctgatcttga
    43981 aacactgacg aaagagatac aggaaagaga ccattatgat tcaacaaggg acgtctctcc
    44041 gctgaagcaa gcagaggatg ctgttcgtat cgatacgact ggcaaatcga ttccagaagt
    44101 cgttgcagca ataaaagctg ttgttttgaa aaaaggttat cttctttttt aaattaagaa
    44161 aaaaaatgaa aaacaattgt tttttcaagg aaaacgaacg aaatcgcttt attttttttg
    44221 aataatcaat taaactaaaa acagtaccgt acttacggca gaatgttggt taggaggaca
    44281 agtgttatga cagaatttga tcaaaatcaa gagaacaacc agtcaatgga agacgcaatg
    44341 aaaagcgtac aagaagtaaa agttggcgat gttgttaagg gcgaagtttt agtcattgaa
    44401 gacaaacaag tgattgtcgg aatcgaaggg gctggagttg aaggagtcgt tccggcaaaa
    44461 gaattatcaa ctttgccggt agaagatatc agcgaagtag taaaagtcgg tgatgtatta
    44521 gacttagtcg ttatcagtac gatcggcaaa gataaagaaa acggcagcta cctactttca
    44581 aaacgccgtt tagatgctaa aaaagtttgg gaagatatcg aaagagattt ccaagagggc
    44641 aaggtgatcg aagcacctgt tacaaatgtt gtcaaaggcg gtttagtcgt tgacgtcggt
    44701 gtacgtggtt ttgtccctgc atcaatggtg gaagatcatt ttgttgcaga cttttcagac
    44761 tataaaggca aaacattgag ctttaaaatc atcgaaatcg aaccatcaga aaatcgtttg
    44821 atcttatcgc ataaagcggt agtggaaaac gaaagagaag aaaagaaaaa agaaattctt
    44881 tcaactatcc atgagggaga tgttctcagt ggacgtgttg cacgtcttac tgattttggc
    44941 gcatttgtcg acctaggcgg aattgacggt ctagtccatg tctcagagat ttcacatagt
    45001 catgtagcaa aaccaagtga tgtattggct gtcaatgatg atgtaaatgt caaagtgttg
    45061 tctatcaatc ctgaacaaga acgtgtttcc ttgtccatca aagacacaca gcctggacct
    45121 tggtcagaaa tcgatacaaa agctccagtt ggcacagtat tagacggaac agttaaacgt
    45181 ttgacaagct tcggtgcctt tgttgaagtg ttccctggag tagaagggct agtccatatc
    45241 tctcaaattt cacataaaca tattgcgaca ccacatgaag tgcttcatga aggtgacgaa
    45301 gtgaaagtga aagtattaga gatccatcca gaagaacatc gtattgcatt gagtatcaaa
    45361 gctttagaag aaaaaccagc ttcagaagaa gaaccaaaaa atgttgagtc ttatgaatta
    45421 ccagaagaaa atactggatt tactatggga gacattttag gtgatgcgtt gaaagaagaa
    45481 gtagaagaaa aagcaaccga tgattctgaa aaata
//
LOCUS       DAZUMO010000018        44676 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000018 DAZUMO010000000
VERSION     DAZUMO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.018"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..122)
                     /locus_tag="ACG8ER_000953"
     CDS             complement(6..122)
                     /locus_tag="ACG8ER_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS5049553.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(195..1151)
                     /locus_tag="ACG8ER_000954"
     CDS             complement(195..1151)
                     /locus_tag="ACG8ER_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5049554.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1322..1690
                     /locus_tag="ACG8ER_000955"
     CDS             1322..1690
                     /locus_tag="ACG8ER_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS5049555.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1860..2291
                     /gene="mraZ"
                     /locus_tag="ACG8ER_000956"
     CDS             1860..2291
                     /gene="mraZ"
                     /locus_tag="ACG8ER_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS5049556.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2312..3271
                     /gene="rsmH"
                     /locus_tag="ACG8ER_000957"
     CDS             2312..3271
                     /gene="rsmH"
                     /locus_tag="ACG8ER_000957"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS5049557.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3276..3677
                     /gene="ftsL"
                     /locus_tag="ACG8ER_000958"
     CDS             3276..3677
                     /gene="ftsL"
                     /locus_tag="ACG8ER_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS5049558.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRRVK"
     gene            3678..5870
                     /locus_tag="ACG8ER_000959"
     CDS             3678..5870
                     /locus_tag="ACG8ER_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5049559.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5896..6858
                     /gene="mraY"
                     /locus_tag="ACG8ER_000960"
     CDS             5896..6858
                     /gene="mraY"
                     /locus_tag="ACG8ER_000960"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS5049560.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6872..8233
                     /gene="murD"
                     /locus_tag="ACG8ER_000961"
     CDS             6872..8233
                     /gene="murD"
                     /locus_tag="ACG8ER_000961"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS5049561.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8259..9347
                     /gene="murG"
                     /locus_tag="ACG8ER_000962"
     CDS             8259..9347
                     /gene="murG"
                     /locus_tag="ACG8ER_000962"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5049562.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9362..10582
                     /locus_tag="ACG8ER_000963"
     CDS             9362..10582
                     /locus_tag="ACG8ER_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS5049563.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10738..12063
                     /gene="ftsA"
                     /locus_tag="ACG8ER_000964"
     CDS             10738..12063
                     /gene="ftsA"
                     /locus_tag="ACG8ER_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS5049564.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            12086..13327
                     /gene="ftsZ"
                     /locus_tag="ACG8ER_000965"
     CDS             12086..13327
                     /gene="ftsZ"
                     /locus_tag="ACG8ER_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS5049565.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13344..14021
                     /locus_tag="ACG8ER_000966"
     CDS             13344..14021
                     /locus_tag="ACG8ER_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS5049566.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            14035..14634
                     /locus_tag="ACG8ER_000967"
     CDS             14035..14634
                     /locus_tag="ACG8ER_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS5049567.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14715..14936
                     /locus_tag="ACG8ER_000968"
     CDS             14715..14936
                     /locus_tag="ACG8ER_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS5049568.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14985..15767
                     /locus_tag="ACG8ER_000969"
     CDS             14985..15767
                     /locus_tag="ACG8ER_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS5049569.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15788..16495
                     /locus_tag="ACG8ER_000970"
     CDS             15788..16495
                     /locus_tag="ACG8ER_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS5049570.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16757..19555
                     /gene="ileS"
                     /locus_tag="ACG8ER_000971"
     CDS             16757..19555
                     /gene="ileS"
                     /locus_tag="ACG8ER_000971"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS5049571.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19604..21130)
                     /gene="zwf"
                     /locus_tag="ACG8ER_000972"
     CDS             complement(19604..21130)
                     /gene="zwf"
                     /locus_tag="ACG8ER_000972"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS5049572.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(21145..21792)
                     /locus_tag="ACG8ER_000973"
     CDS             complement(21145..21792)
                     /locus_tag="ACG8ER_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5049573.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21976..22305)
                     /locus_tag="ACG8ER_000974"
     CDS             complement(21976..22305)
                     /locus_tag="ACG8ER_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS5049574.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22482..23210
                     /gene="sfsA"
                     /locus_tag="ACG8ER_000975"
     CDS             22482..23210
                     /gene="sfsA"
                     /locus_tag="ACG8ER_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS5049575.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            23226..24239
                     /locus_tag="ACG8ER_000976"
     CDS             23226..24239
                     /locus_tag="ACG8ER_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5049576.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            24239..25516
                     /locus_tag="ACG8ER_000977"
     CDS             24239..25516
                     /locus_tag="ACG8ER_000977"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5049577.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25579..28281
                     /locus_tag="ACG8ER_000978"
     CDS             25579..28281
                     /locus_tag="ACG8ER_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5049578.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28433..28750
                     /locus_tag="ACG8ER_000979"
     CDS             28433..28750
                     /locus_tag="ACG8ER_000979"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5049579.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28780..29100
                     /locus_tag="ACG8ER_000980"
     CDS             28780..29100
                     /locus_tag="ACG8ER_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5049580.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            29208..30668
                     /locus_tag="ACG8ER_000981"
     CDS             29208..30668
                     /locus_tag="ACG8ER_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5049581.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30736..30957
                     /locus_tag="ACG8ER_000982"
     CDS             30736..30957
                     /locus_tag="ACG8ER_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049582.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30988..31170
                     /locus_tag="ACG8ER_000983"
     CDS             30988..31170
                     /locus_tag="ACG8ER_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS5049583.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            31170..31583
                     /locus_tag="ACG8ER_000984"
     CDS             31170..31583
                     /locus_tag="ACG8ER_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS5049584.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31706..32887
                     /locus_tag="ACG8ER_000985"
     CDS             31706..32887
                     /locus_tag="ACG8ER_000985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5049585.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(32958..33122)
                     /locus_tag="ACG8ER_000986"
     CDS             complement(32958..33122)
                     /locus_tag="ACG8ER_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049586.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            33276..33349
                     /locus_tag="ACG8ER_000987"
     tRNA            33276..33349
                     /locus_tag="ACG8ER_000987"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33310..33312,aa:Arg,seq:cct)
     gene            complement(33418..34557)
                     /locus_tag="ACG8ER_000988"
     CDS             complement(33418..34557)
                     /locus_tag="ACG8ER_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5049587.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            34729..34863
                     /locus_tag="ACG8ER_000989"
     CDS             34729..34863
                     /locus_tag="ACG8ER_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049588.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(34856..35491)
                     /locus_tag="ACG8ER_000990"
     CDS             complement(34856..35491)
                     /locus_tag="ACG8ER_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049589.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(35604..36239)
                     /locus_tag="ACG8ER_000991"
     CDS             complement(35604..36239)
                     /locus_tag="ACG8ER_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS5049590.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPSTEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(36273..36734)
                     /locus_tag="ACG8ER_000992"
     CDS             complement(36273..36734)
                     /locus_tag="ACG8ER_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFS5049591.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(36864..37295)
                     /locus_tag="ACG8ER_000993"
     CDS             complement(36864..37295)
                     /locus_tag="ACG8ER_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5049592.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(37313..37633)
                     /locus_tag="ACG8ER_000994"
     CDS             complement(37313..37633)
                     /locus_tag="ACG8ER_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5049593.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            37932..38708
                     /locus_tag="ACG8ER_000995"
     CDS             37932..38708
                     /locus_tag="ACG8ER_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS5049594.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            38723..38926
                     /locus_tag="ACG8ER_000996"
     CDS             38723..38926
                     /locus_tag="ACG8ER_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049595.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            38942..39280
                     /locus_tag="ACG8ER_000997"
     CDS             38942..39280
                     /locus_tag="ACG8ER_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS5049596.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            39267..39446
                     /locus_tag="ACG8ER_000998"
     CDS             39267..39446
                     /locus_tag="ACG8ER_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049597.1"
                     /translation="MGKFNRALVFSAPLIIYALELWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(39489..39959)
                     /locus_tag="ACG8ER_000999"
     CDS             complement(39489..39959)
                     /locus_tag="ACG8ER_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049598.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            40046..40744
                     /locus_tag="ACG8ER_001000"
     CDS             40046..40744
                     /locus_tag="ACG8ER_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS5049599.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            40922..41263
                     /locus_tag="ACG8ER_001001"
     CDS             40922..41263
                     /locus_tag="ACG8ER_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049600.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            41256..41927
                     /locus_tag="ACG8ER_001002"
     CDS             41256..41927
                     /locus_tag="ACG8ER_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5049601.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            41933..42619
                     /locus_tag="ACG8ER_001003"
     CDS             41933..42619
                     /locus_tag="ACG8ER_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5049602.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            42622..43371
                     /locus_tag="ACG8ER_001004"
     CDS             42622..43371
                     /locus_tag="ACG8ER_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049603.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            43383..43652
                     /locus_tag="ACG8ER_001005"
     CDS             43383..43652
                     /locus_tag="ACG8ER_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049604.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            43657..43821
                     /locus_tag="ACG8ER_001006"
     CDS             43657..43821
                     /locus_tag="ACG8ER_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049605.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            43814..44116
                     /locus_tag="ACG8ER_001007"
     CDS             43814..44116
                     /locus_tag="ACG8ER_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049606.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            44113..44274
                     /locus_tag="ACG8ER_001008"
     CDS             44113..44274
                     /locus_tag="ACG8ER_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5049607.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            44271..44576
                     /locus_tag="ACG8ER_001009"
     CDS             44271..44576
                     /locus_tag="ACG8ER_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049608.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT        15061 a         7495 c         9126 g        12994 t
ORIGIN      
        1 tatttttaca tgacactcat tacagcagtc aatacaacag atccaatcgt taaaatcagc
       61 attgtccaaa tgacaaattt tgttacttta gcaaaagtac ttgtttgttt tttttcacgc
      121 attgttttca acctcgtcat taattagttg attcaagtgt acaatctttt ttccatttcc
      181 acaagagggc ttttttataa aaacttccct ttttttaaat aataggtagt tacgatacaa
      241 ataaataatg ttaatagaac tagccatata aaggcatcat ccctttcagc aataggtaac
      301 ttaatgttca ttccatagat actgccgacg attgtcggta tagttaaaat aatcgttaaa
      361 gaagttaaaa ttttcataat attattcaga ttattcgata cgattgctga aaaagtctga
      421 ttcatttgtt ctactagttt taattgaatg cgagaagtag tcattgcctg ctctgtttcc
      481 accaatacat cacgtaaatg atttttcaaa gcatgatttt cttggaaaag tttattttgc
      541 gctagttctt tcaatgcatc taaattagca gaagtcgctt ctctgaaata aacgaggctt
      601 ttttggatat ccatgatttg ataaagttgt ttgttttcag tggatacttg aagtttggct
      661 tccaaagtat cgcattgaga ttgaagcgta gctaaataac tgttgtaaga gagtgtgacc
      721 tgccaaatga attgaaagaa gatattgaga ttaggcgaaa tatcactttt tggaaaggtc
      781 tggctaaaga ctttctttaa aaacagtggt tcatgattcg taattgtgat aagcttcttt
      841 ttgggcgtca aaataataga gagcgggtat gtatccactt gaaaataacc gctaggactt
      901 gttttagcaa aaggatattg taacaataat aacgctggct ttgtcaactt tgattgattg
      961 aattcttctg tacgggaatt ttctgcatcg tccaatatgg aagtgatgta atctgacgga
     1021 atttcaaatt gatgtgtcag ctgatgaatc tcttcttgat taggacgttc tacacaatac
     1081 cagatcgttt ggtcagtttc ctttccggta ttacgcagtt tctcattatc taatgtaaaa
     1141 tatttgatca tgattttcct cctgttgcct gtttctttta ttataacgga tagggaattg
     1201 gaatacatgg aatgtgtttg taagatatag aatataactg cataaattta aataaaatga
     1261 aacagttgtg taaagtatat acagtatact tgaagcaacg ataaaaaagg agtttacgtt
     1321 catgggcaca ttcacaccac ttatcttgtt ttggtatatt tacccggtaa tcgttttgtt
     1381 tggttgccaa tttcttgtaa aattattttc gttaaatcgt cgttttaaaa taaaagcccc
     1441 tgatttggcg attccttttt tgtggggcgg tatgcatgca ttgtctagaa atacagggac
     1501 aatttctatc ttaccgtttt tctttatttc tgttttgcta atgggaattt gtattgctgt
     1561 tttccaagca tattattatg aagaaattat ttatccgcgt tatttcaaga tgacttggcg
     1621 tttggttttt ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga acatactttc
     1681 ttacctatga aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt ttgcagcttg
     1741 atttttaaaa aaatgtttat gaataaaaaa agttgttccc ggatttccca gaaaaggtgg
     1801 tagaaagtgg gggattgtgg tagactaatt ttagttagtg gaggaatggg gggctttcaa
     1861 tgttgatggg tgaattccaa cataatattg atgcaaaagg acgtttaatc gtgccctcaa
     1921 aacttcgtga agaacttggc gaaaagtttg ttctgacacg cggattggat ggctgtcttt
     1981 tcggctatcc gatgagcgaa tgggaaaatc tggaagctaa actgaacgaa atgccgttgg
     2041 ctaaaaaaga cgcacgtact tttgtgcgat tcttctattc ggctgccact gaatgcgaac
     2101 ttgataaaca aggacgaatc aatataccca gcacgttaag aaattatgca gctttgacaa
     2161 aaggttgcgt agtcatcggt gtatctaacc gaattgaaat ttgggatgag acacgatggc
     2221 aagatttttc tgcagcagca gaagagaact ttgacgaaat tgctgaaagt atgattgatt
     2281 tcggactata agaataggaa gaggctgact aatgactgaa acatttcaac actatacagt
     2341 catgttgaaa gaaacggtag atggattgaa catcaaaccg gatggcgttt atgtagactg
     2401 tacactcggc ggtgccggac atagtgaata tttattaagc caactaagtt ctaaagggca
     2461 tctgtatgcg tttgaccaag atcaaaaagc aatcgatcac gccaagctgc gtttagcttc
     2521 ttatgtcgaa caaggtatgg tcacatttat caaagcgaac ttcagagaat tagaagaacg
     2581 cttgagtgag catgtagata aggtagacgg cattttgtat gatctagggg tttcctctcc
     2641 tcagctagat gaagcagaac gtggatttag ttatcatcaa gatgcaccgt tggatatgcg
     2701 catggatcag tcagctcctt tatccgctta tcacgtaatt aatgaatatt cctaccatga
     2761 attagtaaag attttcttcc gctatggcga ggagaaattt tccaaacaaa ttgctcgtga
     2821 aatcgaacga gtacgaaaaa caaaaccaat ccaaacaact ggtgagcttg tcgaaatcat
     2881 taaacaagtc atcccagctc ccgcaagaag aaaaggcggg catcctgcaa aaagaatttt
     2941 tcaagctgtg cggattgcag taaatgacga actcggtgcc gaagaggcct ctttagaaca
     3001 ggcaatccgg ttattgaaag tcaatggaag aatcagcgta atcacattcc attcattgga
     3061 agacagaatt gtcaaaagta tgtttaaaga atatagtacg gtacaagatc taccaccggg
     3121 acttcctgtc atacctgacg aattccaacc agaactgaaa gtcattactc gtaaaccaat
     3181 ccttccttca gaggaagagc taacagaaaa caatcgatca cgcagtgcaa agttacgtat
     3241 tgcagaaaaa ataagaatga aagaggagta gaatgatggc agaattaaag aaaatgcaag
     3301 attacgaata tgatttacct gaattggtgg atcagccgga acaacaaatg cctgattctt
     3361 cttcgaatca agaaatcatt cttccacaat caccggcaaa acgattaaag cagatttctg
     3421 ctttggaaaa aattgctgtg gcaagcgtgg cgattgctat tgttgcttta tgtgttttaa
     3481 cagttatgct gcgcacaaat atcagcggag ctgaaaaaga aatcacgtca atacaaacag
     3541 aaatcaatga taaaaagcaa gaaaaaacga gtttagaaca agaaaaaaac gaactttctc
     3601 gtactgaacg gattaaacaa atcgcagaag aaaaaggatt gtctattaat gacgacaatt
     3661 taaggagagt gaagtaaatg agtcttaaaa ataaaattcg gcggtttatg gaaaagaaaa
     3721 atctaaatcc gatgaacaat cgcaaaaaag taggtatcat tttatttgct acgagtattg
     3781 gattgttctt tttatttgcc gtccgtttct cgtatatcgt tattggaggt catgttgctg
     3841 gtacctcatt agctgaaaaa acaaaacagt tgtaccaagg aagtgaagta gtaaaagcaa
     3901 aaagaggaac gatttatgat cgtaacggtg tggcgctcgc agaagatgcc tcctcttatt
     3961 caattaaagc gatactctca aaaacatata cctctgggga taaaaaactg tatgttgaag
     4021 aaaagaattt tgataaaata gcagaaatcc tgcataagaa tctttctata gacaaaaaag
     4081 atgcactgaa tattttagag gacggcgcaa aaaaagagct gtatcaagtt gaatttggta
     4141 gctatgggaa aaatatctcc caagaaacca agcaaaacat cgaagcggac atgaaaaaag
     4201 aaggtgtcgc tggtctttat tttgtcgacc atcaggctcg gatgtatcca aatggcgtct
     4261 tttcttctca ttttattgga tacgcagttc cagacaaaga tgagaatggc ttagttggta
     4321 aactaggttt ggagtctgct tacaatgata tcttgagcgg taaagatggt aagattattt
     4381 atcaaaaaga taattttcaa aatcctctgc caggtacagt ggcagaagaa gaaaaagctg
     4441 tcgacggaca agatatctac acgactttag acagcagact acaaagttat ttagaaacac
     4501 tgatggatca agtaaatgaa gaatatcagc cagaagaatt gacagcagtc ctgatgaaag
     4561 caaaaaccgg agaaatcttg gcaatgggac aacgacctac tttcaatcct gaaacaatgg
     4621 aaggattgac tggaaaagat gctatctggc gtaatttctt ggtacaagat agttatgaac
     4681 caggatcaac catgaaagtt ttcactactg cagctgctat cgaagaagga gaattcaacg
     4741 agaatgaaac cttccaatct ggaaaaatcc aagtagaaga tgctacgatc aatgaccatg
     4801 actttggtga aaaaggtgtg ctgaccatgc gtcaagctct ttcatggtca agtaacgtcg
     4861 ggatggtcat actggaacag cgattaggcg gacgatggta taactattta caaaaactgg
     4921 gatttggaca aagtactcat tctggattag atgatgaagt aaacggtgct ttacctacat
     4981 taaatattgt cgaccgagca atgagtgctt atggacaagc ggtaggtgtg acgaatttcc
     5041 aaatgatgaa agctttcact tctattgcga ataacggtac aatgattcaa cctcgatata
     5101 tcagcaaagt ggtggatccc caaactggag aagagcgaac aactcaaaca gaagttctag
     5161 ggcaaccttt ttcaaaagaa acaacagaaa aagtccgtga atatatgaga gatgttgtcg
     5221 aaagcgaaaa ctacggtagt gcttatggtg tatatagtgt acctggatac aatgtatcgg
     5281 caaaaaccgg aacagcccaa atcgcttctg atacaggggg atatcaaaca ggagatacag
     5341 cctacctgta ttcgattgtg gagatggttc cttctgaaga tccagactat gtcttgtatc
     5401 taacaatgaa acaccctaag acatatgatc gaatggcatt ggctaaaatc gcaaatccat
     5461 tgatgaaacg ggcaatggat tttaaagaaa ctgaagaaga ttcagataca gaaacaaaaa
     5521 cagaaaaagt atccgtagcc gattatcgta atcttgaagc ggacgttgca gcagctgatg
     5581 ctcaaaaaag tggactgcaa cctgtagtga tcggtaatgg taaaaaagta caaaaacaat
     5641 ctactgccaa cggtgatcag ttgatctctg gagaaaaact gatcctatat actggtggtg
     5701 ataagctgat gcctgacgta acaggatggt caaaagcaga tatcatgaag ctggggaaaa
     5761 tcctcggtat tgaagttagt tttgatggag atggttattg tgtcaaacaa gaacttgctc
     5821 catatgaaaa aatcactgaa gacaaactta gttttacttt agaagaataa gaagagaaat
     5881 aggagagaaa agagcatgga atggacacaa gcattgattc ccattgtcag cagttgtgca
     5941 atgacaattg ccgcaatgcc gctatttatt ggttattttc aaatgaaaaa gcaaggtcag
     6001 gcgattcgtg aggagggacc aaagtggcac aatagcaaag ccggaactcc tacaatgggc
     6061 ggcttagtgt ttctgatcgg ctctattttg acaggaattt gggttggagc ttggcaaaaa
     6121 cagctaactc ctactctttt catcttgctt tttgtattag cgttatacgg agtaatcggc
     6181 tttcttgatg attttataaa aatattcaag aaaagaaaca tgggactgaa ctcgaagcag
     6241 aaattgattg gacagattct agggggaatc atcttttatc ttgtttaccg ttctgaaggt
     6301 tatccaggtg tattgaattt cttcggacta gatctacctt taggaatcat ttatggtatt
     6361 ttcgctctct tctggcttgt tggattttca aatgcagtaa atctgacaga tggcatcgat
     6421 ggattagttg ctggcttagg aagtatctct tttgcagctt acggtctaat tgcttggcat
     6481 cagcatcagt atgatgtatt aatcatttgc ttaagtgttt taggaggttt actgggtttc
     6541 ttcgtctaca acagaaaacc tgccaagatt tttatgggag atgtcggttc attagcgttg
     6601 ggcggacttt tagcggcaat ttctataatg ctaaaccaag aatggacatt gcttttgatt
     6661 ggtttgatct atgttatgga aacagcaagt gtgatgcttc aagttacttc ctttaagctt
     6721 acaggaaaac gaatttttaa aatgtctcca attcatcatc actttgaaat gtgcggatgg
     6781 tcggagtgga agattgatac agttttttgg ttgatcagca ttgtgacttc gttgatcaca
     6841 ttatggatcg tttggtagga gatgacggga aatgaaaaaa atatcaacat atgaaaataa
     6901 gaaagtgctt gtcttaggac tcgcaaaaag cggagtaagt gcggccaaat tattacatga
     6961 attgggcgca cttgtcaccg ttaatgatgg aaaacctttt gacgaaaatc cggaagcaca
     7021 agagctgctt tctttaggga taaaagtaat aacaggtagt catccaatcg aattgttgga
     7081 tgaagaattt tcattgatgg tcaaaaatcc agggattccc tacagtcatc ctttagtagc
     7141 aaaagcccaa gaaatgggta ttccggtaat tacggaagtt gagctagctt acgaagtagc
     7201 ggaatgccca attattggga ttacaggaac gaacggaaaa acaacgacaa caacgatgac
     7261 aggactacta ctgaatgcag gagcagatca aggaattgca cgtttagcgg gtaatattgg
     7321 gtatccagca agtggtgtag cgcaagaagc aaaatcagaa gataaaattg tgatggaatt
     7381 gtctagcttc caactcatgg gaatcacaga tttccgccct catatagcgg tgattaccaa
     7441 tatctatgaa gcacatattg attatcatgg tacaagaaaa gaatatgtca aagctaaatg
     7501 gaaccttcaa aaaaacatga cagaaaaaga ttatctgatc cttaactgga atcagtcaga
     7561 actacaagaa ttagcgcaaa gaacaaaagc acgcgtactg cctttctcga caaaagaagt
     7621 gttggaagac ggtgtgtatg ctgacgatta ctcaatatac tataaaaaag aaaaaataat
     7681 ggaaatatct gaacttggcg taccaggtaa gcataatgta gaaaatgcac tagctgctat
     7741 ttctgtagca aaattgtatg gtatatcaaa tgaagctata cgagaaacgt tgcatttttt
     7801 ccatggcgta ccacatcgta cgcaatatgt aggagaaatc caaggcagaa aattttataa
     7861 tgattcaaaa gcaaccaata ttttagcgac taaaatggct ctaagtggat ttgaaacgtc
     7921 taaggtcgtg ttattggctg gcggattaga cagaggaaat acatttgatg aattaattcc
     7981 ttcattaaaa ggaatcaaag caatggtcgt atttggccaa acaaaagaaa agttgatgga
     8041 tgctggaaaa aaagcaggca tcgagacaat agtaacagcc gattctgtag aacaagcagt
     8101 tcctttagca ttagaaaaca gtacagatgg tgatgtagtt ttattatcac cagcgaatgc
     8161 cagctgggat caatacccaa attttgaaac tagaggaaac agattcatgg aagctgtgaa
     8221 ccgattaaaa tagaaaaagt agaagcgagc gataaaaaat gaaaattttg gttacaggcg
     8281 gaggaacagg gggacatatt tatccagctc ttgcctttgt caattatgta aagactcaag
     8341 aaccgaatgc tgaattcatg tatgtgggag caaaacgagg tctagaaaac aaaatcgtcc
     8401 ctgatacagg aatgcctttc cataccttgg agattcaagg atttaaacga aaaatttcca
     8461 tgcataacat caagacggtc cgattgtttc ttaagagtat ccgtcaagca aaaaaaatcc
     8521 tcagtgattt tcaaccagat atcgttatcg gtactggagg atatgtttcc ggagcggtag
     8581 tgtacgctgc agccaaaatg ggtattccaa cagttatcca tgaacaaaac agtgtaccag
     8641 gtgttaccaa taaattttta agccgatatg tggatcggat cgctctttca tttaaagatg
     8701 cggcttcctt ttttcctgaa aacaaagcag ttcttgtagg taatcctcga gcacaagaag
     8761 tagcagatac taaaaaatca gatattttga aaacatttga tttggatcca gaaaagaaaa
     8821 cagtcttgat ttttggcggt agtcaaggag cacttaaaat caatcaagca gtatcagttt
     8881 ttttaagtga atatattcct tcttcttacc aagttttgta tgcttcagga gatagatatt
     8941 atgaagagat cctaaataaa ataaatcctg aaatacaaaa tattagtgtc cagccttata
     9001 tcaaaaatat ggcagaggtc atggcttcca gtgatctgct ggtggggcgt gctggtgcaa
     9061 cttcgatcgc agaattcaca gctctggggt taccggcagt gttggttccc agcccatatg
     9121 ttacgaatga tcatcagact aaaaatgcaa tgagtcttgt tcacgcagga gcagcaaaaa
     9181 tgattgcaga taatgaacta acaggtgaat cactaagtca gacagtcaac gaaatcatgg
     9241 gtgatgaaga attgcaaaag caaatgtgcc gagcttctaa agaacaagga atccctgatg
     9301 cttcaaaacg tctttatgac ttagtaaaac aaataatcaa aaaataaagg cggtggtctt
     9361 agtgagtaag aaaaaagaac caaaaaaatt gtctcatccg tcttctgatg aaaacaattt
     9421 gactccttgg caaaaggcta accgaaaata tctggcagaa caagagatga aaaaccaaga
     9481 gccaaaaaaa gaagacataa cattagatac acccctttca gattctgaag aagaaaaaac
     9541 ggttcaagag gaacagcaag aagaaagcca gtccgagaaa aaaatcgaat tttttgaaga
     9601 gatcaagcaa aagaaaaacg gtggaccata taacggttct tttttgaacc ggctgcccaa
     9661 tttaaaaagt caacggcaca aagtactatt tcgtcggtta gctttgatca tcactgtatt
     9721 gggtataccg ctgatttttt tgatctatta tgtttctcct tatagcaaat tacaagcagt
     9781 tgcagtatca ggcaataaga cagtcaacag ccaagaaatc atctctgata caaaattgtc
     9841 attaggagaa aatgtttggg gacagtattt tcatcgatct acttatatcg atcgccttaa
     9901 aaaagcacag ccaagaatcg aaacagcaaa tatacatttc aaagggatga atgaattcga
     9961 tttggatgta acagaatata aagagattgc tttgatcgca aaaaacaatc aatactaccc
    10021 agtgattgaa aatggaacag tattagatga aaaagtagca aatcctacaa aaaatctccc
    10081 tatcttggaa gagttcaaag actctgcaaa aatcaaagaa ttaacaaagc aatacaatca
    10141 gttatcatca gaattacaaa aagcaatttc tgaaatcaag tacacgccaa gagcaagcaa
    10201 taaaaatctg atccaattga atatgaatga cggcaatcaa gttattgtaa atatcaataa
    10261 tcttgcgaac caaatgaaat attattcgca agtagccaaa gatatggatg aaaaaggtgt
    10321 gatcgacatg gaagttggta tcttctcgca tccttacggt agcactcaag aaacggaaga
    10381 atcgggccaa tcggaagaaa caactgaaag tgaagaaaat gcggatcaaa caaacgaatc
    10441 aagtgaagag gcagaaacgc aaaaagcaca aacacctgga tcgtctagca caaacggatc
    10501 atctgattca aatgattcaa gtgaaacaag tcaaatagaa gataatccaa gtacaactcc
    10561 gtcaagttta aatgaaaatt gataaaacaa ctttaaaaag taagaatgta ctttctaaaa
    10621 accagtaatt tatggtagaa ttggaagcag tgaatatatc tattaataag tatattttaa
    10681 aacggacgct agttgttcgc ttgtatatgg aatagtaagt aggagggaat cccattcatg
    10741 gcaaaaacag gaatgtatgt aggccttgat attggaacga catctgttaa agttgtcgtg
    10801 gctgaatata tcgacagcca aatgaatatt attggagtag gaaacgcaaa atcagaaggg
    10861 attaaccgag gcattatcgt tgatatcgat aaaacggtcc aagcgataca acgagcagta
    10921 cgacaagcag aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg tttgccagca
    10981 aacatgcttg aagtagaaaa ttgccaggga atgattgcag ttaacggaga ttcaaaggaa
    11041 atcaccgatg aagatgtcag aaatgtggct tcagctgccc ttgttcgttc aatacctcct
    11101 gaacgtcaaa tcgtgtcaat attgccacaa gattttactg tcgatgggtt cgagggaatc
    11161 aaagatcctc gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt gtttacagga
    11221 cctaaaacga tcatccataa catacgtaaa tgcgtggaaa atgccggatt aatcgtcaac
    11281 gaaatggtga ttactccttt agcgctgact gaatcaattc tttctgacgg agaaaaagat
    11341 tttggtacga tcgtcattga tatgggtggc ggccaaacaa caacggctgt tatgcatgat
    11401 aagcaattga agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac taaagatatc
    11461 tctattgtat taaatacgtc atttaacaat gcagaagcgt tgaaaataaa ctacggggat
    11521 gcatacccag aacggacatc accagatgaa gaatttccag tagatgtgat tggtcagtca
    11581 gaaccagtga aagtcgatga acgctacttg tcagaaatta tttcagctcg aatggaacag
    11641 attttcaaca aagcaaaaga tgcattggac caaatcgaag cattagagtt gccaggtggc
    11701 gtcgtattga caggcggagc tgctagtctt cctggtgttg tggacttggc acaagaaatc
    11761 ttcggcgtaa atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa cccggtattt
    11821 acaaatgtaa taagtatcgt ggattattca gcaaacttga gtgaagtgta tcaattggca
    11881 aaaattgctg ttacgggtga aacctcagca gctcgtcaaa tggtagttga acaagaaaca
    11941 acaaacacat atgagagcta tgaagcacct gaagaaacaa tctacgatga gccggaacca
    12001 aaagagtccg gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa tatttttgat
    12061 taattcatcc tgggaggaat aagatatgga attctcaatt gataataata tcaatgacgg
    12121 cgcagtaatc aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa accgaatgat
    12181 tgaagaaaac gttaaaggtg ttgaattcat tacagccaac acagacgtgc aagcattaaa
    12241 aaattcaaaa gctgaaacag ttatccaatt aggccccaaa tatactcgtg gtttaggtgc
    12301 tggttctcag ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat ccttacgcga
    12361 agctttagac ggtgcagata tgattttcat cactgcagga atgggtggag gaaccggtac
    12421 aggtgctgca ccaatcgttg caggtatcgc gaaagaattg ggcgcattga ctgttggtgt
    12481 cgtgacacgt ccattcactt ttgaaggacc gaaacgtggc cgttttgctg cagaaggtat
    12541 cgctcgtttg aaagaaaatg tggatacatt attaattatc tcaaataacc gtttattaga
    12601 agttgtagat aaaaagacac cgatgcttga agctttccgt gaagcagata atgttttacg
    12661 acaaggtgta caaggtatct ctgatttgat cacagcgcca ggttacgtta accttgactt
    12721 tgctgacgtg aaaacagtga tggaaaacca aggtacagca ttgatgggga tcggtgtggc
    12781 tagtggagaa gatcgcgtag tagaagcaac gaaaaaagca atctcctctc cattgcttga
    12841 aacatctatt gatggagctg aacaagtact tcttaacatc acaggcggtt tggatatgac
    12901 attgttcgaa gcacaagatg cttcagatat cgtagcaaat gcagcaactg gcgatgtaaa
    12961 tatcattcta ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg tgacggtcat
    13021 cgcaacaggt atcgatgaat caaaaaagga acgcaagtct agccgaccag ctagacaagc
    13081 acaaatgcaa tcacctgctc aaaagactgt attagatatg gatcaagcta aaccaacttc
    13141 ttcagaagaa gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa atgttcgtcc
    13201 tagagttgat gattccaatt ttgacaatat cgaaaaaaaa gaatttgata cattcaaccg
    13261 cgaagaaaca aaatctcatg gcgatgatga attaaatacg ccaccgtttt tccgtcgtaa
    13321 aaaataaagg agaggaagag cacatgattg ctgacaactt gcagaaaatc aagcaagaaa
    13381 ttcaggattc gtgtgctctt gttcatcgtg atccgaaaga agttacatta gtagctgtaa
    13441 caaaatctgt tgaatccgct caagctgaag aattggttag agctggcgtt atgaattgtg
    13501 cagaaaaccg agtagataaa ttattagaaa agaaacaaga tttatttgat tttcctgaaa
    13561 taaagtggca tttaattggt aatttacagc gcagaaaggt aaaattgatt gtaaatgaaa
    13621 ttgattactt tcatgcgttg gacagtttaa gattagcaga agaaatccag aagcggttag
    13681 agaaagaatt atcctgtttt attgaagtaa atgtttctgg tgaggaatca aaacatggaa
    13741 tttcaccaga tgagctttta ccgtttgtgg aatctttagc tgcctgcaca aaaatcaagg
    13801 ttgttgggtt gatgactatg gcgcctaaag aagcttctga tgagcaaatc cataaagtgt
    13861 ttggtacgct gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc catgcaccat
    13921 gtagtgaatt aagtatgggg atgagtcaag actttcaaat tgcgattgaa gaaggtgcaa
    13981 catttatccg tgtaggaaca gcgctattca aagacgaata aaggaggaac aaaaatgccg
    14041 cttttaaaca gaggagcaat tgctaatttt ttcggattag ctgatgagga agagtacgaa
    14101 taccaagatt atcctgaaac aacaaaaaag gctgtacagc caactaaaaa aaatacaaca
    14161 cagaatcaat caactcattc tgtatctgct gcgaatactc atgaaaaaca aaaaacggcg
    14221 acttcacgtc ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa agtaattgaa
    14281 ctacatcaat cacctacaaa aactgtagcg aaagaaagaa cagcttcaag aacctctaca
    14341 caacctaaag caaatcttgc tggaaaaatc actgtaatgg aaccaagagc ttattctgaa
    14401 gctatggcga ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa ttttcattta
    14461 gtagaagaaa aacaagcgag aagaatcgtt gattttctaa caggaacagt ttatgcacta
    14521 gatggagata tcaaacgtgt tggagatgag atttttcttt gtacaccgac tgggatggag
    14581 attgacagtt caaccgctca atcttttgca gatgcgaatt tgtttgatct gtaagaggag
    14641 gaactacgat ttatactatt gcactattac ttcatttact tcaacgggca gcctatttct
    14701 atagcattat cttgatggta tatgcattgc tctcttggtt tccaggcggg tatcaatcag
    14761 ggctaggacg attattagct aagatctgcg aaccgtatat cagtttgttc gatcgtctgc
    14821 ctttgaggat tggacccttg gatttcacaa ttttaggggc aatcctgatc ttgaacttag
    14881 ccgtacaagg tttagcacag atcgttatat ggataactgt ccatttagtt tattgattga
    14941 agcaacaaaa tctttattct cagaatagat aggaggggaa aattttggat gcaaatgtat
    15001 accagcactt tcgggcagat gaacgtacat ttatcgattc ggtaggcgat tggattgaac
    15061 aggttcaagg acaatatgca ccctatctga ccgaatttct tgacccaaga caatcctata
    15121 ttttagaaac actgattcga caaagcagtg atttgcgttt catgttttat ggcggatatg
    15181 aacaggcaga aagaaaaaga tgtttgattt tccccgatta ttacgaacct gtagaggaag
    15241 attttgaaac agcattgtat gaaatccatt atcctagcaa atttgccagc ctaagtcatg
    15301 ggaaaatcct tggaacttta gtgggaacgg ggatcaaacg ctcttacttt ggcgatatca
    15361 tctcggacgg tgatcgctgg caagtattta ttgcaaatga ggtggatcat tttgttgctg
    15421 cacaagtaac aaagattgga aaggttgctg tacgcttgga accaatcaaa tacacagaaa
    15481 tgattatgcc gaaagatagc tggacacaag aaagaggaac agttagttcc atgcgactag
    15541 acagtgtgat ctctgagatt tataatattt ccagacagcg gtccaagcag ctgatcgaag
    15601 ctggtaaagt aaaagtgaac tggacagaaa atacaagacc tgattttgta cttgaactat
    15661 tagatattat ttcagtcaga ggttatggca gagtccagat ccaagaactt gaggggaaga
    15721 cgaaaaaaga aaagtacagg gtactgttcg gtgtattacg taaataaaat aaaaaggtgg
    15781 tttactcatg gctttaactc cattagatat ccaaaataag acattcccga caaaaatgcg
    15841 gggatataat caagacgaag tagatgattt cttagattta gttgttcgtg actatgagga
    15901 attgactcaa agaaatcgag aattagaaaa agcagtcaaa cattctgaag aaaaacttga
    15961 gtacttcaat gaattaaaag atgctttgaa ccaatcaatc atcgtagctc aagatacggc
    16021 tgataaagta aaaacaagtg caagcaagga atccgaagtg atcgttactt ctgcacaaaa
    16081 caaagcagat gagttagttg cgaacgctga aaaacgtgca catcaattaa caactgatgc
    16141 agaagaaaaa gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac agcttgcgac
    16201 agaaactgaa gatcttaaga aaaaaacacg agtattccat caacgtatca gcttgatgct
    16261 tgagtctcaa ttagaacaag tgaaaagtcc ggaatgggat gaaattttac aaccattctc
    16321 cagctacgtg actgacagcc atgaagtgat caaagaagtt ttagcaaaac agcttgacaa
    16381 tgaaaatgat actgatgtaa actcagacac aggagtcata gaagcaccta tgacggcatt
    16441 tgatacacag gctattgata tctcgatcgt gcctggtgaa aatgaaaacg agtagaacag
    16501 aaaagcactt tgttattctt aaagcgagcc gatgagagtg gaagttcggt aagacactgc
    16561 atggtgaaag atcctctact tgagacctgc ttttccgaaa ttagagtaag aagcagcggc
    16621 tgacctcgtt attggtcaca gagagtttgc actagataga tgcaactaaa tttgggtggt
    16681 accacggcat gttcgtccct tgcggctgaa catgcttttt tgtgtccgga aaacaaataa
    16741 aggagtgtat ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa caaaattccc
    16801 tatgcgtggt aatttgccaa accgagaagg cgagtggcaa aaagaatggg aagaaaatcg
    16861 aatctaccaa aaaagacaag agttgaacga aggaaaacct agctttgttc ttcatgatgg
    16921 acctccatat gccaatggta atatccattt aggacattca ttgaataaaa tcagtaaaga
    16981 cattatcatt cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc caggatggga
    17041 tacacatgga ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac gtaaagaaat
    17101 gtccatggca gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag tagataagca
    17161 acgagcagat ttcaaacgcc taggagttgc aggagattgg gaacatcctt atgttacatt
    17221 agatccttct tatgaagctg cacaagtacg cgtatttggt aaaatggctg aaaaaagcta
    17281 tatttacaaa ggattgaaac caatttactg gtctccgtca agtgagtctt ctttagcgga
    17341 agctgaaatt gaatataaag atgtaaaatc tccatctatt tttgtagctt tcaatgtcaa
    17401 agacggcaaa gatattcttg atgaagatac agcttttgtc atttggacga caacaccatg
    17461 gacattgcca gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg tattggtgga
    17521 agccgatggc cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc aatcagaaat
    17581 cggttgggaa aatgtaacag ttctaaaaga atttgctgga caagaaatgg aatatatgac
    17641 tgcaagtcat ccattctatg aacgtgaatc acttgtgatt ttaggcgatc atgttacttt
    17701 agacgctggt actggattag tccatactgc tccaggacat ggggaagatg actatatcgc
    17761 tggtagcaaa tataaactat ctgtagtgtc acctgtagac tcaaaaggat tgtttacaga
    17821 agaagcacct ggatttgaag gtatctttta tgacaaagca aatcctatga tcactgatct
    17881 attaaaagaa aaaggagctt tgctgaagct agatttcttt actcatagtt atcctcacga
    17941 ttggcggacg aaaaagcctg tgatctatcg ggcaactcca caatggtttg catctatcga
    18001 taaattccgt cagaatattt tggatgaagt agaaaaagta gagtgggtta tcccatgggg
    18061 taaaacacgt ttgtataata tgatccgtga ccgcggcgat tgggtaattt cccgtcagcg
    18121 tgcatgggga gtaccacttc cgattttcta tgctgaaaat ggagaagcaa tcatcacacc
    18181 agaaacaatc gaacatgtgg caaaattatt tgctgaacat ggatcaatta tctggtttga
    18241 aaaagaagcg aaagaacttc ttcctgaagg atttacccac ccaggttctc ctaatggcga
    18301 atttacaaaa gaaaaagata tcatggatgt ctggtttgat tctggttctt ctcatgaagc
    18361 tgttttacgt cagcgaccag aattatcatt cccagcagat atgtatttag aaggatcaga
    18421 ccaatatcgt ggctggttca actcaagtat cacgactagt gtggctatca atggtgtagc
    18481 accatacaaa tcagttattt ctcaaggatt tgctttagat ggtgaaggcc gtaagatgag
    18541 taaatcttta ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg gtgcagatat
    18601 ccttcgctta tgggtatcta gtgtagatta tgaagcagat gttcgtgttt ctatggatat
    18661 cttaagtcaa gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt tcttattagc
    18721 gaatacagaa gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt tacgttctgt
    18781 agacaaatat atgaccgttc gattgaacca agtaatcaaa gaaatccgag aagaagggta
    18841 cgacaaatat aacttcatgc atatttaccg tacagttatg aacttcttga cggttgatct
    18901 atcgtcattc tacctggatt ttgctaaaga tgtagtttat attgaagcag aagatgatta
    18961 tcaacgtcgt tgcatgcaaa ctgttttcta tcaaacagct gtagctttga caaaattatt
    19021 gactcctatc attccgcata cagcagaaga aatctggtca ttcctgaaag aagaagagga
    19081 gtacgttcag ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag aagaattgat
    19141 ggatacctgg actgctttca tggacttccg tgataatgtc ttaaaagcat tggaagaagc
    19201 aagaaacagc aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt atccaaaaca
    19261 acaagttcgt gaaatgttaa aagctttgga tgcagatatt gcacaattat tgatcgtttc
    19321 accagactac tttgaagtta tggcagcaga tgaacaagtg ccagataacg ctatggtatt
    19381 tgatgatgtt gctattacag tggaaaaagc agacggggaa acttgcgatc gctgtcgcca
    19441 agttcgtaaa gatgtaggag tagatgaaaa acttcctcat ctatgcggtc gttgtgcaaa
    19501 aatcgttgaa gacttctatc ctgaagctgt agctgaagga ttcgaagaaa agtaaaaggg
    19561 aaaagggggt gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg tttaattttc
    19621 cacgttcacg ataccagtta tctggttgcc agatccattc gaatccgtct ttttcaagta
    19681 gttcgaaagc tgctttaggt cccattgatc cagctttgta gttgggaaaa tccggttgtg
    19741 tttggtccca agcttttcga atgacatcca cgatatgcca agattgagcg acttcttccc
    19801 aatgagagaa gttcgttcca tcaccattca acgcatctaa tagtaatttt tcgtaagctt
    19861 ctgggctatt ttcaaccatt tctgcactat ttctaaattc caatttgatt ggttccgttt
    19921 caaatccttg ccctacttct ttcccattca aggataaaga aaatccttca gttggctgaa
    19981 tatagatcgt tagaacatta ggtggaagtg tcgtatcgtc acatggttca tcgacagatg
    20041 ttttgaatac attaacagga acttgtttga atacgatgtt gatccgagtc cctttttcag
    20101 tcaaacgttt gcctgtacgt acatagaaag gaacacctga ccaacggaag ttatcgatag
    20161 tgaatttgcc tgcgacgaat gtttcagtct ctgattgagc atccactttg tcttcttcgc
    20221 gatagccctt gaattgtaca ccatccaatt caccttgagc atattgcccg cgaacgaagt
    20281 tttctaacac ttcttcttcg ttataaatgc ggatagcttt caatgctttg atcttttctg
    20341 tacggatttc tttttcggaa aatgctactg gcggttccat tgcaagtaat gctacaacct
    20401 gtaaaatatg attttgcacc atgtctttaa gtgcgccact atgatcgtag tagcccccgc
    20461 gatcttctac accgaggttt tcgccaaaag taatttggat attgtcgata tagcgattgt
    20521 tccattgagc ctcaaaaata ttattggcaa aacggatagc tgagatgttt tggatcattt
    20581 cttttcctaa ataatgatca atcctgaaaa tatcttgttc tgggaatact tctctgattt
    20641 gctggttcaa ttcaaaagca gactggtaat cagaaccgaa cggtttttct atgatcaaac
    20701 gattgaagcc attttttgtc ataatatttt gtgatttcaa atgagaaacg atcgttccaa
    20761 aaaattgagg tgccatagcc aaataataga cacggttacc gtttaattga tagtgtccat
    20821 ctaatcggtc agacaattct tttaatgtct gataatgcgt tgaatcattt acatcatggg
    20881 attgatagta aaaatggctt gcaaaggtag ttgcttcttc atctgaagga ttcaatgcac
    20941 ggattgtttc tttgacaact tcacggtagt gctcatcgct ccagggacgg cgagcggttc
    21001 cgattacagc aaaatgctcg cctaaatttc cttttctgta aaggcggaac aaagacgggt
    21061 acaatttacg ctgggctagg tcacctgtac cgccgaatat cgtaaacaat acattttttt
    21121 cattagacat gtggatacac tcctttatat tttatcgaca aaaatcgtgc tggccgcctt
    21181 aaagctgacg gggattttct ttctatcatt ttggatagtg atcggtcctt cataagctgc
    21241 gatgtcaacg atctcatatt cttcgttgat attcaggtca atagtgacta agtaatccaa
    21301 caattctttt tcatcaagca ctcgtgcgat acgcactttt gtaccgacag gataggactc
    21361 caatgtttgt cggcgttctt catggacaac ttcctctttt ttaggaatcg atcctccatg
    21421 aggacaatgt tcaggatgat tcaaatagtt ttctaaatga tctgcaagat gttccgaagt
    21481 cacatgttcc aatacttcag catcatcatg gacttcattc cagctatagt ctaaatgttc
    21541 aactaaaaaa acttcccata agcgatgttt gcgaatcaga ctgctagctt tttgcagtcc
    21601 gcttgcagtc aattgtacac cttgataagg ggaatgttct acaagctctt ctttcacaag
    21661 tttagaaatc atttcactga cagatgcagc tgagacatct agactagata cgatttgttt
    21721 attgttgact ttcgttgtat ctccacctag ttctaaaatg attttcaaat aatcttctcg
    21781 atttggtgtc atattcgatc atcctttcat ggtaaaaaac catcattagt aagtgtaaca
    21841 aaaaaaacgt taattgacta tttttctaaa taaaaaacaa aggattttta gacgttttta
    21901 caacaaaaaa cgaaagatga tagagaagac tcattctttc gtttataaag gatatatata
    21961 tacattggga gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc
    22021 ataaagagtt tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac
    22081 taaacgatat tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa
    22141 cgtgtaatca gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt
    22201 accttgatac tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat
    22261 aaaacttgag agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa
    22321 atcactcctt tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta
    22381 ccgaataaaa gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca
    22441 aattatgata ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg
    22501 aaatagcttt ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg
    22561 gagaaataat tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag
    22621 gcgctgaagt ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga
    22681 ttgctgtaaa aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg
    22741 caattgatgg tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg
    22801 tatacaagcg tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg
    22861 tcggacaaaa agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg
    22921 cttttcctga tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg
    22981 cacaagatgg ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg
    23041 caacgatcca tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa
    23101 gcggagttca aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc
    23161 aggccattcc atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta
    23221 aaataatgat caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag
    23281 cagctttaag cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag
    23341 cacaggaatt tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt
    23401 ttttcaatat tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta
    23461 gtcaagctaa gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt
    23521 cgacgccaaa agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt
    23581 ttgaagcagc aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat
    23641 taaaagacga gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc
    23701 aagtattaga tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga
    23761 tggatttagg tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag
    23821 tccattattt cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac
    23881 gttatgagaa ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt
    23941 cagagattta ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag
    24001 cacaatttca tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa
    24061 atccaatggc agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag
    24121 cttggggatt gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt
    24181 atgatttaag aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat
    24241 gaacgaattt gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt
    24301 tatctcacct tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat
    24361 agtagcggtt tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact
    24421 aaacgtggaa ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc
    24481 tattcaagta gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc
    24541 tttggtgggc ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat
    24601 cttgattggt acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca
    24661 tcaagtgaaa acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca
    24721 aatcattgat caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc
    24781 gacagcagat cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat
    24841 tgacgtgaca gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc
    24901 gatacgaaag aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct
    24961 gattttcttt aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg
    25021 tgtaccggta ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga
    25081 acaattcaaa aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt
    25141 agatattaca ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta
    25201 tttgcatcgt tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt
    25261 acaagataat aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga
    25321 agagattttt ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc
    25381 tcctgcagta aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata
    25441 taacaaagaa aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg
    25501 agcaaggcgc aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt
    25561 tgccagagga ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa
    25621 aaccaacaag gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat
    25681 gcaagccgat atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga
    25741 ccagtcgttt actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct
    25801 tctacgcaag tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata
    25861 cagcaggcaa aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga
    25921 gaaactggta cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca
    25981 gagatgcttt ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat
    26041 ccgcaactgc tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct
    26101 caagatagtc ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc
    26161 caccgtttac cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac
    26221 cgtccgcttg gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg
    26281 actgaatctt cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc
    26341 ttgccgaatc ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc
    26401 aaacaagaag cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc
    26461 ttcatgcttt atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc
    26521 tgtgcaaaat cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa
    26581 gaagaacttt cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt
    26641 ctcgaccgat tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat
    26701 gttgtctggt cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa
    26761 gtcacttctc tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa
    26821 gatgtggatg cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa
    26881 gatcttttgc cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa
    26941 cggttagacc gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt
    27001 acattggatc gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg
    27061 aaaaatttta agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa
    27121 gaacaagttg agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat
    27181 ctaggagaaa gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc
    27241 agacagactg cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg
    27301 aaagctttta atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa
    27361 tatgtcaaaa actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga
    27421 tcattaaaca ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc
    27481 acgatgacaa gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc
    27541 ttagaagata tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc
    27601 aaagcggaaa agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact
    27661 ggtgaaggag tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag
    27721 gtagaactga tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc
    27781 ttattggaag aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat
    27841 atcccatttt tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt
    27901 atagctgatg atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc
    27961 gatgtctttt ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag
    28021 accgatttgt cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc
    28081 ttcatgttag acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt
    28141 ttctcaaaaa tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa
    28201 cgaaaatatc aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt
    28261 gaaaataaaa taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt
    28321 gcgcactata aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg
    28381 gcatgcttct tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa
    28441 aaaaacaatc atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat
    28501 gcaaaaagca gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga
    28561 tgcagataat aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg
    28621 tttcatgaaa gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat
    28681 caatatgcaa gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct
    28741 aatgaaataa atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta
    28801 gaagccatca tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg
    28861 attgcggctg ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa
    28921 gaatcactta tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga
    28981 aatcatatgc aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt
    29041 gcgtttactg atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa
    29101 atacaacttc ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta
    29161 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    29221 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    29281 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    29341 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    29401 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    29461 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    29521 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    29581 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    29641 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    29701 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    29761 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    29821 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    29881 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    29941 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    30001 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    30061 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    30121 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    30181 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    30241 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    30301 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    30361 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    30421 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    30481 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    30541 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    30601 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    30661 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    30721 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    30781 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    30841 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    30901 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    30961 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    31021 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    31081 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    31141 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    31201 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    31261 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    31321 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    31381 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    31441 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    31501 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    31561 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    31621 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    31681 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    31741 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    31801 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    31861 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    31921 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    31981 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    32041 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    32101 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    32161 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    32221 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    32281 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    32341 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    32401 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    32461 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    32521 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    32581 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    32641 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    32701 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    32761 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    32821 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    32881 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    32941 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    33001 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    33061 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    33121 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    33181 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    33241 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    33301 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    33361 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    33421 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    33481 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    33541 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    33601 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    33661 atatttgctg acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt
    33721 atgagggttt ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt
    33781 taagagatca atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt
    33841 ttttccccat tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc
    33901 gaaatcgata tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc
    33961 agcaactaac acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt
    34021 cattaaagat ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc
    34081 ttttcctttg attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc
    34141 atctttcaac gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc
    34201 tttagcatac ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac
    34261 cgctggcata tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg
    34321 agatactgtt ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag
    34381 atttttgcga tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc
    34441 ttttgcttct ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc
    34501 ttttttgtat gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat
    34561 tgaatcagct ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt
    34621 tattattttt tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta
    34681 atggtataat tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg
    34741 atttttaaaa gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc
    34801 tggtttttta ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag
    34861 taagagttga gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt
    34921 tgccgacatc acctaatact atggagctaa tcgttttttc taacagacca aaatcagata
    34981 atctatttaa gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag
    35041 tgaaattttc gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta
    35101 attgattcag tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa
    35161 gtaaacaatt cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt
    35221 ctacctcgtc atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga
    35281 aaacatcttc tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga
    35341 atttctcgat gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa
    35401 cgccaccaat ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt
    35461 ttttggttag tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta
    35521 caagcattgt gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta
    35581 actcttagtg gggcgtggtt catttatttt ttacttactt tactattaat catatcccat
    35641 tgagcatagg atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact
    35701 ttatcgccct ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa
    35761 actttaggtg ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata
    35821 atggctattc tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca
    35881 ctaatatatt tagtaaaagc tgtattttgc tcagtagatg gagtaataga ataataacta
    35941 tctaagaaag caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg
    36001 cttttattgc tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg
    36061 ttattgtcat ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc
    36121 ccgataataa ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt
    36181 aacatgatta aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt
    36241 aatagatcct ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta
    36301 aaatcgaatt cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt
    36361 tcatctgcac ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc
    36421 tctatatatt tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg
    36481 ttttcatctt tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta
    36541 aattgtatat aaccattact catacctggt ttttttaatt gtacggctga tatattttta
    36601 aaggggatag ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta
    36661 ataattgaaa tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa
    36721 atctcttttt ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca
    36781 tatatgagga aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt
    36841 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    36901 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    36961 tccataaatt tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata
    37021 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    37081 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
    37141 tgagataaat gaaaggcttc tgcagtttca ccgtatagat tttcattcaa aaaaatgatt
    37201 ctgggttttg gatagtagaa accaggctct ttcatctcca tataaactac tttcaaattg
    37261 tattcgctca acatttcttt caatttcaaa tacatacaaa ccatcactcc aactattcat
    37321 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    37381 ttccatcaaa agaaaaaaca gtatcgtctt ctgataaatc cacatgttta ggggtttctc
    37441 tttcttctct acctagaagg tagtctacag agacatcgaa atagtcagca atccgactta
    37501 attcatctga attaggagta ttatttttcc actttgctag ataaccgtta gaatatccaa
    37561 aattaatttc taactgccgt atagatatct ttttcctctt cgccaagtct tttattattt
    37621 cataggtatt catcgataaa acaacctttc taaatgttta caaaaaaagt ttagaaaaat
    37681 acgcagaaat aatttgacta attctgagta atgagctata ctatatcttg taaacagatt
    37741 taacaactaa aaagacaaca aaaaacacta ttgatatgta aatgcagacc gccaagaaag
    37801 ctttaaaatc aatgttttta tgtcttattt aattatgctc ttagtataga gtattactca
    37861 gatactgtca attgaattta gaaaaaagtt gttaaatttg tttacgaata taaaagaaag
    37921 gaatgagaaa tatgaacaca ccgcaaattt ttaatttcga acaaaacgaa gttcggacaa
    37981 ttttagtaaa tgatgaacca tattttgtag gcaaagacgt tgcgagtgtt ttgggttatt
    38041 caaacactaa agatgctttg tcgcggcatg tagatttgga agataagatg gggtcgcgaa
    38101 tcacgacctc aggtcaatca agagagatga caatcatcaa cgaatctggt ttgtacagtt
    38161 taatcttaaa atcaaaactt ccctctgcca aaaaatttaa acgttgggta acaagtgaag
    38221 tgttgccagc aattagaaaa catggaggtt atctaactcc agaaaaagta gaagaagctt
    38281 tgcttaatcc agatacaatc attcaattag caactcaact aaaagaagaa agaactggaa
    38341 gattaatcgc agaacaaaag attgcagagt acgaacctaa aatctcctat ttagatagca
    38401 tattatcttc tacagattca gtaacaatta gtcagattgc agcagattat gggatgtctc
    38461 cacaacagat gaataaatta cttcataaac taggtattca gaaaaaagtc ggtaaccaat
    38521 ggttattgtg caaaaaacac atgaaccaag gatacacaaa atctcataca actgagatcc
    38581 cgaaagccga tggtggcact aaaattgtaa tgaataccaa atggacacag aaagggcgtc
    38641 tatttatcta cgaattacta aaaaaagaag gatattaccc tcaaatggat ttagaggaaa
    38701 ttggttagaa aggagtttta gtatgactga cattgcagaa atcactcaac gagatagaga
    38761 aaaaatcaaa gaatatgtcg aaagttcaaa gttcttaact tacaccatgc ttgctgaaag
    38821 atttggaatt agcaaaagct acttatcttt aattttaaac ggtaaaaaga cttctgcaga
    38881 agcaaacaga attatagatt cgattatcac tatgtacgaa ttgtaaaggg aggaacagct
    38941 aatgcaatat ctagaagcaa aaatcccaat tccagaaggc tatgtaatta tctcccaagt
    39001 ggattatgag gagttaaaaa aagctgatga tactggtaga tggatgacgt tgccagaagt
    39061 gctagaacgg attaacagaa aatatgattg gtttacttct agagttttaa agaacccaag
    39121 atatagaaat attatcgata tcgagaaaaa caaaaatgga tttgtctatt atccagttga
    39181 aggaagagac acatatctat ttttaagaag taaaacactt gaatttttag aaacaaattt
    39241 ttcggaaatc ttaaggaggc aagcggatgg gaaaatttaa tagagcgcta gtgttcagcg
    39301 caccgctaat catctacgct ttagaacttt ggggaagcag gcaagcgttg ataggaacga
    39361 ttgtttacat ggtttggatt tttatggggc ttgatgaagc tgaagctgag tacagagcga
    39421 aaaagccaac cgagggggct gactaaaaat gaatagaaaa gagaaactag aatgtatatt
    39481 actattactc agtttaattc tttcactaat ttctctattg ggaagtttgt atttttgata
    39541 tcaacaattt tgtggatttg ggttctattt agagtaaata tcatttttgt ttggtgatta
    39601 aggagtttat taaaattaat tttccctact tcaaatgcaa ggtaacaaga tatagcactt
    39661 ttaggaggaa tatttagcgg tacagcttca gagtatattc tattatcatc gactgatcct
    39721 cctttagcaa ttaatttctt aaatggagta gctgaccatc tgcggtttaa gttagcggat
    39781 gaaagttcta aattaactaa catagctggt tctgttgaat agttagaaat gattactttt
    39841 gtataaatca tatctgcagc gaagtaagaa gcattaagtt caacttctag ttgagggcgc
    39901 attcctttta tttttatccc tgttaaaaac gtactcaata taaaacttat tattgacatc
    39961 cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt ttaactaaat
    40021 tataccaaaa aggagagaag aaataatgca agaattagta attttgaaaa ataaagaagc
    40081 tgtgactacg agcttacaag tggcagaaag cttcgagaaa aaacatcaac atgttttaag
    40141 agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttg tagaaggtaa
    40201 tgaaccagat tcatatggca gaaatcgacg agttttcttc attagtagag atggattttt
    40261 cttgctggct atggggttta caggaaagaa agctatctac ttcaaacaaa aatacattga
    40321 agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga
    40381 tatgatgatc tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa
    40441 agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa
    40501 agttatggga gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt
    40561 attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt
    40621 acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttgc cagaaactgc
    40681 gatccgtatg gaaattgatc aactgaacag acaacagaga ctttttggtg atgacaatga
    40741 atagagctga agcgctaaga atagggacgg caattgctaa ttgctggtgg aaatactata
    40801 aaccaatcat cctaagtcaa caacatattg acaagcaaaa agcatggcaa caaataaaaa
    40861 agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca
    40921 tatggaaaaa gaactttcca ctctagatca atatttgatt gatcctgatt ggggcaagcc
    40981 gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga atgaagaact
    41041 agcttgggat caagatgatt taggcaacca tgtaactatt tgggatcatg tttatcttat
    41101 ccatctatcg aagcattcga ataaacctga atatatttac gtcatcgaag atggcttgat
    41161 tgatgcgcta gaagagtacg acagagataa cttgattgat atctcttatt acggaccagg
    41221 taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaatcaaa gggacaacga
    41281 actttgaaaa actttttagt cgtaagttaa ataaaattct caagaaaaaa ggaaattttg
    41341 attatttatc ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg
    41401 taactattaa tgagtacaaa cactacaggg ttgtttctgg aactcatcaa gactttcttg
    41461 ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct gtctcagtaa
    41521 cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga aaccaaccag
    41581 ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt gtgaaagcat
    41641 tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca acaccaccta
    41701 gaatcgatac aaagaaatta aacatgctag agacgattct agaagctttc aatgagcaga
    41761 tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca gataaattag
    41821 ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa tgtgccttga
    41881 tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat gagtgtttaa
    41941 accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag acataagcgc
    42001 aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg gctacgatgg
    42061 tgatcgagtc attgaaattc gttttattga tcctagacgt ttcaccgtac agcaacgaaa
    42121 cttcatctat gcgctgatag gcgatatttt tatcgataca ggcatgccaa cggacttctg
    42181 gaaggaattc ttctactttc gttttgaagg tgtcacaggg cgcaaaataa gcctgaaaga
    42241 cgaatcgaat acaactgtga gtgatgccaa tgtcttagca aatatcatct tagatttcat
    42301 ctttgaacat catattcctt tcaaagaagg ctatgagatt ttacctgcga atcaagaata
    42361 ttacttctac aaatgcatca caaaaagagt ctgctgcatc tgtggcaaaa caggagctga
    42421 catcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcatgcaga
    42481 gtacacattt gcagcactct gcagaatcca tcacacagag aagcacaaaa taggtgtgat
    42541 taatttcaaa aataaatatc aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa
    42601 gttaaatatt ggagggtaaa aatgacagaa catcgaagtt attatgcgat tataccagcc
    42661 aacgtaaggt acgacaaaag acttaaacca aatactaagt tgttatacgg agagataacg
    42721 gccttgtgta atgaaagagg cttttgttgg gcaggcaatg agtactttgc agatttatat
    42781 ggtgtgaata aagagaccat atcgcgatgg gtaagtgatt tgattaagtt tggatacttg
    42841 aatcgggaaa tcatttacaa agagggtacc aatcaaataa tcaataggta cctacgaatt
    42901 aatcaatacc ctattgacga aaaacgcaat acccctattg acgaaaaagt caaagataat
    42961 aatacatcta ttaataatac atttaataat acaaaagaat atataagaga gttaccgcct
    43021 tcgaaaaaat cgaaggctaa gcccgtccgt cataaatacg gagagtataa aaatgttctt
    43081 ttgtcagatg agcagatgga gaaactcaaa acagaattcc ctaatgatta ccaagagcga
    43141 atcgaacgac tgtcagagta ttgtgaatca tctggtaaga cttataaaaa ctatttggca
    43201 actattcgaa gttgggcaag gaaagaaaaa agtgaaccta agaacgcaag cagtggatac
    43261 aagcgcacag gaagacgaga gaagcttcct gaatgggcaa tcgaccaaga agcctatctc
    43321 aagaaaaaag cgctagaacg agctaataga caatcaaaag caccattcta agaggtggaa
    43381 aaatgaagat cgattatcta gaactaatta atgaaatagc gaattataaa aagggcgagg
    43441 aattagacgt cctgagagac gtatatgatc aactcgaaga agctggaatt gaaggaatta
    43501 agaatgatcg ttcgagttgg agtaagctca gatactattt cgcactttat atcgatacaa
    43561 cacaattaag aaatttagca tataccaaat tactatttgt tgattgtgtt aaaggcttgc
    43621 aaaaacatct taatgaactt gagcaggtgt aatcagatgg acctaaagac atttacagca
    43681 cagattgaac taatgcatca agaagcttta agacaaagtg tatcgtacga agacaagtgg
    43741 ctcaacacgt tccatggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa
    43801 ggagaatgtc gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc
    43861 agaagattgg caaaatgaca aagaagctgc ttcagaagtg atgaggcttg gaagagcgat
    43921 gtgggcagac aagagcaaca agccaagacc acgaaaaatc gcaatttggc acggtgacaa
    43981 acttctagtg atagggacag ctgaacagtt agcaagtctc acaggcttgc acgagaaaat
    44041 cgtgaggaaa agagcaaggt gtggctacac agacgctaag aagagaacgt ttagatacgt
    44101 ggaggaatca tcatgacaac agaagaagtg attcaaatgc gtattcgaag ccttcagcgt
    44161 gagattgacg atctggaacg aacaaaggca gtgatggtca atgaaacggc gagaaaggca
    44221 atcgatttgc acatagagaa tttaagaagg gaaatccatc gattggagga atgagcgtgg
    44281 ataagaaagc agcaatgaaa agaattgctg aattaaccaa gtcagaatct tggcaagaag
    44341 acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca
    44401 aacggagaac gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta
    44461 ctgctgaaca gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta
    44521 ggagcttatg gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga
    44581 tctcattaca caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga
    44641 taaaaaatca aaagaacgag ctgaattagt taagtt
//
LOCUS       DAZUMO010000019        43381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000019 DAZUMO010000000
VERSION     DAZUMO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.019"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..3308
                     /locus_tag="ACG8ER_001010"
     CDS             30..3308
                     /locus_tag="ACG8ER_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS5049609.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3849..5756)
                     /locus_tag="ACG8ER_001011"
     CDS             complement(3849..5756)
                     /locus_tag="ACG8ER_001011"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS5049610.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5886..6761
                     /locus_tag="ACG8ER_001012"
     CDS             5886..6761
                     /locus_tag="ACG8ER_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS5049611.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6841..7815
                     /locus_tag="ACG8ER_001013"
     CDS             6841..7815
                     /locus_tag="ACG8ER_001013"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5049612.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7995..9140
                     /locus_tag="ACG8ER_001014"
     CDS             7995..9140
                     /locus_tag="ACG8ER_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5049613.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9277..9621
                     /locus_tag="ACG8ER_001015"
     CDS             9277..9621
                     /locus_tag="ACG8ER_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS5049614.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9892..11178
                     /gene="efmA"
                     /locus_tag="ACG8ER_001016"
     CDS             9892..11178
                     /gene="efmA"
                     /locus_tag="ACG8ER_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS5049615.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11316..11915)
                     /locus_tag="ACG8ER_001017"
     CDS             complement(11316..11915)
                     /locus_tag="ACG8ER_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5049616.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12319..13440
                     /gene="mnmA"
                     /locus_tag="ACG8ER_001018"
     CDS             12319..13440
                     /gene="mnmA"
                     /locus_tag="ACG8ER_001018"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS5049617.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13686..15107
                     /gene="arcD"
                     /locus_tag="ACG8ER_001019"
     CDS             13686..15107
                     /gene="arcD"
                     /locus_tag="ACG8ER_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS5049618.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15121..16239
                     /locus_tag="ACG8ER_001020"
     CDS             15121..16239
                     /locus_tag="ACG8ER_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS5049619.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16299..17591)
                     /locus_tag="ACG8ER_001021"
     CDS             complement(16299..17591)
                     /locus_tag="ACG8ER_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5049620.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17741..18301
                     /locus_tag="ACG8ER_001022"
     CDS             17741..18301
                     /locus_tag="ACG8ER_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5049621.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18430..18807
                     /locus_tag="ACG8ER_001023"
     CDS             18430..18807
                     /locus_tag="ACG8ER_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS5049622.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18740..20812
                     /locus_tag="ACG8ER_001024"
     CDS             18740..20812
                     /locus_tag="ACG8ER_001024"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5049623.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21023..21970)
                     /locus_tag="ACG8ER_001025"
     CDS             complement(21023..21970)
                     /locus_tag="ACG8ER_001025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5049624.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22216..22680
                     /locus_tag="ACG8ER_001026"
     CDS             22216..22680
                     /locus_tag="ACG8ER_001026"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS5049625.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22742..23785)
                     /locus_tag="ACG8ER_001027"
     CDS             complement(22742..23785)
                     /locus_tag="ACG8ER_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS5049626.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24162..25127)
                     /locus_tag="ACG8ER_001028"
     CDS             complement(24162..25127)
                     /locus_tag="ACG8ER_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS5049627.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25361..26206
                     /locus_tag="ACG8ER_001029"
     CDS             25361..26206
                     /locus_tag="ACG8ER_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5049628.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26397..27584
                     /locus_tag="ACG8ER_001030"
     CDS             26397..27584
                     /locus_tag="ACG8ER_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5049629.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27590..27946
                     /locus_tag="ACG8ER_001031"
     CDS             27590..27946
                     /locus_tag="ACG8ER_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS5049630.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27948..28286
                     /locus_tag="ACG8ER_001032"
     CDS             27948..28286
                     /locus_tag="ACG8ER_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS5049631.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28362..28618
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28681..29715
                     /locus_tag="ACG8ER_001033"
     CDS             28681..29715
                     /locus_tag="ACG8ER_001033"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5049632.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29708..30394
                     /locus_tag="ACG8ER_001034"
     CDS             29708..30394
                     /locus_tag="ACG8ER_001034"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5049633.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30418..31266
                     /locus_tag="ACG8ER_001035"
     CDS             30418..31266
                     /locus_tag="ACG8ER_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5049634.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31463..32236
                     /gene="sufC"
                     /locus_tag="ACG8ER_001036"
     CDS             31463..32236
                     /gene="sufC"
                     /locus_tag="ACG8ER_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS5049635.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32249..33535
                     /gene="sufD"
                     /locus_tag="ACG8ER_001037"
     CDS             32249..33535
                     /gene="sufD"
                     /locus_tag="ACG8ER_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS5049636.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33532..34767
                     /locus_tag="ACG8ER_001038"
     CDS             33532..34767
                     /locus_tag="ACG8ER_001038"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS5049637.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34754..35224
                     /gene="sufU"
                     /locus_tag="ACG8ER_001039"
     CDS             34754..35224
                     /gene="sufU"
                     /locus_tag="ACG8ER_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS5049638.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35229..36620
                     /gene="sufB"
                     /locus_tag="ACG8ER_001040"
     CDS             35229..36620
                     /gene="sufB"
                     /locus_tag="ACG8ER_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS5049639.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36718..37860)
                     /locus_tag="ACG8ER_001041"
     CDS             complement(36718..37860)
                     /locus_tag="ACG8ER_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5049640.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(37911..38708)
                     /locus_tag="ACG8ER_001042"
     CDS             complement(37911..38708)
                     /locus_tag="ACG8ER_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5049641.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            38910..39209
                     /locus_tag="ACG8ER_001043"
     CDS             38910..39209
                     /locus_tag="ACG8ER_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049642.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            39474..40349
                     /locus_tag="ACG8ER_001044"
     CDS             39474..40349
                     /locus_tag="ACG8ER_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049643.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            40346..40564
                     /locus_tag="ACG8ER_001045"
     CDS             40346..40564
                     /locus_tag="ACG8ER_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049644.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            40551..43178
                     /locus_tag="ACG8ER_001046"
     CDS             40551..43178
                     /locus_tag="ACG8ER_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS5049645.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            43193..>43381
                     /locus_tag="ACG8ER_001047"
     CDS             43193..>43381
                     /locus_tag="ACG8ER_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049646.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSE"
BASE COUNT        14357 a         7675 c         9117 g        12232 t
ORIGIN      
        1 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
       61 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      121 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      181 caatggagga aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      241 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      301 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      361 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag
      421 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      481 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      541 gagacgcagt accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      601 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      661 gcggcagcaa atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat
      721 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      781 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
      841 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
      901 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
      961 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1021 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc
     1081 ctgcgacaga tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg
     1141 tgacctctag taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg
     1201 acggattatt gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact
     1261 tggctatcac tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1321 aatttacttt cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga
     1381 ctagttatga aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga
     1441 cagtttatgt aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat
     1501 ctctgacaca agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa
     1561 cttattcctt cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc
     1621 cgattcagtt gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag
     1681 gaactggaaa tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta
     1741 caaatagtgc agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag
     1801 agattcatgc ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc
     1861 ctttcgaagc atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     1921 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg
     1981 tatttgacgg ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa
     2041 atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct
     2101 gggtaccagg aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt
     2161 taacgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg
     2221 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2281 ataatgtggc tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct
     2341 tatctttccg aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2401 aagatcaaat ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2461 atactcttgg acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag
     2521 aaggtcagca gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc
     2581 agattaaacc ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg
     2641 taggtgctat ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg
     2701 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2761 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     2821 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     2881 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     2941 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3001 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3061 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3121 atgaatcacc aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag
     3181 aaagtgaaaa aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc
     3241 tagcattagc tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa
     3301 aaaaataagt ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat
     3361 agaaggaaat gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct
     3421 ccttgctgct tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3481 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata
     3541 tgaggaacta tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac
     3601 ctctcgataa acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa
     3661 tgtggaaagc cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca
     3721 acctctaata catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa
     3781 aaatttgaga agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc
     3841 acaacctctt accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc
     3901 gtgataccaa atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt
     3961 ttagcagggg catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt
     4021 gtacgccaaa tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt
     4081 tttgctgctt ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt
     4141 aattttccgt ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct
     4201 tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca
     4261 tattcgtcaa ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca
     4321 gctccgattc caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga
     4381 aggatcgctg ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg
     4441 tcagcactca tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct
     4501 tttaatgtga gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca
     4561 ggtactgttt tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata
     4621 cctaagttat ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt
     4681 tcttctagac gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt
     4741 ttagcagctt cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt
     4801 ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat
     4861 acgcgagaat agattccgct gacttggcgg aattcttctg atagataacc actaagagaa
     4921 cgtactgttt taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg
     4981 tcattcaaat gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca
     5041 atcaaacccg tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca
     5101 aaagattgcg gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga
     5161 ggagcaatca atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt
     5221 tctttgccgg caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt
     5281 gcaccgttgg gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga
     5341 taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat
     5401 ggcaatggta atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac
     5461 tgcaatgatt caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt
     5521 cggtcataat cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac
     5581 actttttctg gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg
     5641 tcaactaaat cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt
     5701 tgccattctt cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat
     5761 cgatcgctcc ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat
     5821 agaacataaa aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac
     5881 ttataatgaa aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt
     5941 tcaaagtaga taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa
     6001 gtaaattgct ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat
     6061 caggacatcc ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta
     6121 aaaaatggaa gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag
     6181 aagatattcc cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg
     6241 tgggattgcc agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc
     6301 tttgtggtac ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt
     6361 ggatgcttcc aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg
     6421 cattgcgtag ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag
     6481 ggatttctag ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag
     6541 gttcatggca gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt
     6601 tgaaagtact tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa
     6661 ctgaaacagg agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca
     6721 ttcctgccag ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca
     6781 gaaaatcttg gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac
     6841 atgagtaata agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca
     6901 ttagctgaaa aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa
     6961 tttagtgatg gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat
     7021 atcgttcaat caacgaatgc acctgtcaat gattactata tggaactact gatcatgatc
     7081 gatgcaatga agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat
     7141 gctaggcaag accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac
     7201 ttgatcgaag aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta
     7261 caaggattct ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat
     7321 taccgtcaat taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt
     7381 gttcagcgtg cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat
     7441 gcagatgatg cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt
     7501 atcatggtag atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta
     7561 aaagaaaacg gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat
     7621 gcgaaaagta ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc
     7681 acagaagaaa atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatgggt
     7741 gaagcggtca aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa
     7801 aaagaattcg aataaacaaa aaaaagaggg aaagaagttg tgacagaagt gagcagcttc
     7861 aaggagctgc gacaagactt ttgtcacagc tccttttttc tatgcccatg aaacgtcctc
     7921 tagacaaaaa tgagctcttc tttggtttgt tcgctatttt atgctagaat gtgcctagca
     7981 ataatttgga agggatgaat gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc
     8041 ccatgcatcc acaagtaatt gaagcgatga cggcaatcat gcaagacacg tttggaaatc
     8101 cttcaagcat acatggattt ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa
     8161 tcattgcaga cagtctgcaa gcaaaacctc acgagatcat ctttaacagt gggggaacag
     8221 aaggggataa tacagctatc ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc
     8281 taatcactac agctatcgaa catcctgctg ttcttaatac catgaaatat ttagagacaa
     8341 aggggtttga cgttacatat ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg
     8401 tcaaaaaagc gttacgagaa gatacgatct tggtctctgt gatgtacggg aacaatgaaa
     8461 tcggaaattt gatgccaatc aaagaaatcg gtgaactttt agcggatcat ccagcttatt
     8521 tccatacaga tgcggtacaa gcgtacggaa accaaacgat ccatccagaa gaattaggaa
     8581 tcgatttatt aagtatctct gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat
     8641 ataaacgaga cggagtaaat atccctgcat tgcttttagg cggggagcag gaagaaaagc
     8701 ggcgtgcagg tacagaaaat ttagcaggga tctggggtat ggcaaaagca gttgaactgc
     8761 tgtcacctga agaacgtgaa gcacgtaaca acaaatataa aggattccag caattgattc
     8821 ttgatacatt agaagcaaac gaaatccctt atcaagtaaa tggtgattta acgaataaac
     8881 tccctcatgt gttgaatctt cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc
     8941 ttgatcttaa tggggtcgct atttctactg gttcagcttg tacagcagga aatatcgagc
     9001 cttcacacgt attagaagca atgtatggag aagatacacc gatactaaaa gaatccatcc
     9061 gtatcagctt tggtttggga aataccgaag acgacatcca gtatttcctt gatcaattag
     9121 ttcgtgctgt caaaaaataa ctagtgaagt cattcttata tgaacagatg tctgagagaa
     9181 cgccttcatt ttttagggaa cttaactgaa aaacctagta aaaatcagga taaactttta
     9241 taataaagag aaactaattt tatcgaggtg aaaatcatgg catttgaaac aacagcaacg
     9301 gtactgggcg caccaattag ctatcgtttg catcctgatg caaaaagata tacattgcgc
     9361 gataatggat ttacagaaac aaacggaggc aattatcaat tgatccgacc attagatgcc
     9421 acgccgcaaa gcaaagaagg attcaaatta aaaatcactg tatcaaaaga tattcaaaca
     9481 ttgaaaatgt cgatcactac agcaaatgga ttgaaagcag tcaatatttt caaagatcca
     9541 aaacagaaaa tgagccaaga taaattttat tttctgatgg atgggatgat tagccgtggg
     9601 ttattcgaaa aagtcgaatg atctgtggat ataaaaagac aactagtttt gaaacggcct
     9661 attgttagat gactaacttt gtaaggacga cgcaaattag ttgtcttttt tatttttaaa
     9721 tttaacacat gttgttattt ttaatatttt gctgatttat tgtttctaag taaagaaatt
     9781 taaattgaaa cgattgttat ttagggaaaa aggtattttt tgaaaaaaga atcccgtata
     9841 atgattgttg tgcaaacgct atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat
     9901 gaacagtcgg tcgttttaac gaattggaag agaaattatt tgttcttttt atcgggacag
     9961 tttttgtcag gaatcactag tatggtcgtc caatatgcga tcatctggta tttgacaaga
    10021 gaaaccggtt cggcgacaat tttgagtttt gctactttac taggaatgat tcccatggtt
    10081 ttactaagcc catttgtagg accgctagtg gatcgctggg ataaaaaagc tcttttgatt
    10141 gtcacggata ttattgtagc gattttcgcg cttattttag cagtagtcgg aacgatttca
    10201 gaatccttcc cgatttggct agtttttgtg tctttgttta tgcgttcggt cgcacaaaca
    10261 ttccaaatgc caacgatcca gtcgattatg ccgacaatcg ttccatcttc tcacataaca
    10321 aggacgaatg gacagttagg aatggtccaa tcagcgaatt tcatcattgc gccagctcta
    10381 ggtgcggctt tgttttcagt cgttcctgtc aattacctta ttttattaga tgtattgggg
    10441 gcagtttttg gtgtcggctt attgattttt gtgaagatcc caaaagtttc tcctgaaata
    10501 ttggaagttc cgcttactat ttttaaagat gcaaaatttg ggctgcagca attgatggat
    10561 aataaagggt tatggtacat aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca
    10621 gcaatcagtc tgtatccatt gatgacgcta gattactttg gtggttccgt tggacaggcg
    10681 ggagcagtgg aagtagtcta cgcagtcgga atgctcttag gcggtgcact gatcagtttt
    10741 ataggaacat ggaaagaccg aatgaaacct attattatag cctacatcat tatgggcctg
    10801 acgatcggtg caagcggatt ggttccaaac gatagtcaag ggttcttgta ctttcttatc
    10861 ttgaatgcag gagcaggttg tgcaacgcca tatttcaata cactgctgat ggcaatgatc
    10921 cagcagagtt atgaatcgaa tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg
    10981 aacctggcag gtccaattgg attattattt gcaggaccat tagctgatcg tttaggggta
    11041 gaaaaaatgt tcttgttttc cggaatcggg attttattat gcggaatcgt tttattttta
    11101 acgtcagctg cccgaaatta tgataaagaa ttacagaaaa aactggtaaa agaacaccat
    11161 gagcaaaaag acgaatagta tggaatggag ttgtgacaga agtcttgtta caactccatt
    11221 tctgatccta atgttcctgt ggcaataagc ctactcaaat gaaaaatatg agtaattatc
    11281 tttatttgtt taatacttga tatagggcag ttttttcacg acctatcgta cttcctcttt
    11341 gcttcataga tttctttgat ttgttgctca gataacggag tgccaaatgg tagtttttcc
    11401 tttaagagtt ctctttccgg tgcgtctacg cctacattga catcgttctc gataggtacg
    11461 ctgtaatgat ggatatgtcc atgaagattg cggatagtgt cgttaggccc gagcaacatc
    11521 ggataatgag tcaagtaata ttgttgatgg tcgaatttaa cgatggcacc tacatcataa
    11581 aaataaaatt tgggacttcc gtcagtaagc ctaggatcat ttttttctaa ataacggaaa
    11641 aaagcgcgac tgtcatggtt ccctttgatc aagtggattg ttccttgcag ttgagtgacc
    11701 agttccaaaa tttctgcatt tgctttttca tattgcggat gcatagcaag gtcgcctaga
    11761 tgataaacat ggtcggtttc ttttattact gcattccagc tagtgatcaa ctgttggttc
    11821 atttcatcca catttgcaaa gagccgcgga gcaaagtcgt tttttcctaa taagcgttca
    11881 tggaaaaagt gcatatcact gataaagtaa ttcatgtatc ttttgcctcc cttttttctt
    11941 agagtaaaca aaatcactta tctgagcaac tcataacatc taaaaagaaa aggttgtaaa
    12001 tgagacgtgc agctttaagc gataagaagc aattttgaaa aatagttttt cacaatttat
    12061 aaaattattc cttattgccg aaaggctggc cctttttatg agtatgccaa ttttttatat
    12121 cgaatgctca aaagatgagt gattttttgg ttgactatca tgtttgtgta ctgaataggt
    12181 ccagtagaac agtattttta cgacttgata attaagggat gaaattttca gaaatatgac
    12241 gtataatgta aaatactgaa aagttgcgac cttcaactcg tagatgtttc agaatctaat
    12301 tgaaatgatg gtgatatcat gacagacaac agcaaaacac gcgttgtcgt agggatgagc
    12361 ggcggtgtcg actcatctgt aacagcactt ttgttaaagg aacaaggata cgacgtgatc
    12421 ggaatattca tgaaaaattg ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa
    12481 gattataagg atgtagcgaa agtagctgct caaatcggga ttccttatta ctctgtgaat
    12541 tttgaaaaag aatattggga tcgtgtattt gaatatttcc tagccgaata ccgcgcagga
    12601 cgtacaccta acccagacgt gatgtgcaac aaagaaatca aattcaaagc attcttggat
    12661 tatgcaatgg atcttggcgc cgattatgta gctactggac attatgcgca agtcactcgc
    12721 gatgaaaacg gcgtggtcca catgcttcgt ggagtagaca ataataaaga tcaaacttac
    12781 ttccttagcc aattatcaca agaacagctt tcaaaaacga tgttccctct aggcggcatg
    12841 gaaaaatcag aagtacgggc aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa
    12901 gattcaacgg gaatctgctt tattggtgaa aagaacttca aacaattttt gagcaactat
    12961 ctgccagcaa aaaaaggaaa tatggtcact cttgatggtg aagtcaaagg acaacatgct
    13021 ggcttgatgt attatacgat tggtcaacgt caaggactag ggatcggtgg cggaggagat
    13081 tcgcaagaac cctggtttgt tgtaggaaaa gatctagcga caaacacatt atatgtgggg
    13141 caagggttcc atcatcctgc attgtatgcc acaagcttgg atgcaagtga aatccacttt
    13201 acgacgaatg aaccaatgcc aaaagaattc aagtgtacag cgaaattccg ttatcgtcag
    13261 caagacgttc ctgtgacagt tcgcttattg gatgataatc gtgcagaagt cgtattcgac
    13321 gaacctgtac gtgcaatcac tcctggacaa gcagttgtat tttatgatgg aatggaatgt
    13381 ctgggtggtg gtttgatcga ccacgcatat caagaaacaa aagttttaca atacgtttaa
    13441 gcgatattaa atgaatcaaa acagctatgg ctaacaaaag aaacaccttt tcttttggat
    13501 agcatagctg tttttttgca ataaaattaa tttttattca ttttatactt gaaatacttg
    13561 tgtaaaaagt tcataatatc caataatatg aataaagttg ctttttgtta agcaagagca
    13621 gttgcaaaat agaagggaat cttttgcaag tgatttgttc aattttataa gtgagggtga
    13681 tggaaatgga aaagaaatcg acgggtatta gcaaatgggg actggttgct ctggttgtca
    13741 gttcgtcaat cggaagcggt gtgtttggga tcacaagtga tctggctagt tctgcagctc
    13801 cagggccagc gatcctttca tggataatcg ttgggattgg tattttagct ttggtattat
    13861 cgctgaatca tttaggagaa aagcgtccag atctagatgg tggtattttc ggttacgcgg
    13921 aagcttcatt tggtaaatta ggtggcttca tcagtggttg gggttactgg ttgtctgcgt
    13981 ggctgggaaa tgttgctttt gcaacgatgc tgatgagcgc catgggagaa tttttcccga
    14041 tattcaaagg tggacaaaat gttccttcta ttttatttgc aagtattttt atttggggtc
    14101 tgactttatt agtaaacaat ggagttgaag gagcgagcgt catcaataca atcgtgacga
    14161 tctgtaaatt agtgccatta ttcctatttt taatatttgt atttatcgca tttaaagtga
    14221 atatattcac tgctgatttt tggggtaacg tatctgataa tctagtgaat ggaacagggc
    14281 agcaaggaac gatctggttg cagattaaag gttgtttgat ggttatgatg tgggtctttg
    14341 tagggatcga aggcgcaagt gttttagcca accgcgcgaa gaaacgttca gaagcccagc
    14401 aagcatcgat cattggtctg ctttgtttat tgtttatcta tatccttgct tcgatcttgc
    14461 catacggcgt cttatctcaa gaagagttag caactatttc acaacctgct atggcaaata
    14521 ttttaaaagg aatcgtggga aattggggag cagcgctgat caatatcggc ttgattattt
    14581 cgatcattgg ttcttggttg tcttggacga tgctcccagc agaaacaacg atgttgatgg
    14641 ctcgagacgg tctgttgccg aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt
    14701 attcgttagt tgttactgca atcttgacta atctattctt attgactttc ttagttactg
    14761 attatgccta tcaatttgcc tattctttat gtacagcggc aattttgatt tgttatctac
    14821 ttgtcgggat ctatcaagtc cagttttctt taaaagaagg aatcaaaaaa caagtagtga
    14881 tcggtgggat tgctgtagtt tttgaattga tgggcatcat tttagcagga ttttcttatg
    14941 tccttttatg tagtattgcc tatcttcctg gtttttgctt ctactggatc gcttgtcgtg
    15001 aaaccaaaca tcaaattacg ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg
    15061 cactgctttc aatcgggttg cttgctgtag gctggatcaa gatataaggg ggaaagaaac
    15121 atgaaaatcg caaatttcgg tgtagaagaa tggctaaata aatgggaaaa gaaagcggta
    15181 tatgatattg cacaaagctc gatcgaagca ttgacattag acgaactcgt tggcttagat
    15241 gggactagcg taagtaaatt tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg
    15301 atcgaaggat cagatgtttt caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg
    15361 gaaaacatct tacaaacgaa tggagcgact ggtgctaatc ttttagctct atacgcgtta
    15421 gtggaagcag gagaccatgt tgtttcgatg cttcctactt atcagcaatt atatgacatc
    15481 cccaaatctt taggtgccgc tgttgacttt gttcatttga aggaagaaga agattggcag
    15541 tttgatttag aacagctaga agccttagtc aaacctgaaa caaaaatcat ctgtttgaat
    15601 agtgcaaaca atcctacagg cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa
    15661 attgctcgta cagtagatgc atatgtattg atagatgaag tatatgcgcc tttaacggat
    15721 aaaggtgaat tcttgtcgat cgtagatgtc tatgaaaaag gtatagcaac caattcccta
    15781 tccaaaacct actccattcc aggtattcgt attggctgga cagctactgg acctgagttg
    15841 gcagaagttt ttcgaaagta tcgagactac acgatgattt gcggcggagt cctgtcagat
    15901 gatcttgctg tacatgcatt gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc
    15961 attacagaga accttgtgat tttaaaacaa tgggtagcca atgaaccgaa agttgaactt
    16021 gtcgctccca attacgtctc tacttctttc attaaattag ccatcgaaga agatgaccag
    16081 acattttgta tcaatttatt agaagaaaca ggagtactat tagtaccagg aagtgccttt
    16141 gatttaccga agcatgcccg actaggttac tgttgtaaaa aagaaacctt ggaaaaggga
    16201 ttattcctct tatctgagtt cttgaaaaaa caggcctaaa caaaaacact gcaaggacag
    16261 tctgtgacaa ctgttcaagc agtgtttttc atttacgctt attgtctatg agaaggagag
    16321 tgccctgaag ctatggagac tcctgaagct ttttcttctg cagtttcttg ctgatggatc
    16381 tcttgtgtat tgtgtcgtac aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga
    16441 aagacgccgc gataaccaaa tgcactggaa acactggagc cgatcatagg ccctactacg
    16501 ttaccagttg cttggaaaga ttggttataa ctgaagatcc gtccagcagc ttgatgaggc
    16561 gaatattttg tgatcaatgc ttggacagca gggagcagac aagcatcaga gataccaatc
    16621 aaaaaacgta aggcagctaa ttgccaaaca ttcgtgacga atgccattgg gatataaacc
    16681 agaatcgcaa aacccaaacc aatcgcaagt attctttcac tcccaatctt gtcacctaag
    16741 cgaccaaatc ttggagcggc tatcaaagtg gcaatcccag gtatcgaagc aatgattccg
    16801 ctgatcaacg taacattccc atgtccatgc agcaattggc gaatgtacag actgatgatt
    16861 ggactaatgg aattgttgga tgcttggatg atcatcgttg tgacgaacat accgatgacg
    16921 acgtgtggat atttcaagtc tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc
    16981 actgggacaa atttttcatg gacgaaaaac agactcaaca agaagacgaa aaataagatg
    17041 atccctgtaa tgaaaaaggt agggcgatag ccaaacgctg aagcagagat cccgccaaga
    17101 agcggtccaa gtaatgttcc tgtaacagaa ccagttgcta aggttccaag aacttgacca
    17161 cttttttctt ttggcgtccc agttgcgaca agggcagtgg cattactgat ataaccagaa
    17221 aaaatccctt gaagcagacg caatgcgact aattgataca cactagtcac gcaacccatc
    17281 aaagaaatga cgattgccat tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt
    17341 tggtcggcta attttcccca ccaaggagag ataagcgtag tcaccaagaa agtagatgaa
    17401 aaagtcaatc cgctccaaaa gttcaattgt gacgtactat aattacctaa cgtatctatg
    17461 taaagtgaca taaaaggcat gactaaacta aatccgatac cagccataaa tgttccaaac
    17521 cataagacaa acaagttttg ttcccaagtt tctcttttcc taaataattt ttcctttatt
    17581 cttgcagcca attagactcc ttctttctga aaattttcat ctacagctct ttattataaa
    17641 ctacaatatg tagtttatca atgaaagtct cttttttctc tttctttttc gtggtaaact
    17701 gaaagagtga aggatttatt ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc
    17761 tcaagagaaa atcatcgact gctttcgaga actagcagaa gagatggatg ttcagcaaat
    17821 aacctttcag catttagcca aacgcttgga tatcaaggct ccctcgctgt ataaccattt
    17881 caaaaatatt cgtgatgtca aaacagcgct aacagcgaaa cttctgcagg aacttaatga
    17941 ccagctgcga cgggctcttg tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga
    18001 aacttatcga tcctttgctt tttcaaatca agcagtgtat gagttactga tcagtgtacc
    18061 gcataccaat gaagctgttt tattagatgg aatccatgaa acgaatcaga tcattttgca
    18121 gatatttgag gcttttcaat taacgaaaga agaaaaagtt catcgaagca gggaacttcg
    18181 tagtatgatc cacggttatc tttccttacg ttttttagga tactttacaa aagaaccaac
    18241 aatcgatcca gaagacagct atttttggat gatcaataac tttatcgcta ctttgcctta
    18301 aacaatcaga ttcatttgta acaattttaa gatgttcatt aaatgaccct tttataagag
    18361 aaaattcttg caactttctt gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg
    18421 agcacgagta tgtatcaagt aattaccatg ttcggggaca atgagccttg gtggtttttt
    18481 gaagactggg aagaagatat tgaagaagaa gaaacattcg agtctttcga agaagcacga
    18541 caagcgtacg aaaaacaatg gaacgaaatc catcaaaatt atgaatatat caatgcaaaa
    18601 agtaactttc tcagcgcctt ttggaatgac ggagatgagc gctggtgtga agagtgtgac
    18661 gatgatttac agcaatacaa gggacttgcg ttgttgaaaa atcatcagcc catcacagta
    18721 gaaagcagaa aggaatttta tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa
    18781 agacctaagc aaggtgcttg gtgctaatca aaagcacaaa aattattatg aaggacccaa
    18841 ggtgatcgtt acttgggccc ttggtcattt attaggattg aaaatgccgg aagatctgaa
    18901 taaggaatgg cagagctggc aaatggacac gttgccaatg atccctaaaa aactaggaat
    18961 caaaccactt cctaaaacag gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa
    19021 agatgtctca gaagcagtca ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg
    19081 ctggatctta gagtatgtgc atttcaataa accagtgaag cggttatgga tctcttctca
    19141 aacagataaa gcaatcaaaa ctggattcaa gcaactaaga cctgcgaaag aatatgatgc
    19201 attgtatgaa tctgctcttg cccgggcaaa agcggactgg ctggtaggat tgaatgtgac
    19261 cagagcctta actgtgaagt acgcggataa tttatcagca ggccgtgtgc agacaccgac
    19321 actagcatta gtgagagacc aagaaaagaa aatcgagcag ttccgtccac aaacgtattt
    19381 cacgatcacg ttgcaagctg gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc
    19441 tttaaaaaca caagaagaag cacaaaaact tgttcaattg atcagtacaa gcaaaggaac
    19501 tgtcacagaa gtccaagaaa aattaaaaac ggaatctgcg ccattgcctt atgatttaac
    19561 agagatccaa agagaagcca atcaacgcta tggctattct gcaaagaaaa cattaagtct
    19621 tgtacaaagt ttgtatgaga cccataagat cgttacttat ccgcgtacag acagcaaata
    19681 tctgacaaat gacatgaaaa gtacgatgaa agaacgattg caagcagtca gcgactttgc
    19741 tcctgaggtc aagcagtatt tgaaaaacgg agccatcgtc caacagacaa aagtattcca
    19801 agacagtaaa gtgaccgatc accatgcact tttgccaact gaaaacagag cacgttatga
    19861 aaaattaagt aatgaagagc gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact
    19921 gtttgctcaa ccccataaag tcagccaaac aaaagttacg gttgaatttg gtaaagaacg
    19981 attcattttt cggcaaaatc gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga
    20041 gacggaaaca gtcaaatggg aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa
    20101 agaactatct gcaccgccta aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa
    20161 atacagtctg ggaaccccag ctacgcgtgc agagattatc gagaaactaa tcaaatctga
    20221 actgatggaa cgtacccctc aaggactgca agtatctcct aaaggtaagc aattattagt
    20281 attggtaaat ccctctttag taacaccaga tttgacagaa agctgggaaa aagaccttga
    20341 agctattgct gcaggaaaga aacaagcagg aaccttcttg aatggaatcg aaaaagaaac
    20401 aaaacgcttg gtgaatgaga tcaaatcaag caaacaagaa tatcaagatt tctcgattac
    20461 ccaaaagaaa tgcccagaat gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa
    20521 aatctatgtc tgcaccaatt cagattgcaa ctaccgcaga agaaaagatc caaaagtctc
    20581 taatcatcga tgcccgcaat gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc
    20641 ttatttccgt tgtaaatacg atggaacgac agaaaaattg cttgataaaa aagagcagaa
    20701 gaagaaaatg accaaacatg aagaacgacg cttgctgaaa aaatatgcac aagcagaaga
    20761 accagaagaa agcccattag cagcagcact aaaagcagca atgaagaagt aataagatat
    20821 gaaagaggtt gtgatagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg
    20881 cttctcaaat ttttgtgaaa tttcggctta tttccgaaga agctacttct ggaacaccat
    20941 ttattcggaa aaaaggactg tgacaagact tttgtcacag tcctttttca tttaaaaaaa
    21001 tgtttacttc atttgacgta tattattggt gaggcttcaa ttcatttggc ttcacttttt
    21061 ctgtttctct tatgatataa acaggacgct gtttcgtttc taagaagatt ttcccaatat
    21121 atttcccgat gattccaaga cataacaatt ggattccacc aatgaacagg aagatcgaga
    21181 ccatggaagg ccatcctgat gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca
    21241 aaattgcaat accagaaccg acacaagaca aagcgccgat aaatgaagca atcgttaacg
    21301 gtgcatccga gaagttcacg ataccatcaa tcgagtagtt gaataatttc cagaaggacc
    21361 aagatgtttc gccagccaca cgttcgcggt tttcataaga aatatattct gttttaaatc
    21421 cgacccagct aaataatcct tttgaaaatc ggttgtattc tgttaattct aggacggcat
    21481 ccaccatttg acgagtcatc aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc
    21541 tgattttatt gatcaactgg tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc
    21601 cttcacgagt cgaacgccgg gtgccgacac aatcgatatc agaagtagtg atccggttga
    21661 tcatctcagg aagcagttcg ggcggatctt gcagatcaac atccatcacg gtcactaaat
    21721 ctcctgtaga agcttgcaat ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa
    21781 atgagacaaa gcggacataa ggataagctg aggctagctt cttgattgct gctaacgtac
    21841 cgtcttttga tccgtcgtca ataaacaaat attcgaactc atgggcacct ttgaattttt
    21901 caacctcatc aaaaaaaaga ggaacagttt tttcttcatt aaaacatggg acaacaattg
    21961 atactttcat cgtttctatc ctttcaatcg tatgtttata tatggtttac attcaaaatt
    22021 taataaacct taaataacac ctttaaaatt tgattttacg taaaagaaca ggtattcttt
    22081 ttgtaaacta acgtatttag tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa
    22141 tttttattag ttggcaagta gttttggata gagaaaaagg aggaagttcc agtctattga
    22201 ctaggagcaa acaaaatgaa aatacacaca cctatcggct ttttatggtt agatgcagat
    22261 gaatccggat tgactgctgt atcttttcag ccaatttcag aaagcgaatc agcgaatcag
    22321 cgaattttaa cgcgcacaca agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa
    22381 tttgatgttc ctttatctat aaagagaggt acgccttttc agcaaaatgt ctggaaagct
    22441 ttgtcgacca ttccttatgg agaaatccga acgtataaag aagtagccgt tgctgttgat
    22501 agtccgaaag ctgtgcgagc gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt
    22561 attccttgtc atcgagtgat cgggcaaaat ggaaaactga caggatatat gggaaacgca
    22621 atagatgggg ttgaaatcaa acgccaattg ttgttgttag aaggatatct gcaaaattaa
    22681 aaacagttgt gattcggaca ttgttccatg cgagaatcac aactgttttt tcatgttcat
    22741 tctaaacttt gatcagccaa ataaatagta aagccatcac tgtttggaga agcccgacag
    22801 ctaatccata agataagaag cgtgtacctt ctttcatgaa ttttgcaaag tctaaacgca
    22861 gaccaattgc cgctaaagca gtgatctcaa accagctgct gataaaatgt gccgattggt
    22921 caataactac aggtaaagaa aacaagctgt tgaccaaaca aaagatcaca aaccctaata
    22981 cataccaagg aagcggtaat tttcgttttt tctgcgtatc agttggcgtt tcaaacagag
    23041 ctggttgttt cttgtgcatg aatttttcaa acagatagac aacaacaacg agcatcataa
    23101 tacgtagaat cttaaatagc atggcgaatt cgacaacaga attgtcaagt aagcttgccc
    23161 cagcgactac ttgaccaaca gattggagtg taccacccaa aagtgcactt ttttcaatga
    23221 gatttccacc aaacaactgt aatccaagaa aagggagagt caacatcatc acagtaccca
    23281 gtagattgac tagtgtgatg atctgtcctt tttcttcctc tttcgcatgg atggaagggg
    23341 cgattgaagc aatagcagaa gaaccacaaa cagcgtttcc tccagccatc atcaaagaag
    23401 aagtatcgct gaattgcagt tttttaccaa tcaagtaagc accgatgata gtgactgaca
    23461 tttgcagtaa cacaaaaaca attcctttta caccaatttg agcaatcgtt tgagctgtga
    23521 cagttgcacc tagtaaaaaa acagagaatt ctaatagttt gctttcagca aattttgtgc
    23581 cgattgctag aacaggctgt ttaaaaaaag tatttcctaa taaaataccc aataaaatag
    23641 caatagtcgc tccacctaac tgtggcagat aaatagcaag aagcctgctg aaggcagcga
    23701 tggcaaaaga aagcagtaaa ccgggtaaga tttcgcggac cgctgaatcc tttacagatt
    23761 ttattggatg tgacaatgat ttcatttgat cggttccttt cgtcaataaa tactaaaagc
    23821 atactcgaac atagtagcac taaaccagta ttttgacaaa agaacaagag gtcgtgacaa
    23881 aagtcttgta cgacctcttg tttcgaataa acggtgttcc agaagtagct tctacggaaa
    23941 taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    24001 aagctgacca cttcttttac gacctcttgg taaacggtgt tcaaaagctt gcatctgcgg
    24061 taataagccc aaccagtaca aaaacatgaa cagctgtttt tgtttggccg ttcttattac
    24121 ttgatgcaga acagcttttt cacaacctat tgaatcgatt attaagcgat ttttttagaa
    24181 acaaattttc catgtttgct atgttcttta attaagaaac gtacgtaatg agcaagaacg
    24241 tagaagacaa ctacacttgc aaacagacat tccgcaactt ttaagaaatc caatacgcta
    24301 ctatagatac ctaaactgtt gaagtaatga acagtagtaa atatagctgt tgcagagata
    24361 acaagcggga atgtaaaggc tgcatagctt ggataaaaac gaagcttgaa caaacgtgga
    24421 agcatagaca gcacaacgaa atacaatact tgagataaag cgaacaatgc tacgacaaga
    24481 taaaaattag gttcagcaaa gtttttaaga taacctgtta ggcaaagaga accaggtgct
    24541 gcaacgatcg taatcagcgg acgtgtcgga tgagccatat ttcttacgaa aaagacacgt
    24601 ttgatgatga tcggcaataa gaacgcatag cagatcaatg aaatccaaaa gaaaattttt
    24661 ccaataagtg ggaagaagtt cccggcagtt acagtgatga ccccaaatcc aacgtaaatg
    24721 ataaaccagc ttggataaat tgcttcaatt gaaagatcag ctttgataac atggtgataa
    24781 ttgaaataaa agacaaggat cacttggaag ataacagcgc acagccaaat gaatttgaca
    24841 attggtgcaa tactttcata tgaaacaaaa taagtacaaa taaccatgag tgacatcgta
    24901 aaagttggtg aaacggaggc gatgattgga tcatgcaacg tttgtttcgt gtgttcgaat
    24961 aagataaaca atttaacaaa aatgaatatc ataagaatca tagagatacc gccagttaga
    25021 ttgcctaaag caacatggtg atagtcttta aacagatttc caagggaagc taacccaagg
    25081 atcaacccgc agatcgggat cggaataatt ttcaaaaagt tcttcatata taatattctc
    25141 ctttttattt aatagatttt agtaccaaga tgaacgaata cattgtaaaa tgaataggaa
    25201 tgtatgatta cgagcaagca gacagctgat tgtcttcatc taagttacta gttggatcag
    25261 caactgtttt gttgtacatt tctattatag atggtttttt gtatatttaa atcaaatagt
    25321 ttttatagta attatcataa aaagttatgg aagtggtgcc atgtttaagt tattaacgac
    25381 ttttcgagca gtatatgaaa caagaaattt ttccagagca gcagaagtac tgtttttatc
    25441 tcaaccagca gtctcgaatc aaatcaaaca gttggagcgg gaattgaata tcgatctttt
    25501 tcaacgtaac ggaagaaaag agatgcttcc aactaagcaa gccgacttat tatatgaacg
    25561 gacactggtt ttgttagaag aatgggaaga aactcagcaa aaattgctca atgaacaaaa
    25621 tgaagaagaa gtatgtcgga tcgttgcttc ccacaccttt gctgtctatc ttttgcctca
    25681 gttgatgaaa catctgatca aaaggtttcc taaagtgcat tttgagatcg aaatcacgaa
    25741 ttctcatgaa tcattagaac aagtagcgaa acatgaatcc gattttggtt ttatcgaaaa
    25801 gccattagag acttccggcg tacaaagata ctctttgatg gaagaccagc tagtcatcgc
    25861 tggggaccca aaaagcaaat tgtggcttgt aagagaagca aactctggtg tttttcatta
    25921 cacgcagcgt tattttgaag agatgaatat ccaagataag aagatgatcg tgaaaagtaa
    25981 cgatgtaatt gttgcgttgc tgaaggaagg aatcggtcgc tcgattttgt ctaagcgctc
    26041 tgttccagaa aatgtgccaa tgactccgtt gaatttccat cgatattttt atttcatcca
    26101 acgaaccaat ctggtttctg atcagctgct agagattgcc gaagagatcc ttcgatacaa
    26161 tgaaagcaca gaaacttctc acagcgagga gaatctcagc tggtagataa ttgcatgtat
    26221 caaaaaaagt tctatgatac actaaagata agcagtaggc ttttgtactg catttgctca
    26281 agcaactgca caaacggaaa gttaattttt cccaaatgtc tatggaaaga ctttccatta
    26341 tctaactgga ccgctataat aatcagcagt atggttttta agaaaaggga tgaaacatgg
    26401 aaaaaaaatt aaaagcttcg aacgataatc gtattgatta tggcgtgatt ttaccagtct
    26461 ttctgttatg tttgattggt ctgctctcat tatatgtagc acttagccat gatccgaaca
    26521 atccagcagt cttgaaaggc gtagggatgc aagttgtctg gtatttagtt ggagcggcag
    26581 caatcgttgt catcatgcat ttgaattcca aatggatctg gaaactgact ccttaccttt
    26641 atggtttggg gttagtggta atgggactct tgctcaaatt ctatgatcag aatttagctg
    26701 cttcaacagg gtctaaaaac tggtttcgat tcggtgcgtt tactttccag ccagcagagt
    26761 tgatgaagat tgcgtacatc ttgatgatgg cactgatcgt aactaaacat aatacgcaag
    26821 taaaagaacg gacgatgaaa tcggactttt ggctgattgg gaagctcttg atcgtgacaa
    26881 taccggtttt agcattgatc atggcccaag atgactttgg aacgatgctt gtctttcttg
    26941 cgatctttgg cgggattttc ctgatgtctg gaatctcttg gcgcatcatc gcacctgtag
    27001 taatcttggc agtagtcgtt ggagcaggaa cgatcttctt ggttacgact gaaggcggaa
    27061 gagacttgct ttataaagtt ggattcaaat cttatcagtt tgcacgtatt gattcatggc
    27121 tggacccttt ccatgatacg tcaggaatga gttatcagcc ggcccaagga cttttggcaa
    27181 ttggtaccgg tggcttgttt ggtaaaggat tcaacgtaag caatatctat gtgcctgttc
    27241 gtgagtccga tatgattttt accgttatcg gtgaaaactt cggctttatc ggtggtgcat
    27301 tcgtcatttt cttgtatttt attttaatct atcgtatgat tcgggtgtgt ttcgatacga
    27361 ataatgaatt ttacgcctat atcgcaagcg gtttgatcat gatgctgctg ttccatgtct
    27421 ttgagaatat tggagcaaat atcggattac ttcctttaac agggatccca ttgcctttca
    27481 ttagtcaggg aggatcctcg attttaggga acatgatcgg tatcgggttg atattatcga
    27541 tgagatacca aaatgaagca cctgcaagat cttcaaggag gtaagaaaat tgtacacctt
    27601 ttactggtac ccgaaatgtt caacatgcaa aaaagccaaa gcgtggttag accagcatgg
    27661 cgttagttat caaaccgttg atatgatcaa agaagtaccg aaggcctcaa cgattgagac
    27721 ttggctagaa acgagtcaag taccaatccg tcgcctgttc aacacaagcg ggatgaaata
    27781 tcgggaactt ggactgaaag atcaagtagg cgagtttacg gcttcaaaag cgagccaagt
    27841 cttatcgaca gatggtatgc tgatcaagcg accactgatc ttacgagagg accagcttgt
    27901 tgcaattgga ttcaatgaaa aaatttatga aggagaactg atataaaatg gcaggcaagc
    27961 atttgaagaa aaaggacaac ctttggatct tatttaatgg aaaagaatac tgtgttggtt
    28021 tgacaaatga agcgcaagaa gaacttggag acatcacgtt tgccaatctg ccaaaagcag
    28081 gacaatcgct aaaaaaagat gaacctctga tcgaagttga agcggaaaaa gcggtcaatg
    28141 aatttgccag cccgctaacg ggtacagttt cttctgttaa tgataaaatc ggtgaagata
    28201 tcaatatctt gaatgattca gatgaaatga atgcgtggat cttaagtttc aaagatgttg
    28261 atccaaaaga atttgacgaa ttataaaatc agaaaatcac ttgaaaaaag ttgacactga
    28321 ttcttccagt tgctataatc ataagcaatc ctatatgaaa cgagaaagct ttgaaaagaa
    28381 gagtacatgt cacggacgtt tgaagagagc cctgtttgct gaaaatgggt aacgaaaatg
    28441 agatggaaga tggtcttaga gcagagtaag cgagcgtaag caaacttact acgggagtgc
    28501 ccgttacagc gcgacagtat acggaaagaa gctaagtcca agtactggaa gaggctgtta
    28561 ttgcgaagta acggtaaatc aaggtggtaa cacggaagtt caagctttcg tccttttgct
    28621 taaggcaaga gggcgaaggc ttttttgttt gaaaaattat aagagaaaga tgtgaatggc
    28681 atgtcattaa tccaactaac gaatgtcaaa aagacattca atgggaaaaa cggaaacatc
    28741 caagccgtca atgacgtttc actaaacgtt gaaaaagggg atatttatgg aatcgtcggc
    28801 tattctggcg caggaaaaag tacattagtc cgtctcctga atggacttga gcttcccacg
    28861 agcggcgaag tgatcgtaaa cgatcaagat attacaaaat taaaaaacaa agaattacgt
    28921 acattccgaa agaaaatcgg tatgatcttc cagcatttca acctgttatg gtcaagaact
    28981 gtacttgaaa atatccagct gccgctggaa ttagctggcg taccaaagag caaacgtaaa
    29041 gaacgggcag aagaactgtt gcgtttagtc gggttagaag gacgcggaca agcatatcct
    29101 tcacaacttt ccggcggaca gaaacaacgc gtcggtatcg cacgagcatt ggcaaatgat
    29161 cctgagatcc tgctttgtga tgaggctact agcgcattgg acccgcaaac aacagaagaa
    29221 gtattagact tattgttagc gatcaataag aaattgaatt tgaccatcgt cttgatcaca
    29281 catgaaatga atgtgatccg caaaatctgc aacaaagtcg ctgtgatgga gttggggaaa
    29341 gtcgtagaag aaggagacgt attgactgtc ttcagacaac cgaaaaaaga agtaaccaaa
    29401 cgctttgtac aacaagacat cgaaccagaa gctgattcga cagaactatt agctgaactc
    29461 atcaaagaaa atccaagcgg ccgtttggca actttgacct ttagcgaaag aaatgccaac
    29521 gagccagtca tttcccaagc gattcgacgc tataacatcg atgtcaacgt tgtgtatgga
    29581 aagatcaaac aagtgaaaga aggttccttt ggttcattga ctgtcttgat gactggtgaa
    29641 accaaagaat tagatcaagc agaagtgttc atcaaagaac aaggagtagg aattgaggtg
    29701 atccatcgtg gataaaagct tttcagaaac atatctagat tttaaacaaa tcaacccgga
    29761 aacgatgaat caggctatcc ttgatacact atatatgaca ttaatctcaa tggtcttagt
    29821 gacgatcatc ggattagttt tgggattgat cctttactct attggacgca aaaacaaacc
    29881 gtatgctcga atcatttatg gaatcgtatc gatcatcagt aatattttcc ggtcaacgcc
    29941 atttatgatc ctgatggttt tgatcattcc atttactaaa gcaatcgtag gcactatgct
    30001 aggtgcgcaa gcagccattc cagctcttgt tttatccgca gcaccatttt atgcccgtct
    30061 agtagagatt gcttttcgtg aagtcagccc tggtgtactg gaagcagcag atgcaatggg
    30121 agcaagttac tgggaaatca tctggaaggt cttgatacct gaaagtattc ccgcattgat
    30181 ttctggatta acagttacaa cgatctcgat gatcggattt actgcgatgg ccggcgcgat
    30241 cggcgcaggt ggacttggag gtcttgcttg gcaggaaggc taccagcgtg gtaatctaac
    30301 agtgacattc gttgcgactt taattatttt actaatcgta tttatcgttc aaggaatcgg
    30361 tgatttctta actaagaaaa cagacaaaag ataataggat atagggggat gtaggatatg
    30421 aaaaagaaaa tttttggatt tgcagcagcg cttttgctga ctgtaggatt agcagcatgc
    30481 ggtaacaaca gcgacagcaa agattcagca aataaagcag acgacacgac attgaaagtc
    30541 ggcgcttcac cgacaccgca cgcagagatt ttggaacatg tgaaaccatt attgaaagat
    30601 gaaggaatcg atcttgaaat cgtcaaattc gatgactatg tattaccgaa ccaatctttg
    30661 gaagaaggcg acattgatgc caattacttc cagcatattc cttatttgaa caaagaaatc
    30721 aaagaaaaag gatatgactt tgtcaatgcc ggtgctgtcc acatcgaacc aatgggcttg
    30781 tactctaaaa aagtcaaaga tgtttccgaa ttaaaagatg gtgcaacaat cattacttca
    30841 aattctgaat cagactgggg acgcatcatc acaattctac aagatgctgg cttagtcaaa
    30901 gtcaaagatg gagtagatct ggaaacagca acattcgatg atattgaaga aaaccctaag
    30961 aacttgaagt tcaaccatac gatcgaccca tcacttttag cttctacgta ccaaaatgat
    31021 gaaggtgacc tagttgcaat caacgcaaac ttcgcttacg gcgcagggtt gaatccatta
    31081 gaagatgccg tattgcttga aaaggacaac tctccttacg tgaatattat tgcgactcgt
    31141 aaaggtgatg aagacagtga caaaatcaaa aaactgattg aagtgcttca ccgcaaagaa
    31201 gtccaagaat ggatcacaga caaatggggc ggctctgtta aaccagtaaa ggctgatgca
    31261 aaataaggta tggaacaaaa aagatgagaa ataagtctgt aatggcttat ttctcatctt
    31321 ttttatacct attctaattt agaaatcaaa attgtttaat aataaataaa agatatttga
    31381 ttttagtaag gaaggtcatt gcgcttcatt ctcaattagt ctaaaatgag tgagaacgaa
    31441 agagaatttg ggggtataat gcatgtctgt cttagaaata aaaaatctac atgtgtcgat
    31501 cgaggaaaaa gaaatcctta aaggcgtaaa cctaacaatg aaaaccggag aaatccatgc
    31561 catcatggga cctaacggca caggaaaatc taccttatct gccgctatca tgggcaatcc
    31621 aaactatgaa gtaacagaag gcgaaatttt gtttgacggg aaaaatgttt tagacttgga
    31681 agtagatgaa cgtgcgcgac ttggcttatt tttagctatg caatatccaa gtgagatacc
    31741 aggtatcacg aatgcggaat tcatgcgtgc agcgatcaat gcgaaacgtg atgaaaacga
    31801 caaaatttct gttatggcat ttttgaaaaa gttagatgaa aaaatggctt tactaaatat
    31861 gccagaagaa atggctgaac gttatctgaa cgaaggtttt tctggcggag aaaagaaacg
    31921 gaatgagatc ctgcaattgt tgatgttaga acctacattt gcaattttag acgaaatcga
    31981 ttcaggtctg gatatcgatg ctttgaaagt cgttgctaaa ggagtaaatg aaatgcgcgg
    32041 agacaatttc ggtgcattga tcatcaccca ctatcaacga ttattgaact atatcacacc
    32101 tgatgtcgtt cacatcatga tggaaggccg agtagtcatg acagggaatg ctgacctagc
    32161 taaacgtctg gaagcagaag gctacgctgg tatcagtaaa gaattaggaa tcgactataa
    32221 agaagaagaa gcgtaagggg gagcaagaat gaaagaaaaa aactggctcg attatcttga
    32281 cgcggtaaat gatttttctt taagtaaagg cgaacctgat tggatgagaa ccttccgtca
    32341 agacgcacta gcgaaagcag atgaactgcc attgccgcat atcgatcggg tgaaattcca
    32401 tcgctggtcg ttatttgatg tgaaagaaac acaaaccatc agtgaaacag gcacgattcc
    32461 ggcatttgat gcaatgaaag acaatcctgt gcttgtccaa caaggttcat ggacgatttt
    32521 tgaacaactg cctgtggaat tagcagaaaa aggagtcatt tttaccgact tgttcactgc
    32581 aatgatcgaa tatccagaac tagtccaaga atattatatg aaaaaagcag taaacatgaa
    32641 cgaagaccaa ttgactgcac tccatgtagc ttttatgaac agcggtattt tcctgtatgt
    32701 gcctaaaaat gtcgtgatcg atgaaccact cgagtcctta ttcattcaag atggcgcatc
    32761 cgatgagcac ttcttcaagc atgtgttgat cgtggcagat gaacatagtg aattttctta
    32821 tctggaacgt ttccagacaa ccaaagaaca agtagcaaaa tcatcaggta atatcatcgt
    32881 ggaagtcatc gctaaagctg gttcaaaaat caaatattca gccgtagatc aattaggaga
    32941 aaatattact tcttatatga atcgccgtgg gcacatttta cgagatgctt ctgtcgactg
    33001 ggcaattggt gtcatgaatg acggtcatgt gatcgctgac tttgactcag atttagcagg
    33061 agaaggtgca cacgcagaag tcaaaatcgt ggcaatcagc tcgggcagac aaatccaagg
    33121 aatcgataca cgagtaacca ataaagcccc tcatacgatc gggcatattt tgcaacacgg
    33181 agtgatccga gaaaaaggaa cattgacctt taatggtatc ggccacatct tgaaaggggc
    33241 aaaaggtgca gatgcccaac aagaaagtcg tgtcttgatg ctttctgata aagcacgtgg
    33301 tgatgcgaat cctattcttt tgatcgatga aaacgaagta actgctggac atgctgccag
    33361 tgtaggacga gttgatccag aagaaatgta ctatctgatg agtagaggat tgcataaaga
    33421 agaagcagag cgcttagtga ttcgaggatt cctcggttct gtcttaacag caatccctgt
    33481 agaacaagtt cggaaagaat tagtcgaagt aatcgaaggg aagttgaatg gatgatcgat
    33541 gctaaaaagc tacgcaatga ttttcctatc ttatttcaag acgtcaatga tgaaccttta
    33601 gtttatctgg ataatgcagc gacgacccaa aagccaaaac aagtactcca ggcattagcg
    33661 tactattacg aacacgataa cgccaatgtt catcgcggtg tccatacgtt agcagaacgc
    33721 gcgacccatg attatgaaca agcaagagaa aaagtacggg cgttcatcca tgcaaaagaa
    33781 acagcagaag tgctgtttac tcgtggtacg acgacaagct tgaattgggt agccagaacc
    33841 ttcggcgacc gctatatcaa agcaggagac gagatagtgc tctcttacat ggaacatcat
    33901 tctaatgtta tcccgtggca gcagttggca aaacgaacag gtgctgtctt gaaatatatc
    33961 gaactaacag aagaaggctt cctggatatg gaggatgcag cgaaaaagat tacggacaaa
    34021 accaaaatcg tctccattgc tcatgtatcc aatgtcttag gtgtcttcaa tcctgttcaa
    34081 gaattagctg aacttgtcca tgaaaacggc ggggtcatcg tagtagatgg tgctcaatct
    34141 gccccgcaca tggccattga tgtccaagca ctagactgtg atttctttgc tttcagcgga
    34201 cacaaaatgt gcggaccaac aggtattggt gttttatatg gtaaacgaaa atggttagaa
    34261 gaaatggaac ctgttgaatt tggtggcgaa atgatcgatt ttgtcgaatt atacgacagt
    34321 acatggaaag aactaccttg gaaattcgaa gcagggacac caaatatcgc cggcgctatc
    34381 gcattaggcc atgcaatcga ttatttgcaa aaactcggta tggacaatat ccatcgatat
    34441 gaagaagaac tagccgctta tgtgttgcca aaattacaag caattgatgg attgactgtt
    34501 tatggtccac aagacccgaa ttttcggaca ggagtcgttg cattcaattt ggatgggtta
    34561 catccccatg atgtcgcgac agctttagac atggaagggg tagccgtcag agcaggtcat
    34621 cattgtgcgc aaccgctatt gaagtacctt gaagtatcag cgactgctcg cgcgagcttt
    34681 tatttctaca acacaacgca agacgccgac cgtttagttg aagcaatcat ggcaacaaag
    34741 gagtttttcc aacatgggac tatctaaatt agacaattta taccgtcaag tgatcttgga
    34801 tcattcttcc catccgcatc atcgtggaag cttgactgac tcgacaagca agatcgaatt
    34861 gaacaacccc acatgtggag atgtgatcga attgcaagtc gatgtaaaag acggaatcat
    34921 caaaaatatc gcctttgacg gcagtggctg ctccatcagt acggcaagtg cctcgatgat
    34981 gacagacgcc gtcatcggaa aatcaataga agaagcagaa catttaactg tagactttct
    35041 acagcttgtc caaggaaaag aagtcaaaga tcccgaccaa ttaggcgatg ctgctatgct
    35101 tggtggtgta gctaaatttc ccgctcggat caaatgtgcg acacttgcct ggaaagcact
    35161 aggaaaagca atggaaaacg atggtaccgc agctttccat cattaccaca aggaagagga
    35221 gtgagaacat gggcgtacca gaattagaag aatataaatt cgggtttcac gatgatgtcg
    35281 aaccggtttt cagtacagga gaaggcttga cagaagaagt gatccgtgag atgtcaagaa
    35341 tcaaaggcga accagaatgg atgcttgaat tccgtctgaa atctctagaa acatttaaca
    35401 aaatgccaat gcaaacatgg ggccccgacc tttcagacat cgatttcgat gccatcaaat
    35461 attaccaaaa accaagtgac aaacccgctc gtgactggga agacgtacca gaaaaaatca
    35521 aagatacatt tgaaaagatt ggtataccag aagctgaacg agcttatcta gccggtgctt
    35581 ccgcacaata tgaatcagaa gtcgtgtacc ataatatgaa agatgaattc gaaaagttag
    35641 gaatcgtctt tactgacact gactctgctt tgaaagaata ccctgaatta ttcaaagagt
    35701 acttttcaaa acttgtaccg ccaaccgaca acaaattagc agcgctgaat tcagccgtat
    35761 ggtcaggtgg gacctttatt tacgtgccaa aaggcgtcaa agtcgatgtt ccattacaaa
    35821 cgtacttccg gatcaatgcg gaaaacacgg ggcaattcga acgtacgctg atcattgttg
    35881 acgaaggagc aagcatccac tatgtcgaag gttgtacggc acctacttat tcaagtaaca
    35941 gcctgcatgc cgccatcgta gaaatcttta caagaaaaga tgcgtattgc cgttacacaa
    36001 cgatccaaaa ctggtcagac aatgtgtaca acttagtaac caaacgagca aaagcatacg
    36061 aaggcgcaac cgttgaatgg atcgacggaa acctaggtgc aaaaacaacg atgaaatacc
    36121 caagtgttta cttagatgga aaaggtgcac gaggcaccat gctctccatc gcctttgcag
    36181 gagcaaacca aatccaagat acgggtgcaa aaatgatcca caatgcacca aatacatcta
    36241 gctctatcgt ctctaaatca atttcaaaag acggcggaga agtaaactat cgaggacaag
    36301 taacctttgc caaaaacagt gcgggttcga tctctcatat cgaatgtgac acgatcatca
    36361 tggacgaatg gtcgaaatcc gatacgatcc cattcaatga gatccacaac agccaagtct
    36421 ctttagaaca cgaagccaaa gtctcaaaaa tctcagaaga acaactctac tatctgatga
    36481 gccgaggcct gaccgaatct gaagcaactg aaatgatcgt catgggcttt gttgaaccat
    36541 tcaccaaaga acttccaatg gaatatgcag tcgaactcaa tcgattgatc agttatgaga
    36601 tggaagggtc tgtaggttaa taactatttc attgctcgaa acgttgatat taaagcgttt
    36661 cgagcgtttt tttatttgta ttttaatggt tggctacgtt tttggctacg tttggaatta
    36721 ttcgcccata aattttgcaa atcggtcacc agttttctta acagattgtg gtgtgacatg
    36781 agcatagata ttcatggtag ttttaatatc tttatgacct aatcgttcct gtacttcttt
    36841 tatcgacgca ccactttcaa acaataagct acagtgggta tgcctaaatc catgtggagt
    36901 aatttgaggc tccatcggtg tctttttgta aatccagtct aaccaatcgt ttactacttg
    36961 ggggtagtac aacttgtttg tgtttgtagt gaaaagaaac tgttttggtg aattcgtgtt
    37021 atagccatat aagatcatta ttttcatttg ttcttgttgc caaatacgca gttgtttaag
    37081 tgtaaaatca tcaagctgaa tcgttcttga tgaattttta gttttaggta cttgtagaac
    37141 tacttgatta aactcgtctt gagcgatagt tttaccaatt gttattgttt tattgaaata
    37201 gtctatatct tcccattgaa gcgccaatgc ttcgctttta cgcattcctg tataagctaa
    37261 taaacggaaa aaagtgtaca gtttaactga attattttct ttagtattct gtaaaaacag
    37321 ttgtaattga tctttcgtgt aaaacttcaa gggttgttct tcttcaattt ttcttggaag
    37381 caatgtttta gacataggat tatccttaca gacttccatt gctactccaa atttaaatat
    37441 ttgagcagtt gtttttctca agaaaccata ttgttttaat gttttgtgcc attcattcac
    37501 ctgtttttgg caatagctaa cacttatttt atccaatcgt aattccccaa aagcaggtaa
    37561 tatatggttt ttgcaaaaac gttgagcaac ggcaacagaa gacggtttta ctcttgttct
    37621 gtagttatca atccataatt catacaactc ttgaaaagtt gtaacattga ttttaaactc
    37681 ttgtttttct ttaaagtcta caagtagtct attaagtgct gtttgcgcct cagcttttga
    37741 tttgaatctt ctacgctttg tacgtatatc ttttccagtc ttgggatctt tccctaaata
    37801 agtttcaaat ccccaatatt tttcgccatt ttgtaattta taactaaaaa atgttgccat
    37861 ttgattctct ccttacttca ttattgcagg tagagaagtt atttgaagaa tcaattattt
    37921 ttaaatttat cgtgattatc agttaaaaat tttgcgatta tactattttt ctcatatggg
    37981 aaattgtatt ttttactaag atctgaaata aatttttctg tttcaaattg gtttttagta
    38041 attttataaa agaaatcctc gacagatgaa tactttgaaa tatctttata ggctaagtcc
    38101 atttcgtcta atagctgttt taaagaatca atcattcttt ctttttcacc gtatttgaaa
    38161 tcaccatttc ttatcttgtc ttgaagttta ctgttggctc ttttgaaaaa tagctcttta
    38221 gagatagagt gtctttggtt agcgtactct tcaatcagag gattgatatt attagcaagt
    38281 atctcattta gataaatttc aaaatctttt gagtaatatt ttgtaaatat attttttatt
    38341 ataggattca ataactcata gtctttatct agcgaaaacg tatcactata tctttcttga
    38401 atgtctttaa agactttgtg aggtatttct gggaagtcct taatatatag ctcataccct
    38461 atatcgataa ggtatgaagt tatatattct tctaaagttc cccatttaat ccagtcaatt
    38521 gttgaattag tgatagttga taataatttc agcttttgtg gatttggtaa attggtccca
    38581 cgttcccagt tggatatggt cgcacgtggt gaattatcta ttttccttcc aagttcttcc
    38641 atagtaaaac cacatgaaag gcgtaattgt ttgattcgtt ttcctgcttc tgaaggattt
    38701 ggcttcatct tttttctcct ttttattttt aaagatagta tatcatattt ataaaataaa
    38761 agtatttaaa agtatttact ttttatctgg agtatgatac tttattctta taaatacaaa
    38821 taaatacatt tttttacgca atttgtattt atttgtactt ataaaggtag aggagttata
    38881 gaataattaa ttaaaaagga gaggtttttg tggaagcaat aaaattaatg atttcagaag
    38941 aacaggagga aatgtttcta aagcagtttt atcaaattgc tacaaaagct attgaaaagg
    39001 ctcaagaaaa tgcggggatg acaagggaat atctcaatca aaaagaaatg gcggaatact
    39061 taaatgtgag tgaaaacttt ttaaaaaatg ctgtgagaaa tgagggattg cccgtagttg
    39121 aattaggggc aagaaaattt tattcgaaaa aggcagttaa tgagtttatg tttgctcgaa
    39181 tgaaaagagg ggaaaatatt ggaagatgat gaagtagaaa ttataaaaaa atgctttaaa
    39241 aaaagaaaaa aagtgttccc aaaagtttgc agactggggg aacactcaat aataaagtgc
    39301 tgagaaaact ttatttatag ctattatatc aaaaaagaag gtgaaaaggt gttttcttct
    39361 ggaaatgttg tagttgacaa acttagagaa ataaattttg acggaaatgt aatccacaca
    39421 catggtacga tcaattaaga aaaaaaacga aaaaaggtgt tgagaagccc tatttgaatg
    39481 caatagttat cttagcagaa attacctatt ggtatagacc taaaaaaatt tttaacgatg
    39541 atggtcagct acttggttat caaaaaaagt ttcttgaaga tattttgcaa aaatcatata
    39601 agcaattaag caaaaaaaca ggattatcag aacgagctat aaaagatgca attgtgtact
    39661 tagagaaaaa ggggattatc aaaagggttt ttagaaatat ttattatgag aaaagagcag
    39721 ttacattatc aaatgttatg tacattgttt taaatgtaca taaattgata gaaattactt
    39781 ttcctacttt ggattgcgaa aaaaaggagt atatgccgaa aattcgtcag aggggtatcg
    39841 aaaaaaagag atatgtctct ttaaaaaaag gtagaggtat gtacaaattt gtatatacgt
    39901 atacaaagac tacttcacag aacatatcaa agactactct cgttatggaa gaagaggata
    39961 ttaatataaa gaggatttac agtaagatta gtgttcttta tgacgcaaat aacgaagatg
    40021 gtcgaaaatt atttcaagtg tatcactatt tgagaaacgc taatgtagat acagaagata
    40081 tcagaaaaat cattgaatat ttatctgaaa atactcagta tcttattgct gagtgtatta
    40141 taaaacaaca tgaagcatgc ttttttcaaa gaaaaactga agaagggtta tacgattatg
    40201 taacatattt tttgaatgga cttcagaaaa gatataaaag tcaattttta gaaaatgtag
    40261 agcctataga tcagttcaaa aaagctttcg aaatcaaaga gttacctgaa attactatgc
    40321 ataattggtt gaaaggagaa gaatcatgat taaaaatcgc ttaatagatc aaatgtggtg
    40381 tcttgatcaa aatacagata ctctcgaatt aatcgagagt atctgcttca acacagtagt
    40441 tctcgattta agagctgaaa atgacaattg tgtaactcat atcataatta atcaaaaaat
    40501 ggctcagcag ctctctgaga gcctaagaaa atgggctgaa ggagaaaact atgaaagcaa
    40561 ttaaagaaaa aatcgttagt agaaaaataa actggttagc actcagtgta cttgttttaa
    40621 tgattagcgt tgtttttaca agtaatattg ttgaagctag cattttcaat tggtttgatg
    40681 acacaaaaag tcaaacagag tttttgaata attctcttta tagaccttac ctaaaaaaat
    40741 acaatggaga tttccttagt cagttcggga tttggcttgg atgggctgtg gtaaaaggca
    40801 tgtttactgt gactgattcg attcaaaata tgattccaga tgtgttggat ttgttcaatt
    40861 tcattgaaag tacagggcta aataatgttt atcaatcagt cataaataca attgtcgttg
    40921 gattaatgat cctttcattg atgtttgttg gctacaagat gattactgga aaaggaacaa
    40981 tagatttaag atctgttgga atgaatatag tgatgtctgt agcgttaatt cttttaatgc
    41041 caacaatgat ttctagcggt attcaatttg ccaaaatatt ttacaatgat gctactacga
    41101 ttacaaattc agatgacgga gtcgcttggt cattgataaa acaaggagta acagatctgg
    41161 catatattaa taaaacagat caatactcaa gcattgataa aacggaggat cgaaataagt
    41221 taacacgaaa aaattttcaa cagacagatt taacacaagt gttgacggat aaagtgattg
    41281 ataaattgga aaaagaaaat ccagctgcgg ataatcttcg ctatgaatta gttgaaaatt
    41341 ctaataatga atttgttgcc acaaagtttt ctgataactt cttatcaaca ttttctgata
    41401 gcttgaaatc aggttattat cgatatcaag caaatctttg ggggatttcg attgggttga
    41461 cagcgttggc aatcgcctat gtgttcagcg cgtttgtgat cattacagca atattagaac
    41521 ttgcatttaa acgtgtgtta ggtgttcttg tttttgcgac agatattgaa acaggacaac
    41581 gttcaaaagt tgttttatcc gatattttac aatgttactt aacagttgga tttcaagggt
    41641 ttggactatc tatgtttgct atgtttatca attttttaaa tgctggacaa ggtatctcca
    41701 caaatatttt tataaaaaca attgcttata tctgtgctgt gtttgtgctg ataaaaggca
    41761 gtggaacagt catgagatac tttggtgttg atattggact caaagaaggc tatgggcaac
    41821 ttgcttctgc ttttggtatg ggcgcaatgc tgtttagaaa aggatcaact ggatttaatc
    41881 gtgctaaagg aagcggaaat ggaaatggat cagacagtgg agaaggagaa gatcgaaaac
    41941 cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg caaaactgga agagctttgg
    42001 gatatgccca tgaacgtgga ttatcaggtt tagcttcaga tggagctaca atggctagtg
    42061 aaagagcaat aaaaccattt aagagtatgc gtgatatggc taatgatacc aagaataagt
    42121 tcaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc aaacctatgt
    42181 tagctaaaaa taaagaagat gaaactggaa aatatgctga ttccatgcca acacgtcttt
    42241 cggatagaaa agatggtgct aaggttgaaa atgccgatag aattatgtct agttctgaac
    42301 gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgta agtgcaattc
    42361 agcaaaaagt tcaacaagat attgaagaac gtaaaaatgc tatgcacggt caagctaagt
    42421 ctgctgaaga attaattaat cagaagcgac aagaagctaa atacacgcct gaagcaatga
    42481 atcgtgagga aatgttaagg aaacgtgtag aagggcgtac aggagctaat acggacgaaa
    42541 aagaagcgtt gaccaaagaa agaatccaag aagctaaaaa ctcaaatacc ggattagaaa
    42601 aattggtcaa agaaaatcta caaaattcta caagtagtca aggtggccga tcagtagatg
    42661 tccgagaaaa tattcaaggt tcattgaatg gtcatgtcgg acggaatgtt gatgtaagag
    42721 agaatttaca aagctcttca aatggtcaag gtactaagac aattgatgtt cgagaaaatg
    42781 tacaaaattc tacaagtagt caagggttta aatcaatgga tgtaagagag aatgtacaaa
    42841 attctacaag tagtcaaggg tttaaatcaa tggatgtaag agagaatgta caaagttcta
    42901 caagtagtca aggattcaaa acagtagatg ttcgggagaa cgttcaaaaa gatagtggag
    42961 aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca gcgaaaaaat
    43021 ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat gataagggat
    43081 caaaaccaag acgaagattt acctatgaag acaacgagtt attcagagat acacttaatg
    43141 atcctaatcc gcttttcgat aatctattaa aaaaataaaa gggggaattg tcatgaaatt
    43201 aattctaaag attagtacgc atgatttctt agaacttgac gaattatgtg aatcagtaaa
    43261 gaacgcaaca aatgaaaaaa tcacggtcac taattatatt gaactgatga ttcaagaaga
    43321 attaaagatg aaacatgaga taaaaaacaa cttgaaatat tgttatctag aaaaaagtga
    43381 a
//
LOCUS       DAZUMO010000020        43069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000020 DAZUMO010000000
VERSION     DAZUMO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.020"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            600..1565
                     /locus_tag="ACG8ER_001048"
     CDS             600..1565
                     /locus_tag="ACG8ER_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFS5049647.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            1587..1799
                     /locus_tag="ACG8ER_001049"
     CDS             1587..1799
                     /locus_tag="ACG8ER_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5049648.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            1874..2350
                     /locus_tag="ACG8ER_001050"
     CDS             1874..2350
                     /locus_tag="ACG8ER_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFS5049649.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            2347..4155
                     /locus_tag="ACG8ER_001051"
     CDS             2347..4155
                     /locus_tag="ACG8ER_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS5049650.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            4232..4447
                     /locus_tag="ACG8ER_001052"
     CDS             4232..4447
                     /locus_tag="ACG8ER_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFS5049651.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            4539..5402
                     /locus_tag="ACG8ER_001053"
     CDS             4539..5402
                     /locus_tag="ACG8ER_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFS5049652.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            5439..5975
                     /locus_tag="ACG8ER_001054"
     CDS             5439..5975
                     /locus_tag="ACG8ER_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS5049653.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            5989..6411
                     /locus_tag="ACG8ER_001055"
     CDS             5989..6411
                     /locus_tag="ACG8ER_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFS5049654.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            6341..8740
                     /locus_tag="ACG8ER_001056"
     CDS             6341..8740
                     /locus_tag="ACG8ER_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFS5049655.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            8772..10763
                     /locus_tag="ACG8ER_001057"
     CDS             8772..10763
                     /locus_tag="ACG8ER_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS5049656.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            10786..11037
                     /locus_tag="ACG8ER_001058"
     CDS             10786..11037
                     /locus_tag="ACG8ER_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS5049657.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            11027..12256
                     /locus_tag="ACG8ER_001059"
     CDS             11027..12256
                     /locus_tag="ACG8ER_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS5049658.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            12253..14334
                     /locus_tag="ACG8ER_001060"
     CDS             12253..14334
                     /locus_tag="ACG8ER_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS5049659.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            14483..18403
                     /locus_tag="ACG8ER_001061"
     CDS             14483..18403
                     /locus_tag="ACG8ER_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS5049660.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLPNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            18404..19348
                     /locus_tag="ACG8ER_001062"
     CDS             18404..19348
                     /locus_tag="ACG8ER_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049661.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            19352..19555
                     /locus_tag="ACG8ER_001063"
     CDS             19352..19555
                     /locus_tag="ACG8ER_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS5049662.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(19855..20091)
                     /locus_tag="ACG8ER_001064"
     CDS             complement(19855..20091)
                     /locus_tag="ACG8ER_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049663.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(20274..20645)
                     /locus_tag="ACG8ER_001065"
     CDS             complement(20274..20645)
                     /locus_tag="ACG8ER_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS5049664.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(20642..21970)
                     /locus_tag="ACG8ER_001066"
     CDS             complement(20642..21970)
                     /locus_tag="ACG8ER_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5049665.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(21931..22260)
                     /gene="mobC"
                     /locus_tag="ACG8ER_001067"
     CDS             complement(21931..22260)
                     /gene="mobC"
                     /locus_tag="ACG8ER_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS5049666.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(22518..22889)
                     /locus_tag="ACG8ER_001068"
     CDS             complement(22518..22889)
                     /locus_tag="ACG8ER_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5049667.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            23034..23219
                     /locus_tag="ACG8ER_001069"
     CDS             23034..23219
                     /locus_tag="ACG8ER_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5049668.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            23377..23544
                     /locus_tag="ACG8ER_001070"
     CDS             23377..23544
                     /locus_tag="ACG8ER_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049669.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            24162..24829
                     /gene="vanR-B"
                     /locus_tag="ACG8ER_001071"
                     /pseudo
     CDS             24162..24829
                     /gene="vanR-B"
                     /locus_tag="ACG8ER_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
     gene            24832..26175
                     /gene="vanS-B"
                     /locus_tag="ACG8ER_001072"
     CDS             24832..26175
                     /gene="vanS-B"
                     /locus_tag="ACG8ER_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS5049670.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            26346..27152
                     /locus_tag="ACG8ER_001073"
     CDS             26346..27152
                     /locus_tag="ACG8ER_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFS5049671.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            27170..27997
                     /gene="vanW-B"
                     /locus_tag="ACG8ER_001074"
     CDS             27170..27997
                     /gene="vanW-B"
                     /locus_tag="ACG8ER_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS5049672.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            27994..28965
                     /locus_tag="ACG8ER_001075"
     CDS             27994..28965
                     /locus_tag="ACG8ER_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS5049673.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            28958..29986
                     /gene="vanB"
                     /locus_tag="ACG8ER_001076"
     CDS             28958..29986
                     /gene="vanB"
                     /locus_tag="ACG8ER_001076"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS5049674.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            29992..30600
                     /locus_tag="ACG8ER_001077"
     CDS             29992..30600
                     /locus_tag="ACG8ER_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS5049675.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(30852..31856)
                     /locus_tag="ACG8ER_001078"
     CDS             complement(30852..31856)
                     /locus_tag="ACG8ER_001078"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5049676.1"
                     /translation="MQRGGKSRGKAAAAGSGKQKASNGERFLKKIERTGGEAVNRLTR
                     RRKVYEAIHTLRQEKGYEIASLCALSGIPRCSYYKWLHREESASEKENTLLLEEIIRL
                     YSEVKGIYGYRRMTLNVNSRLKRNYNHKRIYRLMKSVHMQAVIRRKKKHYVILEPRVT
                     AENVLNREFTANGPNEKWLTDVTEFKLINGKKAYLSAILDLHDKSIVAYALGQSNNNQ
                     LVFDTFDRAVRANPGAHPLFHSDRGYQYTNRQFKAKLDGIHATQSMSRPGRCIDNGPM
                     EGFWGILKSEMYYLRQFHTFEELKKAIDEYIDFYNTRRLQAKLKGLAPLAYRNQTLVA
                     "
     gene            complement(31739..32422)
                     /locus_tag="ACG8ER_001079"
     CDS             complement(31739..32422)
                     /locus_tag="ACG8ER_001079"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5049677.1"
                     /translation="MSLKGKIPPEVKIQAVEDYLAIRKGSTQIREELGIRLSTFQAWL
                     RKYQTEGPAGLHPKKHVTSYPSSLKLAAVEDYMGGGGSLDAMCRKYGISSHAVLQQWI
                     TLYNQGHREFKTRRAREETDMAEKEKLSQEQKLQAVLYCLEHQLDYRRTSEQYKITYQ
                     QIYSWVKKYQEQGEAGLLDRRGRRRPPSEYSEEEKAAAKLRQLEAENRRLQMENDFLK
                     KLNELEGRR"
     gene            32868..33299
                     /locus_tag="ACG8ER_001080"
     CDS             32868..33299
                     /locus_tag="ACG8ER_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5049678.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            33306..33539
                     /locus_tag="ACG8ER_001081"
     CDS             33306..33539
                     /locus_tag="ACG8ER_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5049679.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            33963..34163
                     /locus_tag="ACG8ER_001082"
     CDS             33963..34163
                     /locus_tag="ACG8ER_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS5049680.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            34247..35440
                     /locus_tag="ACG8ER_001083"
     CDS             34247..35440
                     /locus_tag="ACG8ER_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5049681.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(35619..35897)
                     /locus_tag="ACG8ER_001084"
     CDS             complement(35619..35897)
                     /locus_tag="ACG8ER_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049682.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFF"
     gene            complement(36015..36275)
                     /locus_tag="ACG8ER_001085"
     CDS             complement(36015..36275)
                     /locus_tag="ACG8ER_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049683.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(36412..36825)
                     /locus_tag="ACG8ER_001086"
     CDS             complement(36412..36825)
                     /locus_tag="ACG8ER_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS5049684.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(37281..37766)
                     /locus_tag="ACG8ER_001087"
     CDS             complement(37281..37766)
                     /locus_tag="ACG8ER_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049685.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(37905..38120)
                     /locus_tag="ACG8ER_001088"
     CDS             complement(37905..38120)
                     /locus_tag="ACG8ER_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS5049686.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            38344..39144
                     /locus_tag="ACG8ER_001089"
     CDS             38344..39144
                     /locus_tag="ACG8ER_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5049687.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            39163..41031
                     /locus_tag="ACG8ER_001090"
     CDS             39163..41031
                     /locus_tag="ACG8ER_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5049688.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            41024..41515
                     /locus_tag="ACG8ER_001091"
     CDS             41024..41515
                     /locus_tag="ACG8ER_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS5049689.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            41503..42057
                     /locus_tag="ACG8ER_001092"
     CDS             41503..42057
                     /locus_tag="ACG8ER_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS5049690.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            42075..>43069
                     /locus_tag="ACG8ER_001093"
     CDS             42075..>43069
                     /locus_tag="ACG8ER_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS5049691.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT        11631 a        10312 c        11033 g        10093 t
ORIGIN      
        1 gattgatttg agatcttttt atacttcaaa aaagataaca cccaactttc aaaaatatgt
       61 tttggaagtt gggacaaacg agttaataca ttttaataca ttatttttta ttcaaaaatt
      121 ttaggttatc gctgaaaacc ccggaaatac gggcatttcg caggatatga gaactcaatt
      181 tactaccaat ttactacttt cggattgagc cgcatatatg ctgaaccata ctgctaatac
      241 ccgccacccc aaatacggca catcataaat tgaatacgac accgtaaacg cggcgttcct
      301 ctttcccgtc atgggagaac aggacgccgc ttttttatgc ccaaatagag aaaggaggcg
      361 acgccatgct atttacttcc ggccctgacc gggcgtttga acggctgatg caggaaaagc
      421 ccggtttcga tcactacgac ggcggcgggg cggacgcgcc ggaggattgc ggcacctgcc
      481 gattttaccg cccggattgg aaatatcaat tctgccagtt tgcagagtgt ccctatcagc
      541 cgggcaagct gaccgactat gcagcggtca aattcacagt gaaaggagcg aacaacgcta
      601 tggcagtttt ccgtgtggaa aagaacaaag gctatacggt gatgtcaaat caccacctgc
      661 ggaataagaa cctgacctta aaggccaagg gcctgctgtc gcagatgttg tccttgccgg
      721 aggattggga ctatacccta aaaggactgt cctatatcaa ccgggaaagc atcgacgcga
      781 tcaggacagc ggtatgggag cttgaaaaag ctggatatat ccggcgtgag cagggccgtg
      841 acgcaaaagg caaaatggcc gatatggtct ataccatcta cgaacagccg gtattggaaa
      901 acccggtatt ggaaaatcca acatcggata acccggtgtt ggaaaatccg acatcggata
      961 atccggcgtc ggaaaatcca acgcaattaa ataaagaagt acaaagaact gacttatcaa
     1021 aaaaagaaaa aataattaca gatggacaaa gtacccattc cattcctatc ccatccccga
     1081 cccctgcccc tatggagggc gaggcagcgg ctacgccgcc ggaacggaaa aggacaggaa
     1141 aggatgaggc tgtacggatt tatcgggaaa tcattttgga gaacatcgag tatgcctatc
     1201 tcattcagga caggagcatt gaccgtgaac agcttgacga aatcgtagac ctgatgctgg
     1261 aaaccgtctg caccgcccga aagacaatcc gtgtcgccgg ggacgactac cctgccgagc
     1321 tggtgaaatc caagtacatg aagctgaccg gcgaacatat ccgctttgtc cttgactgtc
     1381 tgcgggagaa cacaacgaaa gtccgcaata tcaaacaata cctgcgggct atgctgttca
     1441 acgcccccag caccatttcc aactactaca cggcccttgt cgcgcacgac atggcacagc
     1501 ccgattgggg aaagcctaaa tccggcctgc cggactattc ctgcggcgag ggcgaaagcc
     1561 tgtaacgaga aaaggaggac gaccctatga agcagggcgc attgattttt gacgagcgaa
     1621 cagaccgtta cgacatccgc tttgatctgg ccgactatta cggcggcctg cattgcgggg
     1681 agtgtttcga cgtgatgatc ggcggcaaat ggaggcccac ccgcattgaa atggccgaaa
     1741 actggtatct tgtcggcatc cgggccgacg acctttccgg cctgcgggtg cggatttgac
     1801 ctatatctac ctgcggcgtc tggtggttcc gccttagacg ccgcttttga ctttctgaca
     1861 aggagggatg cgattgcagg aagaagttac tcaaaagacc attgccctgt ccatgaaaac
     1921 gggcaagctc acggctcaag tgctgcaagc ggcgctgaaa aagtacctgc aacaccgggc
     1981 caagggcaaa aacacgctgc accacggcca gcagagccta aagcagctta aaaagcatgg
     2041 ggctgatctc tccaacattg agatcaccga ggccaacatt ggagcgttca agccatgcgc
     2101 caagaaatac ggcgtggatt ttaccttacg aaaagataag accacccagc cgccccacta
     2161 cgttgtgatt ttcaaggcca aggatgcaga taaccttgat ctggcgtttc gggagtttac
     2221 cgccaagacg ctttccaagg agcagcggcc ctctatccgc aaggttttgt ccgctgtgaa
     2281 gcaaaaggcg gcggcccaga ccaagcagcg ggccaaggag aaaatcaagg aaagggggct
     2341 ggaactgtga agcctgacgt aaagaaattg ctgatcctga acctcccgta tctgattttt
     2401 gtgtacctgt ttgcgaaatg cggggaggcg taccggctgg ccgctggtgc tgacctgtcg
     2461 gggaagctcc tgcattttgc ggatggtttt gcggcggctt ttgccaaccc gctgccaagc
     2521 ctgcacctgt ttgacctgtg tatcggtatc gccggtgccg tgatcgtgcg gctggtggtg
     2581 tactgcaaga gcaagaacgt gaagaaatac cgcaaaggca tggagtatgg cagcgcccgc
     2641 tgggggacgg ccaaagacat agcgccttac atcgacccca agtttgaaaa taatatcctg
     2701 ctcacccaaa cggaacggct taccatgacc ggcaggccca aagaccccaa gacagcgcgg
     2761 aataaaaacg tgctggtgat cggtggcagc ggttcaggga aaacgagatt tttcgtgaaa
     2821 cccaacctga tgcaatgctt tccgaccacg gactatccga cttcgtttgt ggtgacagat
     2881 ccgaaaggca ctttggttct ggaaaccggc aggatgttcc agcaggcggg ctaccgcatt
     2941 aaaatcctga ataccattaa cttttccaag tccatgaagt acaacccctt tgtctacatc
     3001 cattcggaaa aggacgtgct aaagctggta aataccctga tttccaacac caagggcgag
     3061 ggggaaaagt cagcggagga tttttgggtg aagtcggaac ggttgtttta ttcggccctg
     3121 atcggctata tctggtacga ggccccagcg gaggaaatga acttctcaac gctgctggaa
     3181 atgataaacg ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc cgtcgatctc
     3241 atgtttgagc gtttggagca gaaagacccc gaccattttg cggtgcgcca gtacaagaaa
     3301 ttcctgctgt ccgcaggcaa gacccgttct tccatcctga tttcatgcgg tgccagactt
     3361 gcgccctttg acatccggga ggtacgcgag ctgatggagg acgacgaaat ggagctggac
     3421 accatcgggg acgaaaagac ggtgctgtat ctcattatga gcgatacgga cacgaccttt
     3481 aatttcattc tggctatgct ccaaagccag ctaatcaacc tgctttgtga ccgggctgat
     3541 gatgtgtacg gcgggcggct gcctgtccat gtacgcctga ttttggatga atttgccaac
     3601 attggccaaa tacccaattt cgataaactc attgccacca ttcgaagccg tgaaatatcg
     3661 gcctctatta ttttgcagag ccagtcgcag ctaaaagcga tttacaagga cgccgccgag
     3721 atcatttcgg ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa aaatgccaag
     3781 gagatttccg atgcgctggg gaaagaaacg attgacagtt tcaacaccag cgagaaccgg
     3841 ggaagccaaa cgtcccacgg attgaactat caaaaattag gaaaggagtt gatgtcccaa
     3901 gacgaaattg ccgtgatgga cggcggcaag tgtatcttgc agctacgggg tgtgcgcccg
     3961 tttttatcgg agaaattcga tattaccaag catccccggt acaaatacct tgccgacgcc
     4021 gacaagaaga atacctttga cgtggacaga tttttgacta tcacgcgccg gaaacggcag
     4081 caagtggtaa cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc ggacgaggac
     4141 gccgccgctg aataaacagc ggcttttttc attccaacaa caaaatcaaa aaatcattat
     4201 gagccggaaa ccggcagaaa gaggtaaatt tatggcattt tttaattccg cagttactac
     4261 tttgcagact atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg gcatcaatct
     4321 gttggaggga tacgggcagg acaatccggg ggcaaaatcg cagggtgtca agcagcttgt
     4381 cgctggcggc ggcgtggccc tgatcggtgt cactctggtt cccctgctgt ccgggctgct
     4441 gggctgatcc tggcctttgt ccctgtccaa acccttaact aatcgcctcc gcgtcctccc
     4501 ggccagaccg ggagggcgcg ggaaaggagg tgctacccgt gattagtgaa atcattgaag
     4561 aatggataaa aggcatcctc atggacggta tcacgggcaa cctgtccggg ctgttcgata
     4621 cggtaaatgc caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg caggcatgga
     4681 acagcggtat tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc cccatagccg
     4741 gggccatact cgcccttgtc atgtgctatg agcttatcca gatgataacg gaaaagaaca
     4801 atatgcacga ctttgacagc tccatgttct tccgctggat attcaaatcc gcgtttgcaa
     4861 tcctgatcgt aacgaacaca tggaacatcg tcatgggcgt attcgacgcc acccaaagcg
     4921 tcgtcaatca aagcgccggt gtgattatcg ggcagacatc cattgatttt aacaccctga
     4981 taccagacct tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg gggctgtggt
     5041 tccaaaccct tgttgtgggc ctcaccatga atatcctttc catctgcatt ttccttgtga
     5101 cctatggccg catggtggaa atctatgttg tcacgtcgct ggggcctatc ccccttgcca
     5161 caatgggcaa cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc ctgctggcgc
     5221 tgggcttcca agccttttta atcatggttg tggtgggtat ctacgcggtg ctgatacaga
     5281 atatcggttc cgcttccgac atatccggcg caatctgggg ctgtatgggc tatacggtgc
     5341 tgctgtgctt ctgcctgttc aagaccggca gtatatcaaa ggccgtattc ggggcgcatt
     5401 gatcccaaaa tccaagcata cacaggagga acttgtttat ttcaacgtac aacatcattt
     5461 acgctgaccc gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg gaaaatcact
     5521 attccaccat gagcattgac gagctgtgcg ccctgccggt ggaacggctg gcgtccaaag
     5581 actgcctgct gtttttgtgg gccacattcc ccatgctgcc ggaagctctg cggttaatca
     5641 aagcgtgggg cttttcattc aagaccgtgg cttttgtatg gctgaaacag aaccgtaaaa
     5701 gccttacatg gttttacggg ctgggccgct ggacaagggg aaacgcggaa atctgcctgc
     5761 tggcaacaca cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt attatcagcc
     5821 cgattgaaca acacagcaaa aagccggatg tgacccgtga aaaaatcgta gagctggcgg
     5881 gcgatttgcc ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg gacgtgtggg
     5941 ggaacgaggt ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat ggcttatgta
     6001 accgtcccca aggacttaac ccatgtgaaa agcaaggttg tgtttgggct taccaaacgg
     6061 caactgatct gtttttccgg ggcgctggtg atcggcgtcc ccttatattt tctcacccgt
     6121 gactatctta ccaacagcgc ggcggccctg ctcatggtat ttgccatgct gccgggcttt
     6181 ttgcttgcca tgtacgagcg tcacggccag ccccttgaag tggtgattat gcagatcatc
     6241 cagtgctgct tcatccagcc aaaggagcgt ccctatcaga ccgacaacac ctacaccgcc
     6301 cttgtgcggc aataccagat ggaacaggag gtaaaggcca ttgtccaaaa aggaaatccc
     6361 aagagaaacc gaaaaaacaa agctgacccg cgcccagcga aaagaaattg acgcggtaat
     6421 ccgtaaatat aagggcgacg gcaggcccca cacggcgcag cagagcatcc cctacgaggt
     6481 catgtacccg gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt gcattgagtt
     6541 tgcggacatc agctatcagc ttgcccagcc ggacacgcag acggctatct ttgaaaaact
     6601 gtgcgacctg tataactatg tggacgcctc cattcatatc cagttttctt tcctcaatcg
     6661 gaaagttgac ccggtgcagt acgccaagag ctttgagatc gcgccgcagg gggacgactt
     6721 cgacgacatc cgggccgagt acaccggcat tctgcaaaag cagcttgcaa acggcaacaa
     6781 cggcatggta aagaccaaat acctgacatt caccatcgag gccgaaagcg tgaaagcggc
     6841 gcgagccaga ttgaagcgca tcggctttga cctgttaggc tatttcaaga gcatgggggc
     6901 cgtagcccat gtgatggacg gctgggaacg gctgaacctg ctgcatggcg tgtaccaccc
     6961 ggacggcgag atattcaatt ttgactggaa gtggctggcc ccgtccggcc tctccaccaa
     7021 ggattttatt gccccgtcct ccctgtgctt cggcaatgca aagacattcg gcatgggcgg
     7081 caaatacggc gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg acgatatgct
     7141 ggccgacttc ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc aggccattga
     7201 acagaccaag gcgatcaaga ccatcaagcg caaaattacc gaccttgacg ctatgaagat
     7261 tgcggagcag aaaaaggccg tccgcagcgg ttacgatatg gacattcttc ccagcgacct
     7321 tgccacctac ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc gcaatgaacg
     7381 gctgttccag ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa agctgaataa
     7441 cgacgtgttc caagccgcag gcgtggccca aaagcataac tgtccccttg tccggctgga
     7501 ctaccagcag gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc agatcaagat
     7561 acagcgcagc cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga cgcaggagct
     7621 ttttcagggc ggcgcggcca tgtattacgg catcaacgcc aagtcccgca acatgattat
     7681 gctggacagg aagcaggccc gttgccccaa tgccttgaag ctcggcaccc ccggcagcgg
     7741 caaatccatg agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc ctgacgatat
     7801 ttttatttca gacccggagg ccgaatatta cccccttgtc aaacggctcc acggacaggt
     7861 aatccggctg tcgcctacca gcaaggactt tgtgaacccg ctggacatca acctgaatta
     7921 ttcggaggac gataacccgc tggccctgaa atccgacttt gtgctgtcgt tctgtgagct
     7981 tgtcatgggc ggcaagaacg ggctggaagc cattgaaaag accgtgatag accgggctgt
     8041 gcgcgtgatc taccggccct atctggcaga cccccgcccg gagaatatgc cgatcctgtc
     8101 cgacctccac aaagcgttgt tagatcagca tgtcccggag gctgaccggg tggcgcaggc
     8161 ccttgacctg tatgtgtccg gctccctgaa cgtatttaac caccgtacca acgtggacat
     8221 cggcaaccgg cttgtgtcct ttgacattaa ggagctgggc aagcagctca aaaagctggg
     8281 gatgctcatt gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa gccaaggaaa
     8341 ggccacatgg tattttgccg acgagttcca tttgcttttg aaagaggaac agacggcggc
     8401 ctattctgcg gagatttgga agcggtttag gaaatggggc ggcattccaa caggtgccac
     8461 ccagaacgtc aaagacctgc tttcctcccc ggaaattgaa aatatccttg aaaacagcga
     8521 cttcattacg ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg aacgcttgaa
     8581 tttgtccacc gaacagcaga agtacatcga caattccgaa cccggcgagg gccttttgat
     8641 ctttgaaaac gtggtgctgc cctttaccaa ccccatcccg cacaacaccc agctttacaa
     8701 gattatgacg acccgcttaa acgaggtggc gggagtatga cccggcgacg ccacaaagga
     8761 ggtgaaccac catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc atgacccgcg
     8821 acgggctgac gctggaaaat caggccaccg gcgaggtgga gaacatttcc agccgggagg
     8881 ccgaacagga tttgtccgcg ccggggccgg gcggcaccgc cgataagctg ctggaacggg
     8941 ccgacgccat ccatgacggc cataaggcca agaaagccgc gaagcaggcc ggggctgtgg
     9001 ccgccgaggg ggacgctgcc ctgcaccggc catccgcccg gctccaactg tcggaggaag
     9061 aacgggccga cccggattta cagccctata tccgaaaatc ggacaaacgg gccgataagc
     9121 tggataaggc ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa accgtctttg
     9181 acgaggcaag cggcaaagca aagaccgtcc tgcactttga caaacaggac aaggggcctc
     9241 cctccctgaa acccaatccg gcaggccgtc ctgtttccga ggtttttcta ttcggccacg
     9301 ggaaaatcca tgaagtcgag catgaaaacg tgggtgtgga gggcggccat aagggggagg
     9361 aacttgccga gcggcaggcc ggaaaagcca tccgcagcac catccggcac cacaggctga
     9421 aaccctactg ggacgcggaa aaggccgagc gcaggctatt gtctgccaac gcggagtatt
     9481 tctaccagaa atctttgcgg gataacccgg agatcgcaca ggcggccagc aaccccattt
     9541 cccggttctg gcagaaacag cagatcaagc gccagtacgc caaggccgcc cggacagcgg
     9601 gccagaccgc aggcgcggcg gcacagggcg cggccaagac agccggggcc tccacgaaag
     9661 cggccaaggc cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc cgccactgga
     9721 aaggggcgct ggccgtcctt gtgttggggc ttttgatgct gcttatcatc ggcggcttac
     9781 agtcctgcac cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc tcttactttt
     9841 ccgaggatag cgatatgttg gccgccgagg acgcctacgc ggagctggaa gccgagctgc
     9901 aagactatct ggacaactac gaggccaccc atgactatga cgaataccat tttgaccttg
     9961 acgagatcag ccatgacccc tatgtgctga tttccattct gtcggcgctc catgacggga
    10021 tatttaccat cggggaggta cagggtgatt tgtccatgct ctttgacaag caatatatcc
    10081 tgacagaaaa cgtcgtggta gaaacccgtt accgcaccga aaccgataca tggacggacg
    10141 cggagggcaa cacccataca gacacctacc aagtgccgta tgactattac atctgctacg
    10201 tcacgctgga aaacttcgac ctgtcccatg tgccagtcta tatcatggac gaggaaacgc
    10261 taagtttcta ttccctgtat atgtccacgc taggaaaccg gcctgatctg ttttccggca
    10321 gccagtaccc caacgcctcc accctgacgc cgccgaccta ctacgacgta cccccggagg
    10381 ctttggagga tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat atcggttatc
    10441 cctatgtgtg gggcggcagc tctcccagca ccagctttga ttgcagcggc ttcatttcgt
    10501 gggtaatcaa tcatagtgga tggaacgtgg gacggctggg ggcgcagggc ctatataaca
    10561 tctgcacccc tgtttccagt tcgcaggcca agcccggcga ccttgtattt ttcaagggaa
    10621 cctacgacac ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat gtgatgctgc
    10681 attgcgggaa cccgatttcc tacacgaacc ttacttcaaa ctactggcaa tcccattttt
    10741 acagttacgg gcgtttacct taacgccaga aaggagcttt caattatggc aaccaaactt
    10801 gaccgcatcg aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt tcagaaacag
    10861 cttcgggagc tggaaacgca gaaaaccgaa caggagaatt tgcagatcat ccagcttgtg
    10921 cgcggcatga acatgaagcc ggaggaattt gccgcgttct tacgcagcgg cgcaatgcag
    10981 gccgcccctg ccgcaacccc ttaccatgaa caggaggaca atgcccatga agaataagac
    11041 cagacggatt tcccgtattc tccccgcact tctggccgtg atgctctgcg tcacggcctt
    11101 tcccgttacc gcccttgcag gcggcaatga cccggcaccg gccccgctgc cggaggctac
    11161 aaccgaaccc gctacgggcg gtttggagcc tgaaacagac gaaaccggca ccgccacgga
    11221 cgggacagaa accgaggccg aaaaagatac cggccctctg accggcaagg acatcaccga
    11281 cctgttttcc gcgctgttcg gttccaaggt gagcattgcc gccacggacg acggcatcca
    11341 gatcaccacc ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca ccaccaacgg
    11401 cggcaggctg aatgtccgca ccggcgcggg gcttgacaag accgcgttca cccagcttcc
    11461 caatgggacg acggttgagg tagtcggcac agacggcgac tggattaaaa ttctcttgcc
    11521 ggagcgcatc ggctatgtcc attccgacta tatgaccgtc agcgaaaagg aagtagcggc
    11581 taccggcgag ggcggttttt ccctctccat tgacccggag gaaattgctt ctctgctgga
    11641 actgttcaat ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc tgtcactgat
    11701 tgacgacatc ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg aaaccaaaaa
    11761 cggcaatgtg ttttatctga ttattgaccg ggacgacgag ggaaaagaaa ctgtccattt
    11821 cttaaatcag gtggatgaag ccgatctgct gacgctgatg gaggacgggg aaaccgtcac
    11881 ccccgctgct gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga acacgaattg
    11941 cccggtatgt aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg aaccccagcc
    12001 ggaggaaccc gaagccccgg aggaagaacc caagggcggc gcgggcggcc tgatcgtgtt
    12061 cctgctggtg gcgctggcgg gcggcggtgc ggccctttac tatttcaagt tcaaaaagcc
    12121 caaggctgaa accaccggca gcgacgattt gggcgagtat gattttgggg aggacgagga
    12181 tttggaggac gaggaacccg aaaccgagtt tgacccggaa gctgattttg tgagtgaaca
    12241 ggaggacgag gaatgacata taaacttgta atcgcggaaa aacccagcgt ggcccgcagc
    12301 attgcgggcg tgatcggggc cacggagaaa cacgacggct atttggaggg gaacggctat
    12361 ctggtgagct ggtgcatcgg acatctggtg tcctttgccg acgccggacg ctatgacgag
    12421 cgtttcaaaa aatggcgcta tgaggatttg cccattctcc cggaaacatg gcagtacatc
    12481 gtccccgacg acaagaaaca gcagtttgac gttctctgtt cgctgatgca gcgccccgac
    12541 gtgaccggcc ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat tttccgtttc
    12601 gtctaccaga tggcgggatg ccaaaagccc tttgagcgcc tttggatttc ctctatggag
    12661 gacagtgcta ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta tgatccgctg
    12721 tatcaatcgg ccctgtgccg ggccaaggcc gactggctga tcggcattaa cgccacccgg
    12781 cttttttccg tcctgtacca caagaacctg actgtgggcc gcgtccagac gccgacctta
    12841 aaaatgctgg tggacaggga cgccaagatt accgatttca agaaagagaa atatcacatc
    12901 gtccatatca cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga cgctgccgag
    12961 gcaaacaccg tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc cgtgacgcgg
    13021 gagaaaaaga cggaacagcc gcccaagctc tatgacctta ccaccttgca gcgggaggct
    13081 aaccggcttt tcggctatac cgccaagcag acccttgact atgcccaggc actgtatcta
    13141 aagaagctgc tgacctatcc gagaacggat agtcaatatc ttaccgacga tatgctgccc
    13201 gccgtggaaa gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa gggcctgaac
    13261 ctttccccgc agtttgaccg catcctgaac agcaaaaagg tgtccgacca ccacgccatt
    13321 atccccaccg ctgaatttgt gaagcagggc tttggcactc tggccgagag tgaaaaaaag
    13381 ctgctgtccc tgatctgctg caagctctta tgtgccgtgg ccgggccgca tatctatgag
    13441 gccgtcaccg ccacatttac ctgtgccggg caggagttta ccgccaaggg caagaccgtc
    13501 ctttcccccg gctggaaaga gattgaacgc cggttccgtt cttccttaaa gaccgacgcc
    13561 gacgaggacg ccgaggcggt gcgggagctg ccggagcttt gcgagggcca gacctttgca
    13621 gacgtggccg cctctgtcac cgagcatttc accacgccgc ccaagcccta caccgaagat
    13681 acgcttttgt ccgctatgga acgggccggg gccgaggata tgccggagga tgccgagcgc
    13741 aagggattag gcacaccggc cacccgcgcc gccattctgg aaaagctggt gcagatgggc
    13801 tttgtccaga gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa tctggctgtg
    13861 gtgctgccgg aggcgcttac ttctccccag cttactgccg aatgggaaaa ccggctgacc
    13921 gagatcgcca aggggcaggc cgatcctgac gaatttatgg acggcatcga gacgcaggcg
    13981 cgggagctgg tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt gtttcaggcc
    14041 gagcgtgttc ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga gggcaagaaa
    14101 aactactact gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga ccgatttttt
    14161 gaggaacgca agaaagcgtt caccccgaag atcgccgctg cgctgctcaa aaccggcaaa
    14221 gtcaaggtaa agggcctcta ttccgtaaag accggcaaaa cctacgacgg gacggtgctt
    14281 ttggctgata ccgggggcaa gtacgtcaac taccgcattg aacacaggag ctaaaggaca
    14341 gtaacacagc aagcataggg agtgtggcta cactcccggt aaaatccccc ggccgcgtcc
    14401 ctgccgggcc ggggattttt gcttgttggg gaagtcccca accccctatt ttttgaaaca
    14461 gaacagagaa aggagtgacc gcgtgaacga agtattttct atccaagtcc ataaccggca
    14521 gtttttggaa cagggcaaac aaggcgtctg gctggatttg cccaccacaa ccgaaacgct
    14581 gcaagccgcc ctgcgggatg ttggcattac tgccgacaat ccgcaggatt tttttatcaa
    14641 cgggtattcc tgcccggagg acagacacct tgccattccc tatgacatgg tgctggccgc
    14701 cgacgtggac gagctgaatt tccttgccgc ccggctgggc cagcttgacg ccgccgagat
    14761 cgcagagctg aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata tcggccagat
    14821 catcgactac accgaaaatg tggattacta tgtgcatctt cccgacgtta ggggggatgg
    14881 acagttgggc gactactacc tgaaccgttc cggcatggtg gatatgccgg aggaatggaa
    14941 agccgggatt gatacggcgc agtttggacg ccacattgcc cagcaggaac aaggcgtttt
    15001 tacccagtac ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc atgagggcca
    15061 gccggtgccg gaggaatacc gggtgatgca ctacccccag ccggaagctg accgggagga
    15121 accgcaggcc cagcagccgg aggcatccga aagccccgac aaagcgccgc ccccggttat
    15181 cccgatcatg ctggacggca tgaacagcaa agaacgcatg aaagtgatta ctgaccgtct
    15241 ggaaaccggc gtacaggagc tttttgaaag tagccgctat atggcttact tatccgctat
    15301 gtccaagatg cacgattaca gtctgaacaa tacgctgctg atcgccatgc agggcggttc
    15361 gattgtggcg ggctataaca ggtggcagga tgaatttgac cgccatgtga agcggggcga
    15421 gaaaggcatt aagatactgg ccccggcccc gttcaaggtt aagcagcaga tggagaaaat
    15481 cgacccggcc acccagcagc ccgtgatcgg cgcggacggc aagcccgtga ccgaagaaca
    15541 ggaagtgacg gttccggcct ttaaggtggt gacagtcttt gacatcagcc agaccgaggg
    15601 caaggatttt cccatgccct atatcagtga acttaccggc gacgtgcagc agtatcagga
    15661 tttctttgcc gcgctggaac ggacatcacc ctatcccata agcattgagc cgatttcaga
    15721 cggggccaag ggccgctgct tctacgcaaa acagcgcatt gccatcaacg agggcatgag
    15781 cgaattgcag accctaaaga ccgccatcca tgagattgcc catgccaagc tccacgccat
    15841 tgaccgggag gcccccgccg aggaacaggc ggcccggcca gaccgcagca cccgcgaggt
    15901 acaggccgaa agcgtggcct ataccgtctg ccagtattac gggctggaca cgtcggacta
    15961 ttcttttggc tatgtggccg attggagcag cggcagggag ttatccgagc tgaaagcctc
    16021 ccttgaaacc atccgggcca cggccagcga attgataacc gagatcgacg gccattttgc
    16081 ccagcttcag caggagcgcg aggcccggca ggaacaggag gccgtcacgg ttgacgttac
    16141 cccggaggca gcggccccgg cgcaggccga ggaagaacag gccccggccc ttgatccggc
    16201 agccgagccg cttgtgactg tgctatggag cgaaagcccc cagctaaagg acggccagca
    16261 aatgccgttg catgaggccg acgcgctgtt tgcccgtctg gatgcagcat atccggcaga
    16321 ggcaggctat gacaaaaccg cgttccgcat tgactgtatc tttcagggcg agccggacag
    16381 ctacgagggg cggcaggatt ttggagatag ggacggttcc ctgatcgaac atatccagag
    16441 ctaccaccaa ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg ctacaaccgg
    16501 catagagggc tgggagcagg acaaggccca tcgggatatg ctgttaggcg agtttgtccc
    16561 ctatatgaag ctgcattgca gccttgccca aatggaacag acggcgcagg gccgcttaca
    16621 gtccggggaa accctgacgc cggaggaaac ggcctatttg ggggcgcttc tctcttatgt
    16681 gcaggagtgc cgccccatgc tcaatcaggg ccagtatcag ttgccggagc cgccgaaact
    16741 ggctgacttc gaccagagct tgcaggacta caaagcacag gtacaggccg agatcgcgca
    16801 ggaggccgcc gcagccggta tgaccgtgga ggaatacgcg gcggctggct atgaagcacc
    16861 ccaacaggac accttttcca tctaccagct tcgaggcggg gaggaaaccc gcgactatcg
    16921 gtttgagcca tacgagcgtt tacaggccgc agggctggcc gtggacagtg ccaactatga
    16981 ccttgtttat acggcaccgc tggataaaag cacgtctttg gaggatattt accgcacatt
    17041 caacatcgac cacccggcag attttagggg acacagcctt tccgtttccg acgtggtggt
    17101 gctgcaccag aatggacagg acaccgccca ctatgtagac agcttcggct atatggaagt
    17161 gccggagttt ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg agcagaacga
    17221 aaacatgatt gacggcatta tcaacaatac gccgcccgaa ccgcagccgg aggaacccga
    17281 acagctatct tttgaggatg tggcagttgc ttccggccag acacaggagg aacccccgga
    17341 ggctgcgccc ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag ccgcccgccg
    17401 cgccaaggaa gccatgagct tttctgacta ccggcccggc agcgccacgg cggaataccg
    17461 gcactatgtc gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg tggagccaga
    17521 gtatcacgaa aagatagaca gcctccttga cacctacgcc cgcaagctgg ccgagaacat
    17581 gaataagggc tatgagatta ccgcccgcgt cccctctgtc ctgattgccg ggccgtccaa
    17641 tttcccggta cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca tggaggaatt
    17701 tcagtacatt cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg gtatcagcgc
    17761 cgacgatccg caggccgttt ccaagctgga aaagaaactg gaaaagctgg aagcgtcgca
    17821 ggagttgatg aaggccgtca atgcctacta ccgcaagcat gggacgctgg acggatgccc
    17881 ccatctgacc gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg gctggcacta
    17941 tgagaaaaaa ccgttccagt cctggcagct ccccaacaat aacgccgaga tacgccggtt
    18001 aaagggccgc attgaggaat tgacccgcca aaaggaggcg gcctatgtgg gatgggagtt
    18061 tgacggcggc actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct tctttgaggg
    18121 gaagccggat gctgctgtac gcgacgagct gaaaagcaac ggtttccgct ggtcgcccaa
    18181 ggccggggca tggcagcggc agttaaacga caccacaatc cgcgtcgctg accgtatcaa
    18241 gtgcatccag cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg cccgcaggga
    18301 gaaagaaaag ccctctatcc gggcgcagct caacgcggcc aagaccgaac aggccaagca
    18361 gccaccggcg cgggaaaaat caaagggatt ggaggtagag taaatgcaga agtttgaaaa
    18421 tgtggacgtt ctggccgcgc tggaacagat catgcggcag aacaccgcgt tttatcaaag
    18481 tgactttggc attgacaaag acattatcag gcgggatgcc gccagcgatc aggccgcaga
    18541 caagaccctg ctctggatgt cccggccctc cgggacgtac tgtttccggg aacgtgatgt
    18601 tttcctgaaa gacacccgac agtacaacac atggaaattt tacggggagc agacccgtga
    18661 caagatactg gcctacgcgg tggagctgac cggcacacag ggcggcacta tccggggcaa
    18721 tctctatgag ctggactatc cgcagcattt ccggcacgtc attgaaaccg cccagccggt
    18781 gagtgtgaac cggctctttt atgagcatgg cacccgtgac atcccggaga gccagcgttt
    18841 tgacggaagc cctgaccggg tattgggaaa ctttttgtac tatgaggccc agccccatga
    18901 cccggctgtg ctgcaggagg cgctacggac ggagcagcac agccgggggc gggccgtccc
    18961 cggcgatttc aaagtccacg tcaccgcttt gcatgacagc cggattgaaa ccgaggcccg
    19021 gcgtattgtg gacagcctga aagatctgtc cgcgcctaac agccccaata aaacgcattt
    19081 catggtggag atctccccgc actttgcggc gattgcttcc accaaggata atgaccggct
    19141 ctttgccatg ctgccctata aatccctgtg ttttaccggg atgaaaggca ggcatggcgt
    19201 gtatgccgtg attaaccggg acgagcgccg ggatgtgagt attcgcaggc cccggccctc
    19261 tatccgcaag cagctatccg acagcaaaca ggccgccagc ccaaaaaagg cggcggccag
    19321 aaccaaaaag aatgaattgg aggtataagg aatgatgcac tttaccgtgg aggaagaaaa
    19381 cctgatctgt atgtaccaca atgcagaccg gcgcagaacg ataaacaggc tcaccgccgt
    19441 catgccggtt atggatgggg atatgcgggc gcaggcccag cagacccttt ccaagctgga
    19501 acgcatgacg gatgccgact atgacggcca gaagttccac ttcacagacg aataacgaac
    19561 agcagacgcc gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt tctttttgcc
    19621 tattcctacg catttagaac ccggtattgt cggggatagg atgaagtatt cgcttgcccc
    19681 tgcaaacatg cgggagaagc cttgcagcgg gcggttttca atcccttaaa tgcgtaggtt
    19741 gcagctataa ttagacccct gaaacagata tagccgcgca gcgggtgtat ctgtttcagg
    19801 ggtgtgcagg tgatagccgc aaagcggcgt aagagggtgc agccctcttg ccgtctatct
    19861 ctgttccagc gggaagctcg gcaatgcttc cagcagtttc agggaaatgc gctgccgtat
    19921 atcttcgtca atgactttct tctttttgcc gtttgcctgt ctgatctcca ccgtggaaag
    19981 ctcgttgatg tagtcagcat aatgggccaa cactttttcc gtggcccacc tctcaccggc
    20041 aacggcggct ttgatcgttt catagtccag cagcttttct tgtccgtcca tcctgatacc
    20101 cccgtcagac tgattttttc tatgatacca tattcggaag catggaacca gagaaaacgg
    20161 cgcttttctg ctggtaaatt ctacgcattt agaacagcac ttcggcgggg ataggataaa
    20221 acacccacat accccgctga ccatgccgga aaagccttga aatataaggg cttttacagc
    20281 tctaagtgcg taggtttcca gtatcttttc cgctggcgct cggcctcttt gcggcggcgc
    20341 atccgtttgg agcaggccgc gcagtatttg gcccggttag acttcggcgt gaacgtggcc
    20401 ccgcactcac agcagcgttt cacttcgtcc cggtctttgg tgatctcggc atacagggcc
    20461 gcatccagcg ggaggacagc ggccttaaac cagcggcaca gaagcgaata ggaaatgctc
    20521 tgtacgcaaa tacattcttc gccgtcgtcc agcaacaggc agttgccgtc acaatagttg
    20581 cagcactcat gcgtcagctt ccgggccttg cgatattgag ggtaattcat tcgtggaata
    20641 ctcatagctc ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg tccacattct
    20701 gcttgatgat gccgtattcc tgcatctgct ttttcgcttc gccgtacttc tcatacagcc
    20761 gttccttttc ctcggccaat cgcttgtact cggctttcag ggcgtacaga tcggggagct
    20821 ttttcacacc ctgtcccttt agggcctttg ccgcagcttc atacaaaatc aacgtgcttt
    20881 catgctcacg ccggaatttt gccttatccg ctgcattgcg gtattccata gccacgggcc
    20941 agagctgctt gtaggtagaa atattcttta tcagcaccgc catatccgca agccggtgtt
    21001 ccatagcttt gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg gccactttgc
    21061 tttccaaatc cgggtaggtg tctatcccat gttcagtcag gaaattcaaa gtctttgcgg
    21121 cctgcttcaa attgtggagc ttcgcccacc tctcatagcc tgccgactgc tgggccttaa
    21181 tgttgttttc cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc gctctggtga
    21241 taacgcggcc tttgatacgc tctttgatgg cttcctcggt gtaggcttcc cccaatgtct
    21301 tgcagcgggt aaagcgttcc tgtccgggag cgcggaaaga aataaacttt cccggcttga
    21361 tctcatagcc ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt gcctgcggga
    21421 tagcggcgtc aatggctgct ttgagcttcg ctttccagct cgtccctttc cgctgggcgt
    21481 cccactcggc atagctcttg cccttactct ggccgggctt caccacggac aatccgtttt
    21541 ccttgcacag cctgtcgctg gtgcgccgga tataggcata gctctgtttg ttggaaatgt
    21601 acttgcgctg atttaccata tccacggcac agaaaataat gtgattgtgg acgtggccct
    21661 tatcaatatg ggtagtcagc acatagggat atttcccttg cagcacttcc tcggcaagct
    21721 gcttcccgat ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag gattgtatca
    21781 ggtggtggcc cagattattg cccttttggt atgcctgcgc cagcagcatt tcaaattcaa
    21841 tgtccgctgt ttcataagag caggcaaagg aggccaccag cagcttgccg tcagttttgt
    21901 ccgggttctc gatgtagtca agggcttttt tcagcgttga cttgatagga tgtatctttg
    21961 taactgccat atctgcgcca gcgccccctt tatatcctcg atgtcctgtg cgtacaccgg
    22021 ccccataccg ttcagcctct ttgcaatctg attgacgttg acgccgatct tctgtatctc
    22081 ggcggtctgc gcccggatgt cgctggtgtc catgttgacg atatagccgt caatgagcat
    22141 cttccgggcg taggctgaaa agttcttcgt atgcagcagc gccattttct gttcaatcat
    22201 agcccgttcc tccggcgtca cagggacgcg cagcacgatt tttcgctttc tgtttgccat
    22261 gcttcaccgt cctttccatt caggggtttg gggatttccc caacaagcaa aacggacgcg
    22321 gcccggcagg gacggggcag gccgtttttc cgggagtggc tacactccct atgcttgcta
    22381 cgttactgtt tctccactaa caagacgttt ccaaagcccc aaactacccg caaaatagcc
    22441 ctctggaaag aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt
    22501 aaaacagagg atgccagtta cacatcctcc gtttcattct tcttcggctt ttcaattagt
    22561 atggcgctgt caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca gagtttcagc
    22621 ttgtggctga ccctttgccg cagttcgctt tcctcccgca tgatctccgg gttaaagtag
    22681 cgttccacag gaagcccgca ggctttaata agctgtatga caaccggcag actgggaatg
    22741 gccccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt ctctgccatt
    22801 gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc gaaagtttca
    22861 aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt gtatatttca
    22921 tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt tcacattttg
    22981 tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt actatggact
    23041 acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg ataaactact
    23101 attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg ctcattccca
    23161 aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt actgaatagt
    23221 ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat ggtattttgt
    23281 cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa aagtattctc
    23341 atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa ctttatcatg
    23401 acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc agccgtatat
    23461 ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag acattggata
    23521 actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg gggaggtacg
    23581 gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt cctgtaactg
    23641 caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct actgtcttga
    23701 acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga gggagtacac
    23761 acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg
    23821 cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa
    23881 gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag tattatcggg
    23941 atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt taattattat
    24001 tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga aaagaagcga
    24061 gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact acagggaaac
    24121 tacagactgt tatgttatct tcttaaaatg gagggatttt tatgtcgata cgaattctac
    24181 ttgtcgagga tgatgatcat atctgtaata cagtaagggc gtttctggct gaggcaggat
    24241 atcaggtgga tgcctgcaca gatggaaatg aggcatacac caagttttac gaaaacactt
    24301 atcaactggt tattcttgat attatgctgc ccggtatgaa cgggcatgaa cttttgcgtg
    24361 aatttcgtgc gaaaaatgat actcccattc tgatgatgac agccctgtcg gatgacgaaa
    24421 accaaatccg ggcgtttgat gcagaggcag acgactatgt aacaaagcca ttcaagatgc
    24481 agattttact aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg gcaaaggaaa
    24541 tccgtgtcgg caggctgaca cttctgccgg aggatttcac ggtactttgt gacggtacag
    24601 agctgcccct gacacgaaaa gaatttgaaa tccttttgct gctggtgcag aacaaaggca
    24661 gaaccttaac gcatgaaatc attttgtcac gtatatgggg atatgacttt gagggtgatg
    24721 gcagcacagt ccacactccc acactcatat caaaaatttg cgggccaagc tgccggaaaa
    24781 tatcatcaaa accattcgcg gtgtaggata ccgattggag gaatcataat aatggaaaga
    24841 aaagggattt tcattaaggt tttttcctat acgatcattg tcctgctact gcttgtcggt
    24901 gtaacagcaa cactgtttgc acagcaattt gtgtcttatt tcagagtgat ggaattacag
    24961 caaacagtaa aatcctatca gccattggtg gaactgattc agaatagcga taggcttgat
    25021 attcaagagg tggcagggct gtttcactac aataaccaat cctttgagtt ttatattgaa
    25081 gataaagagg gaagcgtact ctatgccaca ccaaatgcca atacatcaaa tagttttaga
    25141 cccgactttc tttatgtggt acatagagat gataatattt cgattgttgc tcaaagcaag
    25201 gcaggtgtgg gattgcttta tcaagggctg acaattcggg gaattgttat gattgcgata
    25261 atggttgtat tcagcctttt atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc
    25321 aaagccttag cggacagtgc gaataaaatg gcaaacctga aagatgtacc gccgccgctg
    25381 gagcgaaagg atgagcttgg cgcactggct catgatatgc attccatgta tgtcaggctg
    25441 aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca
    25501 cagcgatatt tctttgcggc agcttctcat gagctaaaaa cgcccatcgc ggctacaagc
    25561 gttctgttgg agggaatgct tgaaaatatc ggtgactaca aagatcattc taagtatctg
    25621 cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca tttccgaaat actggagctt
    25681 gtcagcctga atgatgggag aatcgtaccc atagctgaac cgttggacat agggcgcacg
    25741 gttgccgagt tgctgcccga ttttcaaacc ttggcagagg caaacaacca gcggttcgtc
    25801 acagatattc cagccgggca aattgtcctg tccgatccga ggctgctcca aaaggcacta
    25861 tccaatgtca tattgaatgc agttcagaac acgccgcagg gaggcgaggt acggatatgg
    25921 agtgagcctg gtgctgaaaa atgccgcctt tttgttttga acatgggcgt tcacattgat
    25981 gatactgcgc ttccaaggct gttcacccca ttctatcgca ttgatcaggc gcgaagcaga
    26041 aaaagtgggc gaagcggttt aggacttgcc atcgtacaaa aaacgctgga tgccatgagc
    26101 ctccagtatg cgctggaaaa cacctcggat ggcgttttgt tctggctgga tttaccgctc
    26161 acatcaacat tgtaaatatt taaaacttaa atgattttga ccgacaggta taaccctgcc
    26221 ggtctttttt tgctgctaca ggaaaactac agactggcta cagggaaagt acagatgcgc
    26281 ttgccataat aacaatcgta ccagccacaa atcgtagttt tattgcaaag gaggcattcg
    26341 atcaaatgga aaaaagcaac tatcattcca atgcggatca tcacaaacgg catatgaaac
    26401 aatccgtgga aaagcgggct tttctatgtg cgttcattat ttcgttcaca gtctgcacgc
    26461 tgtttttggg gtggagactg gcttccgtat tggaggcaac acagataccg cccatccctg
    26521 caactcatac aggcagcagc actgacgtag tggagaattt ggaggaaaac gctcttgcca
    26581 ccgccaaaga acagggagat gaacaggaat ggagcctgat tttagtgaac aggcagaacc
    26641 ccatccccgc acagtacgat gtggagcttg agcaactatc aaatggtgag cggatagata
    26701 ttcggatttc tccctatctt caagatttgt ttgatgccgc aagaactgat ggagtttacc
    26761 cgattgtcgc atccggatac cgaacaacag aaaaacagca agaaattatg gatgaaaaaa
    26821 ttgccgaata taaggcgaaa ggctacacct ctgcacaggc taaagcggaa gcagaaactt
    26881 gggtggccgt gccgggaacg agcgagcatc agcttggtct tgctgtggat atcaatgcgg
    26941 acggaattca ttcaacaggc aacgaggttt atagatggct ggatgaaaac agctatcgtt
    27001 ttggttttat tcgtcgctac ccaccagaca agacagagat aaccggtgtg agcaacgagc
    27061 cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga gatgtacaac caaggggttt
    27121 gccttgagga atatttaaaa ccagaaaaat gagaaaagga tgttatgtta tggacagaaa
    27181 aagattgaca cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg
    27241 cttttatgcg ggaatgagat ttgacggctg tcgctatgcg cagacgatag gggaaaaatc
    27301 gctttcccat ttgctctttg aaacggattg cgcattatac aaccacaata ccggatttga
    27361 catgatatac caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg
    27421 cttattgata agaccggggg aaaccttttc tttctggtgg ctggtacgcc atgcggacaa
    27481 agataccccc tataaagacg gccttacggt gaccaatggt aaactcacca ccatgtcggg
    27541 cggcggtatg tgccagatga gcaatttact attttggatg ttcctgcata cgccattgac
    27601 aattatccag cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca gtgacgagat
    27661 taaaggggtg gatgcaacca tttcagaggg ctggattgat ttaaaagtgc gaaacgatac
    27721 cgactgcacc taccaaatat gggtgacctt agatgatgag aaaatcatcg gtcaggtgtc
    27781 cgccgacaaa gagccccaag cattatacaa aattacaaat ggcagtatcc agtatgtccg
    27841 tgaaagtggc gggatttatg aatatgccca ggttaaacgg atgcaagttg ccttaggtac
    27901 cggggaaata atagattgca agctgcttta tacaaacaaa tgcaaaatct gttatcccct
    27961 cccggaaagt gtggatattc aggaggagaa ccaatgagaa aaagtgtggg cattactgtt
    28021 tttggatgcg aacaggatga ggcaaatgct ttccgcgctt tatcgccgga ttttcatatt
    28081 atccctacgc tgattagcga tgcgatatcg gcagacaacg caaaattggc cgctggcaat
    28141 caatgcgtta gcgtaggcca taagtccgag gtttccgagg cgacaattct tgcgctgaga
    28201 aaggtcgggg taaaatacat ttctacccgc agcatcggct gcgatcacat tgatacgact
    28261 gccgccgaga gaatgggaat ctcggttggc acggttgcgt attcgccgga cagcgttgcg
    28321 gattatgctt tgatgctgat gctgatggcc atacggggtg caaaacccac catgcacgcc
    28381 gtggcgcaac aagatttcag attggatcgt atccggggga aagaactggg ggatatgact
    28441 gtgggagtta ttggaaccgg ccatatcggg caagcggtcg tcaaaaggct gcggggattt
    28501 ggatgccatg tgctggccta tgataacagc cgaaaaatgg atgcagatta tgtccagctt
    28561 gatgagcttc taaaaaacag cgatattgtt acgctccatg tgccgctttg tgcggatacc
    28621 cgccatctga tcggtcagaa gcaaattgga gagatgaagc aaggcgcatt tttaatcaac
    28681 accgggcgcg gagcgcttgt cgataccggg gcgctggtgg aggcgttgga aagcggaaag
    28741 ctgggtggtg cggcactgga tgtgttggag ggcgaggatc agtttgttta taccgattgc
    28801 tcgcagaaag tgcttgacca tccctttttg tcgcagcttt taaggatgcc gaatgtgatc
    28861 atcacacccc atacggcgta ctacactgag cgtgtgctgc aggatactac agaaaaaaca
    28921 atcaggaatt gtctcaattt tgaaaggagt ttacagcatg aatagaataa aagtcgcaat
    28981 catcttcggc ggttgctcgg aggaacatga tgtgtcggta aaatccgcaa tagaaattgc
    29041 tgcgaacatt gatacggaaa aattcgatcc gcactacatc ggaattacaa aaaacggtgt
    29101 atggaagcta tgcaagaagc catgtacgga atgggaagcc gacagtctcc ccgccatact
    29161 ctccccggat aggaaaacgc atgggctgct tgtcatgaaa gaaagcgaat acgaaacacg
    29221 gcgtattgat gtggctttcc cggttttgca tggcaaatgc ggggaggatg gtgcgataca
    29281 ggggctgttt gtattgtctg gtatccccta tgtgggctgt gatattcaaa gctccgcagc
    29341 ttgcatggac aaatcactgg cctacattct tacaaaaaat gcgggcatcg ccgttcccga
    29401 atttcaaatg attgataaag gtgacaagcc ggaggcgggt gcgcttacct accctgtctt
    29461 tgtgaagccg gcacggtcag gttcgtcctt tggcgtaacc aaagtaaacg gtacggaaga
    29521 acttaacgct gcgatagaag cggcaggaca atatgatgga aaaatcttaa ttgagcaagc
    29581 gatttcgggc tgtgaggtcg ggtgtgcggt catggggaac gaggatgatt tgattgtcgg
    29641 cgaagtggat caaatccggc tgagccacgg tatcttccgc atccatcagg aaaacgagcc
    29701 ggaaaaaggc tcagaaaatg cgatgattac agttcccgca gacattccgg tcgaggaacg
    29761 aaatcgggtg caggaaacgg caaagaaagt atatcgggtg cttggatgca gagggcttgc
    29821 ccgtgttgat ctttttttgc aggaggatgg cggcatcgtt ctaaatgagg tcaataccct
    29881 gcccggtttt acatcgtaca gccgctaccc acgtatgatg gccgccgcag gaatcacgct
    29941 tcctgcactg attgacagcc tgattacatt ggcgttaaag aggtgataag tatggaaaat
    30001 ggttttttgt ttttagatga aatgttgcat ggcgtccgtt gggatgccaa gtacgccaca
    30061 tgggataact tcacaggaaa accggtggat gggtatgagg tgaatcgcat catcggcaca
    30121 aaggccgtgg cgtttgctct gcgcgaggca caaatccatg cggctgcgct tggctatggc
    30181 ttgcttttat gggatggata tcggccaaga actgcggtgg actgcttcct gcgttgggca
    30241 gcgcaaccgg aggacaatct cacaaaagaa aaattttacc ccaatataga gcgagccgag
    30301 ttgattacaa agggttatgt ggcctcacaa tccagccata gccgtggaag cgcaattgat
    30361 cttacgctct accacctgga tacaggggaa cttgtttcaa tgggaagtaa cttcgatttt
    30421 atggacgaac ggtcgcatca tacagcaaaa gggatagggg atgcagaggc acaaaatcga
    30481 agatgcttgc gtaaaatcat ggaaagcagc ggatttcagt cttatcgctt tgaatggtgg
    30541 cactataagt tgattgatga gccatacccc gatacctatt ttaattttgc cgtttcataa
    30601 tgaaagtatt ggttttgtat cagattttgg atttgataaa atctatgctg gtcatggaca
    30661 atcttttact ggaaagtggt taaagcatct tttgtaatac attgcaaatt atagtattca
    30721 atctgcccag cggcagaaaa gaaaaaaacc gctgttgggc agacctttcc cacgcccatt
    30781 ttgtattttg ctggcggcgg ttttgagaaa tgaaccgccc cctgtcaagt agacagatga
    30841 aaaaagaaaa attacgccac gagggtctgg ttcctgtaag caaggggagc caggcccttt
    30901 aacttagctt gaagccgcct tgtattgtaa aagtctatgt attcgtcgat ggctttcttc
    30961 aattcctcga aggtatgaaa ttgccggagg tagtacattt cagatttgag aatcccccag
    31021 aagccctcca tagggccgtt gtcgatgcag cggccgggcc gggacatact ctgcgttgca
    31081 tggataccgt ccagctttgc tttgaactgc cggttggtgt actgatagcc cctgtcgctg
    31141 tgaaacagag gatgggcgcc ggggttggct ctcaccgctc tgtcaaaggt gtcaaaaaca
    31201 agctgattgt tgttggattg gcccagtgca tacgcaacga tgcttttgtc atggagatca
    31261 aggatcgcac tcaggtatgc tttcttccca ttgataagtt tgaattctgt gacatctgtc
    31321 agccatttct cattgggacc atttgccgta aattctctgt tcaatacgtt ttccgcggta
    31381 acacgcggtt ctaatatgac atagtgcttt ttctttcggc gtatcacggc ctgcatatgt
    31441 acacttttca tcaggcggta aatccgcttg tggttgtagt ttcttttgag ccggctgttc
    31501 acattcagcg tcatacggcg ataaccgtag atgcctttta cttcgctgta caaacggatg
    31561 atttcttcca gcaagagggt attttctttt tctgaggcac tttcttcccg gtgaagccat
    31621 ttgtagtaag aacagcgggg aatcccggat aacgcgcata acgatgcgat ttcatatcct
    31681 ttttcctgac gaagcgtatg gatcgcctca tagaccttcc tgcggcgtgt gagcctgttt
    31741 accgcctccc ctccagttcg ttcaattttt ttaagaaatc gttctccatt tgaagccttc
    31801 tgttttccgc ttccagctgc cgcagctttg ccgcggcttt ttcctcctcg ctgtattcgg
    31861 aaggagggcg gcgtctcccc cggcggtcaa gcaacccagc ttctccctgc tcctgatatt
    31921 ttttgaccca gctatagatc tgctgatagg tgatcttgta ctgttcgctg gtccgccggt
    31981 agtccagttg atgttctaag cagtatagta ctgcctgcag cttttgctcc tgcgataatt
    32041 tttctttctc ggccatatct gtttcctccc gcgctctgcg tgtcttgaac tctctatggc
    32101 cttgattata caacgtaatc cattgttgta aaaccgcatg ggaggaaatc ccgtatttgc
    32161 ggcacatggc atccagcgag cctcctccgc ccatgtagtc ttctactgcc gccagcttca
    32221 gtgaggatgg ataacttgta acatgctttt tcggatgcag acccgcaggc ccctccgtct
    32281 gatatttccg cagccatgcc tggaaagtgc tcaatcgtat gcctaactct tcccggatct
    32341 gtgtggaacc cttacggatc gctaggtagt cctccacagc ctgaatcttt acctccggcg
    32401 gtattttgcc ttttaaactc atgataattg ctccctccgt ttcgacagtt taattttttt
    32461 gctgtctact tcagagggag catatcaaaa tcaaggccgc cgttcttttc tggcagcaaa
    32521 aggtgtggcg gcccatagga ggatgccgct atgctgtttt gctattgcca tgctctaatc
    32581 cacttccacc actcaaaaaa agccttgccc ctccacaggg ctattccggt tatgagtatg
    32641 tactatacca tgtaaatcag cagcatagca aagcattttc tcgcccggta ggtttgcgac
    32701 ctgccgggcg atttttgtcg tcttttgcgg cccggctgtc cccaaaaaaa ttttttgaga
    32761 aattttcaaa aaggaggtat ttagcgggta gctggcggcc ttgtcaaagt cttgttagcg
    32821 gaaagggaga gaaccaccta agtcccccac agaaaggggg tgagaagatg gatgctatcc
    32881 cccgcgacta tgaagcgcgg tgcatgttcg atgctttttg caagacggta ctgcgtaacg
    32941 aggccaaaag ctacttggct gaaatgaagc gccgccgtga ccgtgaagtg tcactaagct
    33001 ctttgtcaca ggcagatttg gacaagctct gtacagtaga tcactacccc agcgacagct
    33061 ttacgttttc gtcgcatggc tatgacctac atatcaacaa tgagcttgtt gccgaagcct
    33121 ttgccgcgct gcccagcatg gagcagagta ttttaatcct gcactgtgtt ctggatatgg
    33181 cagatggtga gatcggcggc cttgtgggaa tgtcccggag tgccgttcag cggcacagga
    33241 caaatacctt atcggagctg cggaaacagc taaaggcgtt gatgccgaag ggaggttaga
    33301 cgcggatgaa gaacccgaac tacaagggca ttgagctttt gcccctttcg gtgatcgagg
    33361 ccgcccgcgc tggggatgcc ggagccgtag agcgcgtttt gcagtattac agcggttaca
    33421 tgaacaagct gtgtacccgg acgctctatg acgaaaacgg ctatccgcat gtgtgtctgg
    33481 acgaggccat gaagcgccgt ctggaaatca agctcatcca cgctatcgtc acgaactgac
    33541 gaaccggccc cggcagcatg aaacggctta ccctttcccc gtttcggtgt tttcccggtg
    33601 gcctgatcgc accttgataa atatgcccgc aagataacgg cggcgcatca tagggcatac
    33661 ggacacattc tgtctgtgcc gagccggacg gctggtgcgc catgatactt ttccctacgg
    33721 gaaaagcgga gcgaggaaca ccacgccgca atgtgagcgt ggcggcttgc gggcgacgac
    33781 gtacaggcag gactatgata ctcccttata gccacagtcc gagcgttaaa agcgtcgcag
    33841 gcaatgggta gggccgcgtg agaaccacgc gggggtgaaa gtcccgtgga gctgtgccag
    33901 cagccgtccg tctatgcccc taaatcacac aaacctttga atttgtgaaa gggggctgca
    33961 ttatgaacca tgatgtgcct atttgggaaa aatacacgct gacgattgag gaagcgtcta
    34021 aatatttccg tatcggagaa aacaaattgc gtaagctggc cgaggaaaat ccggcctctg
    34081 gctgggtcat tatgaatggc aaccgcatcc agatcaaacg caagcagttt gagaaaataa
    34141 ttgacacttt ggacacaatc taatagtgaa aaagggccgc agatatgtta taataattta
    34201 taagcatata tgcggctctt tccgtcatgg aaaggagcat gacgagatgt cagaaaaaag
    34261 acgagacagc aaaaatcgcg ttttgcggtc tggagagagc cagagaaaag acggacgata
    34321 tgcttacaaa tatgttgata cctttggaaa accgcagttt gtttactcat ggaagttagt
    34381 gcctacggat aagaccccgg caggtaaacg ggaggatatt tcactccggg aaaaggagaa
    34441 agaaatccag aaagaccttg acgacgggat agacaccatc ggcaagaaaa tgaccgtctg
    34501 ccagctctac gccaagcaga tcaggcaccg gggcaatgtg aagcacaaca ccaaactggg
    34561 ccgggaacgg ctgatgcgta ttctggaaca agatcggctg gggagctgcc cgattgacag
    34621 tgtgaagctg tccgacgcaa aggaatgggc gcttcggatg aaagaaaagg gcctttctta
    34681 taagaccatc aataacgaca agcgttccct gaaagcggct ttttacaccg ccatacagga
    34741 cgattgcatc cgaaaaaacc cgtttgactt ccagcttagt gacgttctgg acgacgatac
    34801 ggaaccaaag gtgcctctta caccagcaca ggaagaaagt tttctgtcct ttattcaagg
    34861 tgataaggtt taccaaaagc attatgacgc aatcgttatt ctgctgggga cagggcttcg
    34921 catttcggaa ctgtgcggac tgacggacaa agatttggat tttgaaaacc gggtaatcat
    34981 cgtgagccac cagcttttga gaaataccgg cgtcggttac tatattgacg aacccaagac
    35041 ccaaagcggc gtcagaaaaa tccccatgaa tgaggaagtg tatcaggcgt tccagcgagt
    35101 gataaaaaac cgcaaaggtg caaagccgtt cattattgac gggtatgcca atttcctgtt
    35161 tctcaaacag aacggctatc ccatgacggc ggtagattat ggcgggatgt ttggcaggct
    35221 tgtgaagaag tacaacaaga gccatgaaga agcgttgccg aaaacgacga cgccccatgc
    35281 tatgcgtcat acattctgca cccggttagc caatgcggga atgaacccca aagccttgca
    35341 gtacattatg ggacattcca atatcaccat gacgctgaac tattatgcac acgccacatt
    35401 cgattccgca agggcagaaa tggaacggct ggcagcctga cacaaggccg gttttactac
    35461 tccatttact acttttgagg gggaaatggt gagaggtaat aagaacattt gtaagtatct
    35521 aacaatatca aaaatgccgg aaaagcccgg aaacacgggc tataccgaca ttcaagaact
    35581 tctaaaaaga taatctaaaa tcccatataa ttttcctcct aaaaaaatag cacaatggca
    35641 aagatcgtaa gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat
    35701 gccatatcat cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt
    35761 agaacatcga tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg
    35821 aacgtgcagg gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt
    35881 atttttttct cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg
    35941 gatcaacttg tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa
    36001 aactgactgg aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc
    36061 gaagctaata agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat
    36121 cctctaatct tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta
    36181 ggccttttga ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa
    36241 agtttactta agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac
    36301 atctttatcg taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt
    36361 cttatccaaa tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa
    36421 ctcaattttt tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac
    36481 accgttttta aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg
    36541 tttgtaagag accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt
    36601 atgggactca tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc
    36661 ttctttgtca cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa
    36721 atctactttt ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc
    36781 attgaaatca gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt
    36841 caataagtat tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa
    36901 gtataatggt tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat
    36961 ttgaaaaagg gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat
    37021 tcttctatcc aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta
    37081 actcagttga ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag
    37141 tgagtatatt cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt
    37201 tatactccgt ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa
    37261 atccgtataa tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat
    37321 ccttcgggtg acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt
    37381 ttgctggaag ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata
    37441 tcgtcacttt gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt
    37501 tctaatggaa gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac
    37561 gactgaatac ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg
    37621 gaagtagtag aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat
    37681 tctttaacag aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac
    37741 aagcctgcgt aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca
    37801 gtatatcact taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag
    37861 ggaaggttca tgaagataat aactttttta ataattatag ctttctaata tctttcatca
    37921 acttttctag taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg
    37981 atataaatga tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca
    38041 agttcgaaca agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca
    38101 atagcatcta aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc
    38161 aaaaagagaa acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg
    38221 aaaatttttt ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt
    38281 cttaattttt tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca
    38341 catatgtcgg attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca
    38401 ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt
    38461 gatgtaactg aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct
    38521 tctaaaacgg acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat
    38581 ggtctagtca ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca
    38641 aaaagcaagt atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa
    38701 agtgtatatt tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg
    38761 atcgtgaatc tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct
    38821 gctacaaaag cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg
    38881 aatgttcgtg tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa
    38941 gaatatgaag aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt
    39001 tacagtcgaa caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat
    39061 ctagtctgct attatctttc agaaagatca agttatatca ctggagtcac gactaatatt
    39121 tcaggaggta aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga
    39181 tggtaccaat tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa
    39241 aaagaaatca aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa
    39301 attgccggta ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat
    39361 tttgattttt ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg
    39421 gcatccaaaa gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt
    39481 atggatgatt tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag
    39541 acattaaaag agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga
    39601 ctaagaataa taggagatga gctagaatta cgactacttt tattttattt tgtttctccc
    39661 tacttttcag agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt
    39721 cagaagaaga cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt
    39781 tctttgaata gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg
    39841 tgttttgaat atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa
    39901 ctaactttga gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca
    39961 agtataacga tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg
    40021 atgcgtattc gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg
    40081 aaacagcacc tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta
    40141 ttttatggtg agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata
    40201 caagcattag aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg
    40261 ttgtatttgg agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc
    40321 attgttcata cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata
    40381 ttggggaggg atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta
    40441 aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg
    40501 gtgatccatt tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca
    40561 aaaaaaaagc gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa
    40621 aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat
    40681 gtggatgaag aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt
    40741 aacaaagaat ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca
    40801 gtagggctaa taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta
    40861 tttgccatac ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat
    40921 atcctatttg aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa
    40981 gaatcatttt taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct
    41041 attcatagtt ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact
    41101 aaagcatttc aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa
    41161 acgacctggg aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca
    41221 agttttagaa gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc
    41281 tgtctatgcg ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga
    41341 agatcgtttg acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc
    41401 aggaaatgaa aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg
    41461 gatatggaaa agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa
    41521 tttgctgagg gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg
    41581 acaggaatcg taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt
    41641 ttaggtgaag accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt
    41701 taccttgtgt taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg
    41761 gctcgtttcc ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt
    41821 catacgagca atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg
    41881 attgctcaag ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg
    41941 cttgtgggac tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt
    42001 gtatgcagac agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga
    42061 aaggagagtt ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag
    42121 gtcctttgat tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag
    42181 gtgagaggcc aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg
    42241 ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga
    42301 ctcttagatg tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag
    42361 gaaaaagtgg cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt
    42421 tagaacaaat acagccacta atggaaactg atgataacaa agaaacagta aatactgcat
    42481 ctccagataa aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc
    42541 agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc
    42601 aatcagcaag agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg
    42661 tttccctttt gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta
    42721 tggcaccttt agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc
    42781 catttttatc tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga
    42841 tcggtgtaga gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg
    42901 ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa
    42961 aagctgagac agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca
    43021 tcccacgagt tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DAZUMO010000021        41029 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000021 DAZUMO010000000
VERSION     DAZUMO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.021"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACG8ER_001094"
     CDS             complement(38..1189)
                     /locus_tag="ACG8ER_001094"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS5049692.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACG8ER_001095"
     CDS             complement(1202..1639)
                     /locus_tag="ACG8ER_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5049693.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACG8ER_001096"
     CDS             complement(1654..3420)
                     /locus_tag="ACG8ER_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS5049694.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACG8ER_001097"
     CDS             3684..4361
                     /locus_tag="ACG8ER_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5049695.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACG8ER_001098"
     CDS             4364..5491
                     /locus_tag="ACG8ER_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049696.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACG8ER_001099"
     CDS             complement(5691..6131)
                     /locus_tag="ACG8ER_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5049697.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACG8ER_001100"
     CDS             complement(6142..8175)
                     /locus_tag="ACG8ER_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS5049698.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACG8ER_001101"
     CDS             complement(8177..9880)
                     /locus_tag="ACG8ER_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5049699.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8ER_001102"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8ER_001102"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS5049700.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACG8ER_001103"
     CDS             complement(10963..11988)
                     /locus_tag="ACG8ER_001103"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5049701.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACG8ER_001104"
     CDS             complement(12197..13066)
                     /locus_tag="ACG8ER_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5049702.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACG8ER_001105"
     CDS             complement(13525..15417)
                     /locus_tag="ACG8ER_001105"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049703.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACG8ER_001106"
     CDS             complement(15414..17141)
                     /locus_tag="ACG8ER_001106"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049704.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACG8ER_001107"
     CDS             complement(17157..17807)
                     /locus_tag="ACG8ER_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS5049705.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACG8ER_001108"
     CDS             18011..18307
                     /locus_tag="ACG8ER_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5049706.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACG8ER_001109"
     CDS             18479..19099
                     /locus_tag="ACG8ER_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5049707.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACG8ER_001110"
     CDS             complement(19191..20357)
                     /locus_tag="ACG8ER_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5049708.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACG8ER_001111"
     CDS             complement(20369..20722)
                     /locus_tag="ACG8ER_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5049709.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACG8ER_001112"
     CDS             20850..21872
                     /locus_tag="ACG8ER_001112"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS5049710.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACG8ER_001113"
     CDS             21891..22052
                     /locus_tag="ACG8ER_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049711.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACG8ER_001114"
     CDS             complement(22311..22745)
                     /locus_tag="ACG8ER_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049712.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACG8ER_001115"
     CDS             complement(22855..24714)
                     /locus_tag="ACG8ER_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5049713.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACG8ER_001116"
     CDS             24715..24855
                     /locus_tag="ACG8ER_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS5049714.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACG8ER_001117"
     CDS             complement(24832..25992)
                     /locus_tag="ACG8ER_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5049715.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8ER_001118"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8ER_001118"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS5049716.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACG8ER_001119"
     CDS             complement(27739..28437)
                     /locus_tag="ACG8ER_001119"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5049717.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACG8ER_001120"
     CDS             complement(28394..30055)
                     /locus_tag="ACG8ER_001120"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS5049718.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACG8ER_001121"
     CDS             30273..31673
                     /locus_tag="ACG8ER_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS5049719.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACG8ER_001122"
     CDS             complement(31925..33004)
                     /locus_tag="ACG8ER_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5049720.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACG8ER_001123"
     CDS             complement(33090..34049)
                     /locus_tag="ACG8ER_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5049721.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACG8ER_001124"
     CDS             complement(34061..35572)
                     /locus_tag="ACG8ER_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS5049722.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACG8ER_001125"
     CDS             complement(35594..36157)
                     /locus_tag="ACG8ER_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5049723.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACG8ER_001126"
     CDS             complement(36163..37026)
                     /locus_tag="ACG8ER_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5049724.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACG8ER_001127"
     CDS             complement(37040..37936)
                     /locus_tag="ACG8ER_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5049725.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACG8ER_001128"
     CDS             complement(38111..39400)
                     /locus_tag="ACG8ER_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5049726.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACG8ER_001129"
     CDS             complement(39372..40889)
                     /locus_tag="ACG8ER_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS5049727.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11838 a         8590 c         7097 g        13504 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatat
//
LOCUS       DAZUMO010000022        39611 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000022 DAZUMO010000000
VERSION     DAZUMO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.022"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..569)
                     /locus_tag="ACG8ER_001130"
     CDS             complement(24..569)
                     /locus_tag="ACG8ER_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5049728.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(581..1273)
                     /locus_tag="ACG8ER_001131"
     CDS             complement(581..1273)
                     /locus_tag="ACG8ER_001131"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS5049729.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1292..1576)
                     /gene="macP"
                     /locus_tag="ACG8ER_001132"
     CDS             complement(1292..1576)
                     /gene="macP"
                     /locus_tag="ACG8ER_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS5049730.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1589..2152)
                     /locus_tag="ACG8ER_001133"
     CDS             complement(1589..2152)
                     /locus_tag="ACG8ER_001133"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5049731.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2380..3042
                     /locus_tag="ACG8ER_001134"
     CDS             2380..3042
                     /locus_tag="ACG8ER_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS5049732.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3166..4362
                     /locus_tag="ACG8ER_001135"
     CDS             3166..4362
                     /locus_tag="ACG8ER_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS5049733.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4463..5839)
                     /locus_tag="ACG8ER_001136"
     CDS             complement(4463..5839)
                     /locus_tag="ACG8ER_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5049734.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5876..6742)
                     /locus_tag="ACG8ER_001137"
     CDS             complement(5876..6742)
                     /locus_tag="ACG8ER_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5049735.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6831..7856)
                     /locus_tag="ACG8ER_001138"
     CDS             complement(6831..7856)
                     /locus_tag="ACG8ER_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5049736.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7874..8548)
                     /locus_tag="ACG8ER_001139"
     CDS             complement(7874..8548)
                     /locus_tag="ACG8ER_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5049737.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8732..9625)
                     /locus_tag="ACG8ER_001140"
     CDS             complement(8732..9625)
                     /locus_tag="ACG8ER_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5049738.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9699..11396)
                     /locus_tag="ACG8ER_001141"
     CDS             complement(9699..11396)
                     /locus_tag="ACG8ER_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5049739.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11389..12702)
                     /locus_tag="ACG8ER_001142"
     CDS             complement(11389..12702)
                     /locus_tag="ACG8ER_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5049740.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12702..16643)
                     /locus_tag="ACG8ER_001143"
     CDS             complement(12702..16643)
                     /locus_tag="ACG8ER_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5049741.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16752..17210)
                     /locus_tag="ACG8ER_001144"
     CDS             complement(16752..17210)
                     /locus_tag="ACG8ER_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5049742.1"
                     /translation="MKGLKLKILQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17508..18026)
                     /locus_tag="ACG8ER_001145"
     CDS             complement(17508..18026)
                     /locus_tag="ACG8ER_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5049743.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18028..18645)
                     /locus_tag="ACG8ER_001146"
     CDS             complement(18028..18645)
                     /locus_tag="ACG8ER_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5049744.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18642..19430)
                     /locus_tag="ACG8ER_001147"
     CDS             complement(18642..19430)
                     /locus_tag="ACG8ER_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5049745.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(19492..20403)
                     /locus_tag="ACG8ER_001148"
     CDS             complement(19492..20403)
                     /locus_tag="ACG8ER_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5049746.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20444..21778)
                     /locus_tag="ACG8ER_001149"
     CDS             complement(20444..21778)
                     /locus_tag="ACG8ER_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5049747.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21986..22816
                     /locus_tag="ACG8ER_001150"
     CDS             21986..22816
                     /locus_tag="ACG8ER_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS5049748.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23060..23309)
                     /locus_tag="ACG8ER_001151"
                     /pseudo
     CDS             complement(23060..23309)
                     /locus_tag="ACG8ER_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23500..23694)
                     /locus_tag="ACG8ER_001152"
     CDS             complement(23500..23694)
                     /locus_tag="ACG8ER_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049749.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24199..24384)
                     /locus_tag="ACG8ER_001153"
     CDS             complement(24199..24384)
                     /locus_tag="ACG8ER_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049750.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25234..25575)
                     /locus_tag="ACG8ER_001154"
     CDS             complement(25234..25575)
                     /locus_tag="ACG8ER_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5049751.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25575..25805)
                     /locus_tag="ACG8ER_001155"
     CDS             complement(25575..25805)
                     /locus_tag="ACG8ER_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5049752.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26092..26430
                     /locus_tag="ACG8ER_001156"
     CDS             26092..26430
                     /locus_tag="ACG8ER_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049753.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26635..27197)
                     /locus_tag="ACG8ER_001157"
                     /pseudo
     CDS             complement(26635..27197)
                     /locus_tag="ACG8ER_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27929..28510)
                     /locus_tag="ACG8ER_001158"
     CDS             complement(27929..28510)
                     /locus_tag="ACG8ER_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5049754.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28693..29319)
                     /locus_tag="ACG8ER_001159"
     CDS             complement(28693..29319)
                     /locus_tag="ACG8ER_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5049755.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29341..30672)
                     /locus_tag="ACG8ER_001160"
     CDS             complement(29341..30672)
                     /locus_tag="ACG8ER_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS5049756.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30827..31228)
                     /locus_tag="ACG8ER_001161"
     CDS             complement(30827..31228)
                     /locus_tag="ACG8ER_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5049757.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31314..31520)
                     /locus_tag="ACG8ER_001162"
     CDS             complement(31314..31520)
                     /locus_tag="ACG8ER_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049758.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31615..33117)
                     /locus_tag="ACG8ER_001163"
     CDS             complement(31615..33117)
                     /locus_tag="ACG8ER_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS5049759.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33135..33590)
                     /gene="argR"
                     /locus_tag="ACG8ER_001164"
     CDS             complement(33135..33590)
                     /gene="argR"
                     /locus_tag="ACG8ER_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5049760.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33688..34305)
                     /locus_tag="ACG8ER_001165"
     CDS             complement(33688..34305)
                     /locus_tag="ACG8ER_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5049761.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34526..35899
                     /locus_tag="ACG8ER_001166"
     CDS             34526..35899
                     /locus_tag="ACG8ER_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5049762.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35974..37086
                     /locus_tag="ACG8ER_001167"
     CDS             35974..37086
                     /locus_tag="ACG8ER_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5049763.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37158..38795)
                     /locus_tag="ACG8ER_001168"
     CDS             complement(37158..38795)
                     /locus_tag="ACG8ER_001168"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS5049764.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38963..39571)
                     /locus_tag="ACG8ER_001169"
     CDS             complement(38963..39571)
                     /locus_tag="ACG8ER_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5049765.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11366 a         8434 c         6885 g        12926 t
ORIGIN      
        1 ttttatcaaa tcactttgtt tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt
       61 ctcaatgccc attcatgtcc aacgggatcg aaacttgcca cggaatcttt gaaaatatgg
      121 agtttgtatc ctagattata cgcatcgact gctgtatgca acacacagat atctgtacat
      181 acgccggtca aataaatatc tgtgatctgg cgttctcgca aacggatgtc tagatctgtt
      241 ccactaaaag cggaataatg ccgtttgtct atccaataaa cattttcctc ttgctcatag
      301 tcttggtata aactttttaa agagccgtac aattcccgtc cacccgttcc tatcacatta
      361 tgcggaggaa ataagcggtt ctccggatga aaggaatctt ttggatcatg cgcatcaatg
      421 gcaaaaacga caaaatcctt ttgatcaata aacttctttg tatgagcagt cagctttttt
      481 tcaattactt gtcctgctgt cccagtagtc agctttccat cctctgcaac aaaatcatat
      541 gtgtagtcaa tcgagatcaa tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa
      601 attcgatcac catttctgct gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt
      661 gtgtcgccga gtgatctgct gtgtcgctca ttgcacgtac gatcaaaaat gggatattga
      721 actgacgagc tgtttgtcca acagcggctc cttccatttc gcaagctaat gcttcaggga
      781 aattcgttaa gatttctttg attttatctg gcgagtcgac aaatgtatca cctgtaacga
      841 tcaatccttc ttttgcgttc aaattcgttg cttttgctgc tttaaccatt tcactgcgca
      901 aatatgtgct tgcttcataa tatagaggca ttcctggtaa ttgtcctggg ttgtagccaa
      961 atcctgttgc atctgcatca aaataagcta ctttgtctga tataacgatg tcacctactt
     1021 gtaagccttc accgattccg ccagctgaac ctgtattgat caccatgttc acgccgtatt
     1081 gttggatcaa cagacttgtg gtaatagatg ctaagacttt tcctataccg gaacgtacga
     1141 cgatcacttc gtggttacct aatgaacctg aaataaataa agcgcctgcg cgttcccatg
     1201 ataatggttc gcttaggttc tctctcaaaa tttttacttc ttcttccatt gcgccgataa
     1261 taccgatttt catcttttta attccttctt tctatataaa tgcgattgct aaaaaaacca
     1321 cacataacat taggataaca ataataagag atttcatcaa aaaagagttc catttggtcg
     1381 tcttttcccg ttcgctgatc cttgttttcg tgattggttt attctttttg ctttctttac
     1441 gataaaagct ggcaatcttt ttttcttctt gctgataagc tgtttctgct tttcgctgtt
     1501 tttttaatga ctcgcttgct tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa
     1561 gcggtccttt ggccataagc cgatccctct actttccttt ggtcaatagt tcccagtatt
     1621 gaatcgcata aatcgtcttt gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct
     1681 cttcaagagt tagatgatac aactccagta cttcgtcttc atcttgtgct aaaggattct
     1741 cgaccttctc taccccttgt gcatgataaa tatgaaggac ttcatttgca aaacctggtg
     1801 aaagatacat cgaagtaaga tggctcagcg acttcgcacg atagccagtt tcttcttcta
     1861 attctctagc tgctgtcatt tcgggatttt gtccttctcc tggatcgatt tttccagctg
     1921 gaatctccaa aatcactttt tctaaaggtt ttcgaaattg tttgacaaga agcagacgat
     1981 cttgttcgtc aaaagcaatg atcccgacac cgccaggatg aaataccagt tctctcttcg
     2041 aagttcctcc atctggcaaa cgaacttcgt ctacagcaac atcaataatt tttccgctat
     2101 agatttcttt gcgactgatt gttttttctt caaattgctc attgttcagc atatcttctc
     2161 ctgctttctt tgtaatcata actgcttttt tttcattttt caatttttct caaaaactcc
     2221 ttattagttt accatagata aaccttagat tttctctaaa tatagaaaat ccttctttag
     2281 tccgatactc tttctagtag tccgtatgct aaaataagta tgtaatcgaa taacgaaaag
     2341 aattcaatac tcaacgataa atgacatgga gggtttatta tgataaaacg aaattggccg
     2401 tggcttctag cattggtctt gctatttctc tttgtccgaa acaatagttt ttcttttgtt
     2461 ctaggacttt tattgatagg tgtagtttta gttttgtggg gactctttgg ttcgcgcagg
     2521 aaaaagggaa aacaagaaga aattcctgca ctgactgacg aacttgaaac tcactatgaa
     2581 aaaagcggta tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa
     2641 aatgagatca atcaattgca aatgaatatg cagcagacag ccaaacttaa agcaatcgat
     2701 ctccgccatg atccagtcaa agcagcgaaa gctttattca aagaattggt gaaagaacca
     2761 aaccgactgc atgaagcaag tcagtttctt tatacgcatc tgccgaatct agttgatttg
     2821 acaaataagt ataatgagat caatgaccat gagatcaaaa acaaacagac ctatgaaaaa
     2881 ctagaagaaa gcgcgcagat catcgaccag ctgtcagctt taatcgcaca agattatcaa
     2941 aaattcgttg ctgaggatct agatgatatc gacgtagaga tatctgttgc gaaacagagc
     3001 ttaaagcgtg ataataaata tgaaccagaa caaaaggatt aactcttttg ttcataaaag
     3061 aagactcgga acattcagat gatgtctggc tgtttcgagt actttttcta aagtagagac
     3121 attcatgtaa gaatcgagaa cagattcaac aaggaggaat tttttatgga tcataaaccg
     3181 actgaaacaa aagatgttac accagttaac gatacacttg aagatttatt gaacaacccg
     3241 ttttcaactc caacagatac gctgaccact tcacaacaag cggagattga tcagctccaa
     3301 gagcaacaga cagctgctcg tttaattgac aaactaccgg cggaacgcca agaacaagct
     3361 aaacagctag cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag ctacggatcg
     3421 gctgcccaag caaaattaag tgaattctca caatcgatgt tgaatcatgt gcaagcacaa
     3481 gatattggtc ctgtaggaga ttccttaact gaactgatgt atcgtctcca agaagccaat
     3541 cctgacgaac tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa
     3601 caatcgatct atgaagtgac agcaaaatat caaaagattg gtgcacaaat tgacaagatt
     3661 tcagtgaaat tagataaaga aaaagacggt ctgctaaaag ataatctgat gttagaacag
     3721 ctttaccaga aaaacaaaga ctactttgat gcactgaata tttatatcgc tgctggagaa
     3781 ttgaaaatgg aagaacttca aacaacgatc attccagaag cgatgaaaag agcagaagaa
     3841 actggcgatc agatggatgt tcagattgcc aatgattaca cgcaattcct agatcgtctg
     3901 gataaacgta cgcatgatct tcgcttagct agacagatca cgatccagca agcaccacaa
     3961 atccgtttga tccaaaacac aaatcaggcc ttggctgaaa agatccaagc atctatcgct
     4021 acagccattc cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa
     4081 gatgcagtaa ctgctcaacg ccaagtctct gaaacaacaa acgacttatt gaagaaaaat
     4141 tcagaaatgc tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg
     4201 gatatcgaga cccttcaaac cactcaaaat gatttgatcg aaacaatcca agagacattg
     4261 cgtatccaaa aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct tggacacatg
     4321 gaagaagatc tgaaacaaaa actgttggat ttaacccaat agatcacctt tcgcttgtta
     4381 ctaaaacaaa cgcccaacaa cagtttatta caactgttga tgggcgtttt tcttatcttt
     4441 cttctaactt ctccattgga gtctatttct cattcatctt gctgtgtcca tagccatagc
     4501 caaaataaat gatcagtcca ataaccatta cgataccgaa cacgatccat gtcacagttt
     4561 gcagacgaag catcaataca atacaagtca agatggagac gattggcaat agcggaacga
     4621 atggcacttt gaattctcca gcttttggtt tgccaaacat ttttctcaac ttcagtaagc
     4681 ctagactaac aagcatcaaa taggcgagcg ccatgatatt cgttagttcc gctaatacat
     4741 tcaatgggaa aaatccggca aagaatacag ctgccacacc agcggcataa gtcgcttttt
     4801 tcggtgtacg atgcttttga tcgatttctg tcataaactt aggaagtaag ccatccttgc
     4861 tgacagcaaa cagtaaacga gcaagtgaat acatcatcga gatcgttacc gtcagtagcg
     4921 ttaaaattgc ccctacagcg atcaccgcgc ccactgttcc atgtcctaca tacctcattg
     4981 caaaagccac tgggtcattg actcctagtt cagtatacgg aacgattcct gtcaagatca
     5041 gcgtcacgat gatgtaaagg acagtagcaa tcagtatcga accaatgatt cctcttggca
     5101 catctttttg cgggtttttg acttcctcgg ctgccattgc aacggcatca aatcccaaga
     5161 aagcaaaaaa cacaagagct gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg
     5221 gctgccagtt atcaggttta acgaaaaata ttcctacaat gataaacaac gcgatgatac
     5281 caaatttgac atataccatg gcattattca aacgtaatgc tttcttggct tcaagcgaca
     5341 cgatgtaagt gacgaatatc actacgagca ccgcaatcaa gtcaatgtat gtaccatttt
     5401 ccggattgta cccgccagtt aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac
     5461 cttgcatata gccagaccat ccggaagcaa cagaggaaac cgctagtaaa aattccccga
     5521 tcaacaacca ccctgtcagc caggcagcaa attcaccaaa tacaacatac agataggcat
     5581 atggcccacc aatcactggc actcgcgaag caaattctgc gaaacttagt ccagacaaaa
     5641 tgacaacgat tgcagctaag acaaatgaaa gtgacagtgc tggaccagca ttttgattgg
     5701 ccgcaacccc tgtcaccaca aatattcccg taccgatgat cgcaccaatt ccaagcatga
     5761 tcaaatcaga cgtttttagt tcttttttca tctcactcgt atgattgaga ttgatctctt
     5821 tcttacgaaa caaatccatt gaagttcctc cccattttag gttggaaaac agcgattaaa
     5881 ctgttttcat ataccctaaa aggataccac ctaaaatgac taaaaggcaa ccaacaataa
     5941 cataaatgag ctcttttttc gtttttcttt cgccaaggat gaaaatccca cctaaagtag
     6001 agataatgat ccccatttgt gaaagtgaga agccaacagc taaaccaagt gatttgactg
     6061 ttagaagcat gcagatgttt cctaagcccc ataataatcc acagatcatg tttttccaca
     6121 caaactgatc gactttaact ttacggaaag caaagaagct tgcgcctaag atcattccga
     6181 tactttgagg taagataatt gacatcgcat ccaaattcgc ccagttgacg atgatggtat
     6241 atacaccata accaatcgta gagaagatca atgctctaaa acccttacca aaatcaagca
     6301 ttttattatc tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc
     6361 ttacaagaag agctaaagca ataataccgt atacataatc tttcgtttgt gtccattcat
     6421 ggaagaaaac ggctcccgca atcgtattca aaattaattg aaaaccagta gacaatggta
     6481 gccctacaga aacacccatg tacttcattc catggaactg tccattttgt ccaacactcc
     6541 aagctaagcc ggataaaaat ccgatcaaga aaatccaagg ggtgattaca ggactaacaa
     6601 cgaaaaagac aactaaagaa aacagaaatg caccgatcgt cattcctagt gtttgttgat
     6661 tggcatcgcc accaatttta ccacttacta aaccaatact accccatgcc aacataggga
     6721 ctaatgctat taaaatttcc ataaccaatc tccttttatt tcacatgata agaaacttat
     6781 cagcgaaaac gttaacaaca caagcacttt ttttcgtttc taacgatttt ctaacgtttt
     6841 tcattataaa aatttaagat tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt
     6901 gattcagccg tcaaatggat acctaatttt tcggccagat tttttgctaa ataaagtcct
     6961 aatcctgttg aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc cttatcaaag
     7021 atccgtttca agtcttccgc tgggataccg atccctgaat ctttaactga aaggctgact
     7081 ccttgcgctt ccttttctat gctcacaatg atgtttccgt gatccggcgt atatttgatg
     7141 gcattactaa gcaattggtt gaagataaaa gcgatccatt tagcatcggt caaaacagta
     7201 tgatcctcac caacgatctc gtagcgaata tttttttgga taaaataatt tgcctgactg
     7261 cgtatcacag gctgaatgat tccttttagt ccatattctt ggatgaggta gtcccgcgag
     7321 aaactgtcga gtcttgcata atacaaaact tgttctacgt attcatcgat ttttgataat
     7381 tcattttcta gtaatgtata cttttgatcg tcaatatcga actcgatcga acgaactagc
     7441 aatttagaag cggaaagtgg cactttgatt tcatggaccc atgagtcaat ataatctttt
     7501 tggtcttgtt gattactgat tgctgattgc attacttctt ggtgttcacg gatcagctga
     7561 ttgatatatt cttctaccag tttttcttct tctgtcctcc caccactcaa ataattttgc
     7621 aaaggggaag ttttttgtat gtcggctaat tttccccacc atcttctctt gctcaagtaa
     7681 ccacccagca gataaaaaat cagaaagacg ccctcaatca aagctaaata agcaattgtg
     7741 ctcaaatcaa ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa
     7801 aacagccatc ccaagatcaa tgtccactga tctttcaaat atttaaaaaa attcatctta
     7861 accgctcatc ctttcaggga acgatatatc cctgtcctac ttttgtttcg atatatcctt
     7921 ggatacctgc ctgttcgatt tttcggcgta accgattgat gttgactgtc aatgtattgt
     7981 catctacaaa tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga cttaagatct
     8041 ttccattgtg cttcatcaaa ataaataata gacgatattc atttttgctc aaatcaatga
     8101 tctctccgtt gatttccata cttccgttgt caacattcaa cgtaatgcca ttgtggctca
     8161 tcatttcact agataattct gtgtagttgt aagaacggcg aagtaatgca ttgatttttg
     8221 caatcagtac atcgatttgg aaatgttttg tcacaaaatc atctgccccc atgttcatag
     8281 ccatgatcat atccatattc gtattcctgc tggagataaa aatgatcggt actttcgata
     8341 cttcacgtat tttttgacac caataatagc catccaaaac gggaagatta atatctagta
     8401 atacaagctg tggttcttct ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc
     8461 caaaggtttc aaattgccat ttctcaagtt cctccatgat caattgtcga atgatctctt
     8521 cgtcttcgac taccatgatt tttgccataa aaaatccctc catctttctt cttgctacta
     8581 caatatcaaa gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc attttagcaa
     8641 acgttaaaaa aaactggaaa tatgcgattt aatcgcgttt gtctcttcca agcagcttaa
     8701 atttattatg ttattcttaa taattggtct attatctgtt atttttcttt cgttgttttt
     8761 gtcttttaaa ttttcgataa caccaataac cgaaaagcgt tagcactagc aatataccta
     8821 gaaggagtat aagtaatcga taaagatggt atttttttgt ctgttctatc tttgaagaga
     8881 cttcttctgt aaagggaact cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt
     8941 aaggtgtaca agtgataagt gtgactagat cctgcttttc tctaattgct aatgtatttg
     9001 tttttgttgg aaggacaaca attttctcaa cgacttcata agcgagcatc cgaccataca
     9061 cttcaagata gaatcggtca tcttgctcca tttcttcaag atccgtaaag agttttttct
     9121 cagcaatacc gctatgggcg gtaatcacgg aatgtgtact ctctcctcca gttggaaaag
     9181 aggtgccttg caggactgtc gctcctctat ctaacaaaag atcattcgtt tcatcaaata
     9241 aaggcaagct aacattgatt ttcggaatat agatggctcc gatcatatgt tgttcgtaat
     9301 attcttttcc tggattttgg acatttctaa ccgcttgctc aaaaggatct ttgacttgtc
     9361 ccattcctgg gattactgat gtatgagcaa gcgtctgatt tttcttttca tgcactgcta
     9421 attgttgctc atgtttttct tctttatcgc gttcaagctg cttttgataa ttctcgatcc
     9481 ttctttgatc caaataattg ctgagagcat ctgctaaaaa tggatatgta aaaactgcag
     9541 ctccggcaag aaacatcact gccataaata attgtaataa aagactgatt tgggtttgag
     9601 gtctattacc tattttcttt ttcattttct tcaccttctt atttaaaaaa actgcagcaa
     9661 cctttataaa accgattgct gcagcttatt tttgttgctt atacttcaga cttcatttta
     9721 gatttacggt accagctgta cgctccaatc atcaaactta ttccgattag taagaatgcc
     9781 aagagcccgt tcccaccagt agcaggaagt gacccgcgtt tcatattcgg tactctttct
     9841 ggccctggat tttctactcc tacgatctga atttcttcat tatagcttcc ataagagatg
     9901 ataaattcag taaaggctgc ctcacctgtt ggaagtacat aaccttctgg cgccatggtc
     9961 tctttcattt gataggttcc ataatctaac ccttgaacct caaagtatcc atttccatct
    10021 gcgacaaatt ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc tcctgattca
    10081 taaacagcta aaggcgactc ccctttataa aatattgcgt attcagaacg gtcttcattc
    10141 cacaattcga atcttgcatc tttaagcaat tcactgcttg atgcatcata tttttcaaac
    10201 tttcttccac ctgttgtgac tttctctggc ggctctactc cttcttcata atcaaattca
    10261 tcatttcgtc cgatttcaat aaccgctgtg ttgttatgga aatcatccgc ttctagttct
    10321 gtggaaacct tcaaattata cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta
    10381 cctaaagtat ctactttggc tactccattt tcttctgtca aaatcgtcac gacaaacccg
    10441 ttgcttacta cttctactat ataatcttgt ccttttgtta aaatcgtctc gccggctcgc
    10501 acttctattt cctgatcata ataagttaga ccaactgtag gcgtatctgt gattttgtaa
    10561 tttttgataa cagcgcttcc gttttcaagg acagaatcga tgaaataagg aataggtgct
    10621 gtgatcgtgt aagaagccgt ttttcctctt tctagatcaa tataagaaat cgtttcttgt
    10681 tctccagctt cattaataat cgttacttgt ctaaggtcgc tttcctctac attcatctgt
    10741 ttatctgctt ctctgatttc gtttttcgga tacaaatgca catctgtatt gtatgtgtct
    10801 ttccatgtag aaccatccca cacgccatcc accatatcag gcatcatcac tggcatcata
    10861 acaaccattg gagacgcgat tctggaaata tgagctggcg aataagtttc taagaaaaga
    10921 tagactttat ctctagaaga aggtgcttct tccgtcactt ggacggtaga gaataccgct
    10981 tctccagctt cattcgttgt ttgtttgtca atacttgtca gtcgatattt taaaaaccaa
    11041 ctttctgtgt gccgtttttg aatccattca atcgcttcgg cagaattaag accgtcttct
    11101 cgttccggtg ttaaaggatt atctcgaatc aaatcgtaat atacttccgt tacgtcaaac
    11161 aaatcaaaat cgactcctgg aatattttct tcaccaaaat ccgtagaaac caatccgcta
    11221 ttcggtcgtt cttccggaac agatgagaaa ccttttttgt gtaatgtaat cgtcacttca
    11281 cttggttttt gtgcgacttc ttcggctttg actagttttc cgttcccgaa aatccctatt
    11341 cccgcagtaa cgagtaacag tacactcatt cccgtgacaa tcattttttt attcattttt
    11401 ttctccccgt tcttttttta ttaataaaac agcacctaac aataaaagaa ttcctagtaa
    11461 agaaatcaac gttcttgctt cattcgtttg cgggaatacg gaccattttc ctgattgacc
    11521 tggattgctt ggaccactcg gctctttcgg tttgtccgtt gtttctttta cggtctcgtt
    11581 ataaacacgt ggtgttgcgg attcataggc agagactgga actttcccgg attctaattc
    11641 agccatctcc tgtccgttaa ctactacata ttgtggttgg ccttttgcat ccacccaaaa
    11701 ttcagggatg atgacctcga ttctcttact tgcttcatcc atctcgtaac cttccacacc
    11761 ttttaattct tcaaaataat aggtgccaga aggcaagaaa cgttgtcctg tcgtaacaag
    11821 gccctcttta tccgaaatga atttgcttac atttttgtca ttcaaaggat cattgtctgc
    11881 tggtttgatc cattgatttt tcaagtcatt agcaggagcc atatctaaat aatatttccc
    11941 cccttctatc atctgatata gagcaaaaac agcaccttct aatggttttc ctttttcagt
    12001 tgttcctctt tgacgtccat acttgaagaa gtatggatct cttagataac cgacattttt
    12061 aggatagata tgaatctctt gcagttcctc ggttgattct gtcgggtgat aaattggcaa
    12121 aatagcagca attgggatag ctgtctgatc gatatcaaca tccaatccaa cttctggatc
    12181 aatcgattct tcgaaaatca aataaactgc atcccgatct tggtttttct taggtgcttt
    12241 gatacgtgca atcccgtctc ctaatccgcc aaccgtatct ttcccagttg ttaccgaagt
    12301 ccctatttgg acaagccttt cttctgtgat tagctgacgg atattgtttc tgttcgtttc
    12361 agcgatttgc tggacgaatt ctttaggagt catgttttca gcagcttttt tttcatgata
    12421 ataatcggtc atgtcgtaaa tggcaaatgt cgctccattt agtggcaccg tctgtgaaat
    12481 gatatctgct gtttcccctg cttcctcaga gatagccaag ccatcatttt catggtattc
    12541 aaattgatcg ttataatcga tatctcgaat catccgttta tgcaacacga attccacgac
    12601 atcttcccct gattcttctg ccgacatcgc atgtggaaga aggactgctg caagcggtag
    12661 taaaacaagt aattgaagca tcaaacacaa cagctttttc attaccttgc tccctccttt
    12721 ttagattgtc tgacaaacaa accataggct gctcctgcaa gcgccatgaa gaatgcagca
    12781 agcaggatat agaataatcg tccggatcct cctgtagcag gcaacggatt ttcaggatcg
    12841 ttcccgacac taagctgtaa tctgattcga tttgtctcca aaagttcata ttctaactct
    12901 tgctgtagat caggcgtctc tgcttcttcg tttggataat acgaaatctc aacttttagt
    12961 gtacccgctt cttttcctgg aactacccga tcattttcta tgtcaggttc ttctcgataa
    13021 ctaatttcta aaataaagta acctggaagt tttctatagc tgtcaggtga cttcacttcc
    13081 ttgatccgat agaccccttc caacaagtct gaagcattga atagggaaga atcgtttgga
    13141 tctggtgcca agcctccctc gatgatttca tactctcctg cactattttg tttctcaatt
    13201 gaaaattctg cgccttcaag acgtcggcta ttcatactgt caattttcaa aagttccaaa
    13261 tcgattggcg ctagttcatc ttctaaggtt acttgtagta caccatcgat gatttcatag
    13321 ccttccggca agggagcagt ttcgtcttcg atgaccgtat cttcaatgat aaacttacct
    13381 tcttcattca gtgcaaactg gaacacttga tcagatagcc gatacccatt tggtgcgaca
    13441 gattccgaga cctcatattc accaatagac agatgcagag gttggtcgta atttaaatcg
    13501 acatactttt ctactcctac ggcacgagaa tagaagtatc cgatcccagg caaacgtttc
    13561 gattcttctg gttctaacgg attaccagtg tatgctcgat acagcgggaa ggaagaatcc
    13621 cccactttcc tgatatcgaa acctgcacca tctaatgtat ggccttcatg gtctaatttt
    13681 ctaactcgga tctcccgata atcaaacgta ttgtaagcag tgaatgctac ccataaacga
    13741 tcctcttcat ctgttgtata cgtgagatat tgccaatttt cttcacctgt ttgcttgagt
    13801 tggatagtcc ctatagcatt atcgtaaagt cgcaagatcc atgtgtcatt ttcatcaggt
    13861 tctttgaaac cttctgggtt ctggctctca atgattttgt attctgcata gttggtagtt
    13921 tcttcactat ccttataaac aaatgaatct ggctctccga ttggatgaat aactttctca
    13981 ccatccacga ttacatattt cccgtcttca tctactgaat agaacgtgag tctccctttc
    14041 tcttcctcta cttcgttcac accagaaagc cctttcgcta ctggatatcc gatttccagt
    14101 ccgttaccat ctgtttcata aagttcaaac tctgcgccat tgattggcat attactgcct
    14161 tcaattagtt tattgatatt catgtggatt ggcttgaatt catttcgcat ttccagtttg
    14221 acatacaact catcatactc tgtgccttct atgttaggct cttgtacaac agaaagcttg
    14281 tgatattctc cttcataata ggaattcgga tctttacctt ccggtccgcc gactctcgaa
    14341 ccagatgtat attcatctac aagctcgtag tgacctaaca tctcatcttc gttggctgca
    14401 tattttttga tttggaattg atacgtctca tctactaact gataacgtgg tggtccagcg
    14461 atctctctga tccaaaatat gtcgctgact gctccagttt catctcctaa aatcggcatc
    14521 ttcgtttcaa agatcgctat tccgtccgtg gtagttttac ttgtttcgat tggttcggct
    14581 tccccactct ctggattttg gtcatataat gcaaattcga ctccagccaa cggctcgcca
    14641 ttttgaccct tcttgctgac ttggaaatta cggcctttat gttcgtaacg aatggaagaa
    14701 gatgggaaat acatccgtcc agtagtgata tcattctcat aatcctccag gttctgaata
    14761 taggccatat tgttcactgt gcaaaaattt ccgtcttgag ctcccgcatc aagttttact
    14821 tggtacttga tcctgaccat ttccccttca cctaaagaaa tcggagaaaa tttataagct
    14881 tggttttccc gtccgacata cgttgtcgga gtcgccacta cttctggtgg ggcttgtcca
    14941 tgacgttctt catcccatat ttctggctct tggtacttca tgccgttttc tcctggatct
    15001 agatggtaaa actgaatatc tgccgatctg ccagctccaa atggtgagac attaaaacca
    15061 gtttcatcat aaagtgtggc ttgctggatc gttctcttga aggtagaaga gatatcctcc
    15121 aaggctgtaa caatgtcatt cttgttgttc gcattgtaat atcgcggtgt tacgccatct
    15181 tcttctaaat cagcaatatt tttcaataca tttcgtccaa tagcatctag tcgttgtctc
    15241 ccgctaacgt ttccatcgat tcctaaacca atggagaaga tacttggcgg gttctcttga
    15301 tacttgtttt tcagccaatg atgataagca actgtgttga tcgcagatga cttttcttgc
    15361 gaagtaggac tgccattcgt attgttatta gaagaaccat caccaaccca gtgagtcgct
    15421 ttattatctc cttcttttac ctctccataa cgatagtgta catttacttc atcttctgga
    15481 cggttaaagt cagatgcata ggtaactgga tgagtaaacc ctgcattaga agtattccca
    15541 gaaaactcct caaaatttct gaacaaaacg tcattatctt cagcaatctt atcttccatc
    15601 acagtccagt tgctccaagc tccattagtg ctcgtacgat aacttgactg aatcggtgca
    15661 taactgaagg tcggtgtccc atctcccaca acgattagga ttttttctgg atttctattt
    15721 tctccgccat tgtctttata caatgtttca tagccatttt taagccctag tgtaagcgga
    15781 gttcctccga ttggtgtacg atttaatgct gtattctcac ttcccgtcaa acgattgata
    15841 tcatttgtca atggaaagat gtccgaactg atttgctcat ggttattgat agctgtacta
    15901 ttatggtaaa aattcaccat accgattcga atatccagac tcgggtcaga gagcagatct
    15961 ttactcattt cgtttactgc ctcaatcaac gcagcatttt tagtttgact attggtccca
    16021 gcggttaatt cactcatact cgctgactta tctagaacaa aaacgatatc gattggactg
    16081 atttcctttt tctcgccccc gatgacatct agatagacat tgtaaagggt tgttgcatct
    16141 tcaggatgta gatcagatgc tgtgctgctt ggcagaacag gttctaccca ttttttgatg
    16201 tacgccccgt tataatccaa gtaactgttt tcaaaattcc gttcatttcc ccaaagctga
    16261 acggtttgag ctggaacgtt tctatcttct tcgcctgcag ttgatccatt attagtagca
    16321 tagtcgtaat ttcgccagtt cgctcccccg tttgtataat cactatcatc gatcaagttc
    16381 tctccgtcat atggcgagcc tttgtctcct tgatagcgtt cattgctttg tgtccaattt
    16441 cctaacactt ttgtatagtc attcgttgta aacttcgggt agcgatgctg ttcgttttca
    16501 acttctccgc taggatagat atattcatag gggtcttgcg tagctgttgg cccaagacgc
    16561 tccaaaatac cgttgaataa ctggcgttga atgcgctgga gtgcttgacc gctgggtacg
    16621 attggttcgt ccagcttttc catctcattt tcttcgctac gttgaatttc agcggaatct
    16681 tctgtatcag tttcattttc ttgtaattct tggttatcta ttctttcatc aaccaccact
    16741 tgaagaggta gttacccttc gttcacatcg gggatctttt ctgtttcaaa aatcaatgtg
    16801 ccggtttctg cttcttttga ataaactttt ccgacatacc attcttgacc atcgatcgtc
    16861 tcgactttta ggtcttctcc atcttcatca taggaggata gacctgttcc acgaacgttt
    16921 tttacggtac caatcccggc ggctgccgca tcgattgcta aacgtattct accttcattg
    16981 gcactttcct ttttttcaaa agccagatgc cattcaaggg tttcttcttt ttcaatatac
    17041 ttcccttcaa tttttgcata gtcctcatct aagaatacct catcctcctt tgtttctgtt
    17101 gactcagcag ctacatccag ggtcgtcata tgatagactg actggaaata tggtccaaat
    17161 agcaaactga cagttactaa ctgcaaaatc ttcaatttta accctttcac atccttatac
    17221 tcctttcaaa aatatttgtc ttctttcaca acaatcaaca aaacaatgcc catctaatta
    17281 taatttaatt taattttaat tttaaatcaa atgatatcct ttttagttaa aaaacaccaa
    17341 ttatacaaca tttattatta aaattaaatt ataaaatttt ataacgttct catttaacaa
    17401 aacttaagtt attaagacaa atgaataaac aactacgctt tcctcgaaac aaaaaaataa
    17461 agcacaaatg cgcatttacg cactcatgct ttattcgtac aattaattca gataacggca
    17521 gcgcatagga tgccaataac acttcctaaa atagctcctc ctaaaatgtc agtaggataa
    17581 tgtaaaagta aaaaaatcct cgaataagcg atcaaacaag caaataaaaa ggctaatatt
    17641 cctgcaactg gacttaattc gaacaacgca gctactgata aaaaggctag cgctgtatgt
    17701 cctgaaggaa aagaagaagt cttcggttta cttgtgtaaa ggacaagccg acgatttttt
    17761 tcataaggtc ttttccgtct tgtcgttttt ttgataaaaa gcaaaccaac aaaaaaatag
    17821 atcaaactgc cgataagaat ggctagacca tattctctag tatcaggtat caagactaaa
    17881 acagcagcaa ggagcaacca aataaaacca ccattagaga tattactgat aaatttcaaa
    17941 aaattgattt ttcgactatc cgttttccgt tgcttggcga acttgttgat aatactatca
    18001 tcgattttag caatcaaacg caacatgtta ttcctccttt ttaagaacct tttcttgcgt
    18061 acgatggata aaaagcagga cttgaaaata aaacaagaaa ctgaaaccta ataaagcaaa
    18121 atagattggt cctgcaaacc agaatgcgac aagaaggatc aacgtgcctg ccaaatagaa
    18181 aagcgcgctt ttgaaatcat taaaaaacaa gaaaaatgca gtaatccaag aagcttcttt
    18241 ttgttccagt tgaagcaaga ttttgtaact aaagagaagc gcgcttaata gaagcaaaaa
    18301 caacgtagta tacatcaatg tatagccaat tcccccaccc caatagcgga aaatctgata
    18361 atcgatcaat agaagaagaa aataaacact tgctaaacta tcttgctttc cataaagaga
    18421 gaagttctca cgaaactttt tccagaatag tgaaccgagc tttttctctt catttttttt
    18481 gtaatgataa atcatataca gaactgttcg acttgcagtt cctaatgtga aaacaggcaa
    18541 gacgcacagg aaccatagta gctgtaaatg gataagacgt ggaatccatt caatgatttc
    18601 taagaaataa tcattggctt ttttaccaat cgatcgattc atcatccttt tgaacctcct
    18661 gtcaaatcca tcccttcaat gaaataacgt tgtgcaaaaa agaacagcaa aatcgtcggg
    18721 atcaagacaa gtgttgctcc tgccatcaga taattccatt gtgtcgttgc ttggttctga
    18781 aaaacttgca agccaatctg taacgtgtat ttttcttgat cttggatata taacagagga
    18841 cctaagaaat cattccaagc tgcgttgaag gaattgatcg caatggtaat caaggcaggt
    18901 ttcgtcaatg gaagcatcaa atgactccaa atatacaaat gattcgctcc gtcgatttca
    18961 gcagcttcga tcaattcatt attgatcgat aagtaaaatt gacgcatcag aaatatattg
    19021 aacgcattgc cgaagaacga tggaacgatc aggggcaaat aagtacctac ccagccgatc
    19081 ttggagaata atacatattg agggatcatc gttacaaacc ccgggatcat catcgtagaa
    19141 agaaccaaag caaacatcag ctttttccca ggaaaatcaa gcttcgcaaa tccataagca
    19201 ataaaagaat ttgaaaggac attaccgatc actactagca cagtgatgaa taatgtattt
    19261 cttgcatagc gccaaaaagg aaaggcggca atcgtatcca gatagttttc ccatacgatt
    19321 ttttctggga aaaaggtcgg aggataacga acgatttctt ccattggttt caatgaagta
    19381 aacaccatcc aaagcaatgg aatgatcaaa atgattccta ttactgtcat gaggacataa
    19441 cttagtattt ttttgtttgc cgattcacac tggtcttttt gaaataatcc atcatttgcc
    19501 gtcacctcca gaataataga cccacttagg tgcaagcttc aaattgacaa acgtcagaac
    19561 caacacgatc agaaacagga tcattgacat agccatcgca taacccatat caaaagctac
    19621 gaacgccttg ttccacatat gcagattata aaaaagcaaa gaatttgcag gtccgcctgt
    19681 tccatttttc gtcatgacat aagcttcttg aaaaatttgg aaagaaccga tcgtagaggt
    19741 gatcacatcg taaaaaataa taggagtgat taatggcaaa gtaatacttt tgaactttcc
    19801 ccaaacgcct gctccatcga tttctgctga ttcatataaa gatctaggaa cattttgtaa
    19861 tgtagctaag taaagtagca ttgctcctcc agaactccat attttcatca aaatcaacgc
    19921 aggtttcgtc cattgtggat caaacagcca agctggtcca gggatatgga accagcccag
    19981 aaatgtgttg atcaatcctg cagatggaga aagcagctgc atccataaga tgtagactgc
    20041 tactcctgat aagactgccg gtaaatagaa agctgtacgg aatacactga ttcctttgat
    20101 tttttgattg agtactgtgg ctaaaaacac actgaaaatc gtcgtcagcg gtacattgaa
    20161 gatgacgaaa taaacggtgt tgtataacga tttccaaaat aaatcatcgt gaacgaacat
    20221 tcttttgaaa tttgctagac caatgaaatt catcttggat gtcaaattat aatcggtgaa
    20281 actaccaata actgaaaaaa gcataggccc tagcataaat accaagaacc cgataataaa
    20341 aggcgagata aataaatagg cccatcttgc ttcttttttc tttcggatag ataaacgctt
    20401 caaattttct tgcctcctct gtacctagac agtcaaaaat ggattattga ttttgcttga
    20461 ctaagttttc tactgctttt tgtgcatctg ccaatccttc ttttggtgat tttgttccta
    20521 aaagaatctg gtcaatctgt tcattcacta atgtcgtgta atccggagca tctaaaggaa
    20581 ctggtgtgat caccgtattt ttaaagtttt cgtccgccat ttgatagatc atttttcctt
    20641 tatccgttag gttgtcatta ttcactaaat tctcattggc tgtcttgctc gccatgatat
    20701 caaaactttt ttcgccgaat ttttcttgta cttctggaga agaaagatat ttgatgaatt
    20761 cataagaggc ctcaggatct tttgctccgt atggtacttc caagacgaat ccgccacccc
    20821 acgaccaatg gcctgaacct tcttcttttt ctgggagctg catcacgcca aaatcaaaat
    20881 catccggtgc attttccatc aaactagagt aataattgat attttgggca cgcatggcta
    20941 ctaatccgga aataaatgga tcagatacac cagacccaaa ttcggcttct aatttattga
    21001 tcgtattttt cccataataa tcttgccagt cttggatcca ctccaaggct tccactttat
    21061 ttttagtatc aatcttgact ttttcatctt tgtcaaacca actagtcccg ttatctgcgt
    21121 ttagcgccca tacatcagcc cctaaattcc ataatggata aaaaccgatg cgtgtaaatt
    21181 cttttccttc ttttttatct agtttacgtg ctgtttcttc taattcatcc caagttgtcg
    21241 gtagatcttg ttcactgatt ccagcttctt taaatatctt tttattataa aaaatgactt
    21301 gtgtatctgt attgaatggg atcgcataag gatcaccttc atattctact gtatcccaaa
    21361 gctgcggata aaaatcctta gaagtgtctt tttctacata tttggagaga ttagttgctt
    21421 gctgtgcttc tgctctttgg gcaacggaat ttatgtcctg tacaataaca tcgggtgcat
    21481 ttccagctgt tattgcagat aaagatttag tccagatgtc accccatggc tgatacttat
    21541 aatttacatg gatctcgttt tgtgattgat tgaaatcctc aatgatttct tcgatagctt
    21601 cttttcgtgc accggatccc caaaaggacc agaaatcaat attttttctt ccatcacttg
    21661 ttttttcaga agaatttgct gcacttgaac tggcatcgct tgcattactt gatccgcatc
    21721 ctcctaataa aaacaatgct gaacttgcca gcaaccctgt aaacaataat tttttcatat
    21781 tttacctcca aattttaatg taaccgatgc cggttttaat ataacgatat cattttgttc
    21841 tgaaaataac aatttataag gtatgattct aaaaaaatta ttaatgatac caatacttta
    21901 tataattcgc gagcgctttc tttgaatttt ttaatctttc gtagcagaat gagaatgtat
    21961 taaaaataga ttaggaggaa ttttgatgtt tagacaccag aaagaattgc agtttgaagc
    22021 aaaaccagat cgtccgaatc cattaatcgc gaaatatctg caggagctga tcggcggcca
    22081 atatggcgag atgagtgtag ctatgcagta cctgttccaa ggctggagct gccgaggaga
    22141 agaaaaatat aaagatatgc tgatggacat tgcaacagaa gaaatcggcc acgtggaaat
    22201 gttggcgaca atggttgctc gtttattaga gggtgcgcct gttgaagatc aagaagcagc
    22261 gatgcaaaaa gatccaacaa tcggtgccat tatctctgga atgaatccac agcacgcgat
    22321 cgtagctggc ttaggacctc gtcctgctga cagcgtgggc aatccatgga gcggtgccta
    22381 tatcattgcg agtggcaact tgttagcaga ctttagagct aatttaaatg ccgaatcaca
    22441 aggaagactt caagtagcac gtatttacga aatgatcgat gatcgtggcg taaaagatat
    22501 gctgtctgtt ctccttgcaa gagatagcta tcatcaaaat ctatgggaat gtgcagtgaa
    22561 agaattagaa gaaaaagaag gttcaatctt agtcccaagt actttcccgc gtgacaaaga
    22621 acgaaaagac attgcttatg acttctataa cttctcagag ggagaccaaa gtagtgaagg
    22681 ttcttgggct agtggcaaag cactggatgg cgaaggcgaa tatcaatggt tgaaagaacc
    22741 taagatcgaa ggcgctgcac caaaattaga accagtaaca ccaaaaatgt atgggacacc
    22801 tccattcaag aaataaatat ctacccgtag aaacacccgt tttttgttcc acacgctttg
    22861 tgagcaaaac tgactaaagt cattaatcat aaccaccctt caaaagggtg gttttctcta
    22921 gagctataag ctcaaatcac cggccagcgc ctaaagacgc tggctttcat attttatgtt
    22981 caagccatat tgcctttgcc agttttgccc acctcttaaa aggtttatgt actactcctt
    23041 agcccttaaa aggatcgtct tactctcgta cactcagctt atctaacgca ctatcatgtt
    23101 tttcttgttc ttgaatgtac tttttgatgg tcgcttcatt ttgtcctact tttgtcacat
    23161 agtatccttc cacccaaaag tgtcgatacc caaatttata ttttagattt gcacaatctc
    23221 ttgatgatct cctgaatatt ggctcgatat tgattataaa ttatttttct tctatacttt
    23281 ggtgtgaata caatgtggta tttacacatc tactttgtac gggctaaact atttcccttt
    23341 attgctccac ccgcatagca agtggttttg tatttcaatc gctcggcgcg ctcaattagg
    23401 tctaaagaca ataaatctaa aaacccgctg cttcactgac agaaacaacg ggttttcaga
    23461 aaaaaatgaa atgaaaaaga tctcttacgg caatctattt tatttttttg attttttata
    23521 aatggaaaat gcttcaagaa gtccaagaaa taaagccgta taaaaacaag aacctatgaa
    23581 atctttattt attagatatt caacagaaat cccgataaca ctagccaaca atatatttat
    23641 cagaagtgta aaataaggat gctttttata gaatagaact agtgttgttt tcatagactc
    23701 tcctttcttc atatttccaa ttaatcacaa attcacggca ttactagaaa aattaaaggg
    23761 gcgcagaagt ggaaaaatac tcttcgactt tccctacaaa ccaggccatc ttgtgtttaa
    23821 taactatacc aaatctaagg tacctctaaa tctaaattcc atttgtggcg gtgacctcca
    23881 atttttaact cttctatctc ccatccagta tacataacct tgatacttcg accacttata
    23941 tatcgaacac acaaatacct atccattgca ggagtactac ctaacttagt tgctacttgc
    24001 atctaaataa cagctgaaaa gataggactt actcttagta aagtatctga actattaatt
    24061 tttaaactat catttttatc ctcaactaca tgcacatctg ttgcaacttc actttctgtc
    24121 tcatccagat aaatgattaa ggaatcggga attgcaatat cttcagctga cgctggaata
    24181 gttgagctaa attagtcatc atatctttat tataaaataa ccaacagcaa aaaatacaat
    24241 gaaaaacaaa cttggaacta atgtatcatt aataaatgtt gaaaaattca gcaactcatt
    24301 actgtatgat tttagcagcc caaccaacat ataccctata aaagctaata agatggttag
    24361 gaatccttta gaatttttac tcattcatag acacttcctt aaataacttt tattatagaa
    24421 ggtattgtaa ataataatat tgacaaaaag tgactcaaaa taaaaaaaac cttacattat
    24481 actggaatga actgtattga tagtgtacct aaaagctcta ctgacataag atcgtgtaat
    24541 cggtaaagta attttattaa ttatacggga tgtaatcaat tagagaataa actaatcata
    24601 aaaggtactt ttacattcat aattgtcgct ttgagattct actttggttc actatctgac
    24661 ccaattgcac attttcgatc attacaagta ttggtgtcgt tagaacaact aaataaaaac
    24721 tagcggtaga tttttcagaa tctatgctaa tttctgtatg gatgttccag tgatggcaaa
    24781 tttctctttt cattgttttt cttgtcgcat gatatgcctt tttgtgttat tcagttaaac
    24841 tatagaaatt ctttcctaga cagatcttga tcccaaattt tacgtttttg agcatcgata
    24901 tagtttaaaa tgacattact catatctaaa gagtttagat tttttaggac taaaactgtc
    24961 agttgttctt tttcttcttt ctgttaaagt attgtcgcga cttttcgtat acgttaagat
    25021 atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa tgacttgagg aaatcacctc
    25081 aatcattcgt ttaaaatgct atatattctt tagtttaaga tacggaaatt aatgccatcg
    25141 accacactag tagaatgaac gacatttact tggccttttt ttaatttcgt tatgtttagc
    25201 atataaaaat attatagagt ttacttacaa tgcttaaaac atatactgta taatctgatc
    25261 aattttagcc atgtcttgca atggtaagtt ttctattttt ttgagttttc gttccttaaa
    25321 atcaagagat tttaattgtg taataattac ttgtcctttt atatctagct cttcaggtaa
    25381 tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt ggacatatga ccgaaaacat
    25441 tgttttacgg ttaaattcat aacgagagac gactagtcct ggtcgtcttt tttgaatttc
    25501 ttttcctacg gagggatcaa aatcaatcca tacaatatca ccttttttag gaatataaat
    25561 tctttcttct gacactataa aatttctctc ccttcaggag ttaaatcttc ccattcgtct
    25621 ttctcataat actcggcctc ttccccacca ctaaatggat cttcaatttt tggaactaac
    25681 gtaattgttc catcatcaga atacacaacg atatattctt ggttttctga aggtttcttt
    25741 ccgttatcag aaggtaaagt taccactaca gaacttcctt gtaatctaga ttttgtaaca
    25801 agcattatca tcactccctt aataaaagta tactatataa cactaagtaa cgcaaaattc
    25861 ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg gatataaata
    25921 gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca agaaacattg
    25981 aacctatgga tccaaaagct acccaaatac tatagctaac cccccgctac cttcaatttt
    26041 gcagataaaa cgctttggga gaaaatttca aagacaacag gccatcctat catgcccgat
    26101 tataaacaaa aagctggcta ttccttttgc tctgacaaat ttcaattggg aaagttcacg
    26161 aaactagcag gtgaagaagt ttcaactgtt cggatcaaag aatgcccgct tcaaatagaa
    26221 acattggttc aacgaatttc aactagacaa gactttgcta ttgtagaacg tgagatcgta
    26281 gctatttttg tagaagaagg gattttacat gatccaactc atatcaatgt ggataaatgg
    26341 caacctctta tctataaatt cagagaatat acaactgcca accagtcgtt aactttgaat
    26401 tttagattcc aagaattcaa agagtcataa tgataaacat cgttaggtat aaataaaaaa
    26461 gactcgcagc acgagtaaaa aacaaataga agctatacat tagaaaggtc gactgtgacc
    26521 ctatagatgc agtatatgca gcttatgagc atttcttctg ttctctttgt ttttgggatc
    26581 tgtgctgaag tctttttcgt tttttgccgc tctttctttg aaaaatcgat tatgttatac
    26641 cttctccatt actaattcag gcatctgctg agtaatgatt tttcgcgcaa aagaaatatc
    26701 tgttacccaa ggttcgattt gttcttcagt caagacaagc ggcattcggt catggattgg
    26761 tgcgactgtg cggttaggtt cagtagtcat tatgattgat tctgccattt tttcatcctg
    26821 atggctacga taaaaaccgc caatatacac tattttttcc ttatttgaaa acataaattt
    26881 gtctttttct tcattccact cattgggaaa acacaacgat tttgcaaaaa tggttgatga
    26941 aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg tcctttttta
    27001 aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc ctcttttctt
    27061 aacgtcacaa caagatttga cggaaaaact tctccagctg ctaattctgc tgcctgcttc
    27121 ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc tagatcaaaa
    27181 aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac actgcaacaa
    27241 gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg
    27301 acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt cacaatattc
    27361 aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg aaacggcagg
    27421 cttacgacct ctgggtaaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
    27481 tgcaaaaatg tagaaagcta ttttcacaaa atttttctta atactcgaaa ctaagcgact
    27541 ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    27601 ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt gatgcagggc
    27661 agctttttca caacctctcc actaaacggt gttcaatcag ctaaccttcg gtattagctc
    27721 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
    27781 gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc gaaaaataag
    27841 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    27901 tgaccacttc tgtcacaacc tctaattatt aaagaacctt ttgcgaagtt aacaatcttt
    27961 ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc ctgtgtgact ttcaacaata
    28021 agatacttcc ctggatttca gcaatcaacg ctttggcttg acggtctgct tcttcatcgg
    28081 acagtttttc ttcagaaagt gccaggatat tttgtttcgt atacttgacc cattgattga
    28141 ctaattctag tagcaacaga cggtattctt catccactgt actcaactca ataatcaaat
    28201 tacctacagg acagccatga taagtgatct gtgtctcatg atcagcgaaa atccagtcca
    28261 acatttcttc taatgcttct tctggctgtt ttccagcatt taagatgtct tcgaacaatt
    28321 ctgtctgcca tacgtccata tgcgcatgga tcaccgctag acaaacttct ttttttgaag
    28381 aaaagtaata ataaaattga ccttttcctg tatcagaagc tgctagaata tctcggattg
    28441 acgtttcatg atagcctttt ttataaataa gatctttggt cgcttccaag atcttttcct
    28501 ttgttttcaa tgatattctc ctttttcatt tctcatagtc ctatattata cagtaaattc
    28561 atcatttttt cttcttaatc gtttaaaaaa gactgtgcca aattatctga cacagccctc
    28621 actataaaac tgttctcata tagctagatg ttcattgatt caagaactgc gaggacaaaa
    28681 gcaattccta tcttatgata gttgctctaa tacttcctct gttgtcatca cttgatctgc
    28741 aataaagcca aagttcgtca acgctgcatc cattgcttct tggccaggag caccaattgc
    28801 atctgtcgct accacaacat tgaatccact ctctaccaat tcacgcatat gggaatcgct
    28861 acacaagttt ccgttcattc caccaagaat tactgtgtcg attccatttt ttctcaattg
    28921 gagcgccaaa tcattgcttt ctggtccaaa gattttatga ggagagctga tgatcgtatt
    28981 ttcatccaaa taaggtttca attcttcgat aaaatcggct cctgaacctt cttccactga
    29041 ttcatattga ctttttctag caaacatctg ctgctcaagc atcatatgtt ctcctgcacc
    29101 gccaaattgc catttatggt catgtgggta aaaataatga ggagaaataa agagtttgta
    29161 tcctttttct tttgcttgtt ttaccatttc aatcatattt tcgatcgtat tgtatttttc
    29221 taagatatct tttgtcaaac caaagccacc gcctgctggt ttcaagaact cgacttgcgg
    29281 gtcagtgaga actactgctg tatttttatt attgaacatg gtggattctc cattcgttat
    29341 ttattttaat acatcattta gagcttcact cattgtcgga tgagaataga tttgatctct
    29401 caataacgta taaggaagtt ttccattcat agctagcgaa atcaagttga tgacttcatg
    29461 agaatcttcc gcatagatac ttgcccctag aatcaagtca gtttcagggt cgactaaaac
    29521 tttgaataca cctttaggat cttctaatac ttgtgcttta gggacaccgc ttgtcatata
    29581 cttgaataat ttgtaattga cgccttgtgc tttggcttgt ttttctgtta aacctacatt
    29641 ggataaagtc ggagtgataa atacactgta aggcacagct ttccggttat tcgttgttcg
    29701 tttattctcg cccttcaact gatccataat gattcgataa tcatctagag agatataagt
    29761 gaactgcaat ccgcctttga cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag
    29821 gtattcatcc acagcgattg ctccgcgatc tgttagttta acatctgtat tttctaatcc
    29881 aagtgatgct gtacttggtt ttcttccagt tgcagccaaa acttttgacg cttggattgt
    29941 tacttcttgg ccatcttctg taaaggtgac ggcagctgtt gattcttggt ctgctacttg
    30001 atctacagat acacctagat ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat
    30061 ttgcgcgata tcttcatctt cacgtggaag aaattcagaa tgggcatcaa gaacgacaac
    30121 tttagatccg tattcattga acattgatgc aaattccaag ccaatatatc cagccccgat
    30181 gatcactaat ttttccggta attgttcctg atccatcgct tgcgtagaat caatcaagta
    30241 gcgacttgta ttcaatcctt tgattggtaa aatgattggt tcagaaccag tattgatgaa
    30301 aatccgttcg ccttcaacaa tcactttttc accattattc gtcacttcga tttgatggtt
    30361 agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg
    30421 ataatttttg gcgcgaagca tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa
    30481 gtcgattcct ctttctccat tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat
    30541 acatgttccg ccatacatac gtgttgactt ttcaacgact aaaaccgatt caccttttga
    30601 agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa ccgatcacaa tattttgata
    30661 tttttccatc atttttccct cgcttctttt ttacctaccg attggtacaa aaatatgata
    30721 accgataatt gtctattctc caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa
    30781 agcggtcctt tcacgttttt agtctggttc gatttccttt attcttctaa acaatcgtta
    30841 cttctacatc gatatttcct ctagttgctt tggagtaagg gcagaattca tgcgcatctt
    30901 ctgcaatttt ttgggcttct tcttcgctga tgccatcgat atgcacttct aatactacac
    30961 cgatctgata gccgccatca tctaaattat aaagagagac acgtgctttt actgtggact
    31021 ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc cccattgaaa caagaagcat
    31081 aacctgctgc aaataattgt tctggatttg ttccttctgc atggattcct ggaggagtga
    31141 ttttcatatg catcgagcca tttggtgcct cgacttctcc attgcgcccg ccatggttga
    31201 tcattgctgt ttcgtatgct tttttcattg tactgctcct ttcgattgac catttgctac
    31261 ttggctataa ttatagtgta tgacaagtaa ggcgctttct acaaaaattc tgcttattcg
    31321 aataccagaa tgccgtcttc tactattttt ccaaaaaaca aaaaaatact actcgtgaac
    31381 aacagcgtca tatttgttcc aaaataaata gcaatctttt ctacataatc ttttatatga
    31441 ttgattttaa gatacatata taccagaaag ttgaccccat aaaccaatac tgttaaaaaa
    31501 ataaccatat tcgttaccat tttttcctcc ttttgctggt ctccctacca gcactcgatt
    31561 taaccatacc actttttttg ccgtccaagc aactagcctt tcaaaaagac tggtttattc
    31621 tgctgttaat gattcaagat cagaaaaatg tccgaataat tttgctaatt ttactaacgc
    31681 caacttgttc tttccgaatt catcattttc tatctgtaat actagtaacg gctcatgtaa
    31741 gcttaaacgg atcaaaaacc agccgtttcc gtatggctct tctagatcat atcgaatacc
    31801 ttcttcattt tccgggtcga agttcatccc tttttcttct tccaacagtc gcttcatttc
    31861 ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg aacaatttta atcggatttc
    31921 ttgtacttct acgggctgtt tcaactcttt gatttgttca tctaatgtga taccttttct
    31981 ttttaattct ggcaaaagca tcaggatctt tgcgatgaca tatgcaccat catccagaaa
    32041 atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg gctagctgtg tatctttccc
    32101 aagacggtta agttctaatg ctttattgat tacatttcga tagccggaaa tataacgaac
    32161 ttggatgcct cctttttctt caatgaatct ttgtagatga gaagaagtag gggaattcgt
    32221 cacgattctt gttcctggat gctcttttaa tacgattgta cttaatactg cgatcaaatt
    32281 atttcgattg atcactgttc cagaagcact gacaactgca gaacgatcca catctgtatc
    32341 gaaaatgatc ccaagatctg cttcatttgc taaaacagcc tgcttgatac tttccatcgc
    32401 ttctttatta tcaggatttg ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg
    32461 ggaacctgtt gaatccgcac ctaagctatt caatactttt tcagcaaaga atccaccagc
    32521 accatttccg gcatcaacaa tgattttcca accatttagt ggtttgcttt ctagtgttcc
    32581 aatccccttt ttaatttttg tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt
    32641 ttctacgctt ccagtttgtt cgctttttat tttttgctgc tgtgatagga taaaagcaat
    32701 atcttctttt tctgctcctc cttctgaggt aaagaatttt attccattat aataataggg
    32761 aagatggcta gcagtcagca taaccgcagc atcgcacgag aactgttcaa attgagtgga
    32821 cataaacata gcaggcgtag tagctaattc gaaatcgatg acatgtatcc cttgttcttt
    32881 cagactttgg atcaacgcct ctttcagtgc tggtccgctt atcctactgt catgaccaat
    32941 cccaatcgtc cattttttat ttggatcggt tgatttcttc tttttcttta tccagtttgc
    33001 tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag tttacttcat atccttcaac
    33061 tggtaatgcg attcctcgaa tatccgatcc attttgcagc gctttcatat ctttcatata
    33121 aaagtcctcc tcatttattc cctcattttt tccagctttt ctgcaaatcg ctgtgcttct
    33181 tcttctgagc ggcaaataat aatgaatgta tctacaccgg ccatcgttcc gacaacttca
    33241 gggtaagcca cttcatctag ccaattcgtt accacatcgg caccatttcg ttcagtcaat
    33301 acgataacca tgaactgaac agactggata cgcaaaacct cgcgtctaat tgctgtacgc
    33361 aaacgttctt catttaaaga aacagatggc tggttaaata atgtatagcg tactattttg
    33421 ttttcatttt gactttttac taacttcatt tctcgaatgt ctcgagaaat cgttgcttga
    33481 gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct cttcctgtgt cccaataggt
    33541 tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt tctttttcat attagacacc
    33601 tctcaatgtt gattatagca aaagataagt aatatttctt catattttgg tattttttac
    33661 ttttattttt tgttttcatt caggttgcta acctttttct attttaacac gcaaaatcgt
    33721 cttcattttt tcaggtgctg attttcttgg atcgctgatg taaatctctt tgtgttcttt
    33781 agaagctctt tcatagcctt cttttgcgat gaatgcttct aatttctcaa acgttgctgg
    33841 ttcttcggca tagggcccga cgtgtaagat ttgggcacaa agaccttctt ccatcttttt
    33901 gaatactact tgttctttca agtcttccgg aagtttgcta gaacgctgaa tggcttcttg
    33961 tgctatcgct tctgtaacaa agtcaggctg tcggatcatc aattgataag aaaaatgttc
    34021 ttttttcatc actggttcat gcaaatactt ttcttgcagt ccccattgtc cttccagtgg
    34081 atagacagta tacatctgat aatcaggaat cagccaattt tttttaggcg acatgcgtat
    34141 tgcatagctg actgcatata gacaggctac tcttcgttta aagtcttcct gattcgggtc
    34201 gccgatccct ctgatgctaa agaatgattt tttaggaacc tctagtattt gcgggctttt
    34261 tttcatacta tagctcttgt ctttacgcca ttcaattttt tccatttttc ttcctccgcg
    34321 aaattccatt tagttattta ttatcatacc tctttttgag tgacttggct aatcgtgttg
    34381 acactatttt tcagtttgtg ctagaataag tgaagtaaat aaatcgtaca gataacacgt
    34441 ttcggcgtgt cgagagagag gcggtttcta tttttataat agaaatcact ctcttttttt
    34501 acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc
    34561 agtaattgca acagggatga tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt
    34621 aactttccca actcttatca gacagtttga tttaacgact gcacaagtcc aatgggtaac
    34681 aacgatttat cttttgatga tttcgatcat tgttccgctg tctacttatt tgaatcgcaa
    34741 ttttagtctc aaaaaactct ttttagcttc aaatctgctt ttcttggcag gtataatgat
    34801 cgatttcttc tctcctacct tcatggtcct attgtttggt cgattgctcc aaggtgcagc
    34861 gacaggtatc gctcttccgt tgatgttcca tattatcttg acatttgccc ccctcaatcg
    34921 acgaggcgcg atgatgggtg tcggtacttt gacaacggcc attgctccag caattggccc
    34981 cacttatgga ggcattatga cttcacattt tacatggaat catatcttcc tgtttcttgt
    35041 gcccgttttg tttctttctt tgattatcgg ctggtttgct attcccgaga tggcagtaca
    35101 aaaaagtggt tatttggatt ggataagcgt tttaggaatt tgtcttttgt ttagtggaag
    35161 tctaacattt ttgagtacgt tgggaacagt ttccagctgg attgctttaa tcatcggcgg
    35221 tattggctgg ttcctttttt actatcgaag taaaaaagtg gagcatccac tgatccgact
    35281 gtctattctt tcaaataaaa cattccgctt gtttttgatt ggcttcttag tttatcagtt
    35341 tttattactt ggtgtatctt ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag
    35401 tccttttgtc gctggattag cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc
    35461 tttttccggg aaaatgctgg atcaatatgg tccgaagaag ccaatttttg ctgggctgat
    35521 tctttctttg attggttggc tagcattatt cctactcata ggacacgctt cagtgggtgt
    35581 attgattgct tgtcatgtgt tttatatgat cggtatcgga ttatcttaca gcaatatgat
    35641 gaccaccgga atgaatgcac tgaatgaaga actacaagga gacgggaacg caatgttcaa
    35701 taccttgcag cagttttctg gcgctgtggc tacttcatta gtagcggtca ttatcaatta
    35761 cgtccagcat catacttccc ataactatga agtgtctact acattaggat caaaagcagc
    35821 attaggcgtc ttgttgctgt tgcttctcgt aagctttgct cgattcgcct attatctgtt
    35881 ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt gcatctgccg tttttttgtg
    35941 tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa aaattgccat tatcggtggc
    36001 gggatcattg gtatgaccct agccagttac ttagatacat caaaattcaa tgttgcttta
    36061 tttgatgaag ggacaggaca ggcaactaaa gctagcgctg ggattatctc cccttggttg
    36121 tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag acggcgctgc ttttttccct
    36181 aaacttataa aagatttcca acttgatcaa acgatttacg cacaatcagg gacacttttg
    36241 cttcgacctg aaaaagattt agctgaattg gctgttcttg ccgagaaacg aaaagaagaa
    36301 gcgccagaaa ttggaaacat cgaatggctt tcacctgaac agacatctca agcacttccg
    36361 ttattgaaac cagcagtttc actgcgtatc agtggtggtg gtcgtctgga tggaaaagca
    36421 tatttagaac atcttggtca gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa
    36481 gtcactttgc ataagaagag cgattcttgg ctcatcaagg gaccgtttgg agaatacaca
    36541 gctgatcgtg taattttgac acctggcccg gcacttaagc ctttattaca agaattaggc
    36601 tatgctgcag atatacgtcc gcaaaaaggg cagctcttag catttcagac atcttataag
    36661 atgagtcaag actggcctgt tgctatgctt gacggagaag ctgatttcat tcctttcatg
    36721 gatggaaaga tccttttagg tgctacccat gaaaacgatc aaggctggga tttggctcct
    36781 actagcgaag cttataaggg attgactaag agtacagaac agttcttgac tgatccagat
    36841 cagataaaaa actggccata cgagttccgt gtaggtaccc gtgcttatac gtcagatttt
    36901 tccccatttt tcggcgtact gccagatgat ccaagcttac tggctgctag cggtctaggc
    36961 tcttctggct tgaccacagg tccttatatc ggctatctat tagccaactt cttaaatact
    37021 ggtcattggc ctacggaaga ttatcaaaag gacatctcca cctatattac gaaaaatcca
    37081 tcatagaaaa acagcttttt caagtaagga tcccgagcga tattgcgcat ggattacttc
    37141 ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat tctccaaact ttcttttgct
    37201 tcatctggca agagatcttt tcctatctga gcttcaatga atcgtaaacg aatatcaagt
    37261 cgttgtttca ccagattttt ataggacgta tcctcaaaag aaacaggaaa atctggtata
    37321 ggtaaatagt ggatgtcttc aaatggacca aacgacttga attctgccgt ttgttgcacg
    37381 attctttcaa cgattgacaa tgtcgtttca ggtttgattt ttttatcatc gaaatatcca
    37441 gtattcagga catagcaggc tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag
    37501 tcttccctaa gaggatagac acggaaagga ttagcaaatg gttcaatgac tagctcatct
    37561 agctgtcttt ttgtagtgat attttccgcg tctgatcgtt ttgttgctaa tgttgcacca
    37621 aaagacgctg ctaaaactgg gtcattgatc ttgataattg gcggaagcga atcatctttc
    37681 atgatccaaa aaacagcatc gatcttttcg ctaagatgat cgactcggtc gggtgtcgca
    37741 taacgtgatt tgactgtccg cccatttccg tttcgtatat ctcctgttac taggactttt
    37801 tctcctttac ttgtacatgt cacgcccaca ttctgacaag taaggaaaga agaaatggcc
    37861 ggatcatcag acggatagtc ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca
    37921 aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca agatcgaaac agagtgacct
    37981 tccgtatgtt ttgctaaggt aatcgttgat tttccagaac cggacaaccc aaatacagcc
    38041 atcgtatagg tctggtcatt actagcatat tgtttcatcc caccgtggca ggcaataaag
    38101 ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag
    38161 taacgtaatc ctagcacagc cgctgtatta tggagagcat caaacaaaat gagtccattt
    38221 ggaaatgttt catgtgtcca ttcgggatca caaaagatat aaagatctcc ctcatcaaag
    38281 gaaacagact tttgataagt ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa
    38341 taagaataca aattgttctc aaatccttct ggaatcagca aatgactttg cacagaaaat
    38401 tcttcagata ggccaacgat tacttttgta tggtaaaact ggcgcttgcg actttgatac
    38461 acagcttctc ttaaaatttt ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc
    38521 cgccgagcac ttgctgttct tcccacaatg cttccatcat tgaatactag tgtcctaaca
    38581 tcagctggta aatctagttc ttcagcatgt gagattgtta aatctgtgat gatcgttcct
    38641 ggactccctt ttgctagttg ataagctgtc ttcctatcaa aaacttcatg aacattatta
    38701 ccgtaaaatg tcgtttctac aatcgatcga atactggata acagtggatt tccacttgtg
    38761 atttcgttta attcgagact ctcttttgta ctcatggctc ctcctcattc cctaaacaaa
    38821 aaaacgtttt ctttattata cattattcga gtttttattt gtactgctgc gctgtgggaa
    38881 gcaaacgagt tatttttcac acaaaaaaac cgagaacttg aataaacaag tttctcggtt
    38941 tcatcatttt ccttttatcg gcttagtacc agccgtttgc taaccagaat gttttagcag
    39001 catcccatga accataacga gatgttacat attgttctgc tacacgttct tgattggcag
    39061 ctgaatagtc gccgtttaag tatgaagcat ctaattgata acggccgatg taacgtccgt
    39121 tagtagctgt gtatgaaccg cctgattctt tttgtgcgat ccattctttt gcagagcttg
    39181 aagttggcgc tgttgcagca gctggggcag caggagctac ttcaactgtt tcaacaggtg
    39241 caacgtatgt atgttcttgc gttgctgttt cagttgtaga tgctgtattt tctgttgttt
    39301 tgttttctgt tgttgctgaa gaatcatttg aagttgggat tgttaattgt tcaccaacat
    39361 aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat tgcatcgatc attgagttgt
    39421 ctcctgcaaa tttttgtgag atcttagata atgaatcacc tgattttact gtataaactt
    39481 catcagcgtg agcatttgta gttcctacaa acattgcagt agcaccagca gcaaaagttg
    39541 tacctaaaac aattttctta aatgaattca tattaaactc ctttttttct ccgtttttta
    39601 ttctttcgac g
//
LOCUS       DAZUMO010000023        37991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000023 DAZUMO010000000
VERSION     DAZUMO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.023"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..877
                     /locus_tag="ACG8ER_001170"
     CDS             8..877
                     /locus_tag="ACG8ER_001170"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS5049766.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1042..2313
                     /locus_tag="ACG8ER_001171"
     CDS             1042..2313
                     /locus_tag="ACG8ER_001171"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5049767.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2310..3605
                     /gene="rho"
                     /locus_tag="ACG8ER_001172"
     CDS             2310..3605
                     /gene="rho"
                     /locus_tag="ACG8ER_001172"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS5049768.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3704..3967
                     /locus_tag="ACG8ER_001173"
     CDS             3704..3967
                     /locus_tag="ACG8ER_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS5049769.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4412..4861
                     /locus_tag="ACG8ER_001174"
     CDS             4412..4861
                     /locus_tag="ACG8ER_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5049770.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4918..6336
                     /locus_tag="ACG8ER_001175"
     CDS             4918..6336
                     /locus_tag="ACG8ER_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5049771.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6336..7196
                     /locus_tag="ACG8ER_001176"
     CDS             6336..7196
                     /locus_tag="ACG8ER_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5049772.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7370..8143
                     /locus_tag="ACG8ER_001177"
     CDS             7370..8143
                     /locus_tag="ACG8ER_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5049773.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8290..8655
                     /locus_tag="ACG8ER_001178"
     CDS             8290..8655
                     /locus_tag="ACG8ER_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS5049774.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8803..10011)
                     /locus_tag="ACG8ER_001179"
     CDS             complement(8803..10011)
                     /locus_tag="ACG8ER_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5049775.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10030..10308)
                     /locus_tag="ACG8ER_001180"
     CDS             complement(10030..10308)
                     /locus_tag="ACG8ER_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049776.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10501..11157
                     /locus_tag="ACG8ER_001181"
     CDS             10501..11157
                     /locus_tag="ACG8ER_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5049777.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11599..12396)
                     /locus_tag="ACG8ER_001182"
     CDS             complement(11599..12396)
                     /locus_tag="ACG8ER_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS5049778.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12393..13577)
                     /locus_tag="ACG8ER_001183"
     CDS             complement(12393..13577)
                     /locus_tag="ACG8ER_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS5049779.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13602..14849)
                     /locus_tag="ACG8ER_001184"
     CDS             complement(13602..14849)
                     /locus_tag="ACG8ER_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5049780.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15064..16809
                     /gene="dnaX"
                     /locus_tag="ACG8ER_001185"
     CDS             15064..16809
                     /gene="dnaX"
                     /locus_tag="ACG8ER_001185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS5049781.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16829..17143
                     /locus_tag="ACG8ER_001186"
     CDS             16829..17143
                     /locus_tag="ACG8ER_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS5049782.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17168..17764
                     /gene="recR"
                     /locus_tag="ACG8ER_001187"
     CDS             17168..17764
                     /gene="recR"
                     /locus_tag="ACG8ER_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS5049783.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17828..18472
                     /gene="tmk"
                     /locus_tag="ACG8ER_001188"
     CDS             17828..18472
                     /gene="tmk"
                     /locus_tag="ACG8ER_001188"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS5049784.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18487..18816
                     /locus_tag="ACG8ER_001189"
     CDS             18487..18816
                     /locus_tag="ACG8ER_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS5049785.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18830..19768
                     /gene="holB"
                     /locus_tag="ACG8ER_001190"
     CDS             18830..19768
                     /gene="holB"
                     /locus_tag="ACG8ER_001190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS5049786.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19804..20628
                     /locus_tag="ACG8ER_001191"
     CDS             19804..20628
                     /locus_tag="ACG8ER_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS5049787.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20621..20968
                     /locus_tag="ACG8ER_001192"
     CDS             20621..20968
                     /locus_tag="ACG8ER_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS5049788.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21037..21909
                     /gene="rsmI"
                     /locus_tag="ACG8ER_001193"
     CDS             21037..21909
                     /gene="rsmI"
                     /locus_tag="ACG8ER_001193"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS5049789.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22018..23139
                     /gene="dinB"
                     /locus_tag="ACG8ER_001194"
     CDS             22018..23139
                     /gene="dinB"
                     /locus_tag="ACG8ER_001194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS5049790.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23193..23795)
                     /gene="nrdG"
                     /locus_tag="ACG8ER_001195"
     CDS             complement(23193..23795)
                     /gene="nrdG"
                     /locus_tag="ACG8ER_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS5049791.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24110..26299)
                     /gene="nrdD"
                     /locus_tag="ACG8ER_001196"
     CDS             complement(24110..26299)
                     /gene="nrdD"
                     /locus_tag="ACG8ER_001196"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS5049792.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26625..27392
                     /locus_tag="ACG8ER_001197"
     CDS             26625..27392
                     /locus_tag="ACG8ER_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5049793.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27382..29424
                     /locus_tag="ACG8ER_001198"
     CDS             27382..29424
                     /locus_tag="ACG8ER_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049794.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLITGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            29705..29854
                     /locus_tag="ACG8ER_001199"
     CDS             29705..29854
                     /locus_tag="ACG8ER_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS5049795.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            29865..31379
                     /gene="dltA"
                     /locus_tag="ACG8ER_001200"
     CDS             29865..31379
                     /gene="dltA"
                     /locus_tag="ACG8ER_001200"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS5049796.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            31376..32581
                     /gene="dltB"
                     /locus_tag="ACG8ER_001201"
     CDS             31376..32581
                     /gene="dltB"
                     /locus_tag="ACG8ER_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS5049797.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            32626..32859
                     /gene="dltC"
                     /locus_tag="ACG8ER_001202"
     CDS             32626..32859
                     /gene="dltC"
                     /locus_tag="ACG8ER_001202"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS5049798.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            32862..34130
                     /gene="dltD"
                     /locus_tag="ACG8ER_001203"
     CDS             32862..34130
                     /gene="dltD"
                     /locus_tag="ACG8ER_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS5049799.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            34271..35353
                     /locus_tag="ACG8ER_001204"
     CDS             34271..35353
                     /locus_tag="ACG8ER_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS5049800.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            35353..36156
                     /locus_tag="ACG8ER_001205"
     CDS             35353..36156
                     /locus_tag="ACG8ER_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5049801.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            36226..36966
                     /locus_tag="ACG8ER_001206"
     CDS             36226..36966
                     /locus_tag="ACG8ER_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5049802.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            36977..37981
                     /locus_tag="ACG8ER_001207"
     CDS             36977..37981
                     /locus_tag="ACG8ER_001207"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS5049803.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        12187 a         6706 c         7919 g        11179 t
ORIGIN      
        1 ttttattatg ccagtagtat caggagctga atttttaaaa gctgctcgta aaggcggtta
       61 cgctgtcggc ggattcaaca caaacaactt ggaatggact caagcaatcc ttgaagcggc
      121 tgaagcaaaa caagcaccag tacttatcca aacatcaatg ggtgcagcta aatacatggg
      181 tggttacaaa atctgtaaag atttaatcac tgatttagtt gaagcaatga acatcactgt
      241 tccagttgct atccacttag accatggtga ttacgaagct gctttggaat gtatcgaatt
      301 aggctatact tcagtaatgt tcgatggttc acatctacca tttgaagaaa acttgaaatt
      361 agctaaagac gttgttgaaa aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg
      421 ttcaatcggc ggagaagaag acggaatcat cggtaacggc gaattagctg atattgaaga
      481 atgcaaacaa atggttgcta caggtatcga ctacttagct tgcggtatcg gtaacatcca
      541 tggtcaatat ccagaaaact ggaaaggatt agcttttgac cacttacaag caattgctga
      601 tgctgtaggt ccagacatgc ctttagtatt acatggcgga tcaggtattc ctcaagatca
      661 aatcgaaaaa gctatctcaa tgggtatctc aaaagtaaat gttaacactg aattccaatt
      721 atcatttgct gcagctactc gtaaatatat cgaagctggt aaagataaag aaggtaaagg
      781 attcgaccct cgtaaattat tagctccagg taaagcagca atcgttgaag atgctaaatc
      841 tcatatcgac tggttcggtt cagctaacaa agcgtaagca tccacataac agatacgata
      901 tataggaatg gagaacgact tttgaagtcg ctctccattc ttttttactt aaaacggcag
      961 accatgctcg tttctttttt ttatctatga taaaatacaa gcatatccat gaaaaaagat
     1021 taataaacaa ggtggaaaca gatgaaaaaa atcgtaatca aaggaaaccg acctttagct
     1081 ggtgaagtaa cgatcagcgg tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc
     1141 ttagctgatt ccccagtgat cttggaaggt gtgccggaca tacaagatgt tcattcatta
     1201 attgaaattt tagagatcat gggcgcaact gtccattttt ccaacaacat actagaaatc
     1261 gatcctcgag aagtcgtatc gattccgatg ccgcacggaa aaatcaatag tttgcgtgct
     1321 tcttattatt ttatgggaac actgcttggt aaatttggcg aagcggtagt aggcctgcca
     1381 ggtggctgtt atttaggccc tagaccgatt gacctacacg tcaaaggatt tgaagctcta
     1441 ggtgctgaag tgacaaatga acatggtgcc atgtatttac ggacagaaaa taaaggattg
     1501 cgtggcaatc ggattttcat ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta
     1561 gctgctgtaa aagcaaaagg acagacagta attgaaaacg ctgcacgaga accggaaatc
     1621 atagatgtgg caactttact gaataatatg ggggcaaaag ttcgaggagc gggtacagat
     1681 gtgatccgta tcgaaggagt cgaaacatta catggctgtc ggcatttcat gattcctgac
     1741 cgcatcgaag caggaaccta tctagcatta gcagctgcag taggaaatgg tatcaaagtg
     1801 aaaaatgtga tctttgaaca tttggaaagc tttatagcaa aattgcagga aattggtgtc
     1861 catatgaaaa tcagcgaaga tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca
     1921 aacatcatga cctatcctta tccaggattt gccacagatc tgcagcagcc acttactgcg
     1981 ttactattga tgactacagg cacttcagaa atcatcgata cgatctatgc gaaacgtgtg
     2041 aaccatgtgc cggaactagc acgaatgggc gctgatatta ccgtggaagg aaatatgatc
     2101 atcgtgaatg gtccaaataa acttcacggc acagaagtcg tggcttctga tcttcgggca
     2161 ggtgcttgtt tagtgattgc tggactattg gcagaaggga caacgacgat ttacaatgta
     2221 gattatatct tacgcggtta tgatcatatt atcgagaaac taacagcctt aggggcatca
     2281 attgaaatga tagaggaagt gggagctgaa tgagcgatta tttgacaatg gcagaactag
     2341 agaacaaaac gttaaaagaa gtttatagtt atgccaaaga atttaaaatt ccttattaca
     2401 gtaagatgaa taaaaaggaa ttatctctag ctgtgattcg tgcacaagca gagaaacaag
     2461 gattttatta tatggaagga atattggata tcgttggtca agatggttac ggttttttac
     2521 ggccaatcaa ttatggacca agtgcagaag atatctatat ttctgcctca caaatcagac
     2581 gattcagttt gagaaatggg gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg
     2641 aacgttatta tggtttgatg cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa
     2701 aagaacgtcc gcattttcca gccttaacac ctctttatcc gcaaagacag cttcaactgg
     2761 aaacaacacc gcaaagacta tctacccgaa tgatcgatat cttttctcca atcggttttg
     2821 gtcagcgtgg attgatcgtc gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa
     2881 tcgcaaatgg tatcaccgaa aactatccgg aagtagaatt aatcgttcta ctgattgatg
     2941 aacgaccgga agaagtaacg gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta
     3001 catttgatct gcagccgcaa aaccatactc gagtttctga attagtattg gaacgcgcaa
     3061 tgcgtttagt cgaagataaa cgagatgtcg ttatcttgat ggatagtatt actagacttg
     3121 ctcgggccta taacttagtc gttccaccaa gcggacgtac attgagtggc gggattgacc
     3181 cagccgcatt gtataaacct aagaaatttt tcggggcagc aagaaacatc gaagaaggtg
     3241 gcagtttgac gattttagcg accgctttag tcgatacagg aagccgtatg gatgacgtga
     3301 tctatgaaga attcaaagga actggaaatc tagaattaca actttcacgt gaattatctg
     3361 aacgtcggat cttcccagcc atcgatatca aaaaatcagg tacccgaaaa gaagaattat
     3421 tgatgccaag tgaagaatta gaagaaattt ggaaacttcg aaaaaatatg actggagatt
     3481 cattagaata tacggaacaa ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat
     3541 ttttcaaaga atttcaagat gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa
     3601 gataattgca accttgactg aaacatgtta agatgttaat gttgtaaatc gacaaatcaa
     3661 ctctggttca gcaaatggag cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca
     3721 tccagattac cacccagtag tattcatgga ttcaacaact ggtttcaaat tcttgtcagg
     3781 ttcaacaaaa acttcacaag aaacagttga atgggaagac ggtaatacat acccagtcat
     3841 ccgtgtcgaa gtaacttctg actcacatcc attctacact ggacgtcaaa aattcacaca
     3901 agcagacgga cgtgtggacc gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc
     3961 ggaataataa aaacgctgat aactcaacgt ttagcagact atctgatagg gatagtctgt
     4021 ttttttatgt tttttgccaa tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat
     4081 aatgcacaaa acaatgaaaa actagtatat agttttaatt atccacttga taaaaacacc
     4141 ttatattaac gaaatgaaga ggggttattt cgaagtaaaa aaccatttac ctaaaattag
     4201 aagctaagct ttcctttcaa ggaaggcttt ttgttttgtt atagacttgc gattgtttgt
     4261 gagctagatc gttcatttta atgacttata cacgcggatg atgggtcttt ttcctctgtt
     4321 cattcataat attgaacggt acagctagcg atgctatact gacctcagtt ccaagaaaac
     4381 gttaacattt tttgtggggg agaggttttt tatgaggttt gatcaattga tcgcagaaga
     4441 actgattgtt ttcgatccat cgatagattc aaaaggatca ttatttgagc gaatagggga
     4501 tgtgttgaaa aaaacaaata aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa
     4561 acgagaggag gagatctcca caggaataga agatggattt gggattcccc atgcaaaaag
     4621 caaatatgtg acaaaaccag cactgtgttt ttttcataca gccaaattaa cggactatag
     4681 cgggatagat gaaatgccaa ttgaatatat ttttgccatc gtagttcctg aaaaatcagc
     4741 agatatgcat ctagagattc tcagcacact ctccagacga ttgatgaatg cagattttag
     4801 aaagggaata aaagaagcca ctacgccagc agatatcatc agacagatta gtcaagaatg
     4861 aaaatagtag agtctttgtc cgagtatcga tagtgaagaa aaaggaggaa aataatcatg
     4921 aaaatcgttg gtgtaactgc ttgcccgact ggaattgctc atacttatat gtcggcagaa
     4981 aaattagaag taacagcaag aaatctaaac catcaagcaa aattcgaaac acaaggtgta
     5041 aaaacagaaa atcagttatc tgaacaagaa atcaaagagg cagatgcaat cattcttgca
     5101 gtagataaag aaatcgaatt ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca
     5161 agccgagcaa tcaaagaacc gcaagtagta atagacgaag cattgagaga tattggcgta
     5221 ttcgttgtgt ctaatgaaaa agaaccagct gcaaatgaga aaaagccgac aatctataat
     5281 catttcatga atggtgtgaa ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc
     5341 gcactgagtt ttgcctttgg gattacagcg gctgatccca actcagcaga ctataatgtg
     5401 ttagcagctg cattttcaag gatcggagga gatacagcgt ttgccatgat ggttcctgcg
     5461 ttaggcgcgg ggattgctac atcgattgct ggaaaagcag gttttgcccc aggaattgtt
     5521 gccggattac tggcctctac aggtggttca ggttttcttg gcggaatgat tggtggcgtg
     5581 ctagcagggt atatctgtga ttttctggca aataaaatta gcgtaaaaaa agaattttct
     5641 gctatttatc agctgattgt cgtccccttt ttatcgatct taattattgg gcttttgatg
     5701 gtatttgtga ttgaacaacc gatcacatgg gtgttggatg ctttgacaaa ttggctgaat
     5761 agcttgggaa atacatccgg gctattgttc ggattactta tcgggatcat gatggcagct
     5821 gatatgggtg ggccgatcaa taaatcgact tcgactttct cgatcggttt gatgtcagct
     5881 ggagtaaatg caccgattgc tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt
     5941 gctttagcca ctttattatt caaacataaa tttaccaaag aagaacggac ttcaggtcaa
     6001 tcatgctgga ttttaggcgc ttcgtatatt acagaaggtg ctataccgtt tgcggtatct
     6061 gacccgatcc gcgtgatacc tagtttgatg acaggttcag cagtcgctgc agggatttcc
     6121 atggcagtag gagttacttc aatggcacct catggaggga tttgggtgat gctaattcca
     6181 aatgttatga acaatttacc tatgtatgtc ttggcaattg catcaggtac agtcactaca
     6241 gcctttatgc ttggactatt gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg
     6301 aaaaatgaaa atctaacaaa tgaggtggaa gcgtaatgtt atatacaatg aaagagttat
     6361 tgaaagtggc taaagagaat caatttgcag taccagcctt caatatttgc agctatgata
     6421 tgatgaaagc aatcatcgaa gaagcagaga gattagaagc accagtaatc ttagaaattc
     6481 atcctgatga aatcgcctat ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt
     6541 atacaagtaa actgccgatc gttatccact tggatcatgg cggtacgata gaagatgtct
     6601 tacgggcgat tcgaaacggc tattcttccg tgatgatcga tgcgtctttg aaatcattcg
     6661 acgacaatgt acagattacg aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag
     6721 tggaggctga gcttggaacg attgggaata atggttctgc tgaaggaggt gctgaaacaa
     6781 tcatttatac agatcctgat caagcgaaga tttttgtgaa agaaacagga atcgatacac
     6841 ttgctgtagc aattggaaca gcgcatggct tgtatccgaa aaacagaact ccaaaattaa
     6901 atatcgactt actgaaagaa ttgaaccaac aaatcgatct tccatttgtt ttacatggcg
     6961 gttcaggtaa tccggacgca gaagtcagcg aagcagtaaa atacggagtt gccaaagtga
     7021 atttgagctc agatcttaaa agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa
     7081 atccatcgat gtacgagccg aatgcaattt atccagcagc taataaaaag gtacaagaag
     7141 tggtcaaaca taaaatggaa attttgaaca cagtaggcaa ggctagttta tactaaaaat
     7201 aaaaatgtgt ggtcaagagg gtatgacaat agtcttgtca taccctctta accacacacg
     7261 atgttcagaa gtagtttttt cgaaaaattt cttcttattt ctcgaagcca atcacttctg
     7321 tcacaacctc ttttttcctg ttactatata aagtaaatgg ggtgatttta tgttgaatga
     7381 agtggagaat agacagcagg agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt
     7441 caccgatttg atcgagttag taaattatag cgaagccacg attaaacgtg accttgtttt
     7501 tctggaaaaa agtggattga tccgtagagt tcgcggtggc gccatgctta ttgacaatcg
     7561 aaaaattgac gtgccttacc tgatgaagat caccaaatta gaagaagaaa cagaaaaacg
     7621 taagattgca gatacagctt cttctttgat tcgagatgat atggtcttat tcttggattc
     7681 cagtacaacc tctttacact tagtcagaaa cctagcaaga tttgatggtt tgcagatcat
     7741 tacgaatggc atcgtcacag ctgctatgtt gagtgaatat acaacggcaa aagtatctgt
     7801 attgggaggg gcaattgttc cgaaacgagc aacaatcaac ggttcaaaag cctataacga
     7861 tgcattgtca tattatgcgg atttagcgat aatgagttgt cgcgggctag atttcagcca
     7921 aggagtaaca gaaactcatg aaggagaggc aatggtcaaa aaagctttta gaaaacaatc
     7981 agaccggctt gccgttttag ttacacagca aaaaattgca cataaattca tgcatcaagc
     8041 cattgctttt catgaaatcg attatctggt gacaagtaaa gaactgacaa aagaacagta
     8101 tgcgaaagta aaggaacacc atatcgaatg tctgtacgga taaattatta cgtgaatgtt
     8161 ctaagtggct tcctcatttt tagataggac cttatctttg ctcgcttctc tcagccgatg
     8221 gtataataat gaaaaggtta tcttgttaga taatcttact tttttatcca ggagaaagga
     8281 gtgcagaaaa tgaaacatgt gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt
     8341 ctgtgggtcg tgctgacagg tttatacggt gttagcttta tggattcaac gattgttggg
     8401 gtaattattg tagtcatgat ctatgtgcta ggggatctgt tgatcttacg taaagtaggg
     8461 aacatcgcag cgacgatcgc agatgctggt tcagctgcag ttatcttgtg gctctatcta
     8521 tattttatga atgatacagt cgatatttgg atgaaagtat tgattcctgc tctattgatc
     8581 ggtgtggcag aatggttctt ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa
     8641 cggaaaatgg aataaatcaa cagttaaact attcttatcg ataaatgaaa gctttaaaaa
     8701 taaatagatg tttaattaag aataatcata aactattttt actcaaaaaa ccgctcagat
     8761 tattttcaga tctgaacggt tttttttatc tattcatcaa gatcaaattt catttttcaa
     8821 tgattcaatc aattcgccct ctgcttcatg actatgtttt cggccattaa caagatagag
     8881 tgctgtacat atcaatggaa tgactccggc aatcacatac aatcctctaa atgaagtaag
     8941 agattttagc atccccatca aataaggacc aacgcctaag cctaggtcta aaccaataaa
     9001 gtaggtagat aaagcaatac tgattcgatg ttcgcggacg atttttaaac aaacagcctg
     9061 tccatttgac ataaacgtac cataacctag gccgactaat gctccagaga taagcaatgt
     9121 cacactattc gtcgttacac tcagtaaaaa taatcctgct gttaagaata aatagctagg
     9181 atacatcacg tattgttctc ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa
     9241 tgtgatgacc aaagcatata cgacgaagaa aaatgaccca gcagcaacta agtggattac
     9301 ttttgtataa gaagataaaa atgataaaac acttgaataa gaaagaccca tcaaaaaggc
     9361 aattcctgta atgaaaagtg ctttcttttc aataaaacta ttgatattcc atgagcgaag
     9421 agatattttt tgttcttcag ataatggaat attttttaca ggaaagacaa ggcaggcaat
     9481 cgtaacaaag aagaccagta caattgaaaa ccagataatg aaacgaaaat cagtcatatt
     9541 cagcaaaatc attcctaaaa atggcccaac agcggcagct aaactagtac ttaatccata
     9601 ataattgatg ccttcccctt ttttagatgc agggatatat gcagtaacga tagcatttgt
     9661 agctgtagaa actgtaccgt agccgaatcc attcaagaat cgaactaaat acaatgcccc
     9721 gatagttggc gtataaagat atgctaatgt tgaaaggaga tagaagatcg cgccgcctct
     9781 taaggtaaac ttccgaccaa atagttctaa ctgtttcccc atgaataagc gtgccagcaa
     9841 tgttccgatg atatagatcc cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt
     9901 gtcttgcgca atgactgcca tgatgaccat caacagataa taaatcaggt agacaataaa
     9961 attgatcaat gtgattccaa taaaatattt attaaataat ttcttttcca tgtatgttat
    10021 acattcccct taagctgaat atgcttttaa tttttgtatt tgaacttcta catcgtgata
    10081 attggatgga tagcgatcaa caagttggcg aataaataaa tctcttgtag acataaacag
    10141 atttaaaaat tcttcgcatt cgtttgttgg attctcatcc actgctttga tcaattcgat
    10201 cgagttttga tacgcatcat ttaaaaagac taaagaagct ttcatatcaa atcttgtttg
    10261 atatgctgtt tctaatcgat taatgattgt gttcatccag taattcatgt tttttccctc
    10321 tattcttaaa aaattttact tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat
    10381 aagtgatgga aaaaggtact taacaaatgt taatatcatt agtaaaatac ctttggactt
    10441 taaaaagttt taaagtactg tatgatcttt ttagatgaaa gtttggatgg gggagcatca
    10501 ttggatagta gagtcttaaa ggaatatttg gataaaaatg attttccgat tatacgcaaa
    10561 ggatatcata aatatttgac attcgaaggg atggaagatt cccatacgta tatcttgaaa
    10621 gagggaacgg taaaagcaag tgttatttca aatgatggga gagaatttaa ccttcgctat
    10681 atcaaagatc ttgaaatcgt ctctctttta aaagacgaat attcacaatt catcgattcg
    10741 ccgtataata ttcgagttga atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc
    10801 ttttggcaaa atatcaacca agacccaaag ctacaacact atgtaaaaga gtattaccgc
    10861 tatcgtctgc tttattcgat gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga
    10921 gctgtttgta cgcaacttta cgaattatat gatttattcg gtgtgcggac agaagaaggg
    10981 atgctgatcg atttttcgat cacgaatgaa gaaatcgcca aattttgtgg catctcaact
    11041 gctagcagtg taaacagaat tttaaaacaa ctgaaaaaag atggtattat caccgttaaa
    11101 gaccatcata tcgttatttt gaatatggac ttgttagaag acaatattat cttttgatga
    11161 acaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggaaaaata
    11221 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
    11281 taccaagagg ttgtgagcct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac
    11341 agcttttcat gtttgatgaa tattgtgact tgtgaccgag gagttggtag gagaacaccg
    11401 tttaccaaga ggttgtgaaa aagctgttct gcatcaagta ataagaagag ccaagtaaaa
    11461 acagtctctt atgtttttaa cttggctgga cttatcaccg tagatgtaag cttttgaacg
    11521 ccgtttattt acaacaaaag gggctgtgac acgatttttg tcacagccct ttttttgcga
    11581 acattcattt agcaattttt agaaacttct gccgccaccg ccaaaagtcc cgccaccgct
    11641 agaatgggta gtacttccgc caccgccgct tccaggaggt gggcttttgg ggattcttct
    11701 tgtcgtgaca aaagaatttg tcagacgatc ggttttttca gttaatttga tactggattt
    11761 ttctcggaat ggatatttgt agatgcctga tttcaattga taacgcgaaa cagtcacaaa
    11821 ataaaagaca agactaagga taagagccaa aacaacagca atgatgattt ctactgtcgt
    11881 caaaacctta tagcgtgtaa ttttgccagt ttcttcgtct acacgataat gtcctcctgg
    11941 aactccgtct tccacatatg ttgctgattt agacaggtaa gcttttgcag cattgtaata
    12001 ggcagcattt gtcatctgat cataaacatc atctaagatc gaatcgattc tgctatcagt
    12061 caaataatcg atcatattac cagaagtcga aatgtagatt tcacgctgtc ccatatctaa
    12121 tagcaatact gagccatttt gatcatttcc gactgcgtct cttaagtaat tatcagcaaa
    12181 gtctcttggt tcttctgaat tagatgtagt cgttacgatc aagactcgtc ctttgatttt
    12241 ttcattcagc ttttctgcct gttgttccaa agattggatt tgatctgttg tgaataaccc
    12301 agcctcatca tttaccgtag ctgtatctgc atgagtagcc accggccagc caacacttac
    12361 tagcaaaaga aagaataaaa aaaatttttt catcatatca agtaacctcc gatcagaaag
    12421 aggatcgcta gcccagcgaa aatcccaaga gcggtcagtc ccaatttttt ctggctgatc
    12481 ggcaggacac cgctgacctt tcctgtttgt ccattcattg cgtagtagta gactttttta
    12541 cttgagtcat tgcttcgata agtaacaagc cataccggca gtaatacgta attatttact
    12601 tcgtttgtga ttgaagcaga agttcgcaca ccggtcaatg ttgtatatcc atttgcggta
    12661 tcttttaaca gcttctctga atagtctttt aattcagatt gaatctgtga tttgattgcc
    12721 tcatattcga tatctctttt ttctgcttgg aacccggcta aatactggct tttaaaatca
    12781 attgctttat ctaacgggaa aggttgcacg ccttcgacca tcttttgttg cgtatttttt
    12841 gataaggcat tttttactag ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat
    12901 tgcttcgttt ctgtgtattc gatgtcacct atccgccata tgcggataac tgtaccattc
    12961 gcctgcatgg caccatctac ttcagcatct gtagcccaat aagggaaata cacacctgtc
    13021 aacttatcga tttgatcctt attgaaaaaa gctttgggaa caaaccattt tttcttggtc
    13081 caagcgagaa atctgttgat tgcttcttct ttctcgacag caaaaggaag tactttattt
    13141 ggcaagaatt ctccgcttaa acgtcccgat agtacgacgg gattatggca gtagtaacaa
    13201 taagtcgcag cagttgtcgc atcagtcacg atctctgcac cacagttggg acaaagaaaa
    13261 agatctatcg ctgcggtatt atctgcagat tcgtccgctg tatctgaaaa accgcctgcc
    13321 tcgtcaaccg ctttgcgctc ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa
    13381 gtaaaatctt cggtaggtga aggttcttct ttgctttctt cgccagccat acgcgcttct
    13441 ttttgctttt gctcatattg gctgacttct tcttctgtat agatattcag gcagtattcg
    13501 caatgaaatt tttggtcttt gggatcaaaa gtcaacggac cgccgcaatt aggacattta
    13561 tgtgtaagaa catccatcag ctgcacacct ttctaaataa attaattaac ggggattcct
    13621 ttaaacggtt taccacagtt tggacagaat ttagggatgc cgttggaaag atccacgatc
    13681 tcattgcatt cactgcatcg catttgtagc ttaggctgaa cgttttctac aggtttggca
    13741 gctccgcagt tggaacagaa ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa
    13801 gtatccgcat ttccttgtgc agtttgttga ttttgttttt ctgcttgttg tttgatttgt
    13861 tcttggttat tttgagctgc ttgagaaaga taagaaccat ttgcattcat tcccatacct
    13921 acacccataa agccggtcat tgctcctgct tcattttttc cggctgcttc cataccacgt
    13981 gcaatggaac cttgcacgta tccttcacgt acgctaggat cacctagcat cgccccttcg
    14041 ttacgcatat tgatcagttt cacagaatca tcggtgtaag aaatgctggc tacagccaca
    14101 gaaacaatct ccatcccacg taattcacgc caagaatcat ccaatgcagt atccatgtat
    14161 ttgcttaatt ccaaactctt agatggtaca taagaaatcc gttgaccatc tgcagacatc
    14221 tgattgattg ctgattgtag cgctgttagg aattcagcta aatactgctc attgatatca
    14281 ttgatttcca cttgtgtttt gtttttaggg attgcgtttg tataaaataa aatcgggtca
    14341 acgacatgaa tagaataagt gccatgtgcc cgtaagaata attctgcgtt atagaaattg
    14401 tcaaagtagt tcaacggcgc gcttgtaccg aatttgattc cctttatttc ttggagattg
    14461 atataaaaaa cttgttgttt ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt
    14521 tcagcaattg cttctttcag tgttccattg aacatggaag gagctgcgtc atttttaatg
    14581 gtgtaataac cttcctcagc ggtataatcg atgatttttc caccatcgac caaaagcatc
    14641 atcatgtttg gatagacatg gacgactgtt ccgtctgtta aaacatcttc tgttccttta
    14701 cgattggagc cacgtttgtc atctttgcgg actttgacac cttttgtcat gaccgttgta
    14761 tcactcatat tgtctggttc gatgacttct agccattgat ctgccagacc gccgccaacc
    14821 atacttgttg ctgctttgat aagacccata aaaaattcct cctctgattc agctagtgcc
    14881 aaacaattat tgtttattta ttataccata ggaagatgtg aaacttctcg gactagagac
    14941 ggaattgaaa tggaaaattg cgtattgttt aaatacctga aaaagccgag aaggatagta
    15001 gtttttttac ctaatatcac gtataataag agatagtgaa ttttttaaaa aagggggatc
    15061 cttatggcat atcaagcact ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt
    15121 ggacaaaaag ctgtgacaca gacactaaaa aatgctattg tttcccacaa aacctcacat
    15181 gcctacttgt ttacaggacc cagaggaacc ggaaagacga gtgcagcaaa gattttcgca
    15241 aaagcaatca actgtccgaa cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt
    15301 cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac
    15361 ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg
    15421 tacaaagtgt acattattga tgaggtacat atgctttcaa caggagcttt caatgccttg
    15481 ttgaaaacat tggaagagcc aaaagaaagt gtcatattca tcttagcaac gaccgaacct
    15541 cacaaaatac cagctacgat catttcacgg actcagcgat ttgattttaa acggatcaat
    15601 acggcagata ttgtcgaaca tcttgaattt atcttgaaag aatcgactat cagctatgaa
    15661 ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg ggatgcggga tgcattgagt
    15721 attcttgatc aggcaatctc gtttagtgaa ggaacaatta ctttagatga tgccatgcaa
    15781 gtgacaggta gcttaactga cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa
    15841 gaagtaccag aagcattgga aacattgaac caaattctcg cagccggcaa agaatcacgt
    15901 cgctttctag aggatatgtt acaatactgc cgtgacttgc tgatgtatca gcaagcacct
    15961 aacctagttg aggaacaatc gagtacgatc agaagtacat ttacagaatt ggcacaaact
    16021 gtttcagcgg atcagctgtt ttcaatgata aagattttga gcgatactca aaatgaaatt
    16081 cgttttacca atagtgccgc tatttatttg gaagtcgcta cagtcaaact tgcacagcta
    16141 ccgtctgctt ctgttccttc agcagatgta gcatttgcag atgatcagat agtaaaacag
    16201 ctacaacaag agattaagga actaagaaac gaaatcaagg aactacgcag taatacagga
    16261 attgagaata caaaagtaca gccggtacag caaacaaatc ataaaaaaca gacaagtacc
    16321 tatcgtgtac cgacggaacg agtatataaa gttctgcggg aagcgacaag acagcatctg
    16381 aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc tatctgtcac tcagcgagca
    16441 atgcttaaag caagcgaacc agttgcagct ggacctacag gactagtgat tgcgtttgat
    16501 tatgagattg tttgtcaaag agcagcaaat gacgaagaat tgcagctttc ggtacacaac
    16561 aacttaagcc gaatgatcaa agattatgcg cctgatcctg tttatattac aagagaaagc
    16621 tggccagaac ttcgaagaaa ttatttgacg caggcacaag aagagggaac agaaaactct
    16681 tttgcttctg aagactcaga aggagaaatc gagttgcttc ctcaagaaca agaagtacca
    16741 gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct ccgaagctgt tacagtggta
    16801 gatgattaat aaaaaggagt agataattat gatgcgtgga atgggcaata tgcaaggcat
    16861 gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa
    16921 aacagagttt acaggtaaag ccaccaatga attggtcaaa gcggttttta caggcgatcg
    16981 tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat cctgatgata tcgagatgct
    17041 tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca caaattgatg tggaaactga
    17101 gaaaaaatta gggaaatacg caaaaggttt gccaggattt tagttttaaa ctgaaggagt
    17161 aatgaatatg caatatccag aaccaattgc gaaactgatc gacagctaca tgaaacttcc
    17221 aggaatcggt cagaaaacag cgacaagact cgccttttat acgatcgata tgaaagatga
    17281 agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag
    17341 tatctgcgga aacatcacag aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga
    17401 tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg
    17461 agaatatcac ggactttatc atgttctcca tggtgtgtta tcaccgatgg aagggacagg
    17521 tccagaagac atcaatattt cttctctgtt gcagcggctg catgatgcag atgtaaaaga
    17581 agtgattatc gcaacaaatg caacaacaga gggagaagca acagctatgt atctctcacg
    17641 tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca catggtctgt ctgtaggctc
    17701 tgatatcgag tatgcagatg aagttacttt attgaaagct gtcgaaggaa gacgtgagtt
    17761 ataaatttct aattaagtgt tttttcagat acaatataaa agcagcacaa gagatggggg
    17821 caacaatgtg aacggactat ttattacaat cgaggggcct gatggtgcag gaaaaaccag
    17881 catcttgaat gaactttacc ctcttttgaa agaagtagca aaaacagaaa tcattcaaac
    17941 aagagaacca ggaggcattc cgattgctga agagatccga gcagtgatcc ttgatccaaa
    18001 aaatgatcgg atggatgaac gtacggaagc actgctttat gcagcagcaa gaagacaaca
    18061 tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa atcgttttat gcgatagatt
    18121 tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc atcggcgtta ccgagattgc
    18181 tcggttgaat gagtttgcta cagaaggtac tacgccggat tttacccttt atcttgacgt
    18241 agattcagac accggattaa gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt
    18301 agattcggaa ggtttggaat tccaccagcg tgtacgtcat gcttacttaa agctggcaga
    18361 agagaatccg gaaagaatcc acaaagttga tgcaagaaaa agctttgaag aagtgctgca
    18421 aacaagttat catacaatta ttgaacagta tccgcaattt tttgaaaact aggaaggtga
    18481 acgtttatga aaattatttt agcgattatc caagacaaag acagcaaccg tttatcaaat
    18541 gaattgattg atgcaaatat ccgtgccaca aaactatcat cgacaggcgg cttccttaag
    18601 gctggaaaca gtacgtttat cgtgggtatt gaagatgacc gtgtagaaga agcattggaa
    18661 atcatcaaaa aaacgtgtga atcaagaaag caatttgtat ctacacctgt tacactggat
    18721 atttcgatgg atgggggcgt cccttatcca gtagaagtag aagtaggcgg tgccacagtc
    18781 ttcgtcttac ctgttgaagg attccatcaa ttttaggagg agctgagcta tgaatcaaga
    18841 aataaacttg gctcaaatgc aaccgatcgt gtatcagcag cttcaacgga gttttgagca
    18901 tgggcgtatc gctcatgctt atctttttga aggggagaaa ggaacaggaa aacatgaagt
    18961 aggtatatgg cttgcacagc acctgttctg tacccaaatg aaagacaatc tgccttgtgg
    19021 aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat ccagatgttt tggttattga
    19081 accggaagga caaaccatca aagtcgatca gatcagacgt ttgcagacgg aattcagtcg
    19141 cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag
    19201 cagtgcggca aacagtttgt tgaaattctt ggaagagccc cctggagatt ttttagcggt
    19261 gttagaaaca gatgcagtag gaagaattct ccctactatt caatctcgtt gtcaagttct
    19321 gcattttcaa gaactttcga aagaagcact gatccatcga ttgcaagaag agcagattcc
    19381 attggaaaaa gcaaaacttt tggctttctt aacaaacagt ttaggaaaag cagttgaaat
    19441 ctctcaagat gaatggttta atgatgctaa ggacttgatt tatcaatggt tcgtttactt
    19501 gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa aaattggtaa aatcatttaa
    19561 agaaaaaaca caacagttta caggattatc tgtcttgatg ttttattatc aggaagcaca
    19621 gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa cagataaacc agataattga
    19681 acggatattg atcgcagagc agaaactacg cagcaatgtc agttttcagg ctgttgcgga
    19741 acagttcgtt ttgcatacga ttttttagct tgccgcaaag gatgatgatc aaggaggatg
    19801 gaaatggtag aagtagtagg tgtccgatat agagatgcag gtcatatcta ttattttgca
    19861 ccaggagact ctgaatatgt ttatagtgac aaggtattag tcgaatctca gcaatcctta
    19921 catattgcta ctgttgtcct tcctaaaaaa gagattgatc ccaatgacct tcctgaagct
    19981 atccgtccga tcttgcataa agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa
    20041 aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa
    20101 atgaaaatgg tcaaagccga atacacattt gatcgaaata aattgatttt ttctttcaca
    20161 gcggacgggc gaattgactt tagagaattg gtaaaagatc tggccgctgt atttcgtact
    20221 cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa agataatagg cggaattggt
    20281 ccatgtgggc gtccattatg ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc
    20341 aaaatggcaa aagatcaaag tttatctttg aatccaacaa aaatttccgg tttatgcgga
    20401 cgcttgatgt gctgtctgca atatgagagc gaagaatacg agacagcgaa aaaagaactc
    20461 cctgactttg gaaaagtaat tgctacacct gatggtaaag ggaaagtcgt agggttgaat
    20521 ctgcttagcc gtatcgttag tattcggctg actggacggg aaaatcctgt cgaatatgaa
    20581 tgggatgaat tgaaagaatt tatccaagta ggtgagatga atggataaga gatcattata
    20641 cgacggattg aatcaattag aatcagagtt gagaaataca ttaacagaac ttgttgaaat
    20701 gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg
    20761 tctgcgggaa cacctgcggg aattgaacca agttgcacaa gctccagctg aaaatgtgaa
    20821 gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt tatgaagaag gattccatgt
    20881 ctgctacgat ttatatggat caagaaggga caatgacgag ccttgtgcgt tttgtctaga
    20941 agtgatctat cgcgaaagcg gtcgatgacc aataacaaga ggagactgga gcggagaaat
    21001 actgctccag tctttttatt aaggagattg tgacagatgt ataatcaaaa aagtttcaag
    21061 ggaaatccgg tatatgggga tttatatctt gtaccgacgc caattggaaa tttagaagat
    21121 atgagctaca gaagtgttcg tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg
    21181 cgtaatacac aaaagttatt gaatcatttt gaaatccata caccccaaaa aagtttacat
    21241 gaacataatt ataaagaacg tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg
    21301 attgctcaag tcagtgacgc aggaatgcct tccatcagcg atcctggaca tgagctagtt
    21361 ttagcctgca taaaagaagg gatttctgtg atctctatcc caggacctac agctgggatg
    21421 acggctttga ttgcttcagg gctagtgcct caaccatttt tattttatgg cttccttcca
    21481 cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa aagaacagac agttacgctg
    21541 attttttatg agtcacctta tcggatcact gctacattga cgaatttctt agaaattttt
    21601 ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga
    21661 ggaagtacgg aagagctact cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt
    21721 ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg aagttgatga agagttggga
    21781 acgttaaagg aacaagtaga agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc
    21841 aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa
    21901 ttagactgag atagtataag ttgtctataa tgaaagacta atgaggaaat cagctactaa
    21961 cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata atgaataaga ggtgaggatg
    22021 atggagctgc aatttccttt acataatgat acatcaagaa aaatcattca tattgatatg
    22081 gatgcttttt tcgcttcggt tgaagaacga gatcatcctg agtttcgcgg acatccatta
    22141 gtaattgcca gacaccccag tgacactggt gggcgtggag tagtgactac agcaaattat
    22201 gaagcaagaa aattcggaat tcattcagca atgagtgcac aaaaagcgta tgagctttgt
    22261 ccgcaagcca tctttaagcc tggcgaccat cataaataca gcgatatttc caaacaagta
    22321 cgggaagtat tctatcgtta tacagatatt gtggaaccag ttagtatcga tgaagcgtat
    22381 cttgatgtga ccgaaaataa aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc
    22441 caagcagata tttgggagag tgtccagcta acatgttctg cgggagtaag ttacaataag
    22501 tttttggcca aattagcatc agactatcaa aaacctagag gaatcacggt agttgcccct
    22561 caagacgctg tgccattttt aaaagctttg ccaattgaaa aatttcatgg ggtagggaag
    22621 aaaactgtac ctagaatgca tgagctaggg atttatacag gaaaagacct atatgaatgc
    22681 acagaaatga tgttgattcg aaattttgga aaaatgggct attctcttta tcgtaaagtc
    22741 agaggaatcc atgattctcc agtaaacgtt acaagagagc gaaaatctgt gggaaaagag
    22801 catacttatg ggcagccgtt gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta
    22861 gctgaaaaag tagaagaagc actgagaaga gtccaaaaac atgggaaaac agtagttttg
    22921 aaggtcaggt acacggacta ttctactgtg accaaaagag tgacattgcc agaatatatc
    22981 tataaaaaag aagcactctt ttatcaagca agccttatat gggaggaaat cttgggagta
    23041 gaaaaaggaa tccgattgtt agggattact ttgacgaatc tggacccgat gacttatgaa
    23101 aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa aacagctttg agagttgaaa
    23161 ccggaaatcc gttttctctc ccaaagctgt ttttatatca agtcttgctt tttgatttgt
    23221 tcataggttt ctgttgcatc cgtacatttt tcccaaatca caacttgatt ggattctaaa
    23281 gatttttgga cgtcaatgat ccgctggttg ctacttccgc gaaattgtag ttttaggtca
    23341 cgtttgctta attcaaagcg tccgtcaaca aggatatcga tttgcgaaag gagttctaat
    23401 ttatcttctg aatcttgtag caattcatca aaggtatagc ctgtccatga ccagatatct
    23461 tttgtcgtac cgaactcttt tcggatacgt cgtacgactt gcagacaaac atctgtattc
    23521 aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt aatcgttgcg caagtcagcg
    23581 atgatttgtt cttctaattc aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct
    23641 ttgttgaagc agccttcgca ggcaaataag cagccactaa cgtaaagact gttgcggaca
    23701 ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa tatagttttg gctgtagtct
    23761 tttgctaacc attcttttgg ttttggattt ctcataaaaa aacttctttc atttaaggtt
    23821 caaagatata gctattgaag ataaaatcta ctcgaccaca taaataagaa caaactgacc
    23881 taaatccatt gttcaagaaa gcttgttctt attttgatag agggcgttca aagatacaaa
    23941 gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc tcttattctg aataaacggt
    24001 gttcaaaagc ttgcatctgc agtaataagc ccaactaaac gaaaaatatg aggaactatt
    24061 tttgtttagc tattcttatt acttgatgca ggacagcttt ttcacaacct catttcatgt
    24121 gtttcacacg tgaggaaatt tctttatggc ggccatgtac catcggacga gcttgcggat
    24181 ttcctaaata gccacaagta cgtttgacta catcacatgt ttttggatca tgatttccac
    24241 attctggaca ttcgaaccct cgttctgtcg gtttgaaatc accttcgaaa ccacattgat
    24301 agcaatgatc gattggcgta ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc
    24361 atacagcttc taaagctttc ggattttgtt ggagcatagg gtattcacag tagtggatga
    24421 atccacctga acaatatttt ggataatcct tctcaaattc caatttttca aatggcgttg
    24481 ggtttttacg cacatcatag tggaaactgt ttgtataata ttctttatct gtgatatttt
    24541 cgatgatgcc gaatttttct gtatccaaac ggcagaaacg gtcagttaga ctttcacttg
    24601 gagttgaata gacactgaaa tgataaccat attcatcccc ccatgcatct gcattagctt
    24661 ttaattcttt gacgatatca agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc
    24721 caccgaagaa agctgaagca acttcataca atccaatgta gcctaacgat acagtagctc
    24781 gacggttttt gaataattca ttgacagaat cggtacgtga aagacgtttc ccgaaagcac
    24841 catacatata taaaattggc gcatttgctg gaatggcttc tttacaacgt tctacacgat
    24901 agagaagcgc atctttcatg atttctaagc ggtcagacag taactgccag aatttattct
    24961 gatctccttt tgattcgatc gcgattcttg gcaaattcaa tgtaacaaca cctaaattca
    25021 ttcgtccgac atttacttct tgaccatttt cgtctttcca cccttgcaag aatgaacgac
    25081 agcccatagg tactttgaaa cttccagtta attcaacgat tttatcgtaa ttcagaatat
    25141 caggatacat tcttttcgtt gcacattcca atgctagttg tttgatgtcg taattaggat
    25201 cttcagggtt taagttcaca ccttttttca aagtgaagat cagtttaggg aagatagctg
    25261 tccgcttttc actccctaat ccattgattc gaatctgtaa aattgctttt tgaatttctc
    25321 tttcaaacca gttttctcct agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg
    25381 aagtaaataa ggtattgatt tcatattcca aactttgcat agcatcgaag atatcttttt
    25441 tggtttttgc ttttgcatat tctttttggc gttctggcga atcgatccat tcttccgcgt
    25501 cttttagatg ttttttatag ttcaattctg caaaaggagc aagcaattca tctgttcggt
    25561 cggcagaaca gccaccatat tgacttgacg ctacattggc aataatctgt gagatttgtg
    25621 ctgtggcggt ctggatagat tttggcgatt cgacttcggc attgccgatt ttgaacccat
    25681 tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt catcggtgta taaggatgat
    25741 aatccaaatc atggtaatgg atatcgcctt tttgatgcgc atttgctata tgtggtggca
    25801 acattttcag tccgattgat tttccaacga tcccggctgt cagatctcgc tgtgtattga
    25861 aaacattact gtctttattt gcgttttcgt tcacgacagt ttggtccttg ttgattagct
    25921 tttcaatcga aaagttgata tctgttgctt tactgcgagc aaaatctcgt tttgtacgat
    25981 aattgatgta ctcttctgct aattcatatt cgtttttttc tagtaatata tgttccacga
    26041 tgttttgaat ctcatagatt ttgacatttt cagcaaaccg gcttttgatt tcttggacaa
    26101 ccaagtctgc aatcatttga attttttcat aagtaagtgg tgataatgag ccgtgaattt
    26161 tcttttctgc tttgatcagt gcttcattga tcttttgttc atcgaaatca acatggcggc
    26221 catcgcgttt gatgactttc atctgggatg tctctggctg ctgagtaacg acagtttttt
    26281 cttcataatt tactttcatt atcttcaact cctgctaaat tgttcttgtc tatcataaaa
    26341 caaaatcggg gaaggagcaa ctaaatatcg gctatatata gtattcaatg tgctgtataa
    26401 ttggatttct atactatatg tagtgtatat tttattgtga aaaaaggtga aaacttgtag
    26461 ctggtggatt tgttttttgc atatttaatt ttgctgaata aaataattga ataaaatttt
    26521 ttattcagtc caagtaaaag tgacaaaaac ataagagcaa attattcgta gatatgctac
    26581 tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga agaattggaa aagatgatcg
    26641 aagtgaaaca tctaagtaaa agctacggca gcaaactttc tcccgtcaaa gccttagata
    26701 atatctcatt cacagtagaa gaaggcgagt ttgttgggat tatgggccca agtggtgcag
    26761 gaaagacaac gctgatgaat atcttgtcca cgatcaattt gcctacgatg ggcagtgtct
    26821 cgatccaagg aaaagaaatc acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa
    26881 aaaaactagg attcattttt caagagttca atttgatcga taccctaaat gcaaaagaca
    26941 atatcctatt gcccctagca gttgaacgga tgactaaaga ggaaatggaa aagagagtaa
    27001 gacatgtagc tcaattgttg aatatagaag aactgctcaa acggtatccc gatgaacttt
    27061 ctgtcggaca gagacaacga attgcagctg ctagagcact tgttgttcag ccacaagtga
    27121 tttttgcaga tgaaccaact ggctctttgg actcgaagtc ggctacagaa ttgttgaact
    27181 acttgaagac catgaatcaa aaagaaaaag caacgatact gttagtcacc catgatccat
    27241 atacagcaag ctactgcgat agaattttat ttattaaaga tggtgtgatt ttttctgaag
    27301 ttgtcagaag agggacaaga agagagttct ttgagaaagt aatagacatg caggcgacga
    27361 ttggcggagg aggaaaaatg aatgctgtat aaactagccg ttaaaagttt ttttcggcag
    27421 tcacgaggct atcttgttta ttttttcagt ttgactttat ccgtaatgat ctattattcc
    27481 ttcagcgcaa tgacctacga tcaatcttta gtcagaagag caagtcaaga tacttcgata
    27541 gatggtggat tgagttttgg tggatttatc attactgttg ttttgctttt tttcgtattc
    27601 agtgccaacc gatttttctt gaatagaagg caaaaggaaa taggcatcta tcagcttttt
    27661 ggtatgaata aactgcagat atcaggtata tatgtgttag aaatcatgat aattggattg
    27721 ttcgcctgta tttctgggat attactcggt attatttttt ccaaactctt ttcaatgatt
    27781 ttggttcgaa tgatggatat ggatcttact agtccatttt ttatctccat tccatcagta
    27841 gtagatactt tatttgcttt tttcatcatc cttttagcag tatctgtata ttcaatatgg
    27901 aaaatatggc gctatccaat gattcgaagt tttggagaaa aagaacaagc agatagttca
    27961 acaatgcgct tcaggacaag gcaccgatta ctcgcagtca tcgggttact acttttaacg
    28021 agcggatatt ttggagctag tcattttagg gaaatcactt catggatgat cagttatcat
    28081 tatcttgata cattagtttt cttttttcct tttttaattt ttttcgtttg tgtggtcggt
    28141 acttatttgt ttttctgtta tacatttcgg ttatttttga tcatattgag taaatggaag
    28201 atttattact atggtatcaa ttttttgata acgggtaaca cgcaagttca tttgctcaaa
    28261 agttggcgta caaattctct gattacagtc attctcggaa tttctttaat gacaattggc
    28321 ggcacggcta gtatctctac tattttatcc taccaggaga aagtttcctc cccgatggac
    28381 tatcagtttg atgttgagac agagaaaaaa atcaagccga ttctagcaga ggaagggcag
    28441 aagataacta aagaaatcac cttgcattat aaagtcattg ggagtatcta tagtcaaaat
    28501 ctcgaagaac ctatgtttca aactgctaat ctgatcacgg aaacacaata tcaagccttc
    28561 agaaaagtta atccagattt gccgaacatt tctttgaaat cacctggtca tactgttttg
    28621 cttgatcaaa tgcaaactct ttttcggaat atttcattat atggatctag tattggactg
    28681 ccagataatc aagaattgaa gattcaacaa atgcttccaa gtgtattagg tgacgaaaca
    28741 atggtatatc taagtcctgt actgattgta gatgataccg tttttgaaaa agcaaaaggt
    28801 gtggattatc aagtagtcaa tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa
    28861 cgactgagca atgacctgtc gataaagtgg ctcaatgctg tttattattc ttatgatatc
    28921 gtaaatggaa gtctacaagg agaaattagc acaaagaaac ttccggatga ccaaatgaag
    28981 gaagcacaat ttagccaaga aagctctcgt ttgaatttct cttccagcta ttcgaaattg
    29041 cgtgcagtta atcggcgaat cggtatcaat atttttgtca cactatttgt cgggatgatc
    29101 gtgattattg ctactggtag tattttgaca gtccggcaat tagcagaagc agaggaagaa
    29161 aaagaaaatt atcacctatt gactaagctg gggattccac aaagaaggat cagacggatg
    29221 atcttcgagc aaaacgcact taccttcttt ccacccatga tattagggat cactcacgca
    29281 gtatttgcaa tcaatgtctt cactcagtat ataaaaactg ccgattactg gttggcttat
    29341 cttgtgagtg gcttattgat ccttatctat cttttgcttt atgtcattac gagtcagtta
    29401 tattgtcgga ttatcgagga atgatatacg gaataagaca agataataaa tagaactaaa
    29461 ttttgaatac catttatttg gaataagagg ttgtgacacg atttttgtca caacctcttt
    29521 attttttata ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg
    29581 ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc tgtaagctag
    29641 acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat gaggagggaa
    29701 tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat tcatcattcg
    29761 aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact ataaaaatat
    29821 tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa atcaaaacga
    29881 ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct tatcaggcgg
    29941 atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct tattatttag
    30001 aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag tttgaaatga
    30061 ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt gaagagcata
    30121 cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg gtcatcagta
    30181 tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag
    30241 aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt acagaaaatt
    30301 tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa atcagtcatg
    30361 ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag cctggtatgc
    30421 atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt tatcctgcat
    30481 tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac tttcgtcaat
    30541 tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca tcatttatgg
    30601 atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg gacatgtttt
    30661 tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa
    30721 atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt tcaagtattc
    30781 agattgacca gcatgtacta gatagttatg accgactacc gattggctac gtaaaaaaag
    30841 acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa acgggtgaaa
    30901 tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa aagacagctg
    30961 ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca ggtagactag
    31021 atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa ttacatggat
    31081 accggattga actcggggat atcgaacatt atctgctcca agacaaccga atcaaacaag
    31141 ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct tttgtcgtat
    31201 tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa gaacaattac
    31261 tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat ctgctgcctc
    31321 agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat
    31381 gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag ccattgcgct
    31441 tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt atcaatcagc
    31501 agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac agggagcggc
    31561 gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg tttatcggca
    31621 aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa ttccactgtt
    31681 tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg gttttttagg
    31741 aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag atggcatgat
    31801 caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa caatctcttc
    31861 tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc ctgttcgtga
    31921 taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa
    31981 attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac gcattgcctt
    32041 acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca gtatgtattt
    32101 gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca tgatgggata
    32161 tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag aattctggaa
    32221 tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc gattgatgtt
    32281 cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata tcggttattt
    32341 tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca tcgtatatgg
    32401 actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta agaagaagca
    32461 taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt tgacatttca
    32521 agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt tttttagcta
    32581 acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga agaacaaatt
    32641 ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat
    32701 ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt cgagctagac
    32761 ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg gagcacaccg
    32821 aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag aaactttttg
    32881 ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt acctcctcct
    32941 atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga
    33001 acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa tatattcctt
    33061 tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt ctggcagaaa
    33121 aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa tcgttaagcc
    33181 aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc gtctttatta
    33241 tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat acgtattatt
    33301 ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct gctgatcgct
    33361 atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg ttgacggcga
    33421 ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat cagctgcgca
    33481 gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg ttgaaagatc
    33541 gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa gaaacagaat
    33601 tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca ttcgggttga
    33661 aaaacgattt ttatacgcat cgtatccgag cccacgagcc agagctcaaa cagtcacaaa
    33721 aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc ttggatcaat
    33781 tagcaaaaaa ccataacgaa gtattgttca tcatcccacc ggttaacgag aaatggagcg
    33841 attataccgg actatctcaa gaaatgttac aaggatttgc caaaaagatc aaatttcaat
    33901 taaatagcca aggattcaat cgaatagctg attttgtcaa tcaagcgggt accaactatt
    33961 ttatggaaga cacgatccat ctaggctgga aaggctggtt ggctgcagat caacagatca
    34021 gaccgttttt agaagaaaat catattactg ctagtaagta tcatcttgac gatgcttttt
    34081 ttagcaaatc ttggcaacat caaataccag ataaactaca attaaaataa agaaaacagg
    34141 cgagtgttgc tcgcttgttt tctttatttt tgaatattct gaataaaaga taaaaatacc
    34201 aagtttgcct gaacaaaaat cataagcaat ggtataatca caatatgaat atagaaatgg
    34261 gggaaaacat ttggatacaa aagaagaaca gctacttatt gatttaactt ctgcaaaagg
    34321 agtacctgga aatgaagaag aggtaagaga agtattcaga gaatatgcca aaccattcgc
    34381 tgatgatatt ttttatgacg gtttaggcag tgtgattgcc aaacatggtg ctggcggagg
    34441 ccccaaggtc tttatttctg gacatatgga cgaagttggt tttatggtaa caaaaataac
    34501 cgaaaaagga ttcttggaat tccaaactct tggaggttgg tgggggcaag tcatgcttgc
    34561 tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa
    34621 accaccacat gttttaacac ctcaagtaag aaataaacct tatgaaataa aggatatgtt
    34681 cattgatatt ggcgcatcaa gccaagaaga agccaaagag tggggcatac gtcctggaga
    34741 tatggtcact ccatatattg aatacaaacg aatgaatggc agtaaatatt tactggcaaa
    34801 agcatgggat aatcgtatcg ggacagctgt ttctctgcga gtattagaga atctatctaa
    34861 agaagcacac ccaaatgttt tattcgcagg aagcgatgtg caagaagaag taggcttgcg
    34921 aggtgctcga acaagtacac accttgtaaa tcccgatatt gcttttgctt tagatactgg
    34981 tacagcagga gacactccag gaatgacacc aaaagaagca gattcgattt taggaaaagg
    35041 cccacagatt ttgatttttg atgcatctat gatcccgcat aaaaaattat tgaattttgt
    35101 tattgatgta gcagaggaaa tggatattcc attccaatat acagtgattt ctggcggagg
    35161 aactgatgca ggacaaatgc atttgacaag agatggtgtt ccgtctgtag ccattaccgt
    35221 tcctgttcgt tatctgcatt cccatacatc agtgattcat gaagatgatt atttgaatac
    35281 agtgaaacta gtcacagaat tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg
    35341 gagttatgaa taatgaataa tagaatacct atttctgaat atcggaaaaa agcacgagtt
    35401 tctttggaaa atcaatgggg aatcaatact tggcttgttt tcttatctat aatatttacg
    35461 agcgtcatcc agaacttttt tgacggtttc agtccttttc cggaagaaag catgcagcag
    35521 cacttattga gttttctagt cgataattta cttttattcg cattcaacta tgcattgttt
    35581 tacattgcgc tagtagttgt acggggtggc agaccaaaaa gtaacctttt attttcagtt
    35641 ttccaaagaa agtattttgg ttcgatactt ggcatcaatt tgatcaattc agtcatcaac
    35701 tggctactca acagtttaat ctttctgccc atgtttttaa tcagcggagt gagtacttat
    35761 atgcagctgc ttttcagttc aaatgcggta cctacgggtg tcatgccaga agattttctt
    35821 gatatcagct ttgttttatc gatgataggg atgatttttg tttcagcact catcatgagt
    35881 attgttggcg gtttattcca atttgctgca tggacaaagt tcgactatcc tgatttgtcg
    35941 gttattcaat gtttgaaata tggctggttt ttattgaaag atagaattgg tacgtatatt
    36001 ttgttgcagc tgtcttttat cggctggtat ttgctaggag caatcgcttt gttcatcggt
    36061 cttttgtggg tcattgttta tgtgaatgtg tcagttgcag aattttatga acaagcacgg
    36121 attgaaaaag gaggtccaga cgcctatttt acttagcatg taagcagttt attcatcaaa
    36181 aaaataactt ttgtggtagg ataaatcttt gagggggaat gtacgatgaa attaactgtt
    36241 ttaggatgct taggagcgta tccttataaa aatcaaggga caacaggtta tttgcttcaa
    36301 tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa attacaagaa
    36361 caccttgatc cacttgatct agatgctgtg atccttagcc attatcatca tgatcatatt
    36421 gctgaccttg gtgtattaca gtattactgg caacttcatc ctaagagaaa tccggagaca
    36481 ttgctttcaa tttatggaca cacaaaagat tccattcact ttgaagaatt gacgttggaa
    36541 aatgtctcaa aaggacatcc ttattttgaa gctgaagaat taaaaattgg tccttttctg
    36601 attactttta tggaaacaat ccatccagta gtatgttacg ccatgcgtat cgtagaagaa
    36661 acaacgggga aagtattcgt atttacaggt gattcaggtt atttggaaag tttcgctgat
    36721 tttgcgaaag atgctgattt gtttctagca gatacctatc tttttgaagg aaatgaacat
    36781 catcatgccc atttcacttc aaaagaagct ggagaacttg cgaaatcagc taacgtaaag
    36841 aaacttgtac ttacacacct acctcagttt ggtgatttag aacaattgaa aaatgaagcg
    36901 caaaaggcag caggcaaagg gattgatata gaacttgctg cagttgacaa agtgtttaac
    36961 atttaggagg aagaacatga tttttgtacc aaatgaaaac cacgacccaa gaatcaattt
    37021 agcaattgaa caatatttgc tgcaagaaat gcctttagat gaacctattt tgctctttta
    37081 tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg
    37141 agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt
    37201 ctaccatgat tttggaaacc tgaactttag ttttatcatg cctgatgatg gggattcatt
    37261 ccgtgacttt gcgaaagtaa cacagccaat catccaagca cttcatgaat tgggtgttgc
    37321 tggggcggaa ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat tttctggcaa
    37381 cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga acaattatgt tcgactctga
    37441 tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat
    37501 caaatcgatc cgttctcgtg taacgaatat caaaccattt cttccagaag aaaagcaaaa
    37561 aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa attttcggcg tagagtcagt
    37621 agaagaagta aaaacgtacg aattgactga tgaagattgg aaaaaaatca atcgaatctc
    37681 tgaggaatat tatcgaaatt gggattggaa ttacgggaag tctccagatt tcaatttctc
    37741 tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat ttgaatgtct cagaaggaat
    37801 gatcaaagaa gcaaaaatct ttggcgattt cttcggacta ggagatatct ctgacgtgga
    37861 aaaaaaattg gttggacaga aatatgataa agcatctcta tcaaaaatag tcagccaaat
    37921 tgatgtgaag aaatattttg gtgcgataga accagaagaa ttgcttacct tactttattg
    37981 aaaataaaaa a
//
LOCUS       DAZUMO010000024        35881 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000024 DAZUMO010000000
VERSION     DAZUMO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.024"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8ER_001208"
     CDS             <1..1152
                     /locus_tag="ACG8ER_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFS5049804.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8ER_001209"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8ER_001209"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS5049805.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEITILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8ER_001210"
     CDS             2728..3003
                     /locus_tag="ACG8ER_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS5049806.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8ER_001211"
     CDS             3009..3776
                     /locus_tag="ACG8ER_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS5049807.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8ER_001212"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8ER_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS5049808.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8ER_001213"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8ER_001213"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS5049809.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8ER_001214"
     CDS             7920..8222
                     /locus_tag="ACG8ER_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS5049810.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8ER_001215"
     CDS             8225..9388
                     /locus_tag="ACG8ER_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5049811.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8ER_001216"
     CDS             9414..12842
                     /locus_tag="ACG8ER_001216"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS5049812.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISPGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8ER_001217"
     CDS             12863..13987
                     /locus_tag="ACG8ER_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS5049813.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8ER_001218"
     CDS             13968..14414
                     /locus_tag="ACG8ER_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5049814.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8ER_001219"
     CDS             14398..14736
                     /locus_tag="ACG8ER_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS5049815.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8ER_001220"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8ER_001220"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS5049816.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8ER_001221"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8ER_001221"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS5049817.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8ER_001222"
     CDS             15893..16936
                     /locus_tag="ACG8ER_001222"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS5049818.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG8ER_001223"
     CDS             17095..17823
                     /locus_tag="ACG8ER_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS5049819.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG8ER_001224"
     CDS             17939..18184
                     /locus_tag="ACG8ER_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5049820.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG8ER_001225"
     CDS             18181..18399
                     /locus_tag="ACG8ER_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS5049821.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG8ER_001226"
     CDS             18535..19074
                     /locus_tag="ACG8ER_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS5049822.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG8ER_001227"
     CDS             19067..21340
                     /locus_tag="ACG8ER_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS5049823.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG8ER_001228"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG8ER_001228"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS5049824.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8ER_001229"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8ER_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS5049825.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23157..23435)
                     /locus_tag="ACG8ER_001230"
     CDS             complement(23157..23435)
                     /locus_tag="ACG8ER_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049826.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPECLY"
     gene            complement(23697..23825)
                     /locus_tag="ACG8ER_001231"
     CDS             complement(23697..23825)
                     /locus_tag="ACG8ER_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049827.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23905..24360)
                     /locus_tag="ACG8ER_001232"
     CDS             complement(23905..24360)
                     /locus_tag="ACG8ER_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS5049828.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24498..26426)
                     /locus_tag="ACG8ER_001233"
     CDS             complement(24498..26426)
                     /locus_tag="ACG8ER_001233"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5049829.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26623..27456)
                     /locus_tag="ACG8ER_001234"
     CDS             complement(26623..27456)
                     /locus_tag="ACG8ER_001234"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS5049830.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27648..28352)
                     /locus_tag="ACG8ER_001235"
     CDS             complement(27648..28352)
                     /locus_tag="ACG8ER_001235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5049831.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28375..29001)
                     /locus_tag="ACG8ER_001236"
     CDS             complement(28375..29001)
                     /locus_tag="ACG8ER_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5049832.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29327..31702
                     /locus_tag="ACG8ER_001237"
     CDS             29327..31702
                     /locus_tag="ACG8ER_001237"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS5049833.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31726..32217)
                     /locus_tag="ACG8ER_001238"
     CDS             complement(31726..32217)
                     /locus_tag="ACG8ER_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS5049834.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32404..33324
                     /locus_tag="ACG8ER_001239"
     CDS             32404..33324
                     /locus_tag="ACG8ER_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5049835.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33478..33822
                     /locus_tag="ACG8ER_001240"
     CDS             33478..33822
                     /locus_tag="ACG8ER_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS5049836.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34012..34512)
                     /locus_tag="ACG8ER_001241"
     CDS             complement(34012..34512)
                     /locus_tag="ACG8ER_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5049837.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35040..35123
                     /locus_tag="ACG8ER_001242"
     tRNA            35040..35123
                     /locus_tag="ACG8ER_001242"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35074..35076,aa:Leu,seq:cag)
     gene            complement(35447..>35881)
                     /locus_tag="ACG8ER_001243"
     CDS             complement(35447..>35881)
                     /locus_tag="ACG8ER_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5049838.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11869 a         6263 c         7404 g        10345 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaaataac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggagggatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgcctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggcatt ctggtgtacc gattatttta atgtattcat cgatattttt tatcaacatt
    23221 ttttttatga ccaactggtt ccgacggttc catttgccac ttacttccag cacatattta
    23281 tttaggggca aacgtaagat atattttgcc aaatagcgat ctttaagcag gccattgatc
    23341 acacggtcct caagctgcaa gttgatccca gtcagcatca ttggctgttc ttgtaaaacg
    23401 tacgtatcaa ttaaaagacc ttcaaaagtt tccacaagcg tcttcctttc ttttcaaagt
    23461 aacctgaaaa caggcactaa ttcttccatt ttgatattcg ttgaagtgga tgcgatcatt
    23521 ctttcttttg tccactgtaa tctagtataa caaaaaagag cagaagagcc aaaaagaaaa
    23581 aagagcatcg aattctatag gaagtctcat tggcacggcc taaattatta caaaacattg
    23641 attaaaaaaa gcccctgcat cgcaggagca tcccctatat cgttttaata ggtcaatcaa
    23701 aaagcagtat caaacacatt ttgtatcaaa tcaggaaata atagataagc taagcccaga
    23761 atcaaaagta ccgctagaag aaaccagccg attgttccta tgaaatactt taatttttcg
    23821 aacattgtat cacctcgttg tcagtatatc acggatcagt aaattggggg tgttaagaat
    23881 ctcctaaaat agacaaaatt tgttctattt tcctaaagca aatacttgta agatacagta
    23941 agtgatacca gaagcaatga ccggtccggc ggcgattcct cttaagaaga caacacctaa
    24001 aatcgtgccg aaaacaagtg caacagtgat ttgtggatca gaagcaatca atcctacacc
    24061 cttggaagat aagacagcca ctagcccacc gcaaaaaaca gctacccagc caatagggga
    24121 tttaaaggct tctaatagat cacgtagacc gatttctcct gttgcgatag ggattagaat
    24181 agccgctgtg atgatcgtca ccccaatatt gattcctttg ccttgaagcc aagggaaaaa
    24241 ggaacgggag gctggtatga gcttcataat caaaacaaaa gctgccgcta tcatcagact
    24301 ttgattttta gctaaaattg ctacaagtaa aataattcct aaaaaaagcc aactttccat
    24361 gaaatatatt cctcactttc ttcgaaacaa tcataacatg tttttagaaa gttacaaaag
    24421 taggaattca tccttcaaga cctattcata agctgttttt ttcataaaat aaacaaaatc
    24481 ataaaaaaac actttgctta tttgacagaa gacagccgcg ctttgtatag tggacaagac
    24541 ttacattttt ttgaagaatc agaaagatca ggtaaaggtc ttagtaaacg caagccattc
    24601 agaataacca atattgtgct tccttcgtgt cctacaactc caagaggtag atttaaaaat
    24661 tgcaggaagt tagaagcaat caacaagaga ataacaagaa tcgaaaaaat aatattttgt
    24721 ttgatgatcc gccgcatttt taaagatagc cgatgactgt agacaaattt atttaagtcg
    24781 cttttcatta agacgatgtc tgcgatatcc atagcggcat ctgtcccttg ccccatagcg
    24841 acacctaaag aagcggctgc taaagcaggc gcatcattga ctccatcacc aaccatcgca
    24901 tttacttggt aaagttcatt ctcttttttg actagttgtg ttttttcttc cggagtacag
    24961 cttgcatagt aatgttcaat ccctacttgt tcagcaatgg cctttgctgt gccagaatgg
    25021 tcgccggtta tcatacttgt atggatgttt tgggatttaa agtaggaaat tacttgttgg
    25081 gtatttgctt ttggaatatc tagcagacct aaaacagcaa ttaactgctg atctttagaa
    25141 agataaatca cggttttgcc ttgattttcc aaacgttcga tcatttccgc aatctctttg
    25201 ctgattatca tcgtctcagg atcatatgcg tgcttgccaa ctttccattg cgcacctaaa
    25261 taggtagttt gtaatccaaa accagtaatt tcttctattg gtaagtattg gatttcttca
    25321 ggtattgtgc aatcgaaata ttgcaaaatc gcatgagcaa gtggatgcgt cgttttttgt
    25381 tccatagcaa caagaagttg ttgtgccaaa tccttttcat caaagaaaaa gctgtcagtt
    25441 acaactggtt ttcctttagt gagcgttcct gttttatcaa aggcaatcgc ttttaattca
    25501 gaaagctttt ctaaatggat accgcctttg attaaaatcc cattttttgc agaatttgaa
    25561 agagcagcta gagaagctgg tgtagcagat gcgacaagcg cacaaggaga agccacaact
    25621 aataatacca ttcctcgata aaagctttca tcccaactcc aacctaggaa aaagtggggg
    25681 agaaggatca ttagaggaac agcaagcaaa atcaatttta cataattatt ttcgattcgt
    25741 tcgataaatg aagctgtttg agttggcgta ttctgcgctt cttctactaa ttggataatc
    25801 ttagcaaaga cagtttcgga actcgttttt gttactcgaa tcgtgatggg atttccttga
    25861 ttcagcgttc ctgcatacat ctcatcgcct aaatattttt ctacaggcac agattcaccg
    25921 cttaatgcag actcatcgat cgttgcctgt gtgctttcta aaaagccatc tatcggaaca
    25981 gctgctcctt tagcgacaaa taccaagtta tccaccttta gttgagaaac atctacttct
    26041 tgcatttgtc cattttcttt taataattgg gctttttgtg gttgaagatt catcaaagca
    26101 gtaatttctt tttgactttt attagttgtg tattcctcta atgcaccgct taaacaaaag
    26161 atgaatgtca acattgctcc ttcaaagtaa ttaccgatca aacaggcacc aatagcagct
    26221 aaagccatca ataaatccac attcagtgtc cgatcattcc acaattcttt caagccttcc
    26281 gatgtttgtt tatatccacc gatgataatg gcactgatat aaaaaagtgg ggtgatggta
    26341 agagagaatc ggtcgaagat aaaaccgaag atcataaata aaaggcagcc gattgttgct
    26401 gcaaaagctt ttttctgatt agtcatcgtt attagacctc cttgtcaaaa caaggatagc
    26461 ataataaaaa tgtaattgat aataataaca gctaaatgag aacgataatg gttatcattt
    26521 ggtgattaag cttgatgttg tttttattat ttataatgat tattattaaa gaaaataaaa
    26581 aaccagaaca tttctcaaat aagagaagtg ttctggtttt ttctacattg caataaacca
    26641 ataaccaatc aaaatgatac caaggataat acgataccag ccaaaagcgg taaagtcgtt
    26701 tttcttcaaa tagcttagta agaatttgat agctaagacg gaaaccacaa aagatacgaa
    26761 tgttcctaca agaagaacaa cagtttcttc cattttgaat gaattccctt cagctaaata
    26821 tttgacgatc ttcaagccgc ttgcgccaaa catgacagga atccctaaga aaaatgaaaa
    26881 ctctgtagcg acaaaacgcg aagcaccaat caagatagat cctaggatcg ttgcgccaga
    26941 acgggatgtt ccgggaacaa gtgccaagac ttggaataac ccaacaagaa gagctgcttg
    27001 gtacgtaaag tcattcagag aagtccaacg aggtgttttg tttttgttgc gtttttcgac
    27061 aataataaag gcaataccgt aaacgatcaa aacaatggca actggtaaga acttgtggaa
    27121 atttgcttct aaccaatcat ccaaaggcaa accaatgatg atggctggta tgcaagcaac
    27181 gatcacttta aaccaaagtg tccacgtatc ttttttttct accgttgatt tacttggaga
    27241 aaatgggttt aacttgtgaa aataaagaac gacgacagcc aagatcgccc ctaattggat
    27301 aacgacattg aacatcgaca taaattgtga actggcatcc agtttgatga attcatctgc
    27361 taggatcaaa tgaccagtac tactgatggg cagccattcc gttattcctt caataatacc
    27421 gagaatcact gctttgataa tatttgcaaa aaacataaat tccttccttc ccaattaaga
    27481 tacatcttat agtataccgt tattttttga gaaacccaat ctgtttttta atattaagaa
    27541 aaacttgaga aataaagcag ataagtacac gaagagaaaa aagaagacaa agccctcgtt
    27601 cagactttgc cttttatgat tctctggtaa aaattaaaat atccccatta tagaaacgag
    27661 tcgcgactat gtgttttccc gttatcgata taagacgtat cattgatgcc gtggatagaa
    27721 aatcgaccgt ttgcataagt gacctttgtt acgctgccat ttaaaagccc agcacgaaca
    27781 ggctgtttag gatcgatcag attaagcaaa aaggcgatcg tcaaaccatg agatacgact
    27841 aaaacatttc caccaccatt tttttcgcag tgatgggcga tatcttcaaa tcctgtccat
    27901 acacggtctc gcaatgtttc ataagtttcc gcccacccag cggtatctaa gttgtataca
    27961 gcattagcaa tttgttcaaa agaaacttct gtggtaaaca tttcatcata tgttttaaaa
    28021 ttcaatacac gagggatcac accccacatt tccatgtcat atccaccttc aagagacccg
    28081 aaacaccatt ctctaatcct tttttcaata aaataaggaa tttcttttcc cttagaatgt
    28141 tctcctagaa taatacgggc agtttcgatt gcacgaccac tgtcactaga ataagcaaat
    28201 gaaaagtcga cattttttaa cccgcgacct aaataatgaa tgccttcttg tccttgcttt
    28261 gtgaggggtg tatcacacca gccttgtact cgttcaattg tattgaacat cgtttttccg
    28321 tgtcgtacga tataaagtgt agtttctgtc attttattct cctcctaagt cgttttaacg
    28381 attgaaaaaa ggatttgtcc ttttttcctg ttcaatcgtc gttgcatcgc catgtcctgg
    28441 atagactgca aattcatcag gtaatacaaa cagctgggtc cgaatactat gaagtaattg
    28501 ttctaaattc cctgtataca gatcggttct accgatactt cctctaaaca gggcgtcgcc
    28561 agaaacgaca aaatcatcaa aaataaagct gacgcttcct atagaatgcc ctggggtagg
    28621 gacgacgcga aaagacatgt ttcctaaagt ataagttttc atttcaaatt catattctgc
    28681 aggcgagaca ataacatcag caatatcatc atgtctaccg agtccagaaa gattcaaaat
    28741 cggatcacct agccattttt gctctagtgg gctgacataa actgggattt gataatacgc
    28801 gcgtaaaggt tccactgcac cgatatgatc gtagtgagta tgggtaagca aaatcgctac
    28861 cggctgttgg tttgtttttt tgatttcttg tttgattcgt tccgcatctt ctccagggtc
    28921 aatgatcaaa agtgcttctt cgttgtagac caaataacag ttttcttcaa tcattcctgt
    28981 ttttaatctt tcgatatgca tgtgcattcc tccttgatag ccaaaacttc tttcatccat
    29041 tagtataacg cattcattgc taggaaacaa aaacaggtac ttatttctct ttacttatct
    29101 gaattatcag actatatgaa aagtatttgg atattctaaa gagaaggaaa gtaagttttc
    29161 atagcatttt aatatgtcct tgttacatta gattcatcca aaaatccaaa ttctatatcc
    29221 tatatccctg cctaatcagc agggtgtttt tttatgtttg gaaagagagc tctttttgct
    29281 aaactaggtt ttttcaggta atcttagaga gaaagaggtg gtggaaatga tcaaacagtc
    29341 aaaaatatct gtgcggcact tagtagaatt tattttgcga cgtggaagta tcgacaatcg
    29401 taaaaaaagt aaccatactg cattagaagg agcgagaatc caccgcaagc tccaaaaaga
    29461 tgcgggagaa aactatgaaa aagaagtctt tttaaaaaca acagttcaat tagatgaata
    29521 ttacctgatc gttgaaggcc gtgcagacgg tattttcaaa agagacggta tttattatat
    29581 agatgaaatc aaaacctccg aaccacgatt tgaagattta gaaccagaac aagtggaact
    29641 tttctttcat caagcaaggg tgtatgccta tatctatagt caagaacatg atttacaaga
    29701 aataaatctg caattgactt acttccagac ggcagaagaa gtgatcacga gaaaggttga
    29761 acatcagaca agagagcagt tagaaacttt ttttaaaaag ctgactgaag attataaaga
    29821 atggcttgtt tttcaagaaa actggcgcac tgtacgcaat gcctctttga tggctttgaa
    29881 attcccccat gatgagtata ggaaaggtca gcgtgaacta gcggtagctg cttataaaac
    29941 gatccgtacg aaacagaaat tgtttgtaga agcacctacc ggaacaggaa aaacaatctc
    30001 tacgttattc cccgctttga aagctgtagg ggaagaagag ggagaaaaga tcttttatct
    30061 aacggctaaa acaataacta gacaagtagc tgaagatgct atgacagcat taaaagatac
    30121 aggagctgaa gtaaaaagtg tcactttgac ggcgaaagac aagatctgct ttttagatga
    30181 gacgacctgt aatccagatc agtgcccata tgccaatggt tattacaatc ggattaatga
    30241 aggtttatgg gatcttttga atcatgagaa tcagatcact cgtgaagtca tcgaaactta
    30301 tgcaagaaag catacacttt gtcctttcga actatcatta gatgtcagta tctggtgtga
    30361 cgtaatcatt ggggattaca attatttatt tgatccaact gtttatttaa gacgtttttt
    30421 tgaagatgaa aaaaacgagg actatctgtt tttgattgac gaagcacata atttggtgaa
    30481 tcgttcccga gagatgtatt cggcagaact atcttatgaa aaaacgaaac gaagtaagga
    30541 agctatacca aaggaattca aaaaacttca tcgccgtttc aacaaactat taaaagaatt
    30601 tgattcgatc cgagaaatag caaaagaaga tcattgggat tatcatcatc aaaaagcacc
    30661 ggctgaatct ttagtaaagg caggttatca attaagcgaa aaaattaaag aatggctggc
    30721 agaatttcct gagcacccat cacaagaaca attgcttcct tattattttg atttgcttca
    30781 ttttttgaag gtcagtgaat tttatgatga tcactacgaa acaacagtcg aaaaaacgta
    30841 tcgcgatctg atagtaaaag agttttgtat tgatccaagc ttgtttcttg agcagtcgtt
    30901 ggataaagga aaaagcagta ttcttttttc tgctagtttt tcaccactct cttattatca
    30961 ggaaacactg gggggacaaa aaagtctggc ttacaaactg ccgagtcctt tcccagaagg
    31021 aaaccagcaa gttttcatcg caaattacct agagacgact tatcgaaaaa gggaacacag
    31081 tctggcaaaa ttagtagaga cgattcatgc ttttgctgat ggaaaggtcg ggaattattt
    31141 cgttttcttt ccttcttatc aatatttaga tcttgcggtg gaagcattca gacagcaata
    31201 tcccgacagc aatgtgctaa tccaagaaac ggacatgaat gaggaagaac gcgaacgatt
    31261 tttaacaaaa tttcaaacaa atcccgctga aacattgatt ggattttgcg tacttggcgg
    31321 gattttttca gaaggaatcg atttgagagg ggaccgattg atcggcagta tgatcgtagg
    31381 tgtaggtttg ccgcagatga atcatgagca ggaactgatc aaatcatatt atgatgagaa
    31441 ggaacacctt ggatttgctt acgcttatca gcttcctgga atgaacaaag tcttgcaagc
    31501 tggtggtcga gtgattcgtg gcatggcgga tcaaggaatc gttgtattgg cagatcaacg
    31561 attttctagt ttttcttatc gacgtttatt cccacaacat tggcaaacag caagagaagt
    31621 acgaagtgtt ggggaattgg aagaaggaat acagcgtttt tggttatcaa aagaagcagt
    31681 aaatcaaaaa aacgaggaat gacagacgtc agcctcgttt tttgattaac gatagttagt
    31741 gaattgcaac tcgattggta agtcaagttt acgtaataaa gcaatcacag cttgtagatc
    31801 atcacggttc ttacctgtca ctctaatctg atcttcttgg atttgtgctt ttactttcag
    31861 tttttcattt ttgatagctg tagtgatttt cttcgcgttt tcacgatcga ttccgttgac
    31921 caaatcggca gtttgtcgag ctttgccacc tagagcgtgt tctgattcgg aaaaatggat
    31981 atttttgatt ggcacatttc ttttgatcag cttaccaaat aatacatctt tgatctgttc
    32041 gattttgaaa tcatcatcgc tcaatacaac tagattgctg ttctctaatt tgatttctac
    32101 tgtagaatcc ttgaagtcaa agcgattgga caattctttt gttgtttgct gaatcgcatt
    32161 ttttacttct tctgtattga cttctgatac aatgtcgaag ctagcatctt ttgccatagg
    32221 aaccctcctg atatacatat aaatatttac catacatcta tcttatcata ggattagggc
    32281 agattcttac ctgtttgcta aaattcttct catttgagtt cgaagtattt tattttaata
    32341 gataaactgt tattcttaca tttggaatac caaagtaggg gagaggtgct tgtgagtaaa
    32401 attatgactg cgtatcataa aaatgaaaga ttaaagaaaa tagtggttat tttgataacc
    32461 ggattgaccg ctgcggtcgc tttaaattat tttttgattc cagccaatgt cttttctgca
    32521 gggatgaatg ggatcgctca aatcatttcc tcgcttttat atgaatggtt tcatatcaag
    32581 atagacacag gaacatttat ttttcttttg aatatccctg tgtttgtatt agggtttctc
    32641 aaagtaggaa aacgagcaac gatccttagt tttgtgaatg ttgtatgtgt atcggtgatg
    32701 acgatgctgc ttccagtcgg acagatcact gataatattt tgatgaatgc cctagtaggc
    32761 ggtgttttat taggtcttgg cgtaggatta tctttaaaaa tgggctttac aactggtggg
    32821 atggatatca tctctcttgt actttcacaa acaactggaa aaacagtcgg aaactacatg
    32881 ctgattctaa atggtatcat tgtagtcatc gcaggcttct tctttaactg ggaaagtgcc
    32941 ttgtatacga ttatttccat ttatgcaatg actcaagtaa ttgatgccat ccacacgagt
    33001 catcaaaaag taacagcaat gatcgttacc gtcaagccag aagaggtaac agaagaacta
    33061 gcaaaacgga tggtacgtgg aatgacatta ctgccgtctg tcggtggcta ttcaaaaaaa
    33121 caagggaaca tgatcatgat ggtcatcacc cgctacgaaa tgtatgatct tgatcagatc
    33181 gttcatgaaa tcgacgacga tgcatttatc aatattttac caactcattc agtatttggc
    33241 cggtttgcaa atgaaaacga acaaaaaatg tatcgaacaa ctggcgtatt gccagaaata
    33301 aaaaatcgtt cacaaaaaaa atgaggaatc aggtttattt tagaataaat ttaatctatt
    33361 acatctaata agaacatttt tataaattta aacgagaatc ttacttctaa attctcattt
    33421 acaagcagct aaaaaaagag tatgataaat aaaaatcgtg ttaaggagtg tcgtacgatg
    33481 agcgaagcaa accaagaacg gtctggacaa gaaatagaac taatcaaaga acgtattctt
    33541 gctgccttgg aaatggtgat tgatcctgaa ttaggaatcg acatcgtcaa tttaggcttg
    33601 atttatgata tagaattcaa tcctgaaaat ggagaaacag tcattaagat gactctaaca
    33661 actatgggct gtccattagc agatatttta acagaatcta tccatgacgc attaaaagaa
    33721 gtgccagaag taacgaaagc agaagtcaaa ctagtctggt atccagcatg gacgacagat
    33781 aagatgagcc gttatgcacg gattgcgttg gggatacgat agaaatcttg gctgactagc
    33841 tatgagaagc aaataggaaa aacaaaaaag ccttttatcc tttctagaag aaaagagata
    33901 aaaggtttct ttgtttataa aattttgtct gctatattat aagttaatta aataaatgtg
    33961 ttctattgat aaaggacatt caaatccctt tccttttgga atccttcttt tttacatcag
    34021 tttgattttc gtatagtatt ctttctcatc ttcacgataa atcgcagccc catttttttg
    34081 ttgtgcttta agactgccaa gatttttttt atttacagac aaaagtgcct catcaatgcc
    34141 gatttttttt gcctcttgta gtactaatgc taatccttgt gtggcatatc cttttttacg
    34201 ataattaggg gagatgccgt aaccaatatg gccagcgccg tttcttaatg tttcgttcag
    34261 tttatggcgc aaattaaaaa ttcctacata ctcatgttca tcactttcta aaataaagac
    34321 ggttgcggga acgtaactct caggaagatc gattcctaaa gacgtttttt cgcatttttt
    34381 tacataatct aaaaattcag caaatgtata gccataagca ttgttgataa atccactctc
    34441 atttggtcca aagctagttt gtagctgata agctttcttt aaatctgctt cccatagttt
    34501 gatcagtttc atcgtattca cctctttaag taaccttatt catttgtttt ctactatagc
    34561 actattgttg taaatagcca tgcctatttt tatttattgt gtcatatcgt attaagaatc
    34621 atcgaacaat acaaatatga attacaataa aaaaattatt tgaagttcgt tcattcgtta
    34681 cttatgatct ctagaagaaa gaaaacgtga attacttcag cgtctagaat tccagaagga
    34741 tagtaatcaa cagaagaaat aatcaatatc caaatgaaag aagatagctg aatcgagaaa
    34801 aagaggaaaa aagaaatgaa gaaaaaaata aaatagttac taatcccaga gtcgttcgtt
    34861 taacattgaa atcgatcata tggataaaac agattttttc accaaccctc aatagatgat
    34921 ggagttgctc ggatgaatta tttgtgtaga tggcataagg gagggcgtac agaaatgaaa
    34981 aagaacaaaa atatgttctt gacaaaaaac acatgtaatc ctaaactaaa agatgtgatg
    35041 cggctatggc ggaattggca gacgcgctag cttcaggcgc tagtgagggt gacctcgtgg
    35101 aagttcgagt cttcttagcc gcataatttg tttttttata aagagaaggt acacctggag
    35161 cgatgagaaa gaaaataaca tattatctaa aagaatatag gaaaaatttt aaacaacgga
    35221 accagattca aataaatcaa aaaaaatttg tgtgaaggtg ggacagttta aaacctaata
    35281 gattgattca aatagtagat ttatagattc tgatacaaga aatactggca aacgatagtt
    35341 aaggaataaa gactaaactt aaactcaaaa agaggtcatg cgaaagaata aatcgcatga
    35401 cctcttacta tattttgaat gtgtttaaac gttcaaataa tgaatcttag taccaaccgt
    35461 ttgcttccca gaatgctttc gccgcttccc aagaaccata acgtgaagta acatattgtt
    35521 ctgctactct ttcttggttt gcagcagagt agtcaccatt taagtaagaa gaatctaatt
    35581 ggtaacggcc gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat tctttttgtg
    35641 cgatccattc ttttgcagag cttgtacttg ctggagtagc agcagtttct gttacagcag
    35701 gagcaggttg tgtttcagca acaggcgctg cttgtgtttc cgtttgaacc gtttcttgta
    35761 caacaggttg ttcagcttga acaggttcag cttggactgg tgcttcttga acggctgctt
    35821 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
    35881 a
//
LOCUS       DAZUMO010000025        34687 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000025 DAZUMO010000000
VERSION     DAZUMO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.025"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG8ER_001244"
     CDS             591..1280
                     /locus_tag="ACG8ER_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5049839.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG8ER_001245"
     CDS             1267..2475
                     /locus_tag="ACG8ER_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5049840.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG8ER_001246"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG8ER_001246"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS5049841.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8ER_001247"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8ER_001247"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS5049842.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG8ER_001248"
     CDS             complement(6578..7273)
                     /locus_tag="ACG8ER_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS5049843.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8ER_001249"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8ER_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS5049844.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG8ER_001250"
     CDS             complement(8472..8663)
                     /locus_tag="ACG8ER_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS5049845.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG8ER_001251"
     CDS             complement(8684..9574)
                     /locus_tag="ACG8ER_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS5049846.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG8ER_001252"
     CDS             complement(9561..10328)
                     /locus_tag="ACG8ER_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5049847.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8ER_001253"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8ER_001253"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS5049848.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8ER_001254"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8ER_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS5049849.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8ER_001255"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8ER_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS5049850.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG8ER_001256"
     CDS             14899..15282
                     /locus_tag="ACG8ER_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049851.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8ER_001257"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8ER_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS5049852.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG8ER_001258"
     CDS             complement(16226..17044)
                     /locus_tag="ACG8ER_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS5049853.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8ER_001259"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8ER_001259"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5049854.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8ER_001260"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8ER_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS5049855.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8ER_001261"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8ER_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS5049856.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG8ER_001262"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG8ER_001262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS5049857.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG8ER_001263"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG8ER_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS5049858.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACG8ER_001264"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACG8ER_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS5049859.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG8ER_001265"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG8ER_001265"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS5049860.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG8ER_001266"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG8ER_001266"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS5049861.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG8ER_001267"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG8ER_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS5049862.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACG8ER_001268"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACG8ER_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5049863.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG8ER_001269"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG8ER_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS5049864.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACG8ER_001270"
     CDS             28352..30325
                     /locus_tag="ACG8ER_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS5049865.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACG8ER_001271"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACG8ER_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS5049866.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG8ER_001272"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG8ER_001272"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS5049867.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACG8ER_001273"
     CDS             33204..34496
                     /locus_tag="ACG8ER_001273"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS5049868.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10569 a         6743 c         6858 g        10517 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaa
//
LOCUS       DAZUMO010000026        34619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000026 DAZUMO010000000
VERSION     DAZUMO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.026"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..911)
                     /locus_tag="ACG8ER_001274"
     CDS             complement(63..911)
                     /locus_tag="ACG8ER_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5049869.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="ACG8ER_001275"
     CDS             complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="ACG8ER_001275"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS5049870.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3412..3591)
                     /locus_tag="ACG8ER_001276"
     CDS             complement(3412..3591)
                     /locus_tag="ACG8ER_001276"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS5049871.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3638..5461)
                     /locus_tag="ACG8ER_001277"
     CDS             complement(3638..5461)
                     /locus_tag="ACG8ER_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5049872.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5739..8018
                     /gene="helD"
                     /locus_tag="ACG8ER_001278"
     CDS             5739..8018
                     /gene="helD"
                     /locus_tag="ACG8ER_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS5049873.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8111..8758)
                     /locus_tag="ACG8ER_001279"
     CDS             complement(8111..8758)
                     /locus_tag="ACG8ER_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049874.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8937..9656)
                     /locus_tag="ACG8ER_001280"
     CDS             complement(8937..9656)
                     /locus_tag="ACG8ER_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5049875.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9787..10938)
                     /locus_tag="ACG8ER_001281"
     CDS             complement(9787..10938)
                     /locus_tag="ACG8ER_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS5049876.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11155..11895)
                     /locus_tag="ACG8ER_001282"
     CDS             complement(11155..11895)
                     /locus_tag="ACG8ER_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5049877.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="ACG8ER_001283"
     CDS             complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="ACG8ER_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS5049878.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="ACG8ER_001284"
     CDS             complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="ACG8ER_001284"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS5049879.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12856..13506)
                     /locus_tag="ACG8ER_001285"
     CDS             complement(12856..13506)
                     /locus_tag="ACG8ER_001285"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS5049880.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="ACG8ER_001286"
     CDS             complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="ACG8ER_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS5049881.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="ACG8ER_001287"
     CDS             complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="ACG8ER_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS5049882.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14949..15476)
                     /locus_tag="ACG8ER_001288"
     CDS             complement(14949..15476)
                     /locus_tag="ACG8ER_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS5049883.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15656..16441)
                     /locus_tag="ACG8ER_001289"
     CDS             complement(15656..16441)
                     /locus_tag="ACG8ER_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS5049884.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16434..17303)
                     /gene="accD"
                     /locus_tag="ACG8ER_001290"
     CDS             complement(16434..17303)
                     /gene="accD"
                     /locus_tag="ACG8ER_001290"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS5049885.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17304..18683)
                     /locus_tag="ACG8ER_001291"
     CDS             complement(17304..18683)
                     /locus_tag="ACG8ER_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS5049886.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="ACG8ER_001292"
     CDS             complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="ACG8ER_001292"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5049887.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19135..19611)
                     /gene="accB"
                     /locus_tag="ACG8ER_001293"
     CDS             complement(19135..19611)
                     /gene="accB"
                     /locus_tag="ACG8ER_001293"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS5049888.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="ACG8ER_001294"
     CDS             complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="ACG8ER_001294"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS5049889.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="ACG8ER_001295"
     CDS             complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="ACG8ER_001295"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS5049890.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="ACG8ER_001296"
     CDS             complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="ACG8ER_001296"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5049891.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22641..22865)
                     /locus_tag="ACG8ER_001297"
     CDS             complement(22641..22865)
                     /locus_tag="ACG8ER_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5049892.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22918..23880)
                     /locus_tag="ACG8ER_001298"
     CDS             complement(22918..23880)
                     /locus_tag="ACG8ER_001298"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS5049893.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23890..24330)
                     /locus_tag="ACG8ER_001299"
     CDS             complement(23890..24330)
                     /locus_tag="ACG8ER_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5049894.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24636..25601
                     /locus_tag="ACG8ER_001300"
     CDS             24636..25601
                     /locus_tag="ACG8ER_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5049895.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25658..25888)
                     /locus_tag="ACG8ER_001301"
     CDS             complement(25658..25888)
                     /locus_tag="ACG8ER_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS5049896.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25974..26861)
                     /locus_tag="ACG8ER_001302"
     CDS             complement(25974..26861)
                     /locus_tag="ACG8ER_001302"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5049897.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26950..28173)
                     /locus_tag="ACG8ER_001303"
     CDS             complement(26950..28173)
                     /locus_tag="ACG8ER_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5049898.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28190..29236)
                     /locus_tag="ACG8ER_001304"
     CDS             complement(28190..29236)
                     /locus_tag="ACG8ER_001304"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5049899.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29400..31127)
                     /gene="ptsP"
                     /locus_tag="ACG8ER_001305"
     CDS             complement(29400..31127)
                     /gene="ptsP"
                     /locus_tag="ACG8ER_001305"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS5049900.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRGLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31127..31393)
                     /locus_tag="ACG8ER_001306"
     CDS             complement(31127..31393)
                     /locus_tag="ACG8ER_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS5049901.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31872..34106
                     /locus_tag="ACG8ER_001307"
     CDS             31872..34106
                     /locus_tag="ACG8ER_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5049902.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34254..34574
                     /locus_tag="ACG8ER_001308"
     CDS             34254..34574
                     /locus_tag="ACG8ER_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5049903.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9778 a         7487 c         6043 g        11311 t
ORIGIN      
        1 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
       61 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
      121 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
      181 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
      241 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
      301 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
      361 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
      421 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
      481 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
      541 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
      601 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
      661 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
      721 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
      781 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
      841 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
      901 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
      961 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     1021 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     1081 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     1141 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     1201 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     1261 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     1321 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     1381 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     1441 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     1501 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     1561 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     1621 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     1681 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     1741 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     1801 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     1861 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     1921 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     1981 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     2041 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     2101 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     2161 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     2221 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     2281 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     2341 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     2401 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     2461 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     2521 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     2581 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     2641 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     2701 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     2761 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     2821 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     2881 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     2941 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     3001 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     3061 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     3121 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     3181 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     3241 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     3301 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     3361 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     3421 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     3481 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     3541 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     3601 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     3661 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     3721 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     3781 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     3841 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     3901 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     3961 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     4021 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     4081 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     4141 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     4201 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     4261 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     4321 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     4381 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     4441 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     4501 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     4561 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     4621 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     4681 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     4741 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     4801 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     4861 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     4921 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     4981 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     5041 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     5101 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     5161 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     5221 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     5281 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     5341 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     5401 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     5461 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     5521 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     5581 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     5641 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     5701 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     5761 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     5821 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     5881 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     5941 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     6001 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     6061 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     6121 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     6181 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     6241 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     6301 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     6361 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     6421 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     6481 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     6541 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     6601 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     6661 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     6721 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     6781 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     6841 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     6901 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     6961 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     7021 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     7081 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     7141 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     7201 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     7261 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     7321 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     7381 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     7441 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
     7501 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
     7561 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
     7621 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
     7681 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
     7741 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
     7801 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
     7861 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
     7921 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
     7981 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
     8041 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
     8101 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
     8161 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
     8221 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
     8281 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
     8341 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
     8401 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
     8461 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
     8521 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
     8581 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
     8641 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
     8701 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
     8761 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
     8821 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
     8881 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
     8941 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
     9001 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
     9061 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
     9121 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
     9181 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
     9241 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
     9301 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
     9361 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
     9421 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
     9481 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
     9541 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
     9601 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
     9661 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
     9721 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
     9781 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
     9841 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
     9901 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
     9961 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    10021 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    10081 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    10141 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    10201 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    10261 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    10321 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    10381 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    10441 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    10501 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    10561 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    10621 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    10681 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    10741 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    10801 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    10861 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    10921 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    10981 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    11041 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    11101 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    11161 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    11221 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    11281 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    11341 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    11401 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    11461 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    11521 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    11581 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    11641 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    11701 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    11761 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    11821 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    11881 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    11941 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    12001 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    12061 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    12121 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    12181 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    12241 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    12301 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    12361 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    12421 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    12481 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    12541 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    12601 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    12661 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    12721 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    12781 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    12841 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    12901 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    12961 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    13021 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    13081 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    13141 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    13201 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    13261 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    13321 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    13381 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    13441 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    13501 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    13561 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    13621 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    13681 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    13741 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    13801 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    13861 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    13921 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    13981 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    14041 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    14101 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    14161 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    14221 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    14281 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    14341 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    14401 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    14461 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    14521 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    14581 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    14641 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    14701 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    14761 caaaaagtcg tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa
    14821 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    14881 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    14941 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    15001 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    15061 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    15121 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    15181 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    15241 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    15301 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    15361 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    15421 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    15481 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    15541 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    15601 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    15661 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    15721 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    15781 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    15841 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    15901 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    15961 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    16021 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    16081 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    16141 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    16201 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    16261 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    16321 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    16381 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    16441 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    16501 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    16561 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    16621 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    16681 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    16741 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    16801 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    16861 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    16921 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    16981 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    17041 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    17101 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    17161 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    17221 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    17281 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    17341 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    17401 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    17461 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    17521 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    17581 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    17641 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    17701 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    17761 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    17821 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    17881 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    17941 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    18001 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    18061 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    18121 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    18181 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    18241 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    18301 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    18361 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    18421 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    18481 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    18541 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    18601 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    18661 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    18721 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    18781 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    18841 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    18901 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    18961 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    19021 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    19081 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    19141 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    19201 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    19261 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    19321 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    19381 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac
    19441 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    19501 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    19561 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    19621 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    19681 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    19741 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    19801 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    19861 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    19921 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    19981 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    20041 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    20101 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    20161 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    20221 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    20281 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    20341 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    20401 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    20461 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    20521 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    20581 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    20641 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    20701 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    20761 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    20821 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    20881 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    20941 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    21001 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    21061 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    21121 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    21181 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    21241 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    21301 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    21361 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    21421 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    21481 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    21541 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    21601 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    21661 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    21721 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    21781 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    21841 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    21901 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    21961 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    22021 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    22081 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    22141 tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt
    22201 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    22261 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    22321 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    22381 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    22441 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    22501 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    22561 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    22621 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    22681 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    22741 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    22801 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    22861 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    22921 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    22981 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    23041 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    23101 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    23161 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    23221 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    23281 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    23341 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    23401 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    23461 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    23521 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    23581 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    23641 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    23701 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    23761 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    23821 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    23881 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    23941 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    24001 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    24061 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    24121 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    24181 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    24241 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    24301 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    24361 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc
    24421 atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca
    24481 gcatttataa tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt
    24541 attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt
    24601 atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc
    24661 tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg
    24721 gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt
    24781 caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca
    24841 cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata
    24901 accgacaaat gaattatttc agtaattata attatgtaca tattaccaat aagggatttg
    24961 tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc
    25021 cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt
    25081 tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa
    25141 aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa
    25201 gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata
    25261 tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac
    25321 catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg
    25381 tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct
    25441 attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg
    25501 atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg
    25561 aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt
    25621 aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt
    25681 ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta
    25741 attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt
    25801 caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta
    25861 tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat
    25921 tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat
    25981 tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca
    26041 ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc
    26101 aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt
    26161 ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa
    26221 gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg
    26281 tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc
    26341 tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta
    26401 ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg
    26461 atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca
    26521 tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta
    26581 gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag
    26641 tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct
    26701 gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt
    26761 gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca
    26821 gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa
    26881 tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat
    26941 gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc
    27001 ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat
    27061 catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg
    27121 atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt
    27181 ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc
    27241 cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga
    27301 agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc
    27361 cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa
    27421 ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat
    27481 gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa
    27541 agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc
    27601 ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg
    27661 aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac
    27721 tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat
    27781 cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat
    27841 cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt
    27901 atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc
    27961 tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac
    28021 atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc
    28081 atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg
    28141 aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga
    28201 cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat
    28261 ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt
    28321 gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt
    28381 ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac actggaatat
    28441 ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct
    28501 cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc
    28561 cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga
    28621 tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca
    28681 tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac
    28741 aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca
    28801 ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac
    28861 ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg
    28921 ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga
    28981 taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt
    29041 ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga
    29101 tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt
    29161 tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat
    29221 ataataatac cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac
    29281 tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa
    29341 aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt
    29401 tattttacat attcatgaac aagttcaacg acttcttcca tcgtatcgca ttctttcaat
    29461 gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa accacgcgtt
    29521 ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga
    29581 acagctgttt gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt
    29641 gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag
    29701 taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta
    29761 cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga
    29821 atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat
    29881 ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc
    29941 atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt
    30001 gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag
    30061 aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc
    30121 attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca
    30181 tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac
    30241 agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg
    30301 tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt
    30361 tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt
    30421 tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct
    30481 tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt
    30541 gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa
    30601 ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct
    30661 tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta
    30721 gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg
    30781 aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg
    30841 tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg
    30901 gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc
    30961 tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta
    31021 tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca
    31081 acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc
    31141 cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg
    31201 cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt
    31261 tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg
    31321 tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt
    31381 ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt
    31441 cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca
    31501 ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt
    31561 ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc
    31621 ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat
    31681 atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga
    31741 atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat
    31801 tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac
    31861 gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg
    31921 ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca
    31981 aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca
    32041 gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc
    32101 aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag
    32161 gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag
    32221 gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa
    32281 atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag
    32341 ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca
    32401 aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat
    32461 ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat
    32521 tcatagatga agttcacgaa atagtcggtg ctggttctgc tggtgatgga aatatggatg
    32581 ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta
    32641 ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg
    32701 tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac
    32761 gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt
    32821 tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg
    32881 aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa
    32941 aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag
    33001 cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg
    33061 aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag
    33121 gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg
    33181 acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc
    33241 gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg
    33301 gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt
    33361 cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta
    33421 aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa
    33481 aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag
    33541 atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa
    33601 gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag
    33661 tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc
    33721 aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca
    33781 gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc
    33841 aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag
    33901 ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac
    33961 aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag
    34021 caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag
    34081 aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg
    34141 agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag
    34201 aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat
    34261 tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg
    34321 cccatctagt caaggtctac acagccggaa ataccactat tgtctacact gaagcccctc
    34381 aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag
    34441 atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca
    34501 aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta
    34561 cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaa
//
LOCUS       DAZUMO010000027        32169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000027 DAZUMO010000000
VERSION     DAZUMO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.027"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG8ER_001309"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG8ER_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS5049904.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG8ER_001310"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG8ER_001310"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS5049905.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="ACG8ER_001311"
     CDS             1947..2645
                     /locus_tag="ACG8ER_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5049906.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="ACG8ER_001312"
     CDS             complement(2813..5452)
                     /locus_tag="ACG8ER_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5049907.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6071..7102)
                     /gene="queA"
                     /locus_tag="ACG8ER_001313"
     CDS             complement(6071..7102)
                     /gene="queA"
                     /locus_tag="ACG8ER_001313"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS5049908.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7295..8470
                     /locus_tag="ACG8ER_001314"
     CDS             7295..8470
                     /locus_tag="ACG8ER_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5049909.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8474..9115
                     /locus_tag="ACG8ER_001315"
     CDS             8474..9115
                     /locus_tag="ACG8ER_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049910.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9112..10029
                     /locus_tag="ACG8ER_001316"
     CDS             9112..10029
                     /locus_tag="ACG8ER_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5049911.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10026..10697
                     /locus_tag="ACG8ER_001317"
     CDS             10026..10697
                     /locus_tag="ACG8ER_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5049912.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11238..11726)
                     /locus_tag="ACG8ER_001318"
     CDS             complement(11238..11726)
                     /locus_tag="ACG8ER_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS5049913.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11927..12193)
                     /locus_tag="ACG8ER_001319"
     CDS             complement(11927..12193)
                     /locus_tag="ACG8ER_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5049914.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12193..12456)
                     /locus_tag="ACG8ER_001320"
     CDS             complement(12193..12456)
                     /locus_tag="ACG8ER_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5049915.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12977..13117
                     /locus_tag="ACG8ER_001321"
                     /pseudo
     CDS             12977..13117
                     /locus_tag="ACG8ER_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13259..15082)
                     /locus_tag="ACG8ER_001322"
     CDS             complement(13259..15082)
                     /locus_tag="ACG8ER_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5049916.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15680..18385)
                     /locus_tag="ACG8ER_001323"
     CDS             complement(15680..18385)
                     /locus_tag="ACG8ER_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS5049917.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18691..19638)
                     /locus_tag="ACG8ER_001324"
     CDS             complement(18691..19638)
                     /locus_tag="ACG8ER_001324"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS5049918.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19828..20097
                     /gene="rpsN"
                     /locus_tag="ACG8ER_001325"
     CDS             19828..20097
                     /gene="rpsN"
                     /locus_tag="ACG8ER_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5049919.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20273..20635)
                     /locus_tag="ACG8ER_001326"
     CDS             complement(20273..20635)
                     /locus_tag="ACG8ER_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5049920.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20646..21767)
                     /gene="alr"
                     /locus_tag="ACG8ER_001327"
     CDS             complement(20646..21767)
                     /gene="alr"
                     /locus_tag="ACG8ER_001327"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS5049921.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21783..22133)
                     /gene="acpS"
                     /locus_tag="ACG8ER_001328"
     CDS             complement(21783..22133)
                     /gene="acpS"
                     /locus_tag="ACG8ER_001328"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS5049922.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22263..23774)
                     /locus_tag="ACG8ER_001329"
     CDS             complement(22263..23774)
                     /locus_tag="ACG8ER_001329"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5049923.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24096..25448)
                     /gene="murF"
                     /locus_tag="ACG8ER_001330"
     CDS             complement(24096..25448)
                     /gene="murF"
                     /locus_tag="ACG8ER_001330"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS5049924.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25601..26677)
                     /locus_tag="ACG8ER_001331"
     CDS             complement(25601..26677)
                     /locus_tag="ACG8ER_001331"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS5049925.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26873..28618
                     /locus_tag="ACG8ER_001332"
     CDS             26873..28618
                     /locus_tag="ACG8ER_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS5049926.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28898..29335)
                     /locus_tag="ACG8ER_001333"
     CDS             complement(28898..29335)
                     /locus_tag="ACG8ER_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS5049927.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29319..30692)
                     /locus_tag="ACG8ER_001334"
     CDS             complement(29319..30692)
                     /locus_tag="ACG8ER_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049928.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31014..31502)
                     /locus_tag="ACG8ER_001335"
     CDS             complement(31014..31502)
                     /locus_tag="ACG8ER_001335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5049929.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31524..32126)
                     /locus_tag="ACG8ER_001336"
     CDS             complement(31524..32126)
                     /locus_tag="ACG8ER_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5049930.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9195 a         6879 c         5940 g        10155 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
     6061 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
     6121 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
     6181 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
     6241 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
     6301 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
     6361 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
     6421 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
     6481 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
     6541 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
     6601 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
     6661 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
     6721 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
     6781 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
     6841 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
     6901 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
     6961 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
     7021 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
     7081 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
     7141 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
     7201 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
     7261 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
     7321 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
     7381 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
     7441 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
     7501 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
     7561 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
     7621 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
     7681 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
     7741 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
     7801 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
     7861 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
     7921 gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa
     7981 cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac
     8041 aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca
     8101 agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc
     8161 gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc
     8221 cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg
     8281 tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca
     8341 gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat
     8401 ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa
     8461 ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg
     8521 ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc
     8581 gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag
     8641 tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt
     8701 aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa
     8761 tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg
     8821 gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat
     8881 ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat
     8941 cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct
     9001 cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg
     9061 tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt
     9121 atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct
     9181 ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa
     9241 gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc
     9301 gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat
     9361 gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct
     9421 cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac
     9481 ggtcaaacgt ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa
     9541 gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa
     9601 ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc
     9661 caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat
     9721 gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc
     9781 gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct
     9841 gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat
     9901 aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat
     9961 aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa
    10021 gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg
    10081 aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg
    10141 tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat
    10201 tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga
    10261 tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt
    10321 tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa
    10381 acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag
    10441 agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca
    10501 tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc
    10561 gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat
    10621 taatggcgat catcacggac tggcttttag gtattttgga aagacggcta gatccagcca
    10681 gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc
    10741 gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc
    10801 ttatgggcaa agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata
    10861 ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat
    10921 tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac
    10981 tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt
    11041 taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt
    11101 gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc
    11161 cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta
    11221 aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga
    11281 tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag
    11341 acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg
    11401 gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc
    11461 gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct
    11521 tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg
    11581 tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc
    11641 ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct
    11701 ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa
    11761 cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa
    11821 agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt
    11881 ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg
    11941 tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat
    12001 gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag
    12061 actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct
    12121 ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg
    12181 tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc
    12241 gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc
    12301 ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact
    12361 aataatctcg atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt
    12421 acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat
    12481 aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca
    12541 aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac
    12601 gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg
    12661 tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca
    12721 cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac
    12781 gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa
    12841 aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta
    12901 gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg
    12961 gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt
    13021 aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta
    13081 tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca
    13141 tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa
    13201 cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt
    13261 atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca
    13321 atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa
    13381 attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat
    13441 ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac
    13501 ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt
    13561 cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg
    13621 caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat
    13681 catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat
    13741 ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc
    13801 ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat
    13861 tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg
    13921 tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg
    13981 ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat
    14041 tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg
    14101 ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa
    14161 tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac
    14221 caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa
    14281 atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg
    14341 cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag
    14401 ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca
    14461 cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata
    14521 aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga
    14581 acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca
    14641 cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac
    14701 ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa
    14761 tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag
    14821 gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa
    14881 aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa
    14941 cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag
    15001 cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta
    15061 acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt
    15121 tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca
    15181 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
    15241 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
    15301 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
    15361 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
    15421 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa
    15481 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
    15541 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    15601 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
    15661 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
    15721 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
    15781 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
    15841 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
    15901 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
    15961 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
    16021 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
    16081 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
    16141 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
    16201 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
    16261 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
    16321 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
    16381 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
    16441 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
    16501 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
    16561 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
    16621 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
    16681 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
    16741 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
    16801 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
    16861 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
    16921 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
    16981 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
    17041 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
    17101 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
    17161 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
    17221 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
    17281 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
    17341 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
    17401 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
    17461 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
    17521 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
    17581 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
    17641 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
    17701 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
    17761 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
    17821 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
    17881 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
    17941 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
    18001 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
    18061 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
    18121 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
    18181 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
    18241 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
    18301 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
    18361 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
    18421 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
    18481 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
    18541 agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta
    18601 ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc
    18661 agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc
    18721 agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga
    18781 cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc
    18841 aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt
    18901 tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt
    18961 catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc
    19021 tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc
    19081 tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc
    19141 cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat
    19201 tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc
    19261 tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt ttgctacttt
    19321 tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg
    19381 atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc
    19441 attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa
    19501 ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc
    19561 gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt
    19621 aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt
    19681 tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc
    19741 ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta
    19801 ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa
    19861 aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa
    19921 gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt
    19981 gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa
    20041 ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc
    20101 aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa
    20161 gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac
    20221 gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag
    20281 taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca
    20341 taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga
    20401 tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa
    20461 tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg
    20521 ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct
    20581 ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat
    20641 cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag
    20701 tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg
    20761 ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga
    20821 tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc
    20881 tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct
    20941 tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact
    21001 tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg
    21061 tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt
    21121 tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga
    21181 agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc
    21241 tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg
    21301 atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca
    21361 gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca
    21421 gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct
    21481 acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct
    21541 tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca
    21601 actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca
    21661 aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct
    21721 tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct
    21781 ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc
    21841 catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa
    21901 atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc
    21961 tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg
    22021 aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc
    22081 agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt
    22141 tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa
    22201 ataagtccac aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat
    22261 gtttaatcgt tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg
    22321 ccatcttttt ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa
    22381 ttaccgccgt cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat
    22441 ccttttttct gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct
    22501 ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct
    22561 aataatttat ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg
    22621 atttgttcaa ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt
    22681 aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt
    22741 ggtgtaacga aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg
    22801 tgaacgtagc tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa
    22861 atatctaatc cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta
    22921 aatgaacgca atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca
    22981 cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg
    23041 aagacgattg ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt
    23101 tcgtattctt ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct
    23161 ttgatcttca catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc
    23221 ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt
    23281 tcgatatctt ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt
    23341 tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta
    23401 cccacaacga tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg
    23461 ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt
    23521 gtttggatag ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct
    23581 ggatcgattt tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt
    23641 gcttgtccaa taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc
    23701 gttgcttctt caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt
    23761 tctttaaatt tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc
    23821 taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt
    23881 ttttatccat tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat
    23941 tagaaaagcc aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa
    24001 gttgtttcta tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta
    24061 acatagtttt tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct
    24121 accacttcaa ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca
    24181 agattttgtt tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt
    24241 agttgggggt atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg
    24301 aaaatcatgg cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa
    24361 gactctttcc ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc
    24421 agatttttaa aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg
    24481 ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa
    24541 acatgggcga gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta
    24601 tacgcaatca aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct
    24661 ccttctacta caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg
    24721 ccatttccaa taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc
    24781 aacggttcat ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc
    24841 attttggctt tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc
    24901 attgtgattg ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg
    24961 tgatccatgc ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg
    25021 taaggaagac caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca
    25081 gacaagacag acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca
    25141 acgacttctg cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa
    25201 ggatctgcta caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa
    25261 aatgaagcag atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta
    25321 ccagcaagag gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg
    25381 gtaatctttg tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt
    25441 agattcattg agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg
    25501 gttcgcatga ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa
    25561 cgcggttacg caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa
    25621 aagattgacg ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac
    25681 cgtatttcaa gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg
    25741 gcatagagtt taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc
    25801 ggctcaatcc gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt
    25861 gataaacttc ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt
    25921 ttgcttcata atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa
    25981 cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga
    26041 ttgctcgaga atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt
    26101 ctgcttttgt aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa
    26161 gcaaagaacc ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa
    26221 gtactggtac ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga
    26281 ttttatccat ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg
    26341 tctctaagaa gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa
    26401 cgatggctcc ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct
    26461 ctgtctgtcc acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg
    26521 ttagtaatgg acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt
    26581 aataattata ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct
    26641 ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa
    26701 atcgttcagt gatttactct catattttag cacaaaacaa aaataaacga ataacttatt
    26761 tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat
    26821 tatttgtccc gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca
    26881 aacaaatcca cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt
    26941 gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg
    27001 ccccaccttc atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc
    27061 cttcactcaa acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt
    27121 caaaaagacg atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc
    27181 aaaatcgcct tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg
    27241 tgacgcggta gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct
    27301 tcaactattt gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga
    27361 tctaaaaggg gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa
    27421 agaaagtcaa aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag
    27481 ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg
    27541 taaaaacttg aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat
    27601 tcaaaacaag cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca
    27661 ggtgtttgat gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt
    27721 actaacaaaa gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga
    27781 gttcggttcc tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac
    27841 catcaactac atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact
    27901 cacacaagcc gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct
    27961 tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag
    28021 ttttcttgaa cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac
    28081 acatgtcgtg acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt
    28141 gctgaaagca tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct
    28201 catggaagtc gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga
    28261 tacattgaaa caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc
    28321 tcaacagctc caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga
    28381 attgaaaaag caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt
    28441 cggtatgatt ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt
    28501 agaaaacgca gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt
    28561 cattattttt ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa
    28621 aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt
    28681 tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag
    28741 aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca
    28801 tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat
    28861 accttcgtta cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg
    28921 cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc
    28981 cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt
    29041 cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag
    29101 ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat
    29161 aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt
    29221 tggtcgtata atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta
    29281 attttatgac atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt
    29341 ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg
    29401 atccattttg ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc
    29461 gtgatcaata atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc
    29521 atcaataaaa actggtggat cacactccct ttattttgag tagtatactt gacaacgatc
    29581 cggcgatcca ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct
    29641 gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag
    29701 aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa
    29761 cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa
    29821 tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat
    29881 aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc
    29941 aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag
    30001 aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa
    30061 agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt
    30121 cttttgatgt ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc
    30181 atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac
    30241 tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc
    30301 atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc
    30361 catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg
    30421 cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg
    30481 taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca
    30541 taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat
    30601 aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa
    30661 ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa
    30721 ctaatctgct cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt
    30781 cttgtaaaaa aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga
    30841 ttactcaagc agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca
    30901 ggtgataaag atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa
    30961 ccagagtcct tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg
    31021 agagcttttt tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt
    31081 tcttctgtaa tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga
    31141 ttcactcgca aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga
    31201 aaaactgaag aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa
    31261 taaatttttc catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt
    31321 aataaaaagt aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa
    31381 gttttcagca tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac ttccggccat
    31441 acttcttgta tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc
    31501 atacacccac gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc
    31561 atgatcatgc tatacacacc caccgaccac ggtttcaaga aaatcacaat ctgcataaat
    31621 tccctaaaag ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag
    31681 ggaagcagca aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat
    31741 tcatcaaatt tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag
    31801 taacggacaa gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa
    31861 agcccaaatc cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa
    31921 ataggaatga tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca
    31981 aattgctgga taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca
    32041 taaagaatca acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt
    32101 tgtaaaactg atcctttttt agacatctat attccccaca tcttttttca tttattaaaa
    32161 ctgcctcct
//
LOCUS       DAZUMO010000028        32048 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000028 DAZUMO010000000
VERSION     DAZUMO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.028"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG8ER_001337"
     CDS             6..245
                     /locus_tag="ACG8ER_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049931.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG8ER_001338"
     CDS             312..521
                     /locus_tag="ACG8ER_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049932.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG8ER_001339"
     CDS             581..1252
                     /locus_tag="ACG8ER_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5049933.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG8ER_001340"
     CDS             1305..3281
                     /locus_tag="ACG8ER_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5049934.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG8ER_001341"
     CDS             3294..4049
                     /locus_tag="ACG8ER_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5049935.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG8ER_001342"
     CDS             4046..4870
                     /locus_tag="ACG8ER_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5049936.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG8ER_001343"
     CDS             4880..5128
                     /locus_tag="ACG8ER_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049937.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG8ER_001344"
     CDS             5171..7270
                     /locus_tag="ACG8ER_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS5049938.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG8ER_001345"
     CDS             complement(7308..9365)
                     /locus_tag="ACG8ER_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFS5049939.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG8ER_001346"
     CDS             complement(9479..9691)
                     /locus_tag="ACG8ER_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049940.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG8ER_001347"
     CDS             9878..10345
                     /locus_tag="ACG8ER_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049941.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG8ER_001348"
     CDS             10342..10584
                     /locus_tag="ACG8ER_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS5049942.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG8ER_001349"
     CDS             10602..10904
                     /locus_tag="ACG8ER_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049943.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG8ER_001350"
     CDS             10976..13030
                     /locus_tag="ACG8ER_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049944.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG8ER_001351"
     CDS             13030..15642
                     /locus_tag="ACG8ER_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS5049945.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG8ER_001352"
     CDS             15639..18170
                     /locus_tag="ACG8ER_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS5049946.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACG8ER_001353"
     CDS             18222..18554
                     /locus_tag="ACG8ER_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049947.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG8ER_001354"
     CDS             18555..19148
                     /locus_tag="ACG8ER_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049948.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG8ER_001355"
     CDS             19311..21005
                     /locus_tag="ACG8ER_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS5049949.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8ER_001356"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8ER_001356"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5049950.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG8ER_001357"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG8ER_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG8ER_001358"
     CDS             24078..25283
                     /locus_tag="ACG8ER_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5049951.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMS
                     IINTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG8ER_001359"
     CDS             25299..25943
                     /locus_tag="ACG8ER_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049952.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG8ER_001360"
     CDS             25954..26760
                     /locus_tag="ACG8ER_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049953.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27011
                     /locus_tag="ACG8ER_001361"
                     /pseudo
     CDS             26782..27011
                     /locus_tag="ACG8ER_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27557..27832
                     /locus_tag="ACG8ER_001362"
     CDS             27557..27832
                     /locus_tag="ACG8ER_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049954.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27874..28296
                     /locus_tag="ACG8ER_001363"
     CDS             27874..28296
                     /locus_tag="ACG8ER_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049955.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28315..29907
                     /gene="mobP2"
                     /locus_tag="ACG8ER_001364"
     CDS             28315..29907
                     /gene="mobP2"
                     /locus_tag="ACG8ER_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS5049956.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29919..30254
                     /locus_tag="ACG8ER_001365"
     CDS             29919..30254
                     /locus_tag="ACG8ER_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049957.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30401..30595
                     /locus_tag="ACG8ER_001366"
     CDS             30401..30595
                     /locus_tag="ACG8ER_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049958.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30627..>32048
                     /locus_tag="ACG8ER_001367"
     CDS             30627..>32048
                     /locus_tag="ACG8ER_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS5049959.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKK"
BASE COUNT        11834 a         4966 c         6548 g         8700 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    13141 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    13201 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13261 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13321 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13381 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 cttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattactga agacagtgcc tattataaaa
    24721 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
    24781 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
    24841 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
    24901 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat ttattacgtg cacgaagata aggttaaaga tcaagtgaaa
    26461 gacgaagatt atgaacaaca cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcaa
    26881 gaagtgaatt atctaacaca acaatatctg agttgaagaa tggtagaaag agtttagata
    26941 ctttaaaatt cagatcattt gaagctctat atgtagctgc taaaaaatat tttacagctg
    27001 ataacaaata aaaaacagga gtattttatg tgaaaaaagt atataaaaaa tgcttatgaa
    27061 tcagctatat caccttaacc atttgttaat gagatgggag aaaacattaa ccacgattct
    27121 aacgcaaaca tcaagcacat aaaacattta acactcctcc tttctggctg aagatttcaa
    27181 ccaaacagga agacagcgta tcaattatag catacacttt ttcttaaatt catttttcat
    27241 atgacaaaat atttccttct aatcgacaaa gttagaagga ttttttattg tctaaataca
    27301 caaataacct taatttttaa agagcataag gaaacgtgat atcactttct cacgaaaggt
    27361 gttaggtagg ctgtgcctac cacgtttcta agaaagataa atgatatcaa aattttggat
    27421 ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg cagtaaaata agcgtttgtg
    27481 tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta cacatatccc tacacataat
    27541 aaatttggag gaaaatatgg acttattaat tagaaatatt gaagaaaagt atattaataa
    27601 aattgacaaa cgttgtgaag aacttacggt caagactggc aaaaaatgga gtcgaaatca
    27661 atacctaaaa gttttgatcg aaaatgattt tgatcacgct cttctgaatt ataaaaaaga
    27721 ccaatttgat cggttgttag aaaaatttgt ggatattcaa acttataata cgaaaacttt
    27781 agaagaatat attgcaacaa ataatcaatt aattggtttg ttaattgaat aataaaaata
    27841 gtattgtgaa tttataaaag gaggaaatca attttggcaa aagcaacaag aaaacatgta
    27901 tatctttacg atatgcatat cgaagtactt gatgaaataa tttctactca tgaagatata
    27961 tctaattata gtgaagcagt cagacatttg tgtatgatgt atgatccaaa aatcgaagaa
    28021 aaaataaatc caattcaaat taaattgaat gcaatgggaa aagaaatttc tattttaacc
    28081 gaaatggttt cagagtttgc ggatcttcat ttgaaagata caggaatact aattggtaca
    28141 aattcaactg tttatcaaga agcagaagaa cgtgtggaag gaaaaattaa atccaatcaa
    28201 accaaaaaat attcttctaa gttaaagaaa ccagtgaatg aatctgttca taccagagta
    28261 gatccatttg atccaacgaa gattctaaaa agataaaagt agaaggagaa cgtgatgaat
    28321 aaatcgcctg cagttattct tacttgtcaa tttaccttgc ccaataaaaa atctttttca
    28381 acatacattg attatatgac aagagaaaaa gcactggaag aaatggaacg acgtacgccc
    28441 gaagaagaac aggaactgca acgaatcaag aatgctttag aggattttta tattcctgaa
    28501 ggagaaactt tttcggaaag taagacggat aaagaattca gtgaaacgtc gaatgaagct
    28561 caatcgttga tggaacatgg aatcaatttt gacaagttag aggaacaaga ttttaccaaa
    28621 tatctttctt atatgacccg tcactatgcg ttagaacaaa agaaagaact gacgcaaagg
    28681 gaacaaaaag aataccaacg gttgaaccaa gcaatgaaaa aatatcaaac agagccacat
    28741 aaaaaagctt caggaaatct tcctggagtt ttttctatgg cttcagacga agtgaaagga
    28801 agtgatctga aggagatcaa acaaatcttc caaagaggac aacaaaaagg ctcaatcatt
    28861 tatcaagatg ttatctctca cgacaattcg tatcttgaaa aaatgggctt gtacgatccc
    28921 aaaacagata ccttagatga agaaggtttg aaagcagctg gaagaaaaat gatggagaca
    28981 ttattcgaga aagaacaatt gaatgaaaca ggttactgga tggcaacgat tcaccgtaac
    29041 actaagcata tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccgtat
    29101 actgagattg aaaatggtgt gagttatatc gtaccgaagg ggaagagaag acaagcaaca
    29161 ttagacgcaa tgaaaaccaa ttatgtacat gagttagaac gtttctctta tgaaaaggat
    29221 cgaatgatcg gtatggagaa acgaaattta ttgacacgaa aaagtgattt gcgtaacacc
    29281 ttaacaaaag tagtaaaaga accagcacgt tacgatcgac acgccatgaa cttattaaat
    29341 gaagtatata aaaccctacc tgaaaaacgt tcggaatgga attatggaga tgaaaatcga
    29401 accaagcttt caccatttac aaggggaaaa cttgatgagt taaccaaata tgttttaaca
    29461 aaagaacctg gatatcaaga atatatacag atttcaaaag cattgaaaga agaagccaaa
    29521 agcctttatg gggaaagcaa aagagaaaca aagaatgcag aaaaaaacgc cctctttgat
    29581 ttaaaaaagc gcacaggaaa tgcgatttta acagaactga agagacagga tagtcaaata
    29641 aaaccattaa tagaaagtgt caatcagatg caatctattt acaatgaacg atcctttcga
    29701 acactcaaac agttgccaac aaatgaatac aagtatagtt atcaagaatt cgtaaagcgt
    29761 aggaaacaat tccaacaaag agttgtcacg cagcgaagct tgcgtcaatt aaatcgaaca
    29821 attaatgcac atgaagaaaa ataccacgca agtaaagcat atgaagagca acagcgaaga
    29881 atccaaatgg aacaagaaag acaataggag gaagagaaat gaaacaatat ttagatcaat
    29941 ggaaagtcat agaaggatca ctaaaagaag aacgaatcga acaacttcct gattgtttag
    30001 aaaaagagca tttgtttcag ataagcgaaa tgttgagaaa cgaacagttt gatccaaatc
    30061 aatttttagt tgttgagtac ccagctactg gtgtgtattg ttgtaatcat gtgaagggtg
    30121 aaaaatattt tattattcaa gaatacgaag gtaagttagc gccttattat actacttggg
    30181 aaataaatga agagggaatc aataatttcc catgtgaaag cattgaagag tctatttctc
    30241 tcacggaatg ttaaataaat agagacgtaa aagagtcctg aacgtgattg tccaggactc
    30301 tttttcaatg aattgttttc cgccaattca ctgagtggtg cgattcaatt ttattatatc
    30361 aaaagaaagt gagattccaa agtgaaggga ggaagaagaa atgactcaga caacgattgg
    30421 tgttgtggtt gaaaaaagca gggataatct cgtttttgtg acagaaattc aaacaggtag
    30481 agcttttgta gtcacagaca aagctgcaaa agcgtatcaa aatggcaata ttttaacgtt
    30541 aaatctgact acgaagatgt tcgttgatgc agtagaaaat tatccatttg tttagacaaa
    30601 cgatatttta caaggaggaa acggacatgg cagaagaaaa agaaccacta gataatagtc
    30661 ggttgggaaa gtccaaaaga aaattagtac ggttgcaaaa cgaattaaat aatcaatttc
    30721 aaaagctatt tgatcaccaa aaattgacaa atgggcaacc aatgaatgac aaacgaaatg
    30781 gtcgttcatg gtttaaccag caagaaaaaa tagaaaataa aatacaaagt ttgagagaag
    30841 agatcaagca acaagaaaaa caggtagaaa aacttgaaag acaagaagag ctaaaggaaa
    30901 tgggctataa caagtacggc ggactggata tgacgatcga taatattcca agaatcaaag
    30961 aagaaatcga acgctttgaa aaaggtgaat cgactttttc agccgccacg atccgaaaat
    31021 atcaacggaa gttggaaaca ttagaacagc tgaaagaaaa atcagaaaaa ggaaaagaaa
    31081 atatcctccc tgaagtacaa gcgatcattg acagcggacg agtgacgcaa tggaaaaaaa
    31141 atccaaccat ttattttttg aaaggctatc gaaaagttgc tttagaactg aatgaaaacg
    31201 ggggatttga ggaatcaaaa aaataccaag ccacgtcgga agaggaaaaa gcagctgttc
    31261 gtgaattact tgaagaggct gcaccaactg attctctgaa ggagaaagaa aaagatcatt
    31321 tctatcgagt agaattcaac gaaaatgatc cagaaaaagg attgaaaagt tatggtgggg
    31381 agatcgtaac attagaattg atcgatcaac ttaaaacgtt ggataaccaa tacgctgcag
    31441 aagcaggtta ttataaattc tattttgaag aagtgaagaa cggagaagta gtcgatcaga
    31501 tccgaatgga tctaggggat ggcgtcaaca gtaatcaccc catctattta gagttagcaa
    31561 aattgtgcga acaagtacct gagaaggaac aaaaacaatc ggtcaaaaat gaacctgttt
    31621 cgaaagaaca aatgcagtcg aaagaaacaa aaacaaaaca aaagggctat caaaaacgaa
    31681 cggcagaaga gatcaaacaa gaagttaaat ccctttcgca acaggcactg gatgcggtga
    31741 aaaagcatac ccattcttct gaagacgtca aagaactttt gaactttatg tctcgttttc
    31801 cagaacgatc ctttagaaat caattattga ttgaggaaca atttccaggt gcaacggctt
    31861 gtttaggtcg tgccgcgttg aaaaaagaag gaatctatat caaaaaaggg gaacaaggga
    31921 acaagatctt tgtcagaaaa acggtcaaag gattttatga gaaaggacgt ggctttgttc
    31981 gagaaacaga agctacacca gaagataaaa aacgaatcga aagtggtcaa atcgaagtga
    32041 tcaaaaaa
//
LOCUS       DAZUMO010000029        30415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000029 DAZUMO010000000
VERSION     DAZUMO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.029"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..793
                     /locus_tag="ACG8ER_001368"
                     /pseudo
     CDS             1..793
                     /locus_tag="ACG8ER_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
     gene            933..1859
                     /gene="cysK"
                     /locus_tag="ACG8ER_001369"
     CDS             933..1859
                     /gene="cysK"
                     /locus_tag="ACG8ER_001369"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS5049960.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2095..2541
                     /locus_tag="ACG8ER_001370"
     CDS             2095..2541
                     /locus_tag="ACG8ER_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5049961.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2813..4165
                     /locus_tag="ACG8ER_001371"
     CDS             2813..4165
                     /locus_tag="ACG8ER_001371"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS5049962.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            4336..4623
                     /locus_tag="ACG8ER_001372"
     CDS             4336..4623
                     /locus_tag="ACG8ER_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5049963.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4849..5292
                     /locus_tag="ACG8ER_001373"
     CDS             4849..5292
                     /locus_tag="ACG8ER_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5049964.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5298..6482)
                     /locus_tag="ACG8ER_001374"
     CDS             complement(5298..6482)
                     /locus_tag="ACG8ER_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS5049965.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6750..7448
                     /locus_tag="ACG8ER_001375"
     CDS             6750..7448
                     /locus_tag="ACG8ER_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5049966.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7455..8132
                     /gene="nth"
                     /locus_tag="ACG8ER_001376"
     CDS             7455..8132
                     /gene="nth"
                     /locus_tag="ACG8ER_001376"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS5049967.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8258..8791)
                     /locus_tag="ACG8ER_001377"
     CDS             complement(8258..8791)
                     /locus_tag="ACG8ER_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049968.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9088..11469)
                     /locus_tag="ACG8ER_001378"
     CDS             complement(9088..11469)
                     /locus_tag="ACG8ER_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5049969.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11459..12106)
                     /gene="recU"
                     /locus_tag="ACG8ER_001379"
     CDS             complement(11459..12106)
                     /gene="recU"
                     /locus_tag="ACG8ER_001379"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS5049970.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12170..12709
                     /locus_tag="ACG8ER_001380"
     CDS             12170..12709
                     /locus_tag="ACG8ER_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS5049971.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12799..13218
                     /gene="gpsB"
                     /locus_tag="ACG8ER_001381"
     CDS             12799..13218
                     /gene="gpsB"
                     /locus_tag="ACG8ER_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS5049972.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13240..13607
                     /gene="rnpB"
                     /locus_tag="ACG8ER_001382"
     ncRNA           13240..13607
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8ER_001382"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13816..14982
                     /locus_tag="ACG8ER_001383"
     CDS             13816..14982
                     /locus_tag="ACG8ER_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS5049973.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15028..16524
                     /locus_tag="ACG8ER_001384"
     CDS             15028..16524
                     /locus_tag="ACG8ER_001384"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS5049974.1"
                     /translation="MKEKEFLQEVKEINLLQKALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16605..17288
                     /locus_tag="ACG8ER_001385"
     CDS             16605..17288
                     /locus_tag="ACG8ER_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5049975.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17933..19123
                     /locus_tag="ACG8ER_001386"
     CDS             17933..19123
                     /locus_tag="ACG8ER_001386"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS5049976.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19225..20178
                     /locus_tag="ACG8ER_001387"
     CDS             19225..20178
                     /locus_tag="ACG8ER_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS5049977.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20247..21596
                     /locus_tag="ACG8ER_001388"
     CDS             20247..21596
                     /locus_tag="ACG8ER_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5049978.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21779..22102)
                     /locus_tag="ACG8ER_001389"
     CDS             complement(21779..22102)
                     /locus_tag="ACG8ER_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5049979.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22231..23394
                     /locus_tag="ACG8ER_001390"
     CDS             22231..23394
                     /locus_tag="ACG8ER_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5049980.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23464..24813
                     /locus_tag="ACG8ER_001391"
     CDS             23464..24813
                     /locus_tag="ACG8ER_001391"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5049981.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24858..25289)
                     /locus_tag="ACG8ER_001392"
     CDS             complement(24858..25289)
                     /locus_tag="ACG8ER_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5049982.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25677..28319
                     /gene="alaS"
                     /locus_tag="ACG8ER_001393"
     CDS             25677..28319
                     /gene="alaS"
                     /locus_tag="ACG8ER_001393"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS5049983.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28519..29115
                     /locus_tag="ACG8ER_001394"
     CDS             28519..29115
                     /locus_tag="ACG8ER_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5049984.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29237..30385
                     /locus_tag="ACG8ER_001395"
     CDS             29237..30385
                     /locus_tag="ACG8ER_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5049985.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
BASE COUNT        10162 a         5250 c         6344 g         8659 t
ORIGIN      
        1 gaacgagtct tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag
       61 atgaacaagg atctcaaaca gttgctgatc ctcaagcgtt tatcgatcaa atcggctggt
      121 ctgcgtcaga agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt
      181 tgttggaaag tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg
      241 gtgtgaaagg aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaacgg
      301 acaatgggta acgatgaatg aaccattccg aaaatattct tcttattggg aatccttcca
      361 agaccatgcg aatgttcttc gttcaaccag tttttccacg ggcgattatc attatgctgg
      421 cgtgtggaaa agcaatacaa caagttttta tgatgcaaca gcgtacttga caggtcgtta
      481 tgctaccgat ccaaattatg gggccaaatt gaactcattg attaatactt ataatttaac
      541 ccgttttgat acgccatcaa caggttcggc taccacaagt acaacaacaa cgactactac
      601 gacgacttca acgtcatcat cttcaacaac agatgtaaat acaaataatg gtcagacata
      661 tacagtcgtg tcaggtgata cgctatggga tattgcacaa aaatttggca tgtctgttga
      721 tgatctaatg gcgaaaaatg gcatgtctat gtccagttat tctttattag ttggtcaaga
      781 actaaacgtt tgatttagat aaaaaggcaa agaaactacc atatatggta tatctttgtc
      841 ttttttttgc tagtcaaaga cgaatcactc gccataaatg aacagattgt attatgataa
      901 gtaagaagag caaagacaag gaggaattga agatgacagc tatctataac tcagtcaccg
      961 aattgattgg caaaacgcca atcgtcaaat taaataaaat cgtaccagaa gattcagcag
     1021 atgtttttgt aaagcttgag ttttttaatc caggaggaag tgtaaaagat cgtattgctt
     1081 taagtatgat agaaaaagca gaacacgatg gtctactgaa accaggtgat actatcattg
     1141 aacctacttc tggaaataca ggaatcggat tgtcgatggt aggagttgca aaaggatata
     1201 aagtaatcat cgtgatgcct gagacgatga gtatcgaaag acgtttattg atgaaagggt
     1261 acggcgcaga gttgattttg actcctggtg cggatggtat ttctggatca atcagagaag
     1321 cagaacgttt ggcaaaagaa aacggctact ttttaccatt acaatttgaa aatgaagcaa
     1381 atcctcttgt gcatgagaag acgactggtc ctgagataca tcaagcattt ggagtaaatg
     1441 gcttggacgc gtttgttgca ggtatcggta caggaggaac gatcactggc gctggccgag
     1501 aattaaaacg tgtgtatccg aaaatcgaat taatcggggt agaaccagca gaatctgcta
     1561 ttttagaagg gaaagaagcg ggacctcaca aaatccaagg aatcggtaca ggttttgttc
     1621 ctaaaacact ggatacttct gtgtatgata aagtcctttc tattagtgga gacgaagcga
     1681 tggaaacagc tagagaagtt gggcgaaaag aagggatttt agtaggcatc tcatctggcg
     1741 ctgcaattgc tgccgcgcta aaagtcgcaa aagaattagg aaaaggcaag aaggtattag
     1801 ctgtggttcc cgacaatggt gaacgttatt tatctacagc cctttaccaa gagatataag
     1861 gcacttggtt tttctccaga taggtttatg taaaaaactt ttaaagatga aaagaacaca
     1921 caaaaattta tgatgagaaa aattatttga atagagacgt aaaatagtga acgcgactgt
     1981 tttacgtctt ttttcattaa aatacaataa aattgaattt tcttttcatg ttaagtggat
     2041 tttaaaatgt aaaaatagta tgataagacg agaataacaa cgtggggtgt ttacatggaa
     2101 aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag agaacattcg aatcacacct
     2161 caacggtacg cggttttaga atatttgatt gaacatcatt cccatccaac ggctgatgag
     2221 atttataaag cacttgaaca tcggtttcca aatatgagtg ttgcgactgt ttacaataac
     2281 cttcgcttat ttacttcgat tggatttgtt caagaaatga agtacggcga tgcttctagc
     2341 cgatttgatt tcagttcaaa gcgtcattat catgtaattt gtgaaaactg cggtaagatt
     2401 gttgattttc attacccagg gcttgaagat atagaaatgg ctgcgagcag actgactgat
     2461 tttgagatta atgaacatcg gttggaattg tatggactct gtccggattg taaagagtca
     2521 gcaaaaaagg cgcaaatgta aaaacagttt ctataagtgt tatacctaaa aaaagctagc
     2581 tgtgtaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatatca
     2641 aagtgaatat tatcacaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2701 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2761 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     2821 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     2881 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     2941 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3001 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3061 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3121 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3181 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3241 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3301 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3361 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3421 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3481 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3541 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3601 caaagtgaat gaatacatgc agacaagtga tccagacatt tttgcagctg gagactctgc
     3661 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3721 tcgtcaaggt ttacttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3781 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     3841 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     3901 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     3961 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4021 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4081 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagaagcgct
     4141 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4201 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4261 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4321 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4381 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4441 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4501 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4561 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4621 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4681 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4741 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4801 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     4861 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     4921 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     4981 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5041 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5101 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5161 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5221 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5281 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5341 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5401 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5461 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5521 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5581 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5641 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5701 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5761 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     5821 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     5881 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     5941 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6001 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6061 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6121 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6181 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6241 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6301 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6361 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6421 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6481 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6541 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6601 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6661 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6721 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6781 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     6841 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     6901 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     6961 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7021 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7081 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7141 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7201 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7261 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7321 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7381 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7441 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7501 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7561 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7621 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7681 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7741 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7801 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     7861 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     7921 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     7981 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8041 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8101 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8161 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8221 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8281 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8341 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8401 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8461 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8521 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8581 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8641 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8701 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8761 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     8821 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     8881 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     8941 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9001 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9061 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9121 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9181 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9241 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9301 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9361 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9421 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9481 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9541 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9601 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9661 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9721 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9781 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
     9841 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
     9901 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
     9961 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10021 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10081 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10141 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10201 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10261 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10321 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10381 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10441 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10501 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10561 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10621 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10681 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10741 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10801 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    10861 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    10921 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    10981 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11041 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11101 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11161 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11221 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11281 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11341 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11401 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11461 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11521 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11581 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11641 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11701 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11761 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    11821 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    11881 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    11941 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12001 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12061 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12121 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12181 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12241 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12301 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12361 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12421 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12481 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12541 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12601 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12661 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12721 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12781 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    12841 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    12901 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    12961 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13021 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13081 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13141 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13201 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13261 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13321 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13381 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13441 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13501 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13561 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13621 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13681 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13741 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13801 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    13861 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    13921 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    13981 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14041 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14101 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14161 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14221 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14281 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14341 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14401 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14461 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14521 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14581 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14641 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14701 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14761 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    14821 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    14881 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    14941 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15001 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15061 gaaatcaatc ttctacaaaa agctttagga atcctcgatt gggatacaca aacaggtatg
    15121 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15181 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15241 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15301 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15361 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15421 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15481 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15541 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15601 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15661 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15721 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15781 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    15841 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    15901 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    15961 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16021 ttttatcgtt tattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16081 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16141 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16201 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16261 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16321 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16381 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16441 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16501 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16561 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16621 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16681 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16741 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16801 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    16861 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    16921 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    16981 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17041 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17101 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17161 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17221 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17281 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17341 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17401 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17461 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17521 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17581 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17641 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17701 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17761 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    17821 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    17881 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    17941 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18001 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18061 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18121 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18181 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18241 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18301 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18361 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18421 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18481 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18541 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18601 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18661 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18721 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18781 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    18841 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    18901 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    18961 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19021 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19081 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19141 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19201 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19261 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19321 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19381 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19441 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19501 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19561 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19621 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19681 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19741 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19801 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    19861 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    19921 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    19981 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20041 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20101 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20161 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20221 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20281 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20341 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20401 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20461 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20521 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20581 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20641 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20701 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20761 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    20821 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    20881 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    20941 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21001 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21061 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21121 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21181 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21241 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21301 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21361 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21421 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21481 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21541 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21601 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21661 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21721 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21781 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    21841 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    21901 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    21961 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22021 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22081 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22141 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22201 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22261 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22321 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22381 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22441 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22501 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22561 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22621 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22681 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22741 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22801 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    22861 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    22921 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    22981 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23041 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23101 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23161 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23221 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23281 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23341 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23401 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23461 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23521 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23581 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23641 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23701 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23761 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    23821 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    23881 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    23941 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24001 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24061 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24121 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24181 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24241 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24301 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24361 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24421 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24481 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24541 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24601 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24661 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24721 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24781 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    24841 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    24901 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    24961 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25021 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25081 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25141 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25201 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25261 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25321 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25381 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaatt aagtacgaaa
    25441 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25501 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25561 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25621 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25681 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25741 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25801 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    25861 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    25921 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    25981 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26041 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26101 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26161 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26221 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26281 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26341 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26401 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26461 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26521 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26581 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26641 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26701 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26761 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    26821 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    26881 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    26941 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27001 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27061 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27121 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27181 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27241 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27301 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27361 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27421 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27481 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27541 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27601 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27661 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27721 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27781 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    27841 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    27901 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    27961 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28021 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28081 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28141 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28201 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28261 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28321 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28381 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28441 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28501 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28561 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28621 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28681 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28741 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28801 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    28861 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    28921 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    28981 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29041 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29101 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29161 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29221 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29281 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29341 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29401 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29461 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29521 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29581 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29641 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29701 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29761 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    29821 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    29881 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    29941 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30001 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30061 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30121 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30181 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30241 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30301 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30361 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagt
//
LOCUS       DAZUMO010000030        30193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000030 DAZUMO010000000
VERSION     DAZUMO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.030"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(414..1358)
                     /locus_tag="ACG8ER_001396"
     CDS             complement(414..1358)
                     /locus_tag="ACG8ER_001396"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5049986.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1654..2598)
                     /locus_tag="ACG8ER_001397"
     CDS             complement(1654..2598)
                     /locus_tag="ACG8ER_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5049987.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2819..4033
                     /locus_tag="ACG8ER_001398"
     CDS             2819..4033
                     /locus_tag="ACG8ER_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5049988.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4055..6070
                     /locus_tag="ACG8ER_001399"
     CDS             4055..6070
                     /locus_tag="ACG8ER_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS5049989.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6153..7526)
                     /locus_tag="ACG8ER_001400"
     CDS             complement(6153..7526)
                     /locus_tag="ACG8ER_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5049990.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7552..7836)
                     /locus_tag="ACG8ER_001401"
     CDS             complement(7552..7836)
                     /locus_tag="ACG8ER_001401"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5049991.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7838..9853)
                     /locus_tag="ACG8ER_001402"
     CDS             complement(7838..9853)
                     /locus_tag="ACG8ER_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5049992.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10032..10337)
                     /locus_tag="ACG8ER_001403"
     CDS             complement(10032..10337)
                     /locus_tag="ACG8ER_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS5049993.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10426..11457)
                     /locus_tag="ACG8ER_001404"
     CDS             complement(10426..11457)
                     /locus_tag="ACG8ER_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5049994.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11664..12539
                     /locus_tag="ACG8ER_001405"
     CDS             11664..12539
                     /locus_tag="ACG8ER_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5049995.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12691..13533)
                     /locus_tag="ACG8ER_001406"
     CDS             complement(12691..13533)
                     /locus_tag="ACG8ER_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5049996.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13625..15502)
                     /locus_tag="ACG8ER_001407"
     CDS             complement(13625..15502)
                     /locus_tag="ACG8ER_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5049997.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15499..16920)
                     /locus_tag="ACG8ER_001408"
     CDS             complement(15499..16920)
                     /locus_tag="ACG8ER_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5049998.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16923..20312)
                     /locus_tag="ACG8ER_001409"
     CDS             complement(16923..20312)
                     /locus_tag="ACG8ER_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5049999.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20569..21960
                     /locus_tag="ACG8ER_001410"
     CDS             20569..21960
                     /locus_tag="ACG8ER_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050000.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22033..22680)
                     /locus_tag="ACG8ER_001411"
     CDS             complement(22033..22680)
                     /locus_tag="ACG8ER_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5050001.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22688..23374)
                     /locus_tag="ACG8ER_001412"
     CDS             complement(22688..23374)
                     /locus_tag="ACG8ER_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050002.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23380..24453)
                     /locus_tag="ACG8ER_001413"
     CDS             complement(23380..24453)
                     /locus_tag="ACG8ER_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050003.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24675..25349
                     /locus_tag="ACG8ER_001414"
     CDS             24675..25349
                     /locus_tag="ACG8ER_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5050004.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25354..26694
                     /locus_tag="ACG8ER_001415"
     CDS             25354..26694
                     /locus_tag="ACG8ER_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5050005.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26843..27829)
                     /locus_tag="ACG8ER_001416"
     CDS             complement(26843..27829)
                     /locus_tag="ACG8ER_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS5050006.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28553..29584
                     /locus_tag="ACG8ER_001417"
     CDS             28553..29584
                     /locus_tag="ACG8ER_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5050007.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29693..30118
                     /locus_tag="ACG8ER_001418"
                     /pseudo
     CDS             29693..30118
                     /locus_tag="ACG8ER_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
BASE COUNT         8958 a         5650 c         5419 g        10166 t
ORIGIN      
        1 aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa tctgcttatg
       61 caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa ttgaatgata
      121 aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga atcattgtat
      181 ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta
      241 ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt cggaccgatt
      301 aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa ctgcttaaca
      361 gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat
      421 acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct
      481 tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca
      541 cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca
      601 gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag
      661 taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt atcagcaatc
      721 acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta
      781 ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata
      841 tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga
      901 gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc
      961 caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat
     1021 cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa
     1081 tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca
     1141 tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata
     1201 ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact
     1261 aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct
     1321 gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata
     1381 actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat
     1441 tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata
     1501 aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa
     1561 taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt
     1621 ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg
     1681 agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat
     1741 acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc
     1801 gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt
     1861 actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt
     1921 aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc
     1981 tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata
     2041 gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc
     2101 aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc
     2161 ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa
     2221 atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc
     2281 ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca ccacgtcact
     2341 ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc tatacatata
     2401 attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac ggtgtttact
     2461 gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag
     2521 atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat
     2581 tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga ctccctcctt
     2641 tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata tgcaaagcta
     2701 tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga ttccgctatt
     2761 gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg aaaggaacat
     2821 gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta ttttgacttc
     2881 tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca cgcgttcttt
     2941 atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt ttgttttgat
     3001 tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg gattagtgat
     3061 tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt tacagtattg tcttagcttc
     3121 aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca gcatgattgc
     3181 tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg catttcaagg
     3241 atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg gttggcaata
     3301 taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc
     3361 tacacctgaa acaactgaac ttcaatcgca tggcacagcc ccaacaagac aagtaatcaa
     3421 aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa
     3481 tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa
     3541 tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg
     3601 ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat
     3661 cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct
     3721 aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc
     3781 gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac
     3841 tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact
     3901 tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt
     3961 agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt
     4021 gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag
     4081 atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg
     4141 ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt
     4201 caagtttaag agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg
     4261 agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc
     4321 agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt
     4381 tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga
     4441 atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa atcaaatcta
     4501 ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag gaatcttatt
     4561 cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg accatatcga
     4621 atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg attattgcta
     4681 ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt
     4741 gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc
     4801 tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt
     4861 atcagaagaa gccttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga
     4921 tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt
     4981 tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca
     5041 gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta
     5101 tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat
     5161 gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg
     5221 gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt
     5281 attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg
     5341 agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa
     5401 cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg
     5461 gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa
     5521 agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca cacagattac
     5581 tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat
     5641 cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt
     5701 ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc
     5761 ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta
     5821 ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct
     5881 agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga
     5941 aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa
     6001 tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat
     6061 aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat
     6121 attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt
     6181 gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt
     6241 ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact
     6301 agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg
     6361 ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct
     6421 cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct
     6481 actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt
     6541 cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg
     6601 ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat
     6661 gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct
     6721 gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct
     6781 ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag
     6841 agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc
     6901 tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg
     6961 atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt
     7021 tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc
     7081 atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca
     7141 taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa
     7201 atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa
     7261 gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg
     7321 aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc gatcagtact
     7381 aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga
     7441 agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac
     7501 acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt tttattcttt
     7561 tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg
     7621 tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa
     7681 ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc
     7741 cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt
     7801 tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga
     7861 tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag
     7921 aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg atagcagcta
     7981 aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca ggttgttgaa
     8041 gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca tgaggtaatg
     8101 cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt
     8161 tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg
     8221 cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag
     8281 atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata
     8341 cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg
     8401 acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt
     8461 gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact
     8521 ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt
     8581 caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt
     8641 gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg
     8701 ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta
     8761 tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa
     8821 gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca
     8881 tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa
     8941 agacattcat caataattca cgaaacgaag caaattctac ggccatcagg aattccatgc
     9001 gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc
     9061 cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg
     9121 aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca
     9181 gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca
     9241 aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg
     9301 ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat
     9361 acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt
     9421 ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca
     9481 gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc
     9541 ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat
     9601 aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag
     9661 taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata cggatacggg
     9721 tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt
     9781 cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa
     9841 ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa
     9901 atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta ccgaacgatt
     9961 tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg
    10021 atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc aaatttttct
    10081 ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt
    10141 gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca
    10201 gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct
    10261 aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca
    10321 ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata
    10381 agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc
    10441 tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc
    10501 atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg
    10561 gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat
    10621 ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat
    10681 gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt
    10741 ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga
    10801 aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc
    10861 taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg
    10921 atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa attcatgcgt
    10981 attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc
    11041 taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt
    11101 ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact cttctgcttc
    11161 ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt cgagtgccat
    11221 ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg
    11281 atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac
    11341 tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa
    11401 aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag
    11461 tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata
    11521 atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt tggtgaaatt
    11581 aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta
    11641 agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt
    11701 ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa gctgcagtct
    11761 acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt
    11821 tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg gggcgggaag
    11881 ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct ttttcaagtg
    11941 aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag tcaataaaaa
    12001 aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta ttgaacaagg
    12061 cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg aaattccaga
    12121 ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt gattcttctt
    12181 ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac agcggtcgca
    12241 tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct accttgctga
    12301 ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt gttatatcgc
    12361 aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc ttcgattacg
    12421 tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat atccgcaact
    12481 tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg aaaacttaag
    12541 aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt tgatatcatc
    12601 ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac ggaacgattc
    12661 gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt tgaacgattg
    12721 acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca atccatacag
    12781 gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt ttgcatgata
    12841 aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag gtgtacaagt
    12901 taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat cagtcggttc
    12961 taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt cgatatagaa
    13021 agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg
    13081 atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg ttccttccaa
    13141 taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg gcaaatggac
    13201 attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg ttctatcctt
    13261 tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac cttcttttgc
    13321 taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt cttcattttc
    13381 tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat tattcagcgc
    13441 gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga aaagcatcag
    13501 cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga tgactgtttg
    13561 ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa
    13621 aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc agtagaagga
    13681 ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga agtgtacctt
    13741 tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca taagattgtt
    13801 catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc gctttcgttt
    13861 cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg ttacttgtgg
    13921 ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt gtggatgggt
    13981 cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat tctgctccag
    14041 caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg cccccagtaa
    14101 cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca tttgcttggt
    14161 tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa acgaatttca
    14221 atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc gtgaatccgt
    14281 ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca tcatataagg
    14341 cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca tgagtatcga
    14401 taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata cctaatggga
    14461 tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt ccattcaatt
    14521 gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct actggtgttt
    14581 ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat
    14641 caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta acgatcaact
    14701 ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa gaatgaccag
    14761 taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca tctgtgacac
    14821 tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata aattctgctc
    14881 catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt ccatcattac
    14941 cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg tatttataag
    15001 agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg tttgcggcag
    15061 ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc ccattttgtt
    15121 ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa gcaactggtg
    15181 taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct cctttatctc
    15241 gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt
    15301 ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga tcgggtaaat
    15361 cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca aacacttttt
    15421 ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct aacataacgt
    15481 ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa acaaccagat
    15541 tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg tttcgtttgt
    15601 ttttggaaga aatggacggc tattttcttt tttattgact actttcaata tactgtccgc
    15661 ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt cacttaaaat
    15721 ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat
    15781 tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca
    15841 cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac
    15901 tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc
    15961 tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg ttccgttgtt
    16021 gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag
    16081 tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg
    16141 tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact
    16201 attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc
    16261 aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa
    16321 atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc
    16381 atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag
    16441 aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg attcaatgaa
    16501 aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc
    16561 atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg aagacgcacc
    16621 gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc gtagatggta
    16681 aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg
    16741 taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa
    16801 aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctacctgagt
    16861 aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat
    16921 atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca
    16981 atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact
    17041 tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca
    17101 gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact
    17161 ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca
    17221 ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag
    17281 tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct
    17341 tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca
    17401 ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg
    17461 aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcga
    17521 tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg
    17581 tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta
    17641 agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca
    17701 acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca
    17761 tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat
    17821 ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg
    17881 acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa
    17941 tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga ggaatttttc
    18001 gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt
    18061 tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga
    18121 ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca
    18181 gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc tggtttaatg
    18241 attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca
    18301 aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg
    18361 atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc taattcattg
    18421 gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg
    18481 aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt
    18541 cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc
    18601 gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt
    18661 tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt
    18721 ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct
    18781 ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca
    18841 gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg
    18901 agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg
    18961 ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc
    19021 gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact
    19081 ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt atgtccattt
    19141 ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt
    19201 gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt
    19261 actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt
    19321 ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact
    19381 tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca
    19441 agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg
    19501 ggtgtacttg tttcttttgc atactttcgt atcgcatagt ctgcttgatt accggttcct
    19561 ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca
    19621 tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta
    19681 ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc
    19741 gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg
    19801 acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac
    19861 acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg
    19921 atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata
    19981 gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca
    20041 tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct
    20101 ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt
    20161 ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta
    20221 gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa
    20281 ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt
    20341 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
    20401 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
    20461 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
    20521 gagggagaaa aacatatgat caggttgttg gaaggaaact atagaaagat ggaaaaaatc
    20581 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
    20641 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
    20701 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
    20761 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
    20821 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
    20881 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
    20941 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
    21001 atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
    21061 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
    21121 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
    21181 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
    21241 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
    21301 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
    21361 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
    21421 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
    21481 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
    21541 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
    21601 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
    21661 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
    21721 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
    21781 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
    21841 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
    21901 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
    21961 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
    22021 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
    22081 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
    22141 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
    22201 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
    22261 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
    22321 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
    22381 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
    22441 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
    22501 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
    22561 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
    22621 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
    22681 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
    22741 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
    22801 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
    22861 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
    22921 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
    22981 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
    23041 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
    23101 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
    23161 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
    23221 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
    23281 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
    23341 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
    23401 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
    23461 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
    23521 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
    23581 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
    23641 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
    23701 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
    23761 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
    23821 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
    23881 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
    23941 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
    24001 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
    24061 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
    24121 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
    24181 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
    24241 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
    24301 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
    24361 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
    24421 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
    24481 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
    24541 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
    24601 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
    24661 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
    24721 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
    24781 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
    24841 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag actccagtat
    24901 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
    24961 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
    25021 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
    25081 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
    25141 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
    25201 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
    25261 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
    25321 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
    25381 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
    25441 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
    25501 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
    25561 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
    25621 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
    25681 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
    25741 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
    25801 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
    25861 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
    25921 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
    25981 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
    26041 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
    26101 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
    26161 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
    26221 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
    26281 caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc aacattagaa
    26341 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
    26401 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
    26461 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
    26521 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
    26581 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
    26641 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
    26701 tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg cgaataaacg
    26761 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
    26821 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
    26881 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
    26941 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
    27001 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg tctgttccct
    27061 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
    27121 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
    27181 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
    27241 ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
    27301 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
    27361 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
    27421 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
    27481 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
    27541 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
    27601 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
    27661 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
    27721 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
    27781 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
    27841 ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta taggagacga
    27901 ttgttttcta caaatattta gattggaatt tgattaaaac aatttaattg aaatttgaaa
    27961 acatttatgt taatgaaagt catattctaa taaaatgtca tttgtttcta tatgaatttt
    28021 tatgaaaaat ttcatataat ttccactcgt tgtgatggtt taatttatcc aatccatcaa
    28081 taagcaaaca acttatataa aaaaggatta attctcaata taattgctcc taccatagaa
    28141 taaatcttca tgtaaagtaa tgaagtacca actatttcta atacttcttt aagaattatc
    28201 ccaacaatat cgttagacaa accattattg aataaatgga gagccaatcg attctgaaaa
    28261 aatgttttct aatttttcgg aaataaaaga aagagcctcc tgccattaga acgaaccaaa
    28321 tccatttcat tgctcatgaa gtaaccacat agctaaaaag aggataaatc aattaaaaga
    28381 tcaaaagaat gactgcacta ataaaataac taaaaatgta tcttcttact tcatatcttt
    28441 tagctagtac ttagttaaaa atttgcttcc taagatcaag acaaactgtc tatcttcatt
    28501 tgatgaaatg cggtaaaata tagaaaatga acactattgg agggaatttg taatggacag
    28561 caaaactaga aaacaactgc aagaaaagtt aaagcaacgt acacacttgc tcccttccgt
    28621 ctttcaacag tttttagaag aaaatcgttc ttccctttca ttaggcagtc gctatgaata
    28681 cgcaaaagat tttcatctgt ttactgatta tttgagaaaa gtattttctg aagaagtgga
    28741 tgatcagctg attatccatt tagaaaaaca agattatcag aatttcttag ctaaaatcta
    28801 tcgacatact cgttcgttcc aaactacagc gaaccaagaa acggagcaat tgtttgaaaa
    28861 tagctattac gggatttcaa ggaagcaata ttcattaaat cattttttac gatttttgta
    28921 tcaaaaagga ctgattgaag aagagatttc tcttttgggt acaagtttac cggaaacaac
    28981 caaagcagct ccgcgatata tcgatgcaga acttttcaaa gattttgagc atttgtttat
    29041 cccgattgaa ggctttcaaa caagcagaga agctagcttt gaagaaaaga atagaccgag
    29101 gaaccttgca attactgcca ttcttctgta ttgcggatta aagatccatg aagtagtaga
    29161 acttaaacgt aaagatgtat ctttgaccaa gcagattctt aatctgaata gaaaagaaaa
    29221 cagactgaat aaagtaccaa taccaaaaga agcgatgcca tttctggaga actattttaa
    29281 gttaacagca caaaatacgg acgaaaattc ttttgttttt cgttcctcac atgatcagca
    29341 aatttctcct cggactattc gccaaatttt gagcaaaatt acagtatata aaaacgtatc
    29401 gccagaacta tgcagaaaaa catatcgtta ttatgcagaa ctatatttag atgatgctga
    29461 cgcggtaaat tatttatgtg gtaatcgata cctctcatcc cattcttttc aagatatctg
    29521 ggaaaagatg gttgattttt cttatcacga attagcggca ttcgttcggg tttcttccat
    29581 ataaaacaaa tgtatgatct ttttttcaca ataaactatg ttattatata aaggattatg
    29641 ttacgattat cttgaatttg tatattactt aacaaaagat aatggagtgt gaatgatgga
    29701 gaccataacg tattcccctt tgaacttatt tacgaatttt caagaagctg cagctaactt
    29761 ttccgagacc ccgattatct ttgatgagcc actttcagct ttcccagaat taggacttga
    29821 aacaacttac aaaaaatgct gcgaaagtgt gttgaaacgg gcttatcaat tggctcatct
    29881 tggagtgaaa tccggagaca aagttattat ttataagagt tccgcttttg acacatacct
    29941 tctagctgtt tctgtgtctt accttggtgc agtgcctgtc atgacttctt atcatctacc
    30001 aacaacaaca atggaagtat ttatcgatcg tttagaagac cctttcattt tatttgatga
    30061 tgagacaaaa aagcgagtga gagaaatttc aaatggaaca aaatcaaaac agattcctaa
    30121 cccgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg gtaaatcaac
    30181 aaaaaaacgc ata
//
LOCUS       DAZUMO010000031        29949 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000031 DAZUMO010000000
VERSION     DAZUMO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.031"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..328)
                     /locus_tag="ACG8ER_001419"
     CDS             complement(26..328)
                     /locus_tag="ACG8ER_001419"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5050008.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="ACG8ER_001420"
     CDS             complement(353..2446)
                     /locus_tag="ACG8ER_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5050009.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="ACG8ER_001421"
     CDS             complement(2935..3699)
                     /locus_tag="ACG8ER_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS5050010.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG8ER_001422"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG8ER_001422"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS5050011.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="ACG8ER_001423"
     CDS             complement(4704..5780)
                     /locus_tag="ACG8ER_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5050012.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="ACG8ER_001424"
     CDS             complement(5804..6397)
                     /locus_tag="ACG8ER_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS5050013.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG8ER_001425"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG8ER_001425"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS5050014.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="ACG8ER_001426"
     CDS             complement(8067..8366)
                     /locus_tag="ACG8ER_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS5050015.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG8ER_001427"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG8ER_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS5050016.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="ACG8ER_001428"
     CDS             complement(10121..11032)
                     /locus_tag="ACG8ER_001428"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5050017.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="ACG8ER_001429"
     CDS             11152..12009
                     /locus_tag="ACG8ER_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5050018.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="ACG8ER_001430"
     CDS             complement(12196..12558)
                     /locus_tag="ACG8ER_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS5050019.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(12747..12929)
                     /locus_tag="ACG8ER_001431"
     CDS             complement(12747..12929)
                     /locus_tag="ACG8ER_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050020.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            12904..14343
                     /locus_tag="ACG8ER_001432"
     CDS             12904..14343
                     /locus_tag="ACG8ER_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050021.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="ACG8ER_001433"
     CDS             complement(14350..16902)
                     /locus_tag="ACG8ER_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS5050022.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="ACG8ER_001434"
     CDS             complement(17016..18347)
                     /locus_tag="ACG8ER_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5050023.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="ACG8ER_001435"
     CDS             complement(18313..19791)
                     /locus_tag="ACG8ER_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5050024.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG8ER_001436"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG8ER_001436"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5050025.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="ACG8ER_001437"
     CDS             22401..23828
                     /locus_tag="ACG8ER_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050026.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="ACG8ER_001438"
     CDS             complement(23920..24285)
                     /locus_tag="ACG8ER_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5050027.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="ACG8ER_001439"
     CDS             complement(24282..24647)
                     /locus_tag="ACG8ER_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5050028.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="ACG8ER_001440"
     CDS             complement(24839..25087)
                     /locus_tag="ACG8ER_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050029.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="ACG8ER_001441"
     CDS             25086..25214
                     /locus_tag="ACG8ER_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS5050030.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="ACG8ER_001442"
     CDS             25271..26965
                     /locus_tag="ACG8ER_001442"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS5050031.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27065..27238
                     /locus_tag="ACG8ER_001443"
     CDS             27065..27238
                     /locus_tag="ACG8ER_001443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050032.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="ACG8ER_001444"
     CDS             27616..28749
                     /locus_tag="ACG8ER_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS5050033.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="ACG8ER_001445"
     CDS             28780..29100
                     /locus_tag="ACG8ER_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS5050034.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="ACG8ER_001446"
     CDS             complement(29129..29593)
                     /locus_tag="ACG8ER_001446"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5050035.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29726..>29949)
                     /locus_tag="ACG8ER_001447"
     CDS             complement(29726..>29949)
                     /locus_tag="ACG8ER_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5050036.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         9033 a         6212 c         5170 g         9534 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaaac ggaaaacaat
    29701 cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga
    29761 tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg
    29821 ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta
    29881 cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa
    29941 cactaacta
//
LOCUS       DAZUMO010000032        27801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000032 DAZUMO010000000
VERSION     DAZUMO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.032"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(349..522)
                     /locus_tag="ACG8ER_001448"
     CDS             complement(349..522)
                     /locus_tag="ACG8ER_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050037.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHLFIFAYILAMIA
                     FLSGGWLIIYLLS"
     gene            complement(525..923)
                     /locus_tag="ACG8ER_001449"
     CDS             complement(525..923)
                     /locus_tag="ACG8ER_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050038.1"
                     /translation="MQLRRNKNYTTIILEDFTDQELLIVGKTKADFIKKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKELYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1139..2194
                     /locus_tag="ACG8ER_001450"
     CDS             1139..2194
                     /locus_tag="ACG8ER_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050039.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2214..2567
                     /locus_tag="ACG8ER_001451"
     CDS             2214..2567
                     /locus_tag="ACG8ER_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050040.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2749..3036)
                     /locus_tag="ACG8ER_001452"
     CDS             complement(2749..3036)
                     /locus_tag="ACG8ER_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050041.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3049..3867)
                     /locus_tag="ACG8ER_001453"
     CDS             complement(3049..3867)
                     /locus_tag="ACG8ER_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5050042.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4105..4326
                     /gene="relB"
                     /locus_tag="ACG8ER_001454"
     CDS             4105..4326
                     /gene="relB"
                     /locus_tag="ACG8ER_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5050043.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4328..4594
                     /locus_tag="ACG8ER_001455"
     CDS             4328..4594
                     /locus_tag="ACG8ER_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5050044.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4761..5246
                     /locus_tag="ACG8ER_001456"
     CDS             4761..5246
                     /locus_tag="ACG8ER_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050045.1"
                     /translation="MGWYGAYSSANEVIEEKKGYAKNGLEIVDTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            5737..6021
                     /locus_tag="ACG8ER_001457"
     CDS             5737..6021
                     /locus_tag="ACG8ER_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050046.1"
                     /translation="MLKATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6028..6273
                     /locus_tag="ACG8ER_001458"
     CDS             6028..6273
                     /locus_tag="ACG8ER_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050047.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6295..6549
                     /locus_tag="ACG8ER_001459"
     CDS             6295..6549
                     /locus_tag="ACG8ER_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050048.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            6631..6816
                     /locus_tag="ACG8ER_001460"
     CDS             6631..6816
                     /locus_tag="ACG8ER_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS5050049.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            6883..7278
                     /locus_tag="ACG8ER_001461"
     CDS             6883..7278
                     /locus_tag="ACG8ER_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS5050050.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7295..7645
                     /locus_tag="ACG8ER_001462"
     CDS             7295..7645
                     /locus_tag="ACG8ER_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050051.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            7667..8041
                     /locus_tag="ACG8ER_001463"
     CDS             7667..8041
                     /locus_tag="ACG8ER_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050052.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTFITLEENLVISDAGIERN
                     IKRVSIKITLNKPTDKKLSEAF"
     gene            complement(8349..8747)
                     /locus_tag="ACG8ER_001464"
     CDS             complement(8349..8747)
                     /locus_tag="ACG8ER_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS5050053.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(8939..9706)
                     /locus_tag="ACG8ER_001465"
     CDS             complement(8939..9706)
                     /locus_tag="ACG8ER_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5050054.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(9742..10902)
                     /locus_tag="ACG8ER_001466"
     CDS             complement(9742..10902)
                     /locus_tag="ACG8ER_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5050055.1"
                     /translation="MKITEKNVNPKFEPTESNQKMFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKTNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLEDHFTQYTFDQLV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIAELRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTDIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKFMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            11951..12754
                     /locus_tag="ACG8ER_001467"
     CDS             11951..12754
                     /locus_tag="ACG8ER_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5050056.1"
                     /translation="MSQEVTENVIDNDVDLVSDQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNVFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFESFDYEKIYAKKGRKVDRLRFTFSEPESNLPSISMHNWLEED"
     gene            13361..13816
                     /locus_tag="ACG8ER_001468"
     CDS             13361..13816
                     /locus_tag="ACG8ER_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050057.1"
                     /translation="MDAINWHEFVNYMEKLLDDLKSFSIADAVKHGLPVSTTMKMRGL
                     KVPDEIIKLRTKTIIETAKIVDSMNLDLKGKKTTYEKDFVRFLKNVIGKYLGKKSFTE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIVEYLENKEQKKGTKKGR"
     gene            13935..14993
                     /locus_tag="ACG8ER_001469"
     CDS             13935..14993
                     /locus_tag="ACG8ER_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS5050058.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15035..16984
                     /locus_tag="ACG8ER_001470"
     CDS             15035..16984
                     /locus_tag="ACG8ER_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5050059.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDIAKLEEELKSIKGQPVAVYTVTMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNTILFATTNAGAE
                     MYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHLV
                     FDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGDT
                     DAEIIAKPSIAESKENKFVFSVGGV"
     gene            16987..20676
                     /locus_tag="ACG8ER_001471"
     CDS             16987..20676
                     /locus_tag="ACG8ER_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFS5050060.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     AKLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGRGSVKDMVAGGLGAVGAQGIAD
                     RLNNMGDSNADAKTIADKVVPETPASVPFNGGGVGGTINNTSFKDLVRQDQLRNNSQQ
                     YSDHLGRMVDEKGNFVDENGEVLKDSKFGKDAKGNFIDAKGRLVDEDGNLIDTNGKPL
                     EDNEDGNIGMDSQGRLIDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRL
                     VDEDGNLVDEFGKKLGKHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPI
                     AVAIPDDESDEVILDQAHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNV
                     PVEGQSNELHNNFDNVADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVN
                     QANDSETNTGLGMAKDNQSIREPKKTTESSKSNKSKSTSNKTGKSKEPSKAKSAVEYL
                     SKTGKNVYTSADKALAKAPKGSLAGMTYEAKNEAIKLGKGTAKVAKVTAKGATKTGKA
                     VKKSTMSVKDYIAKYSEQKPTDKKS"
     gene            21087..21425
                     /locus_tag="ACG8ER_001472"
     CDS             21087..21425
                     /locus_tag="ACG8ER_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050061.1"
                     /translation="MFDSIKNLVSGMTPEMKTLVQAILYLMATFFALKPGFTAMKHFG
                     DAKWGEGLKYVGAVVAIFIIPVITTVMLLTMGTKTGNDLNQKANMISALIPVVGIYLV
                     TKFPKEEQLG"
     gene            21552..21773
                     /locus_tag="ACG8ER_001473"
     CDS             21552..21773
                     /locus_tag="ACG8ER_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050062.1"
                     /translation="MLVHANEVLTSGFKGTDLAKIMNMNVNQFYDYRNGNKKIEKARL
                     ETLIKFEKAYLYVLDKQKRTIDRKKGVLQ"
     gene            21773..23938
                     /locus_tag="ACG8ER_001474"
     CDS             21773..23938
                     /locus_tag="ACG8ER_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050063.1"
                     /translation="MAKFKDITESINYVAETVPVVVLSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYVVIEDVQKLSSEEFPDEEDAILDSEKYVVLLKSSLN
                     YVVDLPEKFKEKLLAGVRFMDSNGQLLVIPDDPNMFEREVEEATDFYSAVDQLEVATK
                     GLERIAETFNSSDAVEFEAVAEAETEAVPDEEEALFEDNDEATEAGNQEASIEETQTE
                     TYQQPKIDEKPESQSDKENYVYEAEKIVSESDVPKNLDADLLEKDFEQIIANKFQPVV
                     LDEVSDPVDLNEEMRSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLLLKGK
                     QMLADELLRIEEATSLSGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLEAELKKLEQRYDAEHLPEVKQQEDEFRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIEKSLTEVQEKTLEIVEKTKAENEAEFHDTKEKTTDVVVK
                     YFANKEEVEKLVAKDTNELESKLVAQQNIIEDYKNRVKEFKDLADGYKLNSQKLTEQS
                     EALREDLSRARNDLSQMAEKTKSLVVTTENQTESKRDFTSKKMATIIAVSAMACGTIL
                     GSSYFISHNHQEAQAQTETSISRVENSLGAKTASTSTTDNNYEATPTTEFKVGEDVVI
                     SIDGKTTKAEVLSIDGDKAVVKAPDGNQYKIQAHLTEKN"
     gene            23950..24180
                     /locus_tag="ACG8ER_001475"
     CDS             23950..24180
                     /locus_tag="ACG8ER_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5050064.1"
                     /translation="MREENIVYPVIENQSNTKSSQLLQEVQVVYPVNELPQTNETVSK
                     SISLIGLLIVATALTVGVYRKLKVKGSISDGR"
     gene            24170..25036
                     /locus_tag="ACG8ER_001476"
     CDS             24170..25036
                     /locus_tag="ACG8ER_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050065.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVDWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFP
                     EEIIAQEGKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIV
                     DQQERFVSEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSEIYALK"
     gene            25054..27012
                     /locus_tag="ACG8ER_001477"
     CDS             25054..27012
                     /locus_tag="ACG8ER_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050066.1"
                     /translation="MELMSRSKLLNFRKKKHKKEQNADSSKAVNNTYVSDLTSLVGYQ
                     FTPSYIRTGNRWGTIVKLVNTYGMNRNYEFGWMVKAIPEVKVEGVKAYLIEADKPVSQ
                     KDQQEIMHENVGGSTNSYENNLNDKNSDAEINKTVMRIIDLNTASLLDSTANKIIDWK
                     LEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSFGGDQRDLFDRLLSEPVGSVYEY
                     TSMSSDFAGNDHAIRKGLDDADGWAIGTMSTSYASGVAFMAMDRSFKGKVLVASHKDA
                     TIKGYSEEISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGETFVAKPSLNSV
                     IEKINLAEEGLNSLELFGDRKDIGDLYRRNLDKLTQIFYLLADRELNPATRVLLKKEL
                     GNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEIDRII
                     VLKDTLRTALDSFSKMFNQRTTLKEIGKSDKLQFYYNLSDLRTEINVMEAQFLNTFDF
                     ITSQAKKGDIVMIHGFDRLSVETVGLIKKRLTMLEEDGVRLAFLFDTIGSGESKDGIE
                     TADIFNTDGKIYQSFESDFNYTIIGKMTSEELMKYEKKVAPARKGEDDQKLSVDLRQY
                     LTSGRSNKFQIRRPADLTNNFVIGEFII"
     gene            27100..27708
                     /locus_tag="ACG8ER_001478"
     CDS             27100..27708
                     /locus_tag="ACG8ER_001478"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5050067.1"
                     /translation="MKSNIKIIAGDKMKEKENLVWYFNRNPHMLITGRTATGKTYLLK
                     RMADYLSQVGIVSASFDKTEFEEILSSSRKEMEDRFEYIKENRFKMFDVSYSDLDMKP
                     YFVLIDELDSRLLLLEKENPLAYNYLLLIIKDIVLKGRVVGVFFIAVRQGKQPLLESI
                     EDNMLTRIFLGDSKGNGTICFDGNTKKFQTELVTEDFFSVDC"
BASE COUNT         9876 a         4353 c         5167 g         8405 t
ORIGIN      
        1 atattggttt cgccttcaga ttaatactgg atcatattaa tctgaataca atatatgaac
       61 taataaatag cttaacctcg ctattttgtt ttttctgatc attatctaca cagcattaac
      121 aaatagaatt ttaaaaataa aaaaagtaag acaaaaaaag tatcaacata aacatataca
      181 tatacacgtt tatgttgata cacttaattt gtcctttaaa aagcactgat ttttaaattg
      241 cgttatttta taacccgaat acagtctgct tgttaggcat tgtccttaaa ttacattttt
      301 cataggttat ttaatcattt aatctgttga ttattcttga tctaaaactt agcttaacaa
      361 atatataatc agccaaccgc ctgataaaaa tgcgatcatt gctaatatat aagcaaagat
      421 aaaaagatgt tcgatcagct taaaccctga tgcgattaag cagaccaagt aaaatagaaa
      481 ttctcttact gataatggta attttctaac tagcatactc ataattaaca ccccaaagta
      541 atattgtatt ttgaacattt tacatccagt acagcatcaa tcaaccattg ccaatcaaaa
      601 atacaaggct tcattgaagt atgtttttca gatgaaaaga tataatataa ttcctttgga
      661 ataatatttt cagttgttgg gatcaagcta aatctaattc catctaactc atagctacca
      721 gctttctctc cattaaaaat ttccttagca ctttcttcta aagaatcaaa atatttttcg
      781 acagcaacct ttgaagcttt tactattgaa gcatttccct ttttgataaa atctgctttt
      841 gttttaccga caataagtaa ttcttgatca gtaaaatctt ctaaaataat ggtagtgtaa
      901 ttcttatttc ttctcaattg catcaggatc actccttaat ataatttatg tttttataat
      961 atcataataa gataaattta caagtatttg tttacacctt tttatttaca ctttattata
     1021 attaattata cttttatgat tgcatttttt tcttataaac agttgcaact aatagtaaat
     1081 aggtgtaaac tattttcgta ccgagatacc ataaacacaa acagaaagag gtcataccat
     1141 gaaagaacca ttattgtttt ccgttgactt agggaataaa tcagtaaaag aaatgagtac
     1201 ccacgtttct ccactctcac ttccctctcg tattttgaac aaaaatatca ttaattcagg
     1261 acgtttttct aatataggtt cctcattaaa acgatcacta aacgtttctg aatatagcct
     1321 agtaaacact actgaaagct attactttgg gaaggatata cacgagctag gaaaagatga
     1381 ctacgtgata gaatcactag gttttgggat tagcagatac aagcaggaaa cctaccaaaa
     1441 tatcctatct ttctctttag ctaacctagc acgtttagaa gatcaatctg atgacgtttt
     1501 aaccgttaat ctagttttag gtctgccttc atccgactat accgaaacaa tcgtcaaata
     1561 cgtgatgcaa caagccacac gacaacattc agttgaagtt gacggtaagg cttataatat
     1621 aaacgtagat accgtttggg tactgccaca gccaatcgga acgctatata acctcctact
     1681 tgctgatgat ggtagcgtac aagatgaaac cttgatcagt cagaagatcg gttttgttga
     1741 tattggtggt ggcacgaaac tttttgatgt tgtccaaaat tttgaaattg acgaaaacaa
     1801 tcgtgaacaa aaggataccg gcatttttac tttatataat cgtatcgtaa atatgcttga
     1861 ccttccgatc agtcctaatg ctttccaact tgaaaagatt atccgagaag gcaaagaaaa
     1921 aggcgtatat ctatttaagc caagcggaaa tgaaagtttt gatatttcag aagtagttga
     1981 aagagaaata aaaacataca caaatatggt tgttgcagaa gttttagcta cattcaaaaa
     2041 cttcacgacc ttagataaag ttgtctttac tggtggtgga gcttctatta tcgacaaaca
     2101 acttgtaaaa gataaaattc cgcatacctt gtttgttgaa gacagtgaat tttctaatgt
     2161 aaaaggcttt tacaagtttg gtttaactca ataaaacagc gaaggagatc aacatgaata
     2221 agggaaaatc agagcgttat gtagttataa tcaatagaga aaaggttagc gagttacctt
     2281 tagcagagtt tcttgaatcc gaatatgaaa cactaggcgt taaatactct cagacgatca
     2341 aaaaagcttt gaacctatat cggcgtgtaa agaacaatga agttacaatt attgaagcag
     2401 gcaacgatag aaacgatagc aaagtagcag aagtaaagaa taaaccaact gatcataacc
     2461 ctattcaaca agagcatcct tcaacggtta catccgatca ttcagcaaag aaagaaaagc
     2521 caatgctaaa agggttcaat gcaccaagta acaaagactt actttaaaat gaaaaaggcg
     2581 aggttacaat acctagcctt tttattatgt acaaatataa acataaacat atacgtatac
     2641 atgtatataa acatgtatac gtatatatcc ataaacacgt atatgaaatt tagttaagct
     2701 atacctaacc gcttattcca ataaaaaaag tgatcagctt taaactgatc actcaccaaa
     2761 aacttcatca tacaagttat ctatcatttc accaagtaaa gctgaatctg atcgaattcc
     2821 taacttttcg ctgattttag atagcttctt tctttgcgat ggttgaagcg tgaattgata
     2881 atttttcttc ttttcagaac tatcactttt tagtttagct accaaagaac ttgttgtttc
     2941 tttaggtgtt tgtttaggtt cttcaactgc ttcaaccact tcaggaactt cttctacttc
     3001 agcttcagcg aattttttaa atctattttc tttcacttca atcactcctt attcttgtgc
     3061 cgtatcaatt atcttgttgt atagcgtaaa cgtgctttca aagaaatcct tattcttacg
     3121 atagatattc ttatccttag cgaactcagc aatattctta ccttctaagc tagttgcatt
     3181 aagcaattcc ttctcttgta catatccaag ataattagat aacgttggta ccaattctag
     3241 gaactcacga cttgaattag tattatgttt gatcctgcta ccgatcgtag cgtatttagt
     3301 ttccatcagt gtttcaccag tcttaatatc aatcaactgt tttgctagtg catcaatatc
     3361 tttaccaagc ttcagcaaag cttcaaaacc atctttagat ggatcagaaa tgcctacgac
     3421 aagatcagat acagcccaag cattttgagc tagtagacct gcatcattat gagtatcaat
     3481 cagaattaga tcatacttct ctttaagctg ttcatagtta acaacgatcc atctaaacag
     3541 tagggttctg ttgttaggca tatcacgcat ttgcatctca acttgtttta aatcttcacc
     3601 tgctactaaa atatccatgt tctcatttat agctaaaggt tcagctgatc catgaagata
     3661 taaatgattg attgtattct ctcttttcgc tttactgaaa gcatcaggaa agcgttttga
     3721 taggttttgc gacttatctt catcaatata cagcaccttc atacctaaat tttcgacagc
     3781 atatccacct agattgtaga ttgttgttgt tttacctgtt ccaccttttt caatgtttcc
     3841 agtaatgatt gacaacttct tttccatgat taaacctccc ttattaacct tttgtaactc
     3901 cattatacac tataaacacc tttatgtata tgtttatata cataaaggtg tttatattta
     3961 tatttgcttt tatattctat aacataaaca tatatatgta tatgtttatg tatatgttta
     4021 tgtttatgta tatgttcata tatataacta ttgcatctgt catacaatcg tagtacaatg
     4081 taattgaaaa ggagttgata aactatgcca gtaataagta ttcgtgtatc taaagaagaa
     4141 aaagattttc ttgaaaaagt tgctgaattc aacggagata acatttctga atttatgcgt
     4201 agtaattccc tgcaaacagc agaaacgtta gtagactttg ctacctacaa ggaactaatg
     4261 aaagagcatg aggaaaacga tcaaagtatt tcccacgaag atatgctaaa agagttaggc
     4321 ttataagatg agttaccaag taagatatga acgtgaagct caaaaaacgc taaaaaaaat
     4381 ggataagttt caggcaaagg ttatcctaaa ctggatcgag aaaaatcttg taggcactga
     4441 tgatccaaga aagcatggta aaggtctaac agctaataaa tcaggctact ggcgatatag
     4501 ggtaggtgca taccgtataa tagctgatat tagcgagcat gaagtgacaa ttcttatatt
     4561 gtctattggt cacagaagtg aagtttataa gtaacagaca agctgataag gcttgtctgt
     4621 ttttttgtta acccaacaaa atcactgttt acgatcccac tttacctaaa aaagaacaat
     4681 atcctcttgt tattatacac accagtgtgt ataatataag tgtaaacaag ctaattaact
     4741 ttcaaaagga gcgatttttt atgggatggt atggtgctta ttcatcagca aatgaagtaa
     4801 tcgaagaaaa gaaagggtac gccaaaaatg gtttggaaat cgtagatacc aaagtaacga
     4861 agacctatgg aattatcctt ttcaaagatg cagaaggaac ctacgatatt gacagcttca
     4921 tctttcgaga tgaaatgtac aaaccaatct acttcttaaa tgacagtcct gcttacttta
     4981 agaaaattcc aaaaaaatgg ttgaaattct ttgtcggtga tcaagaaaaa atggttaagc
     5041 gacaactaga tagaattaaa gaagaaagca ataaaccaaa acttgatgac ttattagttg
     5101 caggtaaaac ttactcaatc tgggatggat cgtatgtcgc tgtttatcag tataaactca
     5161 aacgtagctt tattttccga ctacaagatg gatcactcac tcggttcact cgactatcag
     5221 caggagatgt taaggaagta gcctaaaact taaatacata aacataaacg tgtatacgtt
     5281 tatgtttatg tatatgtata tgaatataaa cgtattaggt acgaacgctt ttaaatgctt
     5341 ttctgggctt tgattttagg gtagggctaa aacatcacca tctgatccaa aacagctcag
     5401 aaacgcattt aaacgtatct gataccaata ttacagaagg agtgtatcgt atgattaacc
     5461 tatttctttt agtgacagct agtacattat tctttactgg aatatcactt ataatttcct
     5521 atttgatcta taagaaagcc gaaacttaca caagcaaagc aaccggacta gccgtttcat
     5581 cctttttaac tttgctgact ttttttgtta ctctatcgtt ttcttttgtt tagcacagct
     5641 gatccatcag caaaaaacct ctctttacat tatacacact agtgtgtata atgtaattgt
     5701 aaacaagcta attaactttt aggaaggaat gacaatatgc taaaagctac attacagcaa
     5761 cctactcacg ttgctattgc agaaagctat acaattaatg caggggtcgt taaacagctg
     5821 atcaaggatc gagaattcaa aaagtttaac agtcgtcaag cgtataacag acagttaaag
     5881 aaagctattc taaagcacaa aaattccttc atcgggaaat tggaaagtgt ggatgttaaa
     5941 actgtttcaa cggcattttc agttgttgaa gtgactattg acggtcgctt gatttctcta
     6001 tttgcttttg gaattaattg atagggagtg tggactttgg aaggtaagac ttttgcagaa
     6061 atttggactg aatttattga ggtaccaact cgcattgatc cgataacttc tgaaaaggta
     6121 ttggatgcag acttctacaa tctcaaagca ggtactagcc ttgaatgcgt ttggatgtat
     6181 ttagaagagc atttcaaagg cgtaacgctc ggtcacgtct tataccacaa tggtgccgag
     6241 tgggatgcaa ttggaaatat ccatttttct taatacaaga aaggagaaca caatatgaat
     6301 tataccgatg aagaacaaat cactttggaa tacgttttca caaatgaaga aggggaggaa
     6361 attgaaaaag tcttgatcat ttcatcaaaa gacttggaga aggctttggc ttatgaacca
     6421 tacgaatcaa ccgtaactgt cattgaattc ttagatacct atacttctaa tgatacttgg
     6481 gaaattatga atatttgcaa cgctaataac attcaatttg ctgaaaaaac caataaatca
     6541 ttttattagt tttgcaataa aacatcctct ttttctattg attccataca caacaatgtg
     6601 tataatagat tttgtaaagg gagggtagct atgcctaaga cagcaagaga aattgtaaaa
     6661 attctcaaat ctaacggctt caaaaagact ggacaaactg gcagtcatgc aaaattttat
     6721 aattccaaga ctaaaagaac ggtcgttgtt ccgatccaca atggtgatgt accactagga
     6781 acagaagcta atatctggaa gcaagcaggg ctttaacagc cttgccttcc tatttggtat
     6841 taggcatact atcctctctt acactagaaa aggagcgagg aaatgaagac aaaaaatatt
     6901 ttttatccgg caatcttcga gaaagaagga gaagcttata acatcgtttt ccctgactta
     6961 ccggaaatat ctacctttgg aaatagtctt gaagaagcat atttaaacgc taaggatgct
     7021 ttaggattag cgctatatag tgtaccagat gcagaatttc ctaaaccatc agcagtggaa
     7081 gaattaactt tgaaagccaa ccaagtggta gtaatcatcc aactaaacat taaacttttc
     7141 cgaagaagct taaatacaaa aacaattaga aaaaatgtta gtgttccgga atggttagtt
     7201 cttttaggga aggagaaaaa cataaacttc tctcaactac ttcaaaaagc actagaagaa
     7261 gagttgcttg aaaagtaaaa aggagaccga acctatgaaa gctacacact taaactattt
     7321 ctctcgaacg ttaatcaata atctattaaa agatacaact ggcattagcg ttagtaccaa
     7381 acactttgcc aatgatctaa ttatttttga ggataataac ttgcgaattg agatgtttat
     7441 tgaacaggca acccctaact caatacctga attctgctat acgcacactg aaaaaatgaa
     7501 acatgcaaaa tcagttaaag atgttgaagc attaagtgaa tcactaacga acgctttaaa
     7561 cctcgttaag aaggcaaaat tgttatttga atttcaaacc gactaccaca atgagttgct
     7621 tgctcaatct gaaattttag attaattgaa acaggagaag agaaagatga aacaaacaca
     7681 aacagaaacc gttaacgata cgcttgctga tcaattagca ggttattata aattatttga
     7741 gatgagtaaa gcacagcgct tgatccacta tctttggaaa tacaaaattt tgtttctttt
     7801 ttgggaagca acactaacat tctcagttct tttttataag tcagattttt ataatcaaca
     7861 cttagtttta gggagttttt tgcttatcta caccattttg atgcttaatt tatatacctt
     7921 catcacactt gaagaaaact tagtaattag cgatgcaggg atcgagagaa acatcaaaag
     7981 ggtatctatc aagatcacgt taaacaagcc taccgataaa aagctctcag aagcgttctg
     8041 agagcttttg acatttgctt gtataaaatt tatcctaaca cattacaaag cacctaattc
     8101 gagcttatat caagcaaatt tgctatttat tactcaatac atatctaaaa attacttgat
     8161 ggaaaactag atacatggta agcatgaaaa gaaaatcaat ttgagcagat gcagtataat
     8221 acatagcaat ttaaaagctc tcagaagcgt tctgagagct tttaatatat tctttgataa
     8281 tttgtctttc ttaaatccat acgtcctaaa acaagctgtt attgcttaat atgatcattt
     8341 gagagacatc aaatcaactt ttctctcaac aaatcattca aagctttcga cagattaata
     8401 ttctcttcag ccgctaaaac atttaaatag tgttccacag tcactgattt tcgatcgtaa
     8461 ttttgcttgt ttcgagcttt agcaactttc gtattagctt ttacgacaaa taaattaaca
     8521 ttctcagatc gcttgattct tgcatttaac tcttgaacag gcgtaggcga tggaatttct
     8581 ttttcttgca actctaaaag caccaagtat ccttccaata cttcttgcgc catctcaaaa
     8641 gcatcttcta aagtttcacc atgcgtatat aagtctggaa aatctgggaa tttaactgca
     8701 tattcatttc ctttccattc gaatactgca caaaaaaagt atttcatcgc attgcacctc
     8761 tctaattaaa aaataatttt tgctttttag tttcaaaaaa aagaatcgcc taccaaaaaa
     8821 attgataggc gatcacaagc taattaacct ctaaaggaga ttaatacctt gctcccaagg
     8881 cactacatat cttagcatac gtctgaagca tcttcaaacg agtaagatat attttgcatt
     8941 aacctaattt tttttctcta taaggttttt catcaaactc actagaaata ttaaactgga
     9001 cagcaaaatt gtaatttctg ccttttctaa ctttagcaaa atcaatcttc agcattgatt
     9061 tttcattaat ttcctcaact gattccctta aaacacgtct atcaaaattc gatattgttt
     9121 tatactcatt ttctgttttc gtaatctcac gaattttaac tatttcaatc aggggattct
     9181 tatatatttc agctttctca aaatctcctt tatttttggc gataagatat tttttataat
     9241 acatcgccaa atatttgtat aacacgatgc tatatttttt ttgaaaagat gctaagtctt
     9301 caaaaacaaa gaacggatac ttctttttga ttgagacaat taagggaatt acatcttctg
     9361 aaaaactgaa agatacacta tgctcttcta aatcccaatg aatccgatca atagcagata
     9421 cagttgtata cgtcttatat ttcttgtcta gattcgatat ttcataaact gcttgtcttt
     9481 gcaaagtgtt aactatggtt tttagttcag tttcaagatt ttgatcaaca tccatgtaat
     9541 taataatcgc tttaacaggt aaatctacta cgcctgtttt ctttagcttt gtgggcgaga
     9601 tataactgaa cagcaattga taaagttcat acgactttaa atccattttc atgtcaattg
     9661 ttgctaagaa ctcctcgttg tactttgtca gttcatcttt tttcaaaagc ttttccttcc
     9721 ttcacgcaag tttttcagtt gttaatcata aaggttcaca taaggtaaat aactcgtaat
     9781 tgatttttcg agatatttaa cagtattttt tattgtgtaa tcctccttat gagtagcaac
     9841 gtaagataga tgattgacaa ctgattcaaa tcctctatct ttggataaac gatcataaag
     9901 cggataaact ttattctgaa gccgactcat aaatttcaga tcgcataagt cggcagaact
     9961 gatcaaaaag tttttgatta gtaatttagt gtatttgcta ttcatagctt tcaagtacga
    10021 ttgattcatt ttctctgttt tatcttctgc gccttttaaa taagcagggt ctctttgttc
    10081 ttgcttataa ggtaccggta ctgacatggt acgtgtaagc aagaacttta tatctgtata
    10141 agtatgaccc ttcttgataa gctcatagtc aattttaaaa tcagtgtatt tattcagttg
    10201 ggcaatcggt tctttaatta cacgtctttc aaagtctcga aaagctttat attttttctc
    10261 aatactgaat ttctttcgta actctgcaat tgaaaaaacg gggtttttta atctatcaag
    10321 tgattttgta ctccatgtta agttatctct atttgcttca tacagattaa aatctttagt
    10381 gaataattga aaaaaactga tcgcatattt gcttgtcaag ttaactaatt gatcaaatgt
    10441 atattgtgta aaatgatcct ctaagttcgt taacaaaggc ataattgaac tactaaattt
    10501 aatagatact gcatcagaaa aatcattgaa ttcagtaatt tctagcggtg agatgacttt
    10561 gtattcagtc ttaccgttag ttttagttct ttgaatttta atgacagatt gctgatgcaa
    10621 tttttcaata acttgtttta atcttgaatg ttttgaagaa gatgaactag aagacatcaa
    10681 cgcaaataaa gcctttttag atactttgac ggtattatta aaaggcggat ttttagggtc
    10741 aagatgtcct atagcgtaaa gataaatttt ttgtggcata gcatccattt caacaatgct
    10801 atctacaagt tgattcgata aagtaacagt taaatcaaac attttttgat tgctttcggt
    10861 aggttcaaat ttcgggttta cattcttttc tgttattttc atacgattgc ctcttctttc
    10921 taagcttggt acgagcttaa aaacttggaa ctggtacgat tccattgaat aagtatagac
    10981 ctgttttcga tacgatacaa taagaaatgt atcgtaaatt tataacataa ctcaatctca
    11041 gtaaaacgca ttttattttt attaatcaaa tttcattgat aagacagcag aaatcgctat
    11101 tttagaacct ttacaaaata aaaatccttt tcaaaaaaaa tacgtaatta taaaaattca
    11161 caaaattaaa aaacaaatat ttttttagtt tttagaaata aattcacata ctttgtcttt
    11221 ttttgtatat attcggaata ccacatcgct ttttgtccat gtttgcatcg ctttttgtcc
    11281 acattcacat cgctttttgt ccatgttcgc atcgctttat gtccacccta aaatctattt
    11341 aagattgata taaagccttt ttggaggttg ctaaagtttt aaagattaaa atatatatta
    11401 taaatataaa taaaagggga agctgaagct tcctcctttt cctttttttc aattattcaa
    11461 aattacacaa gcaaaataaa ttgctagata gtgttagttt ttgttttaag gtttttaatt
    11521 tttgagattt tgaaataaga gaggaggaat cctcctttat taaatatctg ttaatatatc
    11581 tgttaaatat ctgttaatat atctgttagg ggggtcttaa acccttggga gagtaagtat
    11641 tgaaggggta tgaaggtaac acttttggac agtaaaggta acaaaagtgg acagtaaggg
    11701 taacattttt ggacagtaaa ggtaacaaaa gtggacattt gaggtatcaa atcaggataa
    11761 gacgttacct tcggttttct tgatttggaa atctaaaaag tagaaaacta gattagtaac
    11821 aaatctttgt tgactgttac ctttagttgt gatttaattt aggtaacaag cagaaaaaaa
    11881 gcgtttcctt taatttagta ccaaattaac atgattaaag ggatgcaaca agaagattgg
    11941 agagtaatca atgtctcaag aagttaccga aaacgttatt gataatgatg tagatttagt
    12001 atcagatcaa acaaatgcaa ttgttcgtta taacaatggt ttgaacacaa cagctttgcg
    12061 aggatttaaa cctgtagaaa tggatgtgtt ttgggctatt ttatcaaaaa tgaaacgtca
    12121 aggaacagca gaagtaactt ttgatttcaa cgtctttcgt tatttggtca aatacgacag
    12181 aagagaaaag gacagttttt attctgtgtt aaaagctttg agtgacaaac taggtacttt
    12241 aacctaccgc tttgaagatt cagatgtcta tgaacagctg tggctttttc aaaaattccg
    12301 aatagataaa cagcaagaaa cagttacaat caaggcaaat gaggagtttg aatttatctt
    12361 aaattcaatt ggcaaaaact ttactcgttt tgaacttgat aactttacaa aactaagaag
    12421 tagctacaca aaagagctgt atcgtcatct catgcaatat agaaataaag agtggcgaaa
    12481 tggctattgg gtagtcaaag ttgaagattt tagaagagcg ttaagtattc cttcaagtta
    12541 cagaatgaca aatattgaca aaagaatatt agagcaggcg aaagaagaat ttttagctcc
    12601 tgatgaaaat ggtatacagg tatttgaatc ttttgattat gaaaaaatct atgcgaagaa
    12661 aggaagaaaa gttgatcggc tcagattcac ttttagtgaa ccagaatcaa acttgccatc
    12721 catttcaatg cataattggt tagaagaaga ctgaaaagaa gcaggttgta attttaatac
    12781 aacctgcttc ttttgaaaaa agtatgggta acacttttgg acaataaatg caagaagttt
    12841 ttttgtgacc aaactttata ttagattact gtgattttgt gtgttagaaa agccctgata
    12901 gaaaagcaaa ttcaaacact ttttaatatg tatgggtaac acttttggac atgtcaataa
    12961 taaatatatt ttctttcgtc ttttgcctaa ctaaaggtaa cacttttgga cagtatagtt
    13021 ttgtaaacta attagatcgg gtttaatggg atatatctta gcatttatgt aagaatgaag
    13081 gtaacattaa tggacatgtc aactgtttga acaaaaaaat atagaatatt tggatctgat
    13141 ttttcatttt tccaaacgtt aaaaaatagt tagttatttt attaatagat agtcatcctt
    13201 aatgtttgaa atagcagaaa ataagatatt gtttgataaa tgttgttgtt ataaggaaaa
    13261 cttgaatttg ttaattgatt tatttatgtt attatgatac tataataaca taaatgtgtt
    13321 acgcccgaac ctcattttac aaaaatgaag gggtgagaca atggatgcta ttaattggca
    13381 tgaatttgtt aactatatgg agaaactact agatgatctg aaaagtttta gcattgctga
    13441 tgccgtaaaa catgggttgc cagttagtac aacgatgaaa atgcgagggt tgaaggttcc
    13501 ggatgaaatt atcaagctga ggactaaaac aatcattgaa actgcaaaaa ttgttgacag
    13561 tatgaacctt gatttgaaag gcaaaaaaac aacttatgag aaagactttg tacgtttttt
    13621 aaaaaatgtt atcggaaaat atctagggaa aaagtctttt actgaattga gagacgaaga
    13681 gattgtacca actccaatgg ttagcaaaat tcgcaaaaac aaagccgagg gaaattacta
    13741 tcttggcaca atagcaaaaa tcgttgagta tttggaaaat aaagagcaaa agaaaggaac
    13801 gaagaaagga agatagttcg tgtgataaat atgaataggg aagaatgtac cgaaatgata
    13861 aaaaaacccg aacctagaat taccgaaaaa ctgactgatt gcttgctctt ttacgaaagg
    13921 gggcaggcaa actaatggaa aaagatgtca attcagcatc cactaaagat caatacaata
    13981 tcattggagg attacttggt gataaggata agagaacacg tcatggatta cgcattatgc
    14041 ttgcgacatt tctaggggtg tttcttttat taacaggctt gggtagctac atttatcaat
    14101 taggaaacta taattattat cttgtcaaca atacaacacc aatcggctca tcattgacgt
    14161 ttgctcgatc aggagcagag atcactatct cagatgtttg gacagataaa actagagatg
    14221 taacggtggt caaacttggt tactcaggaa acgcaagaaa attgctatct acacaaggta
    14281 aaaattataa cttgtatatg gcaacaaaag aaaaagatcg tcctaagaag cttgaaatga
    14341 gttatgggat gttaagtacc gatggtgatg ctttcttgtt cctaaaaggt aaattagatg
    14401 aaagagctta tcaagttttt attgctaacc aagtatcact aatttcaggt gatgatacag
    14461 ctgatgatga taccaaagtc aatgtgaatg aatcagattc agtcaccaag gcactttctc
    14521 aatattctat gaatgatgtt gataatgaag gagtaatttc ttttgggaaa aaagcaaaac
    14581 cagttaatac tcctgataat atcaatttca gaataaatcc atactcagat acaactaacg
    14641 tatatcaagg aagcttcctt accaaagatg gcgatattga ttactcaaaa gttgtagctg
    14701 tgacaagtaa gaataatgtt gtaaataagc tagaagacca gttaaatgct tcaaatacta
    14761 acatcaagaa aatcgaagcc acaaaagctg agtatcttga acgtttacaa caaaatcctg
    14821 acgataaaga ttcgcaagaa agtattcgtc aactagacag tcgaatggag accgaaatta
    14881 aaacaatcaa tgatacacaa aaattgattg atcagtacaa aaatgctgac tttaacaaag
    14941 attcttttgg agatatgaag acaacattta agtacctaac cctgactgac tagaaacaaa
    15001 ttattcttat tttagaggaa gtgaacacaa acagatggaa aatagtaccg agttctttaa
    15061 aaatattcaa accgaattca atcatgctct cacactaatg aaaccaccga aacataagtt
    15121 agtggggcgt gatgacgaac tccatgagtt gtcactgatc ttaaatagac cggaaacacc
    15181 acaagctatc ttgttaggtg aagcaggaac aggtaaaaca gcactggcaa gtgaatgggc
    15241 taaaagacag cgacaagagg ggaagaaagt acaagtatat aaattatcaa tcggacaatt
    15301 gcgagttgga ggagatgacg tcctccaaac acgtttggaa actataattc caagattgaa
    15361 ggaatacgaa cgaattttaa aatccgagga caaagataat gaatttgtcc tctttataga
    15421 tgaatttcat aaagttgtca gttcatttgg gagcggtaat aaaattgggg gagattcgct
    15481 gaaggaacct ttagcaaatg aatcacttca gttgataacc gctacaacta gaaaagaata
    15541 tgaaaactat atcaaacgag atgatgcttt aggtagacga ctacaaccgt tagaactcaa
    15601 cgaattaaaa agtgatgtcg ttgtcgatat actaagacaa tggttgaata catcatctgt
    15661 caaagatgat cctgaagctt ccttgaaatt atccaaaaag attcctgatg aattgcttga
    15721 agaaatcgta caagcaaaca agctttatcg tgaggattta tccgaaccgg ctaaatcaat
    15781 tgccgtaatg gaagatttga ttgcgaagga tcgtgtagat ggcaatgcgt ataatcgaga
    15841 aagcctgtta caaatatttg gaaaatataa tattcaacta gattctgatt ttgatatagc
    15901 aaaattggaa gaggagctaa agtccattaa agggcaacca gtagcggtat atacggtcac
    15961 gatgttgttt aaacgaatga aacttggatt tgatcgtttt aaagggcgaa agcaaccaaa
    16021 agcaacggct ttgtttacag gatcaacagg ggtcggtaaa actgcgatgg ctaaagctgt
    16081 agcaagaggg ctatacggtg atgaaaataa agtcctttta gtaaatatgc cagactataa
    16141 aactaaagat gatgctatgc ggtttagaaa gcttatcgga acacatgtcc ggcatgatcc
    16201 atcagcaatc attgtttgtg atgaatatga aaaagcaggc gtagaaacac ttgatgcctt
    16261 tttatctatt ctagatgaag gaagaatcag ttttactgaa agaggatggg atgatcgaga
    16321 agttaatgat accgtctcac tttcaaatac gattttattt gcgacaacga acgcaggggc
    16381 tgagatgtac gattcgatga accgatacag ctcactatct cgtgttgcta atcaggaaaa
    16441 ttcaaatgag tacagcactg agcttaaaag agagtatcga aaggtagcat tagagattaa
    16501 acaagctcta attggtgaag gtctaaaacc cgaagtcatc aaccgatttg gacggattgt
    16561 tccattttgg gctttatcgg aacaaactct tttggaaatt gcacacaatg aaatattgaa
    16621 aagtgctgaa ttggtaagaa aacacttaaa cgtagactta cgccttccac cacagaaaaa
    16681 acatttctat ggtcaagaat tttatgcaga tgatctctcc atgcacttag tttttgatag
    16741 agcttcacaa gaagattcta aatcaggggg tgcaagaaat atcttgcatc tggtcgaatc
    16801 agaatttgaa gaagcaatta ctgaagcaat aattacctat cctaacgcca aaatatttga
    16861 cgttaaattg agcaaagcag gagggtttga agggaaaggc gatacggatg cagaaattat
    16921 cgctaaacct tccatcgctg aaagtaaaga aaacaagttt gtgttttcgg ttggaggggt
    16981 gtagctatga aatttaaaaa gtctttactt caaaatcgta aaattaagcg atgggttgta
    17041 agaggtttag ttatcgtggg ggcacttgca gttactttag gcgctccatt tggaatgaat
    17101 gatccaacgt ctgatgatca aggaaataaa caagaaacag ttgcaaatac gaatgtggct
    17161 tatgctgaaa gtaaaccggc agaaaaaggg gtatggacac tatatgctcg tatcctgatg
    17221 ggagataaag aggtcaatga tagtgccact aaaactggtc aaatgaattt acttggtcaa
    17281 ggaggtatta gtgctgactt tccttattct gatatttcca aacaaggtaa tgcaattgcc
    17341 ggtggtaacg caggtacgca attagcatca accttagcga cttataatta ctatgggtat
    17401 atcgaaacag tttcaggaaa tgcgattgca agtcaggcat cctcttttat tggtggtatc
    17461 ggtagaacgc ttggcagttt tatcggatca attagcttga tactttctaa tattgctgaa
    17521 tccatcaatg aactccttgg aaaatttttc atgagtatga atatattttc acttatgggt
    17581 tggggtaaag gtggatcgga tgtttctaat ccggtagcta aggcgttaac tagcttactt
    17641 aataatttgg gattaaacaa tcaattattt aaagcgatag caagcttatc tatgctcttt
    17701 tttacagcaa tactaggcta tcaaggaatg aaactgttgt cgaaagaggg tgttaactca
    17761 aagggattat caccagtact tgaaaaatgg attgttcgta tttttgtatt gtttgttttt
    17821 ctaccagttg caggatcgtt attgggagtt tatatcaatg gtattcagtc agcaaaagaa
    17881 gatggaaaat taccaacaaa tgttgttggt gattacatca ttaatacaag actttgggct
    17941 tctactcaaa atcttgctcc atcatccgat actggtggga atgtgccaaa cggaaaacca
    18001 acatctgaaa ttaaatttgt tgacactgac tatgaaccat ctaaaaaatc agaaatgatc
    18061 agaatgatca atactagcag ttacgctaga caaaataatg atctagattc caattcagtc
    18121 ggcatggcat tgttaaaaga atggtcaagt aatgatacgt ttaatgtcaa tacttatgcc
    18181 ggtgatgtga atgtagctga aaatgatgct tatatgggaa gagcgcctgc tgtttggaca
    18241 ggctataatt taggggatgg aaaagaagga aatgcaatta agaaacctac caatttaaat
    18301 ggttatattt ggtcagctaa tcaaaacgtt acagacgaca accgcaagcc tgatggaaaa
    18361 ggaacaacga ataactttga tgctactcaa ccttggggtg ttgctaacaa tgcaacgttt
    18421 agcacacagt cggtagcgtt gatgctccaa tcagcactgg atacatcagg ggctaagttt
    18481 tatgcgtata atatcgctcc atcaggaatt caaggtagtg ctaagaatat gtcaacagta
    18541 aagacagaat ggcgtacagc ttctttagtt ggtcatggag ctatgggtaa attcggctca
    18601 tggttagcga tgaacgctga atcaatcgta ttagcagtgc tttatctagc ttgtgcttgg
    18661 gcgatgttta acgttaatat tgtaaaagcc ttcaaacgtg agtttgtgcg agtttgggaa
    18721 gtagtcttaa cagggtctct tgccgcagga gcaggggtta tggcaatctc attaggagtc
    18781 gtaaccacat ctcttatcgg attacaatta cctatttttc tgatcacctt tgttcgtgca
    18841 tttggagata cggctgtagg attcatccct aacacattac ttggtgatgg tgtagcaaat
    18901 attgtacgat ccgctatggt cttaggcgta ggataccttc tagtctttaa gaaaggtgaa
    18961 aaggttaata ataatttgat taatatgctt attggattac cacttgaagt agcgtttggt
    19021 tttgacgaac gtgctaagtt attatttggc aatagaaatg atgctgttcg agttgtggat
    19081 ggcgtttcta aagcaatcaa aaaaagccaa ggggctaaaa atccttatta tgatggtggc
    19141 agaggatcag taaaagatat ggtcgcagga ggacttggag cagtcggagc gcaaggaatt
    19201 gctgatcgtt tgaacaatat gggagacagc aatgctgatg ctaagacaat cgctgataaa
    19261 gttgttcctg aaacgcctgc ttcagtacca tttaatggtg gcggagttgg tggaacaatc
    19321 aataatacct ctttcaaaga tttagttcgt caagatcaat tacgtaataa ctcgcaacaa
    19381 tatagcgatc atctaggacg gatggttgat gaaaaaggaa actttgtcga tgaaaatggt
    19441 gaagtattaa aagacagtaa atttggtaaa gatgccaaag gaaactttat tgatgccaaa
    19501 gggcgtttgg tcgatgaaga tggaaatttg atagatacaa acggtaaacc attggaagac
    19561 aatgaagatg gaaacatcgg tatggatagc caaggacgtt taattgatga caaagggcgc
    19621 ttggtcgatg acgaaggaaa ccttgttgat gaaaacggac gtaaattact taacggtgat
    19681 tttgggcagg atgtcaatgg tcgtctagtt gatgataaag gacgattagt tgatgaagac
    19741 ggaaatctgg tcgatgagtt tggtaagaaa cttggaaaac acagtaacgg ttataacgta
    19801 aatggcgata cagtcgatga agatggaaac atcattgatt caagagatgc tcttgtagca
    19861 gatcgaaata aagaactcga acaacaacag catacaccaa ttgcggttgc aatccctgat
    19921 gatgaaagcg atgaagttat tttagatcaa gcacatcaag ggttaagaga agaagcaaag
    19981 gataaagttg tttcagatca agcacaagaa gagctaaaaa ataaatcgaa tggtgaagat
    20041 gtttcaaacc aatatcatca aacggctgga aatgtacctg ttgaaggtca gtctaatgag
    20101 cttcacaaca actttgataa tgttgctgat cggaatgata cagtcttaaa agaaaaagaa
    20161 tcattaagac aagaaacaga taaagaccaa tcaaaattga agaaaacaac tattcctact
    20221 cctattggtt caacagttcc gaaggtcaat caagctaacg attctgaaac aaacactggg
    20281 ttaggaatgg cgaaagacaa tcaatccatt agagaaccta aaaagacgac tgaaagtagc
    20341 aagtcgaaca agtctaaatc tacttcaaat aagacaggga aatctaagga accatctaaa
    20401 gctaaatcag cagtagaata tctatctaaa actggtaaga acgtgtatac ttcagcagat
    20461 aaagctttag caaaagcgcc aaaaggaagc ttagcaggaa tgacctatga agctaagaac
    20521 gaagctatca aattaggtaa aggaactgct aaagttgcca aagtaactgc taaaggagca
    20581 actaaaactg gtaaagcagt gaagaaatct acgatgtcgg tgaaagatta tattgcaaaa
    20641 tatagcgaac aaaaaccaac cgataaaaag tcttaagaag aataaaaaga gggagcatta
    20701 atatgctcct tcttttttgt tcttctcttc aaggggaatg tcaagaaaaa cttaattgtc
    20761 ttcaaaaatt tcttttttat ttggtagtaa atctatgaaa tatttgcttt tttagttatc
    20821 ggttaataag agggcttatg ctagttagtt aaaatcgggt aactagtcat taaatgctga
    20881 taaataagca atttttacga aaaaaaaaga aaaaaatttg agggaaaata cctttcaatc
    20941 tacaaaaatt tatgttatta tgataacata aagatgtaaa tatcgaagcg tcttttctgt
    21001 attccgatca ttgacaagtg aacagagaaa agtttgcgat gatataatta tcttgtgaaa
    21061 aataatcgaa aggaaggatt tataatatgt ttgattctat taagaatttg gtttccggaa
    21121 tgacgccaga aatgaaaacg ttggttcaag ctattctata tttaatggct acattttttg
    21181 cattgaaacc aggattcact gctatgaaac atttcggtga tgcaaaatgg ggagaaggct
    21241 taaaatatgt tggagccgtt gttgctattt ttattatccc tgtaattaca actgttatgt
    21301 tgttaacgat gggtactaaa acaggtaatg acttaaacca aaaagctaat atgatctcag
    21361 ctcttattcc tgtggttggt atttatctag ttacaaagtt tccaaaagaa gaacaattag
    21421 gttaaaaggt aaattgagca aatttggagg atatgccgat ttgttcaatt tactttttgc
    21481 taaaaaacta cactcgaagc gtagtttttg aagtgaggga agacaataga tgtaagatgg
    21541 acagagaaaa gatgttagtt cacgcaaacg aagtattgac gagcggtttt aaaggtaccg
    21601 atttggcgaa gataatgaat atgaacgtaa accagttcta tgattatcga aacggcaata
    21661 aaaagattga aaaagccaga cttgaaacgc tcatcaaatt tgaaaaagcg tatctatacg
    21721 tgttagacaa acagaaaaga actattgacc gaaaaaaagg agttttacaa taatggcaaa
    21781 attcaaagat attaccgaat ctattaatta tgtagcagaa acagttcccg tagttgtact
    21841 ctctttgaag ccacaattac tcgaagtgta ccaaaaagaa gaaaaagaag cgaaggcaaa
    21901 aaaagctaaa gcaaaggtat ggaaagaatt cgatgcgttg ctgaccaaag atttaccaaa
    21961 gaagaattac gtggtgattg aggacgtaca aaagctcagc agtgaggaat ttccggatga
    22021 ggaagatgcg attctagatt ccgaaaagta cgttgtttta ttaaaatcaa gtctgaatta
    22081 tgttgttgat ttaccggaaa aattcaaaga aaagctatta gctggtgtcc gctttatgga
    22141 tagtaatggt caattattag ttattcctga tgatcctaat atgtttgaaa gagaagttga
    22201 agaagctaca gacttctaca gtgcagtaga tcaattagag gtagctacta aaggcttaga
    22261 aagaattgct gagaccttca actcttctga tgcggttgaa tttgaagctg tagcagaagc
    22321 cgaaacagaa gccgtaccag atgaagaaga agctcttttt gaggataatg acgaagcaac
    22381 tgaagcagga aaccaagaag caagcataga agaaacacaa acagaaacct accaacaacc
    22441 aaaaattgat gaaaaacccg aatcccagag tgataaagaa aattatgttt atgaagctga
    22501 aaaaatcgta tctgaaagtg atgtaccgaa aaatttagat gctgatttgt tagaaaaaga
    22561 ttttgaacag atcatagcca ataaatttca acctgtagtt ttagatgagg ttagtgatcc
    22621 tgttgatttg aacgaagaga tgcgatccat tgcagagtta aaaccattgc tgtttttagc
    22681 taaagagagc atgaacgctc gtatctctat tgctaataat aaaatcaaca gtgaattaaa
    22741 taaaacgaaa gagcaattgc tactcaaagg aaagcaaatg ttagcggatg agcttttgcg
    22801 gatagaggaa gcaacatcac taagtggtca aaatcagttt acaaatacgc tatcggtcgc
    22861 taaagaaagc tacgaagcag aagcatctaa aattgatgat ttagtgatgg aaaaatccag
    22921 tatcattcgt aaagattatg atatggcgaa acaggcttat ttggaagcag aactaaagaa
    22981 actagagcaa cggtacgatg ctgaacatct gccagaagtt aaacaacaag aagatgaatt
    23041 tagaaaagca actgaagaaa gtcttgatca acaataccaa gaagcaattg aatctattaa
    23101 aaacgatgct ttgaaacaaa atcaagaagc aaaagaagtt gtggtggaac ggatcatcaa
    23161 ttcattgaaa ccggaaattg aaaaatcttt aacagaagtg caagaaaaaa cgttagagat
    23221 tgtcgaaaaa acaaaggcag aaaatgaagc tgagttccac gatacaaaag agaaaacaac
    23281 agatgtggtt gtcaaatatt ttgcgaacaa agaagaagtt gaaaagctag tagctaaaga
    23341 tacgaatgag ttagaatcca aacttgttgc acagcaaaac attattgaag attataaaaa
    23401 tcgagtaaaa gagttcaagg atttagcaga tggctataaa ttaaatagcc aaaaattgac
    23461 tgaacaaagt gaagctttac gagaagattt atctcgtgca agaaacgacc tatcacaaat
    23521 ggcggaaaaa acaaagtcac tagtagttac tacagaaaat caaactgagt ctaaacgtga
    23581 ttttacaagc aaaaagatgg cgactatcat cgctgtatca gcaatggctt gtggaacgat
    23641 tttgggctca tcctacttca ttagtcataa tcaccaagaa gcccaagctc aaacagaaac
    23701 ctcaatttca agagtggaaa attcactagg agcaaagaca gcttctactt caacaacaga
    23761 caacaactat gaagcaacac caacgacaga atttaaagtc ggagaagatg ttgtcatcag
    23821 tattgatggg aaaactacta aggctgaagt actttccatt gacggtgata aggcagttgt
    23881 gaaagctcca gatgggaatc aatacaaaat acaagctcac ttaactgaaa agaattaagg
    23941 agggctaaaa tgcgagaaga aaatattgtt tatccagtaa ttgaaaatca atcaaataca
    24001 aaatcttctc aacttcttca agaagtgcaa gtcgtttatc cggttaatga actaccacaa
    24061 acgaatgaaa cagtgagcaa gtcaatatca ttaattggct tgcttattgt tgcaacggcg
    24121 ctaactgttg gtgtttatcg taagctgaaa gtgaaaggaa gtatttctga tggcagataa
    24181 ctcaaactat ggatttaaac ataatttgca tcaaaaacct tctgaaacaa tagtcagagt
    24241 tgattggctt gaaaaacatg tcaacagcac ctattctgtt acagcaaaaa gactaattgt
    24301 ttcagctatt gttgttgcag ttgttgttct tggttcaatc gtttatttgg tttccgatac
    24361 gattaaattg agatttattt ttattccaac gctattgatg ataattggtt ttcttatact
    24421 gattagtcag tatatgttta ttgatccatc tagcggatca ctgatctcag gctctattag
    24481 actgtttaaa tcagtagtta acacagcaag agtaacgatg gggaaaaaga aaccatttcg
    24541 aggaacggag attttgaaag ttagacaaga tggaacgatt atgtatcgag atggtagtgt
    24601 agcactaatg tttaatttgg atggtgctac ctcgttaacc gccttcccag aagaaatcat
    24661 agcgcaagaa ggcaaatcaa ttcaatacca aaatgcaagg gatcgttcct tggctgaaat
    24721 caaaattact tcatcacaac ctcagaatac ggaacgtcaa atacggaaca tgaaagtaca
    24781 aaaaagaaat gcaaagagca acaaagctgt gtttgacata gtagatcaac aagagaggtt
    24841 tgtatctgaa tatattgatg gggtcaaaac tacggtgatc caatatctta ttttgaagtc
    24901 acaatctcag atggtattgg atgaaggcgt agaacgttta gaaaattaca cgagtagagg
    24961 gctttactat agcgttactc agttgtcagc taaacaaacc aaagaaatat tatcagaaat
    25021 ttatgcactg aaataataga ggtgagaaat ttaatggaac taatgagtag atcgaagcta
    25081 cttaatttca gaaaaaagaa acacaaaaaa gagcaaaatg ctgatagttc taaagcggtg
    25141 aataatactt atgtcagtga tttaacatca ttggttggtt atcaatttac accatcttac
    25201 attaggacag gtaatcggtg gggaacgatt gttaaattag tgaatacgta tggaatgaac
    25261 agaaattatg aatttggatg gatggttaaa gctattcctg aagtgaaggt tgaaggagta
    25321 aaagcttact taatcgaagc ggataagcca gtttcgcaaa aagatcagca agaaatcatg
    25381 cacgaaaatg tcggtggatc aacgaacagt tatgaaaata acttgaacga taaaaattca
    25441 gatgcagaaa tcaataaaac tgttatgcga atcattgatt tgaatacagc atctctgctt
    25501 gacagtacgg caaacaaaat tattgattgg aagttagaaa tcctcttagt atccgattct
    25561 cctaacaaga tcagcgaaca gttgatcaaa ctgcaacgcc tgtatgatga aaaaatgtat
    25621 ggattaaaac ttatgtcttt tggtggcgat caacgtgatc tgtttgatag gcttttaagt
    25681 gagccagtcg gtagtgttta cgaatacaca tctatgagtt ctgattttgc aggaaatgat
    25741 cacgctatcc gtaaaggctt agatgatgca gacggatggg ctatcggaac gatgtctaca
    25801 agctacgctt caggtgtagc gtttatggca atggatcgtt cttttaaggg taaagtctta
    25861 gtagcatccc acaaagatgc aacaatcaaa ggctatagcg aagaaatctc agcatcatca
    25921 ctttgggggc aattgattgc taataacgca atggcaaatc ataaacggac gtttcacatc
    25981 gtattaaata aatttgacta ctttggtgat ccgacagggg aaacatttgt tgctaagcca
    26041 tctttaaata gtgtgataga gaagattaat ctagctgaag aaggacttaa ttctttagag
    26101 ctttttggtg atcgaaagga tattggagat ttatatagac gtaatcttga taaactaact
    26161 cagattttct atctgttagc ggacagagaa ttaaatcctg caacaagagt attgctgaag
    26221 aaagagttgg ggaactttta tattagtcag aagctttggg atattaacta tgaagagcgg
    26281 tcgaaactgt tagtaaacag cagtgataca atgccggttc ttggggattt catcagctat
    26341 ttaacaaatg ctgagacaaa agcgaaaaaa tccggtacag aaaaagaaat tgatcggatt
    26401 attgtattaa aagatacatt gcgaacagca ttggatagct tctctaagat gttcaatcag
    26461 agaactacat tgaaagaaat tggcaagagc gacaaattgc agttttacta caatttgagc
    26521 gatttaagaa cagaaatcaa tgtcatggaa gcccaatttt taaatacctt tgacttcatc
    26581 acgtcacaag ctaaaaaagg cgatatcgtt atgatccatg ggtttgatag attatcagtt
    26641 gaaaccgttg gattaattaa aaagcgacta acaatgcttg aagaagatgg agtccggctt
    26701 gcgtttctct ttgatactat cggatcagga gaatctaaag acggtatcga aacagctgat
    26761 attttcaata cagatggaaa aatttatcaa agttttgaaa gtgattttaa ctacaccatc
    26821 atcgggaaaa tgaccagtga agaattgatg aagtatgaaa agaaggttgc tcctgcaaga
    26881 aaaggagaag atgatcaaaa actatcagtg gatttgagac agtatttgac atcaggtcga
    26941 agcaataaat ttcaaatcag aagaccagcg gatttgacta ataattttgt tattggagag
    27001 tttattattt aagtcttatc cttgtttttg aatgtttaag gataagaaac aatatttata
    27061 tacatgactg agagaaagaa ttatagtggc ggtaaaataa tgaaatcaaa tataaaaata
    27121 atagcaggtg ataaaatgaa ggaaaaagaa aatttagttt ggtattttaa cagaaatccg
    27181 catatgctga ttacaggaag aacagctaca ggaaaaactt atctgttgaa aagaatggct
    27241 gactatttat cgcaagttgg tattgtttct gcttcttttg acaaaactga atttgaagaa
    27301 attttatctt cgtctcgaaa ggaaatggaa gatcggtttg agtatattaa agaaaatcgt
    27361 tttaaaatgt ttgacgtaag ttactctgat ttagacatga agccttattt tgttctgata
    27421 gatgagctag attcaaggtt gttactctta gaaaaagaaa atccattggc ttataattac
    27481 ctattactaa taataaagga tattgtgtta aaaggacgag tagtaggtgt tttttttatt
    27541 gccgttcgtc aaggaaagca accattgtta gaatctatag aagataatat gttaacacgt
    27601 atttttttag gggattccaa aggaaatgga acgatttgtt ttgatggaaa tacaaagaaa
    27661 ttccaaacag agctagtaac agaagatttc ttttctgtag actgttagta tagcggaggt
    27721 taaataatga aaccaaacat aaaaatttta gatcgaatat ttctaggtaa agacacagaa
    27781 gtaatcctaa ttcaacatga a
//
LOCUS       DAZUMO010000033        24916 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000033 DAZUMO010000000
VERSION     DAZUMO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.033"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..1491)
                     /locus_tag="ACG8ER_001479"
     CDS             complement(join(152..1050,1050..1491))
                     /locus_tag="ACG8ER_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS5050068.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            1788..2435
                     /locus_tag="ACG8ER_001480"
     CDS             1788..2435
                     /locus_tag="ACG8ER_001480"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5050069.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            2718..2791
                     /locus_tag="ACG8ER_001481"
     tRNA            2718..2791
                     /locus_tag="ACG8ER_001481"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2752..2754,aa:Ile,seq:cat)
     gene            3339..4313
                     /gene="bsh"
                     /locus_tag="ACG8ER_001482"
     CDS             3339..4313
                     /gene="bsh"
                     /locus_tag="ACG8ER_001482"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5050070.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            4638..6533
                     /gene="asnB"
                     /locus_tag="ACG8ER_001483"
     CDS             4638..6533
                     /gene="asnB"
                     /locus_tag="ACG8ER_001483"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS5050071.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(6728..7174)
                     /locus_tag="ACG8ER_001484"
     CDS             complement(6728..7174)
                     /locus_tag="ACG8ER_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5050072.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            7402..7551
                     /gene="rpmG"
                     /locus_tag="ACG8ER_001485"
     CDS             7402..7551
                     /gene="rpmG"
                     /locus_tag="ACG8ER_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5050073.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            7639..8157
                     /locus_tag="ACG8ER_001486"
     CDS             7639..8157
                     /locus_tag="ACG8ER_001486"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5050074.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8234..9190)
                     /locus_tag="ACG8ER_001487"
     CDS             complement(8234..9190)
                     /locus_tag="ACG8ER_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5050075.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            9356..10162
                     /locus_tag="ACG8ER_001488"
     CDS             9356..10162
                     /locus_tag="ACG8ER_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050076.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10159..11148
                     /locus_tag="ACG8ER_001489"
     CDS             10159..11148
                     /locus_tag="ACG8ER_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5050077.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11145..12149
                     /locus_tag="ACG8ER_001490"
     CDS             11145..12149
                     /locus_tag="ACG8ER_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5050078.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            12281..12823
                     /locus_tag="ACG8ER_001491"
     CDS             12281..12823
                     /locus_tag="ACG8ER_001491"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS5050079.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            12843..13541
                     /locus_tag="ACG8ER_001492"
     CDS             12843..13541
                     /locus_tag="ACG8ER_001492"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5050080.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            13562..13774
                     /locus_tag="ACG8ER_001493"
     CDS             13562..13774
                     /locus_tag="ACG8ER_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS5050081.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            13774..14736
                     /locus_tag="ACG8ER_001494"
     CDS             13774..14736
                     /locus_tag="ACG8ER_001494"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS5050082.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            14754..15149
                     /locus_tag="ACG8ER_001495"
     CDS             14754..15149
                     /locus_tag="ACG8ER_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5050083.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            15312..15677
                     /locus_tag="ACG8ER_001496"
     CDS             15312..15677
                     /locus_tag="ACG8ER_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS5050084.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            15674..17485
                     /locus_tag="ACG8ER_001497"
     CDS             15674..17485
                     /locus_tag="ACG8ER_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS5050085.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(17547..17714)
                     /locus_tag="ACG8ER_001498"
     CDS             complement(17547..17714)
                     /locus_tag="ACG8ER_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS5050086.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            17963..18952
                     /gene="galE"
                     /locus_tag="ACG8ER_001499"
     CDS             17963..18952
                     /gene="galE"
                     /locus_tag="ACG8ER_001499"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS5050087.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            18964..20448
                     /gene="galT"
                     /locus_tag="ACG8ER_001500"
     CDS             18964..20448
                     /gene="galT"
                     /locus_tag="ACG8ER_001500"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS5050088.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            20472..21452
                     /locus_tag="ACG8ER_001501"
     CDS             20472..21452
                     /locus_tag="ACG8ER_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5050089.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            21643..22365
                     /gene="tsaB"
                     /locus_tag="ACG8ER_001502"
     CDS             21643..22365
                     /gene="tsaB"
                     /locus_tag="ACG8ER_001502"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS5050090.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            22349..22891
                     /gene="rimI"
                     /locus_tag="ACG8ER_001503"
     CDS             22349..22891
                     /gene="rimI"
                     /locus_tag="ACG8ER_001503"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5050091.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            22898..23362
                     /gene="rimI"
                     /locus_tag="ACG8ER_001504"
     CDS             22898..23362
                     /gene="rimI"
                     /locus_tag="ACG8ER_001504"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5050092.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            23359..24375
                     /gene="tsaD"
                     /locus_tag="ACG8ER_001505"
     CDS             23359..24375
                     /gene="tsaD"
                     /locus_tag="ACG8ER_001505"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS5050093.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         7906 a         4218 c         5120 g         7672 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgtg aactgaaccc caaaagttga actatttaat ggactgtttc cgatattcta
      181 ctggagatag tccatttaac tttagtttta tccttttgtt attataccac ctaatatact
      241 catttaattt actttgaaat atctcaattg atcggaattt ttctcgataa aaaaactctg
      301 atttaagcac accaaaaaaa ttttctatta cagaattatc taagcaattt ccttttctag
      361 acatgctttg aataatgtta ttctctttta attttttttg atattgtggc atctgatact
      421 gccatccttg atctgaatgt agaatcagtg aaccctcagt tccctttttt ttaattgctt
      481 gttgaagcat ttcttcaatc agtttatatg ttggactagt tgatatacta taactaatta
      541 tttctccgtt aaataaatca agtataggag atagatagat ttttcttcct tttatcttga
      601 attcagtgac gtctgtcacc cattttttat tgggtgtatc tactgaaaaa tttcgtttta
      661 acacattctt ggcaattttt cctactgttc ctttataaga tttatatcgc tttattcgga
      721 tttgacaggt aagtcccatt tgggacatta attttctaac tgttttatga ttgattgtat
      781 atcctttcat ctttaacgct aaagtgactc tacgataacc ataagagttt cttgattctt
      841 ttacaatcgc tgtaatttct tgctttatct tgctatattt atctggctta tctaattttt
      901 ttacccagta ataataagtt gacttcgcta attgtgcaat tgaaagtaat agattcaatt
      961 taaattcttt tttgagctga aggattgttt taaccttgat ttcttcttgc tcaaatcttg
     1021 ttcttgaatc aaggtttcta acttttttaa atatgcgttc tctgctctta aacgaataac
     1081 ttcttcttca agagactcat ctttaagttt tttaggaatg tttagcttgg aattcatact
     1141 aatttttctg cccctttttt ggctctcaag tgaagaagca ccgccttctt catattgctc
     1201 tatccattta cttagagttc tatttgaacc gatattaaat tttttagcag tttcttggat
     1261 agaaagacca tttgtttcca tatattctat aacatcaagt ttaaattttg tagtgtagct
     1321 tttgccacct ccaaccaagc cttcccaacc atgatagtta taaatcctta cccaatgtct
     1381 aaccagtgta cgatttattt gatatttatg tgcaagatat ttgtagccgc cttcgttatt
     1441 taaatagtct gaaactactt ttttcttaaa aacaaatgta tatttccgca aaaaaagcac
     1501 cccttttaat tagatttcta gtctaacttt tggggtgcac atcatttttg aggggaagta
     1561 cataatgcaa atttgtccat aacttattga ttatcttgta aaaatgcggt aatttaatag
     1621 ttagtgagaa gaggtgctta attatagtta aaaaagagga aatagaagca gcatgtaacc
     1681 aagcaaatgt taggtttatt ggagaagctt tatctggata tcctaagtat aaatacttac
     1741 acaactggtt acaaagtaaa agtttttatc ttaataaaga aagatgcatg cctaataaaa
     1801 attatagggt ttataaaaga ggaactattg tatatgttga ttttggggtg aacgtgggtt
     1861 atgagttatc tggtaatcat tttgctattg ttttaaataa taaagataat tcgaaaaatg
     1921 gtgttgtatg tgtagtacca atctcttcaa aaaagaaaag taattatgta tctgtaggaa
     1981 aaatattaga agttgctagt attaaacaat ttataagcca agctgataaa ttaaaagaga
     2041 aacttaggat aattatttta ctttcattaa atagaggcat cattgatgaa agcgatttga
     2101 atagttttat taaagatatt gaagctaaag gtgaaaagct ttcagtggaa gaagtagaaa
     2161 aacgagctgt aaaacataat ttggtttgcg agacaaggga acaagtaatt aaagaaataa
     2221 aatcattatc tgaagatatg gtaaaatatc agaaggtgtt taattgctac aagaaatata
     2281 cacaagaatc ctatgtaatg ccacttaata tccaaacgat tagtaaaaat agaattagaa
     2341 gaattaacaa gtttgatcct tctggaaata tgaaggcacc tggaaatgtc ctcaatgaag
     2401 tagatgaaag tttaaaaaaa tattttacaa agtaaacgat atttttgttg acatttttct
     2461 gttcgttagt taccatgtag ttatagaagg acgtaagcgc ctatttttaa tgtacacatc
     2521 gggacgtatg cgcccacata gaaacatagt tgagagacat ctatatggtg tctctttttt
     2581 tattaatatg caaaaaagtc tagttttttc aataatattt atgtaataat aaattagcca
     2641 ccacaacaaa gaatgaaacc cattattatc tagtctatgt ccattctttt tgtttgcagt
     2701 agttgtgatg gctttccgta ctcttagctc agttggttag agcagacggc tcataaccgt
     2761 ccggtcgtag gctcgagtcc tacagggtac atactctaag tgatttaaat tgcgaataaa
     2821 atataaaaat gactcccctt ttgactcccc caagcagtat atggatgtct ctaaaagttg
     2881 tttttttaga taataaaaag ctgataataa gcgaaaaaca cttaaaatca gctttaaacc
     2941 ctattctcga aatccggcga accggctaat accggcgcgc aggttcaaat cctgtacctt
     3001 ccttatatca acgattagag gttgtgaaga agctgtcctg catcaagtaa taagaacggc
     3061 caaacaaaaa tagcttttcc tattttttcg tttggttggg cttattaccg cagatgcaag
     3121 cttttgaaca ccgtttgttc ggaataaggg gctgtgacaa gacttttgtc acagcccctt
     3181 attgttgtac ttttaaccta caatattttt tgattccccc tgagtactat tttttataaa
     3241 tgctagtaaa tcaatcttct ttggcctttt ttttgataaa aaaaaatact agatgtaaga
     3301 taaaagtgta aaaacgattt gttttaggag ggaaaacgat gtgtaccgca atcacttatg
     3361 caacgaaaga ccattatttt ggaaggaatt ttgattatga gatgtcttat aatgaagtag
     3421 tcactattac tccaagaaat tatcgtttcg attttagaaa ggtaaaaaat ttagataaac
     3481 attatgcaat gataggaata gcagctgggg tatccaacta tccactctat tatgaggcta
     3541 caaatgaaaa agggttgagt atggcaggac tgaattttcc tggcaatgct gattataaag
     3601 aattacaaga aggaaaagat aacgttgcac catttgagtt tattccatgg atactagggc
     3661 aatgctcaac catagacgaa gctaaagaat tattggctac tatcaatcta gtcaatatcg
     3721 attttagtga aaaactaccc ttgtcacctt tacactggtt attagctgat aaagaaaaat
     3781 cgatagtaat tgaaagtatg aaagatggac ttcatttata tgataatcct gtcggtgtgt
     3841 tgacaaataa tccgccattt gattatcaat tatttaatct gaacaattat cgctcgctat
     3901 caaatggaac accagaaaat catttttcaa accagatcag cttgaatgtt tatagtcgtg
     3961 ggatgggagg attgggctta cctggagatc tctcttctgt ttctcgattt gtaaaagcca
     4021 catttactaa aatgaatgct gcatcaggtg attcagaatc tgaaagtatc agtcaatttt
     4081 ttcatatact aggctcagtc gagcaacaaa aaggagtatg cgatactgga gaaggaaaat
     4141 acgaatatac gatttattct tcttgctgta acgttgataa agggatttat tattatcgta
     4201 cgtatgaaga cagtcaaatt actgctgtaa atataaataa agaagacttg gatcgtcatg
     4261 aattggtcag ttatccagtc ataaaagagc aacagataaa ctatgtcaat taattaatga
     4321 atagatccgt ttggtatcat attttctgat acagtcataa ttagacacga tctttaggtc
     4381 gtgtcttttt ttgctgtttt ttttggaaat agcatccact aatatttttt taacacgaag
     4441 agaataaggt agcctcaaaa aagaaaacat ggtaaattag atattgggac tttattttct
     4501 gtaaaaacag aaactttcat cttttgtttt cacgtgacga aattgtaaga gagaaaatat
     4561 tcgtccgcta tttattctat gataagatgc atatgaatga aataaacagt aattttatat
     4621 taggatgtga aagttaaatg tgcggtattg taggttttgt taacgacaag gacaacaaaa
     4681 aaacaattat taatacaatg atggatcgaa tcgttcaccg tggacctaat agttcaggag
     4741 aatatatcga taaacatgtt gctttaggat tcagaagatt aagtattatt gacttagagg
     4801 gcggtactca gcctatttac aatgaagatc ggacaaagat cattattttt aatggcgaaa
     4861 tctataatta ccagcctttg agagaagaat tgattgcagc tggccacgtt tttcaaacac
     4921 atgcagatac agaagtcctt ttgcatggtt acgaagaatg gggaacagaa ttattgcaaa
     4981 agatccgcgg aatgtttgct tttgctattt gggataatga aaagaatgag ttattcggtg
     5041 caagagacca ctttgggatc aaaccatatt attatgcaga aatgaacgga acatttatgt
     5101 ttggttcaga aatcaaaagt tttctgcctc atcctgattt taacaaagaa ttgaacaaag
     5161 aagcgttgaa accttatatg actttccaat attctccatt aaacggcgaa acattcttca
     5221 aagatgtcta tcgtttgcct gaaggccatt attatacgta taaagatgga aaactagaca
     5281 ttcagcaata ttgggatgca gattttgaaa caaaagaaac gcattcccga caagaatgga
     5341 tcgaaaaaat cgacgaaacc gttcaagctt ctattgaagc acatacggtc agtgatgtag
     5401 aagttggttc tttcttatcc agcggggttg attccagtta tgtaacctct gtattgaaac
     5461 cagatcattc tttctcaatc gggtttgatg ataaaaccta taatgaagcg attgaagcca
     5521 gaaaactaac cgaactattg gacttagata atacagcagc agtgatcgac ggagacatgt
     5581 cgttcaaagc attccctttg atccagtatc acttagatga acctgattca aatccttctt
     5641 gcgtaccttt gtacttttta gcaaatcttg cttcacaaag tgtgcgggta gtccaatctg
     5701 gagaaggggc ggatgaactg tttgcgggat accaaacgta tggattccat acgaattcaa
     5761 aattcatccg tgtgattgct caaggattga aaaaattacc aaaaggaaca cgctataatt
     5821 taggacgtaa aatcggaaaa atgaaaaatt tccatggacg gatccattta tatgaatcac
     5881 ttgctcctgc aaaagaatat tttatcggtc atgctcgagt gttcgaagaa tcagaagcat
     5941 cagaagtgtt aacaccaaaa tatcaaacag caccatctgt ggatgaaatc atgactgttc
     6001 attacgagaa aacagaagga atcaaagatg aagtcaataa gatgcaatat gtcgatcttc
     6061 atcaatggat gccaaaagat atcttgttaa aggcagataa actttctatg gcaagttctt
     6121 tggaagtccg agtaccttta ttggatatcg aagtcatgaa attggctcaa caaataccaa
     6181 gtaagttttt attgaatcaa aacaacacaa aagatatttt cagacaagct gcgaacaaac
     6241 atcttccgga agaatggtcg aatcgtgtga aattaggatt ccctgtgcca atcaaagctt
     6301 ggttgaaaga agaacacggc tacgaacaag tcaaagcatt attcgaagca gattttgcaa
     6361 aagaattttt tgatcaagaa aaaatcatga agttgctaga tgaccatcac gaaggacgta
     6421 aagaagagca aagaaaaatc tggacaatct tcagtttctt gacatggtat aaagtctact
     6481 ttgtagatga aacgattccg caagcagaag caatcgacta tgtgactgtt tgatgaaaat
     6541 caaatatcaa taaaaaagag gttttctcct accgtttaac actgagtgat aagaaacaat
     6601 ttcaaaaaac agctcttccc gttttataga tattatgact tatggttcgg agttggcaga
     6661 ggaacaccat tgtttcgaac ataagggggt tgtgacacga ttttcgtcac aaccccttta
     6721 catatgttca ataatcgatt tctttttctt ttgtcccatc ttttttataa atgaacaaag
     6781 ttgattcttt gttttgtgct acttgctttc ctttttcgat tgcttctttt tttgtatcaa
     6841 aatggttgct tgcctttttt gcccctttag atttcacgat ccattgttct tcctcatatt
     6901 ctacgatcac atctgaatca agcagttttc cagcacgagg attcgtatcg tgttcatccg
     6961 tcttgcttgg atttttttct ttttcgaaag ttcgtttttc tgattcacta gcatcttcat
     7021 accatttttt tgcttgatca atggcaattg ggatcgcacg gttatcaggg tacccttcat
     7081 ctaataatgc atttgcgatg tcgattgctt tttttcttgt caatttatct aagtttttca
     7141 ttgatgcagg aaagtccttc atgttccatg gcatattagt atcccccttt attaaaaaac
     7201 ataatctata taaacaagtg taagggtatt tatacaaaaa gaaaaagatt ttgtttgaga
     7261 agtaaaaccg ttgtatttat cgtcaagtag ttttacttaa caaaatcgtg aaaaacacta
     7321 gcaatctaga aaaaatcatg gtaagataat caagtctgag agtcatctta gaggattctt
     7381 gtaaaaatgg gagggaaaaa catgcgcgta aacatcactt tagagtgtac ttcttgtaaa
     7441 gaacgtaact atctaacaag caaaaacaaa cgtaacaatc ctgatcgttt ggaaaaacaa
     7501 aaatattgcc cacgcgaacg caaagttact ttgcaccgtg aaacaaaata atgtttttaa
     7561 agttctcaat tccttgctgg tcaaagaatt gagacttttt ttatctcaaa acatctaaaa
     7621 tcataaggag cggataatat gtttatttcc atgtgggcac aagataaaaa tggtctgatc
     7681 ggcaaaaatg gtttgttacc atggcgattg ccaaatgata tgcgtttctt tcgcgaacat
     7741 acaatggatc gaattttagt gatgggaaga aagacctatg aaggaatggg ggatctttca
     7801 cttccttatc gccatatcat cgtgttgaca acgcaaccaa attttcgcac gaaagaaaat
     7861 gcggaagtaa tgcattcgat cgatgaactt ttgtcgtata gtcaaacgat ctcagaagat
     7921 atctatgttt cgggtggtag tcggattttt caagaacttc ttccttacac tggtcggatc
     7981 tggcgaacat tgatcgatag cacgtttgaa ggcgatacgt atattgggaa tattgatttt
     8041 tcagacttcg cccttataga ggagtatgag gggataacgg atcaagaaaa tctatatgcc
     8101 catcgttttc aaaaatggga aagagtaaaa aatagggttg cttcaaaaga agaataaaat
     8161 ctatgcgtct agtttgttaa taagaaaaaa agcatctcac gactaatcgt gaagattctt
     8221 tttttagtta aaatcaattt agtttttctt tgatatcatt gaccatttcc tgactggcaa
     8281 taggtccgtt atatagccaa ctagattcag gtccgaattg cagtacatga ttgtctgtca
     8341 ctgctttaag attcttccag cttggttcat tgaacatggc agcatctttg tcactgttca
     8401 caaggatgat gtagtcagca tctaattcag ctagcttttc agaggaaaca gcagaccagt
     8461 cagaagtggc ttttttggag acctcttcca ctagatcagg aacaccaaat tccagatcgt
     8521 cgtatacgat tcttccactt gaccgatttt tgctgaccat aaaggctgag ttgttggtca
     8581 cccacaacac tgcagctgtc ttgttctcga ttttatctgc tagttcttct cgagttttct
     8641 tgatggattt ttgataatcg gaaatgattt tttctgcctc tttttctttt gccaaagctt
     8701 tgccgacatc ttttagttgt tcttcccatg tgacatctgt tccgtttttt acaacataag
     8761 ttggcgcgat tttgctatat tgttcgtatt tcccgccttc gacagtagcg gaagaagaaa
     8821 tcaacagcaa atcaggctca aagcttaaca ctttctcata aggaaggtca taagaaatcg
     8881 ttgggacatc ctttaaatca tcttgcagat aatcttgaat ggaaccactg ccaacagtcc
     8941 actgggcgat aggtttttcc cctagtgcaa tcagatagtc ttctaagtaa ctgccgatga
     9001 tccgtttggg cgcgtttgga atctctactt tatgtccaag tgtatctgtg actgtatgag
     9061 tactattgga tgaagtcgtc gaattggcag ttgtagctgt ttctttatct gaagaggggg
     9121 agcaagcgct cagtactcca gccagcagca atatgctaga taatgtgaat acttttgttt
     9181 ttaatttcat ttctttcctc ctaacataat tgagaatctt tctcattggt tattattcct
     9241 gatatttcga acgctgtcaa ccgcttcatt tggctataga tatgaaagta gcgcttgacg
     9301 aaatgttgaa acagcgtcat aattgagaat gattttcagc gaaaggatgg gtgaaatgac
     9361 gagattaaga gcagctgaca ttgcagccgg ctacgaaaac aaaaaaatca ttgaaaacat
     9421 gtctgttacg attcctgaag ggaaaatcac tagtttgatt gggccaaatg gaagtggaaa
     9481 atctacttta ttgaaaagtt ttacccgtat actacctgtg aaatcaggaa agatcattgt
     9541 caataactct gatatctcta catacagttc aaaagaactt gcaaaaaaaa ttacattatt
     9601 agcacaaact agtgaacacc ctacaggtat gacagtagaa gaagtagtat cttacggacg
     9661 atacccttat caaaaactgt tcagtggcat ggacacgcaa gatcatgaag caatcgagtg
     9721 ggcgttggat gctacaggtc tagctggatt tcgtatgcgg gatatcgcaa cgctgtctgg
     9781 tggacaaacc cagcgtgtct ggatcgctat ggcgcttgct caggaaacag atatcttgat
     9841 cttggatgaa ccgacaacat ttttagatcc ggcacaccaa ttagagatcc tacagttatt
     9901 gactgaaatc aatcaaatga aacaaacaac gatcttgatg tcgattcatg acattaatca
     9961 tgcttcacgt ttttcagatt atttattagc aatgaaaaac ggtaagttga tcacagaagg
    10021 tacacctgaa gaagtgatca caaaagagtg tatgaaaaaa atatttgaga ttgatgcaac
    10081 gattgtcacg cttccaagaa caaataaacc agtttttcta acctatgatt tgttgccaaa
    10141 agagcaggag gatcaattat gaggactatg aagaaaaata gactcctgct tttggtctgt
    10201 tttgttcttt tgattatttt ttttgttgct ctttcaaatg gcgcagcgac tgtgggaaaa
    10261 gcagattttt ggcaggcgtt gtttgatttt gatagatcaa aacaaagtca ccaaatcata
    10321 cgaaatattc gtttgcctcg tgtggcggga gcatttcttg tagggagttg ttttgcattg
    10381 agcggtgcat tgatgcaagg agtgacaaga aatcctttag cagattcagg cttactagga
    10441 atcaacgcgg gtgcttcgtt ggcaatggcc cttagttttg ctttttggcc taaagcttct
    10501 gatttttctg tgtttctaat gtcattggca ggttcttttg ctgtaacttt gctcgtattt
    10561 ggttttttgc agttttcgaa aatgggcatg aatcctttac agctgatttt agctggcgta
    10621 gctattagtt ctttttttac tgctatcagc caagcactta cccaattctt taatctgcag
    10681 caggatcttg ctttttggtt tgtaggcgga gctgccaata tcacatggaa acaattagca
    10741 gtattgtttc ctttatatat acttgctgtt ttggtttctt tttcattagg aaaagcaata
    10801 tctttgattc atttgagtga aacacatgca ttagctcttg gaagtcatcc taaacgaacg
    10861 cggttgattg ttttctgtct agttgcagtg ttagcagcca ttgccgtttc gttagtcgga
    10921 cctgtttcat ttattgggct gttggttccg ccagttgttc gcggaatggt tggaacggac
    10981 tatcgttatg ttctacctgt ctctttttta gttggcggtg gattggttat gttggcggat
    11041 tttacagcga ggatgatcaa tccccctttt gaaacacctt ttggattagt cattgcattg
    11101 attggcgttc cgtttttact gatacaagta aggagggaac aacggtgaaa aaaatatatt
    11161 ggacgttcta tttcttgctt tttgtacttg tcatcctgtc tataggtagt ttaatgattg
    11221 gtacgccttt tatttacctg gatcagcttt ttgccacgtt catagggaaa gaatcatctt
    11281 ctttggcgct gattgttaga gaatttcgag ttcctcgatt actgatcagt ttattagcag
    11341 gagcttgtct tggcgtttct ggttatatct tgcaaggagt aacaagaaat gaattggcag
    11401 attctagtat attgggcatt aatgccggag caggattttt agtgatgctt tatttaggtt
    11461 tcttttcgca ctattcgctg ccgtttctat tgctagttgt agcctttcta ggtggtgttt
    11521 tagctgcttg ctgtgtctat cttgcggcct atgatcgaaa tggctttttg ggtatgaatc
    11581 gaatcttatt gtccggtatt gccgttaatg ccggcctttc tgcattaaca ttgctatgca
    11641 cgatccaatt atctaaggaa aattatggat ttgtgaatgc atggttagcg ggatcaatct
    11701 ggggtgcgag ctggccttat gtttgggcat tgcttccatg ggcggttatc ttaattccgc
    11761 taggaggact ttatgcacag aggattggat tgctatcatt tggtcaagaa cgcgcgcaag
    11821 gattaggcct taacgtggct aaaagtcaaa aaatcctttt gttgttagct gttgctttgg
    11881 caagcggtag tgtagcggtg acaggtagtt tatcttttgt aggactacta gctcctcatg
    11941 ctgcgaagct agtcgtacgt aaagaaaata actggacctt tgttctgagc ggtttgttcg
    12001 gttccatttt tctgcttagt gcagatatgg ttgctagagt gattctacca agtggggaag
    12061 tacctacagg aattttgatt gccctttttg gtgcgccata ttttctgtat gcacttttta
    12121 tcagacaaaa acacgcgctg aacagttgaa atttattaaa aaaggaaggc caatcatttc
    12181 taggtcttct ttttattcta tttatgcatt tatttgaaag ttcgattgct atttttcttt
    12241 ttttcctata ccattttatg agagaaggaa gggtgagttt ttggaccaat tgaaagtaag
    12301 attacgccgg aaaggaatcg aaaagctgaa aaatctggcc aaaagtggca agaaaaagga
    12361 aaaagaacag cgaatctata aacagctatt cgccagcgag tactggaagt cagcaaaagt
    12421 gatagggacg actttatcga atgaatttga actggataca cagccggtta tccaacgagc
    12481 agaaaaagaa caaaagaaaa tagttgtacc taaaactttg ccgaaacgtc agatggcgtt
    12541 ttatgaaatg gatgaacagt ccgttcttgt acgttcgagt tttggtgttc ttgagccaca
    12601 atcagagaag ctctatttgc cggagatgat cgatctcctc attgtaccag gtattatcta
    12661 caatagagcc ggttatcgaa tcggttttgg cggtggttat tacgaccggt atttaaataa
    12721 ttttcataaa aaaacatgta gccttgtttt tcgtgaacaa ttgaatgaac aatggcaacc
    12781 ggagagcttt gatcagaaga tccaacaatt gtttattgac taaaatgaaa tgggaggttt
    12841 tcatgaatta tcaacagcaa ataaaattgc gtgcatggtt aagacgaccg ttatggacct
    12901 atgctttttt agggatacag acagcggtct tcctcatgat ggaattattt ccgcgacttg
    12961 agatccctta ttatgccgga atgtatggtc cttatcttgt tcacttcaat gaatggtggc
    13021 gtttagtgac accaatcttc atccattttg gactgatgca tttcgtaatg aactcactga
    13081 tactgtattt tatggggcag caaatcgagg caatctatgg ccattggcgt tttttcttga
    13141 tttatatgtt cagcgggata atgggaaata cagcaagctt tgcttttaac gaagcgaatg
    13201 ttctgtctgg tggtgcgagt acttcgatat tcggtttgtt cggtgcgttg tttatcttag
    13261 ggtttcattt taaacataac ccagcgatcc agcagctagt cagacatttt cttttattta
    13321 ttgtgttgac atttgttttt ggtcttctgg atacctctgt agatgtttgg ggacatgtcg
    13381 gtgggctaat tggcggatta gtgttaggga atgttttggg tctaccgaaa caacagacaa
    13441 gctattcgat acaccaaagg attctttcta cactagtttt tgtatttctt tttgtaatat
    13501 gtattttact gggtttaaaa aaatatggtt tacttgtata atatgaagga agtgcttaaa
    13561 gatggagagc ttatatgatg tacagcagct gttcaaaaaa tttggcatct ttatctatgt
    13621 gggtgcacgc atctatgaca ttgaattaat gactatcgaa ttgcgtaatc tgtatgatag
    13681 ccgtttgatt gatcatgata catacatgac agcgtggcgc atattaaaaa gagagcaccg
    13741 aattgaagaa agccgaaaga aaggaaattt gtaatggaca agaagattat tgggattgat
    13801 ttaggtggaa caacagcaaa atttgcaatt ttaacaccag aaggagagat tcaacaaaaa
    13861 tggagcatcg ataccaatat tttggatgat ggtaagcata tcgttcctga aatcattgaa
    13921 tcaatcaacc atcgtttgaa tttatataat atgaaagccg aagactttat cggaatcggc
    13981 atgggtaccc caggaagcgt ggacagcgaa gctggaacgg ttattggtgc gtataactta
    14041 aactggacag aagttcaatt tgtgaaaaaa ctgatcgagg caggaacagg aatcaaattc
    14101 gctatcgata atgatgcgaa tgttgctgct ttaggtgaac gatggaaagg tgctggagaa
    14161 aacgatccag acgttgtatt cgttactcta ggaacaggtg ttggcggtgg tatcgttgct
    14221 ggcggaaatt tgattcacgg cgtgagcgga gcaggcggag agatcggcca tatcactgtg
    14281 gatcctgaag ggttcgaatg tacttgcggt aaacgcggat gtttagaaac tgtgtctagc
    14341 gctactggtg tggtacgtct aggtcgttac ttggcagaag aatacgcagg agattcaaaa
    14401 ctgaaagcaa tgttagacaa cggtgaagaa gtaacaagta aagatatctt tgaaatggct
    14461 caagaagacg atccgtttgc attgatggtt gttgatcgcg tatgcttcta cttaggttta
    14521 gcttgcgcca acttggggaa tacattgaat ccgtcaagta ttgtgttagg tggaggagta
    14581 tcggcagcag gagaattttt acgtagccgt gtagagaaat attttaaaga atttactttc
    14641 ccgcaagtaa gagaatcaac aaaaatcaaa ttagcagagc ttggtaatga agctggtgtc
    14701 attggtgctg cctcattagc cttgaaattt gcttaaaaaa acggaggcat tacatggttt
    14761 tatgggtaat taatatcatt ttgctattga ttgtgcttgc aatcatcttt tgggaagtct
    14821 atctacgagt aatggccaag cgttcagcaa cgactttgac agaagaagaa tttcgagaag
    14881 gaatgagaaa agcacaagta atcgatgttc gcgaaaaaga tgtgtttgat gctgggcata
    14941 ttttaggtgc aagaaacatc ccttacactg ttttgaaaga ttcattagga tctattcgaa
    15001 aagaccagcc tgtctatatt tacgatcaaa agaaaagctt gagcatccgt acagcaaata
    15061 aattacgtaa agcaggttat caagagattt atttacttaa gggtggatat gacggatggt
    15121 ctggaaaaat caaaagtaaa aaagtataaa acagatgatt gtatgacagc tttaaaaacc
    15181 gaatcaatca ttagattttg accaagatca catgaacggc gcaatggata gttgtgccgt
    15241 tcattttttt tattttgtgc tattatctac atatataagc taagcgaaaa ggcagaaaga
    15301 aggaaacaac tatgatcaaa ttaaaacgtg tttaccttga tgctgaaaaa gaggatggct
    15361 atagaatact cgtggatagg ttctggccac gaggaatttc aaaagaaaaa gcgcagttag
    15421 atctgtggtt gaaagaaatt gcacctagta atgaagcacg caaaactttt gaccatgtac
    15481 cagaacgatt tgcttcattt gcaaagaaat accagcaaga attgattttc gaagaagaaa
    15541 agaagcaagc tatttttaaa ctcagtcaac ttatgaaaga acatccagtc attacttttg
    15601 tttatggtgc aaaaaatgaa catcagaacc atgctgtttt attgaaaaaa tttatcgaaa
    15661 tggaggcatg caaatgaaaa tagggatcat tggtgccgga cctagaggat taagtatagc
    15721 agaaaggatt ctccataata atcaggacag acattctctt gaactttttc ttttcgatcc
    15781 caatggtcct ggaggtcgag tttggcggtt gaatcagcca ccggagttat taatgaattc
    15841 tgtttctcaa caagtgacgc tttttacaga tgaaacattg tccagtggag gattggtaac
    15901 acagggaccc aacttgtttc aatggagtaa aggacctgct acagattata tcgaacagac
    15961 ggtaaccaaa aataagcaac taattataga agaaatcaat cgtctggaaa aagatcagca
    16021 atctactcga tgtttatacg gactctatca acgctggttt ttctcaaagt tgcaagaaga
    16081 atttccagct tctattcagt ttgtttattc tttagtaaaa aaagtcagca aaagaaaaga
    16141 tggttatctt ttacagacag atcagcaaag ctattctatc gatcagcttg ttttagcaag
    16201 cggtcactgg gaaaatgatg agacagaaga agaaagagtg tgggtagact acgcaaagaa
    16261 aaatgattta ctgtatcagc ttccttctaa tcctgcagat gtcgatcttt cgaagatacc
    16321 gcctaaagaa aatgtctttt taagaggtct aggcttaagt ttttttgatt atgtcggatt
    16381 gtttacagta tcaagagaag ggcgcttcga gaacgaaaaa ggaaaacttg tctatcatcc
    16441 atcaggcaag gaaccgattg tatacagtgg atcaagaagg gggctccctt attaccctcg
    16501 aggaagaaac caaaagcaag gcggatcaat ggcatggcca cggttactaa caaaagaaaa
    16561 cctggagcaa tggcatcagt cgggattttt gactggcgaa acattttttg actatctgaa
    16621 aaaagacgtt gagttgtttt attacaagaa aatcatcgaa gaatatcatt tgccgattcc
    16681 aaagaaaaat tttgagcatg attttctctc cctctcttca gaggagtgtc tcggaaagca
    16741 tccagcacta atcccttata gatgggattg gtcgtattta gaaattcctc ttacctatca
    16801 ggatgagtcc tttgcagaag ctagccgagc atttattgaa gggcagataa aagaagcgga
    16861 aaaaggcaat tgcacaggag catttgcttc aacatttgat gcattaaaag actggcgtga
    16921 tccaatccgg caggctattg aatggcacgt tttttctgca aaggaataca aagaaatact
    16981 atggggatgg tttactcatc taaatagctt tttaacgatc ggtccgccag tcattcggac
    17041 gaaagaatta gcagcactga tagatgcggg tatttttcat ttgttcgaac cgccatttga
    17101 tattcagcaa aagcacaacc gctatgttat ttctctacaa gataaagaaa tcagcagtcg
    17161 ctatttgatt gaagctcgat tgccacaaac aagcttttct cgttcaagta atcctgtcat
    17221 aaaaaatttg aaggaaaaca aacttgttcg ccctttttca ttttcagata atacaggaag
    17281 ttatcattca gaagctatag atgttgagcg aggtacgaat cgcgtgattg atgcttcagg
    17341 ggaaatccag tctgactttt attgctatgg aatacctgtt gaaggtgtgg attggctaac
    17401 tgcagcagta gctagacctt atacagatcc atggaatctg agacaggcag ataaaatagc
    17461 acaacttata ttgactgaca aataaaaata cgcatgatga cttatctgtt ctttcacaag
    17521 tcttcatgcg tgtttttaaa taattattaa cgagaaacgc gtttgcgcat tgctttttta
    17581 cggttttctt cgatcattgc ttctttttcg tcaattggat cgatcactgt ttttttcaca
    17641 gaagcgacca aaccagcaac ggcagcggct gttgccagac ttcctactaa tattccagat
    17701 acaaattttt tcattgaatc cacctcattt attatttgtt atatctatta tggaacaaaa
    17761 gagaaaaaaa ggctagtttt acgtttgttt gtaaaccttt gcaaaaaatc cttgaaaatg
    17821 tttactaaat gttttattat aaatttacca tatgaattta cgaaatttta ctttgtttgt
    17881 tttcaccata aaagtaaact tcgttttttt aaattatata taatcaacaa acacaatttt
    17941 actaatcaaa ggagtgtttt ttatgacaat tttagtacta ggcggagccg gttatatcgg
    18001 ttcgcacgct gtagaccagc tagttcaaaa ggggtatcaa gtagctgtgg tagataattt
    18061 gctcacaggt cacaaacaag cagtacatcc agatgctcat ttttatgaag gagacatccg
    18121 ggataaagaa tttctacgtt ccgtatttga aaaggaaccg attgaaggag taatccattt
    18181 tgctgcaagc tctttagtag gagaatctgt cgaaaaacca ttgatgtatt tcaacaacaa
    18241 cgtatacggt atgcaaatct tacttgaagt catgcacgag ttcaatgtga ataaaatcgt
    18301 gttctcttct actgcagcaa cttacggaga accaaaagag tctccaatca cagaagatac
    18361 accagctaat cctaaaaatc cgtatggtga aagtaaattg atgatggaaa aaatgatgaa
    18421 gtggtgtgac caagcatatg gcatgcgcta tgtcgctttg cgctacttca atgtagccgg
    18481 agcaaaagca gatgcatcta tcggagaaga tcatacacca gaaacgcatc ttgtcccaat
    18541 cattttacaa gtagctcttg gtcaaagaaa agcattggct gtttatggag acgattatga
    18601 tacaccagat ggaacatgta tcagagatta tgttcaagta gaagatttga ttgctgcaca
    18661 tattttagct ttggaatact tgaaagaagg aaatgaaagc aacttcttca atttaggcag
    18721 cagcaaagga tactcagtca aggaaatgct agaagcagct agagaagtga caggaaaaga
    18781 aattccagca gaaatagcac caagaagagc tggagatcct agccgattag tcgcttcaag
    18841 tgaaaaagcg agagaggttc tgggttggaa acctgaatac acagatatca aagcaattat
    18901 caaaacagca tgggattggc atgtcagcca tccaaatggt tatgaagaat aggggaatga
    18961 gatatgtcaa tcagtcaaac gatttgtgat tttgccacac ttgcgatcga agctggcgga
    19021 tggatggaaa tggaccgctt atatttacag aatcgtcttt tagggatggt aggagaagat
    19081 gctctagaaa gcattgaagt aagaccagtt aaggaagaat ctgtagactt attggatcag
    19141 cttgtcgcta aggcaagaga aaatggtgtt ctttcagaat tgccagcaga acaagagatt
    19201 tttgaagcgc aattaatgga ttttctcaca ccgccacctt cagtcgtcaa tgcattcttt
    19261 gcacagcact attcgaaaag cccagaggaa gcaactgagt atttctttaa tttaagtaag
    19321 aaaaatgact acatcaaaac aagagccatc gcaaaaaaca tcaaatttcc ggcagagtcg
    19381 gagtatggtg aactggaaat cacaatcaat ttatctaagc cagaaaaaga tccaaaacaa
    19441 atcgcagcag aacgcgaggc ggtaaaagta gactatccta cctgcatgct ttgcatggaa
    19501 aacgaaggat atagaggtcg cttgaactat ccagcccgca ccaatcaccg gattattcga
    19561 atgaatctag acggtgaaag ctggggattc caatattctc cttatgctta ttacaatgaa
    19621 cattcgatca ttttatcaga acaacaccgc ccgatgaaaa tcacaaagga aacatttgct
    19681 agactgcttc ggattactga agtcttgcct cattactttg ttggttcgaa tgctgacttg
    19741 ccgattgttg gaggatcgat tctttctcat gatcattatc aggcaggacg tcatgaattt
    19801 cctatggcaa aagcaccgat agaaaaactt atcaaactag cagattatcc gcaagtcatc
    19861 gcaggagtcg tgaaatggcc gatgtctgtg atccgtttgc aatcaacaga gaaaaaattg
    19921 ttgattgaag cagctaactc agtattggaa aagtggaaag catattcgga tgaaacagta
    19981 tctatcagag ctttttcagc agatggtaca ccacaccata cgattacgcc aattgcacga
    20041 agaagggaca attactttga gttggacctt gtccttcgag acaacaatgt ttcagaagaa
    20101 catcctgatg ggatttttca tccacataaa gatgtccaac atatcaaaaa ggagaacatt
    20161 gggttgatcg aagtaatggg gcttgctgtt ttaccacctc gtttgaaacc agaactagta
    20221 gaagtagagc atttcttatt aggagaagaa aaccagatgg ctgaatatca tcgtccgtgg
    20281 gcagaacaat taaaaaaaga acatcctgat ttgacgaaag atacggtcac cgatgtcgta
    20341 caaaaatctg taggtcaggt ttttgcacgt gtactggaag acgctggagt attcaagcga
    20401 gatgaaaaag gacaggcagc gtttgaacga tttactgcaa ttttataaac gtaaaaaagg
    20461 aggagagaca aatggcaaca attaaagaca ttgcgcagtt ggcaggagtt tctcctgcta
    20521 ctgtttcaag agttctcaac tatgatcctg atctttccgt tggcttagcc acaaagcaaa
    20581 aaatttttga agcagcagaa cgcttaaatt atacgaaaca tcgtaaaaat gcagcaaata
    20641 ctaagcaaac gcttcgcctg atccagtggt acgacgatac ggaagagctt gaagatcttt
    20701 attatttatc tattcgtcta ggaatcgaaa aaaaggcaga agaagcaaac gttactttat
    20761 tgaaagaaac gtgggattcc attagtgatc ttccgacaga tggaacgatc gcactaggaa
    20821 aattcgacga agctcagtta ttagaaatga aaggatccca aaatgccttg cttttggtag
    20881 attctgacgg cacttctcaa ggaatcgact ctctagttgt cgactttaag tcaagtgttc
    20941 aaaaagtcat tgaacatttt gtctcccata aagcgcaaca tatcgctatg cttgctggaa
    21001 cagaatatac gaagaaaaac catcagcggc taaaagatcc cagaattatg gcatttcaac
    21061 aagttttggc agaaaaaggc tacgacgaag gtccaattat cgaagcagat ttttctgtcg
    21121 agtcaggata tcaagcagtg aagcagtatt taaaaacgaa tccgcatgtt cctgatgcgc
    21181 tttttgcagc taatgatgca ttagcgattg gcgcactgaa agcgattcaa gaaaaaggtt
    21241 tagtggtacc agaagatatt tctgtgatgg gtttcaatga tgtcagtgta gcgaaatatg
    21301 taacgccctc tctttctact gtaaaagttt ataccgaatg gatgggagag ctagcagtgg
    21361 agacgattct tcaattaagt aattccaaag caccggtttc aagaaagatt attgtggaaa
    21421 cagagttgat tattcgagaa tcaacgaaat aaaagatgga aaagctgtgg ggaaatgaag
    21481 tgataccgca gcttttttgt gtttcaggaa ggggcaaaat ttgcccgcca gcagataatc
    21541 atgtatcatt gtactagttt tgcctttggt attttcatcc tcagaataaa aaagtatgct
    21601 acaatggaag gcgagaatgt catagcagaa aggatcaaat atatgattac attaggaata
    21661 gatactgcaa accaaacttt ggcgatcggt gtggtagaag acgaacaaat tcttggacaa
    21721 atacaaacaa atatcaaacg caaccacagt gtaacactga tgcctgcaat cgaccaattg
    21781 tttgcagatc ttcaaataag tccaaaagac attgaccgaa tcgcggtttc tgatggtccc
    21841 ggttcttata cagggcttcg tataggtgta acgacagcaa aaacgattgc ttatacatta
    21901 gataaagagc tagtaggagt atctagtctg aaaaccatcg cggctaattg catcggtgtg
    21961 gataaaatta ttgtgcccat gtttgatgct cgaagaaaaa atgtatacgc tggggcatac
    22021 cgctggaaag aaaatagatt agaaattgta cttgaagatg tacatatttc tgcttctgat
    22081 ttgttcacac aactacaaaa gctgaatgaa gctatttatt tcgtcgggag tgattgtcac
    22141 aaattcttct atgaaataaa ggaaatattg cctagtgcga tgattaatac agttccagca
    22201 tgggatattc caagtggtgt aactgtggct caattaggtg ctttagctga accagttgca
    22261 gagaaacaag cttttcttcc tcgctattta aaacgggtag aagcagaaga aaaatggttg
    22321 gagacgcaca cacctggaga cgaaagttat gttgaaaaaa tttagagcgt atgccagaag
    22381 cttttttcaa gataaaatga tttatacaga aaaaacaatc gaacaaatcg atcgtcgtta
    22441 cgtactaaga gaattgacta cagaagatat caaagagctg ctggatgtcg aacgagaagt
    22501 ctataatggc gaattgccat ggacaaaaag tgcctttata tcagaattga agtcaccgct
    22561 tcctcatcta tatctaggta tctttgataa tggaagaatg attggtttta tcggttcaag
    22621 agcgttcggt ttagattgcc atatcacgaa tattgccgtt cgaccggcat atcagcgtaa
    22681 acgaatcggt tcgctgctta ttgacgaaat cgagaacttt gctataatga atcgctgtga
    22741 gacgatgtca ttagaagtgc gtatgagtaa tcaagatgca caaagacttt atcggaagtt
    22801 aggttttcaa gcaacaacga tcaaaaaaag atattacact gaaacaaaag aagatgcgtt
    22861 ggatatgata aaacttttag aaaatgagtg agattagttg atttgcagaa aaaaagattt
    22921 tgatgaagcg tacttggcag aatgcgtatg gatgattagc gaaaatgctt atccacatgg
    22981 ttcaccttgg acgaaaatgc agtttttggc agacatccaa cagcctcact ctgattatct
    23041 gcttttagtt gaagataacc agatctgtgg ctttattgga tactcgaaag taatagatga
    23101 agtcgagatc acaaatattg ctgttgcagt ttcagaacaa ggaaaagggc atgcacggca
    23161 tttgttacaa ttactgatta cggaacaaat aaaagattcg ctgcatgttt ttttagaagt
    23221 acgtctatct aatgaagcag caagaaaact ttatgaatca gaaaagtttc gtatattagg
    23281 taaacggaaa tcatattatc gcaatccgac ggaagatgcg atcattatga gcagaaagtt
    23341 aaaaacggag acgatgaaat gaatgaagaa ttgattctgg cgattgaatc aagttgtgat
    23401 gaaacgagtg tagcggtcgt tagaaatgga acagagatct tatctaacat cgttgcttct
    23461 caaataaaca gtcataaacg ttttggtggc gtagttcctg aagtagctag ccgccatcac
    23521 gtagaacaaa tcaccttatg cttggaagat gcactggtag aagctggtgt gtcggcagaa
    23581 gatttatcgg cagtcgctgt cacttatgga ccaggacttg tcggctcgtt gctgattggt
    23641 atttctgcag ctaaggcttt tgcttgggca caccaattgc cgcttattcc tgtaaaccat
    23701 atggcagggc atatctatgc agcccgtctg gttaaaccat tccagtttcc attgatggca
    23761 cttcttgtta gcggaggaca tacagaatta gtctatatgc aagaagatgg atcatatgaa
    23821 atcattgggg agacaagaga tgacgcagca ggagaagctt atgacaaagt tggaagagta
    23881 ttaggtcttt cttacccaag cggaaaagaa atcgatcagc tagctcatca gggaaaagat
    23941 aattaccatt ttccacgagc gatgatccat gaagataatt atgatttcag ttttagcgga
    24001 ttaaaaagtg catttatcaa tttggtgcat aatgcacaac agcgtggaga agatctagac
    24061 aaaaatgatt tagcagctag ctttcaagca agtgtgatcg atgtattgat tactaaaaca
    24121 cttcgagctt gtcaaaatta tccagtcaag caactgattg tagctggagg tgtagcggcc
    24181 aatcaagggt tgcgtgaagg attgcaggat gctttggcaa ctaaactccc tgaggtagaa
    24241 ctagtgattc cccctcttcg actttgtgga gataatgctg caatgattgg agcagcagca
    24301 catgtggaaa tgcaaaaaag aaattttgct agctatcagt tgaatgctga tccaagcttg
    24361 gttttatctg aataaagagg ttgtgaaaaa tctgccctgc atcaagtaat aagaacagcc
    24421 caggaaaaac ggcttctcat gtttttgacc tggctgttct tattaccgcg tatgcaagct
    24481 tttgaacgcc gtttaatcaa gagttcgtaa gcctgtcgtt taactctgat tcacaaggaa
    24541 caatttcaaa aaacagctcc tcatgtttat gaacattatg acttgtgacc gaagagttga
    24601 caggtacaca ccgtttaatc gaaataaggg ctgtgacacg cttttgtcaa agccctattt
    24661 tttacagctt attcatcatt atccgttcct ctgattgccc tcactgatta atcgtttatt
    24721 gtcgaggaat atcgaataat ctaggtcgga tgaggacaag acgcttcctc tagttaatct
    24781 atcagtattt tttgcaaaca gtgaatatta cataactgaa acacacatag aaaatctttg
    24841 agtcttgccc agtgatctaa aactgccgga tctttcagaa gttttaggga ggaaaataag
    24901 cagtgaccct gataaa
//
LOCUS       DAZUMO010000034        24576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000034 DAZUMO010000000
VERSION     DAZUMO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.034"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..552)
                     /locus_tag="ACG8ER_001506"
     CDS             complement(154..552)
                     /locus_tag="ACG8ER_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS5050094.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="ACG8ER_001507"
     CDS             complement(549..1322)
                     /locus_tag="ACG8ER_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5050095.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="ACG8ER_001508"
     CDS             complement(1319..2002)
                     /locus_tag="ACG8ER_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5050096.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="ACG8ER_001509"
     CDS             complement(2169..3056)
                     /locus_tag="ACG8ER_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5050097.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="ACG8ER_001510"
     CDS             complement(3053..3922)
                     /locus_tag="ACG8ER_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5050098.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="ACG8ER_001511"
     CDS             complement(4059..5345)
                     /locus_tag="ACG8ER_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5050099.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="ACG8ER_001512"
     CDS             5618..6196
                     /locus_tag="ACG8ER_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5050100.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="ACG8ER_001513"
     CDS             6196..7125
                     /locus_tag="ACG8ER_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050101.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="ACG8ER_001514"
     CDS             7122..8858
                     /locus_tag="ACG8ER_001514"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5050102.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="ACG8ER_001515"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="ACG8ER_001515"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS5050103.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="ACG8ER_001516"
     CDS             complement(9754..10263)
                     /locus_tag="ACG8ER_001516"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS5050104.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="ACG8ER_001517"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="ACG8ER_001517"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS5050105.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="ACG8ER_001518"
     CDS             complement(11564..12655)
                     /locus_tag="ACG8ER_001518"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS5050106.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="ACG8ER_001519"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="ACG8ER_001519"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS5050107.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="ACG8ER_001520"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="ACG8ER_001520"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS5050108.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="ACG8ER_001521"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="ACG8ER_001521"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS5050109.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="ACG8ER_001522"
     CDS             complement(16618..17592)
                     /locus_tag="ACG8ER_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS5050110.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="ACG8ER_001523"
     CDS             complement(17573..19456)
                     /locus_tag="ACG8ER_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS5050111.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="ACG8ER_001524"
     CDS             complement(19474..21699)
                     /locus_tag="ACG8ER_001524"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5050112.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="ACG8ER_001525"
     CDS             21814..22848
                     /locus_tag="ACG8ER_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5050113.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="ACG8ER_001526"
     CDS             complement(22915..23928)
                     /locus_tag="ACG8ER_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5050114.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="ACG8ER_001527"
     CDS             complement(24006..24194)
                     /locus_tag="ACG8ER_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS5050115.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7062 a         5340 c         4397 g         7777 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaaat
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaact
//
LOCUS       DAZUMO010000035        24318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000035 DAZUMO010000000
VERSION     DAZUMO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.035"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..606
                     /locus_tag="ACG8ER_001528"
     CDS             289..606
                     /locus_tag="ACG8ER_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5050116.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="ACG8ER_001529"
     CDS             complement(697..1062)
                     /locus_tag="ACG8ER_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050117.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="ACG8ER_001530"
     CDS             1270..1752
                     /locus_tag="ACG8ER_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050118.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="ACG8ER_001531"
     CDS             1949..2839
                     /locus_tag="ACG8ER_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS5050119.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="ACG8ER_001532"
     CDS             3165..5639
                     /locus_tag="ACG8ER_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5050120.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="ACG8ER_001533"
     CDS             complement(5742..7478)
                     /locus_tag="ACG8ER_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5050121.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="ACG8ER_001534"
     CDS             complement(7475..8179)
                     /locus_tag="ACG8ER_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5050122.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8815
                     /locus_tag="ACG8ER_001535"
                     /pseudo
     CDS             8310..8815
                     /locus_tag="ACG8ER_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
     gene            8775..9113
                     /locus_tag="ACG8ER_001536"
     CDS             8775..9113
                     /locus_tag="ACG8ER_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS5050123.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9134..10552
                     /gene="ffh"
                     /locus_tag="ACG8ER_001537"
     CDS             9134..10552
                     /gene="ffh"
                     /locus_tag="ACG8ER_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS5050124.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDTVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10664..11242
                     /locus_tag="ACG8ER_001538"
     CDS             10664..11242
                     /locus_tag="ACG8ER_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS5050125.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11246..11767
                     /locus_tag="ACG8ER_001539"
     CDS             11246..11767
                     /locus_tag="ACG8ER_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS5050126.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11846..12400)
                     /locus_tag="ACG8ER_001540"
     CDS             complement(11846..12400)
                     /locus_tag="ACG8ER_001540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5050127.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12653..12928
                     /gene="rpsP"
                     /locus_tag="ACG8ER_001541"
     CDS             12653..12928
                     /gene="rpsP"
                     /locus_tag="ACG8ER_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS5050128.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12940..13191
                     /locus_tag="ACG8ER_001542"
     CDS             12940..13191
                     /locus_tag="ACG8ER_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS5050129.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13315..14106
                     /locus_tag="ACG8ER_001543"
     CDS             13315..14106
                     /locus_tag="ACG8ER_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050130.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14276..14797
                     /gene="rimM"
                     /locus_tag="ACG8ER_001544"
     CDS             14276..14797
                     /gene="rimM"
                     /locus_tag="ACG8ER_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS5050131.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14787..15548
                     /gene="trmD"
                     /locus_tag="ACG8ER_001545"
     CDS             14787..15548
                     /gene="trmD"
                     /locus_tag="ACG8ER_001545"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS5050132.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15684..16031
                     /gene="rplS"
                     /locus_tag="ACG8ER_001546"
     CDS             15684..16031
                     /gene="rplS"
                     /locus_tag="ACG8ER_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS5050133.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16341..16583
                     /locus_tag="ACG8ER_001547"
     CDS             16341..16583
                     /locus_tag="ACG8ER_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS5050134.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16671..16817)
                     /locus_tag="ACG8ER_001548"
                     /pseudo
     CDS             complement(16671..16817)
                     /locus_tag="ACG8ER_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16874..17188
                     /locus_tag="ACG8ER_001549"
                     /pseudo
     CDS             16874..17188
                     /locus_tag="ACG8ER_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            17217..18476
                     /locus_tag="ACG8ER_001550"
     CDS             17217..18476
                     /locus_tag="ACG8ER_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5050135.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18529..19425
                     /locus_tag="ACG8ER_001551"
     CDS             18529..19425
                     /locus_tag="ACG8ER_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5050136.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19436..20305
                     /locus_tag="ACG8ER_001552"
     CDS             19436..20305
                     /locus_tag="ACG8ER_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5050137.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20315..20990
                     /locus_tag="ACG8ER_001553"
                     /pseudo
     CDS             20315..20990
                     /locus_tag="ACG8ER_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21196..22860
                     /gene="alsS"
                     /locus_tag="ACG8ER_001554"
     CDS             21196..22860
                     /gene="alsS"
                     /locus_tag="ACG8ER_001554"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS5050138.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22873..23583
                     /gene="budA"
                     /locus_tag="ACG8ER_001555"
     CDS             22873..23583
                     /gene="budA"
                     /locus_tag="ACG8ER_001555"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS5050139.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23713..24210
                     /locus_tag="ACG8ER_001556"
     CDS             23713..24210
                     /locus_tag="ACG8ER_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS5050140.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         8053 a         4150 c         5017 g         7098 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtgtatg
     8521 gacgacaagc ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca aagcctatca
     8581 aaaattgcag gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc cttcatgcgg
     8641 aacccattcg atttatgacg ggagtttttc cggaataaaa aaaaatggtg ccggagtggc
     8701 tactgcttat tttattcagc aaaaaatgac tgtgctctct gatgaagaat ggctgaaaac
     8761 tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc tttgaatttt
     8821 attcgacctt attgacagaa aaacaaatga actacatgga attatattat gcagatgatt
     8881 tttcattagg tgagatcgcg gaagaatacg aagtaagccg acaagcagta tatgacaata
     8941 tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa acttcatctt ttttctaatt
     9001 atattgttcg agaacaggta ttggaaaata tgactgcata tattacagat aagtacccgg
     9061 aagataaaaa actacttgaa tatgtacagc aaattcaagc aatcgaagaa taaataaaag
     9121 gaatgaagaa aatatggcat ttgaaagctt aactgaacgt ctccagcagg cgatgggaaa
     9181 aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc gggaaatccg
     9241 ccttgcctta ctagaagccg atgttaactt gcaagtagtc aaagatttta ctaagcgcgt
     9301 acgcgaacgt gcggtagggg cagaagtgct ggaaagttta tcacctgcac aacaaatcgt
     9361 aaaaatcgtt gatgaagaat tgacgatcac acttggttct gaaacagcag agttaaataa
     9421 atcaccaaaa ataccaactg ttatcatgat ggctggtctt caaggggcag gtaaaacgac
     9481 ctttactggt aaattagcca attatttaaa gaaaaatgaa aatgctcgtc cgctcctaat
     9541 cgcaggggac gtatatcgac cagctgcgat cgaccaattg aaagtattag gtcaacagct
     9601 agacgttcct gtatttgata tgggaactga agtcagcccg gtagagatcg tccgtcaagg
     9661 gatggagtta gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg caggacgtct
     9721 gcatatcgat gaaacattga tggatgagtt gaaacaaatc aaagaattga cgcaaccaaa
     9781 tgagattttg cttgttgtcg atgccatgac tggtcaagat accgtcaacg tagctgatag
     9841 cttcaaccag caattaggta ttacaggggt cgtcatcact aaacttgatg gggatactcg
     9901 aggcggggca gcactatcga tccgctcagt gacaggcgca ccaatcaaat tcatcggttc
     9961 tggtgaaaaa ttgactgact tagaagtatt ccacccagat cgtatggcta gccgtatcct
    10021 tggtatgggg gatatgctga cattgattga aaaagcgcag caagactatg acgagaaaaa
    10081 agcggaagaa cttgcacaga aaatgcggga aaattcattc gatttcaatg actttattga
    10141 acagcttgat caagtgatgg gtatgggccc actggaagat ctaatcaaaa tgatcccagg
    10201 aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg ttgaacgcaa
    10261 aaaagcgatg gtttattcta tgacgccagc agaaagagca aatcctgatc tgttgaaccc
    10321 aagccgtcgt cgccgaattg ctgccggttc tggaaacagt gttgttgaag tgaatcgaat
    10381 gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag gggatatgaa
    10441 catcccaggt atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag gcaaaatggc
    10501 gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat aagatgaaaa
    10561 aaacagcttc ggtacagact gaaaatgtct actgccggag cttttctatc agcaattttt
    10621 attctttact aatacaatta ctctaaacga agaggtggca cagatgagat gtccgtggag
    10681 tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc tggggtgttc ctgaatatga
    10741 tgaccaaaag ctgttccgaa aattgatgct tgatattaat caagccggat tgagctggca
    10801 gacgatttta aataaaagtg aagcattcga tgaagcatat gatcgattcg atattaaaaa
    10861 agtcgcaaat tatggagaaa caaaaatcag tgaactaatg gaaaacaaag gaattattcg
    10921 taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga aaattcaaga
    10981 agaaatcggc agtttttcag agtatctttg gtcgttttct aatggaaaaa tcgttgattc
    11041 ttcttatcat tcacaagaag aagtcccaac aacaagtcct ctttcagatg aattagcaaa
    11101 agatctaaag aaaagaggat ttaaatttat cggaagcaca acaatctatg cttttttaga
    11161 agctgtcggg atcatcaatg atcaccttga cagttgtttc cgcaaagaag aggtcgtacg
    11221 tatcggcaga aagggcgaat aaagcatgcg aaaaatcata ttttatggtg ctataagttt
    11281 agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca ctccaacagg
    11341 taagcaaacc acttatgaag ctttttatga aacaatcgat acgaccatca tgggcagaaa
    11401 aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag agaaaaagaa
    11461 tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg taaacgaaga
    11521 tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct gggttgtggg
    11581 tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat ggtatatcca
    11641 gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa catatgaagc
    11701 gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc attatgtacg
    11761 aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct gtacatagtt
    11821 gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa tctagatacg
    11881 catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca ttttcatcaa
    11941 ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc acattcaaaa
    12001 agacaagtga ttgtcgtaga tggtggtttg cgccaaagcc gccgatccca cctggtgatg
    12061 cagtaacgac taaacctggt ttatgatccc aaacgctgtg tccataaggt cttgagccta
    12121 catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga gtaacgaaaa
    12181 cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca ggagcctgtt
    12241 ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca aatacacttt
    12301 cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg taagaatctt
    12361 ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc ctccttcaaa
    12421 ctatctataa caatcttaca aaaaataagt aactggagca aataaaaaca cttactgaca
    12481 aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa aatcttctat
    12541 ttcctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac agggtaaaaa
    12601 aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa taatggcagt
    12661 taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta tcgtcgtagc
    12721 tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt acaatccttt
    12781 gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt tatcaaaagg
    12841 tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta tgcaaaaaca
    12901 ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt aagcgactta
    12961 gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt agacgtcgta
    13021 gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat tggccgtatc
    13081 attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg tgttcggatc
    13141 aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta aataaacgtg
    13201 ctgtactcga attttcgagt gcagcttttt tgttttttta ttaattgaag aaatgattat
    13261 ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca gccaatgaga
    13321 aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc ggttactcaa
    13381 gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag tccagaaaaa
    13441 agtagtacca aaacggctac caccactgaa caaacaatgg ctacctcaaa taatgagaaa
    13501 aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc tctagatgta
    13561 ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg ttattctatt
    13621 ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg
    13681 cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac aatccaagtt
    13741 ttgcagccct ttgagatcga cacaaatggt cagcaagtgg acttaggttc tcgaatcaca
    13801 gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg aaactggcgt
    13861 attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc taaagaaatc
    13921 gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa aattactgtt
    13981 gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc
    14041 tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc tatgaagagg
    14101 ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca ggaagtaatc
    14161 aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag aaaactagaa
    14221 ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat ataagttgac
    14281 tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt
    14341 tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgt taacactatt
    14401 ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac ataaaaattt
    14461 tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat atcgggatgg
    14521 catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat tctattacca
    14581 tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag taaaagaaat
    14641 cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt
    14701 gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag tggtccgtgt
    14761 agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta tttccaagaa
    14821 tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa ggattattag
    14881 atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca gtagatgatt
    14941 atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat gataacatcc
    15001 aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt gacccagctg
    15061 gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac ttagtattca
    15121 tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca gatgaagttt
    15181 ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg atcgatgcaa
    15241 cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg gattcttatt
    15301 caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa gggatgaaag
    15361 taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa ttaaaagaat
    15421 cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa tggacacctc
    15481 aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag
    15541 ccaagtgact ttttctaaaa tattctttac agtaggttga taatatgtta gtattttatg
    15601 gtgagtgcaa aagcacttaa ctattacgat attccgctat gagaaaagct cataagaata
    15661 tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca aaagaacaat
    15721 tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg
    15781 ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg
    15841 ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc gttgaacgta
    15901 cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac
    15961 gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa
    16021 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
    16081 ttcttagcat gtaatttgaa caccctgatc cagttttttt ctttaattat tggtagggga
    16141 gaataatgga ttagaataaa tctacctata agtagtagta aaaaaaagag cactatgata
    16201 atatctttct taagagtaac cgctttaata aaaaatccag aatattctaa cataaatgga
    16261 gtattcttag aatcaaaagg agaaagaaga tggtttatac ttatacggag caagaaaaaa
    16321 tgaaagtttg agacacattc atgaatgatg gaaaagtaaa acaagtacca agttcaacaa
    16381 aaaagaaaaa catcctattg aaagaagtct tgaaacgctt tgaccatgga gtgacttata
    16441 cagaaacaga ggttaattcg atactgctga acgtattttc tagtggcgat tatgtcgaac
    16501 aaagacgtta tctaatcaca tttggctttt tcaaaagaag ttcagacggt cgggcatatc
    16561 agatgatggg aattgaaaat tagaagtttt aatttatact cattgaaaaa agttcaagta
    16621 tgcgatattg gttctacacc gcatacttga actttttttg tcatctgtat ctataataca
    16681 aagccaagaa cgttattcaa aatatggata gctatgataa aagctaatga gcgattcgca
    16741 gttaaataag caatcgtcag taaaatcgtt ccaggagcgt acatgaaaaa atcaattgga
    16801 tgcgttacat gaataaagat aattctcttt tatttgtacg aataaatacc aaaacgtacc
    16861 tcctaaaatt agattcaaag aagatagcaa agaggaaaaa gatagttctg cagcagctat
    16921 agcggcatgt ggattgctag aactttccaa acaactgcct gtttcagatc ctcgtcattt
    16981 cgattacgaa gagatagcaa tcaaaatctt ggaagaatta cagaaaaact atacaacgaa
    17041 acgagttaac ggatcaaacg gtctgcttct ccatgccgtt tatgataaaa acagcttaaa
    17101 aggtgtagat gaatgtgtga tctggggcga ttacttttat gtagaaggaa tcactcgtct
    17161 agctaaaaca tggtactgtt actggtgatg tataagagaa gaggaggatt catataatga
    17221 agcgatggaa aaaaatgatg attggatcaa tagttttagg aatgtcactc gtatttggtg
    17281 cttgtggtaa tcaagaagac agtagtgaag aatcagcaaa tgaaattacc gtttcgacat
    17341 ggaattatga aacaacacct gaattcaaag cgctctttga agcattcgaa aagaaaacgg
    17401 gaatcaaagt gaaagcagta gatattgctt cagatgatta tgatacgaaa ctcaccacga
    17461 tgctttcttc tggagacacg acagatgtat tgacgatgaa aaatcttttg tcgtattcaa
    17521 attatgcttt acgagatcag ttagtcgatc aaacagaacg aattagagaa ttagacactg
    17581 atgctgctga agaaacatat gaaatgtatg atatcgatgg aaaaacatat gcattgccat
    17641 atcgaacgga cttttgggta ctatattaca ataagaaaat gtttgatgat gcaggaatcg
    17701 aatatccaaa aaatctaact tgggacgaat atgaagattt agcaaaaaaa cttagtaaaa
    17761 atgacggaca agtttacgga gcatatcaac atatctggcg ttcgacaatc caagctattg
    17821 cagctgctca aaacgaggcg aatcttgttg aaccagatta tcaatatatg actgattatt
    17881 atgatcgagc attgaggatg caaaaagaag gcgcgcagat ggattttggc acggctaaat
    17941 caacgaaagt aacctatcag tctcaatttg aagaacaaaa agcagcaatg atgtacatgg
    18001 gaacttggta tatggctggc atactcgcaa acaaagaagc aagtaagaca gaagtagaat
    18061 ggggaattac agctattcct cagaagaaaa aaggagaatc ggtcacgacg tttggctcac
    18121 caacagcatt tgctgtcaat aaaaactcaa agaaacaaaa agcggcgcaa gaatttattg
    18181 aatttgcagc cagtgaagaa ggagcaaaag tattagcagg agtaggagta gttccttcat
    18241 atcgtacgga tgagattgat cagctttatt ttaatctcgc tggaatgcca acggatgaaa
    18301 tttctaaaaa agctttttct cctgatgaaa tcaaacttga atttccgatt gatacgtatg
    18361 ggcctgcaat cgataagatt ttacaagaag aatatgactt agttttggtt ggagatgaaa
    18421 cgccggaaaa aggaacggta aatatggaga aacgtgtagc tgcggaaaaa gaataatcat
    18481 gaaaggagag aagccaagag ataaaacact cttggtttcg tcatcgaaat ggagaatagt
    18541 ataaataaga aagctacaaa tcatttgcgt agaaaacgaa cgtggatcgc ttattcgttt
    18601 atcgccccga atttcattgg ttttttcgtt tttacattaa taccaatcct gttttcgctt
    18661 gtactagcat ttatgaaatg ggattctttt tcaacgccag aatttgctgg actagataac
    18721 tttcgtaaaa tggccaacga tgagacattt tggatttctt tgaaaaatac ttttttatat
    18781 accgctgctg tagtgcctgg cacacttgtt tgttcgttag ggctagcttt actactaaat
    18841 cagaaaatta gagggatgaa gctttttcgt acgatatttt tctttccgta tgttacttcc
    18901 ttagtagcaa ttgctgttgt ttggaatatg ttgtttcatc caacagtggg acctattaat
    18961 atctttttaa gacgatttat tgaacatcct ccaggttgga cttcaagttc aaattgggcg
    19021 ctttgggcaa ttgtaattgt cagtatttgg cgagggatgg gttattacat gattctatac
    19081 cttgcaggac tacaaagtat ccaaaaagaa ctatatgaag cgtcttcgct agatggagca
    19141 aaccgttgga aacaattttt gaatgtgaca ttaccttcgt tgagaccaac aaccttcttt
    19201 gtaacgatca tgcttgtcat caattgtttc aaggtgtttg accttgtgca agtgatgacg
    19261 gatggcggcc ctggtcgagc gacaaatgtg ctggtttacc aaatctacaa tgaagcattc
    19321 aaaaaatttg attttggtta tgcttcagct atttcacttg ttttgttcgt aattgttttg
    19381 ggcgttacgg ttgttcaatt ttcttggaat aagcgtcgta cttaggaggt gtgatatgga
    19441 agagagtgtt cgaaatgaaa gaaaaaaagc aaaaattcgt tcaaaaaaca tgccctttat
    19501 tcaatggttg ggtatgattc tgctgattgt attagcttta ttgacgatca ttcctttttt
    19561 atggatggtt tctgcttcat tcaaagcaaa taatgaagtg ttaacgattc cgattcaatg
    19621 gatccctaaa acttggcatt tagagaatta tctggttatt tggtctcgta ttcctttagt
    19681 gacatttttt aagaatacag cttttttaag cgttgtgatc acgttgattc agttgtttac
    19741 ttctagtttt gctgtttacg ggttttctga actaaatttt aaagggcgcg atattttatt
    19801 tttagcatat atcggcacga ttgctgtccc ttggcagtct tatatgatcc cgcagttcat
    19861 tatgatgaga aaagtgggtt taacagatac gttgtggtcg ttggtgctat tacaagcgtt
    19921 caatgctttt ggtgtgttct tgttgaagca atattacagt agtatcccag attctttatg
    19981 tgagagtgcg cggatcgatg gcttatcaga gtgggaaatc tatcgcaaga tcattttacc
    20041 tcttacaaaa ccggcgattg ccagtttgac gatcatcaca ttcgttaata catggaacga
    20101 ttatatgggg ccattcattt atttgtcttc tacggaaaat aaaacaatcc agttagggtt
    20161 gaaaatgttt attgggctat ttgatgcaga gtacgcatta atcatggctg cctcagttgt
    20221 ttctgttctt cctgttgctt ttatattttt agcgatgcaa aaatattttg tagaagggat
    20281 cgcttcatca ggaatgaagg aataggaggt gattatgaaa gagcagcgtt ttgacaaaaa
    20341 tgtttatatg gaattctttt attggttgta tcaatcattg gcaagtagcc tttgtttttg
    20401 gtgtgtcaat atcttgtata tagcagctgc gttccttttg tcgatagatg gaaggaatgt
    20461 cttttccttt attttttctt tactttttat tggtccaggt tgcatcgtga tgataggtat
    20521 gacagaagaa ttagcaaaag aaaaaagttt ttcaccagta cgtttgttca taaagacgtt
    20581 tcgccaattt tggctaaagg gattttttta ttggctattt gcttggatag ttagtgtgat
    20641 aatgattttt gattgtttct tttttatacg tttttcttat ggaaaatggt tgattccgtt
    20701 gtttgtttta cttgcttgtt ttacttgctt gtttatcagt ttcctttagc atcaactgct
    20761 ggtattttca ggtgagaaat ccagcgtcga aaccaaatca agttctacgg atagcgttct
    20821 actatacgtt gaaaaaatgg tatgtgagtt tactagattt tttactattg acaagtttgt
    20881 ttctcttctt tttcgtaaag ccccaatggt gtattctact aggtccttct atcgtatttg
    20941 gtttgattta cttcaacaat cgtaaattga tgagaactat ggatctgtag actttataaa
    21001 ttgaaacttt caattaagaa tgacagtttt ttatgtaaaa actgtcattc tttttcattt
    21061 tttgaaacat atggctaaac aaatgaaata aaacatgcta tacttttgaa tgaaacaggt
    21121 ttagaaactg tatcacttat ttgtgattat ttctaactaa taattaaaaa taaagcataa
    21181 aaaggacgat gttcaatggc tgaaaaaatg aaacaaggtt cgaaatctgt agtagaaagt
    21241 ttgattaatc atcatgttga ttatgtattc ggtataccag gtgcaaaaat cgatggtgta
    21301 tttaatgaat tagaggatca aggtcctgaa ttaatcgtga ctcgtcacga acaaaatgct
    21361 gcatttatgg cacaagcaat cggacgtatt acaggagagc caggagtagt tatcgcaaca
    21421 agtggtcctg gtgctagtaa cttagctaca ggtttggtaa cagcgacagc agaaggtgac
    21481 ccagttttag caattgccgg acaagtaaaa cgaagcgatc tattgaagtt gacccatcaa
    21541 agcatggata atgcagcact gttccagcca atcacaaaat acagtgcaga aatccaagat
    21601 ccagaaacaa tttcagaagt gattgccaat gcttatcgta tggctaagag ttcaaaaaaa
    21661 ggcgcgagct ttatcagtat cccgcaagat gtagtggatg cacctgtcca aggtaatgtc
    21721 atcaagccat tgagcgatcc taaattaggt tctgcttctg cagatgatat ccgttaccta
    21781 gctgaacgca ttagagaagc aaaactaccg gtactattag taggtatgcg aggatcaagt
    21841 gaaaaagaaa cgttagccat caggcaatta gttgaaaaga cagcattgcc tgttgtagag
    21901 acattccaag cagctggagt gatctcacgt gaattagaag cgcatttctt tggtcgtgtg
    21961 ggtttattta gaaaccaacc aggagatatg cttttgaaac gaagtgatct agttattgca
    22021 attggctatg atccaatcga atatgaagca agaaactgga atgcggaaaa agatgcgcgg
    22081 atcattgtga tcgatgaagc gccagctgaa atcgatcctt ttatgcagcc tgaaagagaa
    22141 ttgatcggtg atatctcagc cactcttgat ttattgacag gttcattaga gcctcaacaa
    22201 gtgtcagaag atgcaaaaga atatttagca tctcttcaag caaaattaac ggagcgggac
    22261 attgttcaat caaaaggaga agcaggtatc ttgcatcctt tagaagttat taacacgctt
    22321 cagtcaaaag taacagatga tatgactgtt accgtagatg taggtagtca ttatatttgg
    22381 atggcacgcc actttagaag ttatgaacct cgtcatttgt tattcagtaa tggtatgcaa
    22441 acgcttggtg ttgcattgcc atgggcgatt tctgctgcat tggtgcgtcc aaatacacag
    22501 atcgtatcag tttccggcga tgggggattt ttattctctg cccaggattt agaaacggcg
    22561 gtacgtaaaa aattgaatat cgttcatttg atttggaatg atggacacta caatatggta
    22621 gagttccaag aaaaaatgaa atatcagcgg gcgtcaggtg tagactttgg tccagttgat
    22681 tttgtgaaat atgcagaagc ttttggcgct aaaggaatcc gagctacaag tgtagaggaa
    22741 ttagaaaaag ctttagaaga aggatttgcg acagaaggac ccgtaattat tgatattccg
    22801 attgattacc gtgataatga gaaattggga gaaacaatct taccagatca attctattaa
    22861 ggaagtgtcg acatgactga aaaaatctta taccagcacg ggacattagg agctttgatg
    22921 gccggcttga tggatggaac agaaacgatt gctcatatat tagaaaaagg aactctggga
    22981 ttgggaacat tgcatgggct tgatggtgaa gtcatctttt tagatggcgt agcttatcaa
    23041 ggaagatcag atggctcagt tgcccaatta gatggaagtg aacttacccc ctatgcagca
    23101 attacagatt ttacgccaga tactagtttt tctgtctcaa aaactgcaga tggagaacaa
    23161 ttgaaaaaag acatattagt aagagaagcc ggggaaaacc tatttttggc agtgaagata
    23221 actggattat tcaaaaatat gcacatccgt atcatgccga agcaagaaaa accttacaga
    23281 agactagcaa aaatttcaga gtctcaacca gaattccaac aaagcaatgt ccaaggaaca
    23341 ttagtcgggt tctttacgcc tgaattattc caaggagttg ctgcagcggg atttcacctg
    23401 cattttatcg atgatacaca tacatttggc ggacatgtaa tggattttga agtggctgaa
    23461 ggaaaagttg agattagccg catctcgtcc cttgtacagc attttccagt tgatgatccg
    23521 acttttgttc acgaaacgat tgattatgct gatttagcag cagaaatcca acaagcagaa
    23581 tagaaaaaga cagcgagatg cgttcaaggt gattaacctg acgtatctcg tttttttgag
    23641 gagaaaacct agcgtttaca aaaaaactga gctataatct tctccataga tcaaaaagga
    23701 gcggttttta tcatgtggac agaaaaacag aaaaaagatg catacgaaat tttattatta
    23761 cagcaacaag gattattaga agcagaagat aactggttag caaatttagc aaattcatct
    23821 gcattgttga atgagacttt gcctgagact gtctttgcag gatattattt attcgatggt
    23881 aatgaattga ttttaggacc gttccaaggg cgagtttctt gcacacgtat caagatggga
    23941 aaaggagttt gtggagaatc agctgaaaaa caagaaacat tgatcgttga tgatgtcaaa
    24001 acccatgcaa attacatttc ctgcgattca gccgcacaat cagaaatcgt agtgccaatg
    24061 gtaaaagaag gtaatttaat cggtgtttta gatatcgatt gcggagtaac gaaagggtat
    24121 gatgaaatcg atcaagcatt tctagaaaaa tatgtagatc ttttattagc aaattttaat
    24181 ataaatgctt tttcatttat taataactaa aatcgtctat tgaaacaaaa aagaagagaa
    24241 atcaagaata aaaagagccg gaaagacagt gggcatttct gtttttcggt ttttttattt
    24301 ttttataaaa tcacaagt
//
LOCUS       DAZUMO010000036        23643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000036 DAZUMO010000000
VERSION     DAZUMO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.036"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            270..677
                     /locus_tag="ACG8ER_001557"
     CDS             270..677
                     /locus_tag="ACG8ER_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050141.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            691..1092
                     /locus_tag="ACG8ER_001558"
     CDS             691..1092
                     /locus_tag="ACG8ER_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050142.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1094..1465
                     /locus_tag="ACG8ER_001559"
     CDS             1094..1465
                     /locus_tag="ACG8ER_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050143.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1501..1803)
                     /locus_tag="ACG8ER_001560"
     CDS             complement(1501..1803)
                     /locus_tag="ACG8ER_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5050144.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2061..2174)
                     /locus_tag="ACG8ER_001561"
                     /pseudo
     CDS             complement(2061..2174)
                     /locus_tag="ACG8ER_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2557..3789)
                     /locus_tag="ACG8ER_001562"
                     /pseudo
     CDS             complement(2557..3789)
                     /locus_tag="ACG8ER_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            4043..4612
                     /locus_tag="ACG8ER_001563"
     CDS             4043..4612
                     /locus_tag="ACG8ER_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5050145.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4790..5230)
                     /locus_tag="ACG8ER_001564"
     CDS             complement(4790..5230)
                     /locus_tag="ACG8ER_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS5050146.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5388..6152
                     /gene="map"
                     /locus_tag="ACG8ER_001565"
     CDS             5388..6152
                     /gene="map"
                     /locus_tag="ACG8ER_001565"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5050147.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6184..7107
                     /locus_tag="ACG8ER_001566"
     CDS             6184..7107
                     /locus_tag="ACG8ER_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5050148.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7183..8322
                     /locus_tag="ACG8ER_001567"
     CDS             7183..8322
                     /locus_tag="ACG8ER_001567"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS5050149.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8315..9115
                     /locus_tag="ACG8ER_001568"
     CDS             8315..9115
                     /locus_tag="ACG8ER_001568"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5050150.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9115..9942
                     /locus_tag="ACG8ER_001569"
     CDS             9115..9942
                     /locus_tag="ACG8ER_001569"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5050151.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9947..10654
                     /locus_tag="ACG8ER_001570"
     CDS             9947..10654
                     /locus_tag="ACG8ER_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5050152.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            10754..11620
                     /gene="rfbA"
                     /locus_tag="ACG8ER_001571"
     CDS             10754..11620
                     /gene="rfbA"
                     /locus_tag="ACG8ER_001571"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS5050153.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11634..12206
                     /gene="rfbC"
                     /locus_tag="ACG8ER_001572"
     CDS             11634..12206
                     /gene="rfbC"
                     /locus_tag="ACG8ER_001572"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS5050154.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12228..13256
                     /gene="rfbB"
                     /locus_tag="ACG8ER_001573"
     CDS             12228..13256
                     /gene="rfbB"
                     /locus_tag="ACG8ER_001573"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS5050155.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13354..14205
                     /gene="rfbD"
                     /locus_tag="ACG8ER_001574"
     CDS             13354..14205
                     /gene="rfbD"
                     /locus_tag="ACG8ER_001574"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS5050156.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14240..16273
                     /locus_tag="ACG8ER_001575"
     CDS             14240..16273
                     /locus_tag="ACG8ER_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050157.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16317..17597
                     /locus_tag="ACG8ER_001576"
     CDS             16317..17597
                     /locus_tag="ACG8ER_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS5050158.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17807..18613
                     /locus_tag="ACG8ER_001577"
     CDS             17807..18613
                     /locus_tag="ACG8ER_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050159.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18625..19845
                     /locus_tag="ACG8ER_001578"
     CDS             18625..19845
                     /locus_tag="ACG8ER_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050160.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19835..21421
                     /locus_tag="ACG8ER_001579"
     CDS             19835..21421
                     /locus_tag="ACG8ER_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5050161.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21460..23598
                     /locus_tag="ACG8ER_001580"
     CDS             21460..23598
                     /locus_tag="ACG8ER_001580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5050162.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         7558 a         3937 c         4848 g         7300 t
ORIGIN      
        1 agttgttgat atagttgcta atattgttag ctaatttgtt agtaaaactg tatacgaatt
       61 tagatagtaa aaaaacagtt tgttccttat atagaggcac ttttactact gattaaatgt
      121 cagatatgct tacaaatata cttataaata tgcttacaaa attagctaat atttccgttg
      181 acaaagcttt gttactattg ttaaactaaa ttgactagca aaagtgaaac ataaatttat
      241 taaataaatg taaaaattag gagaaatcaa tgtcttatag agtacaatta ataatttcag
      301 aagatgtaga aagtcagcaa tttggcacta atgttattaa taaagtaatt aatccagctc
      361 tttctattaa tgcgccttta attcctactg ctttatcatt ttctgtaaca gctgtggtgt
      421 ctgagataga ggatacagaa aagataaaaa tagtagaaat tgaagtttta aacaagaatg
      481 aaaaacaaat tttttctacg ggtgaagtat cggtaagctt gcctcctcaa gttaacgata
      541 taaactttaa tataaacgcc agaaatgtct tggttgaaga agcaggagag cattatgctg
      601 ttgttaaatt taatggaact gagattggaa ggcaaatctt tgatatcaag gtcaacaaac
      661 cagtggaaaa aaattaagga gacgatacgg atgcttgata tagtaccatc aaatacacat
      721 cggagtggta gcttaataaa ttttgctgcg atttttgctt gcgcatcggt aattctagca
      781 tctccagttg ttgtggatgc aagttctact ccgaaaacta aaaatgataa tcaacttgta
      841 attactacaa aaaatgagat taacacaatt tcaagtaatg atggcaaaat ttttgatgtg
      901 ataaatacgg ttttaaaaag aaaagctgaa atcaatataa aatatgatga agatttaaac
      961 ttgtatttct ttgttatcaa aacaacatct gaattgtttt cctcagatta cgatgttttg
     1021 gatactctcg ataatgtatt atctgactat aaatatatgg ggaaatctgt agtagcaact
     1081 ttgggggaat aaaatgtttg actttaatga atatttatct atttccaaac aaattgaatc
     1141 agatgataaa tataattcta aagaatctta tagaagaact gctgtttcaa gagcatacta
     1201 cagtgctttt aaaaaatctg atgagtattt gaaagaaaat tatgatatta tttataatgg
     1261 aagtagcgga aaaggaagcc accaaaccgt ttggaatttg ttttcaacag ttaaggaatt
     1321 gaatacctta ggaatacata atagtgggta tagaatgtta gagaaaagaa aatgtgctga
     1381 ctatattcct aatgaaacca ttacgaaaac agatatggcg ctgatgaatc gagaagcaga
     1441 aaaaataata aacaaactca cctaatgaat ttcaagcgag tttgtttgtt atttttttat
     1501 ttaatctttg tatctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg
     1561 atcgctacgt gcgtccggca cgtcagtcac gatatcaaac aaaaagtata cacccttctt
     1621 cattctagtt ttcgcagcgg gaattttgaa atagttctta ttagaatagt agagattgat
     1681 taataagcta tcttcgattg ctaaaaagaa aacttctgaa ttccatactt tataaaaatc
     1741 tttgataaat ctattcgaag ggtcaaattt aaaccataat tgtgtttttc cttccatcag
     1801 cataaaagtt cacctcaaaa agagtatacg aacgaatgtt ctttttgtga aggcggaatt
     1861 taatatactg aataatagaa aacaagtact tgtgccaaat tatgtgccaa aaaaattcga
     1921 attcaacaaa atgtaaacaa atagaaaacc ttctatatta agatttcctg caataacaaa
     1981 cacaacagaa gacgtgtaat agttagtcag gaacgtacaa ataacccctg tatcctttgc
     2041 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
     2101 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
     2161 cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa
     2221 cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt
     2281 cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat
     2341 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
     2401 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
     2461 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
     2521 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
     2581 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
     2641 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
     2701 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
     2761 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
     2821 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
     2881 tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt
     2941 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
     3001 accatctact cgatgactgt caggtgctgt gaacacttct tacgtcggca tatttgccat
     3061 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
     3121 tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt
     3181 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
     3241 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
     3301 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
     3361 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc
     3421 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
     3481 aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
     3541 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
     3601 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
     3661 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
     3721 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
     3781 agataacata ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca
     3841 tttaattatg attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt
     3901 taagtttcat gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta
     3961 attttgcata tcaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa
     4021 aaaattaagg agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt
     4081 ggcagctgct tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat
     4141 cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga
     4201 cttgagacaa gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt
     4261 tgagcataca gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag
     4321 agagaagaag ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca
     4381 gaatttctct tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct
     4441 ttcagaagag cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc
     4501 agctttgatg tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca
     4561 agacagtata gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc
     4621 aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt
     4681 tcttacagcg tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa
     4741 gagccataca gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg
     4801 cgactagttc tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat
     4861 ccactttaac attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat
     4921 catccaccga tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa
     4981 tttttcccaa tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca
     5041 gatcgccatc atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat
     5101 ctgcatctac ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt
     5161 ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg
     5221 ctaaagccat gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa
     5281 catcattgaa aagaaaataa aacctaaaga cacctgggga atgtcatttg gctaaatgta
     5341 tgccgctatg ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa
     5401 aatcagcacg tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata
     5461 aaaatctgcg tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca
     5521 gaaactatat cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat
     5581 atgcgacttg tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt
     5641 tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg
     5701 attcttgttg ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag
     5761 taacgaagaa agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg
     5821 acatcggtca tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt
     5881 ttattggtca tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag
     5941 aagctggaaa agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta
     6001 ataccggaac ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg
     6061 gcggactttc ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt
     6121 tgacatcaca aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg
     6181 tacatgaaag tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg
     6241 actcaaaagc gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat
     6301 ttactcttgt ctttatttcc attgattatt gcttttggta atattttacc gtttctccac
     6361 atcgatcaag agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag
     6421 tttatcggtc ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt
     6481 tctgcattag cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg
     6541 aataaagcgt atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta
     6601 attgtcattg tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga
     6661 cagctgattt tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag
     6721 ttccaaactt tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt
     6781 tatttaattg tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc
     6841 gctactgtcg gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca
     6901 acgaggatca gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat
     6961 tttgctgcta cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg
     7021 acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa
     7081 actaaatttt ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt
     7141 aatttataat aattatgact cttaattaaa gtaggtgccc atatgcttgt ttcttttagt
     7201 atggttataa gattttttct gacaatttta ttatcactga tattaacacc tgtattcaaa
     7261 atcatttcag tacaaacagg tatggtcgac aaaccaaatg aaagaagaat aaataaagtt
     7321 ccaatgcctt cagcgggcgg actgcctatc tttatttcat ttgttatatc gactttattc
     7381 ttattccgta aaattatccc tcaatcgtat attgtaccga tcttgattgc ttctttagta
     7441 atcatagtca caggagtgct cgatgataaa tatgagctga cacctaaaca aaaaagtata
     7501 gggatcttga ttgcttctct tattatttat tttgtagcag atatacgtat tgattccttc
     7561 acgcttcctt ttattggata tatccaacta ggctggctta gttttcccgt aacgattttt
     7621 tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg gctggcggca
     7681 gggatttcat tgatcggttt aattacgata ggaatcattg gatatttttt cctccatgct
     7741 tctacagtgt atattccagt tgttattttc tgtcttgtag caagtattat cggttttttt
     7801 ccttacaatt tttatccggc aaagatttat ttaggcgata caggagctct attcctaggg
     7861 tttatgatgg ccgtcctttc actacaaggg ctgaagaacg tgacttttgt ttcatcgatt
     7921 tctttgctga tcgtcatggg agtgccggta acagacacgt tttttgctat cattaggaga
     7981 aaagcaaacc gtgtttcttt ttctacagca gataaaaaac atcttcatca ccgactaatc
     8041 agtttaggct ttactcataa aggtgctgtc ctgacgattt atgcgatggc tttgatgttt
     8101 tctttcacag ctatggtcat gaattatact ggaagaatcg gaacgatcat attgattatt
     8161 gccatgttgt ttgctgcaat tttattattt gaattgattg gtctgatcaa tgagaaacat
     8221 cagtttattt taaaaacatt gcgattttta gggaataaag agtaccgtac gcaagtaaaa
     8281 cagaaatatt tgaagaaatg gaaccgtcat atagatgaat aacaaattaa accagccgat
     8341 ccttattagt attgtgacgg caaacagtga taaaattttt cggacactag ataattttat
     8401 ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca attcacttcc
     8461 cgagtacaaa aaaagactaa cagagtatag gaaatatccc tacatcgata tctattttca
     8521 tgatgaaaat tccggatttg gttatggaca taatcacaat ctgatagcta gcgattctcg
     8581 ctatggcatt atttgtaacc cagatatttt agtggatgaa gagacagtta cagcattggt
     8641 cttgttattg aaagaacatc cggaatgtgc gatgttagca cctaagattc ttaatgaaga
     8701 tggaacaact cagcacctca tccgtgagca gctggctgtt tttgattacg ttttgcgctt
     8761 tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg
     8821 aggattgcct actgatgcta atagttatgt acggatgatc tcaggtagtt ttatggtagc
     8881 tgatattgat aaatttaaag agataaaggg gtttgatgag cgtttcttca tgtactttga
     8941 agacaacgac ttgtgtttga gttttgaaaa agcaggttac aagttattgt acacaccctt
     9001 ttatgcagtt atccattttt acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt
     9061 gtttttgact tctatgatga aatttttcaa taagtggggg tggcgttttt tctgatgaaa
     9121 ccagaaatat tagtagtcgt tgtgctttat cagcaactat tcagtcaatc tccagcttat
     9181 ggaccattaa gcaaagcact tagcgaaaag caaattcagc ttattattta cgataatagt
     9241 cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata tcgtttatca tcatgatccg
     9301 caaaatcctg gtttagcggt ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca
     9361 cgttgtctcg tcactctgga tcaggatacg aggctagtcg acgattattt tgaactattg
     9421 cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc
     9481 cgacaagttt ctcccctata ttcagatcgt tatatcaatc gtacagctca agcagtagaa
     9541 gttgaaggcc caacagctga acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc
     9601 aaatttctaa aaaagattgg cgggtttaat cctgcttttt ctttagattt tttggatcat
     9661 tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg agatccttcc tgtacgaatg
     9721 gaacatgatc tttcggtttt agactatgaa aaagtttcaa gtaatcgata ccgttctatt
     9781 ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga
     9841 cgacaattat tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg
     9901 cggatgactt tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg
     9961 tatagctacc tttaatggag gcagatattt aagagagcag ttagatagta ttttatctca
    10021 gctttcagca gaagatgaag tagtcatttc tgatgatggg tcagtagatg atacatggga
    10081 tatattaact gaatacgcag aaaaagataa gcgaatccag cttttttcgg ggcctcgtca
    10141 aggattgatc gctaattttg gttatgcgat ccagcagacg aaaggagatc taatcttttt
    10201 ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa aagatcactg actattttga
    10261 gcagaatccg gatcagctag tagtagtttc tgatttaatc attgtggaca gcgagctgca
    10321 agagatccat gattcgtatt tttcgtttcg aaattccaaa gaaggatggc ttaataatgt
    10381 gataagaagt cactatattg gtgctggtat ggctttccgg tcttctttaa aacccgatat
    10441 tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg attggtttgc tgggaggaaa
    10501 gaagactggg ttcctaagag aaccgttaac tctgtaccga agacatgatt taaacgcgag
    10561 tgagattgaa acacattcta aattacggca aaaaatttct tggcgtgttc atctgattac
    10621 ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat ccttaatatt
    10681 tccatgacaa atataacaat tgatgagata ataacaaagt gaaatttgaa atcaaaaagg
    10741 agagcagata tccatgaagg gaatcattct tgctgggggc agcggaacac gtctgtaccc
    10801 tttaacaaag gcaacatcga aacaattgat gccgatttat gacaaaccaa tgatttatta
    10861 tccaatgtct actctgatgt tggcgggaat caaagaaatt ctgattattt caacgccaca
    10921 agatacacca cgtttcaagg aattatttgg aaacggaaat gatttaggac tgcagatcga
    10981 atatgcagta caagaaagtc cagatggatt ggcacaagcg tttatcattg gagaagaatt
    11041 tattggagat gatagtgtct gcttggtatt aggggacaat atttactatg gtggcggtct
    11101 gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc gcgacagtat ttggctatca
    11161 tgtcaatgat ccagaacgct ttggtgtagt agaatttgat gagaatatgc aagctttgtc
    11221 gattgaagaa aagcctgctc agccaaaatc aaattacgct gtaactggcc tttattttta
    11281 tgacaatgag gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg gagaactaga
    11341 gattacggat gtcaataaag catatttgga taaaggaaaa ctatccgtag aacttatggg
    11401 acgcggattt gcttggcttg atacaggaac acatgaatca ttgcttgaag catctacctt
    11461 tatcgaaaca attgaaaaac gtcagaactt aaaagtggct tgtttggaag aaattgctta
    11521 ccgtatgggc tacatcgata aggaacaatt gctggccctt gcacagccat tgaaaaaaaa
    11581 tcaatacggt caatatttat tgaatttggc cgctgaataa ggagtcctta cagatgaaag
    11641 taacagatac aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc
    11701 gcggtttctt cacagaaagt tattcaaaag caaaatttgc agaacatgga ttgaaatatg
    11761 actttgttca agacaaccat tcactatcag ctgaagctgg tgtccttcgg gggcttcatt
    11821 tccaaaaagg agaagctgca cagacaaaat tgattcgggt agtcactgga gcagttttag
    11881 atgtagtagt ggatatccgc aaaggtagcc cgacttatgg tcaatgggac ggatttatct
    11941 tatctgaaca taatcatcga cagttgcttg tacctaaagg atatgcccat ggattcgtta
    12001 ctttgacacc aaatgtgaac ttcctttata agtgtgataa ttactataat gcagaagcag
    12061 atgggggcat cgcatttgat gatccagatt tagcgattga ttggccaatt gatccatcaa
    12121 gagcgattat gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt gaagcagaaa
    12181 atccatttgt ttacggagaa atctaagaat taaggagaaa ttttcttatg aaaaacatca
    12241 ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc
    12301 atccagaagt gcatgtcact gttttggata aactgactta tgcaggaaac aaagagaact
    12361 tggccggact tcctagcgat cgcgtagaat tggtcgtagg agatgttgca gatgcagaat
    12421 tagtggaccg tctagtaaaa gaagcggacg cagttgttca ttacgcggca gaatcccata
    12481 acgataattc attaaaagat cctttcccat ttgtacaaac gaatatcatt ggtacgtata
    12541 cacttttaga agcttgccgc aagtatgatg tacgctatca tcacgtatct actgacgagg
    12601 tttacggcga tcttccatta cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa
    12661 aattcactgc ggaaacacca tataatcctt caagtccata ttcttctaca aaagctggtt
    12721 cagatctgtt agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc atttcaaact
    12781 gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt tattcctcgc caagtaacga
    12841 gtatcttaag cggtattcgt ccaaaattat atggcgaagg aaagaatgtt cgtgactgga
    12901 tccatacgaa tgatcattct tcagcagtgt ggctgatttt gacgaaaggc agaattggcg
    12961 aaacctatct gatcggtgca gacggtgaag aagataataa gaccgtgatg gaattgatct
    13021 tagaaatgat gggccaacca aaagacgcat acgaccatgt aaacgaccgc gcagggcacg
    13081 atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg cagcctgagt
    13141 ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa catgaagact
    13201 ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc caaaaatgga caataataca
    13261 gaggaaattt cactgtatta atggaaaaac acatgtgagg caggcgctcg cctgtctctt
    13321 ttttaaaata aaaatcaagg agcggataac catatgtttt tattaactgg aggaaatgga
    13381 caattaggta cagaattgcg ccatttatta gatgaaaaaa atctgccata tgtttcaaca
    13441 gatgcaaaag acttggatat cacagatgct gagaaaacaa tggcttatat tacagaatta
    13501 aaacccgatg ttattttcca ttgcgcagct tacacagcag tagataaagc agaagaagaa
    13561 gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc acttgcagca
    13621 aaagctgtga atgcacaatt cgtctatatc agtacagact atgttttcga tggaaaaaac
    13681 aaaaatgtag aatacgaagt agacgaccaa acgaatccac taaatgaata tggtcgtacg
    13741 aaattattag gcgaaaaagc agtagaagag attttagaag actattatat catacgaact
    13801 tcttgggttt tcgggatcta tggacacaat ttcgtcttca cgatgcaacg cctagcggaa
    13861 acccgcgaca aactgactgt agtaaatgac cagtttgggc gaccaacttg gacacgtact
    13921 ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac catttggtat ttatcatttg
    13981 tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag aaatattgaa agatacagat
    14041 gtagaagttg cacctgttac gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat
    14101 tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg tcataccaac ttggcaagaa
    14161 gcacttgcgc aaatgctgga agctgccaaa aaaatgaata agtaatttcc tttttatcaa
    14221 atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc
    14281 aaaagcgaat caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct
    14341 tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg
    14401 tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg
    14461 acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca
    14521 agctgaagag gacttttcgt tggtgaacga ggattattca ttaaacggac aaaacatgat
    14581 cattgcgtat aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg
    14641 attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt
    14701 catggtcttg cttggattcg aagtactgat gatattaacg aagaggaaaa aagctctatc
    14761 gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca
    14821 tgtgggcgat ttgattttct ttactttggg actgatgatt gctttttatc attatttcta
    14881 caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat
    14941 tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt
    15001 ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt
    15061 agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac
    15121 gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac
    15181 tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt
    15241 tgaagatatc aactattcga ataactctga agtggccctg ttctatcact tttttccaac
    15301 tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact
    15361 gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc
    15421 aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac
    15481 ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga
    15541 tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta
    15601 tgcgattacg cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc
    15661 tgtgttggga acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct
    15721 cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac
    15781 aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc
    15841 tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa
    15901 atccttcaat acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa
    15961 agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga
    16021 agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga
    16081 cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca
    16141 attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt
    16201 taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct
    16261 agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa aggagagtga
    16321 agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt
    16381 tgttgctgac gctagtcttt tttgtttgga cagcaggggt aggttttcca gcaactttgt
    16441 ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa
    16501 atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca
    16561 ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc
    16621 ctttgattat tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg
    16681 gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt gtatttacag
    16741 agatccaagt accagaagga caaagcatct gggctccagg caattcgctt tctgatattt
    16801 ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat
    16861 ttgccagttc ttttgaacga tggtatgttc gaatcatcgg tgggatagca gtcgttgcag
    16921 cctttttagg tatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg
    16981 cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgcct
    17041 acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag
    17101 tcaatttgtt taccggtcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa
    17161 aatctgaaca agttttacta ggaatgaaaa catttatctt ccagcgagga aaccaatttt
    17221 taggaaacca caatagttat aattctattt tgaatatcta tggattgata ggatttggga
    17281 ttgctgcttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca
    17341 acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg
    17401 atacattgac ttcataccga tgggttccga ttatttatat cctgttggct atggccgttc
    17461 atcgatatga tgaaccagaa gttacggagg aattctatgt tatggaagag gcatatgacg
    17521 atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat tcttccagga
    17581 tccaacgcta tcattgatag gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct
    17641 tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct
    17701 tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa
    17761 atggcacttg actgtaattc actgaaaatc gcttggagga acacaaatgt ttaaagattt
    17821 gtatttattt atgaaggaca tttatcagaa cagaaaatta ctgctacagt tttcgatcaa
    17881 tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc
    17941 gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat
    18001 cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt
    18061 ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa
    18121 agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca
    18181 tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc
    18241 aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac
    18301 ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt
    18361 gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg
    18421 tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc
    18481 atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt
    18541 cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc
    18601 agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca
    18661 aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt
    18721 aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa
    18781 ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc
    18841 attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct
    18901 ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta
    18961 aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt
    19021 gaatttgctg acatcggcga ccatatttat caacctgtaa aaacctattc aagtgggatg
    19081 ttcgttcgtc tagcttttgc tgtagcgatc aatgtgaatc ctgatatttt gattgtggat
    19141 gaagcattgg cagtaggaga tcttgaattt caactaaaat gtatggaaaa atttacagaa
    19201 atcaagaact cgggaaagac gattttattc gtttctcatg atattagttc gattcgccgt
    19261 ttttgtgatc gttcctattg gttgaaaaat ggaaaagttg tcgaatatgg tgatacgatg
    19321 gaagtaacga cgaattacga aaactttttg aagaaaaaat cagttaaaac agtagatcga
    19381 aataaaaacc aagtagaacg cttcgcagct ccagatatcg tagacgtgac gaaagccgaa
    19441 ttgttggata aagataaaaa tccagtggat atgctggaac aaggagaaaa actatacgtt
    19501 aaagtaacct atgaagtaaa agacgataca atcaaaaatc cagttttagg aattgctctg
    19561 cgtacagtcg acaattttta tgtgtgcgga ctcaatactc tactagatgg tatcaagatc
    19621 ccttggaaaa aaggtgagaa tatcttttat ctggagtatg aaaagttagg tttgttagct
    19681 ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatac
    19741 aagacaaaat acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc
    19801 ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct
    19861 agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt
    19921 agagtttggt cctggaaacg gccgattaac gaaacatttg atcggagcaa aacaatgtga
    19981 agtgagcatt gtcgagctag ataaggaatt gttcgatttt gtcagcgagt ttgcccaaga
    20041 tggattttat ggagatatcg aaagttttga atgggcaaac tattatgctg gacaaacatt
    20101 tgattacgtt ttgtttgccg atgttttaga acatttggta gatcctggaa aaacgttgaa
    20161 gaaagttaga gaatttttga atgaagaagg agaaattcta attacattcc ctaaccttgc
    20221 gcataattct gtcatgattg atttgttcaa taatcaattg ccatgggctt cttatggttt
    20281 gctagatgaa acccataatt ctttttatac tcatgatggc ttccaaaaag tttttgaaaa
    20341 agcaggatta tacatcaata ttgaagatta tttgtactta gctgtaggtg atacagaatt
    20401 aaaatcaact tatgaagaat tgccggaagc tgtacgttat gatttcaaaa tgcgaccatt
    20461 tggcgaagtc taccaatact tcttctcatt gatgaaacat ccagtagcac aaagttccat
    20521 tgcgcagccg caaaattcta attatgtgaa agtattagaa gtaatccagc aaacgaaaca
    20581 agatgaaata attcaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag
    20641 tttccatgta tcagaattag cagaacgaat ggtgtttcgc ttcgcgcagc aacctagctt
    20701 tatcgaattc agcgcagaag ctgctggaga aaaactcaca tttatcgatt caaatgctgt
    20761 agtcaaaaca gtaaatgatt gttatttgtt tgatggtaaa gatttaccag agttcttttt
    20821 aacggatatt gcagggaaag aagtgactat ccattgtcac tatcgtttta tcggagaatt
    20881 gacaccaaca atgaaagaat tgctggaaac aatccgtcca atggctgaag ttacaaagca
    20941 gctttctgag aaaaatgatg aattagaaag agaaaatcat tatcttattg agaataatac
    21001 aagattagat cagacgttga aaacaaccat aaatcgctat tgcacgttgt tggaaagtga
    21061 tgaatggaca atcaaacata gattagggcg acgtaagaaa gaaacatcga aaaaaatcca
    21121 agaaaaagag ctttctctat gtattgacga aaaaatctgg gatgcagaaa caaagatatt
    21181 gaaaatcatc gggtggggaa tcgctaattc ggatcgccaa ccattgtcct ataagttatc
    21241 agccgaccaa tcaccatttt ttgaagcaat tcctgtttta cgagaagaag tcaatcaagc
    21301 aaaacagcta gctgaaggag caaaagcagg atttgaactg cggatccttt gcgagaagga
    21361 acgttcattc ttagttgaag cagtggctca aaatggtcaa tcatggatca ttgaaatgta
    21421 aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc
    21481 gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg
    21541 gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt
    21601 gctgaagcaa cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag
    21661 acccaaagcg gttttaaggt acgtttatca gggaaaatga gaggaaaagc aatcttagac
    21721 ttccaaacag caagacatca aaatggcata gctgtgaaat taaacggtaa atatccttat
    21781 gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat
    21841 tatgcaagga ctcatggcgt aaaaaaagca atccgccggg tcaaactgga attgaaacca
    21901 ggatcgatcg actatgcaga gtggatcagt cgtcatgaga aacctgatct gaaagagcaa
    21961 aggaaagaag tacaaggatt tgcctaccgc ccattgattt ccatacttat gcctgtctat
    22021 aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tacttgatca aacgtatgat
    22081 cactgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtcta
    22141 gaatcctatc aagcaaaaga tgatagaatt aaagtcgttt tccgacaaga aaatggacac
    22201 atcagtctgg caactaattc tgcattggaa atggcagaag gagagtttat agcgctctta
    22261 gataatgatg acgaattacc tccatttgcc ttatatgaag tggcaaaagt gctaaacgta
    22321 catccagaac tggatttgat ctacagtgac gaagataaaa tcgatgcaga tgggaatcgc
    22381 tttgacccgc acttcaaagc agactggtct cccgatacat tgatgggaaa taattatatc
    22441 tctcacttag gcgtatatcg gacaagcatt gttaaggaac ttggcggttt ccgtaaagga
    22501 tatgaaggct ctcaagatta tgatttggtt cttcgtgtga cagaacaaat tcctgcagaa
    22561 cacatttacc acattgaccg tgtcttgtat cattggcgga cgatcccggg atcaactgca
    22621 agcaatggag aagctaaaag ctacatctat gattctggag tgaaagcatt gacagatgct
    22681 ctatctagaa gaaatatcaa gggaagcgta catcctggca gaatttctgg tttttatgaa
    22741 atcacttatg atgttttgca agaagacttg gtcagtgtga tcattccaac taagaatggc
    22801 tatgaagatc taaaaacttg tgtggattcg atcatcgaaa aaacgtctta tccaaattat
    22861 gaaatcatta ttgcagataa tggaagtaca gatcctaaaa tgcaagaatt atttgcagaa
    22921 tacaagcatc agctgaaaga ccgatttatc gtcgaattaa ttgacattcc attcaattat
    22981 tcacgtatca acaatctggc agcagaaaaa gcaaacggca aatacttttt attcttgaat
    23041 aacgatacag aagtgattga acctgactgg atgacagcga tggtttctta tgcacagttt
    23101 gatcgaatcg gctgtgtagg agcaaaatta ttttatccgg atgatacaac gcagcatgca
    23161 ggtgtattat tagggatcgg tggcgttgca ggacatgcat tgaataacta tgatcgaaca
    23221 cattgtggtt actttggccg attggtcatt gacgtcaatt acttagcagt cactgcagct
    23281 tgtatgatgg tcaaggcagc tgacttcaat gctgtcaatg gatttgatga aatattagaa
    23341 gtagcattca acgatgttga cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc
    23401 tatgcacatc aagcagaatt gtaccatttc gaatctaaat caagaggtta tgaagataca
    23461 ccagaaaaac aaagacgttt tgctggggaa atcaaaaaaa tgcaagataa gtggccttct
    23521 tatattgctc atgacccatt ttataacgat aatctaacta aagaaggaat cggcgatttt
    23581 tcgttgagac ctgattgatt aaaagaactg tattttcatt tttattaatg aaaatacagt
    23641 tct
//
LOCUS       DAZUMO010000037        23596 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000037 DAZUMO010000000
VERSION     DAZUMO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.037"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..628
                     /locus_tag="ACG8ER_001581"
     CDS             2..628
                     /locus_tag="ACG8ER_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050163.1"
                     /translation="MQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLL
                     DHSDITVAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLA
                     ILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFS
                     FFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(762..887)
                     /locus_tag="ACG8ER_001582"
     CDS             complement(762..887)
                     /locus_tag="ACG8ER_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS5050164.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1021..1914)
                     /locus_tag="ACG8ER_001583"
     CDS             complement(1021..1914)
                     /locus_tag="ACG8ER_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS5050165.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2096..3022)
                     /locus_tag="ACG8ER_001584"
     CDS             complement(2096..3022)
                     /locus_tag="ACG8ER_001584"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS5050166.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3107..3868)
                     /gene="pflA"
                     /locus_tag="ACG8ER_001585"
     CDS             complement(3107..3868)
                     /gene="pflA"
                     /locus_tag="ACG8ER_001585"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS5050167.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4112..6343)
                     /gene="pflB"
                     /locus_tag="ACG8ER_001586"
     CDS             complement(4112..6343)
                     /gene="pflB"
                     /locus_tag="ACG8ER_001586"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS5050168.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6620..9070)
                     /gene="parC"
                     /locus_tag="ACG8ER_001587"
     CDS             complement(6620..9070)
                     /gene="parC"
                     /locus_tag="ACG8ER_001587"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS5050169.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9082..11127)
                     /gene="parE"
                     /locus_tag="ACG8ER_001588"
     CDS             complement(9082..11127)
                     /gene="parE"
                     /locus_tag="ACG8ER_001588"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS5050170.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11293..11727)
                     /locus_tag="ACG8ER_001589"
     CDS             complement(11293..11727)
                     /locus_tag="ACG8ER_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS5050171.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11854..12492
                     /gene="plsY"
                     /locus_tag="ACG8ER_001590"
     CDS             11854..12492
                     /gene="plsY"
                     /locus_tag="ACG8ER_001590"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS5050172.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12624..13499)
                     /locus_tag="ACG8ER_001591"
     CDS             complement(12624..13499)
                     /locus_tag="ACG8ER_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5050173.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVIRYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13581..14372)
                     /gene="codY"
                     /locus_tag="ACG8ER_001592"
     CDS             complement(13581..14372)
                     /gene="codY"
                     /locus_tag="ACG8ER_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS5050174.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14390..15790)
                     /gene="hslU"
                     /locus_tag="ACG8ER_001593"
     CDS             complement(14390..15790)
                     /gene="hslU"
                     /locus_tag="ACG8ER_001593"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS5050175.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15804..16352)
                     /gene="hslV"
                     /locus_tag="ACG8ER_001594"
     CDS             complement(15804..16352)
                     /gene="hslV"
                     /locus_tag="ACG8ER_001594"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS5050176.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16389..17294)
                     /gene="xerC"
                     /locus_tag="ACG8ER_001595"
     CDS             complement(16389..17294)
                     /gene="xerC"
                     /locus_tag="ACG8ER_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS5050177.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17492..18868)
                     /gene="trmFO"
                     /locus_tag="ACG8ER_001596"
     CDS             complement(17492..18868)
                     /gene="trmFO"
                     /locus_tag="ACG8ER_001596"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS5050178.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18837..20915)
                     /gene="topA"
                     /locus_tag="ACG8ER_001597"
     CDS             complement(18837..20915)
                     /gene="topA"
                     /locus_tag="ACG8ER_001597"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS5050179.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21037..21894)
                     /gene="dprA"
                     /locus_tag="ACG8ER_001598"
     CDS             complement(21037..21894)
                     /gene="dprA"
                     /locus_tag="ACG8ER_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS5050180.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21950..22717)
                     /locus_tag="ACG8ER_001599"
     CDS             complement(21950..22717)
                     /locus_tag="ACG8ER_001599"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS5050181.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22710..23570)
                     /gene="ylqF"
                     /locus_tag="ACG8ER_001600"
     CDS             complement(22710..23570)
                     /gene="ylqF"
                     /locus_tag="ACG8ER_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS5050182.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6133 a         5103 c         4065 g         8295 t
ORIGIN      
        1 aattcaacgt agaaccacta attttttaca tttaggctct ttttttgcaa ctttgacgat
       61 cttatttttt ctatgttatt cataccttaa gacctcacat gcgccttcta gtgcattagg
      121 ctggttgctt ttggatcatt ctgatatcac agtagccgaa gaaaatccgt tttttttgat
      181 tttgtcttct ctttgcggct tactgttttc tgtttatttc ggtgcctatt tttttcatcg
      241 acatcctggt acgttaaacg aagcttcaag cgtattgatt gcatttaaaa cgttttcttt
      301 tttagcaatt cttttttatt cgtttaatca caatacgctt ttcttcttta tcatgaatgg
      361 tttagtcgtg attttatcca tttttactta ttttattttt tcactcattt ttagccaatt
      421 gaaatgtccg ctcttctttc gattagttcc tgtcacctgt tttatttaca gcttgttttc
      481 cttttttgtc cttgctttaa tgccactgac caacccttcg acgatccaag tcgcaggaaa
      541 tctgttggat atgctcttca tcgtcagtct aggtgtattt atgtggcaaa gaaaaaacaa
      601 acaaaataaa aagcattttg aagcctgact attacgactt cataaatgct tttttagttt
      661 taaacacttc aatctaaaag aataacccaa gattggataa attattaatg actcgccatt
      721 cgtttcttag tgattctgga ttatcaaatg gacttcctaa tctaatcagc tagttcttcc
      781 cataaaatgc catacttgac tgaatcataa tattgattat ttaaaaatct aacttttctt
      841 atcactactt cctttgtcat tccacatttc tcaccatttt tttgcattcc tttgtttcca
      901 gaccatgtag tgaatctaat aggtggtaga taggcaaaat actcaaataa ttcttttaac
      961 cataaaggaa gagaaaggct cctcctgcaa gagtcagtat aatttttgct tactgctctc
     1021 ttaggtttta ctcactaaaa ctgttccata ttctttgata taagaaaatg catgcggtat
     1081 aaatttcttg ctcagctcag aaaagatttg tcgtttaaga aaggccattt cctccattgt
     1141 caatgaagga tccaatgttg gtaattcaat ctcaaaattg tttttttcga caataggtaa
     1201 ttctaatgtg aatgattctt cttcctttat agtattcgtc acaagtagag actgatggaa
     1261 aatactaggc cggcgatttt tatatttatt cttacgtatc ctgtcaacga taacttctat
     1321 ttgatcaaaa aagtacgatt catttacgtc tccaataagt tcggctaatt ctttgaatgc
     1381 cttcgttaca ggaagctgat tcttctgtac aaaaaagtaa ttagccaaag taagcgggaa
     1441 gtgttctata gaggaaagtg tgataaaacg gtacttctca tttcctgcag ttgtcctcag
     1501 caattctgtc tctcggctgt ttcttactcc ataaaaatac gcttctttcc gttcatcttc
     1561 gaaacaatac gcccaaataa cttctcccat cgctaatttt tgttcatcaa cagagagtct
     1621 cttcaattgt tcagctatgg tttcagcagc cgaagtggct tgttgcaagt aatctttagg
     1681 atcaaatatt ggaaagttca aatgatatct tcgttcttcc ctcttgatta atcctgcatc
     1741 gatcaataaa tcaagaaaat gggaaaattt attttgcgaa aattcttttt tcaattctct
     1801 tagaatcgtt tcttgttctc gatgttcata tagataatcg gtcaatgaag aaaaaagcgg
     1861 atcatcggtc agtttttgat aaaagttttc cttatttttt gaataatagt tcatatatgt
     1921 tctcttttct atccgtttta ttttacttaa aaggctttta cgtaaaaagg aggaagaaca
     1981 agagtcataa ttgactgtta ttctcctcca tttcttcggc ctttagtatg ttctatgttt
     2041 ctttttatat caatcgctta tccattcatt ttgaatgctt catactacca tttgattagc
     2101 taaatacagc cgtcaattga ggtacgactt gttttttacg tgaaacgaca cctttaagca
     2161 aagcacggtt gttggtcaaa gttgtttgga aagcttcttc tactttttct tttcctttac
     2221 cagcaacaag taattctgaa tcactgtcta agatgtttgt gacaactaat acgaacagat
     2281 cgtagccatt tgctttcatt tcatcttcca ttgctgcttc tagctctgct tgtcgatcca
     2341 tcacttcatt aaggtccact gtattgactt gagcgatacg aacgttggaa ctgcccattg
     2401 ggaaactttt tgcatccaaa tcaagaagaa ccgcagctga tttatcactt agatttgttc
     2461 ctgctttcaa catttcaagt ccataagtgt ttagatcgac ttcagcgatt gctgcaagtt
     2521 cttttgcagc tgcttcatct tcaggcgtac atgttggtga tttgaacaac aatgtatctg
     2581 aaatgatagc tgagagcatg attcctgcga tttctttagg aattgttact tggttttctt
     2641 tatatagttt taaaataatc gtacttgtac atccaactgg ttctgcacga tagtacaatg
     2701 gattagctgt ttcaaagttt gcaatacgat ggtggtctac tacagctaaa atatttactt
     2761 cgctgatatc tgaaatactt tgttggaatt cattatgatc cacaagcatg acatctgcat
     2821 tttctgcttt agctgactct actactcgtg gtgcagatag ctgaaagtgg tctaatgcgt
     2881 attgtgtttc ttcattaggt gtacctagag caactgcttc tgtttcttta cctaattcat
     2941 tttgcaagtg tgaaaacgca atggctgccc cgattgcatc tgtatctgga ttttggtgac
     3001 caaaaactaa aatttttgac atgtcattcg ctcctttgtg atttctccta tttaataata
     3061 gcaaaaaaac ggaaagaagc aatacttatc ttctttccga tgcgacctat cgcgttaaat
     3121 attttgtata ctcttctgta tgaagcaatc gtttagcatt ttcgacacgt tcttttgtag
     3181 gtggttcgat cccttctaat ggatatttca aaccaagtgt ttcccacttg tattttccca
     3241 tcgtatgata aggaagaatc tctactctgt ctacatttga caaggtttga ataaagttgt
     3301 ctagtcggat caaatattca tcataatcac ttcgctcagg aactagtaca tggcgaatcc
     3361 aaaccggttt gttgattttt gacaaatatt gagccatttc taaaatattt tcattgtccc
     3421 aatgcgttag ttttttatgc tcttcattat cgatgtgttt gatgtcaaat aacagtaaat
     3481 ctgtatactt catcaattct tgaaaacgtg aaaagaaagg ttcttcatac gtaaaaggtt
     3541 ttccgcatgt atccaatgta gtatgcatct tccgctcttt tgctttttta aatagttcga
     3601 tcaaaaaatc gatctgcaat agaggttctc caccactaac agtaatacca cctttatctc
     3661 cccagtaagg tctgaagcgt tctgctttat ctaaaagttc atctgctgtg tattctttcc
     3721 ctccgccaat attccaagta tctggattgt gacaaaattg gcaacgcatg cggcatcctt
     3781 gcataaacac gacaaagcgc agtccaggtc cgtctactga accgaaagtt tcgatagaat
     3841 gaacatagcc gattgttttt tcttccattt aagttcacat cttttctttg atttcatttt
     3901 aagtacaagt tagctgtcga agcgaagctt tctgaacaaa atttttcgca attgccgctt
     3961 cgttatgtgt aaaatttaca gggacggaac atacaagtcc tgcccctgta aatcgtttct
     4021 ttcaaataag catactgatt attcgatttt gatgcgatgg ctattcaacg tcctttcgac
     4081 gctaagtcga aaaacttttt tcacgaactt tttacattct gtcgtgtgaa gttctcatca
     4141 agacatctaa ttgttgttct cttgtcaagt cacggaattt aactgcataa ccagataccc
     4201 ggatcgtcaa gttcggatat ttcattggat tttccattgc atcacgcaat aaatcattac
     4261 tgaatacatt gacattcaaa tgataagctc cttttgagaa gtatccatct aagacattac
     4321 gcaagttttc gattcttgta tcttgttctt ttccaagacc tgaaggattg attgtttggg
     4381 tatttgaaat tccatccaat gcacagccgt aattcatttt agctgttgag ttcaggctag
     4441 ctaataaccc actttgttcc gcattgtaac ttggatttgc tcctggcgca agtggttttc
     4501 cagcttcacg gccatcagga gtatttcctg tatttttacc gtaaacaaca tttgaagtaa
     4561 tcgtcaataa tgaagttgtt ggttttgagt tgcggtaagt tttatgacgt ttgatttgtc
     4621 ccatgaagta ttccaagatc catgaaacta attcatctgc acggtcatca tcatttccgt
     4681 atttagggaa gtcgccatcc acttcaaaat caactgctat tcctttttca ttacggattg
     4741 cttttacttt tgcatatttg atcgctgaca agctgtctgc cgcatgagag atcccagcaa
     4801 tacctgtagc aaatgtgcgt tgcaagtcag aatccatcaa agccagctga gctgcttcat
     4861 aagaatattt atcatgcata taatgaatga tgttcaacgt attgacatat agttctgcta
     4921 accaatccat catattcatg aagttatgtt tcacttcttc aaagtctagt atttctgatg
     4981 tgattggacg atattctggt ccaacttggg cgccagtcac ttcatcgact ccgccattga
     5041 tggcatataa tagtgtttta gccaagttag cacgagcgcc aaagaattgc atgtcttttc
     5101 cgacaactgt tcctgataca cagcaagcaa tcgcacaatc atcagaaccc cattgttcac
     5161 gaagctgatt atcattttcg aattgaatcg aagaactttc gatagcgatt ctgctagcat
     5221 atgttctgaa tccttctgga aggtgttcag aatacaaaac agtcaagttt ggttctggtg
     5281 aaggtcccat atttgttaat gtatgaagca gacggaaatc attctttgta actaatgaac
     5341 gaccatctaa tcccatacct gcaatggata aagtagccca gatagggaat cctgagaata
     5401 attggttata ttcaggtgta cgcgcgaatt tgaccatacg taatttcatt actaagtggt
     5461 cgatcaattc ttgtgcttct ttttctgtga ttactccatt atccaaatca cgttggatat
     5521 agatatcaag gaatgctgag atacgtccga ttgacatcgc agcaccattt tgtgatttga
     5581 ttgctgctaa atatccgaag tatagccatt ggacagcttc ttttgcattt gcagctggtt
     5641 tagaaatatc atagccatat ttcgcagcca tttctttcat ttgcgctaat gcacgatatt
     5701 gttcagtgat ttcttcacgt aaacgaatca cttcttcact cattgtatga taaccagtca
     5761 tatcatgatc ttttttcttt tgttccatca aatagtcgat tccatacaat gccacacgac
     5821 gatagtcacc gatgatacgt ccgcgaccgt aagcgtctgg aagccctgta atgattttgt
     5881 ttttacgagc agcacgcatt tcagcagtat aagcgtcaaa tacaccttga ttatgtgttt
     5941 tgcgccagtc agtgaaaata tgtgtcaatt catcgtttgg tgtatagcca ttgcttttta
     6001 gcgcattgtt agccatattg attccgccga acggcatgaa tgcttgtttc aaaggtttgt
     6061 ctgtctgtag accgacgatt ttttccaaat ctttatccaa atagcctgca tcgtgtgaag
     6121 taatagttga tacgatttct gtgtccatat catagacacc gttacgctcg aattggatat
     6181 catttaactt ttgcaattca tcccataatt tttctgttgc ttctgttggg ccttccaaaa
     6241 agctgtcatc accgcgatat tcagtgtagt tattttgaat aaagtctcta gtgtctacgc
     6301 cttctttcca tttggtacct ttaaatccat tccattgctc cattgtatgg acctcctaaa
     6361 tttctgtttt atgtaacagt ttttacgtga ttagtataac acagcaccct atctttgcaa
     6421 gggttttctt gttttttatt taacaatttt tatgttttat ttacatatat ttaaatcaac
     6481 gttgacatac caacatttaa gcattgtgaa cactttcatc tttttttgca aacgtataag
     6541 gatttattaa aaatgaatga aaaagagacg ccacctgtat taatacagat ggcgtctctt
     6601 cattaaaata ctacaacagt tacgactcga tgaaaatctc aggagcatcg ataacttcga
     6661 tgacttctcc accttttttc tcatccatcg caaatgatcc attcgatgtt cgatcaccta
     6721 tagggaaatc agttgggtca atcgttaact gctgcccttt ttgattcatc aaggttattt
     6781 cttttgatct tccttccgac atatagataa tacggtgcgg attttttttc agctcacgca
     6841 tgaccgtcac tccccgcttc gcacgtgaag tttggctgat ttcttgagca agcatgcgtt
     6901 taacagctcc tcgctgagta acgattacga tcggcgtatc accttctgct aacacaagca
     6961 gcccgttgat aatcctatct ccatctttca aattcataga cttcactcca gctgcctttg
     7021 ctccaacaac gggaacatct gttaaaggat agcgcaaacc cattccttga tgcgttacta
     7081 agaaaacatc taagcctggc tgatcttctt tcagatacac agcaatcagt tcatcttctt
     7141 ccgcctttag cttcatacag ctgagcggac ggctcttata ggtgcgccat ggttcgaact
     7201 ctgtcattcg tgtttgcttg atcaatccat tcttgctgat gaatacaaat gttttctctg
     7261 cttgaatttt ttgatatggg aaaacagcta aaatcgattc gtctacagac agattggaga
     7321 ttgtttgaga aatgtgctca ccagcatctt tccatttcaa atcgggtagt tcatggacag
     7381 ggcggtagat cacatttgct ttatttgtga tcagaagaac atggtccaat gtactcaatt
     7441 cacctgagta tagcaagtag tcgccttctc gcatcccgat ttcttctggt ttggaagcac
     7501 tataagaacg aaggcttgtt cgcttcacat agccttctcg cgtcacactg acaattacgt
     7561 cttcttgtgc cactaagact tgcgtatcaa ttttgatttc ttcgatttcg tcttcgattg
     7621 tcgttagacg agcggaagaa taattttttt tcacttctcg caattctttt ttcataacag
     7681 agaaaagctc tttgtcattt gaaaggattt tattcaactc tgtgatttgc gcaatcaagc
     7741 tttcggattc cttttgaagc tctgtgatat ccgtgttcgt caaacggtat aattgcaatg
     7801 tgacaattgc ttctgcttgt tcttctgtaa attgaaagac gtcgactaaa ttctttttgg
     7861 cgtctttttt gtctttactt tcacgaatcg ttgcgattac ttcatccaga atcgataaag
     7921 ctttcatcaa tccttctacg atatgctgtc ttttttgagc tttggctaaa tcgaattgac
     7981 tacgttttgt tatgacttgc tttcgatgct cgatataact tcttaaaatg tctttcagtc
     8041 caacttgatg cggagtcata tggtcgatag cgaccatatt gaaattgtaa ttgatctgta
     8101 gctcagtgtt tttgaacaaa taattcaaaa ttccttgagc attggcgtct tttttcaatt
     8161 cgacgacaat ttgcagacct gttctatcac tttcatctcg tacttcagct attccatcga
     8221 ttttcttatt caaacggatc tcatccattt ttttcaccaa ggtcgcttta tttacttcat
     8281 atgggatttc tgtgatcacg atttgctgtt tattaccctt cattgcctct atctttgttt
     8341 tcgaacgaag aataactttt cctcgtcctg tttcataagc ttttttgatt tcatcttttc
     8401 cttgcaagat ccctcctgtt ggaaagtcag gtccaggaat atattccatc aatttttcaa
     8461 ggctcgcttg tggatgatcg atcatataga ttgtgccatc gatcacttca gctaagttat
     8521 gagtcgggat ttctgttgca tatccagcag atatacctgt cgatccattg acgagaaggt
     8581 ttgggtatcg agctggaagg actgttggtt ctttttctgt atcatcaaaa ttccacacga
     8641 gatcgactgt ttctttctcg atatctgcta gcatctctcc acttaacttg gataatctgg
     8701 cttcagtata acgcatcgca gcaggtggat caccgtccat actcccgtta ttcccgtgca
     8761 tttcgatcag tacttcccgc aacttccagt cttggctcat gcggaccatc gcttcataaa
     8821 tactgcggtc accatgaggg tgataattcc ccatgatatt tccgacagat tttgctgatt
     8881 tacggaatcc tttgtcatac gtattgccat ctttgttcat ggcaaataat atccggcgtt
     8941 gcactggctt caatccatca cggatatcag gcagtgcacg ttcttggata atatatttgg
     9001 aataacgtcc gaagcggtca cccatcactt cttctaaagt taattcttgt acttcttgtc
     9061 gattttccat caggtgacct cctattctac ttcaaataac tgattgtttt catttttatc
     9121 tgctcgttct tcatctaata aatcattaga aactgaagga gagatttcag tatcctcttt
     9181 tttatctaag atactgccat cttcttcgag cgtaaattgc acgtgatttt cgatccattt
     9241 tcttcgaggt tctactttgt cacccatcag tgtggttact cgacgttcag cttgcgcagc
     9301 atcatcgata cggacgcgga tcaacgtacg ggaagtcgga tccatcgttg tttcccacaa
     9361 ttgttccgca ttcatctctc caagtccttt atatcgctgc agcatatatc ctttgccgac
     9421 ttttttaatc attgctgcca attcgtcatc tgtccacgca tattcaatga cttgcttctt
     9481 tccttgtcct ttggatactt tatataaagg cggtaacgcg atatatactt tcccagcttc
     9541 aatcagcggt ttcatataac gataaaagaa agtaagcagt aatacttgaa tatgggcgcc
     9601 gtctgtatcc gcatcggtca tgatgattac tttgtcgtaa ttacagtctt cgatagaaaa
     9661 ctcaggaccg actcctgcac caatcgtata gatcatcgta ttgatttctt catttttcaa
     9721 gatatcttgc attttagctt tttcggtatt gatgactttt cctcgtaaag gtagaattgc
     9781 ttggaacttt ctgtctcttc cttgtttagc agatccgcca gcagaatccc cttcgactaa
     9841 atacaattca tttttcttag ggtttcggga ttgtgcgggt gtcaattttc ctgaaagaag
     9901 agattcgcct tttttacgct tcttcccatt tcggctttct tctctggctt tacgtgctgc
     9961 ttctctcgct tctctggctt tgattgcttt acggaccaac atctggctca tttcgctatt
    10021 ttcctgcaaa taaaagccca tttgttcact gatgacgtta tctactacgg tacgagcaac
    10081 tggcgtacca agtttttctt ttgtttgccc ttcaaattgc agaagatttt caggtacacg
    10141 gatagaaagt acagcagcta acccttctct gaaatcacta ccttccagat tcttgtcccg
    10201 ttctttcagc aaaccaactt ttctcgcata ttcattataa gatttcgtca tcgccgcttt
    10261 cataccagct tcatgggttc ccccatcttt tgtccgaacg ttattgacaa aagataaaac
    10321 attttctgaa taaccgtcat tgtattgata agcgacctct acttcgatac cttctttttc
    10381 tcctgaaaaa taaacaactg gtgtcaatgt atctttttct tcattcagat aatcaacaaa
    10441 ttctttgatt ccttcttcgt aatgaaaaat ttccttgact ggttcttctc cacgaagatc
    10501 agaaagagag attttaacgc cttttagaag aaaggcagat tctcttaacc gttcggctaa
    10561 aatttcatat gaaaatttcg ttgtactgaa tattgtatca tctggtaaaa aatggacagt
    10621 cgtcccgttt gctttttttg tttttcctat ctttttcaaa gtaccgtcag gtttaccgcc
    10681 gtttttaaat ttctgctgat actctacgcc atctctaacg atcgttacaa ttagccattt
    10741 cgataaggca tttaccacac tggctcctac accgtgcagt ccaccagaag ttttgtaccc
    10801 tccttgacca aactttccgc cagcatgcag cacagtaaaa atcacttcaa ctgttggtat
    10861 tccggaagca tgcattccta caggcatccc acgtccgcta tctgcaacag ttatactatt
    10921 gtcttcatgg atggtcacat caatctcact gccataacct gataatgctt cgtcaactgc
    10981 attatcgacg atttcataga ctaaatgatg caagcctcgg ctgtcagtgg atccaatgta
    11041 catccctggt ctttttctaa ctgcctctaa tccttccaat acttggatgg acgagtcatt
    11101 gtattcgttg tttacttttt ttgccaaggc aaacgctcct tcaataatac tttgcggcaa
    11161 aaccgcacca cgattatcat actagaaaag gtcgtgaaaa aaaagagggt agacgagaaa
    11221 tcaattttaa ttatctccta accgcttttt cgacattccc ctctatcgat catctcttct
    11281 ttgtggtcat ttttattttt ggttttcttt caacttacca agttcaatct taatacaacg
    11341 atccattacg atatctgtac gtcctgcttt tcttagcatc tcagctgctt ctttgttttc
    11401 aatccctaat tgtgcccaga acactttagc atcagttttc aaaaaatcac ctgctatttc
    11461 aggtaaaaat tcactccgtc taaatacatc tacgatatcg attggatcat cgatttctgt
    11521 caaattcgca tagactttct cgcctagaat ttcttcacca gccaataatg gatttactgg
    11581 tatgatttta tatccattgt cttgcaatac ctgtgcaacc atgtaacttg ttcgttctgg
    11641 tttattgctc aagcctacga cggcaatatt ttttgcttct tccaaatagt tgaaaatcgt
    11701 ttcttgtgtt ggatttttta cagacatttc tgccacccct tgcttttctt tttattccat
    11761 ctattatact tgtatctttc attttttcaa ttgttcctgt tacctattca tgctaaaata
    11821 aaggatagtt ttcaacttga aagagggtaa aaaatgaaat tgatcttatt acttatttta
    11881 gcatatcttt taggttctat tccttccggt gtctggattg gcaaattgtt tttcaaaaaa
    11941 gatatccgac aacacgggag cggtaatact ggaacgacca atacatttcg tgtattaggc
    12001 aaaacagccg ggattgttgt cctgcttatg gatattttaa aaggtacatt ggctacttgt
    12061 ttgccgatga tcttccacct ttctgggatt aaccctcttt ggtttggggt atgcgccatt
    12121 cttggccata catttccgat ttttgctggg ttcaaaggag gaaaagctgt tgcgacaagt
    12181 gcaggaatgc ttttaggatt taatcctgta ttttttgttt attcttgtat catctttatc
    12241 gttagtttat tttgtacaag tatggtgagt ttgagcagta tgatcagtgc agtattgatc
    12301 actttatcta cgatcgtcct tccttatgta gctcctgtga ttttggctaa gcccaattgg
    12361 ttactgacga tcattgcctt tttagtttct tcctttatct tttatcggca taaagacaat
    12421 atcaagcgta tcagaaacca tactgaaagt cgaatcccgt ttggtttgaa tagcagcaag
    12481 aaaaaatcct gatacttttt cattccaaag agataaaacg ggagtgtgac aaatcgatgt
    12541 cacactccct tattacttac aggatagctt ttttacacac cttggtaaac ggtgttccct
    12601 gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt cttgaagatt
    12661 tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact actgttcacc
    12721 gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt tggataagga
    12781 gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg ggaagatttt
    12841 tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc atcttgctca
    12901 aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt ttgatccata
    12961 tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga aaatgccaga
    13021 tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg atgtccaccg
    13081 atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac cgtgatgccg
    13141 tctcctccta attcatacct gatcactaat tcaaaatcaa atggatactt ttctaatgtc
    13201 tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat gacttcaaaa
    13261 tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg ataaacatac
    13321 tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct gccccaatac
    13381 gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag actgatcaat
    13441 tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat aacagtcatt
    13501 gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa acatttctat
    13561 ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt aaaacgggaa
    13621 ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat gattccagct
    13681 gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat actatcggca
    13741 atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat agcttgtaca
    13801 gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt tgcactccgt
    13861 acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac aaccgtagct
    13921 ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg agataagata
    13981 atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag accaaatgga
    14041 tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg gatattagct
    14101 tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg tttctcaaca
    14161 aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc tagtaatact
    14221 ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt gacagccatt
    14281 ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa aagtgtgttt
    14341 atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt tataaaatat
    14401 agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca tatgcttctg
    14461 tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct tctagtagtt
    14521 tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg ttgacatcga
    14581 aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac acattttctg
    14641 taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc agaatcttca
    14701 caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt ccttgaagtt
    14761 ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg aataggacgt
    14821 gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca attggcaata
    14881 tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta gaagtgatct
    14941 tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt gcctcactat
    15001 ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac tctaaagctt
    15061 ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct agagtttcat
    15121 ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt ttcttttggt
    15181 cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt tgttccaaga
    15241 ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca gcggtatttt
    15301 gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat tgtttctgtt
    15361 cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt tttttagcac
    15421 ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt tctactaaat
    15481 cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat tttgttgctt
    15541 cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt tctgttttac
    15601 cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt tgcatttttt
    15661 catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat ttttttgctg
    15721 cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt gtttgtttta
    15781 attcatacat atctactcac ctcttacagt tcttctacga taatattatg attagtaaag
    15841 atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc actcatgtcg
    15901 ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc tcctgatccg
    15961 atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac aagaagcatt
    16021 tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg ctgtgtccgc
    16081 cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc gtttagtttg
    16141 ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa acctacaaca
    16201 acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac aacagattca
    16261 cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc gacggcacag
    16321 atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat ttgttctttt
    16381 ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact ttcttttgtt
    16441 acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc ttggactgtc
    16501 cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag catgtgagga
    16561 tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata ttcaattcca
    16621 gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc ttcgtgatat
    16681 tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc ttgagcaaaa
    16741 gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat gacctctgaa
    16801 tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat gcctgacccg
    16861 taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc tgctacgctg
    16921 tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa atttttcttt
    16981 ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag aagatactga
    17041 taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata gctaggacga
    17101 tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt cagcagatca
    17161 gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc atatgcttct
    17221 ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt ttgttcccac
    17281 gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag cacagcgttt
    17341 ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt tcacaattgg
    17401 cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa aagagcgaga
    17461 ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca gttttttcta
    17521 attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct tcatatcttg
    17581 cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg gcattcatcg
    17641 gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca atagctgttt
    17701 cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg attccagcaa
    17761 gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga cctgcaaaga
    17821 acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc gaattcataa
    17881 agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac ccaggaatca
    17941 tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc acgatgttgt
    18001 ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga cgtttgccag
    18061 ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt ttgaatcctc
    18121 gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc tcaaaagatt
    18181 taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat tcctcttctg
    18241 tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc aaatatactt
    18301 tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag aaataaaaac
    18361 catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc aaaggtcctg
    18421 ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc actgttacta
    18481 atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg tcaacagcta
    18541 aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc gaattcaatc
    18601 ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct cttaatgaat
    18661 tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat ttgactggac
    18721 gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca gcttcacttc
    18781 cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct tttctattat
    18841 ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc gcctttgact
    18901 ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg tttatcccaa
    18961 gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt tttcttagat
    19021 tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat ttcttttacg
    19081 attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt cccatatttc
    19141 cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc tggttcatct
    19201 ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc aaacggttta
    19261 tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt atccaaatct
    19321 ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc acagattaat
    19381 gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa ttttacatag
    19441 tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc taccccgttt
    19501 tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg agtaaaatgt
    19561 tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc tggaagaata
    19621 ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat gaatcctttg
    19681 aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa tgtcactttc
    19741 attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca gatcaatgta
    19801 taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt acgaagcaca
    19861 ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt gatctttcgg
    19921 tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc ggcctctgct
    19981 tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa gccgactgtt
    20041 ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat ggtttttctt
    20101 gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt gaaaggattc
    20161 gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc tgttccatca
    20221 attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt cttaccgtcg
    20281 attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc gtcgatgctc
    20341 caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat aatttttaaa
    20401 gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca gagaatagga
    20461 ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc atctactaaa
    20521 tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt tgtgatttca
    20581 ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc taaatgccaa
    20641 gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg agcttttttt
    20701 gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat ataatggggt
    20761 tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct tatatggcca
    20821 acactggcca ttactttata gtttcgtcca agatatttct caattgtctt ggcttttgca
    20881 ggtgattcta caatcactaa atatttatac gccattactg aatggactcc tttccattgt
    20941 cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat ccattcagaa
    21001 aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt ctgccaagat
    21061 atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac atccggtaga
    21121 atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat ccaatgcttg
    21181 ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg ttcctttaga
    21241 caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc cggattgagg
    21301 aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt cacgattttc
    21361 agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc caccatgaga
    21421 gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga cgatcacata
    21481 tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta aagcagaagt
    21541 gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat caccattata
    21601 aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt tgggatcaaa
    21661 gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa tttcctgcca
    21721 acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga tgtcttcaaa
    21781 tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt ttccttgact
    21841 actgattcct tttgtataag caagtttcaa taaattttct tctatttgat acatcataaa
    21901 accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt tattgtaaat
    21961 atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt cgttcaagtc
    22021 cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat ccaggataaa
    22081 gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata cttgcagcag
    22141 caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata ggcaaatcca
    22201 aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct actgcttgga
    22261 tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc tgatcaacga
    22321 tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt tctcgctttt
    22381 tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc ggcaagataa
    22441 ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca atgccggcaa
    22501 tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct tcatattttt
    22561 ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct tgctgcacgc
    22621 ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc tgtatgcttt
    22681 tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc catttcttcg
    22741 caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac gactaattcg
    22801 ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat cagtaattcc
    22861 ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat tcttcctgaa
    22921 taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg gacaagctgg
    22981 tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc aaatttgggc
    23041 cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag ccactgctga
    23101 ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac tagtcgattc
    23161 atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat agcgcgaggc
    23221 ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt agccgcaggt
    23281 actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag atggccttgt
    23341 ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc cgctttattc
    23401 aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt acgagaagat
    23461 aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat tttttcagaa
    23521 acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat tgttctctcc
    23581 attcctttca taccgg
//
LOCUS       DAZUMO010000038        23459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000038 DAZUMO010000000
VERSION     DAZUMO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.038"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1743
                     /locus_tag="ACG8ER_001601"
     CDS             82..1743
                     /locus_tag="ACG8ER_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5050183.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1813..2160)
                     /locus_tag="ACG8ER_001602"
     CDS             complement(1813..2160)
                     /locus_tag="ACG8ER_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS5050184.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2244..3185
                     /gene="opp3b"
                     /locus_tag="ACG8ER_001603"
     CDS             2244..3185
                     /gene="opp3b"
                     /locus_tag="ACG8ER_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5050185.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3186..4229
                     /locus_tag="ACG8ER_001604"
     CDS             3186..4229
                     /locus_tag="ACG8ER_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050186.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4235..5305
                     /locus_tag="ACG8ER_001605"
     CDS             4235..5305
                     /locus_tag="ACG8ER_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050187.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5295..6248
                     /locus_tag="ACG8ER_001606"
     CDS             5295..6248
                     /locus_tag="ACG8ER_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050188.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6260..7270
                     /locus_tag="ACG8ER_001607"
     CDS             6260..7270
                     /locus_tag="ACG8ER_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5050189.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7555..8376)
                     /locus_tag="ACG8ER_001608"
     CDS             complement(7555..8376)
                     /locus_tag="ACG8ER_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5050190.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8473..8751)
                     /locus_tag="ACG8ER_001609"
     CDS             complement(8473..8751)
                     /locus_tag="ACG8ER_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050191.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9019..11028
                     /gene="metG"
                     /locus_tag="ACG8ER_001610"
     CDS             9019..11028
                     /gene="metG"
                     /locus_tag="ACG8ER_001610"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS5050192.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11507..12094)
                     /locus_tag="ACG8ER_001611"
     CDS             complement(11507..12094)
                     /locus_tag="ACG8ER_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050193.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12269..13591
                     /locus_tag="ACG8ER_001612"
     CDS             12269..13591
                     /locus_tag="ACG8ER_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5050194.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13746..14522
                     /locus_tag="ACG8ER_001613"
     CDS             13746..14522
                     /locus_tag="ACG8ER_001613"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5050195.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14515..15084
                     /gene="rnmV"
                     /locus_tag="ACG8ER_001614"
     CDS             14515..15084
                     /gene="rnmV"
                     /locus_tag="ACG8ER_001614"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS5050196.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15077..15961
                     /gene="rsmA"
                     /locus_tag="ACG8ER_001615"
     CDS             15077..15961
                     /gene="rsmA"
                     /locus_tag="ACG8ER_001615"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS5050197.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16412..17041
                     /locus_tag="ACG8ER_001616"
     CDS             16412..17041
                     /locus_tag="ACG8ER_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050198.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17165..18274)
                     /locus_tag="ACG8ER_001617"
     CDS             complement(17165..18274)
                     /locus_tag="ACG8ER_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050199.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18483..18905
                     /gene="mgsA"
                     /locus_tag="ACG8ER_001618"
     CDS             18483..18905
                     /gene="mgsA"
                     /locus_tag="ACG8ER_001618"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS5050200.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19092..19190
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19228..19764
                     /locus_tag="ACG8ER_001619"
     CDS             19228..19764
                     /locus_tag="ACG8ER_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5050201.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19804..21567)
                     /locus_tag="ACG8ER_001620"
     CDS             complement(19804..21567)
                     /locus_tag="ACG8ER_001620"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050202.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21570..23285)
                     /locus_tag="ACG8ER_001621"
     CDS             complement(21570..23285)
                     /locus_tag="ACG8ER_001621"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050203.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7562 a         4294 c         4771 g         6832 t
ORIGIN      
        1 tttaataatt attcattaaa ctcttaaaca acttcagaaa atattaatta ttctgaaaat
       61 tccaataaag gggtacgtaa aatgaaaaag aaatttacat ttggtttggt tgcggtatgc
      121 ggccttgtac ttgctggatg ttatggcggc agttcagcca caagcagtag ttcagcaaaa
      181 agcggttcag cagatggagg aggcgtattt aacttagtcg tgcctcaaga aatgcctaca
      241 gctgacttat cagttgcaac agatacaatc agttttacag cattgaataa cgtttatgaa
      301 ggaatttacc gtcttgatga agacagcaaa ccacagccgg caggagctag cgagttggca
      361 gaagtcagtg aagacggatt aacttacaaa atcaaattac gagaagacgc aaaatggtca
      421 aatggtgatc ctgttacagc agcggattac gtttatggct ggcaacgaac agtagaccca
      481 gctacagctt ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga agacattaca
      541 gctggaaaga aagataaatc agaactaggg atcaaagcag ttggtgacta tgaattagaa
      601 atcaaattga cgaaacaaac accatatttc caatatctgc ttgctttccc atcatttttc
      661 ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct atgcatctgc aagtgataaa
      721 gctgtttata atggaccatt cacattagaa gattttgatg gaccaggaac agatacagaa
      781 tggacttata agaaaaatga tcattactgg gacaaagata cagtaaaact ttctgaaatc
      841 aaagtcagtg tagtaaaaga atcttctact gcattgaact tattcaaaga tggccaagca
      901 gatgacgtga tcttgtctgg tgagttagca cagcaaaacg caaacgatcc agcttatact
      961 tctgttaaag aagcgcggac aagctatatc gaattcaatc aacgtgaaaa gaattcacca
     1021 ttcaacaatg tgaacctaag aaaagctatt tcttattcta tcgatcgtga agctttagta
     1081 aaacaagtac taggtgacgg atcagttgtc tctacaggat tgatcccatc agatatggta
     1141 aaaagtccaa caacgaacga agactttgca aaagaagctg gaaaattagt ttcttatgac
     1201 aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt tgattcttta
     1261 gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga atatgttcaa
     1321 aactcaatcc aagaaaactt ggatggcgta acagtcaaac caacacctgt tccattctct
     1381 gtacgtcttg accgctcaac ttcgggtgat tttgacgcag tactaggcgg atgggctgct
     1441 gactatgcag acccaagcag ctttactgat ttgtttgtca caggtaattc atacaaccgt
     1501 ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag cagcttcatc aacagacgct
     1561 ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa tcaagatcat ctctgatgat
     1621 atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga aaaaatcaaa
     1681 ggtatcgttc accatgcggc aggcgcttca tgggattaca aatggacata cgttgaagaa
     1741 taatcgttaa taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc gcaacctcgt
     1801 tctcatattc acttatccta acaaagaaaa aagcatgaaa atagcagaaa gagcaattag
     1861 tattccggca atgatcagcc aaaagctgta cataccgtag ctggcagacg agagcgagga
     1921 aaattctggt tttttctttc ggttccttgt tcgtgcaaga tggaccgatc gctgaaaatg
     1981 atcgaagaaa cccgaagaaa agacccataa aaaaccgccg ataatcagaa acggcaatga
     2041 ccataagaat agatcattag ataattgaat gagtgtaaag gaatggttga tgagattttt
     2101 tattaaaata accagaatca gtagaaaagc tactgcgtag gggatagatt tttttttcat
     2161 ttgattgctc ctttattttt ggttcattat ctaataatgt agcgaaacat ctgtcagaag
     2221 tcaaaagcta gggggaaaca ttagtgaata gttatatcaa atatgtatta aaacgtgtct
     2281 ttttcatgat catcacgctt tggttaattg caacaatcac ctttttcttg atgcaattgc
     2341 tgccgggcac gccatatacg aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa
     2401 ataaacaagt cggtttggat aaaccagtta tcgtacaata tgggatttat ctttctaatt
     2461 tgcttcaagg agattttgga atttccttcc aatttaaaaa ccagccagtc gcacatttgt
     2521 tagctggaag aatcggacca tccttgcagt tagggttaca ggcaattatc tttggaacag
     2581 tcctagggac gattttagga acgatctctg ccatgaagca aaatacatgg gcagatacat
     2641 cttctacact cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt gctgttttat
     2701 tacaatatat ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg gatggctttg
     2761 tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt
     2821 ttatccgtac ggaaatggtg gaagtactgc atagtgatta tgtggaatta gcaaaagcaa
     2881 aaggattgag ccgttgggaa atcgccttta aacatggtct tcgaaatagt ttgattccat
     2941 tgatgacttt attaggaccg ttagctgtag cattgatgac cggatcactg gtggtagaaa
     3001 atatctttgc tattcctggt atcggggaac aattcgttaa gtcaatcaca acaaatgatt
     3061 atcctacgat catggctgta acgatccttt actcatttat gctgattttt gttattttga
     3121 tcgtggatct attgtacggt ctagttgatc caagaattcg tgtttcagaa gggagccgag
     3181 gataaatgga aatgatacca aagcgttatg aaacagttgc agatatccct gcggatgaat
     3241 ttcttccatt acaaaaaaat accgaagaag aacgtgaaca gattactgca ccttctttga
     3301 attttatcca agattcatgg cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg
     3361 ttttactatt ggtcatcatt ttgatctctg tgatcacaat ctttgtttct cctcatgatc
     3421 caactgccca aaacgtggct tatatcaatt tgcctcctcg tattccggga atcaatatcg
     3481 atggattgaa tggaaaagca atggttggtg gtgaacttgt tgataaatat gctcaagcaa
     3541 acgtcccttc aaacgtaaat ttctttttag gaacagacgg tttgggacgt gatgtattaa
     3601 gccgcttatt tatgggaatg agaatctcgt tgctgatcgc atttattgct gcattactag
     3661 atgtaacgat tggtgtagct tacgggttgg tttctggttt gttaggcgga cgtgtcgata
     3721 acgtgatgca acgttttttg gaaatcttat caggaattcc aaacctcgtt gtcatgattt
     3781 tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg gccatcacaa
     3841 actggatacc aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat
     3901 atgttttagc tggattgaca ttaggtgaat caagaatgaa aattgcattc aaacatattt
     3961 tgccgaatat ctctagtgtc attattattc aaatgatgtt tagtattcca tctgctattt
     4021 tcttcgaagc ctttttaagc tttatcggtt taggtcttac gcctccgtca gcttcattag
     4081 gaacgatgtt gagcgaaggg tacaagacat tcctgtatct gccttatcta ttatggattc
     4141 cggcagtaac tttatcggtt atcatgattg gctttaactt acttgctgat ggattgcgtg
     4201 atgcctttga tcctaaaatg aaagagtgaa gacgatgacg aaaattttag aagtgaaaga
     4261 cttagaaatc tcttttgata cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt
     4321 tcatttgaat aaaggagaaa cattggcgat cgttggggaa tctggaagtg gaaaatcagt
     4381 gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat
     4441 cctctttaaa ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg
     4501 taaagaaata gcgatgattt tccaagatcc aatgacctct ttagatccga cgatgcccat
     4561 tgggaagcaa gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa aagagggaca
     4621 aaaagctgca ttagaattgc ttaaattagt tggaataccc gaagctgaga aacgtatcaa
     4681 cagttatccg catcagttct caggaggtca gcgtcaacgg atcgtgatcg cgattgcact
     4741 ggtatgttat ccggaaatcc tgattgctga tgaacctaca acagcgcttg atgtgactat
     4801 tcaagcacag attttggagc tgctgaaaga aattcagaca aaaatcgata catcaattat
     4861 ctttatcact catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta
     4921 tggaggaaga atcgttgaag ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc
     4981 ctatacttgg ggactgcttg gttcaatgcc gacattagat agtgcgaacg atcgattgta
     5041 tgctattcct ggttctccac cggatttatt gaacccgcca aaaggagacg cattctatcc
     5101 aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt ttgaactatc
     5161 aaaaacacac aaggcagcaa cttggctact tgctccgcag gcacccaaag tgacaccgcc
     5221 agaagaaatc caacgtcgct gggctatctt taaagagaaa caaaaacata cttacggtca
     5281 aggaggaatt gctgatgcca aataaactac ttgaagttaa aggattgaaa cagtatttca
     5341 atgtaggaaa aaagaatgaa gtacatgctg taaatgacat cagctttcac atttatgaag
     5401 gagaaacatt cggtttagtt ggagaatctg gaagcggaaa atccactact ggccgtacga
     5461 tcattcgctt gaatgaacct actgatggtg agatattatt cgacggtcaa gatataacga
     5521 agatcaaaga taaaaaaggt ttgacgaaat tccggcatga tgttcagatg atctttcaag
     5581 atccatacgc ttcgttgaat cctcgtatga aagtcagaga tatcattgcc gaaggaattg
     5641 atgttaatgg actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat gatctattga
     5701 gaactgttgg attgaatcca agccacggta ctcgttatcc tcatgaattt tcaggtgggc
     5761 aacgccagcg tattggtatt gcacgagcat tggcagtaaa accgcgtttt atcatttgtg
     5821 atgaaccaat ttctgcacta gatgtgtcga tccaagccca agtggtcaat ttgttgcaag
     5881 atctccaaaa agaacatcaa ttgacctatc tatttattgc acatgatttg tcgatggtta
     5941 aacatattag tgaccgaatc ggtgtaatgc ataacggctt gttattagag atgggaacaa
     6001 gtgatgaaat ctataatcac ggtgttcatc cttatacaga aagtttgtta tctgctattc
     6061 ctctaccaga tccagaccat gagagaaaac gcagaagaat caaatatcaa cctgaaccag
     6121 atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg
     6181 agcaggaagt cccatattac gcaaaaaaac taaaacgaca aaaggaatct cttctggttg
     6241 ctaactagga gggacataga tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga
     6301 acaaaaacag caattgattc gtacttttgg atgtgctcga tttacctata accatttgct
     6361 gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc tcacacccgc
     6421 tacattaaaa aaagaatacc cattcttaaa agaagtagac agtcttgctt tagcaaatgc
     6481 acagcttaat ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa
     6541 gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat
     6601 ctatctggaa aatggctact taaagttacc aaaacaaaaa gaactaatca agataaatca
     6661 gcatcggcca gttgaaggat cgattcgctc cgctacgatt tctgctcgat ataatgaaga
     6721 attttatgtc gcactacttt gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg
     6781 gatcgggatc gcctatcatc cgaagacatt gatccaaaca tctcgacctc tagaagtaac
     6841 tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat gcacagcgga aattgaatgt
     6901 gaaagtccgc tccgctcacc atagaaagat caggttagat caggcaagca attatcagaa
     6961 acaaaagaga aaagtaatgg atctttatct gaaacaaaag aaccagcgag aagattatct
     7021 tgaacaatta tctgggaaat tggtcaaaca atatgactac ttatttgtag aatcttttcc
     7081 aaaagaagag gcacatgctg atttttctat tcacgactgg cataaattaa taacaaaatt
     7141 gcgttataag tcccaatggt ataataaaaa atttcttttg atcaataccg acggagcaga
     7201 agaaagcaat tccgtaagaa agagccaagt agtagaaaaa atgggcagac attcggttat
     7261 caagggataa aaaatgaatg aaactgatag gagcgttagg gttagccttg ttaataaaaa
     7321 gtcttaatga cttttgtttc taggaatttt tcttttgaag aaaagctcac taattatgac
     7381 aaaccaataa aacaatagca gaaaaagttc gtacgagctt tttctgctat tgttaatttt
     7441 ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga
     7501 gttgtttttg cgttttttct tgaatgatta ggtctgaatc ttttgctcac aaccttattc
     7561 atggtgcccc ggcttgagct gatagatttt tgaaggacgg ccgattccta caggacgttc
     7621 accgatctca tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt
     7681 ataatcgatc cctaagaatt tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt
     7741 acctaaaact tgtagaactt gcggctcgtg ttccatttta ttgactacac ggttgaatgc
     7801 tttcaagatg atttgactat ggtcaaaagc caaagtatct ttgccagaag actctccagt
     7861 gactaaatca agcgagatag ccacttctcc gctagataaa tggatcttgt tactatgacg
     7921 ttctagtgtg aaccagcgta cttccttggc atcatctccg gcgattagcg gttcttctcc
     7981 aataaaggct aagtagctga ctgtgacgac ccagcctcgc ggatctcgat taggcgtact
     8041 gaatgtatgc agttgctcaa tattttcttt tgaaataacc acaccagttt cttcctttgt
     8101 ttctcttaag acgctttctc cagttgactc atccttttgg acaaagccac caggcaatgc
     8161 ccatgaattt cgataaggat gcccttttct ttggatcaac aaaactttca gctgatcttc
     8221 ttctttgtta tagcacatca gtacgatatc gacagtcaaa gagggcttgt catattctgg
     8281 acgttcctgt ttttgatacc agttaagaaa ttcttcttca cttgctttat gttcataata
     8341 taatttttct tcttctttag aagcaaaacg gttcatctga ccacctccat tgacagtaaa
     8401 agtacttttt accttattgt agcagactct gaaaaaaaac caaggattta cttgaaaaat
     8461 ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata aaaagaaaag
     8521 cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc cacaaataac
     8581 gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg
     8641 cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata ttaaatgtgc
     8701 taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc
     8761 agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga aataaaaagc
     8821 cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct attcatcctt
     8881 ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca ttttgaaaaa
     8941 aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg catcatgcga
     9001 taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac gccaatctat
     9061 tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg tgacgcaatt
     9121 gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt ggatgaacat
     9181 ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat
     9241 aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta tgataaattc
     9301 attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga tcgtttatta
     9361 gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc tgacgaagaa
     9421 ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt
     9481 ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata cttcttccgc
     9541 atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga attcatccag
     9601 ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt agaggatctt
     9661 gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc gaaacatgtg
     9721 gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata tggctccgaa
     9781 gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg taaagaaatc
     9841 gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt gcctctacct
     9901 aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa
     9961 ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc actccgttat
    10021 tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga agattttgtt
    10081 tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg aacgattgct
    10141 atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt cactccattt
    10201 gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga tgcaatggaa
    10261 aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg tgcaaacaaa
    10321 tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa agctgaattg
    10381 aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt acttcagcca
    10441 atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc tgaaacaatg
    10501 aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt
    10561 acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca agaaaaaatg
    10621 tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga aacagaactt
    10681 gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga actaaaagta
    10741 gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca attccgacta
    10801 gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat
    10861 ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg
    10921 cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg ctctttacaa
    10981 gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa attcaaaggg
    11041 ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca attttcgtaa
    11101 attccttctt attacttgaa gccaatcata taatacagaa acagagaact atccaaaata
    11161 aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat aatactaata
    11221 ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt ttaattgatt
    11281 catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa tgactttatt
    11341 acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc tatcacttta
    11401 tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa aaacaatcat
    11461 cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac ttgattttat
    11521 ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg
    11581 ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga tagagctttg
    11641 tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata aaccaatgat
    11701 tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa taaaaataga
    11761 tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat aaaaagaaaa
    11821 tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa tacaacatcc
    11881 cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac aaagtcagca
    11941 atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt aatgtgaacc
    12001 aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg gataaataat
    12061 ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc gtggaaaagg
    12121 taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg agtttttcac
    12181 tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg aaaattatac
    12241 caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct
    12301 gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac
    12361 tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt ggcttttggg
    12421 tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag caatcccgga
    12481 aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca gctttttatt
    12541 aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat cgattatctt
    12601 tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt tgattgcagt
    12661 gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt
    12721 acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca tatggggatt
    12781 attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa aagatgttac
    12841 ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg acggctggtt
    12901 aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc ctaaagcaat
    12961 tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg tttttttgaa
    13021 aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag catcagacgt
    13081 catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct cggtatacgg
    13141 tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg ctttagaaga
    13201 cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg ttccttatgt
    13261 tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt ctttcgactt
    13321 tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt ttatcggtgt
    13381 ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac cactttatcc
    13441 aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga cgatgatgac
    13501 gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc cagtctatta
    13561 tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca gaaaaagtac
    13621 gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt tcttctattt
    13681 tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga aaaggagaga
    13741 taaacatgat atttgattct catacccatt taaatgctga acagttcaat gaagatattc
    13801 cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg
    13861 atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat atatacagta
    13921 ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa aataaattac
    13981 aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg gattatcatt
    14041 ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt gctatcgcaa
    14101 aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac acgtatcaaa
    14161 tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc agtggtgatt
    14221 atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc ggtgtcgtca
    14281 ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac
    14341 ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag cgaaatgaac
    14401 caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt ccgacagaag
    14461 aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc gaacatggat
    14521 aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg
    14581 gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga agagatcctg
    14641 atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga tcctgatttt
    14701 tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa acatgctttt
    14761 ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt
    14821 gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc ccaaacggct
    14881 gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg ggcacaagca
    14941 aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa
    15001 ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca agcaatgaaa
    15061 atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga
    15121 gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt ttctgactga
    15181 accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat
    15241 cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg caaaacaagt
    15301 cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga tgcaacccta
    15361 tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa cagctgttag
    15421 agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt actatattac
    15481 gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac tagtcgtcat
    15541 gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag cttatggttc
    15601 attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg taccaaaaac
    15661 agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa gaagagatat
    15721 accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag cggcatttca
    15781 gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag atgaccagac
    15841 aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac gtcgtgggga
    15901 gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta
    15961 aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta ataagaacag
    16021 ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg cagatgcaag
    16081 cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta agtcacaagg
    16141 aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt acttgatgca
    16201 agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt cacatctcct
    16261 atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt ttgtatggat
    16321 ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa gtcgacattt
    16381 atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc gaaaactaac
    16441 gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc ctaaaatcat
    16501 gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg cgatgttttt
    16561 tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct ttttaacaac
    16621 gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga ttggggcttg
    16681 gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt ttgctaattg
    16741 gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag aaatgctagt
    16801 agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc aaggatattt
    16861 ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag ttgattataa
    16921 tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg tatttacggc
    16981 agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa cgacatatta
    17041 gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc ctctaagatg
    17101 agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc taacaggggg
    17161 caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg cctttgctga
    17221 tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca tctacttttg
    17281 agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg
    17341 aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct acttgttcac
    17401 tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa cctttttcaa
    17461 ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca tctgtgataa
    17521 caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata aagccggcaa
    17581 cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt tggataaatc
    17641 cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa
    17701 catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca cgcatggcta
    17761 cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact tttgcatccc
    17821 gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa gcagctggtt
    17881 ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca cgttttttga
    17941 tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca aaaacagtca
    18001 tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct ttaggtgcca
    18061 cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct tccagtcccg
    18121 cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa acgataaatt
    18181 cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca ttgtcatatt
    18241 ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt tcttttttag
    18301 ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt catgttgaac
    18361 aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa tatccataag
    18421 aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc
    18481 ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa
    18541 cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg actgggcaga
    18601 ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg ttaggcgggg
    18661 atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt tttcttcgag
    18721 acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt ttatctgatg
    18781 tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta agaggactgg
    18841 aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat ccactttcat
    18901 tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt agctataaaa
    18961 gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat aaacaataat
    19021 attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt gggaaaagat
    19081 cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg ggatgttgcc
    19141 tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca tgatacgatg
    19201 tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc acgtatgatt
    19261 gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt agcaatcgag
    19321 accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg cattcttttt
    19381 ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat ggccctcttc
    19441 tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg agcaatctac
    19501 ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc tacgcttagt
    19561 gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat gtaccatgtg
    19621 ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat atggttaccg
    19681 attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa aattttaaga
    19741 agtttagcga tccacgctaa ataaaaaaca agcagtcttc ccatcccaga agactgcttg
    19801 tttttattgt gctgtgaatt gtgcttgata gagccgtcgg taataaccat ctttttgaag
    19861 cagctcatca tgggtgccca tttcgacaat agctccctga tccatcacta atatctggtc
    19921 cgcatttttg attgtggaca ggcgatgggc aatcacaaaa ctggtttttc cttccatcat
    19981 tgctaagaag gcttgttgga tttttttctc tgtcaatgta tcgactgagc tggtagcttc
    20041 atctaagatc aacatagggg gattactgat catcgttcga gcaatcgtca acagttggcg
    20101 ttgtccatct gatatcttta acccttgacc gccaatctta gtatctaatt tttccggcaa
    20161 acgttctaca aattcataca tataagaggc ttttagggca tcattgattt ctgcgtcact
    20221 tgcttccgga ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata gccaagtatc
    20281 ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg
    20341 gtcatcgact gtaattgttc cttttgttgt gtcataaaaa cgcatcagca aattaacaag
    20401 tgtggatttt ccggcccctg tttttcctac tatagcaatg gtttgacctg gataagcagt
    20461 aaaattgaaa tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa
    20521 agagactttt cctttgatat ttttgggttc caaggcatag gcatctggca aatcaggctt
    20581 ttcttctggc tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag cggtttgcag
    20641 ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa aactgacttg agtagatcgt
    20701 aaagctggaa atcacaccaa cagtgattga cgaaccactc ttcaatgcca ataggccgcc
    20761 aactaaacca atcgataaat aagcaaggtg atctacaaaa cgagaagacg ggtttgtcaa
    20821 ggaagaagaa aattgtgccc gttgtccttt ttcatataga tcagcattta gcagttcaaa
    20881 ttgctgttga ctagcttctt cttgctgaaa agctttcacg attttctggt taccgattcg
    20941 ttcggtcaca aagcccgaga tatccccaat aatcctttgc tgtctatcga aatttgtttg
    21001 tgaagcacga gcaacgatcc aactcacaag gaaaataatt ggtgtagaac aaagaacgac
    21061 caatgtcaga agtggactta gacgcatcat aaaaataaac gaaagaatga tgattgccac
    21121 tccagaaaat aattgattga aggccgcaga aattgcaatg gaaatattgt ccatatcatt
    21181 tgtaaatcga ctgacgatat ttccgtggga gttttgatca taataacgaa gtggcaatcg
    21241 attcagatga gcaaatgcat cttttcttaa ttttgcaacg gataagtaag ctaaccgatt
    21301 gcttaacact tggatgagcc attgactgac tacgttgatt aaaacgatac caccgaatag
    21361 aataagtaaa tggaacagtc gattgaagcg gacctgtcct tctccaatca tttgatcgat
    21421 actttgacca atttgtaacg tcatcacaac tgttgcaatc ccattgatga ttcctagtat
    21481 cacagctagc caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc ggcggagcgt
    21541 ctgcaaggat aattttttta ctttcataac tattcttcct cctgtgatgc gacgatttcc
    21601 tgatattctg ctgattgagc aagtaattct tcatgggtac cctgagcagc aatcttacct
    21661 tgatccatca ctaagatatg atgtgcttct tgaatagagc ggacacgttg ggagataata
    21721 atcactgtac tgccaagata agtctgtagc gccttacgca aatccaagtc tgtttgataa
    21781 tccaacgcac ttaaagagtc atctaaaatc agtaatgctg gcttggcaat caaagctctt
    21841 gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt tcccattttc catgactttt
    21901 gtatctaaac catttggaag ctgacggaca aattcttctg cctgagcgat tttcagagct
    21961 tcccaacatt ctttttctgt ggcgtctttc ttaccccatt gaaggttttc acgaatagtc
    22021 ccggtaaata gtacagaagt ttggggaaca agagcaattt gctgacggag atgatccagc
    22081 tgccaagaac gaacatccat tccgtccatt ataactgtac cggaactgac atcatagaaa
    22141 cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat gattccaagg
    22201 actgtatttc gcttcagctg aaatgtaata ttttggagag aaagaccaga atcaggagta
    22261 taacggaagt caacatggtc aaaagcgata attgcttcag aagaaaaatc gggagtagta
    22321 ctgacctcag cttcaagaat cgaattttct gtgtctagca cctctttcac ccgatttcct
    22381 gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag tgccaaaagc
    22441 atttgattca tataattgat caaagcaaga acttgtcctt gttgcaagct accaatattt
    22501 actttgatcc cgccaactgt cagcaaacag atgatcccga cattcatgat caaggttgta
    22561 gcaggtgata atagggcaga gatattggaa acacgtaaat aattgttcgc tagatcattt
    22621 gtgcttttat cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg gatgacacga
    22681 acaccgctaa gattctgact aacgtgttca ttgagctgat ctagtttttc ttgcactttt
    22741 tggtagagag gaacggtttt tttgatgata aaaaataaaa tgattgagaa aataggcaat
    22801 aatgctaaga aaaacagacc tacttcccag tcgatcacaa aagccatgac cactgacccg
    22861 atacttaaaa acggtgctct gatcaataaa cgaatgacca ttgctaaagc tagctgaagc
    22921 tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg attcaactcg
    22981 ttgtgagaga aagtattgat ttttttgatt agctgattac gtaactctgt accaaatcct
    23041 tgtgaagcta ctgaagcata gtattggcaa atgatagcac aaatcagacc aatcacagac
    23101 atagcgacca tccataagcc catttcaatg atttttcctc ggtcattcat ttttagtcca
    23161 ttatcgatca aggaagccat cattaaaggc aggacaagtt cgaagacagc ttcaagaaat
    23221 ttgaagaacg ggcccaaaat gatttgctta cgatagtctt ttgcataacg taataactga
    23281 atcatatgat tcctcctatt atttatttgt ccacaattga aaaaacaagt actaccacat
    23341 tgtagcttag gaaatttaga atgaaaagag aacaagcctt ttcataaatg aaagttttat
    23401 tatgaagaag ctttagaaat tgaatagact atttttttta aaaaaaagaa aatgagaaa
//
LOCUS       DAZUMO010000039        23375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000039 DAZUMO010000000
VERSION     DAZUMO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.039"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..1384)
                     /gene="queG"
                     /locus_tag="ACG8ER_001622"
     CDS             complement(233..1384)
                     /gene="queG"
                     /locus_tag="ACG8ER_001622"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS5050204.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(1403..2155)
                     /locus_tag="ACG8ER_001623"
     CDS             complement(1403..2155)
                     /locus_tag="ACG8ER_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050205.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(2296..3246)
                     /locus_tag="ACG8ER_001624"
     CDS             complement(2296..3246)
                     /locus_tag="ACG8ER_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5050206.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(3247..4086)
                     /locus_tag="ACG8ER_001625"
     CDS             complement(3247..4086)
                     /locus_tag="ACG8ER_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5050207.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(4083..4829)
                     /locus_tag="ACG8ER_001626"
     CDS             complement(4083..4829)
                     /locus_tag="ACG8ER_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5050208.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            5067..6437
                     /locus_tag="ACG8ER_001627"
     CDS             5067..6437
                     /locus_tag="ACG8ER_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5050209.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(6492..7388)
                     /locus_tag="ACG8ER_001628"
     CDS             complement(6492..7388)
                     /locus_tag="ACG8ER_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5050210.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(7502..7672)
                     /locus_tag="ACG8ER_001629"
     CDS             complement(7502..7672)
                     /locus_tag="ACG8ER_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050211.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(7669..8346)
                     /locus_tag="ACG8ER_001630"
     CDS             complement(7669..8346)
                     /locus_tag="ACG8ER_001630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5050212.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(8437..9342)
                     /locus_tag="ACG8ER_001631"
     CDS             complement(8437..9342)
                     /locus_tag="ACG8ER_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS5050213.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            9517..10971
                     /locus_tag="ACG8ER_001632"
     CDS             9517..10971
                     /locus_tag="ACG8ER_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS5050214.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            10987..12018
                     /locus_tag="ACG8ER_001633"
     CDS             10987..12018
                     /locus_tag="ACG8ER_001633"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5050215.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            12025..13212
                     /locus_tag="ACG8ER_001634"
     CDS             12025..13212
                     /locus_tag="ACG8ER_001634"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS5050216.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            13368..14750
                     /locus_tag="ACG8ER_001635"
     CDS             13368..14750
                     /locus_tag="ACG8ER_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5050217.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            14787..15107
                     /locus_tag="ACG8ER_001636"
     CDS             14787..15107
                     /locus_tag="ACG8ER_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050218.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(15307..15975)
                     /locus_tag="ACG8ER_001637"
     CDS             complement(15307..15975)
                     /locus_tag="ACG8ER_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS5050219.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(16164..17590)
                     /locus_tag="ACG8ER_001638"
                     /pseudo
     CDS             complement(16164..17590)
                     /locus_tag="ACG8ER_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            17833..18288
                     /gene="tnpA"
                     /locus_tag="ACG8ER_001639"
     CDS             17833..18288
                     /gene="tnpA"
                     /locus_tag="ACG8ER_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS5050220.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            18553..18651
                     /locus_tag="ACG8ER_001640"
                     /pseudo
     CDS             18553..18651
                     /locus_tag="ACG8ER_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(18874..19104)
                     /locus_tag="ACG8ER_001641"
     CDS             complement(18874..19104)
                     /locus_tag="ACG8ER_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS5050221.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(19334..20152)
                     /locus_tag="ACG8ER_001642"
     CDS             complement(19334..20152)
                     /locus_tag="ACG8ER_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5050222.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            20313..21002
                     /locus_tag="ACG8ER_001643"
     CDS             20313..21002
                     /locus_tag="ACG8ER_001643"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5050223.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            21016..22518
                     /locus_tag="ACG8ER_001644"
     CDS             21016..22518
                     /locus_tag="ACG8ER_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5050224.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            22531..23007
                     /locus_tag="ACG8ER_001645"
     CDS             22531..23007
                     /locus_tag="ACG8ER_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5050225.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(23104..23214)
                     /locus_tag="ACG8ER_001646"
                     /pseudo
     CDS             complement(23104..23214)
                     /locus_tag="ACG8ER_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6997 a         4384 c         4315 g         7679 t
ORIGIN      
        1 acaaaaccat aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt
       61 tttgaatgaa aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa
      121 aaaatttttg atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt
      181 aattgttaca acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta
      241 actttctccg caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt
      301 ctttagctaa tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac
      361 gtccaatagc ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg
      421 aaatctcagg caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct
      481 gcaaaggttt cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt
      541 tattagacat cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca
      601 tctcttcatg aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat
      661 cacatccata aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg
      721 tctgagtttg ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt
      781 ttgtcggaca agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat
      841 caggctcaaa tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt
      901 cagtgatcaa caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga
      961 catcgatcag ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc
     1021 atttttctgc tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat
     1081 catggtaatc gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca
     1141 cttcttcatg gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat
     1201 caaatgtcat ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat
     1261 ggccagctgc tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg
     1321 tgaacccgat tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg
     1381 tcatggtctt cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt
     1441 tcagctggac aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc
     1501 ttgcagcaaa tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat
     1561 taaaaaaatt caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc
     1621 atggcgaaaa ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct
     1681 gtgtgtacgt gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat
     1741 aaaacatatt cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga
     1801 tctttttgat tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg
     1861 ttatttcgtc gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca
     1921 caaaataacc gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc
     1981 ctatgtacag ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag
     2041 ctaattctgc ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga
     2101 tcaatcgaaa gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca
     2161 caccttcctt ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg
     2221 aaaaacagca taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc
     2281 tagaccttgc acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat
     2341 attccatttc atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc
     2401 agtgaacagt ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct
     2461 tgagtctaca ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat
     2521 gatcgctttc atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc
     2581 tgctggcaaa tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa
     2641 ttttttgttc tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag
     2701 tttttccaca tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc
     2761 agagaatata cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc
     2821 atggggatct gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt
     2881 gctaactgca aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa
     2941 ccagctgtta cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc
     3001 ctttttgatg tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat
     3061 actgtggata tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt
     3121 ggtagccacg actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt
     3181 tccgcaaccg gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt
     3241 tttcatctat ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc
     3301 aaggataaaa aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact
     3361 ataactgaaa aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat
     3421 cattttcagt aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc
     3481 aatcaccaag attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa
     3541 catcaaaaga taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc
     3601 gaatgatgtg atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact
     3661 aacgagtgct gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat
     3721 atgatacaaa tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa
     3781 ggagctaaag acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa
     3841 accaatcact gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc
     3901 aaacatataa gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa
     3961 agacatccct cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat
     4021 ggaagtgacc aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg
     4081 gatcattttt tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga
     4141 aggttcttcg atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa
     4201 agaataacct catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc
     4261 gtttttcctg aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca
     4321 tcaatcccta cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt
     4381 gcaataaaaa ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat
     4441 tcttccatcc ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt
     4501 ctttgtccaa ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt
     4561 ggaaaggaaa gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag
     4621 tttaccgact gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa
     4681 gatttcataa atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt
     4741 ggatttatcg tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg
     4801 tcttgaatta caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact
     4861 ttttatttaa atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag
     4921 tttttttcga attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc
     4981 aataaggcta acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt
     5041 gactcaacaa cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg
     5101 cccgtcatat ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg
     5161 cctcctcaac catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat
     5221 tctttttgta tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg
     5281 gttcttttgc aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt
     5341 ggagtaatat ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt
     5401 atatgaatta ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt
     5461 ttcttatgct ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt
     5521 cgatcatcaa agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat
     5581 ttggatttgg taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat
     5641 tcttcactgg aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc
     5701 aaggaatcga actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca
     5761 ttgaagctgt ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta
     5821 tcattgtgag tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa
     5881 ctttttctaa agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag
     5941 cggcattatc aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg
     6001 ccataaatgg aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt
     6061 atcctgtcgt agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac
     6121 caatgttcat caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc
     6181 ctggtatggt tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg
     6241 cagataaaat gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga
     6301 cacttgcttt tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct
     6361 cattgattat tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa
     6421 aaagaaccgg aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt
     6481 tattgctttt atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg
     6541 ctcatcccat tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga
     6601 tttggacctt ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta
     6661 ttagtttcag aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct
     6721 accgactctg ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct
     6781 tgaagagagg cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa
     6841 ttagccgata attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc
     6901 tgatacgctg cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct
     6961 ttttggttag caactgctga taaaccagct attccctctt gatttccctg aatttctgtc
     7021 tggtattctt caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg
     7081 attgcttgaa gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct
     7141 tttgttttag ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc
     7201 gttatggtcg tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact
     7261 cctcctgcta tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga
     7321 gctgcctgtt tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca
     7381 tcatacattt ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt
     7441 cttattgtag caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat
     7501 ttcaccagtt gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc
     7561 gttcgtctat ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt
     7621 ctgatttttc gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt
     7681 tcctttcatt tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag
     7741 tgcttctacg atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg
     7801 tactctttga ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag
     7861 atactgctga taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg
     7921 tgctaaaacg aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg
     7981 cctcaaaaat tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc
     8041 cacacctgtt gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc
     8101 caagacatta tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa
     8161 ctggaaaatg atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt
     8221 tccgtctgga taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc
     8281 tttgaaaaat tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt
     8341 acgcatccct tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt
     8401 ttcttttgcc gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt
     8461 ttgtttttcc aactgttttt tcaaggtatc caaagcaata ttcttaaaat tttcgttttt
     8521 catacgttta cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa
     8581 tacgactaga ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag
     8641 agaaagaaga aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt
     8701 acccatatga tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc
     8761 gatttcctca acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac
     8821 agctaaagag gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag
     8881 gactgataaa aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc
     8941 cgtatctttc atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat
     9001 cgtgtattgg tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc
     9061 aaatgaaagc cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc
     9121 tcctggacga atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg
     9181 ataagatcct ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc
     9241 aaaagaatga aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc
     9301 tacttgttga tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc
     9361 ctttagtata atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc
     9421 gatttcttgt taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact
     9481 tagtattata tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt
     9541 gtgtcctacg ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat
     9601 tttatggtat tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa
     9661 atgattgcta cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat
     9721 cctttgattg gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg
     9781 ttagttatcg gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt
     9841 ggcttagcta ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta
     9901 ttggatagtt tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt
     9961 acggaaacaa aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt
    10021 gcaaatggta ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact
    10081 cataaacata ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt
    10141 tcaggcggta ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt
    10201 cagcaaaatg aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag
    10261 ttgatgtggc ttgcccttgc ttatatcgct tatgcgattg ccaatgtagc aacaactgcc
    10321 gtattattct acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt
    10381 ctagttgctg ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc
    10441 atctctcgtc gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc
    10501 tttacgatcg caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc
    10561 cctcaacaat tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt
    10621 caatggaaaa atggtatccg taacgaagct gttactctat caattcgccc tttactagat
    10681 aaaatcgctg gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg
    10741 actggtaatg ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat
    10801 gcattctatg ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt
    10861 acattgacag agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca
    10921 gctgatacaa tggaagatga acttcacgat gcagtagttc cttcccatta agaaaggatc
    10981 ttgaaaatga agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag
    11041 aacaaaaaca agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa
    11101 tcaaatgatc gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat
    11161 ccgtatatcg gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct
    11221 ctcaatggaa aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt
    11281 ggtgctcctg gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa
    11341 gcttctgtca tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg
    11401 gatgtggaaa tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc
    11461 accagtgacc aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga
    11521 gatgcttctc agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct
    11581 ttgcgaacag atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat
    11641 tttcaaatac ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt
    11701 gttgacggaa tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct
    11761 cgcttgacta gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg
    11821 gttattttca ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca
    11881 catcacggtg gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca
    11941 tttggttcga ttcatttaaa agccggatca gtatttgaaa cagtaacaga attcaaaatc
    12001 aaaacaagaa aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc
    12061 gaaacattcg gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt
    12121 ggtgaacaca cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg
    12181 caaggtgtag cagcacctcg agacgataat aaaatattag tttattccac aaactttgac
    12241 gatatcggtg tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg
    12301 gcaaattacg taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat
    12361 gggtttgagt tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct
    12421 tcattagaat tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc
    12481 ttggaactag tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt
    12541 atcatggatc agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca
    12601 aatacgctga aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg
    12661 aatacgaata agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc
    12721 gaagaagcct tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat
    12781 gaagaagaat ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct
    12841 cgccatgccg tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg
    12901 aacttagaag catttggtca actgttaaat gattctcacc actctcttcg ctatgattat
    12961 gaagtaactg ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc
    13021 ttaggtgcac gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa
    13081 gaaaatatac ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat
    13141 gctccggaat tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct
    13201 gttcatgttt gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct
    13261 ctcagcaatt tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga
    13321 cacggaaaga gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc
    13381 aaaatccggt atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca
    13441 aatcttttta cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat
    13501 tagtagtcat gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg
    13561 cttccagtcc tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc
    13621 ttcccttccc ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg
    13681 aaaagaacag agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg
    13741 tctattccgt cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa
    13801 caaatgcgta cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct
    13861 acacgactat aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct
    13921 caaaagacgg aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat
    13981 ggaaaatctc agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga
    14041 aacaaatggg tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa
    14101 gcaagctagg ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt
    14161 ttttgggaca agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata
    14221 gagattatac aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg
    14281 gcactcgctt gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact
    14341 ctttaggcta tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag
    14401 cgacaaatgc tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc
    14461 actaccagga tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt
    14521 tacagcttaa cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg
    14581 ttgtcgatac gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct
    14641 tgcaaggagg acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga
    14701 gtggattaga aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag
    14761 tatcgagatc ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc
    14821 aacggtatca aaaaaatgaa gaagaagcac gcaaacagcc tcttcctaaa caacaaatga
    14881 cattgcaaga aatcgatagt atactgacag aagccttttt atctcgtaaa gcagtaatca
    14941 ttcagctcaa tttaaaagat gaatttggaa gactcttgga tgaggtcgaa gggcacttca
    15001 ttggtgaagc ctatgaagat tactttatga ttgaccatca gccgatattg tgggaagacg
    15061 tacggtatat tgagttaaag gaaacaaaaa aatggtttgt gacatgagag gttgtgacag
    15121 aagtgatcaa cttcaaaaaa taagaaagga tttttcgaaa attgcttctt aaatttttgt
    15181 ggaattttgg cttatttttc gaagaagcaa cttctggaac aacgtatatt agatatcaga
    15241 ggttgtgaaa aagtcgctta gttttgagta acaagccaag gcgctgattt cacaacctct
    15301 gtctatctat ccatctattc ggtaagattc atgcatgacg tcttctttat ttagtatgta
    15361 gagccctttt tcttctgacc attcaacata aatcagctgt tttacatctg catagccttc
    15421 tttcttcaag ttctccataa gccatgcttc agtcaattga ttggactgaa gcactttatg
    15481 ctgaatttgc ccttcatcta caagcaggat cgataaagct ttgtcttcat ctgatttttt
    15541 caatacactc atttgaccgg cattctctag gataacatgt ttggcatttt caagaaaaaa
    15601 gcattcttgc tgtcttaaga ttgctcgcaa ctgctccatc tcgatatggt tatttgtcaa
    15661 ctctgtcatg ttgatgacgc cgtctttgat tagaacagat ggctcgcctt tgacccaacg
    15721 attgaccttt tcattttttt ggacgatacg ttcgatacca taaatcatta ctgcccaaag
    15781 cgcaatggca aataggacat gccctacatg gacattgtca tcataggttg attcttctaa
    15841 gatcccacct aaaacaagcg tatagactaa atcaaacggt gtgatttctg acatggattt
    15901 cttcccaagc aaacgtacca ctaataataa accgatcaac ccgaacacca gtttcattgt
    15961 aattgaaaaa atcatttttt ctccccctaa ctatctgtta attaaattat atatgctttc
    16021 tcacgaacac gcatggaata tacaaggaag aaatgtatcc ttttactaaa aaaaggactg
    16081 tgacactgta gtgaccccca aaagttagac ttttttggag tggagaaatc cactcctttt
    16141 ttttatgctc taaaatgata tttttacaca accgttacgg ttgttttacg aaattggacg
    16201 agactttgcc agtttaattt ctgttttatc cgttcattat tgtagtaata gatatagcta
    16261 tcgatggctg attttaattc ctcgaaacag ttataaactt tcccgtgaaa aatctcttgt
    16321 tttaataagc caaaaaaatt ctccataagt gaattatcta agcagttccc tttccgagac
    16381 atgctttgaa agattttgta ctccttcaat ttgtttctat aagctttcat ttgatacgcc
    16441 caaccttgat ctgagtgaaa cgtacgacga tagggacaat cgttagtggt ctggatagct
    16501 tcctccaaac cttccattac cgctaatgca ttgggctttt cagagatacg ataggataaa
    16561 atctcactat tgaacatatc cataaaagga tctagataca atttcttttg acggataatg
    16621 cctttggcat ccacttcatg gtatttaaat tcagttgtat ccgtcgtgat tttctgatgg
    16681 cagatactgg tgtagaaacg acgatggatc cgatttttgg ctactgctcc gacttgtcct
    16741 cgataagaat tgtagcgccg tgatttacgt gtgtatgtcg ttacttttat tcctagcttc
    16801 ttgatcaggc gctgaacttt ctttttattg atagagaaac cgcggtttct tagttcattg
    16861 gtcatccgta aacagccata atccttgtgc ttctcccgaa ctttaagcat ttccgcttca
    16921 atctcttgat ctggattttt acggtccaat cgtttttgcc agtacataaa gattgcctta
    16981 gggaacttta aagtttctag aagctcaact aatttaaacg gtcctcggag gcttgcgatg
    17041 attcgtgcga ttgcttcatt cgtcgttgtt gggcttcctg ttttcgcagc ttcctcaatt
    17101 cttttaaaaa ggcattttca atttgaaggg aacggactcg tttctccaac tcctcgatgc
    17161 gttcacgttc tttagacgtt gctttagttg tagatttttt cttatttttg ggttcatttt
    17221 tcttagacaa gataggtgga catcctttcg tttttgagag tccatcgatc ccaccctcac
    17281 ggaattgacg catccaacta gcgatcaaat tagggttgtt catgttaagt gtgttggcaa
    17341 cttcacgata ggacagctca cttgttagat ataactctat cgcatccagc ttaaattgaa
    17401 cagaatattt tttcttttgt cgctttctta ctagaccctc ttcaccaaat tcttgatagg
    17461 cattgatcca gcgcttcact tgtgatggag ccttaatgga atatttcttt gccagataat
    17521 cacttccgcc tttaccatcc aagtaatcat gaacgatctt taatttaagt tcaaaagtat
    17581 acttagccat aaaaaatacc gacctccaaa agttagattt tttggtctaa cttttggggg
    17641 tcggtacaaa acttaaaagt tttagaccct tttattttct taatgacttc agtcagtttt
    17701 gtgcgtaaag cgtgcgaaac aagaaaaccg cgcgtttcta cgcgtggaag aagtatcttt
    17761 agcgataaaa actcctttcg atatactagt gagtggctac caaccgctca tcagatcgaa
    17821 aggagttttt tcatgtcaaa tgacgataaa agtttagcat atactcgttg gaattgtaag
    17881 tatcatatta tttttacacc aaaatatcaa cgaaaagtaa tctatgggca attacgaaaa
    17941 gatcttggaa aaatacttag gaagttgtgc gaaatgaaag atgtggaaat catagaagca
    18001 catgcaatgc tagatcatat tcacatgctt gttcgagtac caccaaaaat atgtatatcc
    18061 agttttatgg ggtatctgaa aggtcgtagt gcggtactaa tccacgagca gcacgcaaat
    18121 ctcaaatata aatacggaaa caaatccttt tggtcgaaga gatactatgt gagtacagtc
    18181 ggattgaatc aaaagacaaa tgaaaaatac atccgtgaac aagaagctga tgatcgcatt
    18241 cgatatatta taagtaaaag agagtacgta gaaccattta aaaaataaag agaagtaagt
    18301 gagtgaagat gagcagttgg cggtctttta aaaatcccct tttaaggggc agcgagtgtc
    18361 gaccccttct agggggatta aaaatacacc cgtcctcacg ggtgtatggg ttactgatag
    18421 acttgatttt aactggtgtc agaaacttat gtccagaaag ggtgatgaaa ttttggcaat
    18481 ttacggctat tcaagaatta gcacaagcaa ccaagattac aaaacacaaa ttcaaacatt
    18541 agaaaatgct ggcgcagaaa taatcttcct agaaaatcac tggattatcg aacacctttg
    18601 gaagtatttt tgagttacgt aagtattgat gatctgtcta acttaatttg acaattaaga
    18661 gtatatttac ttcagatggt aaggcaacaa aataaaatac aataggcaga tgaactaata
    18721 aataaaatct gtattaaata cagataaatt aatataaaaa tcaaaaggat cataaacttt
    18781 taacaagtga tgaaatgggt ggaaattgat tcttatagac agtttgtctc ataacattga
    18841 agtattttgc aattttagca ttgaaacact tttttaatct tcaacgttgt tcttataaac
    18901 aagatgattg ggaatctttt gaattaacac tgtatagctt agaacaccct ttatagaaaa
    18961 cattcgctta gcaatcttaa taattccttg tgcccgatgt cgtttagatt cagtaggttc
    19021 ctgctcatcg atcatggaaa aaatttgaag gatcagatcc ttaataaatt tatttaagag
    19081 aggatgatcc attgcttcat ccatactcga taaactagtg ataatgagct acacgttttt
    19141 ctttcaagtt gatcaatagc ttccacgata aaaatatctt tagataaaat caagaaccgt
    19201 aggaactaca gggatatttg gtcaataaga gccaccgcta ttagtaaaaa ataagctatc
    19261 aagtatgctc tcatcccaag aattgcctac ttcaatcgtt aaaaaccgtt aggtttagct
    19321 aagtgggggt agttcactca atacttttat caataataga gcgcaaaact ttttctctta
    19381 attcaactga atcaatatta ttgttttgtt cataaccatg aactaataga tcacaaatat
    19441 aaagttgtga tatttttcca gctaaagatc caccattaag aaattcttca atggctgttt
    19501 gtaaaactaa gtctgcagac tttcctatag gtgaatgaat atagttagtt atagcaataa
    19561 tttttgcgcc atttttttta gctattttca atgaatcata agtatctttc gttttcccag
    19621 ataaagaaaa aatgattact aaatcattaa ctgttaataa agaagcgact tgggcttgaa
    19681 aatgagggtc tagtacagct tttgcttgaa ctcctacgcg aagaaacata ttttctaaat
    19741 caagactagt atttccacta gatcccactc caaatatata tatgtttttt gattgagtga
    19801 tcattttaat ggcttcattc aatttttctt cgtctaaaag cctaatagtg gaatagagag
    19861 cttctgttaa actattagca atttcatcat agtactttac actagtcgga tgttcttttt
    19921 tttgactaaa gtcttccttt gcaatttcaa tttttaaatc agaaaaacca gaaaaaccaa
    19981 ttttttgaca aaagcgaata atagtagcat cgccaacttt tgtgtttttc ttaatatcac
    20041 tcatcgtact ataaataata gttttcccag aatttaaaat aaaatcagcg actttttttt
    20101 cagacttcgt taaaaatgga taattttctt taattaaata gatataactc atacaaactc
    20161 ctctacaatt tcaacttttc tccattatac tccaaaataa taaaaaaaac tcctaaatac
    20221 tccattttta tctttacttt tatgaaagcg gatgctatat tgtttttgga gttgttatcc
    20281 aaaaataata aaaaattgga gtgtttagga taatggataa aaagaggttt ttagatctta
    20341 tttcaaaagg tttgattgtt tcctgtcaag cattacctgg tgaaccattt tacaaagagg
    20401 aagaaagttt aatgccttat tttgctttgg ctgctcaaag ggctggagca gttggtatac
    20461 gtgcaaattc tgttcgagat attattgaaa ttagaaagtg tgtatcatta ccaatgattg
    20521 gaattattaa aagagattat ccaccagaat ctccattcat tactgccact atgagagaag
    20581 tagatgaatt agtagaaacc ggtgttgagg taatcgctct tgattgcact cttagaaatc
    20641 gacatgatgg actatctata aattctttta ttactaaaat aaaagaaaaa tatcctcatc
    20701 agttatttat ggcagatatt tctacatttg aagaaggcaa aaatgcatat aaagcaggaa
    20761 tagattttgt aggaactact ttaagtggat atactgagga aagtaaaaaa caagatgggc
    20821 cagatataga tttgattaaa aaattggtag aagaagggat ttgtgtaatt gctgagggaa
    20881 ggattcatac tccagaacaa gcaaaagaga taaaaaaaat aggagttgca ggtattgttg
    20941 tgggtggtgc tattactcga ccacaggaaa ttgcatcaag atttgttaaa gcattaagtt
    21001 agggggagaa ataaaatgtt taaaaaattt tcacaattag gacgcgcttt catgttacca
    21061 attgctatat taccagtagc cggattacta ttgggactgg gaggagcgtt aacaaatgag
    21121 agtgctataa atgcttatcc atttttagat caaccgtggt tgcatacaat tttaagtatt
    21181 atgagttatg ctggaaatgc agtttttgca aacctagcac taatttttgc aattggtatt
    21241 gctgtaggac ttgcaaatgg agataaagga acagctggtt tagctggtgg agtagcttat
    21301 ctagtatata ctgcaactat tagtgggttt cttgaacttt tttcagcaaa agatgcaaca
    21361 ctagatacag gtgtagtggg atcaattgtt gttggtggta ctgttgcatt tttacacaat
    21421 cgttatagaa aaattgaatt accacaattc ttaggttttt ttggaggatc aagatttatt
    21481 ccgattgttt ctagtttagc tgcaatcatt attggttctt tcttttattt aatttggcca
    21541 ccaattcaaa atggtttagt agttgctggt gaacaaattg cccaaatggg tagtttagga
    21601 acttttttat atggtttttt attgcgttta acaggtgctg ttggactaca ccacaccatt
    21661 taccctttgt tctggtatac atctttaggt ggtaccgaaa ctgtagctgg atcaacaatt
    21721 gctggagccc aaaatatttt ctttgcccaa ctagctgatc caaaccacac tggtctgttt
    21781 acttatggta ctagattttt tgcaggaaga tttgcaacaa tgatgtttgg attaccagca
    21841 gcatgttatg caatgtatcg tgcaattcct aagaaaaatc gtaaaaagaa tggtgggctt
    21901 tattttagcg gggcccttac atcattttta actggaatta ctgaaccagt tgaatatatg
    21961 tttttatttg ttgctccttg gctttatgtg attcatgcat tcttagatgg attatctttt
    22021 tattttgctg atatcttaaa tattcgtgta gggaattcct tttcaggtgg tttgatcgac
    22081 tttttattat tcggtgtact tcaagggaat gacaaaacta attggttaaa attaattcct
    22141 tttggtattg catgggcatt tatatatttc tttgttttta cattctttat caaaaaattt
    22201 aaggtagcta ttcctggaat ggaagaagaa tcagaagttc attctgaaaa agtaataaac
    22261 aaaaatacat cattacatga agagtctctt atcattattg aagctcttgg tggaaaagaa
    22321 aatattgagt ctgttgaagc ttgtgcaact cgtttgcgtg ttgctgttac gaatggggag
    22381 attattcaaa aacaaacaat aaaagacact ggtgcaactg ctgtcttcga agtgaagggt
    22441 ggcatccaag ctgtttttgg agggaaagct gacttattaa gtcaagaaat caatcaaata
    22501 ttaggaaaag ataattagga ggtttttaat atgtttgaaa ttttcaaaaa gaaaaaacaa
    22561 gaaacttttt atgcaccttg ttccggaaaa atcatttcca tcacagaagt aaatgatcct
    22621 gtcttttcac aaaaattact aggtgatggt tttgccgtca tacctgatgg ttcaaaaatt
    22681 tatgctccat tggctggtcg agtaacaaat atatttccta ctaaacatgc tattaatata
    22741 aaaagcaact caggcattga ttatttgatt catattggaa ttgatacagt tgaattacaa
    22801 ggagagcctt tttcgatttg tgttcaagaa aatgatgagg ttactcctga aacctcgttg
    22861 gttgatgtgg attttgacaa aataataaaa tctggtaaag atacttctgt tatagttgtt
    22921 tttccggaga aaaatcagtt caatgattta ataatagata atggtcgaat tgctcatggg
    22981 aattcatgtg gaaaaatcac tttttaaaaa agtgtatata aaaagctata aaataagcta
    23041 aataggatat ttgtcaaatg aagttggtga acagaaagaa ctgttttctt tctgttcatt
    23101 tttttatttt attagtaatg attccccatg ccaaatcatg atccgtaatt taaagttata
    23161 aaagcttcga aatccattgg catttcgttg gaataccttg atatgattat tcaaatataa
    23221 aatatagaga gaagagagag agggaagata tttataaagg gcaaagttat gtttactcaa
    23281 cttaggaaat agaaatagat caatttatat tctcttgtca atcgaatatg aaccttagca
    23341 ctatatacca aatatagtaa aatgctgaaa gatgt
//
LOCUS       DAZUMO010000040        23177 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000040 DAZUMO010000000
VERSION     DAZUMO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.040"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..446
                     /locus_tag="ACG8ER_001647"
     CDS             234..446
                     /locus_tag="ACG8ER_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050226.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            453..677
                     /locus_tag="ACG8ER_001648"
     CDS             453..677
                     /locus_tag="ACG8ER_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050227.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            704..1237
                     /locus_tag="ACG8ER_001649"
     CDS             704..1237
                     /locus_tag="ACG8ER_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS5050228.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            1224..2468
                     /locus_tag="ACG8ER_001650"
     CDS             1224..2468
                     /locus_tag="ACG8ER_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFS5050229.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            2481..3980
                     /locus_tag="ACG8ER_001651"
     CDS             2481..3980
                     /locus_tag="ACG8ER_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5050230.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            3985..4911
                     /locus_tag="ACG8ER_001652"
     CDS             3985..4911
                     /locus_tag="ACG8ER_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFS5050231.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            5038..5640
                     /locus_tag="ACG8ER_001653"
     CDS             5038..5640
                     /locus_tag="ACG8ER_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFS5050232.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            5653..6576
                     /locus_tag="ACG8ER_001654"
     CDS             5653..6576
                     /locus_tag="ACG8ER_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5050233.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            6594..6857
                     /locus_tag="ACG8ER_001655"
     CDS             6594..6857
                     /locus_tag="ACG8ER_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050234.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            6869..7198
                     /locus_tag="ACG8ER_001656"
     CDS             6869..7198
                     /locus_tag="ACG8ER_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFS5050235.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            7195..7521
                     /locus_tag="ACG8ER_001657"
     CDS             7195..7521
                     /locus_tag="ACG8ER_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050236.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            7505..7843
                     /locus_tag="ACG8ER_001658"
     CDS             7505..7843
                     /locus_tag="ACG8ER_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS5050237.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            7843..8235
                     /locus_tag="ACG8ER_001659"
     CDS             7843..8235
                     /locus_tag="ACG8ER_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFS5050238.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            8246..8758
                     /locus_tag="ACG8ER_001660"
     CDS             8246..8758
                     /locus_tag="ACG8ER_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFS5050239.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            8804..9172
                     /locus_tag="ACG8ER_001661"
     CDS             8804..9172
                     /locus_tag="ACG8ER_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFS5050240.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            9226..9513
                     /locus_tag="ACG8ER_001662"
     CDS             9226..9513
                     /locus_tag="ACG8ER_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050241.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            9530..12649
                     /locus_tag="ACG8ER_001663"
     CDS             9530..12649
                     /locus_tag="ACG8ER_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5050242.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            12661..13449
                     /locus_tag="ACG8ER_001664"
     CDS             12661..13449
                     /locus_tag="ACG8ER_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFS5050243.1"
                     /translation="MDQRENKMYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGY
                     QTLTVEGREMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELL
                     NYLYRNEDVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVS
                     DGYIGNPIQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQV
                     TVNGEDCTSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            13446..16277
                     /locus_tag="ACG8ER_001665"
     CDS             13446..16277
                     /locus_tag="ACG8ER_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5050244.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            16252..18009
                     /locus_tag="ACG8ER_001666"
     CDS             16252..18009
                     /locus_tag="ACG8ER_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS5050245.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            18026..18808
                     /locus_tag="ACG8ER_001667"
     CDS             18026..18808
                     /locus_tag="ACG8ER_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050246.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            18808..20994
                     /locus_tag="ACG8ER_001668"
     CDS             18808..20994
                     /locus_tag="ACG8ER_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS5050247.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            20997..21443
                     /locus_tag="ACG8ER_001669"
     CDS             20997..21443
                     /locus_tag="ACG8ER_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050248.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            21445..21582
                     /locus_tag="ACG8ER_001670"
     CDS             21445..21582
                     /locus_tag="ACG8ER_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5050249.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            21619..21912
                     /locus_tag="ACG8ER_001671"
     CDS             21619..21912
                     /locus_tag="ACG8ER_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050250.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            21935..22132
                     /locus_tag="ACG8ER_001672"
     CDS             21935..22132
                     /locus_tag="ACG8ER_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5050251.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22143..23162
                     /locus_tag="ACG8ER_001673"
     CDS             22143..23162
                     /locus_tag="ACG8ER_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5050252.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
BASE COUNT         8131 a         3768 c         5042 g         6236 t
ORIGIN      
        1 taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagtgact ttggcaactg
       61 agggttggaa atgggcgctc aaagtacacg agcaaggcga ggtcgatagt aatcgatgga
      121 atcggtgtag gttgcttgat agaactataa ctgcatctcg tttatgaggt gtagttttta
      181 catattagat cactcatcga gtggtctttt tattttggaa ggggagtaaa aaaatgaacg
      241 aaaaccaatt aagagagttg tttaaaacga atgaagcaaa caaaacaatg gaggcgacat
      301 tctacgaaac tcaaaaaagc ttagcgttaa tcgcaaaaca agctaagtat ttctacgatc
      361 agcttattct gcaaggattt aatgacgcac aggctatgga atttatgatg cgaacctttt
      421 ctgccagtaa ccaacagaaa gagtgatacg taatgaggaa ctactggtat gtatcattat
      481 caaataaata tccgcaacca aacgcagatg atccaattag agttgtccag tcagtccaga
      541 ttaaaaagaa gtactccatc attgaaatga ccagagaagc tacgccaaaa gagattgata
      601 agtacaatct tcgttactgc ggccatggat attttagtga gcagaacata cagacaaata
      661 ttgagagata ttgttaataa aattgaaagg tggtggcttg aatgtggtaa atttgacacc
      721 aaaacaaaaa gcttttgcgg atgaatatat aaaaaatggt ggtaatgcta ctcaagccgc
      781 cattaaagca ggttatagca aacgatcagc aagagtaata ggtaaggaaa acctaactaa
      841 acctaacata atacagtata ttaatgaacg gctaaatcct atcgaaaaga agcgcaaatt
      901 aagtgccgag gacgctttaa atgaattgat agatatttgg caaggagaag tacaaataag
      961 cgtgagcaag caaatagacc gcttggagaa aaacaaggtt attaaacata tgcaatatga
     1021 atatacacca gatttagaaa gcaaattgaa agccttggat ttgtatttga agtataaatc
     1081 gctgttatca caaacgcaat tagaaaaagc tcaaacagaa ataaaattaa tgcaagcaaa
     1141 attagaacaa ttacagataa actcagagcg ttctaccgaa gaaaaacttg atgagttgtt
     1201 agaaaagatt agtggtgaat tagatggcac tagttgatat ttataaccca aagcaaatcg
     1261 acgtgttaaa taaaaccatt aagaatgatt ggtttattac tttattacat ggagcaaaac
     1321 gttctggaaa aacaaaaata aacaatgatt tgttcttgtt tgaattgcga cgtgtgcgaa
     1381 agattgccga tgaagaagac attaaggaac caatgtatat cctagcagga gtttcaagtg
     1441 caacaatcca aaagaacatc ttacaggaac tatacaacat gtacagcata gaacctaaat
     1501 tcgataaaca tggaaacttt aagctatttg gcgttaaggt cgtacaagct tatacaggaa
     1561 acattggcgg tgttggcgca attcgtggta tgacagcata tggcgcttat atcaatgagg
     1621 catcgctagc tagacaagaa gtatttgctg aaatcgtttc acgttgttca ggaacaggag
     1681 cgagaatact agctgataca aaccctgaca acccggaaca ctggctaaag aaagagtata
     1741 tcgacaattc aagcaaaaat attcaatcgt tccactttga attagatgat aatacatttt
     1801 tatctgaacg atatcgtaac aatattaaag aatcaacacc aagcggcatg ttttatgatc
     1861 gtgatatcaa aggtttatgg gtttctgcag aaggtgtcgt ttatcgtgat tttgatgcta
     1921 gcaagcacta catccagtca aaagacttac cacctttgag caacttttat tgtggtgttg
     1981 actggggata tgaacactgg ggttcaatcg tagttatagg tgaaacggat gacggaacag
     2041 cttatttaat cgaagaacat gctactcaat ttgaagagat tgattattgg gtagatgtgg
     2101 caaaaggcat tcaagagcgt tacggcttac gagtaccttt ttattgtgac tctgcgagac
     2161 cggagcatgt ggctaggttt gtaagagaaa agattaatgc taaaaatgct cataaagcac
     2221 ggttatctgg agttgaggaa gtcgccaaga gatttaaaca agataaattg tttatctgtc
     2281 aagatagagt gatgaaattt cgggatgaaa tttatcaata catttgggac aagaaaaaag
     2341 gcgagccaat aaaagaatat gatgatgtgc tagactccgt tcgatatgcg atatatactc
     2401 atgagcttct taagaaacca aaagttaatg tcaacgaaaa gattaaacgt attaaacgca
     2461 tgttttaagg agtgtgaaaa atggataagg taaacgagtt tgaatacgga gctgatatac
     2521 attattctaa tgacgtgaac acaaattatg taaagtttag cgtagactca aatcttcact
     2581 atagatttag ctcagcagaa gatttactta acgatttaga tactttagca gcaatgataa
     2641 aacatcatca tgaatatcag gtaaaaaggc taagtgtatt agatgattat tataaagcta
     2701 gaaatacaaa tatcatggat aaccgtaggc gcagagaaaa agaaaaagcg gatcaccgat
     2761 cagcacataa ctttggaaaa gttctttgta cgtttgatgt tgggtacaac acaggcaatc
     2821 ctataaaagt gcaaatcgag gacacaaatc aacaaaaaga aatcgaagag tttaatacta
     2881 ataatgacat agatgggtta aatgctgaac tctggcttga tatggataag tatgggagag
     2941 cctatgagat tatctatcga gattcagatg atacagatta tgttgatttg gctaatgtat
     3001 ttgaaacgtt tgttgtatat gatactacag taaaacgaga gcctattttg gctgtacggt
     3061 atcctaagac aagattcaac aaggatgctg ataaacagta cattcaacca atcgtataca
     3121 caaaagaaaa aagtatcact tatgatgaga cgacactaac agcaattgag ttaaagaatc
     3181 cccaggatga accgcatgaa tataaagagg tacctattac agagtattct cctaatcgtt
     3241 ttcggatggg cttgtatgaa gatgtactat ctttgattga tctatacgat gcagggcagt
     3301 ctgataccgc caattatatg actgatctaa acgatgctct tctagttatt agtggtgata
     3361 ttgaagcagc aggactatcc acagaggacg ccatcaagca gaaagaagcg aatatgcttt
     3421 tgcttgagtc tggaactgat gtgaacggta ataaaacaag tgtgactgca ggatatattt
     3481 acaaacaata tgatgtgaac ggtgtagaag catacaaata cagagtacgc aaggatattc
     3541 acgaaatatc catggttcct gatcttactg atgacaattt ttccggagtg caatcgggag
     3601 aagcaatgaa atataaatta tttggatttg aacaaatgac ggcaacaaag caaaggctat
     3661 tcaaaaaagg ccttatgcgg cgttatcgtc ttttatttag cctaaaatca agtatttctg
     3721 aaatggataa ctccgatttg aaaggcttac gtgtaatatt tacgcctaat ctacctaaag
     3781 ccattctgga agagttgaaa tctttggttg atgctggagc tgaactcagt caagagacga
     3841 tcttaggact cgcttctttt gttccagatg tacaggcaga gttgaaacga gtaaataaag
     3901 aaacgcaaaa gcagattggc atttttgatt cagatggtga agaagtaatt aacaacaaaa
     3961 aagatgaaac aggggggtga ttaaatgaac tcccaagaat attggatcaa acgggaaaag
     4021 gaatggcaaa agcaacaaat taaagatgat aaaaagcgca tggcagaaat taaaagtcgc
     4081 atgcaatacg cacaagatgc gatacaaaaa gaaatagacg cgcagtggga cagtttctcc
     4141 aatggccaga aaatcactcg tagcgaagcg atgaagcgtg ctagtgaaat ggatgtcaaa
     4201 gcattcgctc gcaaagcaaa gaagtatgtc aaagagaaag atttttctcc tacagcaaac
     4261 caagaattaa agctatacaa tcttacgatg cgtgtaaata gattagagct cttaaaagct
     4321 aatatcgggc ttgaattgat ttcactgttt aatgaattgg ataagtactt ttcgaatgaa
     4381 ttaacaaaag ctggtttagc tgaattgaag agacaagccg gtattttaga aatgactatt
     4441 gcttcaagtg gatatgcaaa gctgatagaa ctagtaataa acagctcctt tttgagtgat
     4501 gacgtgtctt ttagtgatcg cttatggatg tatcaatctg aattgaaatc agaattagat
     4561 aggttgttag tcagaagtat aacgatgggg aaaaatccca agcaacttgc atctaaattg
     4621 gcagaatatt taacagctga aggacgagaa aacactaagt tcaacactca acgtttgatg
     4681 gtgactgaaa cgactagagt tcaggtaggg attcaagaac gaagttatag agatgcaggc
     4741 attacccagt acatctatat agcagaacca acggcgtgca aactatgtat accgttaaat
     4801 aatcaagttt ttgatgttgc cgatatgcag ccaggaagta acgctcctaa tatgcatcca
     4861 ttttgtcgat gcagtacagc accttatata gaacgaatat caagccgtta acacaaatta
     4921 acggcttttt attgtgcctt cttacagctt acaggcgtta aagagaaagc tattttcggc
     4981 tgaccggcgt aactggtcaa atttatcggg tagcggcgta accgtggagg attaatcatg
     5041 aaaaaacgtt tatttatgcc aatgaactta caattttttt ctgaaccagg agatggtgga
     5101 tctggtgatg agggacaaca aggaaaccta ccagctggct cacaagagac accgcccgaa
     5161 gcaaaagaag aaaacaatac tggcaaaaca ttttctcgtg atgaagtagc gaaaatgatc
     5221 gctgctgaga cgaataaagc aaaagcagcg tgggaaaaag aactagcagc aaaaaaagaa
     5281 gaagctaaaa agctggcaaa aatgaatgcg gaagaaaaac tacagcatga gttggagcaa
     5341 aaagaagctg aaatcgctga attaaagcgt ggacaggcac tatctgaaat gacgaaagaa
     5401 gcttctaaaa tgctgacaga tgcaaattta ccacacgatg atgatttact tggtctgatt
     5461 gtttctgatg atgcagatgc cacaaaacaa gctgtagcag tcatcactaa ctttgcttct
     5521 ttgattaaga gagaaaacgc aagacaaaca ccaccaaatg aaggtggaca atttacagca
     5581 tcgaaaaata ctaaagaaac agtggctaaa ctagctgcta aaaatcgaat tatcaaatag
     5641 gaggaaaact taatgaaaaa gaaacaactt ttaccaatga acttgcaaat gtttgctcaa
     5701 acatgggatc cagataatgt cttggtatat gaaacgaaag agggaaaaat tcctgataaa
     5761 tataatacgc tcattttgag tgaagttatg gaaaattcta agatcatgca gttagcaaaa
     5821 tacgaagaaa tgactgacaa agaaaagaaa tttgaatact ttgcagaagg accaggcgca
     5881 tactgggtgg gtgaaggtga aaaaattaaa acgtctaaac ctaaatggat gcaagctacg
     5941 atgactgcaa aaaaactcgg tgtcattctt ccggtttctc gtgaatattt aaattataaa
     6001 ttatcagatt tctttgagga gatgcagcca aaaattgctg aagctttcta taaaaaattt
     6061 gatgcagctg ccttattaaa taaagaaaac ccatttcctc agtcactaga cggatcagtt
     6121 attagtgcgg ggaatgtggt tgaaggcgga ttgacttatg ataatatcct agccttagaa
     6181 gacaagttag cagaaaatga attcgaacct aatgcgttta tttcaaaccg aaaaaatcgt
     6241 acagaattac gttctgcagc tcaaacagtc gggtcaaatg ttgagtttat ttatgatcgc
     6301 tctactaata caattgacgg attaccagta gtagacctta agtctttaga taaaggaaca
     6361 ctttacgctg gagactttaa ttacatgttt tatgggatcc catataatat ttcatttaag
     6421 atttctgaag aagcccaatt gtctacttta actaatgaag atggaacccc agttaacttg
     6481 tttgagcaag aactgattgc tttgcgtgca acaatggatg ttggatttat gattgtaaaa
     6541 gatgaagcat ttgggaagat ttccccaaaa gcgtaacgcc tgctaccggt attgtgccaa
     6601 atcaaaagac atggaccggt aaagtaggcg atactaaaac atttactatt tcagctgtgc
     6661 ctgcagatgc tagcgatgca gctgctgttg ttgcagctac tacagcaact tcaagtgatg
     6721 gagctatcgc aacagtgacc aaaaatgaaa atggtggttt tgatggaacg attgcagcag
     6781 aagggtcagc aacattcaca tttacttctg gagaattcac tacttcaatc aatgtgacag
     6841 gtcaacctgc tagttaggaa gtaaaaatat gatgattgca gatgatatta aaaaacttct
     6901 taaaggaaca atagatgaaa agcttgaagt tattgagcga agaacgaatg agcgtatgaa
     6961 aaccttgtta aatacgcaag aagttcctaa agaatttgaa acagttgtat atgaagtatc
     7021 gttgaaaaga ttcaatagaa ttggtcaaga aggtatgcag tcatattctc aagaaggttt
     7081 atctatggct tttcctgact cggatttttc agagtatcaa aatgagattg atgaatttaa
     7141 gcgtaaagat caggaagagt tgtacaagcc aaagcgaggg aggtttaaat ttgtatgaga
     7201 tttacagatg aaattatatt tgttaaacgt tcatctgact ctaaatatga tccagatctc
     7261 ggtgagtggg ttgaaggcaa accagaaaga acaagaacag aggcaaacgt gacagatatt
     7321 ggcactgata gaagtgtgac tatttttggt agtgtggaag aaggggcgaa ggtcattagg
     7381 acgcagcctc ttttttctat ccctacattt gactatatcg agattgaagg aaagacttgg
     7441 caacaaaaaa cagctagaaa tccagcatat agaaatagtt taattgtgca agaggtggtt
     7501 cttgatgaag gcacaacttg aatataaagg aatcgatcag ctgatgcgac atctgaaaaa
     7561 agcagcaacg cttaatgacg ttcaaaaagt cgtgaaaagt aatactgctg aaatgactga
     7621 acgaatgcaa aaaggtgcgc cagtggatac aggttactta cgaagatcaa taaacatgaa
     7681 tcttttagaa gctggtttaa ctggtattgt aggaccgaca gcagattatg caccttatgt
     7741 agaatatggc actcgattta tgtcggcaca gccttatgtt agaccagcgt ttaattacca
     7801 aaaagtcaaa tttatggctg aaatgaaagc cttggtgaaa tgatgattaa gacaagagat
     7861 caatcaattt ttgatgaact ttttaaaata tcccaaaaca aacttggata taaaacatac
     7921 gattacaaaa ctttagagga tgttggttat ccttttgtgg aatttgagaa cactcagaca
     7981 atccatgaag taaataaaac tgacattaaa gggtctgtga ttgtggtttt atccgtttgg
     8041 ggattacaga agaaacgaaa gcagttgtca gatatggcat ctgctctttt taatgaagct
     8101 agattgatag aagccacaga aggctattat tgggctttaa attatcaagc aagtggaatt
     8161 caagtgatgg acgacacaac aacgaataca ccgctaaaac gggcggttgt cacacttgaa
     8221 tttagaatta gataggagga agaacatgga agcattaaaa ggtattgatg tcattttgct
     8281 ttatcgctta ttgaaaaaag aaactcagga agccgcttgg aaaatggcat ttcaaacaga
     8341 acacgaaaat ggtttatcaa gagattcaga ctctacagtg acaaaagatg gaaacgttca
     8401 aagtttaagc ccggttgaat atgatttttc ggctacttca atagttgcta aaggagattc
     8461 tcatgtagat gaaatgaaac aagctttatt aaatggggat atcattgaaa tttgggaaat
     8521 taataaagca gaacagggaa cagatgataa tgcaaataag tacaaagcta cttattacca
     8581 agcatatgtg tctgaattta ctccatcggc tgctgcagag gataacgttg aattaagttt
     8641 atcatttgca gtaaatggtg ttggtcaaga tggttatgca accttgacag aagatcaagc
     8701 cgatgttgtt caatatgcat tcaaagatac cgtgaaagca acttcgacag gagcataaga
     8761 gggcttagat gctctctttt ttattttagg aggatgaaaa acattgaaat taaaaattaa
     8821 aggtaaagaa tattcgttta aatttggcac taaatttgta cgtgaattag ataaagtgat
     8881 gcctttcgtc gatggaaata tggaatttgg aatgggactc tccgcaaaag tcttaccaga
     8941 gctacgttct tataacgtta acacgttatc acgagttctg gaaatagcaa atagaactga
     9001 agatgaatct attacattag atgaattgga tgattacatc gatgaagtta aagacattga
     9061 aaaattgttt gatgaagtcc taaaagaatt ggcggagtcg aacgcgggaa agttagcggt
     9121 ccgaaacctg aatcagaaat tgaaagaagc ggaaaaacaa caagtggaat agattctgca
     9181 ctggcatacg aacaaattct tatcaattct tttcgatatt tgggaatgac caatatctca
     9241 gatatcgaaa gaatgacgtt atatgaatac aacattcgta tgactgcagc ccagttatct
     9301 tggcttgaca aagaaaagtt gattcacgaa ttagcgtggg caaatcagca agtccaagcg
     9361 gagaaaaaag taggcaaaaa gacagttcct gtatatcgat cctttgaaga attcttcaat
     9421 tatcaaaaaa tcgaagattc aatcatggga gtttccgaac tttcaaaaca agataaaaaa
     9481 ttccaaagct tactaactaa agctaactct tgaggaaagg aggagaatca tggaacaatt
     9541 ttctgttgaa gccttattaa aagccacaga tagtggattt gtaaagactt ttaaagatgc
     9601 acaagatgct gttaaaactt ttgaaaagaa ttcaaatagt atgacaaccg ctgttggtaa
     9661 agtgatgcaa ggtactggtg ccgcaatgac aaagtatatt accactcctc ttataggagt
     9721 aggcgtagca gctgctaaag ttggtggcga cttcgaagca caaatgagtc gtgtaaaagc
     9781 tatatcagga gcaaccggcg acacattcga acagatgaaa cagcaagcga ttgatctagg
     9841 agcaaaaact gcttttagcg caaaagaatc agctgctgga atggaaaact tagcttctgc
     9901 tggatttagc gcacaagaaa tcatgaaagc aatgccgggt cttttagact tagcagctgt
     9961 atctggaggg gatgtggctc tagcttctga aaatactgct actgctttga gaggatttgg
    10021 tttagaagca agtgaagcag gacatgtcgc tgatgtattt gctcgtgctg ctgcggatac
    10081 caatgctgaa gttggagaca tgggagaagc attgaagtat gttgctcctg tagctaattc
    10141 aatggggatt tctttggaag aaactgcagc agctattggt attatgagtg acgcaggcat
    10201 taagggttct caagcaggta caacgttgcg aggagcattg tctaggttag caaggccaac
    10261 aaaggctatg caagatacaa tggataattt aggtgtttcg ttttatgatg ctgacggtaa
    10321 aatgaaacct ttaaaaactc aagtagaatt acttaaaaaa gcttttgaag gcctgacgcc
    10381 tgaacaacaa caaaatgctt tagtaacact atatgggcaa gaatcattat cagggatgat
    10441 ggctttgatt gataaaggac ctgattcatt gggcaaatta acaaaatctc tgaaagattc
    10501 tgatggtgca gctgacgata tggctcggac catgcaagat aatatgaatt cttccatcga
    10561 gcaaatgttt ggagcttttg agtcagcagc tattgtaatt caaaagattc tagcaccatc
    10621 catcaaaaaa gtagcagatg ccatatccgg cttagtagaa aaatttgtaa gtgctccaga
    10681 atcaactcaa aaattagtag tggccatagg agcaattgtc gctgctatag gaccgttaat
    10741 ttttatgatt ggttcagtaa ttatatggat caatagggtg aaagtagctt ttaaagcttt
    10801 aagtgaaagt tcaaaattgt ttagtggatt aagtaaagca atgggtcttc ttacaaatcc
    10861 ggtttttctg gttatagctg cggtagcact actcgttgta ggtttcatct atctttggaa
    10921 tacgagtgaa gattttagaa acttttggat tggcttatgg gagggaatca agtctgctgt
    10981 aagctcggca gtagaatgga ttcagaatgc atggaaatct acaggagaat ggtttaacaa
    11041 tttatggaag tccattaaag aaggcgcaga caatgtttgg actacagttc aagaagcccc
    11101 tgggaaagcg gcagattgga ttaaaaacaa atggactgga acaaaagagt tcttttcaaa
    11161 tttatggtca agtattgcaa actctgcttc agagatttgg aatagtttaa aagaaggtgt
    11221 catatcagtt attgatgatt tagtttcaag tgctggtgaa aaatgggaag ggtttaaaaa
    11281 tactatatct actgcatgga aaacaattac aagtaaaatc aaatctggtt ttgattttat
    11341 actaaaatat attggtccat ttgtaagtag cttttcagat gtgttctcta atatagtgaa
    11401 agcaataaca agtatatttg ctgaggttaa aaacataata gtaaatgctt gggaaatcat
    11461 taagtcttta atagctgcgc cactactgtt tattatagat ttaattactg gtgacttcga
    11521 acaaatgaaa gaggatttag atctaatctg gaacacactt gtccaatcag tggtaaatat
    11581 ttggacatct gtaaaaaata tatttacgga atatatcggt gcaatagtaa atagtgccgt
    11641 tagtttatgg actggattca tacaaagtat ttctaatatt tggaatgaag tagtttatca
    11701 agcgactatg atttggattg atttgaaact attttttact aatttatgga ttgatattaa
    11761 atacagtgca attcaaatgt ggataaatct aaaattctcc ataattcaaa cttggattga
    11821 tacaaaatat ggtgcaattg aactttggaa taatctaaaa caatggtttt tccaaacggt
    11881 taataatatc gtgcaaactc ttataaaaag ttggaacagc ttaaagcaag gaacgataga
    11941 tttattcaac aatacagttc aaggtgctaa agacatttgg acttcattca aatcttggat
    12001 tggtgattta attactggaa ccaaagataa catcattcag ggatgggaaa acctaaaaca
    12061 aggtactata gatactttca acaatttagt aaatggtgct caagaggcat gggataattt
    12121 agtaaatgct gttagtgaca cggttgatag agtaactggc tggtttgata acttgaaaaa
    12181 tatcgattta ctagcagccg gaaaagctat catggatagt tttctagaag ggttacaaaa
    12241 tgcatggaaa tctgtgcaag attttgttgg aggtattggt gattggattc gtgaacacaa
    12301 aggacctatc caatacgata gaaagctatt gattccagct ggtcaggcta ttatgaacgg
    12361 tctgaataaa ggtctgacag gaggattcaa tgacgtacag aatactgttg gaagtatggc
    12421 agactttatc gcggaacttt tcaatgcaaa ttctgatgta gatatagctg caaatttgaa
    12481 aaatgcaaat aaaaacattg gtgcacaagt tgaacataaa ataaatatgg gtggctctac
    12541 taaaccagct gtatttaaaa tcaatcttgg aagacaatcg tttagattgt ttatggacga
    12601 tatttcacaa gctatgggcg aaggtgcaga tattaatctg gaattttagg agggaatatt
    12661 ttggatcaac gagaaaataa aatgtactca ttcaaagata caaccattaa tctcactagt
    12721 tctaaacgat tccttccgac gtctgccatg atgtacgatg gaatgtattt agaagatttg
    12781 attgatggtt atcaaacact tacggtggaa ggtagagaaa tgctttctgt agaagttgaa
    12841 cagcaagaga tacaaattgg ttcaatcatt acaaatcaga aaataccttc aagaacacta
    12901 aaaataacat ataagctgga agatagagat ccagaaaaac tacagtttaa attcaaagaa
    12961 ctgttgaatt atttataccg gaatgaagac gtggaaatta ggtttcatga tgaattagat
    13021 tattattact acggtcgcta tacatcaact gatactgttc caggagactc caactcgatt
    13081 atttcgagtt ttaatgtatt ctgtgcggat ccactaaagt atacgaaaga gtgtgttagt
    13141 gatggctata ttggaaatcc gatacagttt cctataacac caagaaaaat tgaagttact
    13201 ttatccatga ataattcaat caaaattaca aacggagaac aaaatatcac gataactgac
    13261 gcggcaataa aaacaggaga cgtgttggtt tttgattttt ccgatgagca ggtaactgta
    13321 aacggagaag attgtacttc tatgattgat ttagaaagtg attttgagaa cttttatctt
    13381 aagcaaggtc agaagataac tagcaataat gggaagctta aaatattcta taggggggcg
    13441 acaatttgag tgagacagtt tatttctttg atcacttgca aaaacttatt aaaagaaaaa
    13501 atacaagaag tttgattgaa gtctcccaag aaaaagaaat tagttctgat aagagtgatc
    13561 taatgaaaga tactctttac gttacgacaa aatatgataa agaaatagag gatgcaagat
    13621 atatggcgat tcgtgaaaac gagtcgtctt tttcgttgta tcgaattact aaagttagcg
    13681 acccatttga aacattagag tttacagggt taggatttgc gacaaatgaa ttagatgctt
    13741 acatcatcaa agatattagg ccgagtgggc agcccttaaa aaatgttctt gatcgattga
    13801 ttgaatttac tgaaggaaat tggcgcgttg gtcacgtaga agcaatgtta ccaacagtaa
    13861 ctgcaacttt ttactatgtc tctgtaaaag aagcgttgaa agaattgcaa accttaggta
    13921 tggaatttgt ctttaggtgt tctttgaatt ctgatggaat aaaggataaa tggatcgaag
    13981 tatatgaaca aattggcgaa gaatcgaata cacgtttcgt atatggtagt aaagcattaa
    14041 cagttgtaag agagatagat agaagctcaa tctcaacttc aatgataggt cgtgggcgag
    14101 gcgaagaggt tggtgacggt tacggtagaa gaattgaatt cactgatgtt gaatggaaaa
    14161 agtcgaatgg tgatccttta gataagccta aaggccaaaa ttggcttgaa gatccggaat
    14221 caactcaaaa gtatgggata ccacaaaaag atggatcaat gagaaaacga gaaaccgtag
    14281 tagtgtttga tgatatagat gatccaacag atttacttaa aaatacttat tcaaccttaa
    14341 tcgattctgc tagaccgtta gtacaattca aagctgaagt cactggagga gatgtgatag
    14401 gaaatacagt gactattcac agatacgata aaggttatca ctataaaact cgtatttata
    14461 aaactacatt caatcggctt accggtcaaa cgaatatcga actaggggat aatttaacac
    14521 aagatgttag aaaacaaacg gcttctattg tcaataatat taatagttta gaatctagca
    14581 aaatgacatt ttacgaatcg acagagattg gaaaatatca agatgacatt atgcgaggtg
    14641 caggagataa tggcggttct atttattggg taaatggaat tgaagctggt gttagtgata
    14701 gtagagaaat ctatgaaact gtttatatgg atggacctaa cattcctaga tcacgctttt
    14761 ttatggtcca aaataactca gggatatctt tcaaacaatg taaaaaaggt gagtggcaaa
    14821 caattcaaga tgtacacaat ggtgatagca cgaccgcttg gacgttggat ggaactttca
    14881 atgctaattt tattcgcgca ggcattctgt ctggtatcct cgtgcaaggg gtagctttaa
    14941 aaacatttga cgataaagat ttccaattag tggcagaggg gggagaactt tcttttgaaa
    15001 aacaagtcaa gtctactgga ctagatgatg tacatggaga acgcttgggc tcaattgtat
    15061 ctacttatgg ttctaaaggg attaacggct ttgctgtctg gaaagagcca aactatattt
    15121 tttccattaa tgctggggac ggtggcgatc gagggaatcc tatttttcaa attccagcag
    15181 atgttactgc tgataagcgc aaatataatc tttatggtga cggcaatttt tcagaaggaa
    15241 atataaccat agatggacgg ctagatgtaa aagaactcta tgttaacggt acaaaaattg
    15301 acactaatgg aggaggcaat tccggaggga acgataacgg ttggaacgga caatacccac
    15361 cagaagtaac tactgatcgg gataaacgat attggcaaat ttgggcaacg gcaataggtg
    15421 ccggttttac acaacaagct gctgcagcct tacttggaaa tgcacaagga gaatcagatg
    15481 ctaatccaac cgctgatgag ggcaatggcg caccagggtt cggttatggt gtatggcaat
    15541 ggaccgattc ttctggcgca actagcggac gtgtctacat gatcaattta atgacaaagg
    15601 ctggcatcag tgatgatcca gacacaatca cggcacagtt taaattgttg atgtggcatg
    15661 caccaaatgg tcaatggatc gcaactagcg cttatcctta cacatggaca caattcatga
    15721 atctgaccga tatcaacaca gcagcacaag cattcgtggc taactttgaa cgtccacgtg
    15781 atccacatcc agaacggacg gcatggtcac aagaatggta tgacaagttc aaagatttgg
    15841 aaattcctgt atcaaaagga tatatgaagc cgattgcgga tccaatcaca gtgacgagcg
    15901 aatttggctg gcgcacttct ccaatcacag gagcacaaga atttcataac ggtattgacc
    15961 ttgtaaatgg aaatcctaat acacctattt ttgcttcagc ggatggcgaa gtgattgttg
    16021 caggtgatgc aaattacttt gactggtatg gaaattggac agtgattaaa cacgctgatg
    16081 gaatgtatac aggatacgcg catcaaagcc gagtagacgt ctcaaaaggt cagaaggtaa
    16141 ctgctggtca gcaaattgga ctgatgggga caacaggacc atccactgga gaacatcttc
    16201 attttcaatt tatggatgag ttttatccat cttcttcagg tcatttccac aatgcaagag
    16261 actacatcga tttctaaagg agggatagtc atggcagaaa cgcagcataa aatggtccta
    16321 tccaccaccg aaccaaacaa cggaataaat ttggttcgaa ttcggcaagg ggatgtttta
    16381 acgcaaaagt tcgttgttga agtggtggaa catggcaaac taaaaacatt cgagggccta
    16441 gtgccgtttt ttattaatac aacaaaattt ggcgaaaacc aacctgttga acaaaaagta
    16501 caagaataca gtccagcgca agcaaggctt gtttacacct taagtgagcc tgactggcaa
    16561 tggggtggtg aaaacaccgc acatttcagt ttccgatcac ttaatggtga tggaacttgg
    16621 agtgaacaat ttagcacaca ggattttacc tatcgagtca tttctggaat atctagaagc
    16681 cagttacgtg actctggcta tgtgtggaca tttgaggatt tgctaagaaa attcaaagat
    16741 tacatggatc agggcaaaaa tgactgggag cagtggttag aagataatcg tgaaatactg
    16801 gaaaatatcg atccaggtgg tatgatcatt aacattctga atgaagctaa aggcgactac
    16861 gaatcattag ctgatcgttt aaaccaaaaa taccaagtgc cagtcggcag ctcacaaatt
    16921 agagaaacaa cacgcttttt tgattacgac acgatgaaat acgttgacct agtgccgcgc
    16981 aatttgaata cggttgtcaa cagtgttaat aacagcaaat ttaacttttc tttcattaca
    17041 gacattcacg cagataatca caacttgcgt atagatggcg tcggttacaa agatgcttct
    17101 tatttaagac attggcgcgc aatccctcaa tttcaaaaat tagggaacaa aacagatgtg
    17161 atgatttacg gcggagacaa tatcgacggc ggacttggtt cactcggcag cgatataggc
    17221 attattgatg aatggagcgc gcgacattcc atgttaggca cgctcaaacg cttcactaac
    17281 gcagcggtag caggacaaga aaaaccggtt attatctgca aagggaatca cgacgcttgt
    17341 ttcgaccccg cgtggcgcaa gcgaaaggga atgttatgca acgctgactt cgaagagtat
    17401 tggaacggtt tgtacggtgg cgtgttgttc ccagataaaa acgcagcaat ttaccgtttc
    17461 gatacttgtg atttttatga aggcggcaca ggcgacaagt acgtggacgg ttacagcgat
    17521 acagcgccgg gagctttcag tgccaaacaa attaacgctt tcggagaatg gttagtgaac
    17581 gttccgagaa actatcatgt ggcgttagta ggacatacgc ctttaggtct cagcaagttt
    17641 cccgttcgca atgaaaacat gattagcacg ttgatagaag ggtttaaatc tggtagtccg
    17701 gtaactatcg attggtctaa attaggacaa ccgaatgacg gttcgttcgg cggattaaag
    17761 accttcgcaa tgaatacaaa aggcgccggc gttgtagtag gttatttctg cgggcattgg
    17821 cacgaacaag tcgaaggggc gtttggaaca gtaaaaatga ttctttgcga cgtcggcttc
    17881 tctcatacag ctagccaagt tgatacaccg gatgaattag cgttttataa aatagaagtt
    17941 gacacagcaa caagaaaagt gacaagcaaa ggcgtagggc gcgcgagaga cttcacttat
    18001 aactattaag gcggtgaaca attaaatgtt agattttcaa agcaaaccaa atatttttga
    18061 agaaatgagt tatgaagagg ctgtgaaatg gttattgcgt caagcggcta tccattacga
    18121 cggctcggat cacgacgcgc acgtcctagc tactgaaagc aacgcgggct ttgctacacc
    18181 ggaaacagtt atgcaagcgc gtgggcgttg gttacgcgat tataagctac cacaaaaata
    18241 tccgaacatt ttagacattc cgcccggcaa atacgcaacc aaagccggat ggggcgcaga
    18301 caaccctgac gggattgaag atgacagttt tgttgaaatg atggtattcg cggatcacga
    18361 ttcgagaaaa ttaatcgtcg cttttgctcg ttatagcggc gaaatttaca tcaaaatgac
    18421 acataacagc gaaccggtag agggttataa ctcgttaggc tggcggcgcg tttacactac
    18481 ttctgttctt tttgaaggcg aattaagaaa aggacaatca gtcaatctgc cagatgatac
    18541 tttccgttac caaacgcttc gtatccacta cacagacggt gacggagact ttgtagaaga
    18601 agtgaaacgt caaagatacg cgcgaattac aaaagcgaat ttatggaatg gagacgcggg
    18661 agtgacgttc atcgaatttg agctgacaat cgaagcgcga aagataacga tgtcaaacgg
    18721 cagagcttta gatatttcat ccggcaatgt ttccaatccc gcaatgagca acaacgtgaa
    18781 aataactaag attgaaggtg tgaaataatg gcgcatgtca tcaaaaaagg ctctatcaaa
    18841 gtacctacac agccgaaaga ctacgatttg caagcaacgg ggcttgtatt taaatcatac
    18901 gataatcaaa tagcgttaga gttcaacgtc gaacaacaga acggcacacc ggcggacttg
    18961 ctaggagcta acttgcgctt gttgatgttt atctatgatg aagtagatgg aatgatcacg
    19021 aaagagccaa tcccttttat cacgaaaaac ctcatcactg aaagcttctt gaatggacag
    19081 gtcgtatata tcttgccaga agcgatgaaa gcttataacg gtatggtgga agcttatgtt
    19141 tacatcgagt atccagacgg atcaacaagt gataacttag gcttcacctt ccgtatgaag
    19201 cgttcagcaa tcgacggact agcgcaagat aaagcagact actttattgc agactttcaa
    19261 caattacttg atggagtcaa acaagaagca acagatgctg ttaacgcagc attaacaaat
    19321 gtggattcta cgattgaaaa ggcaaaccaa caaataaatg aatttgtaga gggagccaca
    19381 caagcaattg atcaaactgt tgacgaggta acagagcaac tacaagctac tcaaactaag
    19441 attgataccg tttctcaaaa cgttacatcg gcacaaaaca atcttaaagc agttgaagac
    19501 aagatgaatc aaaccaatca gcaaatcggc gacctcggca agctgaaaaa gatgtacagt
    19561 aacagcatcg acttcgggaa ttatgattat agtgggaatc cgaatttaat gtccaaacta
    19621 aaatcgagcg attttaacgt tggttaccac gggtcactaa cttcggataa cgaaaagcta
    19681 cattttactt ctgatggtac aggaagcatt attatgttta cgcgtattaa tacacctcag
    19741 cttgctagtg ggaaaaccta tactctgagt gcgaaagttc gatttgatga aggaactaca
    19801 ggagctattg ataaattacg tttggtgtat cgtacatcac caggagaaaa gatattattg
    19861 gaagcaaata gtacaaatat tacaacagat gatgtaggga aagaaataac aatcaaaggt
    19921 acagctaacg ttaattatca aatcacaaat ttagatcgat tttatatgag tattagcttt
    19981 gttgacaggg ataaaataaa tggcggattt aagttgtacg acatcaaaat cgaagaaggc
    20041 tcaacagcca ccccatatca gccaaactta cttgatgacc cttactggct aggtaaagcg
    20101 cctttgggtg agaatattgc taataagtct gttacgtttc caatcaaatc tagcgcctac
    20161 agcctatacc aagctaacat ggaagaagaa tttgttttag gtcaaaccta tacaattacc
    20221 atgaaggcaa ccaaaccacc tatccaaacg ttcattgttt acaacgagga tagtggagat
    20281 tatagatatg gtaacttaga gccagtagaa gggttagttg atacatgggg tctgactttt
    20341 acaccgcaga aggttggagt taactacccc aaacggttaa ctattatcca gtatccccaa
    20401 tcaacaacag gtgcatgtca aattgactgg ctcaagatcg aaaaaggcga cacacgaacc
    20461 ccgaatatta gtcagtttaa atactttggt gaaggcttga aagacagtaa caatcccaat
    20521 gattacagtt gggatgtcac acctgaatat actgaaaaag gcttgaatga tgctgttaat
    20581 gtgtatgatc ctcagagagt tgaaggtttg aaaaactttg ccgatggtat tcaaatagca
    20641 ggagataaag tgatcagtga aaatgattgc actgtttata cattaactaa agacaacagt
    20701 caatcgttta tcgatgggta tgcaacattt attaaacacg gaaaagaagt tattgtaaat
    20761 ggtacagtaa agttcaaaaa agcttatgct tttggtgtac cacttgatga tgaagtccca
    20821 gatgaatttg tcgcaaaaat tgttcatggc atgcttacag gtccgtcagg aacaagtagt
    20881 gtatcaaaag cgatgtacgt gcgaaaagac ttaggaacaa ttattacgaa tagcgaattt
    20941 gctgcaaatg aatggtttac attccatggt aattattggg taggggagga ataaaaatga
    21001 aaaatatttg gaaatacgga cgaacaggtg gagagtatgc tggacaagtg cttgatgata
    21061 tggttatgac tgttccattt accgatgttc caccactcga aggaattcgt gctgatgggg
    21121 aaccactaac gattgctgat cagatgtttg atcctaaact gaaccaatgg attgttttag
    21181 tgaatgtact agatcacaac gatttaaaca atctcaaagc gatgtatgag tcgttagaaa
    21241 atgagaacgg cgatttaaaa cagctcaacg ccaaactcat gctaagcgat gtagcgatta
    21301 aacaggaaaa tactgcattg aaagaaaaag cggatggttt agcacaaatc aattcaaaaa
    21361 cgatgcttgc ttcgttccaa aatagcaaag acattgcaga aattaaagag caactaaatc
    21421 cagcttcaaa gggaggtgag tagtatgttt agttttagcg atgtgaaaat gatgtatgat
    21481 tggggctgtt ttactgacga tcaagttcga ctattcgttc cactatgcat tacagacgaa
    21541 gaagcagaaa aaatcattaa taaagataag agcgcatctt aagtgatgcg tttttattta
    21601 aggtaaagga gttgtcacat gattaattta ggagaatggg gaacaatcgc aggatcaatc
    21661 actgcgattg tttctttgat tttattagta ataagaccag tcactgcatc tttctcgaag
    21721 attactgaaa ctctttcaaa agtaagtcac aatttagatt tgctgactaa agatttagaa
    21781 tcgagcaaat cagatcgatt gatgattcat gaagaactaa agaaacacga tgaaagatta
    21841 gatacacata cagaaaaatt ggtagaacac acacaacaaa tcaaaacttt atttagagaa
    21901 agatctcggt aaaaaagaaa ggagttaaga agaaatgatt ttacccgata agtattatca
    21961 agtcattaaa tggacagttt taacagtatt gccagctgca tctgtattag ttgcaacact
    22021 aggcaaagcg tatggatgga atggaacaga tatgacagta ctcactatca atgcagtagc
    22081 aacattttta ggcgttatca ctggcgtgtc ggcttataat ttgaaaaaat aggaggaaac
    22141 aaatgaaaaa gaaaattact attactgcga tgagcctatt aacggctctt tttttattgc
    22201 caattaatgg gtttgcctat acgattaaca atgaatttaa tttaggccca aacgaaggta
    22261 gctcacaagt agcaaataat cagtacattt tactgcatga aacggctaat gaaacagcaa
    22321 caggacgcaa tgaagcgcag tatatgcaac gttcatggac tagcgcttat actgcttata
    22381 ttgtgggaga cggcggaatt gtttatcaag tcggtcaacc tggttatgta cagtacggtg
    22441 ctggttcgta tgctaatgca aatagtcctg tgcagattga gttacaacac acacatgata
    22501 aagcaacgtt tgagaaaaac tacaaggcat acgttgaatt agctagagat tcagcaatga
    22561 aatatggtat tccacttaca ctagacacac cgtataatca accagggatc aaatcgcatt
    22621 tatgggtaac acaaaatatt tggggcgatc atacagatcc ttacggttat ctttctgaaa
    22681 tgggcgtaag taaagaaaaa ttagcctatg atttggctca tggatttacc gatgaaaatc
    22741 cgacaacttc agatgataaa ccagtcattg atccaactcg agcaggtgct gcaaatccta
    22801 ccctgacaga tggaacaaat tacgcccaca ttgatcagtt cggagaaatc gaaaacgcaa
    22861 acttacatgt ggctggttgg cacatcgcta actataaata cgagtatatc ttcattatgg
    22921 attacaatac tgggaaagaa ttagctcgag taaaagctga tggaatatat agaccagatg
    22981 taaatcaagc ttataatact ttaggaaatg ttggctatca tgtatctttt aacatgcgca
    23041 acttccctaa taagaaagtc tatgtaatga tgcgggcaac gaatgatcca gaagggaaca
    23101 ctaaaggcgg agcacaagat tttcatgata aacgctggta tttaaatatt ccgcaacgat
    23161 aaaaatagcc cctcgtt
//
LOCUS       DAZUMO010000041        22429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000041 DAZUMO010000000
VERSION     DAZUMO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.041"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACG8ER_001674"
     CDS             144..542
                     /locus_tag="ACG8ER_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5050253.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="ACG8ER_001675"
     CDS             526..1221
                     /locus_tag="ACG8ER_001675"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS5050254.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1815..2706)
                     /locus_tag="ACG8ER_001676"
                     /pseudo
     CDS             complement(1815..2706)
                     /locus_tag="ACG8ER_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
     gene            2891..3730
                     /locus_tag="ACG8ER_001677"
     CDS             2891..3730
                     /locus_tag="ACG8ER_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5050255.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3926..4819
                     /gene="sgrA"
                     /locus_tag="ACG8ER_001678"
     CDS             3926..4819
                     /gene="sgrA"
                     /locus_tag="ACG8ER_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS5050256.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTGKVNNELDLVPII
                     PTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWM
                     GVLLILIVGASYFRSLFHRVK"
     gene            5117..5473
                     /locus_tag="ACG8ER_001679"
     CDS             5117..5473
                     /locus_tag="ACG8ER_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050257.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5518..6633
                     /locus_tag="ACG8ER_001680"
     CDS             5518..6633
                     /locus_tag="ACG8ER_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050258.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6659..7099)
                     /locus_tag="ACG8ER_001681"
     CDS             complement(6659..7099)
                     /locus_tag="ACG8ER_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5050259.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7259..7765
                     /locus_tag="ACG8ER_001682"
     CDS             7259..7765
                     /locus_tag="ACG8ER_001682"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5050260.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7933..9846
                     /locus_tag="ACG8ER_001683"
     CDS             7933..9846
                     /locus_tag="ACG8ER_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5050261.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9851..10852
                     /locus_tag="ACG8ER_001684"
     CDS             9851..10852
                     /locus_tag="ACG8ER_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5050262.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11054..11260)
                     /locus_tag="ACG8ER_001685"
     CDS             complement(11054..11260)
                     /locus_tag="ACG8ER_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5050263.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11906..12754)
                     /locus_tag="ACG8ER_001686"
     CDS             complement(11906..12754)
                     /locus_tag="ACG8ER_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5050264.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12918..13820
                     /gene="gnd"
                     /locus_tag="ACG8ER_001687"
     CDS             12918..13820
                     /gene="gnd"
                     /locus_tag="ACG8ER_001687"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS5050265.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13822..14823
                     /locus_tag="ACG8ER_001688"
     CDS             13822..14823
                     /locus_tag="ACG8ER_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS5050266.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14820..15104
                     /locus_tag="ACG8ER_001689"
     CDS             14820..15104
                     /locus_tag="ACG8ER_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS5050267.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15137..15934
                     /locus_tag="ACG8ER_001690"
     CDS             15137..15934
                     /locus_tag="ACG8ER_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5050268.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15948..16781
                     /locus_tag="ACG8ER_001691"
     CDS             15948..16781
                     /locus_tag="ACG8ER_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5050269.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16799..17656
                     /locus_tag="ACG8ER_001692"
     CDS             16799..17656
                     /locus_tag="ACG8ER_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5050270.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17678..18187
                     /locus_tag="ACG8ER_001693"
     CDS             17678..18187
                     /locus_tag="ACG8ER_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5050271.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18224..18625
                     /locus_tag="ACG8ER_001694"
     CDS             18224..18625
                     /locus_tag="ACG8ER_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050272.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18737..19090
                     /locus_tag="ACG8ER_001695"
     CDS             18737..19090
                     /locus_tag="ACG8ER_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050273.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVEADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19124..19567
                     /locus_tag="ACG8ER_001696"
     CDS             19124..19567
                     /locus_tag="ACG8ER_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5050274.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19584..20669
                     /gene="uxuA"
                     /locus_tag="ACG8ER_001697"
     CDS             19584..20669
                     /gene="uxuA"
                     /locus_tag="ACG8ER_001697"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5050275.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20662..21375
                     /locus_tag="ACG8ER_001698"
     CDS             20662..21375
                     /locus_tag="ACG8ER_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5050276.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21582..21731)
                     /locus_tag="ACG8ER_001699"
     CDS             complement(21582..21731)
                     /locus_tag="ACG8ER_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050277.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22127..22327
                     /locus_tag="ACG8ER_001700"
     CDS             22127..22327
                     /locus_tag="ACG8ER_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5050278.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7733 a         3449 c         4623 g         6624 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tagcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
     1861 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
     1921 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
     1981 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
     2041 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
     2101 atgaatctct ggattggttg aatcaaaatt catttttcca tcagtttcct ttaatgtttt
     2161 attttttcca taagcttgaa caagcttgga gaaattttct ccttcatttg cttctttttt
     2221 tgctttatct gctccttctt tagtcgaaaa tagaatgatc tgtgtactaa cttctggata
     2281 gaattctttc caagcagtgt ctagatcttt atctgttaat tttacattgg ctttcaaacc
     2341 gtattggaaa gccagcatct gtttaaagag tttcttatat gtctcttctg tttggccaac
     2401 tgcttttaat tgttccttgt agtttggacc gagttttttg atttgttcat tatattcttt
     2461 tgttacttcc tttgaagaaa tctcttttcc gtatacatca tcaaatgctt tgaagacaat
     2521 catttttttt aatagtgttt gattatttga tttaactttt gggttatcat aaaaatcctg
     2581 aactgtaagt gttccacctt taaacgttgc aatcgtttca ttggacttgc tacaacctga
     2641 gaatagtaaa attgcacatg ctgaaaggca cataattagt agttgttttt tcaaaaaatt
     2701 tcccatcctt tccttattaa aaacagtcta acagaagaat tagggagaca gtctttcatt
     2761 ttcgtaaggt tgctgtagat attcttagtt cgtaaaaatc tattctctct atgatggaga
     2821 acatttacat agttttcacg agacaagaaa gaaaagggtt gttaaactta atataaagga
     2881 gggatgaaag atgatcagga ttattcggtt gattggatct gttttaatag tcttagttgg
     2941 ttttgtttta ggaattcttg tcaataatgc acttgcggat atgtttattg gaaatagtga
     3001 atggattgga gcaattgccg gtacggtcgg acatttagct gtttttttac tagctttatt
     3061 tcttgctagt aaggtcgaag gaaaaagaat agaagattat ggtgtttctg ttcgaggaaa
     3121 agactggggg tatttagcgg gaggtctcgt tgttggaatt ggagtgtttc tgcttattac
     3181 tagtccatta tatttgacgg gggcgtatcg gttagattca ggaaacgcaa acattgtgcc
     3241 gctgataacg agttttattt tatttattgc agtgggggca tcagaagagc ttttattccg
     3301 tggctttttt cagcatcaga tgcttagatt tggtccgtta atagcaatga ttggatctgc
     3361 tgcattattt gctcttttgc acggattgaa tcctaacatg acatttctgg cagttttcaa
     3421 tatcttttta gcaggttgct ttttcagcgc acttatctac agtacaggaa gtttatttac
     3481 ggctattggt gctcatatta cttggaatcg gacacaagga gctattttgg gtataccagt
     3541 cagcggaacg caagaacaag gttttttctc cactctgatc caatcgcaaa atacaataat
     3601 tactggggga aactttgggg ctgaagcatc tatatcaact acattattct tgttagtctt
     3661 gattgttggt ttgttactct atgcatataa aacaaagcgg atgaagaaat acactgctgt
     3721 ttatcaataa aagatgaaga aagcaaggct gatatcggtc ttgctttttt aagtctgtca
     3781 ttgattaaac gaaaaagtaa acaaattcaa gcgaatacac gacatctttt gttagatttt
     3841 cacaactgac tgtcgtgttc tcatcatttt cattttatat aattccttta tcaaatagaa
     3901 ggtaattata tggagagtga tagaaatgaa gaaaacagcg accgtattat ttgttattct
     3961 tgggctgagt ataattgcag caatgattgg aatcagtact tctgcaaatg aacgggcaaa
     4021 tgagaatctt tcatttactg tgaaaactga tcggattgtt tatgacatga cacaacaagt
     4081 cattactatt ccagtcaaac caaacaaaag cgtgaatgct agtgatgtac atgctgttct
     4141 aacatatggt tgggatggta atggaagttc agaaaaagtt attggggaag tttatttaaa
     4201 agacgtacaa tggaccgctg gtattgagta tacaataatg atttctgcag aactatcgat
     4261 tgatgagatc aagagtaaag ataaagttga tttgattgtt ttttatgatg gacagatgac
     4321 gatcacggag aatttgaaac caagcagttg gacagttgtt ggaccgccaa gcagtacgga
     4381 tagcacaagt agtgaaagca gtacagaaaa cacgagcggt gaaagcagta cagagagtac
     4441 gagcagtgaa agtagcacag aaaacacgag cagcgaaagc agtacggaga gtacgagtag
     4501 tgaaagtagc acaggaagca cgagcagcga aagcagcaca ggaaaagtaa ataatgaact
     4561 tgatttggta cctatcatac caactaagga acaaaagaga aatgcaggga gcaacctaaa
     4621 aagtgtgcta aaacagcctg attttgtttc tcgtggaatt gatcatcaag aaaaagcgaa
     4681 agaaacagat aatagaaaaa gcgatttacc agaaactggt agtagaacct tgaacaagct
     4741 aattacatgg atgggtgtgt tactcatttt gattgtagga gcaagctatt ttcgttctct
     4801 cttccatcga gtgaaataaa gaataaaagc aacagatcta aaattgaata aaacgataga
     4861 tttttgagtg aatatttcat tcgagagtgc tgtattgcct ggcgaagttg tagctttgcc
     4921 agatctattt ttgtttacta acatatttga gaaacaggaa ataacaggaa cacaaatgga
     4981 aaataagagc aagaataatt gacaatgcac actgcgcatg gtagtatctc tgaaaaatca
     5041 gtaacgaatc tatttgtacg ttattcatgt agctgtcgga ataagtctaa ataatagata
     5101 aaaaggagta tgtactttgt ttgaagaatt tattgatatc aatgaaaggc aagtatatca
     5161 atttttgaac tattgttatg aaagagacga gaaattatat gtggtgaagg atatcgcttt
     5221 agatctaaac tacaccctgg ctaagatgaa ttcggtgatt caacaagcag aatcgttttg
     5281 cgaaaggtat ccagaatata aattatcttt tctttcagaa aacaagatga taaaagtcga
     5341 gttttcttct cagtttttac tgagtaaagt ttattctata cttttagagg gaacgattgg
     5401 ttatatttta ctggattcac tttacaaagg tacttatcaa tccttagaaa atcttagtca
     5461 aaaaattata taagtttgcg tacgattcaa agaaaattaa gagaacttaa tactattttg
     5521 aaaaactatc atttatcttg cagcttgaaa aaaggtaatc cgatagaagg agaagaatac
     5581 cgtatccgat acttttttca cttgatgtat tggcagtcat tcgacgaaaa tcattctgat
     5641 atattatcaa gtataaaaaa taaacgtcaa ctattagaaa aatttgcgaa acatgcgcca
     5701 tatacgcgta gtatagatag aaagaaatgg atcagcctac taagaatttc gttgcaacga
     5761 atgaaacaag gccactcgat tacgtatgtc ccagaagaga tgaacaaatt tgtgcatcca
     5821 ttgataacaa aagaagagtt tacgcattac atactagagc ctttttttga agcaaacttc
     5881 tggtcgatga atcacatcac tccaacagag caagcttacc tttattttat gtttggtgta
     5941 atgaacacct atttggaaga agatttgttg gatactccag ataaagtaca agaaagttgg
     6001 ggctggattg aatcgtctat tgtagaaaat tttgaaaaaa acttcaagat ttgttttaca
     6061 acttcagaaa aaaattatct atatgtcaat ttggcaatga tccatttgta ttcaagggtt
     6121 tttgggacaa aaaagaaggt ggatgctttt ggaaaatcag cagaagacag agattttcag
     6181 catatatttc cagtgatgta tccaatcatg caacagtttt ttagtggggt agctcgcaga
     6241 attccagagc ttaatactta ttatgaaaag aacagacggt tgatttttca atactgtatg
     6301 ttagtcaggg atatttttat aaaacacgaa caacctgtca tattctatat tcaatcaaag
     6361 tatggtaaaa cacaggaatt atgggcaaag aaaaggatat tatctatgac tgaacgccca
     6421 ataaaatata gtgcatccgc tgatcagtta cctgatattg tcattagcga ttatcctatc
     6481 gacaaaaagg catatgatca aacgaataca cagatatttt actggaatgg acaacctaca
     6541 aaaaatgatt gggcgcgtct gaaagctgaa atcataaaaa tcaggaatga gaaaaataga
     6601 tccatattgg aaaaattgga tttcgttaat taagaaaagc agattgttta tgccaaaatt
     6661 aagacattgc aaaaagatta ttttctttaa aatcgacaag atgaccagaa agcaagagtt
     6721 cgttgagctc ttgctttctt ttagttttag aaaggaacat cctgatttta tcttcgctaa
     6781 atccagtcaa aagttttgca gattggtcgt agataatcgg ttttttcaat aaagaagggg
     6841 atgtacgaat ctcaatatat agttcactca aactgagttc atctattctg tgaacaagat
     6901 ttttataact aatagaatga gtagagagca tttcggtcac gccgttgttt gtcaatttta
     6961 gtaagtggat aaattcattt ttggttagtg gttgacgaat taagttctgt tcgataaacg
     7021 gtatatcatg gacgataagc caacgttttg cgcgtcttga agacgagcag ccagaacctg
     7081 tatacaattt aatcatcata cattcctcca atagctaagt atttatgaaa taataacatg
     7141 aagaaatagt cgaaaagtgc catttagagg tgtaaaataa gaaaatgttg tcacgtataa
     7201 tggaactagc tagataagaa atactattaa atcaagaaaa aagaaagaag gcacaagtat
     7261 gtcaaacaca gccgacgcaa tagtagtgat cgatttacaa aaaggggtat gttttgattc
     7321 ttcgccgata cacgaattag aaacagtgac agcattagtg aataaaagaa tcaaaacata
     7381 cgaaacgatt gggaaaccag ttattttcgt tcaacacgaa gatgctcaat tagtgccaca
     7441 aacaaaacca tgggagctat tgtcggaatt atctattcct gagcacagtt attttgtgag
     7501 aaaaacccat gcgaattcat tttataagac taatttgagt gttttattaa ctgaattgga
     7561 ctgtcattcg atcgaattat gtggggcaca agcagagtat tgtatagata cgactgtgaa
     7621 gtttgctcat ggattaggat accagcttat gatgtgtaga ggagcaacga caacttatga
     7681 taatcctttt atgaaagcaa aggaaacaat cgctttttac gagaaaattt gggataaacg
     7741 atttctgaca tttttggagc aataagttgt ttcttcgttt aaaaaaagca gaatggatac
     7801 tggtttatcc attctgcttt attacgtaaa atgagaaaat aatcctgttc tatttttatg
     7861 ggataaacca tttatttttt gtataaaaaa tgtttgcgtt tacatataat tctcctagaa
     7921 acataaaaag gagtgaatag cttgagaaca aaacaagtat taaagggagc gttggtcatc
     7981 ttatttgctg taagtacaag cgtcccgcaa tggacagtat atggagagag catttcttcc
     8041 gaatcggaat ctcgggaggt agaaaccaca gaaactactg atgctggaaa tgaaacgaaa
     8101 tcgagtactt cacaaacaga aacaaatact gaaacaacgg ttgagacatc tgttactagc
     8161 acagatcaag aaacgaccgc taccacagat aaccaacaaa cagaagaagc aacagctaca
     8221 aaaatgtcaa acgacgaatc ctcttctgct aaagtaagtg acaaagagaa tgggacagaa
     8281 aatgatgata tcggtaaagg tgaaaatttc aatggtaaag aatatttgaa ttacttcggt
     8341 aaacagatga cagaaatact taatggacca acagaagaca tcaagtcaaa attaatagag
     8401 ttattagtca ctgagcttgc tgttaatgat tatggtgtag ataaagagat tatcaaacat
     8461 ctgattgcta cttatctatt gtacgatatc gcttttgcaa aaaacagtgt gttgatgtcg
     8521 atgcttggag gtacagatga tgggacaacg cctacagaaa acacaggctt ctatccaaat
     8581 acaaaacaac ttcatcaaac tgtactaaac aaacaagatg gcaaaatgat ggatgtatat
     8641 gcatattatg tcgatcaagg ttcagataaa acagtcatga tccatggcgg atttcgaggg
     8701 aattggaata acgggatcgt tacagaagaa tacaatgatt tttataaggc cgggtataat
     8761 ttattatttg ttgattccag agcgactggt aatagcggag gcgattatgt gacctatgga
     8821 caatatgagt cagatgacgt cctttactgg ataaatcaag aagttcgaga gcggccatct
     8881 caaaagattt tactttatgg tggttcgatg ggagcagcta cgatgatgtc tgtattagcg
     8941 aaagatattc ctgtcaatgt caaaggaatt attgaaaatt gcggatttgc atcaattgat
     9001 gaacaactac gttttacgta cagccagaca gtagcccccg cactaccgga tgccataaaa
     9061 aatcagttag acatcattgg ggatcaagag catgaagatt tatttatggg ccttttgaaa
     9121 caatactatt ttgatcagga gatgcatttg gatacaaaag ctgctctacc tacgattgga
     9181 atgtcagatt cattgcctaa attgattatc catggaacag cagatgatgt agtacctgtg
     9241 tctaacgcgc aaaaattata tgagttatca ggagggtata aagatctttt attagtagaa
     9301 ggagcagggc atggaaaggc acaggaagtt gaccatgctg cctacacgaa acatgtaacg
     9361 gactttttga aagtagtgtt ccatgatcag ataaacgtaa agtatgtgga tgaaaacaat
     9421 aaaagtctac tcaaagacca ggacgaaatc cagctttacg gagcttatgg agaaaattat
     9481 atgacagagc aaaagacatt cgaaggatac gaattagcaa atgttgaagg accgactgaa
     9541 gggattttta atgagacgat accaactatt attttctatt acaaaaaaat cccagtagct
     9601 ccaccaaaaa agcaagatcc gacagagaat gcagacaata agggaaagaa tgaacctcga
     9661 gaggatcaat caagtgatga tacgcaaccg attcagatga cagtgaaagt acaaaaaaag
     9721 acagatgcaa aaaaaagaat gttgccgaaa actggagaaa agaaacattc gcttctcatt
     9781 tattcaggtg gatttttatc tgttagcacg attatcacat ggatttggcg aagaaagaga
     9841 gggtaaatga atgaaatggt tcaagcgaat agtgattgca gtaccagtta tcatcgtagt
     9901 tattttagtc actgtatttt tgattaatca aatcacacca aagccgatta gcttgcttgt
     9961 tagaaaacaa tttgacacaa gtgaaaaaga acaagtatat gcccgaccag atgatttcca
    10021 gcaaaaaatc gaacaaatcg atataaaaaa agaaatatcc tatccttcaa agtacaaaaa
    10081 taataatatg gatatctaca caccgaaagt ggaaaagaaa aaattaccga ttcttttctg
    10141 gatgcatgga ggcgcatatg taggagggga taaaaatgat tgtcgtgatt atttggaata
    10201 tctatgttca gacacgcagc aaatcatcgt aaatatagat tatgaacgat caccggaagc
    10261 aaaacatcct acaccagtta tccaattgaa cgaagccatt caagcagcta aaaaagagat
    10321 aaaagatcaa gcagattggt cgaatatttc gattggtgga aattcggcag gtgcacaaat
    10381 tagtggagaa tatttgttag cacttcaaaa tgaagaaatt aaaaaagctg atcatcttga
    10441 tccgactttg gagaacaagc agattacgaa gtttatttct ttgtctggtc tgttggatcc
    10501 ttctgcactt actcaagtaa gtgataaaat ctctagcttt ctttatgaaa aatgtggatg
    10561 ggcttacttt gataataagg actttgaaca atcagagaag gtcaaaagtt tagccttggt
    10621 cagacatgcg gatcgttgga cgcaaaacac attcttaacg gatggaaata caaatacgtt
    10681 tacgaaacaa atggaagaag taacttctgt atttgagaaa gcgggggtga aagtcacaaa
    10741 agtagattat gcggagaaaa atgctgtttt gaaccatgaa tatcagtttg atttcagcaa
    10801 taaacaagcg caagaaacat atcaaaagtt agtaatgttt ttcaaggagt aaagtcttat
    10861 gatgcaaaag aggttgtaat ctgtcactta gctccaagtc acaaggagca atttcaataa
    10921 acaagctttt catggtttat gaatattgta acttgtgact gaggagttag agatggcaag
    10981 gggcaccttt tattcgaaat ttgggaatat cacacgatta ttgtcacgat ctcttttacg
    11041 taaattttat aagtcagatt tgttgttttg atcgtttaaa aaaagcaaca aatccgaagc
    11101 ctgcggaggc aagaagggac aaaaagccga gtggtaagca aaagaatgct ggttccacta
    11161 atattccgtt gttattgata taaggctggg aacaaagatg aatagcaaat aaaatcaatg
    11221 cactagcgaa aaaaagcaaa gaaatatgga tagctttcat aagcagcacc tcctttttta
    11281 taattgtatg ataaatagat taagaatcta taaatgtgac aagtttgtta ggatgtgaat
    11341 aaaatgatca tctagcgaac ttgaaacaaa gatggctaat gataagcaat taatgattca
    11401 gcaatcagat aggtacaaag tttaccatca tacgtctaga ccaataatag aaaatatggg
    11461 aatataatag aaaagattta ggataccccc aaaagaattt ccccaaaaat tcgatgtatc
    11521 ctaaactttt ttattttaca taacaatcat atcaactgtg aaataaaatt acaaatgata
    11581 acctaaaatg aggccaaaag ccagtgttat caaggaaata cttaattcac caaaattttt
    11641 tttaattttt tagaaaataa gaaacaaaaa taataaaaaa aagaaaagat aaatttttta
    11701 ctactgtata ctaactttgt tatcgcatct aacaagtaac tcgaataata ccaatacaac
    11761 cttctccaaa gatgcgatga catacgttct aacccaccga ctattgatgt tctgtaccac
    11821 ccttgcagaa ccaaaaaagc cactaccaaa tatttggtag tggctttttt tgttttatgg
    11881 atttttcgct ttttctgtta tggtgctatt ctttgatttc agaaatagta tgtcgaactt
    11941 tagcgataga acgacgtgat tctttttcgt tgtaaaacat caaaatcaca tatagagagt
    12001 ccaaaatgct tagttgagaa atacgggaag ctaaagcttc agatcggaat tctgtttctt
    12061 cagaaataga aataaaaacg acatcgccaa gtttagcaag ggaagaatta gcttggctag
    12121 taataacgat tgtttttgct ccagtttttc ttacggtttc agcaatatgg attgtttctt
    12181 ttgattgccc tgtgtgagaa atcaaaacag cacagtcttt ttccgtcaat aaagaagctt
    12241 ccatcaattg aatatggtaa tccgtgctgt gagaagttgc gataggggaa cgcaaaaatt
    12301 tatgataagc gtcagttgct acaatctctg aaccgccaac accgaaaaaa tagacacgtt
    12361 tagattcatt gataatagta gccgcttttt ttagatcctt ttgttgtaac aacttttttg
    12421 tgtcatttaa agttgaaata ctagactcga acaccttttg cgccatagtg agctcagtat
    12481 ctgttttctg aatattttcg tggatagaaa tagcagatag atcattttct tggatcaaca
    12541 tggccatttt aaaatcttta aatccattat aaccaagttt ttttgtaaat tgaaaaaaag
    12601 tagagtcagc aatgcccagt tcacttgcta aattgctaat aggactatga cttactttgc
    12661 ttggattttc taaaatataa tcggcgatag cctgttcttt tgggctgaag tcgttgtaca
    12721 ttgttttgat ttgtagtctt atggagacgc tcatgagaat tcctcatttt cataataaga
    12781 ttatcatttt ttccgttctc attaatgtta aagatacaag gagagtagga aaaaatcaaa
    12841 caaaaaatat ttttgtaaaa tctatttaca aaaaaatttt tttgtataat gagggtataa
    12901 gaaatggagg cgctatcatg gaaattggtg taattggttt gggaaaaatg ggattgaatt
    12961 tagctttaaa cttaaaagag caccatcaca aagtacaagg actcgacatt tcaagtacag
    13021 ctgttagtca ggcaagagaa aatgacattc ctgcttatca agagatctct gaatttctag
    13081 ctacttttga gcagaaaaaa atcatctggt taatgcttcc tgctggagag ataaccgaaa
    13141 acatgttaag aaaacttttt ccaaaactcg gaccaggaga cattattatt gaaggcggaa
    13201 actcttatta taaagattca atccgtcgag cagaagagtt tcagaaaaat gaaatcggtt
    13261 acttggattg tgggacttct ggaggaatcg aaggagcacg acatcaagcc tgcttaatga
    13321 ttggaggaga taaagaaact tttctatccg tggaacaatt attcaaagat gtagcaattg
    13381 aaagaggata tctgtatgct ggtgcatccg gaagcggaca cttcttgaaa atgatccata
    13441 atggaatcga atatggaatg atgcaagcaa tcggtgaagg attccaactt gtacaaaaaa
    13501 gtgagtatgc ttttgacttg gaacaagtcg cacgtgtttg gaatcatggt tcggtgattc
    13561 gcggctggct gatggaaatc atggaagatc agttaaacga acatcccaat ttaagtaatt
    13621 atcggggaat cgtttctgca tcaggtgaag caaaacggac ggttgaaaca gcgctagaaa
    13681 tggctgttcc agttccagta attgcaacta gtttgttcat gaggaatctt tcacaggaag
    13741 aagatagttt ttcagcaaaa gtagtttcag cactaagaaa tgggtttggc ggacatgaaa
    13801 tagttgaaaa gggtgagtaa aatgccagat tatcatatag caatcgttaa ttccagcagt
    13861 ttcggaagag tctttccaca ccacttggaa agattaaagt cagttggaga cgttcatttc
    13921 tttactgttg atcaagagat tccaggaaaa gaactagcgg agttacttaa aggattcaat
    13981 atcattattg caagtgttac gccatttttt cgacgagatt tttttgaaaa caaagatgaa
    14041 ctgctattga tttcccgaca tggtattggt tacaacaata ttgatttaaa agcagcaaaa
    14101 gaacaccaga caatcgtatc tatcatacct gctttagttg aacgaaatgc tgtagcagaa
    14161 aataatataa ctaatttgtt gtccatgctt cgacgcacga cacaagcaca agcaagagtc
    14221 aaggacgatc gatgggaaga tcgggcggag ttcgtcggta ggacgctgtt taacaaaaca
    14281 gttggtgtga ttggtgcagg aaataccgga agcggagtaa tcgaaatttt gcgtaacggt
    14341 tttcgctgtg atgttctagc ttatgatcct tacaaatcag atttatacat cgaaagcttt
    14401 ggtgcaaaaa aagtagaact tcaagaatta ctggaacaat cagatatcat ttgtttatgt
    14461 gcgaatttga cagaagagaa ctatcacatg atttctgata aagaagtcga tcagatgaaa
    14521 gaaaatgttt atctatctaa tagtgcaaga ggcgctctag ttgatgaaga agcgatgatt
    14581 aatggtctga aaagcggaaa gatcgcagga tatgccacag atgttttaga agaagaaccg
    14641 ggacgtcaag atcatccgta tttagcgttt gaacaagtag tgattacgcc acatatatcg
    14701 gcctacacaa gggaatgttt agaagcaatg ggtgaaaaat gtgtcagaga tgtcgaacag
    14761 atcagtcagc accttcttcc tgaacgttct gtccagacaa agagcaacta tataaaagga
    14821 tgatgaagaa aatggtgaac ggtttaaaag tgaaaacagg ccctcaattt tatttatatg
    14881 aagaaggtgg tatttcaaaa gtttctgatc tcttgaaatc atacggcgcg aaaagagtac
    14941 tggtcaccca cggaacggta tcatgggaaa aggcgctacc taaattagta tttttgaatg
    15001 acgaaacgat tcaatttttt tatcatcgtt attctggaga atgtagttat gctgaagcca
    15061 gacgaatagc tacaatcatc aaaaaaatga aatcgatttc gtagttggcg ttggcggagg
    15121 aaaactgacc gatctagtgg gttatagtgc caatcttgca agaatcgatt tcggagtcat
    15181 ccctacttta gctagtaatt gtgctccatg gacacctctt gctgtcatgt ataaggagaa
    15241 tggacaagca gaagggaaaa cagaacactt ttacagacaa gctgcttttt ttataacaga
    15301 tccccaatta gtgattgatt cccctgttcc atatttcatt gccgggttag cagatacact
    15361 agcaaaatgg tatgaatctg agctcatctt agaacaagat tttttgcaag atgaatcatt
    15421 tttgaagcta gcacaagata ctgcgaagat ctgcaaagaa gagattttga atcattctgc
    15481 taaagcaatc caagatatgg cacaaagaaa actgacatcg gagttcaaac agctttcaga
    15541 gattatcttt gctgttgctg gtttagttgg aggattagga gataaatacg caagaaatgt
    15601 ggcagcacat gctatgcatg acgctatcag taaatatatt ccggaaagcc atcgttttct
    15661 acatggagaa aaagtagcgt atgggatttt ctatcaactg gcactagaag gtcgctggga
    15721 aaaaatagac gagttatatc ctttgtatcg ggaattagaa ctgccggttt ctctagaaca
    15781 aatgggatta tttccgaaag ataaacagat aatagataaa atggctgaat ttattgattc
    15841 gaaagagaaa gtgcatttga ttcctataga ggtaacacag aaatcactga tacagtcaat
    15901 gatggaactg gaaggttata tcagaaagat ctgaaggagg aggacacatg gaaacattaa
    15961 gtgtagtaca aagtttgttg attgctttat gggtagccgc ggttatgtcc cgctggttag
    16021 gtggaggagc cacgttgacc ttacgttttt ctcctttgat gactggatta gtcgtcgggt
    16081 tgatcatggg ggaagttgca caagcgatga ttgttacagc cgctttgcaa atgatttata
    16141 tgggggtttt ttctccagga ggatcaatgc cagctgaacc atctattgca gcagctatcg
    16201 cagtaccagt cgcattatta ggggatctgt cgccagaagc agcgattgcc gttgcggtgc
    16261 cagtcggatt gctcggaagt tacttgtatc agtttcgttt tttcatcaat acttttttag
    16321 gtaaatatac agacaaagct gtagaagatt tgaatacaaa agctatggcc cgatcaatta
    16381 ttctttatcc aacggttgcc tcatttttat tattcgttcc gcttgttttc attgctttat
    16441 atttaggggc gccattgatc gctgatgtaa tcaaagcttt agagggaaca gtcgttatcc
    16501 acattcttga agtagtaggt ggagggttag cggcaatcgg tatcgcgaca actgtatacg
    16561 ttatcggtcg aaaagattat ctggtctttt tcttccttgc ttattttatg agtgttgttt
    16621 tgaaatcttt agatattacg atggttactt atgccatttt tgggattatt atcgctttga
    16681 tctttgtcca gtcgcaaaaa gggaaacaag tacagcctgc cacaagcaat aacaattcag
    16741 catcttttga tgacgaagat gactatgatg acggatttta atttaggagg aatgggaaat
    16801 ggctgaaata atggataatc aaacaggtgc tcctgaagaa atcacgaaaa aagatgtaac
    16861 aaaagcctat ctgagatggc attttgcaaa cgaaattcct cactcttttg aacgttactt
    16921 ggcgccatca ctactatacg gaatgatgcc aatcttgaaa aaactttata aagatgaaga
    16981 ctctttaaaa gcagcatata aacggcagtt actgttcttc aacactcaat taagctgggg
    17041 tggcggtgtg attactggcc taatgtcttc tatggaacag caaagagcag aagaagagta
    17101 tgaagaaaaa gagatcttga tgcaggatga cttgatgtat aacacgaaag cgggattgat
    17161 gggggcacta gcaggaatcg gagatgcaat cgattcagga accgtccaat atatttttat
    17221 cgcaatcgcg gtaccttggg cacaacaggg aagtccttta ggcgcacttt ttccttttat
    17281 ctgctttggg ttgtatcagg ttttactagg ggtatttttc gcaagacaag ctttcactat
    17341 gggccgtaat gctacaggcc taaagcaaaa cactggtgtt cagacagcga ttgaaatgct
    17401 atcagtttta gggttgttta tgatgggggt gcttgcggga aactatgtaa aagtagaatc
    17461 ttcattgaaa tttgcgatat ccggacgtga gtttgttatc caggaaattc ttgatcaaat
    17521 cgtgccaggg atattacctt tagctgtcgt aatgggtgtg tacttgttct atacgaaaaa
    17581 aggattgaaa gtcacacaag cattattatg gctgactgga atattaattg tgctagcagg
    17641 aatcggtatt ttataaaaag aagggaacgt gaataagatg ggtgtagtca atttagcgcg
    17701 tgtggatgag cgtttgatcc atgggcaagt aatggtaacg ttatctcaaa aaagtggagt
    17761 gaattccatt tttgttgtag acgaagttgt agcaaaagat aaatttatga gagatcttta
    17821 caaaaatgca ggaagccgta caggacaaaa aacaattgtc attacaccag agaaagctaa
    17881 attctattgg gatgaatatc aatttaaaga atacaattgt attttgatcg caaaaacagt
    17941 ccaaacgatt tacgacttag taaaacacgg aatccccatg gaagaattga atattggagg
    18001 gattgctcaa aaaaatcctg atacagatct tttagtgacg aaatctgttt atttaaataa
    18061 atcagatgct gaaaaattga aagaaatgaa tgaacaatat ggcgtaaaga atatttattt
    18121 tcaagccact ccatctgcac ctaaaacagg attaaaagat gtattgaaca agtttgattt
    18181 gtcataaaca aatgcttaga aaaaagataa agagggcgaa aaaatgaaga agaacgagct
    18241 gtttcgtgat tgggaatttc gttatcgcta tatttaccgt aagcgccgta caaaaaagag
    18301 taaacagcgt tttctttcag cattagtatc agatatttac agtatgagaa cagatgtgac
    18361 agtcattgct tacgatacgc tagcctaccg atcaaaaaat atttatgttg gagatatcga
    18421 aaaagcagag aaagttatct gtacttatta cgatacaccc gttcatgcat taggaagtta
    18481 tttcatgttt gattggaaag accaaaggaa aaaaacaatc tactctatat tattgtcctt
    18541 cattttgttg ttcagtttag gttggtgggg aatgatgata tataacaaaa atcctcatca
    18601 cgtattcgat ctgctatccg tataaacagg tatcactgta ctagcatttg ggagctactt
    18661 tttcttattg ggaaaagcag ctcgcggatg gcctagcaga caaactttta ttcgaaatac
    18721 ttcatccata ttaacgatgc tagaaatgat ccgtacgatt gatgatccga gtgtagcgta
    18781 tgcgtttgtt gatgaaggct gttatggaga aaagggctta gacagcgtga gatcgggtat
    18841 gaaaaaagag gctattcttt tttatctaga ctctgttgag gcagatacac cgctacagtt
    18901 ttcgggaaat tatttttcaa ataaagaaca gtggctgaaa caagttgata agttgaaaga
    18961 aaaaaacgtc aattatattt ttagtgcaag aaaaaagcaa gcgcagttct tctatttgac
    19021 caaaacagat ttacgaggga aaacatttaa ttggcaaaat gccaatcaga ttattgctct
    19081 ttttcgataa gtaaaaagtg caaaacaagg agtgagaaga ctaatgttgg aaatcatgat
    19141 cgttacacat ggtaagttga gcgaaggact gaaagatgcg gcggaagtaa ttgtcgggac
    19201 atcgagccat attgaaacaa tgtgcttaga gtctggggag gacatccaaa cattcggaga
    19261 gaaggtcaaa gaaaaattaa ctatgatcaa cacagcagaa cccatgctag tatttgttga
    19321 tctaattagc gcaagtccgt acaatcaagc gctcttggca gtcaatgaac tgactgaaga
    19381 aaagaaagca gccacttatg tcataggggg cgtgaatctg ccaatgttgt tagaagcaat
    19441 caatcatcag atgcttcaca caccgataga agaagcggtg gaacaaattg tcaaacaagg
    19501 aaaagataat ttaactgttt ggcatgcttc agcggaagaa aaaaaattag atgaggacga
    19561 tttttagaag gaaggctgaa aagatgaagt ggggatttag atggtatggt gaaaaagggg
    19621 attcgatccc attaaaatat gttcgccaaa taccgggaat aaatgggata attggaactt
    19681 tgctgactaa aatgcctgga gatgtttggg agatttcaga aattgaggag ttgaaagcat
    19741 ctgttgagag ccaaggactg gaactgcttg gaattgaaag tgtagcgatc cacgatgcaa
    19801 tcaaggcagg gacagatgaa cgagattact atattgataa ctatatacga acaatcaaaa
    19861 acttgtctcg atgtggggtt cgattgattt gttatagctt caaaccaatt tttggatggg
    19921 cgaaaactga tttgtttaat gaaaacaacg atggaagcta ttctctttta tacgatcaga
    19981 aaatcgtcga tcagatggag ccaggagaaa tgtatcaatt gatacacagc cagtcaaagg
    20041 gattcaaatt gccgggttgg gaagaagaac ggttgaaaaa attcgctcag ctggaaaaac
    20101 tgtatgaagg aatgaccgaa gaagaattat ttgaaaatct gacttatttt ttagaaaaag
    20161 tgattccagt gtgcgaaaaa gcagatgtaa aaatggctat ccatccagat gatccgccat
    20221 gggaactttt tggttttcct agagtgacga aaaatcttgg cgacttgaag aaaattctaa
    20281 atgcagtaga ttctccatat aatggactaa cgctgtgcac aggatcttta ggggcaaatc
    20341 cggaaaacga tttagttgaa atcatccatg aggtaggttc gcgcatccat tttgtccact
    20401 tcagaaacgt ggagttttta ggagaaagaa agttcaaaga aacggcacat ccctctacag
    20461 aaggctccct tgatatgtat catattatga aagcgttggt gcaagaaggg ttcgacggtc
    20521 ctatacgccc tgatcatggt agaaccgtgt ggggtgaagt ggctatgcct ggatatggcc
    20581 tgtatgatcg tgcaatggga ctcacttaca tgcaaggtct atatgaggcg ataagtaagg
    20641 aaaaaccact gggagagcat tatgaataaa atcaagctct tcatctttga tatggatggc
    20701 cttttattag aaactggacg tttagcttat cgtgcttatg taaaatcagc acaaaagtat
    20761 gattatgaaa tgcgtaagga agtctattat ttactaactg gccaaacaga aatggcgata
    20821 agagaacaaa tgggcatttt gtacggagaa gatgttccat atatcaaatg gcgagaagca
    20881 atcaatcaat ataaagaaaa aatcgtgaaa gaagataaga gagtatatac gaaaaaagga
    20941 gcagaagaaa ttttatcttt tgcaaaagaa agaggaatcc atacaatcgt ggcttcgtct
    21001 aatacacggg taaaagtaga aatgtattta aaaatggaaa atctatatgc cttgtttgat
    21061 caaatcattt caggggatga tgtaaaaaaa ggaaagccgg aaccagagat ctttttgaaa
    21121 gcttgttcaa aaatgaatat tcctccaagt gaagcacttg tatttgaaga ctcgattgca
    21181 gggatcgaag cagcaaggcg agcgggcatt ttgtcttttc tcatcaaaga tcatttgata
    21241 gggttgcccg atcataatgg gaagcataag ttgaaagtag atgtaagttt atatgaatcc
    21301 aataaagcag actatgaatt ttatgacctg catcaagcaa aacgattttt agaaagtaaa
    21361 gggttgtatc tctaataaag aggactattt tctagtcctc tttatttatt gtcctgcacg
    21421 actgagggaa cagatgaaaa agaagtaatt ttttattttg aagtatctac cttatttgta
    21481 gtgaacaatt tagaacaaac acaaaacgca tctactgtta tctaccagta gatgcgttta
    21541 aagagaagaa cgagaatcgt ccatatgagg gtttccactc cttatgcaga tttttttaat
    21601 tcttgcactg cttgttcgat cgtaacacct gtcgctacat gattggtatt ttctttgtca
    21661 aaaacgacaa atagattcaa atctgtatcc aaggttactc gatattgtat atctttgtta
    21721 tagaatgtca tagccattct cctttcttta aatacttgct gaaaggcaac gaacgcgttg
    21781 ataacgaaat tccatttata agtataccac caattgaaag gttttcatag ttaagaaatt
    21841 ttaaagtcaa aagttgcaga taaaagcaaa taaacaagaa aacaaaaagt gaatcaatcg
    21901 cggttattca actttttctt cgattcgaag acaatagaag acaatgaatt ttatatttca
    21961 ttgcattatc cttgtgttat tagaaaaagc atgatatatt aaatgagtaa tctattttga
    22021 aacgtaattt gagcgatata tctacataga taaaactcct tttgcctatc aaatatttat
    22081 tgcaacgaaa aaacgtacat tgatacgtat caggaggaat ttatttatga ataacggtac
    22141 agtaaaatgg ttcaacgcag acaaaggttt tggctttatc actggtgaag atggaaatga
    22201 tgtatttgca catttttcag ctatccaagg tgacggcttc aaaactttag aagaaggcca
    22261 agcagtgtct tacgatgttg aagatggtca acgtggccct caagcaacaa acattgttaa
    22321 agcttaataa aaattgactt ttaaccgtct tttcgaaaga aaagggctct ttgtcaaata
    22381 ggactgatag agtgagataa gaacatctaa ggtagaattc cttttctac
//
LOCUS       DAZUMO010000042        21979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000042 DAZUMO010000000
VERSION     DAZUMO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.042"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG8ER_001701"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG8ER_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS5050279.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG8ER_001702"
     CDS             complement(2020..2922)
                     /locus_tag="ACG8ER_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5050280.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG8ER_001703"
     CDS             complement(2919..3611)
                     /locus_tag="ACG8ER_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS5050281.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG8ER_001704"
     CDS             complement(3654..4295)
                     /locus_tag="ACG8ER_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS5050282.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG8ER_001705"
     CDS             complement(4333..4647)
                     /locus_tag="ACG8ER_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050283.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8ER_001706"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8ER_001706"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS5050284.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8ER_001707"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8ER_001707"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS5050285.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG8ER_001708"
     CDS             complement(5984..6916)
                     /locus_tag="ACG8ER_001708"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS5050286.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG8ER_001709"
     CDS             complement(6913..7689)
                     /locus_tag="ACG8ER_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS5050287.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8ER_001710"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8ER_001710"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS5050288.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG8ER_001711"
     CDS             complement(10933..12009)
                     /locus_tag="ACG8ER_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS5050289.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG8ER_001712"
     CDS             complement(12014..13294)
                     /locus_tag="ACG8ER_001712"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS5050290.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG8ER_001713"
     CDS             complement(13313..14239)
                     /locus_tag="ACG8ER_001713"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS5050291.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG8ER_001714"
     CDS             complement(14252..15535)
                     /locus_tag="ACG8ER_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS5050292.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8ER_001715"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8ER_001715"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS5050293.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG8ER_001716"
     CDS             complement(16323..17234)
                     /locus_tag="ACG8ER_001716"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5050294.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8ER_001717"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8ER_001717"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS5050295.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG8ER_001718"
     CDS             complement(17700..18398)
                     /locus_tag="ACG8ER_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5050296.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG8ER_001719"
     CDS             complement(18385..18771)
                     /locus_tag="ACG8ER_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050297.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG8ER_001720"
     CDS             complement(18918..20588)
                     /locus_tag="ACG8ER_001720"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS5050298.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG8ER_001721"
     CDS             20771..21004
                     /locus_tag="ACG8ER_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5050299.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG8ER_001722"
     CDS             complement(21103..21534)
                     /locus_tag="ACG8ER_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS5050300.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..>21979
                     /locus_tag="ACG8ER_001723"
     CDS             21613..>21979
                     /locus_tag="ACG8ER_001723"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS5050301.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNL"
BASE COUNT         6208 a         4914 c         3884 g         6973 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttg
//
LOCUS       DAZUMO010000043        21704 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000043 DAZUMO010000000
VERSION     DAZUMO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.043"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(602..675)
                     /locus_tag="ACG8ER_001724"
     tRNA            complement(602..675)
                     /locus_tag="ACG8ER_001724"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
     gene            complement(865..1890)
                     /locus_tag="ACG8ER_001725"
     CDS             complement(865..1890)
                     /locus_tag="ACG8ER_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS5050302.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            complement(1887..2111)
                     /locus_tag="ACG8ER_001726"
     CDS             complement(1887..2111)
                     /locus_tag="ACG8ER_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5050303.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(2108..2401)
                     /locus_tag="ACG8ER_001727"
     CDS             complement(2108..2401)
                     /locus_tag="ACG8ER_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050304.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            complement(2439..2576)
                     /locus_tag="ACG8ER_001728"
     CDS             complement(2439..2576)
                     /locus_tag="ACG8ER_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5050305.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            complement(2578..3024)
                     /locus_tag="ACG8ER_001729"
     CDS             complement(2578..3024)
                     /locus_tag="ACG8ER_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050306.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(3021..3170)
                     /locus_tag="ACG8ER_001730"
     CDS             complement(3021..3170)
                     /locus_tag="ACG8ER_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050307.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(3187..5313)
                     /locus_tag="ACG8ER_001731"
     CDS             complement(3187..5313)
                     /locus_tag="ACG8ER_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050308.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            complement(5337..7628)
                     /locus_tag="ACG8ER_001732"
     CDS             complement(5337..7628)
                     /locus_tag="ACG8ER_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5050309.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFCSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            complement(7638..8375)
                     /locus_tag="ACG8ER_001733"
     CDS             complement(7638..8375)
                     /locus_tag="ACG8ER_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5050310.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(8426..11857)
                     /locus_tag="ACG8ER_001734"
     CDS             complement(8426..11857)
                     /locus_tag="ACG8ER_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5050311.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGTKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            complement(11874..12056)
                     /locus_tag="ACG8ER_001735"
     CDS             complement(11874..12056)
                     /locus_tag="ACG8ER_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050312.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            complement(12059..12421)
                     /locus_tag="ACG8ER_001736"
     CDS             complement(12059..12421)
                     /locus_tag="ACG8ER_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050313.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            complement(12441..13049)
                     /locus_tag="ACG8ER_001737"
     CDS             complement(12441..13049)
                     /locus_tag="ACG8ER_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5050314.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            complement(13061..13465)
                     /locus_tag="ACG8ER_001738"
     CDS             complement(13061..13465)
                     /locus_tag="ACG8ER_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050315.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            complement(13458..13859)
                     /locus_tag="ACG8ER_001739"
     CDS             complement(13458..13859)
                     /locus_tag="ACG8ER_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050316.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(13849..14202)
                     /locus_tag="ACG8ER_001740"
     CDS             complement(13849..14202)
                     /locus_tag="ACG8ER_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5050317.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(14192..14503)
                     /locus_tag="ACG8ER_001741"
     CDS             complement(14192..14503)
                     /locus_tag="ACG8ER_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050318.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(14500..15375)
                     /locus_tag="ACG8ER_001742"
     CDS             complement(14500..15375)
                     /locus_tag="ACG8ER_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050319.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(15385..16545)
                     /locus_tag="ACG8ER_001743"
     CDS             complement(15385..16545)
                     /locus_tag="ACG8ER_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5050320.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(16545..17231)
                     /locus_tag="ACG8ER_001744"
     CDS             complement(16545..17231)
                     /locus_tag="ACG8ER_001744"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5050321.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(17194..18372)
                     /locus_tag="ACG8ER_001745"
     CDS             complement(17194..18372)
                     /locus_tag="ACG8ER_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5050322.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(18392..20086)
                     /locus_tag="ACG8ER_001746"
     CDS             complement(18392..20086)
                     /locus_tag="ACG8ER_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5050323.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(20064..20378)
                     /locus_tag="ACG8ER_001747"
     CDS             complement(20064..20378)
                     /locus_tag="ACG8ER_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS5050324.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(20481..20762)
                     /locus_tag="ACG8ER_001748"
     CDS             complement(20481..20762)
                     /locus_tag="ACG8ER_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050325.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(20767..21111)
                     /locus_tag="ACG8ER_001749"
     CDS             complement(20767..21111)
                     /locus_tag="ACG8ER_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5050326.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(21137..21304)
                     /locus_tag="ACG8ER_001750"
     CDS             complement(21137..21304)
                     /locus_tag="ACG8ER_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5050327.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
BASE COUNT         6237 a         4542 c         3308 g         7617 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg cccgcatcat
      961 tacatagact ttcttactag gaaaattacg catattgaaa gatacatgat aaccaacatt
     1021 tccagaagta ttataagctt ggtttacatc tggtctataa attccatcag ctcttactcg
     1081 agctaattct ttcccagtat tgtagtccat aatgaaaata tactcgtatt tatagttagc
     1141 aatgtgccat ccagccacat gcaagtttgc gttttcgatt tccccaaact gatcaatgtg
     1201 ggcgtgattt gttccatctg tcagcgtagg attagctgca ccagctcgtg ttggatcaat
     1261 gacaggcttg ttttcagaag ttgttggatt ttcatcggta aatccatgag ctaaatcata
     1321 agcaagcttt tctttgctaa ctcccatttg cgataagtaa ccatatggat ctgtgtggtt
     1381 cccccaaaca taatttgtca cccacaaatg agaaatgatt ccttttgtaa ataaagaagt
     1441 tccttgatca agagtcaatg gaattccata tttttttgca ctatctcttg tatattcaat
     1501 ataagctcga tagttttttt caaacaatgc tttatcatat gtgcgctgta attcaatctg
     1561 tacaggcgca taaggattag cgttaccagc tccccacgaa acatatcctt gctcacccac
     1621 acggtaaaca atcccaccgt caccaataac atctgtagta taagaattgc ttccgttata
     1681 attatttttc atgttggcgg ctacgtttct tgctggtgca tctattccag tttcgtgcaa
     1741 aataattttg ttaggaattg ctaatctgga gtctccttga ttcggcgcta aattatactc
     1801 gtcattaata gtataagcaa acgtattaat gggtaataaa aaaagagccg ttaacaggct
     1861 catcgcagta atagtaattt tctttttcat ttgtttcctc cttcttcgct ttcagccgag
     1921 aacattttgt aggttcgatt tgatacaccc aacacactcc ctaaaaacgc gccaaaacca
     1981 gtaatgatga caacacagat atctgtgtac tgccaattga gcgctttacc aactaacccc
     2041 acgaaagtag ctagtgcggg aataattacc agtgcgaacc attttagtac ttcgaacgtt
     2101 ttattattca ttttcttctc tccctaaata aagttttaat ttgttgcgtg tgttctacca
     2161 atttttctgc atgtgtatct aatctttcat cgtgtttctt tagttcttca tgaatcatca
     2221 atcgatctga tttgctcgat tctaaatctt tagtcagcaa atctaaattg tgacttactt
     2281 ttgaaagagt ctcagtaatc ttcgagaaag atgcagtaat tggttttatt actaataaaa
     2341 tcaaagaaac gatagcggtt attgatcctg ctatcgctcc ccattcccct aaattaatca
     2401 tgtgacaact ccttgaatca aaataaaaag cacatcaatt aagatgcgct ctcttctttg
     2461 ctaatgattt tatctgcttt ttcttcagta atgcacaacg gaacgaaaac cattacttgt
     2521 tcgttagtga aacagcccca atcatacatc attttcacat cgctaaaact aaacatacta
     2581 ctcacctccc tttgaagctg gatttagttg ctctttaatt tctgaaatgt ctttgctatt
     2641 ttgtaacgaa gcaagcatca tttttgaatt gatttgtgct aaactatccg ctttttcttt
     2701 caatgcagta ttttcctgtt taattgctac atcgcttagc atgagtttgg cattgatctg
     2761 ttttaaatcg ccgttctcat tttctaacga ctcatacatt gctttgagat tgtttaaatc
     2821 gttgtgatct agtgcgttcg ctaaaataat ccattgattc aatttaggat caaacatttg
     2881 atcagcgatt gttaacggtt cgccatcagc acgaattcct tcaagcggtg gctgatctgt
     2941 gtaaggaacg gatacaagca tgtcgtccaa tacttttcct gcgtactctc cgccagtacg
     3001 tccatatttc caaatgtttt tcatttattt ccctcccagt aattgaattt cggtttccaa
     3061 tttggaatcg gtggttcgac ttctgtgcat tcttccggta aatgttcttc atcattcaca
     3121 atgatttgct cgaatccgta aggttcaatt ggtctatatg ctgccttcat atcgattcac
     3181 gcttttctaa atcgtgtaag taattgcaaa ggtataatcc gatccataac ttgagtttct
     3241 tcgccattta atggctccat ctgcaccaat agataactga gcactgttca aagtagaacg
     3301 gtctatcgag ccaaccagtt gctcaaaact aattggtggc cgatagcctt ctggaattgt
     3361 tagtatcgtt gaatcatttc caccactgct ttttccgttt aaagccacaa aatatataga
     3421 aactgttttt ccttcacgat aaagctttgc tgatccggta ttcccgtttg taactgttaa
     3481 tgtggtagta gctgtatcat taatgcgttc atcgatctta ttgtctaatt catctatagc
     3541 agtcgcatta gcattcgctt ttgtttgagc atccttagct gtggtgtcta cttcattaat
     3601 tgaagcagtc aactgcgaat taatctccga tactttccca tcggtataat tgtttgcttt
     3661 accagtaatt tcagaaattt taatatctgt ggccaaatta tcttcgacat attctggtgc
     3721 tagatcccaa acataatctt ttggattgtt tgaatcacgc ataccagtac cacgatattt
     3781 atactcacta atattcgggg ttcgagtatt gcctttttct agtttgagcc actcaatttg
     3841 aacagctcct tttgtagctg acgggtattg gtatatttcc agataattag ttaccccact
     3901 atctatatgt tgcttagtta cagtaaaagt taactcccat gtattagcta acccctcagt
     3961 aggcttcatg tctcctactt taacgcggcc agcagcaata tacacggcaa acgattgaac
     4021 tgttagttta gttgctttca ttgatatggt atatgtttgg tcagcgatat agttttctgt
     4081 attgcgtcta ccataaacca tatattcact agttttgtgt ggaaagacaa ctgtagggtc
     4141 agcaatattc tcgcctaaag gaactttacc taaccagtaa gggccatcta gtaagttagg
     4201 gtagtgtgct tttaacttaa cgtcatagct gacactaacc gttcctttat attcactatt
     4261 aagtgagaat atttcaacat aacagtttgt tatgctagtt acattatctt ttgttatacc
     4321 agtaaatgta tgagtgacca ttttatcgcg ttctacattt gctggtatta atgcattaat
     4381 gggcagttct ataccacctt gttcatttaa atatctaagc ctaaccccta tagtattggt
     4441 ctctccagtc caatctgaac cgacactcat agtaacgctt aatgtgtatg tcgtagatgg
     4501 tcgtaaagtg gttataagat acctattatt agcttccgct gtacacggaa tacctaagcc
     4561 aagcgataat atgtcgccgg aattagctgt tctagtttta gtatgagtta taaccctttt
     4621 aacactatct ttaactgtat gtccattacc taacaaaggt ccaacccaag gttctgtaat
     4681 atatggcatt aaattcgggt tctccgaata atcatagtcc ccgaagtcga tgctgttact
     4741 gtacatcact tgtaagttac ctaacttaga aatttcttct ttcagagcat ctaacttgtc
     4801 ttgtagcgtt ttagcttgac cagttaaatc agtaatctgt tgatttaagc tatccactct
     4861 acctttgatt tcagccataa aagcatcaaa agtttcgttg tactttcgaa tcaactcttc
     4921 taattgcgaa acatattcat cggcttggcc ttgcgaaatg tcagacactc ctagtgagaa
     4981 aaaaatgata tcttgcgttg ttaaaatttg attgtctttt ctatattcta cgtagcagtg
     5041 tttataatat cctgcttcac tcataaatgt gccatcaaga gaaaacgtga cttcttcact
     5101 agttacacta gttgcaacat tatctacgta acggttagat ggtgttgttc cttttaaagt
     5161 aaatgttccg ccactcgtat ccatctgcaa gccatttaga aacggtttaa ccgtcaccgt
     5221 aatcccttta tcaccttgac gagccataat agctttggtg tagtttaatt ctttgctgaa
     5281 atctaaagcc aaattatata aactgctagc catttatata cctccttgtc ttcgttttaa
     5341 aaacgttttt ggtcaagcac tgtgctatca tatgctgtat cctcttttaa tctaatatct
     5401 tcatacccta gacggtgtgc cactaaattc catctaacta atacgtttgg cttactagtt
     5461 tcaatgatga aatggtcaat atcttcatga gtaacagcac acaaaactag ttctgtaggt
     5521 gtcacatgtg tcatataccg acttagattt actgtttcag caaacatggg gtcaatatca
     5581 acacgaactt taccattgtc acctgtaacg gcttccccat aatcagcgaa ataatattct
     5641 ggagtttcat aagcgttcaa tagtcgttgt ccataatgtt ctgttggtac agttgagttt
     5701 ttagtacctc taacagtaaa atctttatat acttgtaccg ttgattgttc aaacctagca
     5761 agtttcccat cttcccatga acaaaaaaaa caacctggta acgttagcat accatcacta
     5821 gtaaatttca tagtcctacc agctacctta aattcccatg agttacccgc actaccatta
     5881 atgcttaaag aactaccgtc gccagaagtt acataactag cattgctata cctgaaattg
     5941 ggcgcaccaa aagatagaaa cggtctgtta ttaccattat cccacgtact aaaaaccaag
     6001 ttaccctgtg gatttctaat catgaaacca ccaccagttt tcatggtgta tgatacaata
     6061 ccggcatcag cacttacata atcacgtgct tctagctcca taatatcttt gttaactttt
     6121 tttgagtacc aagtcatttt gccattagca atacttgttc tataatcagc accatcacta
     6181 attaatgtag tacctctaat agtaattcct actatttcac cagccgtaat aaacgaggca
     6241 ttgaatccgc catctaacgt ccatgccgtt tcatatgttc cattaatgcc agttttagaa
     6301 aaaccaatac cagcattgtt gatttgtaaa acattccttg cggtattctt atctggtgtg
     6361 tccataatca aaatacgact aggcgcttct ttaggatcta ataaaacata accaccattt
     6421 tgaccagtaa tcatatcagt ttgatgatct acaatatcat tgagtaaatc actgatttcg
     6481 ccaccgtttt tcaattgatc aatggcatca ttaatcaaat tgctgacatt attctctgtg
     6541 ttttctaaga agtttgtttt gacgtttcct acaactaatt tatcgtatga attggttaga
     6601 acattaaacg tatattccac aattctcgct gacatattca cttttaactg tggatgatac
     6661 acatctactc cgtcacccat cgaaactttt tctagatcaa caaatttttc atagcctctt
     6721 tgatgcctca atggtactaa ttcaatcgaa ccactcactt gtggtttttg tttatctatg
     6781 tttgttttca accagtcttt agcagcttcc cttaatgtgg ctacatcagt cgctttgtct
     6841 ttaaaatcaa caaaagaaac atatccagca ggataatcat ccacgtaatc cgtgaaaata
     6901 acttcttctg gtagagtgat ctcgtcttct ccttctgaag agctgctaat gaatggataa
     6961 actccaacta aaacactttg agcatcaatc tctaagtcaa gaccagttaa gtttttagta
     7021 taaatcgctt tgattttatg atccgtacct agactttttt catgacgtaa tgtgttatta
     7081 tcttttagaa actcaccatg aaatcgatct agaatagatc cctcttttcc accaaagaat
     7141 tctaaaaaat tcgccttttc tatcttcaca ttagcaagcg tatctactaa tgacgagaaa
     7201 gaaaactgcg aagggatagc tggtttcgct aaaacttttg cgttttgcca tgcctgagta
     7261 gcagtgatct tttctgttcc gctgtcatat ttattcaaca ccgattttct tatatcattg
     7321 aaaataggtt cagcttttac ttctatcgta ttgcctatta cagaagtttt tgcataataa
     7381 atccgtagac gctgttttgc tcgattttca tctacataac actgaataat acgtccgtct
     7441 acaatcaaat ctgcattagt tccgtttatt gaataagtac cctgaaatat ctcggctccg
     7501 tttagtttat tgctaacagt agctgttaac cagtctgaca aagcgcctaa accttgcgta
     7561 tcatataaat gttcagctaa attattcgcg tcatttttat cgtaaatagt tattaaatta
     7621 tcgatcatct atttcaccta ccttaacccg ttacgataaa tttgtatttt gctcaaacca
     7681 gtgcaattaa aatgattgat atccacttgc aatgtcggat attgcatggt cttcattttg
     7741 ttggaccgat ctaaaatatc tccgtccgat tgctcttcgt agcaaagcat caaatcacta
     7801 tcaatgacta catcagttcc tgctactaag ccttcgaaac taaacacata atcatttaag
     7861 atgaactggc atgaagtagc tgaaggagtg atgataatct ttggaaaact ttcttctaaa
     7921 ctattattca gcaagttaaa tgactgtggt ttatctacgg ttataggtac atcttcttga
     7981 actcttgcga atggtttcgc agtaatattt acatcgaact ctccccattc aacaatatcg
     8041 ttttctgcat ccccaatatc gatagtctgg ataacgtaat agacgttggg atcgtcagag
     8101 aattctaatt tctttgcata gtttaaccaa tgacgcatga tataaaacga ttgcttgaac
     8161 gcttgatggt cttccacatc ctctaaatag ttatagtgca atgtaaacga catatcttca
     8221 aacgagtaat cttgtactaa gccacctaac ctacctaaaa cagaagtttc aactctctgt
     8281 ctttttggag aaggtatggt tggtctttca gctaaagcca atttatgcaa ataatcagga
     8341 aatccatcga ttatagaatg tatacaatca gtcatttttt cacatccttt ttaatactaa
     8401 aaaaacagga gaaatactct cctgtttaac gccatgccga agcattatca ttttgaattt
     8461 ttgtaatgct atcaatgatt tgttgagttg tttgcttcat agtaacctca tctgcgttac
     8521 catcaattgt gaaattgaat tcgtagttat tcacaggttg aatcgtttgt gccctagatg
     8581 aaactgatgt gctactcaag atacgatcac caatttcttg caaaacagat cttttcaaag
     8641 gtaaaactgc ttcaggtcct gcttcaccga caccgttcat tccacctagt aaagttggtt
     8701 tagtaaagat acctccttta gcatgccatt ttacacgcaa atgggggatt tgacctttta
     8761 gcgggttaaa gctgccttcc atgataaatt ccggtaacgg aatatgtggt atagaaatat
     8821 tcaaattatc aaaaatgcca ctgattttgt ctctaatcca atcaattgga gcgctaacag
     8881 tctttttggt gccttcccat atgttagcaa ttgtactttt aacattattg aatatgtcgg
     8941 aaacaatacc tgttagattg gaccaaccgc ttgaaattgc attttttcca tcgtttactt
     9001 tagagctaat agtgcttgta attccattcc aaagattcaa agcagtattt ttgataccgt
     9061 tccaaattcc gctaatccac gaagatatac tattccaaac actttgaatg gcacttttag
     9121 ctgcgtttat agcattgctt atactactag tcacactatt ccagatattt gatgctgtag
     9181 agctgattga attccaaatt ccacctaacc aactagatac agttgaccaa atattttgaa
     9241 ttactgtagc agctgcttgt accaagctag tgattgtatt cttgatactg ttccaaatac
     9301 tagaagctgt tgcactaatt gaattccaaa tatttgaagc cgtagtacta atagctgtcc
     9361 atataccatt ccaccatgcc actactggat caaatatagt atggaatgta gttacaatcc
     9421 cattccaagc gatacttatc cattgtgtca tagtatccca agtattttta aggaaatcag
     9481 aaataggtgt ccaaacagct tgccaagctg cgcccaataa ctgtccagct acatcaaaaa
     9541 tacccacgat aatattaata ccagcttgaa tcaatgacgt tattaatgtc catggtattt
     9601 gaacaattcc tacaatgtct gcccaaataa tcgaccatac ttctttgact ccgttccaaa
     9661 tatttgaaac ccaatcaacg aatgtttgcc aagtttcttg gactccttgc cagatgttgg
     9721 aagctccttc aactaatccg ctccatagct ctccaaacca atcagaaact ccttgccaaa
     9781 tatcttgaac ccaatctaca aatccagacc aggtttcttt aaccccatcc caaactgatg
     9841 aggctccgtc ttttatactt tcccaagtat cacccaacca atcagtaaat tttttccata
     9901 aaccactaaa ccagtcagta attgcacccc agttcttaat tgccacaata acgcctgcta
     9961 taacagctat aattcctcca attataagca tcatcggacc aaataaatat gacacggcta
    10021 gtattgccgg taacagtatg ccaaatgctg ctgtaagacc acctatagca acaatgaaat
    10081 cctgtactgg ttgtggaaga ttattaaacg catcagccat ctttcctaga aaatcaatta
    10141 ctggttcgag tgcatctatg attgtgttgc ctataggagc taatgaatcc tttaattcag
    10201 ctattttccc gttcaactct tgcaacggag tagtagaatc ttcattcatt ttttgtgcag
    10261 atccactaac atcatcaaat gtatggttaa catcagttaa agattggaca actttcatcg
    10321 cattatcttc gccaagcgca gaccaaattg tcgaagcttt atttaattgg tcgtattgac
    10381 catccatatt gctaaaatct tgaatcatgg aattaataac gtctttttgt gttcctccgc
    10441 catttttcca ctcttcaaaa gcttttctag tactttcact aaacatatcc atgttttgct
    10501 caaatcgacc atctgttaac gatattccca tttccttaac taagtcattg actttatcaa
    10561 ggttataagc acccgcatct aaaccatttt gaagcattcc gaacgtttca tcagctgaat
    10621 atcccatttg actccataat tggctatatt ctgccatatt gtcgcctaat tcgtgcgttt
    10681 tatctaaacc gttttgagta cccgaaacca ttaaatccat tgcatcttga gcactcaagc
    10741 cgaaattgac cattaagcca tttacaccac gtaacgtttc atccatatca gcgcccatgg
    10801 tgttttctag gaccatagct tgttccgtga tattttgtaa atcttgatta tttaaatctc
    10861 ctaaattacg ctttaccaaa atcaatgcat ctgtggactg atctaacgat tctccaaaac
    10921 ctttataata aatgtctctg gctacattcg ttaattcttc agcctcttgt ttagacaaac
    10981 caaaattagc ttgtatctta ctctgggaac tacctacact gttagcagag tccactgctt
    11041 gtttccctaa ttctgtaagc ttatcgccaa tgtcgcttaa aacgtcagaa gcttccatta
    11101 aattattcat atctattttg cttccgatat cgtccaagtt agttgtatct acatttttag
    11161 cagcttgtcc taactcttca aattcacgtt cagcatcatt aagcttcgct tccatctgca
    11221 ttgcttctgt ggatgtagcg ccaaattcag actgtgtagc ttctaactgt cgtcttagga
    11281 tatctatcgt tttctccgca ttttcagatt gttgagaaac atattcttgg gctttcgcta
    11341 atttctcggc ttcagaagct gattgaccag cagttgcttg ccattttttg tattcggatt
    11401 caatcagaga agcactagct tgaacatttt tttgttcgct atccaactgt tgcattgtag
    11461 actcgtacgt ctgtatttcg ccttttgctt gagctagtgc attactcgtt ttatcaattt
    11521 cgtttgacaa acgttgttgc gctgtttgtt gattaatcag ttctctttca agtttctgaa
    11581 cttcggtgga attttctcca taatattttt tcgcattggc taaacgttgg ctagttactt
    11641 caactttttg actttgtaat tcatactgct tttctaaaga agataattta cttcctaact
    11701 tgtctgattc agaaccagtc tgttgtaatt gagcttgttc tagttttaat tctgctctat
    11761 ttttagttaa ttcagcactg atttctttta acgtagattt caatccgtca tcgttagcta
    11821 tgaaagttac ttctgcttct gttctctttt tagccatttt ttacctcctt tctttagttt
    11881 ttctgggatt ggtttattgc atagtttttc catccttcat aagcactctt gttgtaagcc
    11941 atttgcaaaa tgtcatctaa acagatatcg cttaaaacca aatctgaagg catagaaaaa
    12001 acgtcggtca acatcgaata gacatcgacc cacgtttcaa ctaagagctt tggaattttt
    12061 acttttgaag ctttttttcc ttatttgctt tttcaaattc tttttgataa gcatcgcgtg
    12121 cttgtttgaa catcatcaat tgataaatat agctggcagt agccatatca aaatcccatt
    12181 tatcgataaa ttcatcgaat gaaatataat cagtcatgtt cgcttggcgg taagcaatat
    12241 acacagcctt tgcaccttga ataacagaaa tatccatgga tccttttccc acagtcattt
    12301 ttgcaaactc gtctgtgtta aaatctctat tgatcatcaa taatttcttg atattcagtt
    12361 taggttctaa attcaaaatt gttccatcgt ttagttcaat ttttgagtaa tcttcgttca
    12421 tttcgctacc tccgtttttt ttactgtgat tgagtggccg tagttgtcac aactgaagtt
    12481 tttttaatca catcagcaga tagattcgtc atccattgat ctgttaagtc ttcttcaagt
    12541 tctgcaacaa ttgcttcatg ataaaattta ccaaattcat cttgcataac ttttgtttct
    12601 agttctaacg cagctacttc atccgcacca ttttcaatag agaatgttaa tcctgtattc
    12661 gaagtgcatg ctaacatacc aactaacttt ctattttctt cgaagtcatc cacgatctct
    12721 gcagcaagtg agaaatcttc gcctacggaa tcaggaccgt aagagtaaat gcctggttta
    12781 atacgtccat cttgtttcaa cccattgaaa cgtcgataaa cttccatcgg tacatgtgca
    12841 gtaattgtta ccgtcatatt gattggttta gattttgatt ttacttctgt cgctccacat
    12901 tttttaacca ccgtttgcat ttctgtttcg ccatctaatt gtccattaca attcgttgcg
    12961 attgcatttc ctgcgttctt aaaattaaaa gcaattcgtt tgatacttac gttatcgaac
    13021 gttgttacta cagtttttgt tttagccatt gttgttcccc ctatttattt aatttatcga
    13081 attgacgaat cagaagttct gtaattggat caagtgcaag acctaatcct cttctcataa
    13141 attcgtccgg ctgatttctt ttagaagtac ctatccccaa atcaggatat tttaaatact
    13201 caaattttct tgtaggtcta atgatgaaac ccaaattaat gtattgagtc ttaagtggac
    13261 gactattttt tgcgtgttgg tgccctcttc ttaaatctgc ttcagaaaca ggaatttttt
    13321 ctgtaatcct atccactgca atagccgaac cttttgattt caatgcttcg ttaatcagtc
    13381 gttcgctctc gcttgaatag cgttccatcc gcacaagaag ttcatcatgt ccatttattt
    13441 ttagctccca actattttta gccatgacaa tcactcttca ataatcgtct aaacgtaaat
    13501 accaattgat cgatatagcg atcttggttc tctagtttta aatgattggg atccattctc
    13561 tgaaaacgaa tcgaacgatt ttgaatcaat gaaataatat ctagtgagtc tcctgttaaa
    13621 tcttctctat tttctgaata gaaagttaga tatagatttt gacccacgct atattttggc
    13681 tcagtgatca tttctatttc tcctgtttcg agaatgaagt aattaaaatc atcaggtagc
    13741 tcatcctcgc ctacggagtc ttgaaagagt ttgaggccaa aatgctcttc taaggaagtt
    13801 ttgatagcag aaatttgctt atttaaacgt tctttttctt tagaattatc aatcaccata
    13861 ttcacccaca ctttcaagat aaaaatagat ataaaaatta tcgtaatcgg catagataac
    13921 gttgtaacgc atactatcga ttacgataaa atattgatct ttattaaatt tcttggcgat
    13981 tggatgaaat ggagtcttta ctttcttagt taatttcgat cccatcgcat ccatagctgt
    14041 tatatcacta tctctcatgg aaagatttct aaattttaaa gaagtgattt ctgtatcttc
    14101 tacaccaatc ttttttccta gttcatttct tttggtagtt tgcgtcaaaa tctttaacca
    14161 accatcgttg aatgtttctt cgagtctacg attattcgcc attcacatca cctgcaatat
    14221 attcttgtag cgcataatgt tgaatgaaac ctaataactc actagcgaaa ttttgttcaa
    14281 actcatctaa agcacgattc cagtcgtatc tacatctttc gattagcaat ccgtattcta
    14341 agctttcagg agaaaaagaa agtgttgtac tcactttact ttgaagataa acagcatttt
    14401 tagctatcat ctttttaatt gactcatctt cttcgttcca ggtaacgtaa atattatcct
    14461 tcacagctat tagcaattct tcagtcactt gttcaggcgt catctaacca ccgccttaat
    14521 tgctttaaca tatgcgtaag agcatttttt cttgtttaca aatgataaat cttcatcaaa
    14581 aggcgtagaa gtcacgtatc tccctttgaa aaataaatct tcatcgtttg ttgttactcc
    14641 agcattgtgt aagattttta cttctttaac tttttctatt ggatcagtag caaaacaaaa
    14701 gtctaattcc tcgtgaactt taggaccaat attgaaatac atcatgttcc aaagctgtgc
    14761 ccacatctcg gctgtccaga tttgtatatt tgttttttgc cctctaaggt agcgatatag
    14821 ccgattagaa tccagataaa cctttttcca ataattcgct ttaggacggt taataaccca
    14881 ctgtgcgcct cctgaattag tgtttatagt ttccaaagat tctactgtaa catttacaat
    14941 gtttgccata tcttttagaa tattttctcc gttttcacag cttctaatat aatcaagact
    15001 tagataacta cagcagtcgc tacaatacca aacatcatct ttagaaggca atttgcgcaa
    15061 attaattctt ttattgaaaa tgacatccga atcgatatag aaatatcggt cgtcctcacg
    15121 cgaatgatct tcttctaaat atttccacca taaatatggt ttaatcgaag gaatatactc
    15181 tttgtcgtcc cgcagatcat cgtacacatg aacttcaaca ccatattcct tctcaaaaaa
    15241 aataggaatc tgatcatcgt gtctgctgaa aagcaatacg atatctttga ttcctagttt
    15301 cttcagatta gttaaacaaa cttcaagctc ccatttaaac cgattgattg ccggctgaca
    15361 aagaatatac ttcattctga tcacctacgc ttgtgttgta gttgttgtgg ttgttggttt
    15421 tgtagttgta gtagtagttc ccaaagcgct aatatctaat acaatgaaac tatcgttacg
    15481 tttaggttga ccgtttgcat attgtttagc tagataaatg cgttcgtctt caacaaaatg
    15541 gtattcatct gaagcttcaa tttttagtgt agatcctaca cccatgaagt aatctgaggc
    15601 taccccaata actgcttttc cttctggcac agccgttgac tgcaaatctg aaactggtac
    15661 tggcaatact tgtacgtatt ctccattagc agttagtaca gtcttagctg ggaatacttt
    15721 agaccagtaa tcagttggat tcacaattag gaccacatca gaaggattca cattacgata
    15781 aatcggatca ttcacacctt cgatattgaa ttttgatagt cgcgccatca aaccgcccat
    15841 agttacagca tctaaagctg taataggttc tgcttttttt tcagcatatt ctccgctagt
    15901 ttgtttgctc atgtcacgca tcattccgac tggcatatct ttaccagtac catcaacaat
    15961 tgcttgttct aatgcaattc tcaatgattc tactaaaaca gtacggacat aacgatctaa
    16021 ccatactgga cctaaatcaa gcattgcctt acatacagga atataacctg atagcttgaa
    16081 ctgcttcatg ttaattacat caaagccatt atctaaaact tttttaacag cttcgcaaag
    16141 tttaccccac catgctggat tgactccacg tgacacaatc cattctgtta caccagttgt
    16201 gttaacaaaa gtaatttttt gcaatagtgg atgagattgt tctaaatctt caaatacacg
    16261 ttcaaataca gtagctggca ctaattcttc gacccctgca aaaccttcgt ttttcactac
    16321 ttcgttatag aattttgttt cttgtgtagt taatacacgc tgaccacggt tcattaatac
    16381 taattgatct tgattttttg ctgttgcttc ttctaaaatt ttatcctgaa tttccttaga
    16441 taagcttacc atagctgcgc taaaagattc ttcgttacca tctttaaaag ctttcatcaa
    16501 ttggtcgctt gcagctgtta caccttttaa atttttaact gtcattattt tacatctcct
    16561 tgtccaaatg ttttatttaa tgctgctgta aatgcagcaa ttttttctgc tcttttttct
    16621 ttaacgtcat tcaaaatttc ttcaacgctt tgttcttttt tagcttcagt acctgagcta
    16681 ttttctgcat cgataatttc atcgaccaat ccataactca aagctgtttc tgcatccata
    16741 aacgattctt tttcaagaag ttcttgcaat gcttcatctg tgccattgaa tcgtgtttta
    16801 tatgaagcct ttaccgattt atcaattgat tccagttggt cagcaatcgt acggaagtca
    16861 tcgacatttc cttctccgta tgtggaagcg cggtgaatca tcaattgtgc attgttgtag
    16921 atttttatag tatcgccagc cattgcgata attgaagcag cactagcggc taagccgtta
    16981 atcacaacgt taacttttgc tttatttgac ttaagtaagt tcccaatagc aatcccttga
    17041 aatacgtctc caccgtttga attaattact acttcaattt cttcttgatc acctagacta
    17101 tccaaaatat ttttgattcc cttgtcagta ttcccttcaa agaaccaact agaaccaata
    17161 aatccctgaa taaaaatttg cggtactgcg ccttcattct ttactgctag aaatgttttc
    17221 attgtcgtca ttcgcctcac ctcctttcga tacttgttga ttgtttttag ttataaatat
    17281 ttcatctgcc atcgccttat cagagcgatc atttccaacg cgttctcttc cttcgttgat
    17341 tgtaaatact ccatttctaa tgcctacatc aatagcgtca accaaatctt tgaagctagt
    17401 aatcttgatc atagttgtat ccacacgtac aaaattccct gacaagtatt cttctacttc
    17461 atagagacta gcgttaaacg catcctgaat aagttcagca atcggtatga tttcgaacat
    17521 taaaaaagcg tccacttgat ccgataaccc actcatgtct ccctttagta ggtttttcgg
    17581 aacgtgaaac gctgctgctg tcatctcaaa gatgtcgtct attaagtttt ttatatctct
    17641 tgaattgctt tggaagtttc cgctgaaatc ttctaatgtg tactcatttt gtaattgaaa
    17701 taccgcacct gcattatcag cttccataaa agccttaaat tgtgatgtca tcattttatt
    17761 gatttgatct tgtgttgtat tgtcttgcgg tcggaataaa ttccctttca gtacgtatct
    17821 acgagcgtta gagcgcttgt aaacattcat ggcactagaa atgagtttcc catacgcttg
    17881 ataatacgca tcgactagtt gcctaatttg ttgatctgcg tattttatat agataacatc
    17941 actttctaga aattctctat caaggactat gttgttaatt tgcacttgag aaaacacatc
    18001 atctttcaat gcatattctg tgacatccca actatccgca ataaatattt cgctagaatt
    18061 attagacgga gaaacgatca atacttcatt gtagaatatt aatctcctga tcagtttttt
    18121 tctaaattct gttgcattat ttttcttatt aggagctaca ttcagcctat agtaaagatc
    18181 attcttttta ttttttccat cttcatatga cttgaattcc gctttactca tcgcatttgc
    18241 aatcaaatca atacaagttt caatcgcaaa ttttcgatac acaaaatcaa cttgcaattt
    18301 acaaaagtat tcttctaaag gaaccgttgc tttttttgtg aagtatccta ccgccttttg
    18361 aaaaatcccc actttctcac ctcctttcaa gttagaatac tagaggagta aatccagttc
    18421 ctgtattttc tactgagcta tttgtgactg ttacaggagc agaatcataa atatcatcta
    18481 aaaaattcaa accatgaagg aatgaaaaaa agccatccgt ttttctagtt tcaggttcta
    18541 ttttttcata gcgtatattt ccattagaaa tatgctcttc atatacattc atgcaatacc
    18601 aacgcataat cgcatcgtca ccaaaaaata aacgttgatt aataaaaagg tcatcaacca
    18661 gatcttttaa cataccatgt gtaacagatc cgcttcgaac aatttccaca gtaaaacctg
    18721 cttcttctaa agcgggcttc aatatttttg cacggtacat atccatagcg atttttttaa
    18781 tataatattt attactcatt tcaagaaacc aacctacaat ataatcagct tctatatttt
    18841 ttccatgaac gatctgtgat tttccttgat ctatagaaat atctataacc tctcgtttga
    18901 tgttttgtaa tcgaagggct gattcgtgga taaaagtatg ttgtgtaaaa taaacatctt
    18961 tatcgtattt tcctagcaac ccaacgctgg caaaatctcg tctatcagca aaatcgactg
    19021 ttcctatcac ttcatccatt ttttcaggaa attctttttc tttcgtatgc agaacatcat
    19081 catatgaagc aacagcaaat cgtgtatctt ccataggtct gttcattcgt ttggtcatga
    19141 acgtaagtct taaaccagca ttacgttgca tttgagagta ttcttgaaac attttccgtt
    19201 ttaaatctgc attgtaatta atagttggac aagctttttc ccacatgtcg ggatcatcaa
    19261 cttcattatc gttatccaag cgacaaataa atggaaacaa actagaaaat tctgctccat
    19321 ccttgtcaat tccaagttct ccagaaagaa tcatttttga ttcttctata atgtcatcaa
    19381 gcggaccacc acgaacatga ccattagttg tatcataaaa ttctctataa tctcgaattt
    19441 taccaccacc agaagtagcc acatttatca ttgaataatc ttcattttcg tgaatttcat
    19501 caaagcggtt tgcacctggt cgcttcccat cttttgttct agcatttgcc gtgttataac
    19561 gaagtttgct gtttgtagcg atattttgaa taacttcctt cgtagcttta aatacttttt
    19621 tatctaaatc aggatgatct ttaattactt taaatacatc atcaaaacta gtctttgctt
    19681 ggctttcatt attggcatag atatcaatat cataattttt aataccgtgt ttagcggtta
    19741 gtagaaagaa gttgttccaa gaagcaaaac cagttttacc attaccacgt cccattaatg
    19801 aaagatatct attgaacact agtgttttat cttttttcca tcgaacacca taaataaaac
    19861 attgtagaaa tttttcccac ggaattaatt cgaatggaaa gtattgtgct ggtatattga
    19921 ttgaatcctc taccatctgc ttatcgaagt aaatatcttc tctagtaaag actctttctt
    19981 ctagataatt ttttagcaat aattgctctt tgcatacctt gatagtgcct tcttctatag
    20041 ctttgaacca attttcaata tgcttataac tcaggaattg attcatttgc ttcacctacc
    20101 aattcaggag taatggcaag tttatccaac atcaatccca tttgtttgtt gacagaaaca
    20161 agcaacgcta ctgattcatt ctttttacca ttctccagtc taatgccgtt ctcggatata
    20221 tcttcttcca gtgatatcgc cgtttcccat aaactgatat aacgatcaac attatctaag
    20281 aatggctcaa tatttgtttt ctgactttcc aattggctta ttaaagagcg gcgtaatttt
    20341 tctctgtagc gattttgaga caattcgttt ttaaacattt tagccctcct ttcatgataa
    20401 agttcgaaaa aatctctttt cctgacagcc ccctccgttt catcaccccc aaaaaatttg
    20461 cgatttattt taaggggggg ttatctcacc atcggaatga aagcttcagc gaagtcaatg
    20521 taataattaa tctcttcaat actatatcca aaaacatttt ttattctttc gacgttatta
    20581 tctttattca acgcttctct tacttgattc actttgtatt tactacaaca gttatctgat
    20641 aacagatccc taatacctac atagcgaacg tatatcaaac gtttaattaa tccctgagta
    20701 taagatgaat actcttcaat cttttctgta tcatactctc tgccattatc attgatgatc
    20761 atgcacttac cacctttcac ttgcatcgaa gttagcaaag ctttctatct tcttctcttg
    20821 tttatctaat gctgtaagat atctgccatg aacttcatta tgatgttcaa cacataaaca
    20881 aataagatta tctaaatcta aagctaagtc aggtctatcc ttgacttcct ttatatgatg
    20941 aacgttctct actctatgat acttacctag tcttctacac tcttggcatt catagtgatc
    21001 tcgtttcatc gctttctctc taagcctgcg ccatttagga gactgataga acttaaccaa
    21061 acgatcttct cttgtcaact gtaatagcca tctatagaat tcctcggtca tgttccgtct
    21121 cctttcgcaa tcttatttaa tgcttagcta ttcttttgcc atacaatgga ataacttcat
    21181 tgttttcctt tcgtttatat gtatcgctct ttattggtct tctatactca tatacttttt
    21241 cgccattacc tttatgaaca gtgattactt catacttctg ttctaagtat tgtggtctat
    21301 acattgttgt ttcctccttt gtgcaaaaat aaaaagacca ctcaaagagt gatctaatat
    21361 gtaatagtaa cttgcacgat gcacaaaacg cgtacgaaat tgcgcacccc tatattttta
    21421 aaccgccgat gcctcggttg cttaaagtcg ctggagtggg attgcaccac tcacgagaac
    21481 ttaccaggct ctcacgaggc tactcgccat ttaccgctgc gtcttctact tccgccacag
    21541 tgaccgaagc ttggtggtgt acacagatag cacacttact acattgccgt acgtagcacc
    21601 gtatagcttc ttaacgatct tttttcggta gtcgtaaccg tcataggcat ttaatgtcgc
    21661 tggcaaggaa tcgaaccttg catggttgcc gaagcattga ccta
//
LOCUS       DAZUMO010000044        21432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000044 DAZUMO010000000
VERSION     DAZUMO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.044"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..947
                     /locus_tag="ACG8ER_001751"
     CDS             78..947
                     /locus_tag="ACG8ER_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5050328.1"
                     /translation="MKKKEFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            967..1314
                     /locus_tag="ACG8ER_001752"
     CDS             967..1314
                     /locus_tag="ACG8ER_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050329.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1327..2589
                     /locus_tag="ACG8ER_001753"
     CDS             1327..2589
                     /locus_tag="ACG8ER_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050330.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2669..4045
                     /locus_tag="ACG8ER_001754"
     CDS             2669..4045
                     /locus_tag="ACG8ER_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5050331.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            4042..4671
                     /locus_tag="ACG8ER_001755"
     CDS             4042..4671
                     /locus_tag="ACG8ER_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050332.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4690..5169
                     /locus_tag="ACG8ER_001756"
     CDS             4690..5169
                     /locus_tag="ACG8ER_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050333.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5728..6327
                     /locus_tag="ACG8ER_001757"
     CDS             5728..6327
                     /locus_tag="ACG8ER_001757"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5050334.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6343..6819
                     /locus_tag="ACG8ER_001758"
     CDS             6343..6819
                     /locus_tag="ACG8ER_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050335.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            6836..7738
                     /locus_tag="ACG8ER_001759"
     CDS             6836..7738
                     /locus_tag="ACG8ER_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050336.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7811..8005
                     /locus_tag="ACG8ER_001760"
     CDS             7811..8005
                     /locus_tag="ACG8ER_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050337.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            8110..8538
                     /locus_tag="ACG8ER_001761"
     CDS             8110..8538
                     /locus_tag="ACG8ER_001761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050338.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            8773..9360
                     /locus_tag="ACG8ER_001762"
     CDS             8773..9360
                     /locus_tag="ACG8ER_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050339.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            9360..10259
                     /locus_tag="ACG8ER_001763"
     CDS             9360..10259
                     /locus_tag="ACG8ER_001763"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS5050340.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            10259..11173
                     /locus_tag="ACG8ER_001764"
     CDS             10259..11173
                     /locus_tag="ACG8ER_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050341.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            11225..11440
                     /locus_tag="ACG8ER_001765"
     CDS             11225..11440
                     /locus_tag="ACG8ER_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050342.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            11630..11884
                     /locus_tag="ACG8ER_001766"
     CDS             11630..11884
                     /locus_tag="ACG8ER_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050343.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            12063..13112
                     /locus_tag="ACG8ER_001767"
     CDS             12063..13112
                     /locus_tag="ACG8ER_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5050344.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKLLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVSGTYQGIMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            13287..13517
                     /locus_tag="ACG8ER_001768"
     CDS             13287..13517
                     /locus_tag="ACG8ER_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050345.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            13553..13777
                     /locus_tag="ACG8ER_001769"
     CDS             13553..13777
                     /locus_tag="ACG8ER_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050346.1"
                     /translation="MIDISLIEKLLFSNKYTAKELATRWQISESAIKAYRQNKGSVGW
                     RDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            13994..14869
                     /locus_tag="ACG8ER_001770"
     CDS             13994..14869
                     /locus_tag="ACG8ER_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050347.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKEE
                     ITKMKPVIKILDNKE"
     gene            14879..15673
                     /locus_tag="ACG8ER_001771"
     CDS             14879..15673
                     /locus_tag="ACG8ER_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050348.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNIL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            15861..16820
                     /locus_tag="ACG8ER_001772"
     CDS             15861..16820
                     /locus_tag="ACG8ER_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050349.1"
                     /translation="MGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFN
                     EAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMLFLVNFEGTAK
                     EGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKK
                     GLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETNTFDKYSMYNRAYS
                     SMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTI
                     SKEEVEKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            16853..17173
                     /locus_tag="ACG8ER_001773"
     CDS             16853..17173
                     /locus_tag="ACG8ER_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050350.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            17428..18321
                     /locus_tag="ACG8ER_001774"
     CDS             17428..18321
                     /locus_tag="ACG8ER_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050351.1"
                     /translation="MRTHYIYLEDKIIDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            18341..19975
                     /locus_tag="ACG8ER_001775"
     CDS             18341..19975
                     /locus_tag="ACG8ER_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050352.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNNGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMSLTIKRIYDLLYVYKH"
     gene            20004..21173
                     /locus_tag="ACG8ER_001776"
     CDS             20004..21173
                     /locus_tag="ACG8ER_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050353.1"
                     /translation="MTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRTPRIY
                     KNRSKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLGSKPRKARDVASVLQHIMDG
                     DYKLNQSNLIFDGRKLFFNLTISFEKRYNELEFKEGNELIVNFADLCSGECLVNNKSV
                     MSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVNKGKRYGIEVRS"
BASE COUNT         8252 a         2776 c         4368 g         6036 t
ORIGIN      
        1 atacatttga caaaatttac aatatagttt attatatatt tatcttatta tgataacaca
       61 ataaaaggaa tgatggtatg aaaaagaagg agtttagtcg atcgttgtta ggaattttag
      121 ttttagttgc ggttggtctt agtggcaagg gttactttca gaataacagc actcaggaac
      181 atgattttca gatcaaaagt agtgttcagg taggtaataa agcagacagt gaatcaaaag
      241 aatttttagg gatacgttat gatccaaaca aatatccaaa taatatcgta aacattaatg
      301 atgggaaata tacactgaca aaagaagacc atgaagcatt atcagaaaaa ggcagtcaaa
      361 ccttttgggt aaattatgca gatttagatc aacttggaag agcaggagat gttgaagcgt
      421 tgatcaccta tgatgctgta aattctcact catctaaaaa tgtggaacgt ccatctttca
      481 aaagttctgt ccatattgca ggagaataca aggatggagt atttgatcaa gagagagata
      541 cttgggtcgg caaagattca aacaatgaaa tcactcagct agaaggatat aaaggttatc
      601 tctataacaa aagtcattca ttagcatggt cgctaggagg agatatggag gtacacaatt
      661 taacacttgg aacgagagcg caaaatgttg ggaaaaatga tcagaatgga gggatggctt
      721 actcagaaac gattgtacgt gatgcaatct ataaaaataa gaatgaaaag gtattgtata
      781 aagttgatcc aatttataaa gggaatgaat tggttcctcg tggttctcat gtcgaagctt
      841 tctcagttaa cgataatgga aaaacggtaa accttaatgt ttatgtattt aacaatcaaa
      901 aaggaatcaa catcaactat caaaatggta cttgggtaag agattagaaa ttgagaaggt
      961 gaagaattgg aaacaataag catcaatgga ttagataaat cagttgcaaa tcgaataaaa
     1021 aaaggaaatg gctactatct tctgaagaag ggtaaatatt cagttgctag agagttagaa
     1081 acaatgagta tcgtgtatgt agctaagaat cattttgacg tagaacaggc tatttttgat
     1141 ccagattctg aaatagggaa atgtgttgaa ttatcagaga aaactgttag tacaaaaggg
     1201 gcgatgatca ttgagagaga aatgttagac gaaatcagag taatcattcc taaagatacg
     1261 cagatgtgtg aggacagcat aagtgacgat gaaccaaaat cagaaagaaa ataaggagat
     1321 attcagatga gcgaaaaatc cattgaatta aaggaatata agcgaagcga tggagaaaaa
     1381 tattatcctg tagccagttt ttctaaatca gtatatctgg ttggacgtgc attggtagtt
     1441 ggtgcaattg gcatatatat tttgcattgg gtaacaaagg ttgtacaact gcttgtagat
     1501 accttagtaa gtattcttgt ctcgttaaat gatgttaacg gaacagatga tttaggatta
     1561 aaagaaatgt ttaaagagtt actcaatgcg ttttttccca attatttaag ttattcatat
     1621 aatgtacaaa gcttttttgc aaagctacac ttggtagaca tctctatttt agtattagaa
     1681 gcaggaacgg tattctttgt tggatatttg gttactttga tgttcaggca tcactatgga
     1741 gaacaaagac cattttttga tgatatggaa agtagaaggt tgaaaagaga agctctagaa
     1801 gctatggatg tgaagtatga tccaaacaag attataggag atgaaacgaa caacaaaaaa
     1861 agaaaacgaa acaaaacgca attagaatcc gatgtgctat caacgcttaa aaatatcaaa
     1921 atttctgttc atacaagacg tgatctaagt gtcaatgaag atattcgaga atatatcgta
     1981 aaaatcccgc aaccaaaagg gaagaaagtt agagaaacag ttttacgaca aattcaagat
     2041 ttagggtcga tcctgacaag agcaacaagc ggacgagcca aatttggtga aaaagaagat
     2101 gaagtttcag gcgattattt cattttccga gcatcagtaa atattgaaag agaacgtaag
     2161 gaaagagtaa gtaccaaaaa caaagaaacg gaaagagcaa catcttcaat cgcagtttca
     2221 aattcacaat tttcttttcc aatcaatctc tttgccgata attccaaaaa tatccaaaac
     2281 caaaccgaaa aagctagaaa atatgccgaa actcaaaagc aatctttaga actttatcta
     2341 tcttctgctg acgtacctgc aagtctaaca gatatgcacg ttgggaatac atctattgaa
     2401 tatttgttta ctttatctcg taccagaaaa attcaactaa gctcaatgaa agaagaaatt
     2461 gaaacttact tgcatatatc aggtgtaatc gtgcggatca acgcagggcg tattcaaatt
     2521 actttaccat taggagaaaa tgatcgtatt ccaattgatg taccatcaat gatcaatagc
     2581 gtgttttaaa agacatcaca aatagaaaca gaaaatacaa actaccagtt cacaaacaga
     2641 aaagtaccga gaaaagtgag gaatagaaat ggctatgaaa ttattagaga aagtcaaaaa
     2701 gagagccgaa aaagctgaat ctattttgct agataaagaa cctaatataa agcttaatgg
     2761 aaaattcagt gtcaatatgt tgaacacaaa ggctaaagac ccgacagatg ctatttttgg
     2821 aattaagcct gatggcacaa ttgctcattt ttgcatcagc gaaatgccac acatgttggt
     2881 tgcaggaacg acaggatcag ggaaatcagt attactgaat gaaattcttg ttacagcaat
     2941 gtgtcactca acgcctgatg aattaaaaat aggaattgta gacccaaaaa gggttgaatt
     3001 tggaagatac aaaaaattgc cgttcatgtt ggctgatcca attacagata tggatgaagc
     3061 ttatgatttt tttgaatatc ttgtgatctt gatgcatgaa cgttacaaac tgatggaaaa
     3121 agcagggtta caaaacatca cgctatataa cgaatatgcc gaaaaaaatg gattagatag
     3181 atttccctat gtgatcttat tagttgatga atattcacag ttggttggaa cacataaaga
     3241 agtagaaggt ttgattgttc aattaggaca aatggcaagg gcagcaggta ttcatgttat
     3301 cttagctaca caatctcctc gatcaacagt tgtcactggt attatcaaag ccaattttcc
     3361 gtcacgtgtt tgccttatgg tagcaagtga tttagaaagt aggatcgtat tagatgaagg
     3421 cggtggagaa agcctaagtc caaagggaga tatgattatt aagctcgtta atggaagtat
     3481 ggttagagcg cagggagctt acatcagtaa caaagaaatt gaaacaattt tcaatcacct
     3541 tagaaatact atgccagaac cagaatatgt tgattaccga gcaatcgtag ctgaatctcg
     3601 tggtgagggt ggagaagacg aggattttgg tggaattggt aaagctcctg taagaaaatc
     3661 tgctaaaaga aatttgaaca cacctttagc aaattcatca tctaaagagc catcaattga
     3721 aaagaagagt acgtttgctc taaaacttga aaaaaacaaa gaagaaaaag aaaaacaagg
     3781 agctaaaaca ctagaaaaac ttcaagccga aaaattagaa aaagttttaa aagcattaga
     3841 taaagctgat tctgaaccgc aaacctcaaa caacacgaat aatgaacagc cgatttcatc
     3901 taaaactaaa ccgaaaacta ctgatccgaa agtttcagta aacgtgacca tcacaaccga
     3961 taagtctgag aagaaacaag taaacggaac gacttcacct atcatgcaaa aaatgttgaa
     4021 agccaaaaaa caggtgcata aatgatcatt gtcagttctt ttttgaggtg gcttacgcca
     4081 tataaatata cagatgaagt agttgtgaaa cgaacgaaga aaagagataa aaaagcggta
     4141 ctaactccta aacagatgct tgatcatggc attttagggg tgaaggtttt agaagtgaat
     4201 aaaccgtttg aatcaaatag tgcaagagaa aagttgcttg gagctgaagc cgatgtcgca
     4261 tatttttacc atgacaaaag caagcatcaa atagctatta aattcaatcc aaaatcggca
     4321 tggcggtatc aagcaagaga tgacttgaaa cgtaggaaac ttagaccaat gctctatcca
     4381 gaaggaaaag aagcatttga tagaacccac atcgttccca ttggttttca tggtacagaa
     4441 tcagataacc gcctattagt aggttggaac agcaatcaaa atagagttca gatcaataaa
     4501 tttgagaaga aagtaaggaa attcaacgat cgaaaaacga tcctatggtt tgcagatatt
     4561 tgtaaacagc cggatggaac agcaaagtgg gaagcggtga tatttgacga aaatggtaaa
     4621 aaagtaatgt ctgaaacatg gcatgataaa gctaaatttg tttggcagta gcagtagagg
     4681 tgaaaaagat tgctagaact ttttgaagca aatagaacag gctttaatca aaaattagaa
     4741 gtgggagaaa ctatagaata taaagataaa aactatatta ttatcggaat ctataacacg
     4801 agaattaaca gaataggtga tccacgtata accagtgact tggtttgtca atctgttaac
     4861 tattcacctg atctgacaag caggtataag aaatacgtgt tattagaata tagacacaag
     4921 attactgatg aaattggtgt aaacaacaca cgaaacatat ttaagatcgg aacagtgatt
     4981 ccttatagta acaacgaaga aaaaatattt tatcagattt acggaataga aaagtttgaa
     5041 tatgaacatg tcgatctgct agtgacgtac caaatgcgtt taatagaacc ttggtcacag
     5101 gcagaaattg ataaagcagt aaaattgaac cgattatcaa aatttaacgt attaggaaat
     5161 gccttttaaa aatcgcaata cacaaacaga aaaataccaa aaaattagca atgaacgtag
     5221 gtaagaacat gacaataaat aataataacc ctaaaaaata taatgctctt gtccaatgct
     5281 atatcgaaaa caaaattaca atgcaagaag tcgctgatga attaggcaaa tcaaagagcc
     5341 tagtatcaca cgattttaag cacaatgttt ttccgaaccc gattgagctg aggaaaaaat
     5401 ttcaaaagag agtttatcaa gaaataggta agtggattga tgaagggaat gcaattgacc
     5461 ctttaattga aaaatatcct tttttaaaaa gcaaaaataa aattcaaagg ttaattcgtc
     5521 aaggcgatat tcaagctaaa gaacctgttt tgacaaccaa tgatttggct aaaataaatc
     5581 ttcataaaca aattattgaa gactttgaaa aaaatctttc aacaactgaa attttaaaaa
     5641 aatacaatat tccgagagga aagctaacat cgactctttt tagagaatat ggagaatcgg
     5701 cagttactaa agcaaaggaa gtagcgaatg agagaaaaaa acaaaaatag tagtcgatac
     5761 ctagtagcga ctgtagtttt tgtttgtgta ttgattttcc tgtttgattt tttgcagatt
     5821 ggaaaaagca taattggaaa tacattgagt gcaaaggaaa tggttgcttt aggtggtttg
     5881 tatgaaggac acttgttccc gaatatattc atttctatcg tttctcatat taacttgtac
     5941 catttaattt ctaatttatt gttcttagta gttcttgaag aagcactgaa tgatcagtat
     6001 gagtggttaa cattattgat cgttttaatc atatcaggat ttctaggaag cttagttcct
     6061 tttctgctac acagcaacat cgtaagtatg gggcttagtg gaggtatttt tggatggatt
     6121 gctttatttg cgattgcata catccgtttt gatagcaaga aacatttatt actaagtgtt
     6181 tttgtattat tgataatcgg cattttactg gtatctactt ttgaatcagg cgagacaaac
     6241 gtcttgtcac atgtaatcgg atttgtctgt gggatgatct tgggatttat tctaaagaac
     6301 gtttcattga tcaaacaaaa gaattaaaag ggagtgctaa aaatgccaaa aaaaaaagaa
     6361 aagaaaattc cagtgttttt gtctgtctca gaaatgttat cacttcaaat actgttaaga
     6421 aaaaagattg aaacaataga cgaagaagtt agaaataaaa gggctatgta cgaacaacgt
     6481 gaagcgctaa gagaacgaat ccactatttt gatgatatac acactaaaat ctatgaagca
     6541 cgtacaaatc acgaagacga aagcaaatta ggattagctc aacaacgaat atttgaatgg
     6601 ctgaatacga atactgagca agaaccgtta agagcaatta gtaatctttt tgaatttgga
     6661 acgaagcatt ggtttaacgc tgtagaaaag gatgctgact tacaagaagc gttcgataca
     6721 ctaagcgagg atagcaaatc catgcgagac ttattgatca cttatctaaa tgagcatgaa
     6781 tttgaacaga cgaaagttaa aaagaaaaat aaaaaataga aagttgtgat gtatcatgct
     6841 gtttacctta ttaggttatt tatgtttagt tcttgctttt tttggagcaa ttgctatatt
     6901 tgtcggtcag tttcaagcaa ataatccgat aatgcgtaac ataggcattg gaattgttat
     6961 catatccttg attgtatttg gtgccacaaa gatttttggg agtaatgatc agcagtttgg
     7021 tatttctgga gttgataata gttatgctcc agattcattg ggaagagtag tgatagatgg
     7081 aacgtttgag aatgtaacga gctacaaaaa tgtttcattg acagtgaaag aagatgacaa
     7141 gaaagcatca gatgacgttc tcgctacgat caagccgtct caatcacagt ttgaagtgtt
     7201 ttatacagtc aaagagaata accctgtacg caagctaaca ttagttttta acgttgacgg
     7261 agaagaatac tataaagatg taaaaatcaa taaagcatca acgaaaaaga ttgtcgaatc
     7321 agcgaaaaaa gaagtagcta cagaagaaag tacaactgaa tcaagttcta ctgtcgaagt
     7381 tgaactacca gataaaattg attttgatcc tgaaaaagtc aaatcgtttg aatctgtttc
     7441 tggttctatg gcagatcggt ttaacactga agttttcaaa gctatcaaaa ctgttaaatt
     7501 ggatactgaa aagcaaagga ttaatgttac cttattcaat gcagttgaat caatggatca
     7561 ggataaaaaa gttacggttg caagagaact agggaaaaga gtaattgggt tgtatcgagg
     7621 tacggcaaaa ccagattcag acggttcaga tttgccagtt cacgtagttt atgaagacgg
     7681 taaagttttt ggagactcta cagcaaatga agcactaacg attaaattag ctaaatagcc
     7741 ggaaaattat aataaacaag tggtgtcaca ccaccatcgc ataggtgtga caccttggag
     7801 atgattactg atggtagtaa ataaagaaaa agtaagaaag tatgttactt taaaaatagc
     7861 ttctgttgaa tatttagaaa agcgagctaa agaagaagaa aaaacaagag gtaagaaggt
     7921 aacagtttca gagttgttag aagaattaat tgagagtgac caaacaataa gacgtgcatt
     7981 taccaatagg tctgttaaaa gttagggtga tactatcttt aatttcaacc aaatttctaa
     8041 tatatgatga atagtttcct tgtacaaaat atctaattaa gagataaaag ttaggaggat
     8101 tcattcaaaa tgattatcca aaagtttgac tggaaaatga aattaggaat tagtcttgta
     8161 atattgggat ttattatttt tttggggata aaagcacaaa aaaatcaatc tacttttgaa
     8221 actatgtctt ctcaaattat tgaggaatgt tccaaaattt attattgtga gaacaaaact
     8281 gaagaatcta atggctttta ttatatcgat caaccacatg atgttgttat aaaaatggag
     8341 tcacatgaag taaacgaatg gattcaaaga agtactaatg tatcaaaaaa gttagaaaga
     8401 tattctcaaa cgtttttaag taaagtcatt ttttcaaatt gcaaaaatga agaaacgtct
     8461 caaagagtag ctagttcttt agaaaattta aaattttctg tttcaaaaaa agagaaattt
     8521 gtagatttac gaaaataaac tatcgaaagt agtatgatta tgatcttaaa atatcatctt
     8581 aagagaagaa ttacgattgt ttataggatt tacaaaatga ataagtttga agagtgattt
     8641 ggtcgcagac tagatcactc tttttctgta aaaaaacttt acacttattt ttgagtgtgt
     8701 taagattttt tatatagagt gacggtttct tgtcagagtg aatgataacc atcaatattg
     8761 gaggaataat aagtggaaaa caataatgat attagatatt ttagcattgg ggaaagagtt
     8821 acaacagtaa gtgaattatc aaaggattat agagataaag aaggaacgat tatttctctt
     8881 aaaaagaatc gaagaggtgt ttggcaatat gttgttgatt taggggatgg cgttacagtt
     8941 gagtgtaaag atagtcaact gatcagaaat gatggcagtc attccattga aaaagcaatt
     9001 cttgtattcc caaaagtatc aagcaagatt atgaatgact ggttgcttgg aaaatttggt
     9061 gagtacaata aaaaagaaat cgcagaagtg atcggcaaat cttatccaac agtattaggg
     9121 tatctaaaaa agataacgaa caaagaagcg aaagttgagg atgttttaac agtaagagaa
     9181 cttcagtcat taatcgaata tcggaataaa caaataacaa atcctgagta ccttaaaaaa
     9241 gaagatggtc tattgaaaca attggaacag gtaaataaaa tccggaaaat gcaaaaggta
     9301 gctcctttaa gcctagataa ctttaaaaaa atctatttga aagagatcga aaaagactaa
     9361 tgtggagtgt cactttaccg tttcaacctg tatctgcacc aagaccaagt gcaagaagga
     9421 atgttgctga tgcaactaat gacgtagcag agaaaaggat ttcaacctat tatgataaaa
     9481 agtatctcga ttatttgaaa aaaataaaaa gctttatcga agatcaaggt ttgttagatg
     9541 atgaatttta ctcgatcgta aatgatccga tgggtgcaat tatggaagtg gacttttact
     9601 atcagatacc aaaaagctat aaaaaagtct ataacattat gaagacgaca gcgccagatt
     9661 tagataattt agtaaaaagt gctatggatg gtattttcaa catctcagct atacgagata
     9721 gccatatcac agggttaatt gcttttaaat acaatgtggc aaacgaagga aaaacagttg
     9781 tgaggatcaa gcgatattca gaatttgagt gggatacttc tgaaggattg aacggagata
     9841 tttgggaagc cacatttgat ttcaaaccag tagcaacgcc aagaccaaag tctaaattta
     9901 gaggaaatgg gattatcact tactatccaa aagagtataa cgactatttg gaaaatatca
     9961 agaatacgct taaagagaaa gatttggtaa atgatcaact gaaaaaggta atgaatgctc
    10021 ctatgggggt tatagctcag atagattatt tttaccaagt gagaaaagat aagaaaaagc
    10081 ttgattcact gatgcgtact tcacagccgg atattgataa cttagtcaaa ggaacattgg
    10141 acggaatttt taacaaggaa attggcatca aagatagcag ggtggtaggt ttaatagctt
    10201 ttaaatacaa tacttttgaa aagtcgaaaa caaacgtgcg actacaggag atgggctgat
    10261 gaataataac gaaaaaattc ttcatgtact tgatagcttt gaaacaattc aagaggaact
    10321 aaagaaatat cgagatgttt tggaacaaag atatgatttt gtcaatcagc aaaagagtaa
    10381 tcacatggat ttcattctca atatgaatga cttgaagaag aaattagttg aacgaaaaga
    10441 acaagaaaag ttgatcaaag cttactttga actcggtgaa aaagaagtta aaaaggcaat
    10501 ggaactgaat gaagatcgca gggttttaga tcagctgtta gaacaattat tggtgatgtt
    10561 tcaaaaagga cgaatagatg aggatttaat tgaagaaggg ttgagaaagt atcctgcaaa
    10621 ctcaggtatc ggaatagtgc tgaaagcgat tgatgaagaa gaaattgaag atttcatacc
    10681 ggcagaggat tttgaatcgg caatggaata tataaaatat tacagccaag ggatcactgc
    10741 ttttagagag tttgatcctg aagatgttat tcatgacttg aacaacctaa aagaatggtg
    10801 tgaaggttat gaagttgatg attccggttt ggactacttg atcagtatta tggaaatcga
    10861 agaagagatg ccggataagc ctgatccaac agatatttta gaattaatcc atgatgctag
    10921 aaatccaatt gcatacattt ctagaggata tactgtattg gaatactata agccttatat
    10981 atcagcgatg aaccatttaa gacgtgtatt aagggaaaaa cgtgaatacc gctcagtgtt
    11041 aaatgcatcg aatagattag aaaaagctgt ttcagagcta gatgcttatt atcgtgaaca
    11101 ttacctgcaa gcaggtggta tgccaagaaa tacgaaagca aatatcagta ggtatataaa
    11161 agaagctgag taggcggagt tagcccataa aatggcaaaa ggtaacctga aaggggcgtt
    11221 ttgaatgaaa agggatgttt ttgaattgta cgtattagaa cgtctagaag acggcatgat
    11281 ggcacaagag ataattgatg aaatcgatga tatgatcagc agatttggtc aattggaatt
    11341 gaatgatgga aacaaaaatt cggtagtgct acaacaattt tggttaaata atttttcgta
    11401 taaggatttt atcaaagaaa aggcaaatag attgtactga ctaaattcac gaggatcagt
    11461 gatgagagct tattgctaag agcagttagg ctcttttttg tttgtgttaa attattttat
    11521 tttacaatac acatttatac acaaaaaatg ttgacggata gtacacaaat gtggtaggat
    11581 atatttgtaa gcaagttaga ttaacaagtt aaaggaagga agagcagtta tgaaaaaaga
    11641 agaactaata gatatgtttc aaattgttga acgagcaaat aatatgggaa ttatgttttt
    11701 tgatcgtatt tctttaaaga tggatttaag cgttgcccac caagaattta atctacgatt
    11761 aaaagcttta ttgatttctg atgatgttaa tttcgctcat gatgttgtag gtatacagaa
    11821 ccatatagat agagaaaaca aacgtatggg tgatggattc ttaccaagat attcaagttt
    11881 atgatttggc gaaagattca agagtatgtg tcattattga taggtactct tgaattagaa
    11941 aggaggaatg aatttaagac aacaacgatt atgatcttaa aacaatcgtt tatgtaattg
    12001 gcaatcttac gaataatttt catgcaataa tgacaataaa aaatgaaaag gggtaaaatc
    12061 aaatgaaaaa atttttagta ggaattctat tcacaatggg gctagtgcta ttatcacctt
    12121 cagtttcagc agaaagctct atttccatgt atcgtatgta taatcccaat agcggagagc
    12181 atctatacac tagaagcgtt ggagaaagag acaatctgaa acgttatgga tggcattacg
    12241 aaggtgtcgc gtggcaagct ccaacaagtg gttccccagt ttatcgcttg tacaacagat
    12301 ataacggtga acatttttac accgttgatg ccggagaaag agataagatt tcgcacacag
    12361 gatggacgta cgaaggtgtt gcgttctatt cttacaacgg agcaaataga gttcctgtga
    12421 cacgcttgta caacaaatac gtgaattggc accattacac gttagatcaa aatgaaaaga
    12481 acgtaattag taaacaaggt tggaagattg aaggtacggg ttggtatgca atgccaaaca
    12541 gcgcaccaaa tcctacacct gcacctgctc caagtaatga gaaattgcta aatgttcctg
    12601 tcatttggca aggtaatacg atgttgtgtg aaggtgcttc cctgttagag ggattgcact
    12661 acaaaggagt agcgacaaat caaaacttaa ccagttttgt aaactatatg ccaagagcta
    12721 atgacaataa tccttatcat ggctattctg gcgaatggcg ctacaatgtg agtgggacgt
    12781 accaaggcat tatgcctgat ccagtagtta aatgggcaaa aaatgttggt ggtaatgccg
    12841 ttaatatcac tggttcagga acgaatggga tcaagaatga aattcgcaat ggtaatccgg
    12901 ttgtaacttg gattacttat aactatgctt cacctcaatt caaacaaatg ccttggggac
    12961 gtgcagtctg gaatggacat gtggttctcg tggatggctt caaaaatggt gcataccatg
    13021 ttgttgaccc tgtatttggc gcaaaatgga tcaatgcaag cacgtatgag agagcattta
    13081 atgtgactgg aatggcggtt gctgtacgtt aattgataga attaataaat aaatgaaata
    13141 agagcttaat tactgaaatg gtaaatgggc tcttattttt tagaaaaaac tttttttgta
    13201 atacgaatct ataacaaaaa acgttgaata tagtacacaa aagtggtata ttaaaaccgt
    13261 agagattatg cgaagaggtg attttgatga accaaagaag aagaaaaagg ctagaagaaa
    13321 taagcgagcg actatccgag ttaaaggatg agttagaagt aattcaagaa gaagaggaag
    13381 agtacaaaga aaatatacct gaaaatctac agggatcaga aagatatgaa aaagcagaag
    13441 aatccgttga tcaacttgaa gaagctgttg atggtttatc cgacatatta gaattgattg
    13501 aagatatagc tagctagaga tgattaatga agatttcagg agtgagcaaa aaatgattga
    13561 tatttcactg atagagaagc tacttttttc aaataagtac acagccaaag agctggctac
    13621 aagatggcaa atatccgaat cagctattaa agcctatcgt caaaataagg gaagcgtagg
    13681 atggcgtgat tggaaaggaa tcagcattgg aaaagcagaa gacataatga aacgtgaagg
    13741 atttcaagct gacaatgatt ctaaaaaagt tgaatagttt aatagtcgcc aagaaaagta
    13801 ccagggcgag gggaaaggta gattacaaag gaaaacactt ttaaataaac ttcgcaaaag
    13861 catcaggtta tgagtggcta gggctatacg aatgtgatga aaacaaaggg ctttaatgtc
    13921 aaagaaaacg caataggggg ggaataaaat ttttaaaaaa gatatttatg tttggtttaa
    13981 tgggaaaaaa tatatgacta ctaacaaaag gtggtcagag ctagtacaaa atacagttaa
    14041 aaaagtcatg ccagatggat atcatttcaa ctatatacaa tgctcacaaa caactgaaag
    14101 tatttatttt tcaatattaa atgagaatag actctattat ttcagggtaa gttcacacca
    14161 taggaaaaag agagaagata atgttacgac atttttatcg ggaaaataca atggcttcaa
    14221 agagttaagt gaagcaattg gtgagtatct aaaaagtaat agatttgtca gaatttctga
    14281 caaacatata ttgatcttgt tactttattt gaataatagc ttcaaattat tttggagaga
    14341 cgacactttc tactatattg agaacggcat ggatatttat tctggtatca acggcatatt
    14401 aaaacattat attaaggcgt tacttcgata tggggttctt tatcaagaag atttaagtaa
    14461 ccaaattaag attagtaatt ctggaaagtc attactggca aaatataaaa aattgctttc
    14521 tcctatctac aaaggaatag atatggaagc tgtaaattct gaaactttat ttaatttagt
    14581 tgttgatgaa aagtttgcga atgaattttt taaaagtaaa aaaattgaaa gactaaataa
    14641 cctgtttcct gaaaatgcaa taaaagctta tgtagataat aataaatcac gattatacaa
    14701 ctggtttatt cccaaacggt tgcgaggaaa aatcaaaatt ggcgatcaag tgaaagtaat
    14761 taatctaaat catactaaga tcgttaccgt aacggagttg tatatagctg aaaaagaaga
    14821 gattacaaaa atgaaaccag ttattaaaat actagataac aaagagtgag tggaatatat
    14881 ggtagcaaaa aagaatactc taaaagaagc ttctgaaaaa tatcaagata tgaaaagacg
    14941 aggattaatc ccaaaggaca aaaaaggtat atggagagaa tttgttaagg aagtcagtag
    15001 tgatcgaagt ggcggtgact ttgttggacg atatacagca aaaggttata gcaggcgctt
    15061 aaggatcact gttgaatatg gaacgaatga atcagctaca gagctgaagc aaaggtactt
    15121 agatacacga agagaagctt caaaaaaata tgatgatttc aagagcagag gtctaattcc
    15181 taagaagaaa aaggggattt ggcgtgagtt tttcaaagaa gaagatagca atcggaataa
    15241 gggagacttc attggacgat acacagcaaa aggctacact aagaatttag ggatcaaggt
    15301 tgaatacgga actggtgaaa gttcatggga attgaggaat cgctatttaa atatattgtt
    15361 agagaaatat aaagcagaat tattggagtt gttcgatgtt aatgatttta tcgagatggc
    15421 tgaaaagtat ggtcatgaag aaggttttat gatcaatgac attatggtta ttgataattt
    15481 tacggattta ttagcacttg cgaaaaaagc aagggattta agagaaacgc agatgtatct
    15541 attcagaaat aaaaacggaa gaaaccggag aactgaatcg agtgagattg aagattacta
    15601 cgatgccttt acacacgatc tgggagcctt tgtagacgag ataagtggtg taatgaacaa
    15661 atatagggtc tagaataaaa aagccggaga tgagcagata aatgggaaac aatagcatat
    15721 acacacctga aatgcagaat aaaattaaaa gacttgtcgg attagggtgg atgacaagag
    15781 gaaaattaga ttcttttagt acgatcaaat atcttttgat ggacatttac cgtcagaaca
    15841 agcaaacagg aatacatttt ttgggctatg ataagccgga agatgcacct ttctttgttc
    15901 caagttcagc ctatagagcc agtacctccg gtagcatgta caacttggat ttgttcgagg
    15961 aaggagcaaa gatgatcgaa tatgatttca acgaagccta ttcgaggatc atgagaaatt
    16021 atcctttgcc gtcaaacact tatttgaaag caaaatataa tacagatcaa gctattttga
    16081 gagcgataaa tagaccaaaa gtaagaccgt atgaaaattt aaaaacgtat atgttatttt
    16141 tggttaattt tgaaggaaca gccaaagaag gaacatatgc taatccaaaa tcacagtcga
    16201 tgtttgcgga ttatggtgaa aatcccaaaa cagctaatga gcctatttgg ttatcggaga
    16261 ttgagctaac acttatcctt gattattatg atattaaaga gatggaagta ctagatagcc
    16321 acacattttt gacgaaaaaa ggattactca aagaatattt tgaaagaatc gagccattaa
    16381 aaactgatcc tgatacgttg aagctttata aaaatcttag aacgaaagta tttggtgcta
    16441 tcggacaaaa atcattaaga gaaggagaaa caaatacctt cgataaatac agtatgtata
    16501 atcgagcgta ttcctccatg gtagcaggcg tatttagaga tcgtatggtt agatatgaac
    16561 agaaatacgt tcatagcgca tatgggctat taaaaattcg tactgatgga ttgtatttca
    16621 gatccgaagt accagagttt gaagagctgt ataaacttgg agtagttaag aaaaaagttc
    16681 atacaatatc caaagaagaa gtcgaaaaag ctttagaatt taaaaacaaa ggatttgaaa
    16741 ataagggctc ctttgggaac tctaaagatg aagttaaggg tgttgtgata gacgatgatt
    16801 tcgagtttga ttttgactaa atactctttg gaaaagatgg taggaggtaa atatgaagaa
    16861 gaaaattgtg gaaggaaatg taggtgttat tattctaaaa gattcaactg actatgctaa
    16921 atcaatggca ctaaaagacg aaaaagttgc acgcatggat aaagatttag ttgaatttct
    16981 gatggatgat acagaaagat taaaagggga tcagtttttg agtttaatga aagacaaagg
    17041 cttaaacaca tatggtttat ttgattataa gaaattagct atagagtggg taaaagaagg
    17101 agaaatattt tttattgaag aatgggaagg tactgagaac ttagttcttc atcagtatct
    17161 tttgaaagct taatctaagt aaaacagttc tgactagtta tgcttgtcag aatttcaaag
    17221 gatgttttcc ataaccatca tcattcaaaa ataaaggatt tgaaaataag ggttcctttg
    17281 ggaaatctaa agatgaagtt aagggtgttg tgatagacga tgatttcaag tttgattttg
    17341 actagatttt tttgcaaggt ttaagagtaa acaaggtaag aactaagatc atgaagattg
    17401 agagtaaaga agatagagga cgaatgtatg agaacacact atatttattt agaagataaa
    17461 attatagatg aaattccggc aaaaagtcga gaacaagcta gaaaattatt tattgaaaaa
    17521 tacaaagaga tcaatggaga tagtgaagtt ccaaaagtgg tcgtaacagt tagaaaaagg
    17581 caaacaaaaa agagaagtac gtttcatgtc agccctgata tatataatct tatagagcga
    17641 catagaagtg aagataatat ttctatctct caatatattg ctacagttat tgatgaattg
    17701 atttcaggaa agtatgacta tgcttttgat tttgaggatg aaaaggatta caaaagtgtt
    17761 gctgtctttc aatctgattt tgagaagcta aaaaactatg ctggcaggag aaatgttaat
    17821 tttgcaagac tactagaaga gttctactcc gaagagttgg gagtaggaaa tgaatacaag
    17881 agagaaactt atgcgaaatt gtctcataag ttatcccaag cgcttaaaga agtgacagaa
    17941 gttaaagcgg aactagatgc taaacttgaa gaagtgaaaa aatatcaaac agagaggaag
    18001 aacaacatga agaatcaagt ggaatttgac gaacagaaag aatttgaaaa agcaagaaaa
    18061 cgctataaca aaattatgaa tgaacttagc tatgaacact taacagttca tgaagaattg
    18121 agtgagggta cagaaaaata tacgatcgca gatatggtta atgaagtgga aactctttta
    18181 gcttctttct ttgaaaatgg tcacgtaaac gatgaatggc gaaaagaaga acctaaaagg
    18241 gttaacgctt atattgctcg attaaaaagg tttattgata aatataagga gaatagtgac
    18301 aaattttcta aagataaata aggagagagt gtgattacat ttgccagaaa tcgaaggaat
    18361 attttcgcca aaaataggta aaaaacagtt agatttcagc agttttccga gcgtaaatag
    18421 agattataat ctaggtgatg aggtacagat actaaatatt gatttcaaag aactagactt
    18481 cgctgttgct gtttccaaca aacccaaggc attggaacga gtgaaaaaaa tataccaaga
    18541 gaatgatcaa aatatcaaaa atatgatcaa aaagtataga cgagataatt caaaagttga
    18601 agacgtttta ttcaacggta cggcaaaaca tgatttgaat gtttatatgg cattaggagt
    18661 ggctttgtac tgtaattcaa ttaatcagac agatattttg gttaatatcg tgaggaatag
    18721 ctatcaaaaa atattaaaaa taagtagcga aacaaaccct tattatgcaa tgaaatatct
    18781 tggggataat ttattaaatg ataaaaaatt gatgcacaac actgaagagc tgttatctaa
    18841 tttggctgcg attaactttc tattcaaaac aggttctgaa gattatttta tagataacgt
    18901 actaaaaggt gcttacggaa cagcatttta tccgctttac aatatgaaac agcataaagc
    18961 cgaacaaaag gataacaaac ttgaaattaa aaagatgaaa gaactattag gtataggatt
    19021 tgtaaagccc aattcaaaag tcactattgc ttcgttagca actactaaca ataatgggaa
    19081 aatagtaaat gggtttgacg attcagatat agatatattg tcaaaatcat atttttcttt
    19141 gtatggagat tttgcagatt tgatgggtat tgatagttac ttagtcaata attttgagat
    19201 caagggtagc gagctgaatg atattgtgta tttcgtttta atctctctat atgcaattga
    19261 tcagaatacc gagtaccgac aaattgacta tttgctagtt ttttatagtt tgttatatca
    19321 gttcgtttta actcggttgt ttaaaaatgc taaggaagaa ttgatcctga attctgatta
    19381 tgaaatagca aaacaaattg caagtattag aaaagaaaac aaatctgaag taatcaaatt
    19441 aagaaaagaa ttaagtattg cgagtaaaga aaacggtcgg ttagaatctt tagtcaaatc
    19501 ggcaaaaaaa gaagaggaac ttttgaagca agagattaaa gaattaaaag gaatcagaaa
    19561 agaacttgaa cagctgaggt ttgaaaataa agtattaaag caattgcagg agcaagaaca
    19621 tagacaatca ttaaatggta aaactgaaga gccttcactg tctgaaatga tagcttatct
    19681 aaaacataaa aacattcttt tagtgggagg acatgtaaat tttcaaaata aattaactga
    19741 actattacca aatattaaaa tgtttgatgt aagcgaaaat aatggaaaga tacttaaaaa
    19801 tttgaatacg tgtgatgcga tttttctata tacagattac ttgaatcatg gtctatatta
    19861 tcgtgttatg agtgaaattt ctaataaaga taagatattt tatttagata atcatgtgaa
    19921 tatgagctta acaattaaac gtatttatga tcttttatat gtttataaac attaatgagg
    19981 tgttaaaatt gacaacaata actatgacaa aaaaaataga agtatatgtt gatggatcac
    20041 aagaagaaag ggattccttt tttgattttt tttggaaaaa tagtagagaa tatgtaaaag
    20101 cgttgaatct ggtatataaa cagctaatgt ttgaagagtt gataaaagat gcaattaaag
    20161 aaatggatca aggttatgct gagagagaag aaagtattca aaatcggatt gaaaagttag
    20221 catatactga tccaatagat caaaaaaaac ttgataaagc aaaagctgaa ttatctaaat
    20281 tgagaaatga aaaaaataaa gaagcgagag aaatattaac gcaagcgata gggttgaaga
    20341 gtcaaacgag aacacgagat gtagcaaaaa aattagactt tgactttgga gattttgttg
    20401 attccgcaaa tatgaaagct agtaaagatt ttggcaatga ttttatcgga attgtgaccg
    20461 gacaaagaac gcctagaata tataaaaata gaagtaaata tggattatta ccttttagag
    20521 gtcgagatat taaaatccat aaagatgata agttttatat ttatttacct aaaggcttta
    20581 ggtttaaagt gaatttaggt agcaagccaa gaaaagcaag agatgttgct tcagttttac
    20641 aacacattat ggatggcgat tacaaattaa atcaaagtaa tcttattttt gatggaagaa
    20701 aattattttt taatctaaca atttcttttg aaaaaagata taatgaactg gagttcaagg
    20761 aaggtaatga attgatcgta aactttgcag atttgtgtag cggtgaatgt ttagttaata
    20821 ataaatcggt aatgtctttt ggtgatagcc aatatataag aaacttcaaa agtaaaattt
    20881 tagcaatgaa atcaaaagaa caaagccgag gagtcttttc taaaggcggt catggtcgaa
    20941 aaagaaaatt agatcaagat cgatgggaag caataaagaa aagagaaagt aattttgtta
    21001 aaacttataa taatcaatgt tctgcaaaaa tcgttaaaac tgctattcgc aataaagctc
    21061 aaattattcg tatcattgaa ccagataagg cttatggcga atgggcttac tatcaattga
    21121 aagaacaact agtaaataaa ggaaaaagat atgggataga agtcagaagt tagtaaagta
    21181 aacaatcgtt gatttggcaa cgaaatagtg agggaaaatc cactcactga agggtaaact
    21241 ccgtcaagtt gtgtgaccag acggaaggac gagcgttgtt ggggttttag aaccatataa
    21301 aacagcatgt ttctcaaatt gtgtttgttt catttatgtt ataataagag ttttagagcc
    21361 atataaaaca gcatgtttct caaatgagta ctttaaatta attgatggcg ttcctgcttt
    21421 agaaccatat aa
//
LOCUS       DAZUMO010000045        21089 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000045 DAZUMO010000000
VERSION     DAZUMO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.045"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..514
                     /locus_tag="ACG8ER_001777"
     CDS             116..514
                     /locus_tag="ACG8ER_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5050354.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            751..1293
                     /locus_tag="ACG8ER_001778"
     CDS             751..1293
                     /locus_tag="ACG8ER_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5050355.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1376..1927)
                     /gene="lepB"
                     /locus_tag="ACG8ER_001779"
     CDS             complement(1376..1927)
                     /gene="lepB"
                     /locus_tag="ACG8ER_001779"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5050356.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2079..2306)
                     /locus_tag="ACG8ER_001780"
     CDS             complement(2079..2306)
                     /locus_tag="ACG8ER_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS5050357.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2303..2821)
                     /gene="msrA"
                     /locus_tag="ACG8ER_001781"
     CDS             complement(2303..2821)
                     /gene="msrA"
                     /locus_tag="ACG8ER_001781"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5050358.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2841..3554)
                     /locus_tag="ACG8ER_001782"
     CDS             complement(2841..3554)
                     /locus_tag="ACG8ER_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS5050359.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3557..4453)
                     /locus_tag="ACG8ER_001783"
     CDS             complement(3557..4453)
                     /locus_tag="ACG8ER_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5050360.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4611..5453)
                     /locus_tag="ACG8ER_001784"
     CDS             complement(4611..5453)
                     /locus_tag="ACG8ER_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5050361.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5585..6238
                     /locus_tag="ACG8ER_001785"
     CDS             5585..6238
                     /locus_tag="ACG8ER_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS5050362.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6302..6829)
                     /locus_tag="ACG8ER_001786"
     CDS             complement(6302..6829)
                     /locus_tag="ACG8ER_001786"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5050363.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6849..7796)
                     /locus_tag="ACG8ER_001787"
     CDS             complement(6849..7796)
                     /locus_tag="ACG8ER_001787"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5050364.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7808..9691)
                     /locus_tag="ACG8ER_001788"
     CDS             complement(7808..9691)
                     /locus_tag="ACG8ER_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5050365.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(9859..11931)
                     /locus_tag="ACG8ER_001789"
     CDS             complement(9859..11931)
                     /locus_tag="ACG8ER_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5050366.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            complement(11944..12219)
                     /locus_tag="ACG8ER_001790"
     CDS             complement(11944..12219)
                     /locus_tag="ACG8ER_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050367.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12581..13036)
                     /locus_tag="ACG8ER_001791"
     CDS             complement(12581..13036)
                     /locus_tag="ACG8ER_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050368.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(13190..14398)
                     /locus_tag="ACG8ER_001792"
     CDS             complement(13190..14398)
                     /locus_tag="ACG8ER_001792"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS5050369.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14510..14842)
                     /locus_tag="ACG8ER_001793"
     CDS             complement(14510..14842)
                     /locus_tag="ACG8ER_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5050370.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14990..15865
                     /locus_tag="ACG8ER_001794"
     CDS             14990..15865
                     /locus_tag="ACG8ER_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5050371.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15947..16528)
                     /locus_tag="ACG8ER_001795"
     CDS             complement(15947..16528)
                     /locus_tag="ACG8ER_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS5050372.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16534..17793)
                     /locus_tag="ACG8ER_001796"
     CDS             complement(16534..17793)
                     /locus_tag="ACG8ER_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5050373.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17795..18841)
                     /locus_tag="ACG8ER_001797"
     CDS             complement(17795..18841)
                     /locus_tag="ACG8ER_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050374.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(19076..19351)
                     /locus_tag="ACG8ER_001798"
     CDS             complement(19076..19351)
                     /locus_tag="ACG8ER_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS5050375.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19594..20904)
                     /gene="der"
                     /locus_tag="ACG8ER_001799"
     CDS             complement(19594..20904)
                     /gene="der"
                     /locus_tag="ACG8ER_001799"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS5050376.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT         5935 a         4292 c         3592 g         7270 t
ORIGIN      
        1 cacttttttg atgatgtaaa tttttatttt ggtttctata tatgaatcta aatttttgac
       61 aacgtttcct aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa
      121 tagtatggtt tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga
      181 aaagctgggt tttaaaaaaa atgaagaatt ttctactgaa gaagctggtt caatggtatg
      241 ggatgacact ttttgggtaa tgcttctctc tcatgatttt tataaaaaat ttataaaaga
      301 taaagaaata gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa
      361 cgcggatgaa gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga
      421 agtagatatg ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg
      481 aaatacatta gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta
      541 taacaaaaaa acaaaggcgg atgatacgat aagtattctc cgcctctatt tttttcattc
      601 gtacaatctc ttttttacag tttataaaag cagtcttctc accctttttt taattgttag
      661 atgccagtgt atgtaaatca ccaatgtgtc atgacacatt ggtgatttta tggtatcctt
      721 ttattattaa ctgacaaagg agagaatatt ttgaacactt tgattgcaaa agacgttttt
      781 agattgcccg aaatgaatgc aagattgttg tcgacctctg ctcttttatt agcccttgat
      841 gtcatcttat acaagatagc gattggtcct gcttattttc aaatcacatt cggattttta
      901 agtatggcgc ttatgggtta tctatatgga ccgatctggg ctggtttgct tgaaatgttg
      961 tcgaacactt taaatttcac gatatttggt tcaggaacgt ttcaagctgc ttttcttatc
     1021 acggctttcc ttggtggatt tattaatggc ttatttttat atcataaaaa gataaacctg
     1081 ccttccgtca tcgctgccca attattgatt atgatcccag tcagcctttt tatcaattct
     1141 tggctgatgt ctgttttgtt tggtgcagat ttcaaagtga tttttctagg tagagtcatc
     1201 aggaatatga tccaaatccc tattcaaata atggttgttt attcatttat caaaacttta
     1261 gaggctagaa aagtgtttaa acgagctgtt tgattttacg tactaaggta gtaaactgat
     1321 tttggattaa acaataaagc gtgcagtctc aacagattgc acgctttatt gtttctcact
     1381 ggatccattt tatttcatca agtggataat agacaaatct tgctttacct agaatatcag
     1441 ttttattgat cgtgccaaac gaacgactat cttttgaaac tcttcgatta tctccaagta
     1501 caaaataatt atcttccgga agcttggatt gtcccgttag ctcttgtagt gtaaagtccg
     1561 tcgtatagga tgcatttgca tgatctgatt tcaggttttt agttagataa ggttccttaa
     1621 ctactttccc attgatcttc aattgatcat tttggtagct gacattttct cctggaagac
     1681 cgattactcg tttgatataa atcgtgccat ctgctaattg aaaaacaaca atatcaaatc
     1741 gtctgatctc cgaaaatttt tccatcacaa ccatatcacc ttgcttcaac acgttttcca
     1801 ttgaattgcc ttccactggt accgggatga ataaaaatcc ccgcaacatg aacgcaagga
     1861 atacagaaac aagtaagtat ttgaatacaa gccagaagcg attaatatag cgttgttttt
     1921 ttgtcaatct ctatcaatcc atccctcgtt ttttcattct ttctttatta taagaaaaat
     1981 atcaaaaaaa tgcgactaga tagctgaaat aaaaataatt ctttcaatta ggctattgga
     2041 caaaaaactt ttttgatcat aaagtacaaa actatctctt atatgtcatg attattttct
     2101 aaatattttt cccatgcttc gtcaaagata tccatggacg gcaaatagtc gacattcagt
     2161 tccagataat tagaaatttc ttcgtagcta gagctgtgct taggaaattg gatgtcttta
     2221 ccggcttctg tagcgaaaac agataaagca tcatgtgtgc caccgcctcg cagtgtcatt
     2281 aaataatgaa aaaagcttcg attcaccctt cgttcctcca attttcttcg ataaaggcag
     2341 cccggcgtgc atggtttctt ttaaaatttt caggatgctt tttataatac tcttgatggt
     2401 cttcttctgc tagataaaat ggttgggctg gttcgatttt tgtaacgatt ggctgttcga
     2461 aacgtccact ttcagctaac tgtttcttac ttttttcagc aatgatccgc tgttcttctg
     2521 tcaaataaaa gatcaccggg cgataattgt caccgcgatc ctcgaattgt ccgaaagcgt
     2581 cagttggatc agtttgctgc cagtaaagtt ctactaattg ttcatatgaa atcaatgttg
     2641 gatcatagat aatctccact gcttctgtat gtccagtcgt ttggcttttt acttgttcat
     2701 aagtcggatc tggtacatgc cctccagtat atcctgacat cactgtatgg ataccaggaa
     2761 gcgtgtcgaa aggctggatc atacaccaga aacagcctcc tgcaaatatt gctcgttcca
     2821 ttatgattcc ccctctaata ctactcatcc gatgattttt tattttcttt tgtcttgctt
     2881 tcagtactgc tggtgctagt tttgttggta tttgagttgt ttgaagcatc agaggtatct
     2941 tttggcaaat atagactaaa ccgaatgtca tcatttacta agtcgatttt atctgcttta
     3001 atatacatcc cgttttgcat tcggaattga tccaacctaa gcatgatagt cagatcatct
     3061 gtattgactt cgatccaatc tggtaattta tagtttctct tgatcatatt catcacatct
     3121 ttgattggca aactcaatgt tccgatagaa agacttctag cttttagttg gacatttccg
     3181 ttttccatca catacggatc aaaatataaa tagaaatcga taggaaaacc tagtacttcg
     3241 aaagtaccat tgagtagtgc ttcattttcc aaataaaatt tatattcaat atcactatct
     3301 ttttgatact cactaaggaa aaagctgatg atttgattga ctttttcttt atttgaattg
     3361 atcgttaaga caggatctcc ttgtctttcc gtaagagctg gtatttctgg gagttctggt
     3421 tcccgattag caaaaatacg gctgcctaaa aatacggtcg aaccaagtac taccccaaga
     3481 aggataagga aggcgaattt ccagtaattt gttttctttg ttttgtttga agatacgtca
     3541 gatcttgttt tcatgtttat tgatcatcct ccttctctag ccattggttt tgtgtctcga
     3601 tcagctgatc tctcactgca tttgccatca attgataacc taaattattt ggatggaaat
     3661 gatcttcttc atagagcaca ttatttttga tgccatttag attttctgtc gtgctagttg
     3721 tttgatctgt tccttcttct gtgacaccta cttcctgatt caatccttga taaagcaaat
     3781 cattgattgg gataaagtag cagttcggca ttttttttgt caccgtttct gttcctttgt
     3841 tccaattgtt aacaattgtc tgcatgtcag tgatctcagg aaagttcaaa taaaatggat
     3901 tgtagatgcc taaaatgtaa ataggtgcgg ttttattgag ggatcgaaat tcttccaata
     3961 gttctgtgac attttcctga tagtttttca gtggcttttt aaaggaattg accgttaacc
     4021 cgaataaatt attttggata actttcatca aatcattacc gccaacagtc agtgtgataa
     4081 aatcagctgt tttgatattg tttcgaattt cttcttttgt ttttactcgc tttaagatct
     4141 ggtcacttcg ttcaccattt acaccatagt tttctgtttc aaccgcggtc aaattataac
     4201 ggtcttttag atcatttgcg acaaggggaa cgaatcctcc ttgcttggtt tgatctccta
     4261 ctccttcagt cagcgaatct ccgatagcta tgtagtgaag tttttctttt ttatttggat
     4321 cttctctcac agtagttact ggtttcatta gtgggccagc tttcggtata gaagcagcta
     4381 aacctgcata gatgatgatc gcaattaata atgggaacaa aacgaggatc agatgtcgtt
     4441 tcgttaattt cattttttca cctcttgtat tcgattggtt ctaatgttat caaaaatttt
     4501 atcaaaaagg tagtctttat gaaaaaagaa aacaattatt ttcctaattt tataaagaaa
     4561 aagagaataa gctgcaagcc tttcaggctg agggcttatc ctcttttcga ctaatctaca
     4621 taatacatga ttgcaaacgc gtctttacca gtatgcgtag cgataaccgg attcgtatgc
     4681 agtacaggga tatgcatgtc cgggaataat tcttgcaaac cctctttgaa ttcttgcgcc
     4741 aattcttttg catccgcatg tgaaataccg atttgtcgca catttggcaa ttctttcagt
     4801 tcatttttca aattattaaa ccatttatcg aacgttttct ttcctcgccc cttagttaca
     4861 gggattaatt cagaatgggc gaaattcatc accacgcgta tgttcaaaac actagacagc
     4921 aaaccagttg ttcgactgat tcttccacct tttaccaagt tatctagtgt ggatattccg
     4981 atgtataatt ttgtattgtc ttgtactttt ttgacttctt caaggatttc ctctactgtt
     5041 ttaccttctt ttgctaattc agctgctcga attacctgaa aagataatcc ttggtcagtg
     5101 gtatcactgt cgataaccgt tactttcgtt tttgttagat tactggcttg tcttgctgct
     5161 tctactgttc cactcaagcc ttttgtcata tggatcgaaa tcacttcact gccgtcttct
     5221 cctagctcat catacatcga gataaattct ccaatcggtg gttggcttgt tttgggcaga
     5281 ttttttgctt ttgccatcat ttccataaat ctttcgccgt ctagttggtc atcatcggga
     5341 tatactgttc cgtcgatcat gatggatagc ggcatgacac gaatgcctaa agcatcgcgt
     5401 acattttttt ccatcgtaca tgaagaatct gttacgattt ttacatttgt cattattttc
     5461 tctctctctt tctttaacaa gatgccttat tcatgttaaa ctacatgtaa gacaatttct
     5521 atttttagta taacagacaa ataatgattt ttcattaatt acacctagta aaggagccgt
     5581 tttcatggaa aaaacgaaat ttaatcgaac gtatgaaata ttaaatgaag tgtttaatgc
     5641 agtcacccat ggaattggga ctggattgag tatcgcggga ctagtggttc ttttagtaaa
     5701 aggtgcccac ttgcattcct ccctccatat tgtctcttat gctatctatg gatcgatgat
     5761 gattctttta tttttaacgt ctactttgtt tcattcattg atttttacaa aagcaaaaaa
     5821 ggtgttccaa gtattcgatc atagttcgat ttttcttttg attgcaggaa gttatactcc
     5881 tttttgtttg ctgagtatcc gaggatggct aggttggact ttatttgtat taatctggtt
     5941 gtttgcgatt cttggcatta tatataaagc gttgacgttg cataaacggg agagagtatc
     6001 aaaagtatct acgatcatct atatttttat ggggtggctc tgtgtcgtgg ctgctgttcc
     6061 tctttatcac tctcttggaa tgactggtgt tattttgatg gtagcaggcg ggctttctta
     6121 cacgataggc gcattctttt atagcttgaa ttctatccgc tatatgcatg ttgtttggca
     6181 tttgtttgtt atgttaggtg caggttttat gtacttctct gttttattct ttacttaata
     6241 aaaaaaaata aagctatgga tgggacagct ctaaataaca gctagtctat ccatagcttt
     6301 tttatatcgt caattcgcct gtcccgcttt ttacaggatt ctcattacgc tgatacgttt
     6361 caaattgatg cggataacga tttttttcat ctatttctcc ttgactagta ttgatcagtg
     6421 accaatcaga ccagtcaaca ggtggaaaat aagcatctcc atcgaataca tgatggatga
     6481 ctgtacggta taatatcgta caaaatggca gaaattcttg ataaacagct gaccctccag
     6541 cgatgaaaaa gataccgtct gtttcttttt ccagagcaag cacgtcttcc acactatgca
     6601 tgacatgcac gccttctgct ttataggaag aatctctcgt caaaacaatc gtctgacgat
     6661 tcggcaacgg acggctaccc atcccttcga aagtttttcg tcccatcact agtgtatttg
     6721 cttctgtcat ttgtttgaag aatttcagat catttggcaa acgccaagga agacggtctt
     6781 cttttccaat caatccgttt tcatcctgtg cccaaattgc agctaacatt ttctcatcct
     6841 ttctgattct aaacagcgat aggagctttg atcgccggat gtgggtcata tccttctaat
     6901 tggatatctt ccatctcaaa atcaaatact gactgctttt cctgattcaa aactaaagtc
     6961 ggaaatgcgc gtatttctcg gctcaattgt tctttcattt gttcgatatg attgatataa
     7021 agatgagcgt ctcctaatgt gtgaacaaat tcgccaaccg ctaaacctgt ttcatgtgca
     7081 attaggtgag tcagcaacgc ataacttgca atgttgaacg gaacgcctag aaacacatcc
     7141 ccactgcgtt gatacaattg gcagcttagc cgtccctctt gaacataaaa ttgaaacatc
     7201 gtatgacaag gaggcaaagc catgctagga acatcttccg gattccatgc agaaacgatc
     7261 aatcttctag aatcaggagt ctttttgatt gcttcgatca cttctttgat ttgatcgata
     7321 aagccaccgt cttttgtttc ccagtgacgc cattgatagc cgtagatgtg tccaagatcg
     7381 ccgtatgttt cagcaaagga atcatcggtt aggatcttct cgcaaaaaag cttgctctgc
     7441 tttttatatt cttcattgaa ttcaggatct gtcaaacatc tatggccaaa atcagtcata
     7501 tctggtccat tatagtctgg acttttgatg tagcgttcaa atgcccattc atcccaaata
     7561 tggtttttat tttgtaatag atagcggata ttcgtatctc cttttaaaaa ccaaagcaat
     7621 tcgcttttga tcaatccaaa aggtactcgt ttcgttgtta acaaaggaaa ccctttttgc
     7681 agatcaaacc gcatttggta tccgaataag cttaatgtgc ctgtgcctgt tctgtcttct
     7741 ttacgatgcc cttcttccaa caattttctt cctaagtcaa gatacgcttg ttccatcctt
     7801 tttcctccta atcttctacc cattcactca agtattccca ccgtgccatt ttttcttcaa
     7861 gttgtgcttc tgtttcagat acgtcgtttt gcaattcttg taatcgagtg aaattatctc
     7921 cttgatgatt catctcttct tggagcaatg agattttttc ttcaagttct gcaatctcat
     7981 cttcaatcgt ttcccattct ttttgttcca tgtaagataa tttcttcttt tcagcaggtt
     8041 ccttgctctg tatttttggt ttcgcttttt cagcaggctg ctgggtctct ttttgcttca
     8101 gtaaataact ggacatgttt ccgtaataaa gttcttgttt tccgtttcct tggaatacta
     8161 ataacttatc tgctaccttg tccaagaaat aacggtcatg ggaaacagtg atcacagcac
     8221 ctggaaaact tctaaaataa tcttccaaaa tcgtcaatgt tgctatgtcc aaatcattgg
     8281 tcggttcatc caataataaa acattaggtt gctggatcag cagtttcaac aaaaataacc
     8341 ggcgtttttc tccgcctgaa agtttgccta tcagtgtccc atgcataaat cttggaaata
     8401 aaaatcgttc aagtaattct gctactccga tttgactgcc atctgctgtt tttgcttctt
     8461 cagcagcttc ttgtaagtaa gcaatcatcc gtttatctgg tgccatttct tcattttctt
     8521 gtgtataata cgcaagatga accgtttcac cgatagacat tgtaccgcta tccaggggaa
     8581 ttctgccagc tagaatatta agcaatgtcg attttcccgc accattttta ccagtaatac
     8641 ctagtctttc ccgagattga atcagtaaat ctaaatgttc tagcagtgtt tggtcattta
     8701 tacggtaact gccatctttg atttccagta cttttttccc taaacgttgt gtcgctaggt
     8761 tcagctctaa atcatcttct tggtttactt ggtacaagtt ctcctttaga tcttcgaaac
     8821 ggttgatccg tgcttgctgc ttggtgccac gtgcctgtac gccagccctc atccaagcta
     8881 attcctgttt ataaagccgc ttgcgctttt cttcttgttc tacttctaca cgatcacgtt
     8941 ctgctttttc taagacgtaa gcttcataat ttcctttata ctcgtataat tttccaaatg
     9001 aaagttcgaa gattcggttt gctactctat ctagaaaata acgatcgtgg gtaaccatca
     9061 atagtgcgcc acggtattgt ttcaaatagt tttccaacca ctcgatcgct tcataatcta
     9121 aatggtttgt tggttcgtct agtaatagta aatcgggttc atcaatcaac acttgcgcca
     9181 gactaactcg ttttttctga ccacctgata aagtactgat ttttttatct aattcactga
     9241 ttcctaattt ttgcaaaata atctttgcgt tggtatctgt agtccaagca tcttctttat
     9301 tcatttgttc ttctgcttta gcataacgtt tttgcacatc agggtcttct ccgttttccc
     9361 ctagttcgat caatgcttcc tcatatgctc gaacagtttg gatcaagggg ctgtttcctt
     9421 gaaagactgc ttggagaaca gttaattcat cggaaaattc agctgcttga gaaagataac
     9481 caatacggta atcagttgga taaaacactg tttgccgatc accgtcgccg ctatcgatac
     9541 cggcaataat atttaagaga cttgttttac ccgtcccgtt cgtcccaatc aacccgatac
     9601 ggtctttttc gtggaccaaa aaagaaatct ggtcaaacaa atctttttca ccataagttt
     9661 ttttgaggtc agttactttt aattctttca acatgttccc tccttatctt ggatattgta
     9721 tcataaaaat agtgaacttc aggaaaattt ttttggtatt tgttcttttt gtgttctcaa
     9781 aaaaagaaca ggaccgattc gatcctgttc tgatatgttt tgttttttat aaaacttgaa
     9841 aatttttacc tttttatttt attggactcg tttttttgac tttgctcgaa acgcaaatcg
     9901 gaaagaaaag ttatcagatg gtttgttcat cgtataagat tctaacaaat tgatattcct
     9961 gcgatagctg ttcgcagaaa acgtcgtgat tttctcagca acttcgtatt catcaatgat
    10021 ttttctttca agataataac ctcttagcat tttttttgta aacagactat cttgctggat
    10081 catccacgaa gaaatcaaat tccccgagtt catttttatt ccttcaagtt tgcgttgctg
    10141 gataaaaaat tgaaccatcg tttcatcata aatatcagtc aagttatcta aactttcaat
    10201 aatcaactga gtattttttt gatatgtctc tctgatttct tggatatctt catcactaat
    10261 aaatgtctga cgatcagcat gtcttctttg ccaaaacatt ttcggatgga gaacgattcg
    10321 cattaaccta cggacaacaa tgaaccaaaa gccgataaaa gacaaaatct ggcttgtgat
    10381 agaatgttcg aatcgagaaa gaaaacggga gtaaaaacga taaccattag aagaaagctt
    10441 gcccgaacga taactttcat ccaatccatc ccgttcgatc gacaaaatca gtgcttgtat
    10501 ttcttgtact tcttgctttt catcatcggt taaatcttcc aagtacagat cacgtatccg
    10561 ttcttgataa ttttctatca ctgcttcagt ggctaaaaat tcttttgtat tacgatcaat
    10621 ttcctttatt tcttttcgca aaacttcaac gacctcttct aaaatcatca aagcattcaa
    10681 atccgtgctt ttcgcttcct ctgtttccgt cagcataggc aagacaatga tccctatgac
    10741 caaagtcact aagatgacac ccgccgtcag aaacaaaagc aaagaccgct ggtaaaacat
    10801 catattattg attgaaaatg gcaaaataaa aatcgtagcc aaactgactg ttccctttac
    10861 accgccaaat gttaagatca agatctcgtt caactgtttt ttgaactttt tactgccatc
    10921 tttgaataca taaaaaagac tgatagaaat gaatctgatc acaaaaagca tgacagagat
    10981 taatacgata atcgctaata aaagaccgtt tggatataaa ccgtctcccc atactggcga
    11041 gaacacttgc gtcaattcga ttcctaaaaa tataaatact aatgcattca gtgtaaaagc
    11101 aatagttgtc catgtactgt gggacaagct cgacaattct gcatcaaaca cagagatttt
    11161 cctaaaacca gaagcttgga gaatacctgc tgccactgca gcaatgattc cagatacatc
    11221 aaatgcctct gccaatacat aggctagaaa aggaagcaaa agttcgatca gcagataccc
    11281 tgtgacatct tgtgctgatg ctttttcgat caaatgaatt attttttgtt tgaaccagac
    11341 aagcaaaaag ccgatcaaag ctcccccaat actagaaact actagactca tccccgcgtt
    11401 tactgctgaa aaagatccgg tgatcagcgc accaagagca aactggaagg cagtaacacc
    11461 agatgcatca ttcaaaagac cttctccttc caaaatatgc atcgcctttt tagggatatt
    11521 cactcgtcca gaaagagaac tgactgctac cgcatctgtc ggacctaacg ctgctccaaa
    11581 agcaaagcag gcagcaagtg ggacacttgg caacaggaat gataacgtcg cacctacccc
    11641 gacaagggtc aaaatgactc ctccaaatgc caagaacaaa attgtcccga aatttttgag
    11701 aatcgaagaa atatctgctt tttctccttc tctaaataac aatggtgcaa taatcataac
    11761 taagaacaat tcaggttcaa aatcgattga ttgtccccac tctgtcagac ctaataccac
    11821 tccaaggaaa atttggatca gtggagctgg aatcattggc aaaatgcggc tagctatgtt
    11881 cgataaggtg atcgttactg caaatacaat aactaactca gcaaattcca tcgtccatcc
    11941 cctttatttt ttattttgtg taagcatttt taaaaattgt tcttttttct ttaaaatttt
    12001 ggtttcgact acttcaacct gtgtttggtt accctctaat tcacactgta cacgttttga
    12061 ctccaaacga agcagatcat gtttcagacg ttccaactga cgatacatca atatatttct
    12121 tttacgcaac ttacaaagta tttgacggcg gtcatccatc gaaagggcgc catagcgatc
    12181 atgcaacagt tttttttgtt gttcaaaact ctctctcatt ttccagatct cctatacaca
    12241 aacttccatc attatagcaa agttcttttt ggatgaaaaa tatttcattg tttcatcgtt
    12301 tattgctagt tagaaatttc cataaatttt tcaacgcctc attctattcc aaatagtatc
    12361 agatatcaag taaaacttaa gaaagataaa agtttctgac aaaagaatgt ttacttttcc
    12421 attcctgcct ttcttgttct tttatcacaa caaaaaaatg gggctgcgac aaaaatcttg
    12481 tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt agtaataaga
    12541 cacatctctt tcacagcttc atttttgcgt aaaaatcgat ttattcgaaa aattccccgt
    12601 atacatcttc tacacgttga tctggattga tcacgatata caagttacct gttttttcgc
    12661 tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct agatatggga
    12721 agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg atatcttcgt
    12781 atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata tcatgtgtcg
    12841 caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga tggatttcaa
    12901 caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc caaagatcgc
    12961 cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat tttagcgctg
    13021 tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg tactacctca
    13081 gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa tgacgtatct
    13141 gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt taaatcaatt
    13201 tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt tgttctacga
    13261 cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt tttccagttt
    13321 cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata ggtaaacgat
    13381 cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc atcaattctt
    13441 ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc aaagctccag
    13501 tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt tggttagaac
    13561 acttccacga tttcaaaaag gaacgaatct cattttcttt tagacccagt tggctgatca
    13621 ataatgtcca tacttggctc tcttctttca atgggacgga tggtaattca gcaaaacgta
    13681 aaagtgcttc gccttcttct ttcaaactgg gacagcaaat gtagcactct gtttcaataa
    13741 atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat tccacattta
    13801 tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg tagaaggttt
    13861 cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc attcgtaagg
    13921 catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt tcttctaaat
    13981 cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca aacgcattga
    14041 tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg acatgatctg
    14101 gtctgcggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca tattgttcat
    14161 cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt tttggaaaaa
    14221 gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg attggctggt
    14281 tcagcaagat atcacgtaca cttccaccta caaaataagc ttcatatcct gcattctcta
    14341 attttttcag aactggaatt gccttttggt attccagagg taattgtttt aatttcattt
    14401 ttccaacctt tcctgcgtgt ttcttgtact gagataaaga ctcagttgca tggtgactgt
    14461 ttgacaagtc cgtttcagag tcttcaaggc cagaaccact tcatcttgtt tactcgtcct
    14521 tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt tcaaaaactt
    14581 cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa acatcaccaa
    14641 gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct ccataagtgt
    14701 ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc tgagctaatg
    14761 gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc tgcatactgt
    14821 ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa aactttaacg
    14881 caaatcctag ataagagcag ttgagaaaat cgaatttcca tgctacattt agaaaggaat
    14941 ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata tggtcaaacg
    15001 aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg catttggtct
    15061 tgtttacttg aacatcgcta atcatttagc agaagggggc gtctcaggga tcaccttgat
    15121 cttaagagct ttattcggaa ttgatccagc ttatacgact ttattaatca acatcccatt
    15181 gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatacag ttctaggtac
    15241 actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa tcaatttaca
    15301 gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag gaagcgggat
    15361 cgtctaccgc atgggtggaa caacaggtgg cagcgatatt attgctcgga ttttagaaca
    15421 aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg tcttacttgc
    15481 ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt cgtatgtttt
    15541 cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc tacttgtcgt
    15601 ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa gaggtgtgac
    15661 ttttctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct atatggtcgt
    15721 tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg aaaaagcttt
    15781 cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt tgaagccaca
    15841 gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga ggaaagattc
    15901 cacatccagc tgatctttgt cttttttagt actatactat ttttaactaa ttgttatgtt
    15961 cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta acttcatcag
    16021 ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt tgagcataaa
    16081 gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt aattcagtat
    16141 cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag atagatagat
    16201 attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc tttttccaat
    16261 tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc tgcaaaaaca
    16321 atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga tccgcaaaat
    16381 tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca agataattca
    16441 agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac gttaaaaaat
    16501 tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt catctaataa
    16561 tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata attcttgcgc
    16621 tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga aatctggttc
    16681 atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa agtcttctaa
    16741 ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt cttccatctg
    16801 attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat tgcttaaagt
    16861 aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa gttcttctgc
    16921 tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa gttcgacttg
    16981 gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa tcatttcttc
    17041 ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat taacttctcg
    17101 aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat tcgtaaaagc
    17161 tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta aaggtgcgat
    17221 agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct gcatcgagat
    17281 tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg ctgcctcttt
    17341 gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg gttcatcagg
    17401 aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc cgataacttg
    17461 gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct tatccacttt
    17521 ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt ctgctaacgg
    17581 gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa tctgttttgc
    17641 ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata tatctgctgg
    17701 gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt catctgataa
    17761 agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc tcactgacga
    17821 tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggacgt tggtatccgt
    17881 ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc aaaaaaagaa
    17941 ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga tagccaagca
    18001 agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct tggagattcc
    18061 acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt agatggccat
    18121 tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg ttttctgcct
    18181 ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga gtcgttaatt
    18241 gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca caaatcattc
    18301 cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacttgccgc tcgctgattg
    18361 tttgatcata aacgatgcgt tctggcagat tgatggtttg cagtactaca cgatttggta
    18421 gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc gatacaggga
    18481 tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc agatccatga
    18541 tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag cgtttgacga
    18601 aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca caaatatacc
    18661 agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac aaatcataat
    18721 agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt agctggctga
    18781 ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa tcaagttcca
    18841 tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc tagaaaagat
    18901 aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa tacatgtgtt
    18961 ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt ttctgctagg
    19021 aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt
    19081 taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt
    19141 cgatttcttc accagtttgt gggttgcgac ctttgcgagc tgcgcgttcg cgaacttcaa
    19201 agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg
    19261 aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa
    19321 ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata
    19381 tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat
    19441 tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt
    19501 taccaatttt cacatacttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc
    19561 ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg
    19621 gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata
    19681 agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc
    19741 gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat
    19801 tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt
    19861 catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga
    19921 aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa
    19981 atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga cgataataat
    20041 tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc
    20101 ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat
    20161 cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc
    20221 gatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt
    20281 cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga
    20341 ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc
    20401 ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc
    20461 atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc
    20521 gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt tattacttct
    20581 gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac
    20641 acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat
    20701 aaagggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa
    20761 ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga
    20821 gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac
    20881 gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag
    20941 tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat
    21001 tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc
    21061 atctcagtca cagatacaaa atcaattat
//
LOCUS       DAZUMO010000046        20503 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000046 DAZUMO010000000
VERSION     DAZUMO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.046"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..1876
                     /locus_tag="ACG8ER_001800"
     CDS             236..1876
                     /locus_tag="ACG8ER_001800"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS5050377.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2030..3352
                     /locus_tag="ACG8ER_001801"
     CDS             2030..3352
                     /locus_tag="ACG8ER_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS5050378.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3414..3845)
                     /locus_tag="ACG8ER_001802"
     CDS             complement(3414..3845)
                     /locus_tag="ACG8ER_001802"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5050379.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3909..4745)
                     /locus_tag="ACG8ER_001803"
     CDS             complement(3909..4745)
                     /locus_tag="ACG8ER_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS5050380.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4935..5237)
                     /locus_tag="ACG8ER_001804"
     CDS             complement(4935..5237)
                     /locus_tag="ACG8ER_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS5050381.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="ACG8ER_001805"
     CDS             complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="ACG8ER_001805"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS5050382.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5691..5957)
                     /locus_tag="ACG8ER_001806"
     CDS             complement(5691..5957)
                     /locus_tag="ACG8ER_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS5050383.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6211..6936)
                     /locus_tag="ACG8ER_001807"
     CDS             complement(6211..6936)
                     /locus_tag="ACG8ER_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5050384.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7302..7372)
                     /locus_tag="ACG8ER_001808"
     tRNA            complement(7302..7372)
                     /locus_tag="ACG8ER_001808"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7338..7340),aa:Gly,seq:ccc)
     gene            7489..8241
                     /locus_tag="ACG8ER_001809"
     CDS             7489..8241
                     /locus_tag="ACG8ER_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5050385.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8257..8736
                     /locus_tag="ACG8ER_001810"
     CDS             8257..8736
                     /locus_tag="ACG8ER_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5050386.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8987..10567)
                     /locus_tag="ACG8ER_001811"
     CDS             complement(8987..10567)
                     /locus_tag="ACG8ER_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS5050387.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10560..11303)
                     /locus_tag="ACG8ER_001812"
     CDS             complement(10560..11303)
                     /locus_tag="ACG8ER_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050388.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11479..12255)
                     /locus_tag="ACG8ER_001813"
     CDS             complement(11479..12255)
                     /locus_tag="ACG8ER_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5050389.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12503..13339)
                     /locus_tag="ACG8ER_001814"
     CDS             complement(12503..13339)
                     /locus_tag="ACG8ER_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS5050390.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13350..14279)
                     /locus_tag="ACG8ER_001815"
     CDS             complement(13350..14279)
                     /locus_tag="ACG8ER_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS5050391.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14471..14824
                     /locus_tag="ACG8ER_001816"
     CDS             14471..14824
                     /locus_tag="ACG8ER_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5050392.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14898..15554)
                     /locus_tag="ACG8ER_001817"
     CDS             complement(14898..15554)
                     /locus_tag="ACG8ER_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5050393.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15780..17714
                     /locus_tag="ACG8ER_001818"
     CDS             15780..17714
                     /locus_tag="ACG8ER_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5050394.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17800..19023)
                     /locus_tag="ACG8ER_001819"
     CDS             complement(17800..19023)
                     /locus_tag="ACG8ER_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5050395.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19017..>20503)
                     /locus_tag="ACG8ER_001820"
     CDS             complement(19017..>20503)
                     /locus_tag="ACG8ER_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS5050396.1"
                     /translation="SQIIHWFSQIFEYECADGNWQFFKIIVGTIIQKFGYPVKESALK
                     EAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPEKPISQIYKNRAID
                     FSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKLGEIKGLVNRNIRW
                     WMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLALPEDHEYWTAKEM
                     PMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKYLKFAYSSQFGFSI
                     PRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNNVRDIVCKKEVLAS
                     TWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECSPEKEGIIIEEDKE
                     NYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPGLEGTFGKGTHWLG
                     TGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
BASE COUNT         6355 a         4087 c         3536 g         6525 t
ORIGIN      
        1 tctatttatc tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga
       61 gatacgcata gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg
      121 atttcttttg actctattac tcatataaat aaaatttatt atacctgctc tttttgacta
      181 tatcctcatc tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct
      241 tacagggcta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
      301 agaacgggca aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca
      361 acaatctgtc caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat
      421 cttcttaatg aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca
      481 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
      541 caacgaaatt ttcttgaaac cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa
      601 tatcaaagca agtttgaaaa acgcagcaga tggacgcgac attcgtttga tcgttgtgac
      661 agatgcagaa ggaatcttag gtatgggtga ctggggtgtg aacggtgttg acatcgctat
      721 cggtaaattg atggtctaca ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt
      781 atcaatcgac gctggtacta ataacgaaga attattgaat aatccattat acttagggaa
      841 tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc attgatcaat tcgttcaatc
      901 agctactgaa ttattcccag aattgttatt acattgggaa gattttggac gaggaaatgc
      961 tgcaacgatc ttagaaaaat acgaagataa aatcactact ttcaatgatg atatccaagg
     1021 cacaggaatc gttgtactag ctggtgtatt aggtggattg aatatatcag gtgagcaatt
     1081 gaaagatcaa acgatcttga catttggtgc tggtactgct ggtgtaggta tcgcaaacat
     1141 ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
     1201 tcaagtagat aaacagggtt tattgtttga agatacaaaa ggattgactc ctggtcaaat
     1261 cccattcgct cgtaaacgct cagaattcac taatgctgat gaattgacaa acttagaagc
     1321 agttgtaaaa gcaatccatc caacgatcat gatcggtact tctacccaac ctggagcatt
     1381 cactgaagaa atcgtgaaag aaatggcggc tcatacccca cgtccaatca tcatgccttt
     1441 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttgattgaat ggacggacgg
     1501 aaaagcttta gttggaacag gtatccctgc tgctgatgtg gaatataacg gtgtaactta
     1561 ccaaatcggt caagcaaata atgcgttgat gtacccaggt ttagggcttg gtttgattgc
     1621 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
     1681 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
     1741 attctctcaa acgattgctg aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg
     1801 tgaagaaatc acggatatca aagaagctgt tgaatctgct aaatgggttc ctgaatataa
     1861 atctttagaa gattaagatt gttatagtaa aagaacatac agaaactttg tatgacgtct
     1921 aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca actactgctc ttttgccgct
     1981 tctctttggc ttttttcata cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
     2041 aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat
     2101 taccggcgta agtttgcccc tttatttgat catggtcctt gtcctgatca ttgcaatggc
     2161 tttaggaaaa ttaccgacga atatgattgg cccaatcgct gttttggtca ttttcggtaa
     2221 tttatttcat tttatcggaa caaaaatccc tatagtcaaa agttatttag gtggtggctc
     2281 cgtttttgcg atctttgcct ctgccgcgat tgctacattt gggatcttac ctgagtatgt
     2341 agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt ttagactttt atattgctgc
     2401 cttgattact ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg
     2461 ttttattccc gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtagg
     2521 tatgcttatc ggaaatggtt ttgcaaattc cgttttatat atctcattgc ccgcaatggc
     2581 tggtggtgtt ggagccggag ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac
     2641 ggacgcttct tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc
     2701 tatcattggt gcagcgttag ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa
     2761 tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc
     2821 cattccacaa ttaggtgtcg gtttgatggt atcattgact ttcttcatct taggacaaat
     2881 ctgtaactat tttgttccta aagtccatgc ttacgcattc atgatcatta tcgttgttat
     2941 ctgtaaaatc gcaaacctat taccagaata ttacgaagat gcagctatca tgttcaacaa
     3001 cttgattgtc aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttattgaa
     3061 tctaaatgta ttggcttctg ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt
     3121 tatcgcgatt tcagtagttt caggatttgt cggtcgtttg tttggtttat atccaatcga
     3181 atcaactatt actgctggct tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt
     3241 cttatctgca gctaatcgga tggaacttat tgcttttgct caaatgggca atcggcttgg
     3301 tggtgccatc gttttgatca tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa
     3361 aatacctgat catcaagtat cggcttgatg accaggtatt ttttgataaa aatttacagg
     3421 attttcaaaa atcttttttg cattttatcg gaatgataac cgatgcgttc ataaaatcgg
     3481 tgtgcttcta ctctgtattc tgcagaatta agtcttattg ctgatattcc tcgtcttttc
     3541 gcttcttcta cgccagtcat caaggccttt ccgattcctc ttttttcagc atgtttagac
     3601 acagcaagtg ccataatatt gaacatcgga tcgctgaaaa tcgattcata ggactccgct
     3661 tgtacatagc cagctgcttt tttagaagta ggatcttcat agaccaagaa aaaatttttt
     3721 tctggctcct tcaacagttt cttcatttgt ctcaccgtaa cggttaatgg tacatcatat
     3781 cctaattgtt ccgcattgat ttctcgtagt gcttctgcat ctcctaaacg catttcccgc
     3841 aacatccaaa tcaccagcca atttttattt ttttaaatga atttttttat cacagtatac
     3901 tccttttttc acttaaaaaa agacttttca gtgattttga cttgcttgaa tccagatcct
     3961 tctaatgttt gcaagatgac gaaagcattg taaagattga tcaaaagaac cacgatgaca
     4021 atcgcttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
     4081 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
     4141 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
     4201 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
     4261 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
     4321 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
     4381 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
     4441 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
     4501 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
     4561 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
     4621 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
     4681 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
     4741 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
     4801 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
     4861 agtgctaaac gaaaaaaccg ggctctactt ttcagagagt ccagcttttc ttacacctta
     4921 tcgattcttt tgttctattc ttcttccgcc atgaatgtat tgaatacttc ttcgatcata
     4981 tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg
     5041 atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc tggatataaa
     5101 agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat
     5161 gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa
     5221 tggttatgtt ctgtcataag attacctctt ttctttttat tattaaaata attgcttacc
     5281 tgagccgtct aaataatttt gtaaaatcat cacagctgct aatttatcga tgactttttt
     5341 tcgtttcgct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
     5401 tagtcgttca tcttgataat caaccggcaa ggagaatagt tctttgatct tctctccata
     5461 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atattcttag gtaatccaac
     5521 tacaaaacgg tctacttcgt actgcgccac taattccttc aaccgatcaa aaccaaactc
     5581 ttcttctgcc tcattgattc gaatgatttc tactccttga gctgtccagc caaagggatc
     5641 gctcacagca acgcctactg tcttagaacc gacatccaag cccattactc tcataaattc
     5701 actccatgat ttgagaggta gtctttgaca attacttcca tgatttcatc gcgttcgtga
     5761 cggcgaatca aatttcgtgc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
     5821 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
     5881 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
     5941 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
     6001 tacaccctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttctgaa
     6061 taaattaaga aacacgattt gtctgatttt tccttttttc tgtggctttt tcaaaagccc
     6121 tctttccata aagtatacgc tatatttctt tgaaagtatg tttttacaca attttttcat
     6181 aaagttcata aaaaattcga attgctgcta ctaatttttc gttttcgttg cttgccctgt
     6241 ctcataattg atggtgtagc catcttgaac attgtagata aagacattga attcaatttt
     6301 tttgtcttca atgctttctg cttccaactg cacacctctt gctaccagtt catttccctc
     6361 gaaatgagga gtgacacgat agcgtacatg atgatttgtt tccttgatat atgccgcaat
     6421 atcattttcg tgggcaagca tgtacggagc attcaacgaa cgtgtccctg tcattaagtt
     6481 tttaatgtta ttattttgac cagtcaactg gaaaccaatc aaatggctgc ggttgtataa
     6541 ccattgtccg tcacttaatt tcttttgttt ccaacctgtc ggatcaatat acaaaggttc
     6601 tctcttttca gtaggaaaca agtcttttcc caacatcgca ttggcttgtc ctactctgtt
     6661 caatttgtct atatttgaat agctttccca tgtccctttg gaaagactaa gatcctcttt
     6721 tgtaaactcc ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg
     6781 cacatccgct acttgagaag cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa
     6841 gatatctgaa tctttttgtg tggtggaaat accgagtgcc cctgcaatca cagcagcgac
     6901 gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagtt
     6961 ccctaaatct taacatatgt tcccctataa ataaatactg gaaagaaata gaaacagtaa
     7021 aaaaataaaa ccagttttta gacggcttaa gaatacaaaa aaagagacgg attcatcgtc
     7081 tctactcaat cacaaaacac tatttacaag gtatttatta tcattcaaaa aatttttctt
     7141 aatactcaaa actaaacggc ttctgtacga cctcttggta aacagtgttc aaaagcttgc
     7201 atctgcggta ataagtccaa ccaagtcaaa aacataagaa gcttttttgc ttgactattc
     7261 ttattacttg atgcagggca gctttttcac agcctctttt aagcgggtga cgggaatcga
     7321 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcgatacaaa
     7381 aacagtatac gcctctttca ctccttcctc aagttttttt attctttacg cttccgatat
     7441 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     7501 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     7561 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     7621 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     7681 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     7741 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     7801 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     7861 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     7921 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     7981 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     8041 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     8101 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     8161 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     8221 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     8281 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     8341 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     8401 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     8461 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     8521 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     8581 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     8641 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     8701 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     8761 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     8821 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
     8881 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
     8941 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
     9001 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
     9061 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
     9121 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
     9181 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
     9241 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
     9301 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
     9361 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
     9421 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
     9481 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
     9541 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
     9601 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
     9661 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
     9721 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
     9781 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
     9841 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
     9901 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
     9961 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    10021 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    10081 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    10141 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    10201 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    10261 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    10321 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    10381 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    10441 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    10501 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    10561 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    10621 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    10681 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    10741 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    10801 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    10861 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    10921 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    10981 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    11041 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    11101 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    11161 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    11221 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    11281 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    11341 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    11401 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    11461 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    11521 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    11581 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    11641 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    11701 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    11761 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    11821 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    11881 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    11941 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    12001 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    12061 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    12121 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    12181 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    12241 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    12301 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    12361 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    12421 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    12481 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    12541 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    12601 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    12661 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    12721 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    12781 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    12841 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    12901 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    12961 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    13021 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    13081 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    13141 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    13201 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    13261 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    13321 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    13381 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    13441 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    13501 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    13561 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    13621 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    13681 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    13741 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    13801 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    13861 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    13921 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    13981 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    14041 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    14101 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    14161 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    14221 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    14281 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    14341 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    14401 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    14461 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    14521 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    14581 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    14641 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    14701 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    14761 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    14821 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    14881 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    14941 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    15001 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    15061 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    15121 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    15181 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    15241 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    15301 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    15361 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    15421 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    15481 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    15541 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    15601 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    15661 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    15721 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    15781 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    15841 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    15901 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    15961 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    16021 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    16081 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    16141 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    16201 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    16261 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    16321 aacaccctga cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt
    16381 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    16441 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    16501 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    16561 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    16621 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    16681 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    16741 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    16801 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    16861 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    16921 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    16981 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    17041 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    17101 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
    17161 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    17221 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    17281 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    17341 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    17401 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    17461 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    17521 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    17581 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    17641 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    17701 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    17761 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    17821 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    17881 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    17941 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    18001 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    18061 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    18121 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    18181 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    18241 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    18301 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    18361 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    18421 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    18481 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    18541 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    18601 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    18661 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    18721 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    18781 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    18841 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    18901 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    18961 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    19021 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    19081 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    19141 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    19201 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    19261 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    19321 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    19381 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    19441 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    19501 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    19561 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    19621 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    19681 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    19741 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    19801 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    19861 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    19921 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    19981 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    20041 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    20101 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    20161 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    20221 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    20281 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    20341 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    20401 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    20461 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttt
//
LOCUS       DAZUMO010000047        20333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000047 DAZUMO010000000
VERSION     DAZUMO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.047"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1532
                     /locus_tag="ACG8ER_001821"
     CDS             129..1532
                     /locus_tag="ACG8ER_001821"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5050397.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            1532..2965
                     /locus_tag="ACG8ER_001822"
     CDS             1532..2965
                     /locus_tag="ACG8ER_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5050398.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            3110..4036
                     /gene="trxB"
                     /locus_tag="ACG8ER_001823"
     CDS             3110..4036
                     /gene="trxB"
                     /locus_tag="ACG8ER_001823"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS5050399.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            4250..5977
                     /locus_tag="ACG8ER_001824"
     CDS             4250..5977
                     /locus_tag="ACG8ER_001824"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS5050400.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            5992..6342
                     /locus_tag="ACG8ER_001825"
     CDS             5992..6342
                     /locus_tag="ACG8ER_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5050401.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(6434..7294)
                     /locus_tag="ACG8ER_001826"
     CDS             complement(6434..7294)
                     /locus_tag="ACG8ER_001826"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS5050402.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            7386..7814
                     /locus_tag="ACG8ER_001827"
     CDS             7386..7814
                     /locus_tag="ACG8ER_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5050403.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            7977..8153
                     /gene="rpsU"
                     /locus_tag="ACG8ER_001828"
     CDS             7977..8153
                     /gene="rpsU"
                     /locus_tag="ACG8ER_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS5050404.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            8180..8626
                     /locus_tag="ACG8ER_001829"
     CDS             8180..8626
                     /locus_tag="ACG8ER_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS5050405.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(8780..10159)
                     /gene="brnQ"
                     /locus_tag="ACG8ER_001830"
     CDS             complement(8780..10159)
                     /gene="brnQ"
                     /locus_tag="ACG8ER_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5050406.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            10865..11836
                     /locus_tag="ACG8ER_001831"
     CDS             10865..11836
                     /locus_tag="ACG8ER_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS5050407.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            11860..14052
                     /locus_tag="ACG8ER_001832"
     CDS             11860..14052
                     /locus_tag="ACG8ER_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HFS5050408.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDKNIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            14069..14545
                     /gene="ybeY"
                     /locus_tag="ACG8ER_001833"
     CDS             14069..14545
                     /gene="ybeY"
                     /locus_tag="ACG8ER_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS5050409.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            14529..14924
                     /locus_tag="ACG8ER_001834"
     CDS             14529..14924
                     /locus_tag="ACG8ER_001834"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5050410.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            14939..15838
                     /gene="era"
                     /locus_tag="ACG8ER_001835"
     CDS             14939..15838
                     /gene="era"
                     /locus_tag="ACG8ER_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS5050411.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFTQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            15981..16793
                     /gene="recO"
                     /locus_tag="ACG8ER_001836"
     CDS             15981..16793
                     /gene="recO"
                     /locus_tag="ACG8ER_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS5050412.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            17177..18094
                     /gene="glyQ"
                     /locus_tag="ACG8ER_001837"
     CDS             17177..18094
                     /gene="glyQ"
                     /locus_tag="ACG8ER_001837"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS5050413.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            18096..20171
                     /gene="glyS"
                     /locus_tag="ACG8ER_001838"
     CDS             18096..20171
                     /gene="glyS"
                     /locus_tag="ACG8ER_001838"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS5050414.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGKASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         6848 a         3594 c         4377 g         5514 t
ORIGIN      
        1 agatatttac aaaaatccac tagaaaacag taaattttgt aaatgttact ggaaagaaac
       61 agatttctat tgtttgtttt gtttacaaaa aagataatga aagtgtaaaa aagtaaagga
      121 gtgagaacat ggcaaaagga atcaaaatcg ctacgattgg tggaggcagc agttacaccc
      181 ccgagttgat ggaaggattt atcaaaagat atgaggaatt accgatacga gaaatctggc
      241 tcgtagatgt agaagaagga aaagaaaagc ttgagatcgt tagtcagatg tctcagcgta
      301 tgtgggatgc ttctccgtat gaagtcaaga tctatgcaac tctggatcgg gaagaagcat
      361 tgaaagatgc ggattttgtt acgactcagt ttcgtgtcgg tcaattagat gcacgaatca
      421 aagacgagcg gatccctagt tattatggga tgattgggca agaaacaaat ggtgctggcg
      481 ggatattcaa agcttttcgt accgttccag tcgttttggc aatcgttgag gacatgaagc
      541 gtttatgtcc aaatgcttgg ctgatcaatt tcgcgaaccc aagtgggatg gtgacagaag
      601 cagttatcag atatggcaaa tgggataaag tgattggatt atgcaatgtg ccagtgatgg
      661 cgatgatgac agaaccagca atgcttggca agaaaaaaga agagctgatc tacaagtttg
      721 ccggtctaaa tcatttccat tggcacaaag tagcagataa taaaggaaat gaccgcacgg
      781 cagaattgat tgatcgttta tacgatgaag agaatggatt accgaaaaat atctttgata
      841 tcccgttttt caaagaacaa ttaaaacaaa tgaacatgat cccttgtggc taccaccgct
      901 attactatcg tgaagaagaa atgctggaac atgctttaga agaatatcaa acgattggta
      961 caagagcgca gcaagtgaaa gaaacagaag cagagctatt tgaattatac aaagatccag
     1021 aattagacca taaacctgaa caattgcaaa aacgaggtgg cgcttattat tcagacgctg
     1081 cctgtgaaac gattgcttca atctatggaa ataaagaaac acagatcgtg gtatctacaa
     1141 agaatgatgg agctgttcct gacctgccgg cagattgtgt agtagaagtc actgcctatg
     1201 ttggcgcaca aggagcgaga aatgtagcgt ttggttctct tcctgctgct gaaagaggct
     1261 ggttgcaagt aatgaagaat atggaattgc tgacgatcga agcagctgtg actggtgatt
     1321 atgggacagc tttacaggcg tttacgatca atccacttat acgatcagga aagacagcaa
     1381 aacggatcat ggatgagttg tttattgcac accagcagta tttaccgcaa ttcaaggaga
     1441 caatcagccg attaaaagaa gaaggaatcg aagttcaaga tgaactggca agagaattat
     1501 caggtaatac acatataatg gaggaagtat aatgggattc agaaaagatt ttttatgggg
     1561 cggtgctaca gcagccaatc aatgtgaggg cggttatgat gaaggtggac gcggactagc
     1621 aaacgtagat gttgtgccaa tcggaaaaga ccgttttcct gttatcaccg gaaagaaaaa
     1681 aatgtttgaa tttgatgagg atcattttta tccagcaaaa attgcaatcg atatgtatca
     1741 tcgttataaa gaagatattg ctttgtttgc agaaatgggg ttcaagactt atcgaatgtc
     1801 catcgcctgg agtcgtattt ttccaatggg ggatgagaaa gagccgaatg aagctggctt
     1861 gaaattttat gaggatgcct ttaaagaatg ccaaaagcac ggaattgagc cacttgtgac
     1921 gattacgcat tttgattgtc caatgcattt gatcaaagaa tatggtgcgt ggcgttccag
     1981 agaactcgtt ggtttttatg aaaatctgtg tcgagtcatc ttcaatcgct ataaaggact
     2041 tgtcaaatat tggctgacat tcaatgagat caatatgatc cttcatgcac cattcatggg
     2101 cgcagggctg tattttgaaa aaggagaaaa tgaagaacaa atcaaatatc aagcggcaca
     2161 tcatgagttg gttgcaagtg ccatcgcaac gaagattgct catgaagtag atccggagaa
     2221 caaagtaggc tgtatgctgg cagcaggagc atattatcca tacacttgca atcctgaaga
     2281 tgtttgggct tctagaaaat cagatcgaga aaattatttc ttcattgatg tccagtcacg
     2341 aggagaatat cccgcttatg cattgaaaga aatggagcga aatggcattc agatcgaaat
     2401 ggaaccaggg gatgccgcat tgctgaaaga acatactgtt gattttatct ctttttctta
     2461 ttatgcttcc cgagtttcga ctaccgacaa cgaactcttg gaacagactg caggaaatat
     2521 ctttgcttct gtcaaaaatc catatctgca agcaagtgaa tgggggtggc aaattgatcc
     2581 attaggcttg agaataacga tgaatgatat ttatgatcgt tatcaaaaac cattgtttat
     2641 tgtagaaaac ggcttaggtg cagtcgatac gccagatgaa aatggttatg tggaagatga
     2701 ttaccgaatc gattacctgg cagcgcatat ccaggcgatg aaggatgcag tagaactaga
     2761 cggagtcgat ctattaggct atacgacttg gggatgtatt gatttagtat cagctggtac
     2821 cggtgagatg aagaaaaggt acggattcat ttatgtcgat cgtgataacc aaggaaatgg
     2881 cacattagcc cgttcaaaga aaaaatcatt caactggtat aaaaaagtaa ttgattcaaa
     2941 tggagaaaat ctttcgaacg attgatcaat gatttataaa agtctattgt ttttcagcgg
     3001 atttttcgag tataataaag ctattcgaga aacgcaagca gaacaataga ctttttttcg
     3061 cattggctgt aaagtatgct tacttgatta agtaaaggag aatctacata tgtatgatgt
     3121 aatcgttatt ggcgcaggtc ctgctgggat gactgctgct ttatatgctt cacgttcaaa
     3181 cttatccgta ttgatgatcg aacaaggtgc ccctggcggc caaatgaaca atacggcaga
     3241 aatcgaaaat tatcctggat tcgattcgat catgggcccc gatctagctt ataagatgta
     3301 tgaaagtgtc tcaagatttg ggacagaaaa tgcttatgga atcgttcaag atatcaagga
     3361 tcacggcagc cataaagaag tcatctgtga agataaaagt tacgaaggaa aaacagtcat
     3421 tatcgcaaca ggatgtatcc accgtaaatt aggcgtacct ggcgaggaag aatttgctgg
     3481 acgaggcgtt tcttactgtg cagtctgtga tggtgcgttt ttccgtaaca aacgtctagt
     3541 agtcatcggg ggtggggact cagcagtaga agaagccatc tatttgacac aatttgcttc
     3601 tgaagtagtg atcgtccacc gtagagatga attacgtgca caaaagatca tccaagaccg
     3661 tgcatttgca aatgaaaaaa tttcctttat gtgggataca gtcgttgacg aaatcgttgg
     3721 aaacgaaatg gtagtgacag gtgtgaaagc acgtaatgtc aaaaccggca atgaatttga
     3781 tttagcagca gacggagtat tcatctatgt tggactggat ccattaactg caccttttag
     3841 agaagcaggt ttgacgaacg aagctggctg gattccaaca gacaacgaaa tgaaaacgaa
     3901 tatgccaggc gtttttgcca tcggagatgt tcgtgaaaaa gatttgcgac aaatcactac
     3961 agctgttggt gacggcggta ttgctggaca acaagtgtat aaatacttgg agtctttaga
     4021 agaaacaaca gcataagttt aaaaagagta gaagaacggg ttcaattgtt cttttactct
     4081 tttttttcgc gtgaaattta ctgaatctga tttatgcgaa cacgtccaac accttttgat
     4141 aaaagaatcc tttcttgatt taagcgtttt ttggtcctca tactttcata tctaataagt
     4201 gaatgctata cttattaaga accatatttc agaaagaggt gttattttca tgtcttggga
     4261 acaagtgtat gaacaatggg tacatgaaga aaatttagaa gaaaacttaa aaaaacaatt
     4321 aaatgatctg ggtgaagatc ctgaaaaact tgaagatgct ttttatgcac cgttggaatt
     4381 cggtactgca ggtatgcggg gaatcttagg acctgggatc aatcgaatga acatctatac
     4441 ggtaagacaa gcaacagaag gactagcacg tttcatggat acacaaggtg aagaaacaaa
     4501 acgccgaggt gtcgctatcg cttatgactc acgccatcag tcaccagaat ttgcaatgga
     4561 agcagccaaa acactagcac atcacaatat cccgtcttat gtattcgaaa gtctgcgtcc
     4621 aacaccagag ctttcttttg cagttcgtca tttgaagacg tttgcgggaa tcatggtaac
     4681 cgcatctcac aacccagcgg cttacaatgg atataaagtc tacggagaag atggcggaca
     4741 aatgcctcct gcagacgcag atgcattgac gaaatatgtc cgtgaagtca gcaatccgct
     4801 aaaagtcgaa gtattatctg acgaagaagc taaacatagc gggttgatca cgatcatcgg
     4861 agaagaagtg gacgctgctt atctagaaga aatcaaagca gtgacgattg atcgtgaatt
     4921 ggttgaaaca atggggaaag atttgaaact tgtgtacacg ccacttcatg gaacagggaa
     4981 aatgttgggc gaacgtgcgt tgaaacaagc tggattcgaa caattcgtac tagttcccga
     5041 acaagctgtg gcagatccag atttcactac ggtcaaatcg ccaaatccag aagaacattc
     5101 agcttttgaa tatgctatac gacttggaga aaaagaagga gctgacttgc tgattgcaac
     5161 agatcctgat gcagaccgtt taggcgcagc ggttcgtttg ccagatggtt cttatgaagt
     5221 attgactggg aaccaaatcg gagcattgtt tgctcaatat attttggaag cacatcaatc
     5281 aagaggcact ttaccagaaa acgcagcggt attgaaatca atcgtttcca gcgaattgcc
     5341 tacagcaatt gcggaacact acggtgctac catgttcaat gtgttgacag ggtttaaatt
     5401 cattgctgaa aaaatccaac aatttgaaga agaccattca catactttta tgtttggatt
     5461 cgaagaaagt tatggttatt tagttaaacc ttttgttcgc gataaagacg cgatccaagc
     5521 attgctttta ttagcagaag tagcagctta ctataaaaaa caaggcaaaa cgttgtatga
     5581 cggcttgcaa acgatttttg aaacctatgg ctatttcgca gaaaaaacga tttctgtgac
     5641 aatgagcgga caagaaggtt ctgcgaaaat ctctgctttg atggaaaaat tccgtaaaga
     5701 agcaccgaca gaatttgccg gagcaaaagt tattcaaaca gaagacttta aagaattgac
     5761 gagaacagac cgtgaaggaa acgtagaaaa actaacaaca ccgccatccg acgtattgaa
     5821 gtatacattg gaagaaaatg gctggatcgc tatccgccct tcaggaactg agccgaaaat
     5881 caagttttat attggtgtaa aaggaagctc tacagaagac gcagccgaaa aagtagcctc
     5941 ttatgaagcg attatcaaaa agatgacaga agaataaaaa acttgagact gatggaaaaa
     6001 gtggtaaact ttagaagtca gtcttttttc aagttggagg tgaatggatt gccagacacg
     6061 caaaaatcaa aagtagagaa agtcagtcaa ttattcaaaa tgttgagtga tcccactcgt
     6121 cttaaaattc tgctatatct gaaagacggg gaacaaaatg tgacagcaat cacccaagca
     6181 gtggaaatgg agcagtctgc tgtatcgcat caactacgtt tattaagaga aaatcatgtc
     6241 gtcaagtctc atcgcgaggg aaaagcgatt ttgtatagtc ttgatgattc tcatgtactg
     6301 gatattttga accagacact caaacatgtg gaacatcaat aacgataggt gaaaatagat
     6361 aaacactgga gtgtgacaac gaagaaattt cgttgtcaca ctccagtgtt ttttaagaaa
     6421 aagacaaaaa tgattaatcg cttttttctg ttgtttcagt tccataataa ctatgatctt
     6481 ctaggtctaa agcattcaaa ataagcgaag ccgtttcttc aatagataaa gatgccacat
     6541 tgatcactac acatcctagt ttttcataaa gatcattcgc aaaatcgagt tcagctctaa
     6601 ttttttcgat atcggaataa gcagtatcag ggtttaatcc gtaagagcgc atccgttctt
     6661 tacggatatt atttaaaata tctgggttat tcgttagtcc cacgattttt ttaggatcga
     6721 tttcccacag ctgtttggga atatgtgctt ggggaatcaa aggtaaatta gcaactttta
     6781 aatttttatt tgctaaaaac aagctcaaag gcgttttgga agttcgggat acgcctagca
     6841 aaacgacatc tgcttctaaa aaaccacgag gatctttccc atcatcgtat tttacagcaa
     6901 attccattgc tttgatccgt ttgaaatagt tctcattcaa taaatggagg gcaccaggtt
     6961 gccgaattgg cttgatacca gttaaacgtt caatttctga tatcggtggc gtcatcacat
     7021 cgaaactaaa taattgatgt tcttcacaaa aagaacgtgt tatctcgatc agttcttgat
     7081 tgattaatgt atgcaggaca agtgcttttt cttctaatgc atcttgcaat gcctgcctta
     7141 atgttttttc atctgtcacg aacgttctgc ggaataggtt gaactcaacg gtaggatact
     7201 gagccatagt agcttgcgct agttttgtcg cagtttctcc agcagagtcg gagatgacaa
     7261 aaaaagtaac gatttctgta gaatcggaag acatggataa aacctcactt gcattttttt
     7321 tatgtttatt ataccatact ctagaatgcg tacttcgtaa tcattttgat ttggaggaat
     7381 aagtcatgaa aaaacagaca ttgatcgaac aatcattaga aacattgaaa aataacggtt
     7441 ttaaatatac taaaaaaaga gaagcattgt taacttattt agtgaagcgt aaccgctacg
     7501 tatctgctaa agaagttttt gactatatga atcaggaatt caaaggggta agttatgata
     7561 cgatttaccg gaatctccat gattttgcag agatcgattt actggaagaa accgatttaa
     7621 atggtgaaat gaagtttcgt tttcattgct gtcaggggga aatcggccat caccatcatt
     7681 ttatctgtac agtgtgcgga acaacgaaag aaatccatat gtgtccaatg aattatttcg
     7741 aagaacagct aggtggatgc acgatcgaag ggcatagatt cgagattttc ggaagatgtg
     7801 aaaattgtca ataaggattt aaataaaata acatttgata atttaaaaaa aaaagcaaga
     7861 tttccttgac ggtcaaaaca gcattagata taatatttac aggacagtct tgttttttga
     7921 gatatatttc aataacaatt acggttactt tttaagctcg gagggaggga attaacatgt
     7981 caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc tcttcgtcgc ttcaaacgtt
     8041 ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg tgaattctac gaaaaaccaa
     8101 gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg caaaaaattc tagttctgtg
     8161 atgaattgga gttggtagtg tgtcactact aagtagatta aacgatgaca tgaaagcagc
     8221 gatgaaagcg aaagataagg aatctttaca agtcatccga atgatcaagt catctataca
     8281 aaacgagcag attaaagaag gtcacgactt gacagaagaa gaagaactaa cggtgttgtc
     8341 tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt gaagaagcag gacgtgacga
     8401 tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa aaatatatgc cggaacaact
     8461 ttccgacgaa gaaattcgac aacttgttca agaagcaatc actcaaactg gtgcttcatc
     8521 aatgaaagaa tttggcaaag ttatgggtgc aattatgcct aaagtcaaag gaaaagcaga
     8581 tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa tcttaatttt atcatttaaa
     8641 ctgcggacag tgattgtccg cagttttttg tgttcgtaaa aaaagaacta aaaaacagct
     8701 agaagaaaga ataagattct ttcttctagc tgtttttttc ctgtttaata tctcttatgt
     8761 taaattgtgt ttgatttggt tattcttctg gtagttcatt tgcttcttgt ttataccctg
     8821 ataggcgata ggcgtaataa aagagtaagc tgccagcgta accaagtaac gttgggacca
     8881 tccagccaag tcctaatgca gcaaaaggaa cgtgatgatg ataaaatcct agtaagcttg
     8941 ctacgacagt ttgctgactc actaatgcag gcgaatttgc caacatatcc aatacggctg
     9001 gaacggcaga aaagaacatc gttacttgat agacaaatgg actcttttgg aagaattttg
     9061 ctgttaagga aagcaagatt aacgcaagtg acaatggata caaaagcatc aatactggta
     9121 aagaccattg gatgatatta tccaatccgg cattcgcaac gacaaaagag acaaaagtcg
     9181 ttaaccgcag ccatgtcata taactgactt tagggaacag tttatggaaa tcttgtgcaa
     9241 aagaaacaac gagtcccata gcggtagtga agacacctaa tgtgaccatt acccctaaga
     9301 agctagcacc gaagttacct aaataaaaat tagtgatctg agaaagtgca gttccgccat
     9361 tttctgataa agacatctta ttcaaactca tcgcaccgag aaggaccaga ccaaaataaa
     9421 tcatgacttc taacgcaacg ctcaggctac cagcttttgc tgttaattct gtgacttttt
     9481 tatctttaaa accaagatca cgaactgcat ggacaaaagt gacacttaaa gcaagtaaag
     9541 ctactgcatc aaccgtgttg tatccttcta aaacgccatt tgtcaaagcg ttcgtctgat
     9601 aagaatctgc agcagattgg tttagattcc ccatgggttg gaataaagaa acgacaaaaa
     9661 tgatagctaa taatgcaaga aacaaggagt ttaaatactt tccgacatat ttgaccaaac
     9721 gtgttggatg tacagtaagg aaataagcaa gtaaaaagaa aagagcagag aataaaaaca
     9781 tacctaatga atcatatttt tcaggcaaaa agggtttagc agccatttca aaagcagttg
     9841 ctgctgtccg cggtgtacca aagaatggtc cgatcgttaa atgaacaagg atcaagaaca
     9901 aggcagcata ccattttccg acaggtttag ctaagtcata cagaccatca ctttttgtca
     9961 ctactacagc taagatagct aataatggga aaagagcgcc tgagatggca aatcctacac
    10021 cagcgctgat ccagtttgat ccagccattt gacctaagtg gacaggaaag atcagattac
    10081 ctgacccgaa gaacatcccg aagataagtg aactgaccat caataattgc ttccatgaat
    10141 aagtttttgt ttcttccata aatttttcct ccaataatta tcattttgag taacaaaata
    10201 aacgttgcag aaactagaaa tgccactaat atcagattcc atgaaaacca ccaccttaaa
    10261 atataaaaaa aagccctttt ccacttggga aaagggctct agattctaga acggtaccac
    10321 cttttttcgc ttaaaaaagc ttcttcattc acggacgctt ttcgataaag ttatccgcta
    10381 gcctgataat ggaggcagcc acaatgatct acgttttcga cattgactcg gaaatgatgt
    10441 tcgtctattc gatttattgt catttctcac ttacatgact cactgggaat aaatcgctga
    10501 atagcctact gttttcctcg acgctttgct gtatatttat ttgttgacgt tataatacac
    10561 ctgttttttt tgaaatgcaa gaatatttca aatattttgt tttctttttt tgaaacaaga
    10621 aaaataccca gagaatactt gctaagttag acggaatact tgtcctctat cttatgcata
    10681 gcttacatga tcagcttttt ttattcaaac gagaccttta gagaatggaa gcagctattt
    10741 tttaacgttt cttggtactt atggttaagc aactctttta gaaccttttc ttatttgcta
    10801 tatagtttct tctgagtatg gtaaaatagg aaagaatgaa tcgaaaaaaa ggggatgtct
    10861 atttttgacg gaagagtcta gttctttaga gattagattg acggctgctg atgatgtcag
    10921 catgctttta ggtgcacacg acaagcatat aaaactgatt gaagaaacaa cagaaacaac
    10981 catccataca cgtggagaaa tcattcagat cattggtgaa caatcccaga tttccttggc
    11041 tgcctcagtc attcggacat tgcaggaatt gatcaaacga ggaatccatg taagtacacc
    11101 agatgtcgtg acggcactga aaatggggca aaaaggcaca ttagattatt ttgttgagat
    11161 gtatgaggaa gagatcgtca aagaccgtaa tggaaaacct atacgggtaa aaaacagcgg
    11221 ccaaaagaaa tatgtcgaat cggtagccaa acatgatatc gtatttggcg taggaccagc
    11281 cgggacagga aaaacttttc ttgcagtagt attagcaatc gctgccttga aaaaaggtga
    11341 agtgcaaaaa atcattttga cgcggcctgc tgtggaagca ggggaaaatc taggctttct
    11401 tcccggggat ctaaaagaaa aagtagatcc ttatctgcgt cctgtttatg atgctttata
    11461 ccaaattttt ggattagatc atacgaaccg attgatggaa cgaggcgtga tcgaaattgc
    11521 tcctttagct tacatgcggg ggcggacatt ggatgatgca ttcgttattt tggatgaagc
    11581 acagaacacg acagttgctc agatgaaaat gttcctgaca cgtcttggtt atcaatcaaa
    11641 aatgattgtc aatggagata cgagtcagat tgatctgcct cgagggacaa caagtggtct
    11701 tgttcatgca gaacgaacat taaagcaaat caagaaaatc gattttgtta attttgaagc
    11761 aagtgacgtt gttcggcacc ctgtagttgc cgaaatcatc aaagcttatg aaaaggcaga
    11821 tctccatcaa gaataagctt tgcttaggag gataagcaca tgttgaataa aacactgcag
    11881 aaactacaag aaaaacttgg aaaggcagca ttgcctttcc tgctgttctt gttttttatc
    11941 attatctgcg gcgttacatt tagtagtgtc aaacaaaagg ccaatgattt cagagaaggc
    12001 caagtagcag aagaaagtat ccgtgcaaac aaaacgatcg agaacacgga agaaaccgaa
    12061 caaaaaagaa aactggcagc agaagcagtc actccagaat acacttacca aaaagatctt
    12121 gcagatgacc agaataatcg gatcaagcaa ctatttgaat tgatcgataa gacgaatgat
    12181 gccatcaaca aatcttatga cgaaaagatt gataaggcaa aggaagataa aaatatccca
    12241 aagcctacta cagaagagcg gatcgccgcg ttgaagaaaa attttgaaaa tgtgaatgca
    12301 gaaaatgttg cgttttatca aaaactaccg aacaattttt attcgacgat atttgaaatg
    12361 actgaaactg agatccatac tgtgagggat gagagtctga agctcattga cgaacagatg
    12421 agcaaacaag ttcgtgaaag tgaattggaa gcttttaaac aagaagcaga agagcaaatc
    12481 caatatttga atgtcacacc agcacaacaa caaatgattc gctacttagt ggatcaaggt
    12541 atcgtagtga atgacgtatt gaatgagaaa aagacagaag aattgaaaca atccgcacga
    12601 gaagctgtac aaccagtgat gatcttccaa ggagaaatca ttgttcgtga aggtaatcaa
    12661 atcgatgctt cagctatgaa aaaattggaa ttactaggac tgactaatca aacaacttcg
    12721 atttttccgc taattgccat ggttttagct gttttgcttc aaatcggcgt actgatttat
    12781 aattcacttc aattccaaaa tgcaggagaa agaacaaaat acgtgctttt ctatgtgaca
    12841 gctatgtcag tcagcatagt gctgatgaaa tttttccaat tattccaaac ggaacaagct
    12901 acttatatcc cgctgtttta tcccgctgct tttgcaccac ttgttttaag ctttttcttg
    12961 aatcgaagag cagggatcat ggctggtatt ttccaagcag tatctgcact tttcattttt
    13021 tatgattcga ttggcacgaa ctttctaacg attatcttga tgtcttatgt tttctctggg
    13081 ctcatggcaa cgattgtcag acgtaaacgg atctcagaac aaggattatc tgctgccatg
    13141 tgggtcatta tttttcctgt gatgatggat gtcattttga tcgtctatca aggaatgagc
    13201 tttggtgatg caacgacttg gttaatgctc gtctgcgggt tcactggtgc actcttctca
    13261 ttcttgctga cgatcggtct acacccatat attgaattga tggtcaatga tgacagtgta
    13321 attgtattaa atgaactcag caatccaaat catcctttat tgaaacagtt gctggaagaa
    13381 gcgccaggta cttatcatca tagtatgatg gtagctaatc taagtgctaa cgctgtagca
    13441 gaaatcggcg gacgttcttt gctaactcga gtagcttgct attatcatga tatcggcaag
    13501 atcaaacatg ctagcttttt cgttgagaac ttgccttcag gagcggaaaa cccgcacaat
    13561 ttcttattgc cagaagacag taaacatatt atctttggac atgtgacaga tggagccaaa
    13621 atattggaag attataatat gccacaaatg gtgatcgata tttgcagaca gcatcatgga
    13681 accacgctaa tgcgttattt ctacatcaaa gcgaaagaac gcaatccgga agtaacagaa
    13741 gagcaattcc gttaccctgg accaaaaccg caaagtcgtg aagcagggat cgtcaatatt
    13801 gctgacagtt gtgaggctgc agtacgggca atggatcatc cgacgagcga aaaaatcgaa
    13861 agttttgtcc ataatcttat cgaagagcgt atttccgatg gtcaattaga cgatagtgga
    13921 ctgaccttga aagaaatccg aaaagtggaa aaatcattga tcagcggttt aagtagcacc
    13981 ttccattctc ggatcaaata tcctaaaatg aaaacagaag ttgagaagat aaaggaagaa
    14041 caagaagaat agaaagaagc gatgtataat ggatattacc tttatggatg aaacaaacca
    14101 agtctcagaa gagaaaatga aagaaatcga tgacttgctg caatttgcag catcttattt
    14161 aaagctgcca gaggaaacag aaatgtctgt tactttcatg gacaatgcgg caatccaagt
    14221 aatcaatcgt gattaccgtg gaaaagatgc accgacagac gtcatcagtt ttgctctgga
    14281 agaagaaggc gaagacgaaa tcccagtgat ttttgaagat ggagagaatc cattgcctcg
    14341 tgtactagga gacatcatga tctctattga acgtgctgct gaacaagcag aagcttacgg
    14401 ccattcatat gatcgggaac ttggcttttt agcggtacat ggattcttgc atatcaacgg
    14461 gtatgaccat atgacaccag aagatgagaa agaaatgttt ggtttacaga aagagatctt
    14521 ggatgcctat ggactcaaaa gataaaaacg tagagaaaaa caaacatttc ctgatgtctg
    14581 tggaatttgc tgtccaagga gtcaaaaccg tttttgagga agaacgcaat atgaggaaac
    14641 atgttgcgtt cggtatcctt gctttattta tgggcgtgat tttccagctt gatcggatag
    14701 agtggttatg gctgctttta gctgtttttc tcgtttggat agtagagatc atcaatactg
    14761 tttttgaaaa tgtagtggac atgtttacgg attttcattt tcatccgatt gggaaaaaaa
    14821 tcaaagatat ggcagctgga gcagttttag tgaccgcttg ttttgctgtc atcattgggt
    14881 tgattttatt tgtccctaaa atatatcatt tgttgtttaa ttaaaaagga gaacgtatat
    14941 gccagaacat aaatcagggt ttgtcgcaat cgtcggccgc ccgaatgtag gaaaatccac
    15001 attattgaac cgaatcgtgg gtcaaaaaat tgccattatg agtgacaagg cacagacaac
    15061 tagaaataaa atccaaggag tatatactac accagaagct cagctgattt ttatcgatac
    15121 accaggaatc cataaaccaa agcatcgtct aggagatttt atggtagaag cagcttacag
    15181 tgcgttgaaa gaagtggatg cagtcttatt tatgatcagt gctgatcaaa aacgcggtcg
    15241 gggagacgat tttattattg aacgtctaaa aaacgtgcat tctccagttt atctgattat
    15301 caataaaatc gataagatcc atccagacga gttgctggga atcatcgaag attattcgac
    15361 gcagatgcct tttacacaag tgattccgat ctctgctaca gaaggaaaca atgtcgaacg
    15421 cttaatggat gtattagtca gtgaaatgcc agagggccca caatattttc cagatgatca
    15481 agtgactgat catcctgaat acttcatcgt ttctgaattg atccgtgaga aagtcttgtt
    15541 tttaacacga gacgaagtgc ctcattctgt cgcagtcgtt gttgattcga tgaagcgaaa
    15601 cgagaatgat aaaatccata tccaggccac gatcatcgtc gaaagagata gccaaaaagg
    15661 aatcattatc ggcaaaggcg gaaaaatgct gaagcaagta ggaacgaaag caagaaagga
    15721 tatcgaaaat cttttaggag acaaagtatt tctagaactt tgggtcaaag tccaaaaaga
    15781 ctggcgagac aaaaaagtct atctgcaaga cttcggctac cgaaaagatg aatactgata
    15841 ttctgaactt cttataggtt aagtatagaa ataaactgga taaaaatccg cacgttgaga
    15901 aaaacggcgg aagatcgaaa cataaataat ctggatcggc agaatctgct tttccagatt
    15961 tattttttag gagggagaat atgagccagc ttgcagaatc gaaaggcttg attttatcta
    16021 gccgtaatta caaagaaaaa gataaattag tcaaaatctt tacggaatct tctggcaaaa
    16081 tgatgtttta tatcaaagga atccatcggc aaaatcagcc attagctccg gctttgctcc
    16141 cctttaccga agcagtctat atcggtaatt ttcgaagtga aggtttgtcg tttttaagca
    16201 gtgtcaaaga cgtccatgcg tttcggaaca tccaagaaga tatctttttg agcggttatg
    16261 gtacctattt gctaaatcta gtagatgcag caatcgaaga tcatcaatat gacccgcatt
    16321 tattccaatt tacgcaacaa gctctgcaac ggatggatca aggagatgat gcagaaatca
    16381 tcacgaatat ctttgaagtt caaatactgc aacgcttcgg aatcgcgcca atttggacgc
    16441 actgtgtcgt atgcggggaa acaaaaggaa aatttgacta ttcttccaaa tatggcggtg
    16501 tgatatgcga gaaacactgg ccaatggacg aacaccgtta tcatgcagat cctcgcgctg
    16561 tttatttcgt tcgaatgttt tcggctattt cttatgataa gatcagtgga atccacttga
    16621 aagaagaaac gaagcaggca atccgtgacc tcattgacca gttgtatgat gaatatgtcg
    16681 gtattcatct aaaaagcaaa aagttcattg atcaaatgaa gtcttgggca cacgtattaa
    16741 agacgccaga gaaaaacatg cctgaaaaag agatacctga aaaagaagaa taaatgaaag
    16801 atacaacaaa aaaatcaaaa ctatttgaca aatacaggga cttccgtcta ttattaaccg
    16861 tagattaaat agattgttca ctgagaggat ctattataca aacaagaaaa ctgcaaaatg
    16921 aatacgtaca ttgaccaaga tcagtaaaaa acacgtggtt tagcgagttc gggagagtgg
    16981 aagccggacc caaacgattt ttgaagaggc acttgcgagt acgataaatg aagctgccga
    17041 atctttattc ggaagtaggg tggaaccgcg aaattattcg tccctatgtc tgtaatcaga
    17101 catagggact ttttgtcgtt ttctttcctg tattcttatt gacagcaacg cttaaaaatt
    17161 gaaaggggca aaacagatga gtaaaaaatt gaccgtccaa gatatgattt taacattaca
    17221 aaaattttgg tcatcaaacg gatgcatgct gatgcaagct tatgatacag aaaaaggagc
    17281 aggtacgatg agcccatata cattcttacg ggcaatcgga ccggaaccat ggaatgctgc
    17341 ttatgtagag ccatcccgcc gaccagctga cgggcgttat ggagaaaacc caaaccgttt
    17401 gtatcagcat catcaatttc aagtcgtaat gaaaccttca cctgaaaata tccaagagct
    17461 ttatttagaa agcttgcgtc ttttaggaat tgatccatta gaacacgata ttcgttttgt
    17521 tgaagacaac tgggagaacc catcgatggg gtgtgccggt gtgggttggg aagtatggct
    17581 tgatgggatg gagatcacac aattcacgta cttccagcaa gtcggaggac taccttgtaa
    17641 accagttact tctgagatca cttatggact agaacgtctt gcttcatata ttcaagaagt
    17701 tgaaagtgtc tatgatctgg aatgggctga tggcgtgaaa tatggcgaga tcttcaaaca
    17761 accagaatat gaacattcca aatattcttt tgaagtaagc gaccaagact tgctactaag
    17821 taattttgac cgttttgaaa aagaagctaa acgctgtatc gatgaaaatc ttgttcatcc
    17881 agcgtatgat tatattttga aatgcagcca tacattcaac ttattagatg cccgaggtgc
    17941 agtatccgta acagaacgag caggttatct ttcccgtatc cgcaacatgg ctcgtgctgt
    18001 agccaaaatc tttgtggcag aacgcgaaaa attaggattc cctttattga ataaaaacac
    18061 agcaaatcca gaaaacatga aggaggctga ataagatggc aaaagactta ttacttgaaa
    18121 tcgggctgga agaaatgcct gctcatgttg tcacaccaag catgaaacaa ttagaagaaa
    18181 aaacagcaaa atttttagat gaaaaccaat tgacttatga tacgatcgaa acattttcca
    18241 caccgcgccg tttagcaatc aaagtaacaa atattccaga acgacaagcg gacagcgaag
    18301 aagaagtcaa aggaccagcc aaaaaaattg ctttggacgc agatggaaac tggacgaaag
    18361 cagctgaagg atttgtccga ggacaaggtc tgacgacaga tgctatcact tttagagaat
    18421 taaaaggcgt ggaatatgtc tatgtaacga aatacactca cggcaaagaa gcaaaagaag
    18481 tattgacagg tttagcagat gtcattaaaa gcctcacttt cccagtaacg atgcattggg
    18541 cagaatacga ttttgaatat atccgcccta ttcactggat cgtggcattg ttagatgacg
    18601 aagtaatccc attcgaaatc ttggatgtca aaacaggcaa agcctctcga ggtcatcgtt
    18661 tcttaggcga agatgtaaca tttacgcatg caaaagaata cgaagaaaaa ttaaatgaac
    18721 aatttgttat tgcttcaccg caaaaaagaa aagaaatgat catcacacaa gcaaacgaat
    18781 tagcagaaaa aaatcactgg atcattgatc tagatgaaga tttactagaa gaagtcaaca
    18841 atctagtcga atatccaact gtcttttctg gcgacttcga agaaaaatat ctatctgttc
    18901 ctgaagaagt actagtcact tctatgaaag aacatcaacg ttatttcgaa gtacggaacg
    18961 atcaaggtat gttgcttcct cattttattt ctgctcgtaa tggcgatcgg gtaaaattgg
    19021 aaaatgtggt acgaggcaat caaaaagtat tgactgctcg tttagaagat gcggaattct
    19081 tttataatga agacaagaaa gcttcaatcg accaatacgt tgaaaaatta aaaactgtta
    19141 ctttccatga aaaaatcggt agcatctatg agaaaatgca acgggtacaa gtcatcgctc
    19201 gcatcatagg cgaacaagta ggactatctg aggaagaatt gatggatctg caacgcgctg
    19261 ctgaaatcta taaatttgat ctagtaacca acatggtagg agaattccct gaactacaag
    19321 ggatcatggg agaaaaatat gctctgctta aaggcgagaa accagccgta gcaacagcga
    19381 tccgtgaaca ttacatgcct atatccagtg aaggagatct acctgagact acagtaggtt
    19441 ctgttttagc gattgctgat aaattagata gtatcttttc tttctttgca gttgggatga
    19501 tcccaagtgg ctcaaacgat ccatatgctt tgcgccgtca agcatatggt gtcgttcgaa
    19561 tcatcgaatc gaaaaaatgg gcctttcctt tgtctgttct gcaagaaacg attagtgaag
    19621 ttatcagtaa agatacggat cgttttggca ttggcttgtc agcgggacaa caacaggtga
    19681 tcgactttat caaaggccgt ctgcgccaat tattgacaac taagaatatt cgtcatgatg
    19741 tcatcgaagc tgttttgaac gcagaacaaa aagatctgac aaaagtattt gcagcagcac
    19801 aattgttcaa gcagcatctt gcagatgaag acttcaaacc atcgatggaa gctttgacac
    19861 gtgtcgtgaa cttagcgaaa aaagcggagc ttgaaaaaca atcagaagta gaccctgaac
    19921 tgttcgaaaa cgaagcggag aaagaactgc acaaagcagt agaggcaatg aaaacaagct
    19981 ttgctgacca aacgatcaac gaaaattatg atcagcttgt aagccttcgc ccactgatcg
    20041 aaaattattt tgatcagacg atggtcatgg cagaagaacc agcagtacgt aagaaccgat
    20101 taacgcaatt aagtcaaatt gctgccatgg ctttgtcact agcaagttta gatcaaatca
    20161 tcacaaaata atttctaata tgtaaataaa ccagagaaca ggcacaaaat aggctatttc
    20221 tctggtttat ttgttataaa tataaataac tattattaat tcaatgttaa ttttttttgt
    20281 tattttattt tttgtgtaac ttttaaaaac acctatgaaa attaaggaaa taa
//
LOCUS       DAZUMO010000048        20182 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000048 DAZUMO010000000
VERSION     DAZUMO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.048"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..900
                     /locus_tag="ACG8ER_001839"
     CDS             10..900
                     /locus_tag="ACG8ER_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS5050415.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(973..1797)
                     /gene="nadE"
                     /locus_tag="ACG8ER_001840"
     CDS             complement(973..1797)
                     /gene="nadE"
                     /locus_tag="ACG8ER_001840"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS5050416.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1809..3281)
                     /locus_tag="ACG8ER_001841"
     CDS             complement(1809..3281)
                     /locus_tag="ACG8ER_001841"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS5050417.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3918..4280)
                     /locus_tag="ACG8ER_001842"
     CDS             complement(3918..4280)
                     /locus_tag="ACG8ER_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5050418.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4500..5252
                     /locus_tag="ACG8ER_001843"
     CDS             4500..5252
                     /locus_tag="ACG8ER_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5050419.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5392..6315)
                     /locus_tag="ACG8ER_001844"
     CDS             complement(5392..6315)
                     /locus_tag="ACG8ER_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5050420.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6330..6602)
                     /locus_tag="ACG8ER_001845"
     CDS             complement(6330..6602)
                     /locus_tag="ACG8ER_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050421.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(7072..8697)
                     /gene="groL"
                     /locus_tag="ACG8ER_001846"
     CDS             complement(7072..8697)
                     /gene="groL"
                     /locus_tag="ACG8ER_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS5050422.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(8748..9032)
                     /gene="groES"
                     /locus_tag="ACG8ER_001847"
     CDS             complement(8748..9032)
                     /gene="groES"
                     /locus_tag="ACG8ER_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS5050423.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            9257..9919
                     /locus_tag="ACG8ER_001848"
     CDS             9257..9919
                     /locus_tag="ACG8ER_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5050424.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(9995..11287)
                     /locus_tag="ACG8ER_001849"
     CDS             complement(9995..11287)
                     /locus_tag="ACG8ER_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5050425.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            11457..12086
                     /locus_tag="ACG8ER_001850"
     CDS             11457..12086
                     /locus_tag="ACG8ER_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5050426.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(12189..12998)
                     /locus_tag="ACG8ER_001851"
     CDS             complement(12189..12998)
                     /locus_tag="ACG8ER_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5050427.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13053..13922)
                     /locus_tag="ACG8ER_001852"
     CDS             complement(13053..13922)
                     /locus_tag="ACG8ER_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS5050428.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(13923..15239)
                     /gene="yycH"
                     /locus_tag="ACG8ER_001853"
     CDS             complement(13923..15239)
                     /gene="yycH"
                     /locus_tag="ACG8ER_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS5050429.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(15236..17071)
                     /gene="walK"
                     /locus_tag="ACG8ER_001854"
     CDS             complement(15236..17071)
                     /gene="walK"
                     /locus_tag="ACG8ER_001854"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS5050430.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(17076..17780)
                     /gene="yycF"
                     /locus_tag="ACG8ER_001855"
     CDS             complement(17076..17780)
                     /gene="yycF"
                     /locus_tag="ACG8ER_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS5050431.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(17970..18830)
                     /locus_tag="ACG8ER_001856"
     CDS             complement(17970..18830)
                     /locus_tag="ACG8ER_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5050432.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(18817..19386)
                     /locus_tag="ACG8ER_001857"
     CDS             complement(18817..19386)
                     /locus_tag="ACG8ER_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS5050433.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            19767..19839
                     /locus_tag="ACG8ER_001858"
     tRNA            19767..19839
                     /locus_tag="ACG8ER_001858"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19800..19802,aa:Lys,seq:ctt)
BASE COUNT         5849 a         4220 c         3456 g         6657 t
ORIGIN      
        1 gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga agcattagct
       61 agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa tgtcaaagat
      121 ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa aaagctagag
      181 gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc
      241 gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac gaaagaaaca
      301 tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg tttagctacg
      361 atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc tcgaaggatg
      421 gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc taatttttta
      481 aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc tgaatttcct
      541 ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt ggagctagga
      601 acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc tgattggtat
      661 ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa acacttgacg
      721 aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa agagcaagac
      781 acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg ctgtacttta
      841 tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta ctacagctga
      901 tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg gacggctgtt
      961 tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg
     1021 ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc
     1081 ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat
     1141 tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt
     1201 caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc
     1261 ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt
     1321 ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg
     1381 tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg
     1441 tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa
     1501 gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc
     1561 gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc
     1621 caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag
     1681 aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt
     1741 tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta
     1801 ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata
     1861 gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag
     1921 tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg
     1981 tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg
     2041 cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag
     2101 atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc
     2161 ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct
     2221 gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact
     2281 agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca
     2341 ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag
     2401 tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca
     2461 cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg
     2521 tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa
     2581 gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa
     2641 tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat
     2701 atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt
     2761 ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct
     2821 tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc
     2881 atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatc
     2941 aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat
     3001 tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa
     3061 gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct
     3121 gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca
     3181 tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc
     3241 gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg
     3301 caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat
     3361 tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct
     3421 aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa
     3481 tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag
     3541 attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg
     3601 tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg
     3661 cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg
     3721 aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt
     3781 aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat
     3841 caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt taccataaag
     3901 atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat cactacaata
     3961 tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt cactcctaca
     4021 tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata gtcgatttct
     4081 tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc atatgtgtct
     4141 accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat agccgtattc
     4201 ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt actattcaca
     4261 ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta gatccaatct
     4321 taagttatgt tacctttcct agcattaagg aatcggttac aattgaacct ttaaagacaa
     4381 aaggatttac cttttatcac ttaatagtat aacataattt catgagaaaa aaatacaatt
     4441 ttttcttgac catctcgtta cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa
     4501 tggagtacac aataaagaaa atgtcagaga tatcaggagt cagtccgaga accttgcgct
     4561 tttatgatga gattggactg ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt
     4621 atggcaaaaa agaagtcgat cgtctgcaac acatcttatt ttatcgaaca atggcattca
     4681 agttggatga tatccaagag gtcttagaca atccatcttt tgaccaccag aaagctttaa
     4741 tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat cgataccctc ttgacaactg
     4801 tccaacagac acttgatatg tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg
     4861 aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc ctatggacaa gagattcgac
     4921 aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa atggatgaat atggataaag
     4981 aaacgtatga agaaatgcaa actgttgaaa agcagatgtt atttgatctc actctttact
     5041 taaaagaacc agaagatgaa agcttagcag accgtatctt ccaagctcat aagaagtggt
     5101 tatcttacag ctggcctgaa tatcagccaa aagcgcataa aggactagga atgatgtacg
     5161 aagccgatga acggttcact gcatattatg acgagcgaag tggtcatggt gcagcaaaag
     5221 ctttaaatga aattatccaa aaaaaggcgt aatcttttac atgatccaac aaaaaagtac
     5281 acgtaaaatg acctaataac aaaacagcag acacccaagt tagattttta tgctaacttt
     5341 tggtgtctgc tccattcttt cacaagtttt ttcatcctac tttaaatata ttcatcgttg
     5401 aataaacgta taaggtattt tcactttatc ctttttttct gtaagaaaca attgaaatgc
     5461 catttctccg caagcttcca gatggtggtc gatggttggg aaaccaagaa gctcgctgat
     5521 cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct tgcagatagc tttgcaagat
     5581 gcctgctgca accatatccg aatttgtaaa gacaccatca actccaagct ctttaaaaaa
     5641 gaagcctgct tgttttcctt tctctgcatc tatacaatcc caaaaaacaa gtgattcatc
     5701 aaaagatgga aaaagttgat gacatagctg caaagtgatt tttgagttat aacttagcct
     5761 gccacttcgc cccaacgtga tgcccaattg ctttatacct tgctgcttca aataagtaag
     5821 accttctgta attgattgtt ctcgatcaat aaagacacag cttgcctgtt ttcccttgat
     5881 ttcttcacaa aaaactagag gtccatattt ttgatatgcc aataaatcat ctagtgtatt
     5941 ggcacgagac gtaatgatca atccatcgaa agctttagct gcaaattctt caagatacct
     6001 tcgctctttt tctttctgat agcccgtcgg taaaagtgtc actttgtaat cataatcaaa
     6061 cgctgcttca atgatcccac tgatcaactg atcaaaatac ggatgattgg tataaggcag
     6121 aatcactcca atgcttttcg tttttccata acttaactgt ctggcatttg cattagggat
     6181 ataatcgact tctttcatga ccgcaagtat ttctttcctt ttttcctcat ctacatatgg
     6241 atgtccattg attacacgtg aaatagtaga aactgaataa cctgtgagct ttgcgatatc
     6301 tcgaatgttt gccactgctg cttcgccacc tatacaaaaa gattttcttg taaatagtct
     6361 accacagctg gaccgcattc aaaaaactga tacatttttc cttcatgcga aaaactgatc
     6421 cgctgcccgc cccaatatat atcttttgtc aaattttcga tttcattcat tggtatttct
     6481 tttactatat tcctattcaa cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac
     6541 tcgacaaacg cataaaaata attgttcgtt gtgccatctg ttatctctac ataagtattc
     6601 attgcaagcc ctccttttga tactttgagt atagtgtatg aaacgcgtct catagcaagg
     6661 agtttgtttt gccaaagaaa agttttattc ctctttcatt attaccaaag tctcttattt
     6721 taaattgaat acattataat gatactgtaa atatatcttt attattagtg aatggatatt
     6781 tacttttaat agataaaata ttataactat taaaaataac tattttcatt ataattacac
     6841 acaaaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
     6901 aaacagcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa
     6961 agcaatttcc gtgaatttct tcttatttct tggagttaaa cgcttctgtt ccagcctctt
     7021 tttatctgtc tttcactaat gatcagttgt agataagcaa gttgcttttt cttacatcat
     7081 accgcccatc attgatggat ccattgcagg agctgctggc gctgctggtt ctggtttatc
     7141 tgcaacgact gcttctgtcg ttaataacaa ggcagaaacg gaagcggcat tttgcaaggc
     7201 agaacgtgtt actttagttg ggtctacgat acctgcttca accatgttta cccattctcc
     7261 agtagcagca ttgaaaccag tgcctagttc cacgttcttc aatttatcaa caatcactga
     7321 tccttcataa ccagcgtttt cagcgatttg acgaattggt tcttctaaag cacgaacaac
     7381 gatcttgata cctgttgcca cgtctccttc tgcttctaca gcagaaactt tgctgataac
     7441 gttcactaga gctgtaccac cacctgagac cattccttct tctactgcgg cacgtgtagc
     7501 attcaatgca tcttcaatgc gtaattttaa ttctttcaat tctgtttcag tagctgcacc
     7561 aactttaaca acagctacac cacctgctaa tttagctaag cgttcttgta atttttcgcg
     7621 gtcaaaatca gaagttgttt cagcaatttg gtttttgatc aattgcacac gagcttcgat
     7681 tgcttctttt tctccagaac cttctacgat cgttgtattg tctttatcta caacaacttt
     7741 agaagcattt cctaagtttt cgattgttgc atctttcaat tctaaaccta gatcgtcagt
     7801 aattactgta ccacctgtca aaatagcgat atcttcaagc attgctttac gacgatcgcc
     7861 gaaaccagga gcttttacag ctactacgtt gaatgttcca cggattttgt tcaatacaag
     7921 agttggcaat gcttcaccat ctacatcgtc agcaataatc aacaatggac gtgattgttg
     7981 caagatttgt tctaacaatg gcagaatatc ttggatgtta gagatttttt tgtctgtgat
     8041 caagatatat gggttttcta gaacagcttc cattttgtcg ttatctgtta ccatatattg
     8101 tgataagtaa ccacgatcaa actgcatacc ttctacaaca tctagttctg tttcgatccc
     8161 ttttgattct tcgattgtga tgacaccgtc gtttccaact ttttccattg catcagcaat
     8221 caaatgtcca actttatcag aacccgaaga aacagctgct acttgtgcaa tggcttcttt
     8281 tgaatcaaca acagttgaga tattatgcaa ttcttcgact gctgcttttg ttgccaattc
     8341 gatcccgcga cgaatgccta gaggattagc acctgcagtt acgtttttta aaccttcgcg
     8401 aacgattgct tgtgtcagaa cagttgctgt cgttgtaccg tctcctgcaa tatcatttgt
     8461 ttttgatgct acttctgata caagttttgc acccatgttt tcaaaatgat cttctaattc
     8521 gatttctttt gcgatcgtca caccatcatt tgtgatcaat ggagagccgt aagatttttc
     8581 tagtacgaca tttcggcctt taggtcctaa tgttactttc actgtatctg ctaatttatc
     8641 taccccgcgt aacatagctg cgcgtgcgtc ttctgcaaat ttcaattctt ttgccatgat
     8701 tgattcacct cattgattat ttttgattat ttttttatct tccaaattta ctcaacaatc
     8761 gccatgatat cttttcctgc gacgatcaga tattcttttc cttcgtattt tacttctgta
     8821 ccggcgtatt tttcgaacat aacttggtcg cctgctttta cagcggcagg aactttttca
     8881 ccgttttcta acaaacggcc ttcaccgact gcaacaactg tcccagtttg gggtttctct
     8941 ttagcagcag aagctaaaac gattcctccg acagtttttt cttcttcttt tgcgacttcg
     9001 ataatgacgc gatcacctaa tggttttaac acgataaatc cctccgataa ctcttttttt
     9061 aatctgttag cactcttaac cttcgagtgc taacctacat ttttaataat actcattctc
     9121 cctctttttt gcaagctttt aaatcaaaga aaagaaattt tattctgaca gctgcttatt
     9181 gaaattatga tataattgaa atcaatcgat ataagcttgc cttgcatgct tgttatccct
     9241 taaaaggagg aattacatgt ctttaaaaaa atatagcttc ctcagcattt tttgctatgg
     9301 tctaattttt atctcaccct tgatcctagc ttcgatagga cttgtaaaat ctacgtctga
     9361 attgatcaca gcaactgcag tcatgtatat actaggcgcc gttgtcctag ctatttttta
     9421 ttttaagcaa cgtgaaccct tagcgatcga atcagcggta aaaaaagctt ctgctccaaa
     9481 gattgttatt tatggattgg ttggaatctt tgtcgcattg attctgcaaa gcatcgctgt
     9541 catgttagaa tctgtattgt ttggcaaagc aaccccttcc gagaatacac aaaatatcat
     9601 ccagatgatc atggaagcac ccgcttttat tttggcgaca actgttgctg gacccatcat
     9661 ggaagagttc gtattccgcc gcagtatact ggggatcatc agtcgatatt caaatttttg
     9721 ggtcggagca gtgatcagct ctttgctgtt tgcgtttgct cataatgatg gtcatctgct
     9781 aatctacttt ttcctaggtt tcttcttcag cctagagtac aaagcaactg gtcggatctg
     9841 gacatcgatg atcacacacg taggaatgaa cacattagtt gttcttgttc aattagctgt
     9901 ccaaaaaggg ctagtataaa catgactata aatactcctg tcatcctaga ttggtgattt
     9961 tcccaatcta aagcgacagg agtatttttg ttttttagtc gttcatcttt tctgctgctc
    10021 catcattttt tgtctgtctt tcctttttgc agtttgcaga atctattttt ttcaaaaatg
    10081 ggaaataaat gaaaaaacta attgacaaca cgataccttg taaaatcgct gcaggcactc
    10141 cgcctactaa gaaacccgag aggatcggcg gtgttgtcca agggacttgg actgctgtaa
    10201 ataaaggaac gaatccaaaa tataaagcgg aataggtaat caatcctgaa acgaccggtg
    10261 cgaaaataaa aggaacagcc atcatcgggt tcatcacgat cggtgtggaa aaaataattg
    10321 attcattgat gttgaaaaag gctggtaaaa gagcaagatt tcctaattct ctgtatttct
    10381 tcgatttcgc aaaaaccgtc ataaaaaata cagcccctaa cgtcaatcca gcacccgtaa
    10441 ccgtcatgaa ctgatcgaga aattgctgag taacgatatg tccgccattt gccaccgtca
    10501 actcttgtcc tgaggctaag atttctgcat ttgctaatga attcgcttgc aagacgggac
    10561 taaccaaccc attgaccatc gcagatccat gaagaccaaa taaccataaa aacggaacaa
    10621 aaaaacctaa taaaagcaca ccaaacaacg tgtctatgaa cgattgaatg ggaagctgga
    10681 catatgtcca tactccttga acaacagtca ttctgaacaa atgttgaaaa accgctgaac
    10741 cgataagcgt aaaagtgact ataacaatga ttggaatcaa tgaagtaaaa gactgccaaa
    10801 cattttcagg tatcttctct ctttgcttcc accctttcgc aagtcttact aaacgtgcat
    10861 agccccatcc gatgatgagc ccagttgtaa ctgctcctag tatcccttca gcacctgtcc
    10921 agacagtatc tatctgagta tttagtggct gaataagcag tagataagca gacaaggctg
    10981 ttatgccgac tggtaagcga tcgatgtctt cttcttttgc ataagcgtat gcaatggcaa
    11041 aaacgatcca aaaggctatc acatcaaatg tagcctggta tacttgtgta aaatacatag
    11101 aaaatcctgt tttgtctatc cactgagcga tgtaatccac aggaaaatga gcaaataaga
    11161 aaaacagcga gccaacaata attattggga tcgtgtaagt cattccatta cgcaaagcag
    11221 ttaagggtct aagattcaca acagatgtta tcattggtaa aaaggcacga tctaaccact
    11281 ctttcatttc ttatctcctt caacttctat ttttatcaat cagttttctt aatttaacta
    11341 tacccaatga tcacgaacca tgaaaggaat gcgcactgag cttttcaaaa atcggattct
    11401 ttgacgaata ttcagcaact gcttacaatg gacaggaata aaaaaggagg ttttttatga
    11461 aaattgcact cattggagct actggccatg caggttcatt tattttagac gaagcattgt
    11521 ccagagattt agacgtgacg gcaatcgtgc gtcatccaga aaaattgcca tcagatgttc
    11581 cgtttataca aaaggattta tttgagttga caactactga tcttgcatct tttgatgttg
    11641 tcgttgatgc tttcaacgct cccaaaggaa aagaagaaat gcatcaaact tctttgtccc
    11701 atttgattga gatgcttgaa ggaaccgata cacgcttaat tgtcgttggg ggtgcctctt
    11761 cgttattctt agatacagaa aagacgactc gtatgattga ccaagtacct aaaaatgcac
    11821 ccttctatcc aacagctttc aatatgtacg aaggattgtt aaaattgaaa caatctaaaa
    11881 atctacattg gacttatatc agtccagctt ctttatttga tccacatggc aaacgtacag
    11941 gtacttattt gttaagtgat gattatttac aaagaaacgc tgctagcgac agtacgatca
    12001 gcatggcaga ttacgcgatt gcattagtag atgagatact ggatgagaaa catgaaaagc
    12061 agcacatcag tgtagtcagt cgataatacg gttatttttt taatatgcta aaaaaataaa
    12121 aatagaagat aaaaccaacc ctgattgatt ttatcttctg ctttttatat ttttattgat
    12181 tgaactgttt aaatcgaaaa tagctcagtt gcttcatctg gatctgtatc atagatttca
    12241 aaatcatgtc caacgccaaa atctttttct ttcaaaacat tttccattgt taaatgagca
    12301 agttctttca tattgttttc cttgctaaga tggccaagat agattcgttt ggtccgatta
    12361 ccgatcacat cagtcatgac aagtgcaccg tcttcgttgg aaagatgtcc tctatctcct
    12421 aaaatacgct gttttaaatt ccacggataa ggtcccatgc gtaacatctc aagatcatga
    12481 ttactttcca tcaaataacc atcggcattt tcaatgacac ctcgcatata atcactgcaa
    12541 tatccggtgt ccgtcaacat gacgaatgac cgattttctt tatggaaacg ataaaattgc
    12601 ggagccgctg cgtcatggga gacgccgaaa ctctcaatat cgatatcgcc gaatgttctg
    12661 actttcccca tctcaaatag atgtttttgt tccactggta catttccaat cagtggttcc
    12721 atcgcttccc aagtctgctg attggcaaaa acatccaaat gatacttacg agccaagaca
    12781 cccacgccat gtatatgatc tcgatgttca tgcgtcacaa gtattccatc taattggtcc
    12841 ggtgtccgat caatttgagc catcaaggaa gtgatttttt taccgctaag tcctgcatcg
    12901 accaaaagcc gttttttttc actttcgata taaagtgaat tacccgaact tccacttgct
    12961 aggatactga ttttaaaagc tggtttttct tcagccattt gactcctact tcccttctta
    13021 ctttgtaaac ttttttcatt atactcgctt ttttattgat cttccacttt tggtacagta
    13081 ttgtttgtca cgatcgtatt gctgaaagcg ttgacgctct caatttggac cactttatcg
    13141 cttgtttcga tcgctacatg ccaaacagga acatacacat ttttttcccg gactttcaga
    13201 atacaggaat aagctagctg acgccataaa atttttgatc ctcttggtat tttgttattg
    13261 atgtacaatg tattgattgc ttcctcttcc gtatacagtt ccgtcttttc gcgcagctgc
    13321 tcgatatcag ataaatgcgt ctgcatgtat ttcgtgatgc tataatcatt atcgtttttt
    13381 tctaaagaaa gaacgatttt tgctgtatca tcattgaatg ggatgccttc atactcttgg
    13441 cttgcttgga tttctagtgg ttcttcttct aaatgagaaa agtttttcgt gtacacataa
    13501 tcattaccga ataaaatatt gtctttgtta gataaaaaat cttccaagga actactaatt
    13561 ttatctggat cgatcacgtt cttttcagca tctgaactgg ataaagattt cgtcaaaaca
    13621 tcaccgtcta aaaacacacc acttgccagc aagccgcttt taccagcctt gcgataattc
    13681 gctaatgaag tgctaagatt ggtctgctcc gcgctaagat aatagccttg cttgttctca
    13741 gacgaaatct tccccgtgta agtgatatcg tcgctagcca gacgttgttg gattgattca
    13801 gtctgattag agcgaatgac attgttttct tggtataccg cgtcatgata actgctgaat
    13861 aaaaacacat tcaaccctaa aaaagcgacg aaaaatatcc attcgatttt tttgaaatcc
    13921 aattaattcg cctccaatcc aggtaatttt tccagaaggt cgttagctga ataccagtta
    13981 tctttatatt tcacatacca ttcaggtaag agatcaacga cacgattcgt ttctttgaca
    14041 ttttgccatg tatagccaac gatcactgaa ccaatcttgt tgctatctgc tcccttagct
    14101 tttaactgct ggatgatttc attcgtactt gcaagttcta cttcttcgtc tgaaggtatc
    14161 ggcacttgaa tcgtattcaa actggtttgg atattcactt gcacagaacc attcgtttca
    14221 ccgccaactt ccacaccaat ctgtcctctg ctgtctgtcc caaaaaccgg gaagccttcc
    14281 acgaatatcc gataatcgat cgtattgtca ctgcggtcaa aatatctgag gttccctaaa
    14341 gaactgccta atttgctaat ataggggaaa ctttcagaaa atacactagc attttctttt
    14401 ttctctggca gctctccacg aaaatcaact acttgcgttt ccgaatggat agacattgtt
    14461 tcattcccac cgtaaaaaat caattcattg ctttcatcat tatttttcac gctatctggt
    14521 ttctggaaaa aagcatttct aaaaagtgta tagggttgct gtgacaggat ataactatac
    14581 ttcttcagtt tgatcgaatg tttcgtagca tattgcgtgt cgatcgtcga tggttctgtt
    14641 gtcatttctg tccattttgt gtcttttgct gttaattctt tattgatatc tgtccaattc
    14701 aacgaaatat ttgcttcata tacgagatgg ttcgaataat tcaaaaatcg gattttttta
    14761 tttccttgat cgaattgtat tctagtaaaa tacatatttt cgctttcttt tgccaagttc
    14821 gacacatcta aatctaaatc aaatgcttcc ttgtattctc ttagcaaaaa aggcgcatta
    14881 taggccaact caaaaccaga gttcagattt ttcacctgtt caaactgtga tttgtctcct
    14941 gaaactactt cagaaagctt tccaaatctt gctttatcga tgatcaattg caatcggtac
    15001 acgagatttt cactgttggt ttcttggatc attcccgaat ggtgccatgt aacatgaagc
    15061 ggaagaaaga cttcggaagt ttttcgattg ctttgagact cgatcatttg agtgtttgtc
    15121 tcatctacgt tgattgaact ttttcctgct gggctcagcc agattgcata agagaagtat
    15181 atgcttaacg caaccatcaa aatgagtcct atacgaacga ctttttcaga gattctcatt
    15241 cccaccattc ctcctcataa ggttcatatg gcagcgagat ataaaaagtc gacccttttc
    15301 cttccttgct ctctgcccaa atagcgcctc gatgcgcctt aatcacttct ctagaaatag
    15361 ctaatccaag tcctgtccca ccttgtgctc ttgcacgtgc tttatctact cgatagaacc
    15421 gctcgaacac tcggttcaga tcttttttag gaatccccaa tccttgatcg gtgatactta
    15481 aaatcacgtt attatgcgtt tctaataagc gacaagtaat cgttccccca tctggtgaat
    15541 acttgatcgc gttattcatg atattatcga ctacttggat gattttatct gtatctactt
    15601 cgacccaaag atctctttgc gtaaattcac gacggatctt ataatttttt tcctgatttc
    15661 ccaccatcat atcaaaccgg tcaagaacaa aattcactaa ctcgttgaaa ttgacatatt
    15721 ctaattgaag ctgggtatta cctgtatcca tgcgagatag atttaagagg tcattgatca
    15781 ttcgaatcat tcggtctgtt tcatctagcg ttaccttcaa aaaatttggt gctacttcct
    15841 cgtctttcca cgcaccttcg ctcaatgctt cgatataact acgcatactt gtcaacggtg
    15901 tacgaagttc atgggaaaca ttagagacga attcacgacg ttcccgttct gttttttctt
    15961 gttctgtcac atcatgcaag acagctacta atccgctgat aaaacctgat tcgcgacgga
    16021 tcatagaaaa gtctactcgc aagatcatct gatcactttc caatgtactg gttgaacggt
    16081 caagtaccat ttcgtttgga tcttctaata atttccgcaa cgtatattct tcttcgatat
    16141 ctaaaagtgt caaaatcgat tgtccgattg cttcctcgtt tttgatatct agcaaggaaa
    16201 cagccatatc attgatcgta atgactttac ccctgcgatc tgtcgcaatg acgccgtctg
    16261 tcatatggga tagcacgcta tccaaacgat tgcgttcagc ttccattgta tcttgagctt
    16321 cttcaatacg ttcagctaac tgattgaagg tatcagccag ctgtccaagt tcatcttgtc
    16381 catgcacttt gactttccga ctgtagtctc ctctagcgat tcgaatggct tgttctctca
    16441 tttcaccgat cggctgtgta atcgaacgag caactaaaac agatacgatc attgaaataa
    16501 atgccgcaat tagtgaggca gtaaagaaaa tgactgctgt gttattgatt tccgaatatt
    16561 tttgttcgat attacttttg acgtaaagag ccccgataac ggcatcgccg gtcggggaca
    16621 ggatcggttg aacattgata ttcacccgtt tatcgtcgtt atctaaagca acataacgtt
    16681 tagtcgtgaa atcattgata tcaacataat catttttctt tcccacagcg ctttgttcat
    16741 tgacatcggt tgttcctcgg atgatccctt tttcgtcaac gacccgtatt tcgatgatat
    16801 cgctagtcgc actgttttca ataaatcttt tgatattcgc atttacctct tcatcatctg
    16861 cattatcttt tcgattcaat tccgggctga ttgttgttgc taatgattcc accgtttgat
    16921 tcatatcttt aataaatgta ttgatcgtgg aacgttctaa accacgtata aagtaggcac
    16981 caataatttc gatcgagatc aataaaatca gaataaaggt aatagcgatc ttaaaattga
    17041 ctgatcgaaa aaagcggact tttcttttca tccaattact cctgttctgg gttacgcaaa
    17101 tagtaaccga caccacgacg tgtcactaaa taatttggat ggcttggatt atcttcgatc
    17161 ttttcacgca aacgacgtac agttacatcc actgttcgta catctccaaa ataatcataa
    17221 ccccaaaccg tttgtaataa atgttctctt gtcatcactt gtccaaggtg cttcgccaag
    17281 taaaagagta gttcgaattc acgatgtgtt aactcgattg tttctcctcg tttcgtcacc
    17341 atgtaagctt ctggatggat cgtcaaatca ccaatcgtca attccgcagg tgctgcctct
    17401 tctggttcct tcgcagccgt ggcaccgcga cgaagatttg ctttgacacg tgcaactaat
    17461 tctcggttgg aaaatggctt ggtcacgtaa tcatcggcgc ctaactctaa gcctaggact
    17521 ttatcaattt cagaatcttt ggctgttacc ataatgatcg gcatatcata agttttgcgt
    17581 acctcacgag caacttctaa gccgtccatt ttaggtagca tcaaatcaag taaaatcaaa
    17641 tcaggttcta cctcagcaac tttttccaag gcttcttccc catcatatgc agtataaacg
    17701 tcatagcctt ctttggctaa attgaatttg acgatatccg aaatcggctt ctcatcatcg
    17761 acaactaaaa cttttttcat aaagagccac ctcttatata ttattgtcat accttctcat
    17821 tataccgtat ttctgctttt gtttcatctt tgcctataaa acaaaagaga aaattaaaat
    17881 gaaagtgcaa aatactttat tattttttgt tctaacttca ataaaaaagg aaaacactga
    17941 ttttcacctc agtgtttcct ttttttgatt tatccttcta aaacttgtac accgacaccc
    18001 aataatcccg gaccagtgtg gactactaat gccggagaaa tctggccaaa gtagatttgg
    18061 tttgcttgtg ggaactctct tttaagtctc gcttccattt caattgcttc ttctttcgca
    18121 tccccatggg caacagctaa gttgaagacc tttgcattac cgattttttc tttgacgtaa
    18181 gcaaccgttt tatccaaact ttttttacgt cccctagctt ttcctactgt gtaataaatc
    18241 ccttcttcat tacatgagat gattgggtta agctttaaag cggttcctac aatagaagca
    18301 actaaaccga tccgtccacc tttttgaaga tattccaatg tcgcaacatt aaagaaaact
    18361 tttgttcttt cgactaactc attcagatta ctgatcagtt gatcccattc tactccttct
    18421 tccagccatt ttgctgcttg gatcgcagaa aaacctgcac caataccaat atttttcgta
    18481 tctagtacat atgcttccaa ttcttcttgc tgttgtgcaa ttaaacgaac cacattatat
    18541 gtcccgctca atccactgga aattgttacc gcaagtacct tttcataacc gtcttttttg
    18601 atttgctcaa agatttctgt gatcgactca ccatccggca aagatgtacc agggatttcc
    18661 tctggcaaac gttgatagat ctcttcaggg gtaatgtcaa ttttatctgt atatacctga
    18721 tctttataaa taattttcaa tgggatcata tacatctcgt atttttttat caattcttct
    18781 ggaacatccg tacctgaatc tactaaaatt gcgattttat tgtttttcat ttgttttctc
    18841 ctcttttaaa tattcggttt ctagaacaat cgttttttcc gcaagaagtt tcattgcaaa
    18901 cgataaagtt gccgctctta cagcaatata ttctacaggg gttgcctggt cgaatttttg
    18961 cggatgagat ttaccttgtg ctaattggct gaccatccac acagctgctt cttgttcatc
    19021 gcaaaataga ttataggctt cacgaattcc tactgttttt ccttggaaca atatcccttc
    19081 tttgatttca ggaatagtca atacttgttt caacgtagtg attgcaagta aaaatgctac
    19141 gtgttctttg ttatatcttt ttttaactgg tgctggtatc aaacctagtt taacgtaatt
    19201 gtttaccata gaagaggtaa gcaatggatg cttttctcct tgtatcacag gtgaaagata
    19261 acgatcaact aacgttctga cttggtccat ataaagttct aagtcaggga gttcttccca
    19321 gtgaggcaga cggaatgttt ctagttcctt cccccactcc attaattgct gacgcatttt
    19381 ttccatcata accgctcctt ttttcatttc ataaacatga tatcagactt atgataaaca
    19441 ttcaatctag ttgctttaac taggtgattt gattagcggg attctatgaa aacaaaaaac
    19501 aagaaggcta agccttcttg tcttttatcg ttaagaacag gagacccgcc ggggagacgg
    19561 cgggaaagga gtaaaaaatg aaaaaagtgt tagggtttgt tctcttgcaa gaactttgtt
    19621 tgttggtatg cttatataat agcgagcaaa tgtgaataat ctatgaatga tttcctataa
    19681 ggaagtaaag atttttctga ttttctttaa atgtagtctt gcattttaaa attcttagtg
    19741 ctactattat taagtacttt ttgtacgggc cgttagctca gttggtagag cagctgactc
    19801 ttaatcagcg ggtcgcgggt tcgagcccct cacggcccat tgggtgccaa acccacggaa
    19861 caggtaaatg ttggtgcgaa agcatttcga aaacatttac gctgttcttt ttttattttt
    19921 caaaataact agggcttttt atttttaact caaagtccac tgtagccctt tggactttga
    19981 gttaaaaaag aagcataatt atgaacagta aagtttctaa tataacaata attcgagaag
    20041 ctttgcaaaa tcatgctgcc aatgaactct ctaaacatat aggattaaat ctaagtacta
    20101 taaaaggtta aaaagtgaag aatatttaat agaaaaacta aatttacttg ctgctttccc
    20161 cttttccatt aatacaacaa tc
//
LOCUS       DAZUMO010000049        19699 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000049 DAZUMO010000000
VERSION     DAZUMO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.049"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..263)
                     /locus_tag="ACG8ER_001859"
     CDS             complement(63..263)
                     /locus_tag="ACG8ER_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5050434.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(715..1035)
                     /locus_tag="ACG8ER_001860"
     CDS             complement(715..1035)
                     /locus_tag="ACG8ER_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050435.1"
                     /translation="MKKRVLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1538..2656)
                     /locus_tag="ACG8ER_001861"
     CDS             complement(1538..2656)
                     /locus_tag="ACG8ER_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050436.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPEIEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2889..3011
                     /locus_tag="ACG8ER_001862"
     CDS             2889..3011
                     /locus_tag="ACG8ER_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050437.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            3127..4434
                     /locus_tag="ACG8ER_001863"
     CDS             3127..4434
                     /locus_tag="ACG8ER_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS5050438.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGESRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            4553..5752
                     /locus_tag="ACG8ER_001864"
     CDS             4553..5752
                     /locus_tag="ACG8ER_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5050439.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5779..7047)
                     /locus_tag="ACG8ER_001865"
     CDS             complement(5779..7047)
                     /locus_tag="ACG8ER_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS5050440.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(7102..7168)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(7210..7857)
                     /locus_tag="ACG8ER_001866"
     CDS             complement(7210..7857)
                     /locus_tag="ACG8ER_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS5050441.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(8265..8768)
                     /locus_tag="ACG8ER_001867"
     CDS             complement(8265..8768)
                     /locus_tag="ACG8ER_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5050442.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8891..9655)
                     /locus_tag="ACG8ER_001868"
     CDS             complement(8891..9655)
                     /locus_tag="ACG8ER_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS5050443.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMALTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFMVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKENAESLNVGVAAGIVLYHFSL"
     gene            9763..10038
                     /locus_tag="ACG8ER_001869"
     CDS             9763..10038
                     /locus_tag="ACG8ER_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS5050444.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEEDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10715..11665
                     /gene="yidC"
                     /locus_tag="ACG8ER_001870"
     CDS             10715..11665
                     /gene="yidC"
                     /locus_tag="ACG8ER_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS5050445.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11844..12818)
                     /gene="lacD"
                     /locus_tag="ACG8ER_001871"
     CDS             complement(11844..12818)
                     /gene="lacD"
                     /locus_tag="ACG8ER_001871"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5050446.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(12815..13273)
                     /locus_tag="ACG8ER_001872"
     CDS             complement(12815..13273)
                     /locus_tag="ACG8ER_001872"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5050447.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKDVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTKIATILTKEIGEDL"
     gene            complement(13270..14736)
                     /locus_tag="ACG8ER_001873"
     CDS             complement(13270..14736)
                     /locus_tag="ACG8ER_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5050448.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDASMGMMVPVLAAYIAEGIAKRPGLIVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDDSKAANRTVIEKGRAGDLS"
     gene            complement(14718..15668)
                     /gene="pfkB"
                     /locus_tag="ACG8ER_001874"
     CDS             complement(14718..15668)
                     /gene="pfkB"
                     /locus_tag="ACG8ER_001874"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5050449.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIREERFERWENIN"
     gene            15811..16560
                     /locus_tag="ACG8ER_001875"
     CDS             15811..16560
                     /locus_tag="ACG8ER_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5050450.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINVLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISQKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            16826..18304
                     /locus_tag="ACG8ER_001876"
     CDS             16826..18304
                     /locus_tag="ACG8ER_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS5050451.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(18372..19463)
                     /locus_tag="ACG8ER_001877"
     CDS             complement(18372..19463)
                     /locus_tag="ACG8ER_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5050452.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         6084 a         4031 c         3409 g         6175 t
ORIGIN      
        1 aaaatagaca aaaacgccca gacacgcaaa tgtctgggcg tttatttttt agtataggtt
       61 aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa
      121 gttactgctt ggccttcttc taaagttttg aatccgtcgc cttggatagc tgagaaatga
      181 acgaatacat cgtttccgtt ttctgcagtg atgaatccaa atcctttttc tgagttaaac
      241 cattttactg tacctgtttc cataataaaa atcctccttg tgtatcaaca catattttgc
      301 aattacttga atggtgtata tctggaagat gtttatttga aacaagctac cacagattac
      361 cgaacaaaaa ctactcctat agtatatcat accgtttaca aaaaagctag agtttttcga
      421 ctttttttat taaaaaaagt tttctcagcg attacataaa gtgaaaaacc gaactttttt
      481 taaaataaac gaaatattgt tcagttggtg tttttttgct ctgatgttct tctttaaact
      541 aaaaaaatta ttctttctga ctacttatcg gttctgtttc aacggtcgag gctggggaag
      601 attctttagt aggtctgatg ttctcatggt atttctgata gcctatgaca gtgaccagaa
      661 gaacaaggca aaccaatacg atcaacgaga ttagtttttc ttttttgctc atatctatac
      721 ctcataaata acaggatgat gtccatcata tttctgagta tgagtaaaag caaaagctgc
      781 agctgtagaa tgtaagaaat gccagcaact ttcataatca caggtcggac agtaccaaaa
      841 gtaaccaaat cttggctgca aagaccgttc ttctgtttca ggaatcgcag aatcaagatc
      901 tgttgtctgt acataaacat ttttgtacag acgtgtgcct gtaataggag accgctgtgt
      961 ttcttcagca aaaactggct gacttgttaa acttagggat agaaacagca aacaccataa
     1021 cactcttttt ttcataaaga cctccttatc gataagtttt tcccaattaa aatcatatct
     1081 caagaacgcc cgttattcac attttgttca taaatgatgt tttttgtatc ataatcgtca
     1141 aaaagaggtc gtgaccacgt cacaacctct tttttaaatg gtgttccaga agtagcttct
     1201 tcggaaataa gccgaaattt cacaaaaatt tgagaaataa tttttgtcaa tctcttctta
     1261 tttcttgaag ctgaccactt ctgtcacaac ctcttcctta aacggtgttc aaaagcttgc
     1321 atctgcggta ataagcccac caaacaaaaa atatgaagag ctatttttgc ttgctgttct
     1381 tattacttga tgcagaacag ctttttcaca acctctcata cacggtgttt aatcagctga
     1441 ccttcggtat tagctcaaaa aacggcaaaa tatgtgaaac tatttttccg tttgtttctc
     1501 taatactcag gtcaaaacac tgttttcaca acctctttta ataacgaaga atcgctgtca
     1561 gactcttttc atctggcgca tctttttgat ccaaaataaa cactttcccg ccattaatga
     1621 tcacatcatc tgctaatgca ttcaacactt gacgccgatc atattctatt tcaggatctt
     1681 caccgaaacc atcagctaag tttgacgtag cgataaatag atgcgacact tttccttctt
     1741 tcgcagctgg aacgatatca actaactgat cgatgaattt cttatctaag agtttattgt
     1801 attcttttga ttcttgagct gcgagttcac ttgacagctt cttcgcccct tcttcgatat
     1861 ctctaaagga tagctgcgca ggagaagcag aaatcgcaac gtctgcacaa taatagggat
     1921 ttttagcaat ttttttgaac atcgtctgat tttctggtaa tgcaaataaa taaatcggcc
     1981 attgttctgg attttcgaaa gaatccttca agtacgtatc aacagcttgg taatagttga
     2041 cccaatcgat ctctacttct tcatcttttg gatttacgcc atggaaagtt actccatctc
     2101 gggctccgcc tcctccatta gaagagaagt tcaaaccgcc acctgtcagt tcagatccca
     2161 atgcagtaac gacatcaacc ggtgcccctt ctggtagaga tacttctttt acttgtttgt
     2221 tgctgacttc atacaatttc atggagtcgc ggttcaatcc caacagataa taggaatagt
     2281 tgaactgtcc atttttgata atggccagta aataaggaag atcacttaca tagtattgat
     2341 cgtctaccgg gatactcaaa cgatggatat aggtttggtc agcagttata atgaccgcta
     2401 cactcgtagt tgcactacgc caaaacgaag gatctcctaa caaattttca attttttctt
     2461 ggaacgggga ccagtcatgt tcggaatatt ttttctcaaa acgtttcttt gcttctttcg
     2521 caaaattttt gaatttgatt tgatcttttt ccacatcttg atgggcgaca tgcgtattca
     2581 gcatgatcgt gacgaatggt cctttagcgc cttctgaagt caattgtgac aataattctt
     2641 tatccttttt tgccataaac gtacccctcc taaaaaaatc attcctttgt ttctatctaa
     2701 agtctaccac aacactattt ttccatacaa taaaacgtat ctaaaaaaat aaaacggttt
     2761 ctacggtttt aaatttagct atttttttaa gttatcttca taatttcctc aattatttag
     2821 ccccagtctt caactattct tcatgaaatg ctgataaact aaaaaaaaga ataaaagagg
     2881 tggatcttat gcatttgatt atgattttca gtttattaag tattatcagt ttatttgctt
     2941 tcagcgcttc ccgcttatta gatgcggaaa cacagcctat tcctgtaaaa gtacgtaaaa
     3001 acgaccgcta accttccata acgtgcgttc ttatcctaaa gttttttcag aaacattgaa
     3061 actctctccc ctctctgata aaattttaat ttgtagcgaa acaaattaat tacaaggaga
     3121 tcggatatgt ccataaaaaa aacacgtcta gtcctattat tggctagtct cattctttgc
     3181 cttgcttctt ttctaccagc tgttgctgtg caagctgaag atacgttcaa agtaaatgca
     3241 aaagcagctt ttgctgtaga tgcgcaaagc ggtaagattc tttatgatca agatggagaa
     3301 aaaaccatgg ggatcgcttc aatcacaaaa atcatcggtc tttacatcgt tcttgatcaa
     3361 gtaaaagaag gaaaactatc atgggatgat aaagtttcga tttcagatta tgcagaaaat
     3421 ttgagtatca ccccagattt atcgaatgtt cctttgcata aagaaaacac ctacactgtc
     3481 aaagaattat tcgactctgc aatcatccag tcagccaatg cttcaatggt ggccttggca
     3541 gaaaaaatct ctggcagcga agctaaattc actgaacgaa tgaaagaaca attgaaagat
     3601 tggggaatca aagatgcaac gatcgttaat gcatcaggac tgaataactc ttatcttggc
     3661 gaaagccgac cagaaggtac tggagaaaac gacgaaaatc aaatgtctgc tcaagatgtg
     3721 gccatcgtgg cccgtcattt gatcttggat tttccagaaa tactggacgt atctagcact
     3781 acgacacaaa tgtttggcga aaacacgcaa tcaccagtgg aaatggtcaa ctggaactgg
     3841 atgctcccag gattcgttaa ttacaaggaa ggcgtggacg gattaaaaac tggtacgact
     3901 gagcttgcag gtgcttgttt cgttggtacg atcactaaaa acgatcaacg gatcatcact
     3961 gtcgtattga atgcggacgg ccatgcggaa aacccgagtg cccgcttcaa tgaaacagac
     4021 cgtttgatgg actactgcta tgacaattgg tcagaaaaag agctcggaaa agctaacgca
     4081 agtattccta gcctaaaaac cattgatgta aaagacggaa aagaatcaac agttcctgtt
     4141 gtgcttaaaa gccctgtaaa agtatgggta cgaaacgata tggatactgg caaattaacg
     4201 atcacgccaa cacttgacaa gaagcaagtc actgacaacg agatccaagc acctatggat
     4261 aaaggtacaa aaatcggtac cgctacgatc accttggcag acgataaatt aggctattta
     4321 gaagacaaca atgcgccatc aacagagatc attacggata aaacagtgga aaaagcgaat
     4381 atgttcgtga ttggctggcg acgcgtcgca gaatttttcg gcaacttgtt ttaattgaac
     4441 aataagaaaa gcatctttcc atcggaaaga tgtttttttg ctttttctag tttaaataac
     4501 agcttttttc atctattttc gttatactgt ataagaaaaa ggagttgaat ttatgaatct
     4561 aaccagagat ctgatctttt tagttctcta tcttattggg gtattctcta tcgtttattg
     4621 gcgaaaacag aagaaggatc cttccttgtt cttttacggc agtacgtgga catttacggt
     4681 aatcttaact tattttgtcg tgttggaaat tttttattcg accttttatt ttttgattcc
     4741 attgttgttc tttagtatct ttgctttttc ttatttcaca gaaaaacgaa ggctgctgaa
     4801 tggtctttta ttcaatgttt tcctcatcag tttcggcatc tacttgtttg tcttgctgta
     4861 tgaaacgcaa aatattttcc ttggcggact gatcgccttg atcacgattc cattgctact
     4921 tgtcctttta tttggtattt atggattgat tgtttttctt ttttggaacg gggtcacagt
     4981 tttacgtaga gaatcgcatt cattagcgaa tcttttaacg ttgatactag ccatttttct
     5041 tacactgttt cttgtttttg actttttctt gttaaaatat ttgccgcaat ggataaatgc
     5101 tttgtttttc tgcgttcccc tgatcttgat ctatctattt atcgtattct acaacttttt
     5161 gactgtctca ttcttgtacc agttcaaccg gcctcgatac aatcaagact tcatcgttgt
     5221 gttaggcgct gggttgatca atggagaaac agtttctcct cttctagcca agcgaatcaa
     5281 taaggcaatt gctttttatc gggctcaaag ccgtgctaca ttaaatccgc ccatcttatt
     5341 gatgtctggc gggcaaggag cagacgaaaa agtaccagag gccattgcga tgaaacaata
     5401 tgccatggaa caaggaatcc ctgaaagaga cattcttgtc gaaaccaatt cgacaaccac
     5461 tttggagaac atgctctatt caaaggaaat catggatcag caaatgaaag ggccataccg
     5521 cgcgatcttt agttccaaca attaccacat ttttagagcc ggtctttatg cgcggcaagc
     5581 caaattgaaa gcaaatggta ttggtgcaaa aacagctttc tactacttgc ctaatgcgtt
     5641 tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa agacatatga tcatttgcgg
     5701 attgcttttc gcaggctccg cattcttatc gctgattact ttacttttct aaaaaaacaa
     5761 ggtgtgacaa aacatatgtc acaccttgtt tttttctttc attctcttaa ctattggcaa
     5821 aaagagtatc ccttttttga ccgctacttg ctgagcagaa taaataccgc tgttgcctga
     5881 acacataaag ctgatcaagc agatcataaa gaagtaaaca gccgcttgtg aaccgaacaa
     5941 ttcgatcccc atcacaaagc aggcaatcgg tgtatttgtc gcaccagaaa atactccgat
     6001 aaagcccaat cctgcgagaa aaggaacaga aacatggagc aaaggcgcta gtgctgcacc
     6061 aagcgttgca ccaatttcaa acaatggtgt cacttctccc ccttgatatc ccgcgcctaa
     6121 agacaaaact gtgaagaata acttcccaat aaaatcatag acatgtgcct gtccattaaa
     6181 tgattcttga agtaacggaa gactcaatcc gagataacgt tgtgtttgca aaatcaatgc
     6241 tgctgccaca actacagccc cacctatcgt atttcgccaa acaggatgag gaatccattg
     6301 tgcataccgt gcttttaaaa agacgatcga acggctgaat acccagccaa tcaaaccaaa
     6361 agcaatccca gcaacaaaaa ctttgaagaa cagcaacata ctccattcag ggatcacacc
     6421 catttggtaa tgtaggtgag aaacaccata gctttccgtc atgaaatttg caaacagtgc
     6481 agcaaagaag ctagggaaga tcgcttctgt tcggactttc ccgattgcca acacttctaa
     6541 tccaaataat gttccagcta aaggtgtgcc aaatacagaa ctaaaacccg cactgattcc
     6601 actgataatc aagatttccc gttctgcttt atccaatcgg aacaaacgtg ctgtattatc
     6661 tgctaacgct ccccccattt ggactgctgt tccttcacgg ccaacagatc ccccaaataa
     6721 atgggtagtt atggttccaa acagtgtaag tggaataagc cgcaacggga ttttttcttc
     6781 tccaccattt ccttgatcga tcactaagtt gtttccacga ctagaaagtc cgccgtgata
     6841 cgcatagaga taagcaaaaa gcgcaccact aactggtagt agataaagga gccaaggatg
     6901 tgatagtcgg atatctgtca caactgtcaa gctatttaag aaaaaagcag acaagctccc
     6961 catgaatata ccgagaatcg ctgtgatcac cagccacttc agcatgtaca ctaaaatgcg
     7021 tactggactc ggtaattgtt tttccataaa aaattcctcc tcaataaaaa aacgcctact
     7081 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
     7141 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
     7201 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
     7261 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
     7321 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gaaacattcg atgttttctg
     7381 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
     7441 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
     7501 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
     7561 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgaa cgatttgctt
     7621 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
     7681 taaaaatgta tcgatttctg gcagttcgtg atctaatatg tctcgaatct tttcagctgc
     7741 tagtgcctga actgtttgca ccgtttttgg atcattgaca gatgagtacg catttaataa
     7801 ctgttctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
     7861 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
     7921 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
     7981 tcaccccctt taatgcttat tcttctatgt acatgattca ttcgataaat aaaagtaaac
     8041 cgtatcttga acagatagtc aactgcaaac agaaaaaagg actgtgacaa aagtcgtgtc
     8101 aaagtccctt gtttcgaatg aatggtgttc aaaagcttgc atctgcggta ataagccgaa
     8161 acttcacaaa aatttgagaa gtaattttcg taaattcctt cttatttctt gaagctgacc
     8221 acttctgtca caacctcaat atggtctatt attggtttta cctgttaaat cgaggattct
     8281 ttcccaaagt aacgttgcca tctcttacgc atacttgcag acatgggctg tgccgcttct
     8341 ttgactgcgc agtatttgtc cacgaatgtc acgatatatc cttctttata cttaggcggt
     8401 gcgatcgttg caccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg
     8461 gtgatttttt ctgcattctt tacagcaatt cttggatgga tatacgcatg tgtcccctcg
     8521 tcaaatttcg tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct
     8581 gttgctttag cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg
     8641 atggaatgct ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact
     8701 gcttctgttt ctaaaagatc ttgtacataa gacatatatt cttcgtcttc acgccatttt
     8761 tctgtcataa ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta
     8821 agtataccac cagtgttcgg attattctag tcgctctaga taaaatagct aaagcttaat
     8881 agttccttaa tcataaagaa aaatgataaa gaacgatccc tgctgcgact ccgacattca
     8941 atgattccgc attttctttc attgggatat atacgttctg atcagtcaaa gacaagattt
     9001 cctgacttac tccctgacct tcgtttccca tgattatagc gaaatcctct gttttttcta
     9061 ctttgtctaa tgcatgtgct tcttcattta attctgtacc gtacaccata aaccctttgc
     9121 ctttcattac agggatcact tctgccaaat ctttttgtaa cacaggcaaa tgataattgc
     9181 ttccctgcat gcttcgcagc acctttgtac tataaatatc tgccgttcct ttacctaata
     9241 aaacaccagc cataccaaac gcatctgccg tacggatcat cgttcccaca tttccaggat
     9301 cctgtacatt atctagtaaa agccagcctc ctgaaaaatc tggcgtatct tcagatgtta
     9361 acgccatcac tgctaagatc ccttgcggtg taggtagatc tgacatagcg gtcatgacct
     9421 catctgaaac tagaaaagta gaaatctctt tgtttacagc cagccagtct ccccattctt
     9481 tcatacctct ctgtgtcaca aaaagttctt ttatttctgc atcagacgac gcagcttctt
     9541 cgatcagatg gaacccttcc aaaagatatt ctgccttttc ttctctgtat ttctttttat
     9601 gtagtttttt taattctttg attcttggat tttttgctga ttggatctct ttcacattcc
     9661 ttcacctcac ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat
     9721 cggtatcatt gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg
     9781 aatgtccaag gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat
     9841 cagttaggcg tgaccggaac ggtaaaaaac gaagaggacg gctcagtcac aatcgaagca
     9901 atcggaaatg acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca
     9961 agtggacgag tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa
    10021 tttgatgtgg tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt
    10081 ttgtgaaaat gtctttccac atttttgcat aaatttgact taataccgag aaactgactt
    10141 ttgaacaccg tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca
    10201 ttttcaaaat agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat
    10261 aaaacaccgt caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg
    10321 tgaaaaatag ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt
    10381 tgaacaccat cgataaggtt gtttttgagg cactttgcct caagtattaa agacaggtag
    10441 taaaaaagct gatctataac ttgcaatgaa tttgcttaac ccatattttc cagacagaac
    10501 aaaaaagttt ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta
    10561 agtatttctt tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaaagaa
    10621 tatttatatg aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa
    10681 gtttgtgtaa aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt
    10741 gggatccgga cttgtcagta tcctgctctt tttatctggc tgcgtgcgga tggattcaaa
    10801 cggaaaccct gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc
    10861 gattacatat ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac
    10921 tgtgatcgtt cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat
    10981 ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc aaaagctaaa
    11041 agctgcaaca acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg
    11101 cgaaaacgga ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc
    11161 gatcttctct gccttgtatt ttacggctcg ctacacagaa ggtatccgtg aatcatcttt
    11221 ttacggcatc gatctaggct caccaagtat ggtgcttgtt gccattgcag gggtcgctta
    11281 tctgcttcaa ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg
    11341 tacgatgctg atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg cgccagccgg
    11401 tgttacactt tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa
    11461 cgttatgatg aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc
    11521 aaaacaagtc gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa
    11581 acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc
    11641 aggaaagcag cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca
    11701 gttttagcaa ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt cgatattttt
    11761 atttaagtga atgtttaatt tgaatcatcg attgatcgct atctaattgc agcagcatct
    11821 ttttatgaag aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttcttct
    11881 aatagtgtac ttaattcctt aatattttgt tttccttctt ctaacatcca actactcgct
    11941 ttttcttcgc cttcggtaac aaaaggcgcc actcccttcg cccatgttgc tcgcccacat
    12001 aagacaccat tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaatagc
    12061 gtagcattga ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca
    12121 tagaaatacg cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca
    12181 aatctcatat tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa
    12241 aattctcgca tggcttcaat aactttatgt ggttttactt ttgcataggc aagagaagac
    12301 ggatcgccaa tagttgcatc ataagagagg atctctaaaa agaaaggtat gccttctgct
    12361 tcgcattctg aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt
    12421 tcatcaacgt catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt
    12481 ttcaccgacc aaatattcaa taaatcaggt aatcttcccg gaattttcgt atcatagccg
    12541 gttttttcat aagctagcag caagccacat tctttctctc gaagcgctgc tgcaggcagg
    12601 ccgtattctg gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc
    12661 tgtttaaatt ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc
    12721 aaagcaccac gctggtcgat tgctaacgcg ctaataattc cagcttcatc cgtcaatcgc
    12781 ttcaaacgtt cctgcttttc tatactcatc ttcttcatag atcttcacct atttctttag
    12841 ttaatattgt ggctattttt gtttttgatt ttgcttttct caactgttct gtaacctctt
    12901 ttcgcatcaa tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg
    12961 aatctggaat aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt
    13021 caattccttt gttcagtgtg acgataatca tactaggttc gttgattgtt tgagacttcg
    13081 catgaggaat agcaaaacct gacattattc ctgtagtacc ttctttttct ctttctagaa
    13141 gtttttgata tacatctttt tcgtcatgag caattcctaa gcagaatgtt tgctttgcta
    13201 aaaacttaaa tgcttcttct tgactatcca atggctgttg taaaaaaatg ttctcttcat
    13261 gaatgattgt catgataagt ctcctgctct tcctttttcg ataactgtac gatttgctgc
    13321 tttactatca tccatcttct tgtctttatc agttactctt ttcttttgga ttaatcctag
    13381 tatcactcca ccaacgatcg aaccaataag aatcgctgta acccattgga tttttcctgt
    13441 tactacaggt aatactaaaa aaccgccatg tggtgctgga acttggacgc caaacaaata
    13501 agtcaagaca gcagcaattg atgagcctaa catcatcgtg ggtaacactc ggatcgggtc
    13561 tttcgcagca aacggaatag ctccttccgt aatatgagtt gatcctaaaa taaaattaac
    13621 caaacctgca ttacgatcat tggtatcaaa atatttacca aacaataaag tagcaaaacc
    13681 agtaatcaac ggtggcgcaa tacaagctgc tgacacacca gccataaaag tcgtgtttcc
    13741 ttgtgccaaa agagctgttc ctgttacata tgccgcctta ttaaccggtc ctcccatatc
    13801 aaacgcacac atgcagccaa caatcacgcc aagaacaagt ggattcgaat gttcaaatcc
    13861 agctaaaaag ctcatcattc cttcatttat agccttcatt ggacctgcca gcaaagacat
    13921 cacgatgcca ataaatgcta cgccaataac tggatataag aagattgcct tcaagccatc
    13981 tagttgttta ggtaaaattg tcagtagctt ttgcaaaaat aagataaaat aacctgctaa
    14041 gaacccagct aaaatacctc ctaaaaatcc agtaccgcca ttcatggcta cgattccacc
    14101 aacaaatcca acgattaacc ctggacgttt cgcgatccct tctgcaatat aagctgctaa
    14161 aactggaacc atcattccca ttgatgcatc gccaactgct tttaccattg ccgctgtagc
    14221 attgtattgt gcatgatctg gatcaaaaga atagattccc caaacggcaa atgaaatagc
    14281 aatcaatact ccgccagcta ccacgaaagg caacatatga gagacaccgt tcatcaaatt
    14341 tttatagatg ctacgaccaa aattagcacc actcatcgct tctggttcta aagtatcagc
    14401 agcttcatta tctgctaaga cagtaccttt tcctgccaat gcttcgtcaa tcagccgttg
    14461 tgcatccttg attcctgtac tgacagaaac atcaatgact cgttttcccg caaatctttc
    14521 tgaatggaca tctttgtctg cggcaataat gacagcatct gcctgtttga tttcttcagc
    14581 cgttaattca ttttcgatcc caatttgtcc atgggtctct actttgatcg tgatgccttt
    14641 tttcctagct gcttgttcta atgcctcttg cgccatgtag gtatgcgcaa ttcctgtcgg
    14701 acaaccggtt gctgcaatta gttgatattt tcccatcttt cgaacctctc ttccctgatt
    14761 ttgatttgtt tacttaattc ctccacatct aaaaaatctg ttaatccttt gcgaaaagcg
    14821 gttgagctgc ctgctgctat gcttttacgt aacgtttcat caagcggtcg tcgattcatg
    14881 tatcctgcta aaaaagcgcc taacatggta tcacctgcac atgctgtatt gacaactttt
    14941 ccttttggcg agtttcctcg aaacaccctg tctttcgtaa aaagaattgc tcctttctca
    15001 ccgagggata acaaaacatt ttctgcacct aactctacta gtcgttgtcc ataaacaaag
    15061 taatcttcat ctgtaaccat ttttcgtccg aaccaagaag aaagttcttc ctcgttcggt
    15121 ttcagcaaaa atggtcgata gggtaaacaa tccatggctt cttgatcact actatcaata
    15181 atcaaatgaa tccccttttc ctgacaaatc cgactgattt ctattaaaac ctttggagac
    15241 agccctcttg gtaaactccc agaaacacaa agataatctt ctgcttgtaa tgctcttatt
    15301 tgatttaaaa aattatggac agctgtctgc ggtatttcag gtccttgatt cactaatttg
    15361 tattcttctc ccgtttgatt gacttgtgta aatacattga tacgcgtcat accaggcact
    15421 tcaataaact ctgtttggat ttgtttctgc tgtaaatagt cttctatata ctttcctgtg
    15481 aacccagcag aaaaacctaa tgccgtactt gggaaatcta gcatctttaa tactagtgac
    15541 acattcactc cttttccgtt tgcctgaatg tcatcctctt ttgtgcggtt taccataaaa
    15601 ggatgcatct cctctgtttc aataaataga tcaattgcca aattcatcgt gcatgtgtag
    15661 atagacataa ccaatgacct ccttgactct attatgaaat acgcgaaaga gaaaatgttt
    15721 tcttaatcgt tccaacacaa agaaaacctt tacatttata aactcgtcgt gctatttttt
    15781 aactaacttt tggtgaaaga aggaaagaat atggatcaca aattaagtga aacagaacag
    15841 tacctctgga actttatcga acaccatatc ctagagatcc ctaactactc catcgtgaaa
    15901 ctaagtgaac aagcaaatgt ctcaacagcc accattgtgc ggacaatgaa aaagaaaggc
    15961 tatgaagggt ttacttcgtt taagcatcat ctaaaagaag gagaaaacaa gaacatcaat
    16021 ttttctttct tagataaagt agacaaaggt atccgccgag ccattctaaa aaatgaacaa
    16081 gaagtggtac gtacgatcaa tgtgttggaa atcggaaata ttgaggatgc gatacaaaga
    16141 atcaaatttg ctgaccgggt ttttgtcttt gcccgaggat tttcagaaat gattggccaa
    16201 gaaatgctgg taaaacttca attaacggga aaaaattgtc aaatgcatac tgatccagaa
    16261 attatcaaaa gcatcagtca gaaactgact aagaaagaca gcgttatttt tgtttctttg
    16321 aacggcgaga caaaagaact cgtcaccgcc gcaaagaatt gctatgatgc agagattggt
    16381 tcgattctag ttacagccaa tcattcctct acactgaaag actattgcga gatcaatctc
    16441 gtaggtttca aatcagaagg ttcttatttc cctgattatg aagtacggtc acgcttgcct
    16501 ctccagatta tcgcgagaat tctgcttgac gcctatgtat tgcgtatggg ggagaattga
    16561 gaagcagttt attatggttg gttacagttt aaaacatcac attttttatt ctttatattg
    16621 tatagactca cgtaaacttt ttatgtataa agttattcac aatctataaa gttactttaa
    16681 gtaacaaaaa ataattgaaa atacttaaaa tcaaaaaata tctttttaat caataaaaaa
    16741 attgacagtt ttattaataa taagctattc tgttatagaa gaaaaaattg tttgttatta
    16801 ttagcaacaa aaaaaggagt gcatcatgaa aaagttatct caaaaacgtt tagctcaaat
    16861 cattattgat tctagaaaat ctctgaaaat gacacaacaa caacttgcag tagctactgg
    16921 tatcaaccgt gccttaatta gtcgattaga atctctcgat tttatcccgt caatacaaca
    16981 attagaatca ttagcagaag ttctaaattt tgaaccaaca gatctattta taaatgaatc
    17041 aaataaatca aacagaacaa aacatcagtc gaagaatatt gctgtagccg gtactggtta
    17101 cgtaggtctt tccattgcta ctcttcttgc ccagcacaat caggtaacag cagtagacat
    17161 tcttcctgaa aaagtaaaac aaattaacaa taagaaatct ccaatacaag acgattacat
    17221 tgaaaaatat ctagctgaaa aaaatcttaa tttaattgca acgactgatg gtgcagccgc
    17281 ctatagagaa gccgattttg ttgtcattgc tgctccaaca aattatgata gtaaacaaaa
    17341 ttattttgat acttctgctg tagagtcagt aatcaaattg gtgttagaag tcaatccaaa
    17401 ggcaataatg gtcatcaagt cgacggtacc cgtaggctat accgaaagta ttcgtaaaaa
    17461 atacaatact aaaaatattc tattcagccc agaatttctc agagaatcta aggcattata
    17521 tgacaatcta tatccttcgc gaattattgt cggcacggac caaactgaca aaactttacg
    17581 agaagcctct cacacttttg cagatttact ccaagaagga gcaatcaaaa aagatatcga
    17641 aactcttttc atgggattca cagaagctga agcagttaaa ttattcgcta atacttacct
    17701 tgcacttcga gtatcttact tcaatgagct ggatacttat gcagaaatga aaggactaaa
    17761 tacacaagaa atcattcacg gggtttgctt agatccaaga attggcacac attataataa
    17821 tccatctttt ggatatggag gctattgttt accgaaagat acaaaacaac ttctcactaa
    17881 ctataacgat gttcctcaga atatgataag tgcaattgtc gatagtaatc gtacaagaaa
    17941 agactttata gcagatcgag tgttagaact tgctggcacg tatgaggcta acagttcgtg
    18001 gaatgcagaa aaagaaaaag aggtagtcat aggtgtatac cgtcttacaa tgaagagtaa
    18061 ttcagacaac ttccgtcagt catctattca aggagttatg aagcgaatta aagcaaaggg
    18121 ggcaactatt gtaatttatg aaccaacact tgaaaataac actacatttt ttggttcaaa
    18181 agtaataaat aatttagatg attttaaaaa tatcagtcac agtattattg ctaatcgtta
    18241 cgatagtaat cttgatgatg taaaagaaaa agtatatacc agagatttat ttcaacgaga
    18301 ttaaaaaata acacctcaga tttgtagtgt ctgaggtgtt tctataaact tttataagta
    18361 aaaaaataca attatacttc ataaaaagac ttataccact gcgcaaattt acgaagcccg
    18421 tcacgcaaac ttgtacttgg tttaaattca aaatctcgtt ctaacgcact agtatctgca
    18481 taagtaactg gcacatctcc tggttgcatt ggaactagtt cggtatgaga ttcaaagtcg
    18541 taatcttctg gtagcacccc tgctgcaatt agctcttgtt gtagaatatc tacaaaatct
    18601 agtaggtttt ccggcgtatt gttaccaata ttataaatat gatatggtgg gattggtagc
    18661 ccatcttccc catcaatttt ctctggagcg cactgcataa ccctctttac tccttcaaca
    18721 atatcatcta tataggtaaa gtcacgcttg caattcccat aattgaatat ttggatatta
    18781 tcaccagcac gaagtttatc agtaaaacca aaataagcca tatctggacg cccagcagga
    18841 ccatatactg taaagaaacg taaccctgta gacggaatat tgtaaagttt agaatatgca
    18901 tgtgccattg attcattact tttcttagtt gctgcataca acgatacttg attgtctact
    18961 ttatcctctg tagaataagg aattttctta ttagtaccat aaacggaaga cgatgaggca
    19021 tacactaaat gttccacacc agattctcca ttatcatatg aatgacgaca agcttcaaga
    19081 atattataga atcctattat gttagattcg atatacatat ctggattagt aattgaataa
    19141 cggactcctg cctgagctgc taaattcacc acgatacttg gtttataatt atcaaagatg
    19201 aaatcaattg ttgctttatc agcaatagaa cctttgataa aattccaagt agatcctgga
    19261 tggttctttg cctccgtttc tatctctgat aatctatagt tcttaataga gacatcatag
    19321 taatcattca tattgtcaat gccaatgatt tttattgatg actgagtacg taataactca
    19381 agaaccaaat ttgatccaat aaaaccggct gcgccagtta ttaatattgt tctatttgat
    19441 aaggacgtgt ttaattccag cattttttag cactcacttt ctttttttct tatgaataca
    19501 tatttgttat atattaaaaa atcttattta gacttatcta ttgtcacttt tatcattcta
    19561 atctttgcac gtagtagaat aattatacag ataggaaatt gctaagcaac ccacttcttt
    19621 ctattatcta ttaacttatt ctaaaacgaa tagaaattat tcttgatcac ataagtaacc
    19681 ttaaaagaaa aaatttaat
//
LOCUS       DAZUMO010000050        19480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000050 DAZUMO010000000
VERSION     DAZUMO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.050"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8ER_001878"
     CDS             133..3660
                     /locus_tag="ACG8ER_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS5050453.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8ER_001879"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8ER_001879"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS5050454.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8ER_001880"
     CDS             7448..7789
                     /locus_tag="ACG8ER_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5050455.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8ER_001881"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8ER_001881"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS5050456.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8ER_001882"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8ER_001882"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS5050457.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8ER_001883"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8ER_001883"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS5050458.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8ER_001884"
     CDS             13000..13737
                     /locus_tag="ACG8ER_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5050459.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8ER_001885"
     CDS             13751..14566
                     /locus_tag="ACG8ER_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5050460.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8ER_001886"
     CDS             14579..15226
                     /locus_tag="ACG8ER_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5050461.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8ER_001887"
     CDS             15242..15898
                     /locus_tag="ACG8ER_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5050462.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8ER_001888"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8ER_001888"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS5050463.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8ER_001889"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8ER_001889"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS5050464.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8ER_001890"
     CDS             18240..18758
                     /locus_tag="ACG8ER_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5050465.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8ER_001891"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8ER_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS5050466.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6551 a         3344 c         4182 g         5403 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DAZUMO010000051        18504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000051 DAZUMO010000000
VERSION     DAZUMO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.051"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8ER_001892"
     CDS             99..746
                     /locus_tag="ACG8ER_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5050467.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8ER_001893"
     CDS             1139..3784
                     /locus_tag="ACG8ER_001893"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS5050468.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8ER_001894"
     CDS             4094..5407
                     /locus_tag="ACG8ER_001894"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS5050469.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8ER_001895"
     CDS             5397..6050
                     /locus_tag="ACG8ER_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5050470.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8ER_001896"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8ER_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS5050471.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8ER_001897"
     CDS             6947..7147
                     /locus_tag="ACG8ER_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5050472.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8ER_001898"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8ER_001898"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5050473.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8ER_001899"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8ER_001899"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5050474.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8ER_001900"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8ER_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5050475.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8ER_001901"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8ER_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5050476.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8ER_001902"
     CDS             13269..13466
                     /locus_tag="ACG8ER_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050477.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8ER_001903"
     CDS             13619..14104
                     /locus_tag="ACG8ER_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5050478.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8ER_001904"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8ER_001904"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5050479.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15502
                     /locus_tag="ACG8ER_001905"
     CDS             14981..15502
                     /locus_tag="ACG8ER_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050480.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDV"
     gene            15599..16336
                     /locus_tag="ACG8ER_001906"
     CDS             15599..16336
                     /locus_tag="ACG8ER_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5050481.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8ER_001907"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8ER_001907"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5050482.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8ER_001908"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8ER_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5050483.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEIAVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt gaagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattgct gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZUMO010000052        18107 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000052 DAZUMO010000000
VERSION     DAZUMO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.052"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8ER_001909"
     CDS             complement(164..388)
                     /locus_tag="ACG8ER_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS5050484.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8ER_001910"
     CDS             complement(586..2166)
                     /locus_tag="ACG8ER_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS5050485.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8ER_001911"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8ER_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5050486.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8ER_001912"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8ER_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5050487.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8ER_001913"
     CDS             5331..5765
                     /locus_tag="ACG8ER_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5050488.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8ER_001914"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8ER_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS5050489.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8ER_001915"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8ER_001915"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS5050490.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8ER_001916"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8ER_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS5050491.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8ER_001917"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8ER_001917"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5050492.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8ER_001918"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8ER_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS5050493.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8ER_001919"
     CDS             11001..11471
                     /locus_tag="ACG8ER_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS5050494.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8ER_001920"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8ER_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5050495.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8ER_001921"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8ER_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5050496.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8ER_001922"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8ER_001922"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS5050497.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8ER_001923"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8ER_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS5050498.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8ER_001924"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8ER_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5050499.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8ER_001925"
     CDS             16164..16967
                     /locus_tag="ACG8ER_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS5050500.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8ER_001926"
     CDS             17145..18092
                     /locus_tag="ACG8ER_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS5050501.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5438 a         3631 c         2964 g         6074 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaata
//
LOCUS       DAZUMO010000053        17735 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000053 DAZUMO010000000
VERSION     DAZUMO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.053"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..836
                     /locus_tag="ACG8ER_001927"
     CDS             24..836
                     /locus_tag="ACG8ER_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS5050502.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            833..1633
                     /locus_tag="ACG8ER_001928"
     CDS             833..1633
                     /locus_tag="ACG8ER_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS5050503.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1794..3062
                     /gene="rseP"
                     /locus_tag="ACG8ER_001929"
     CDS             1794..3062
                     /gene="rseP"
                     /locus_tag="ACG8ER_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS5050504.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3130..4839
                     /locus_tag="ACG8ER_001930"
     CDS             3130..4839
                     /locus_tag="ACG8ER_001930"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS5050505.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5051..9403
                     /locus_tag="ACG8ER_001931"
     CDS             5051..9403
                     /locus_tag="ACG8ER_001931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS5050506.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9547..10020
                     /gene="rimP"
                     /locus_tag="ACG8ER_001932"
     CDS             9547..10020
                     /gene="rimP"
                     /locus_tag="ACG8ER_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS5050507.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10044..11219
                     /gene="nusA"
                     /locus_tag="ACG8ER_001933"
     CDS             10044..11219
                     /gene="nusA"
                     /locus_tag="ACG8ER_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS5050508.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11241..11534
                     /locus_tag="ACG8ER_001934"
     CDS             11241..11534
                     /locus_tag="ACG8ER_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS5050509.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11531..11842
                     /locus_tag="ACG8ER_001935"
     CDS             11531..11842
                     /locus_tag="ACG8ER_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS5050510.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11855..14161
                     /gene="infB"
                     /locus_tag="ACG8ER_001936"
     CDS             11855..14161
                     /gene="infB"
                     /locus_tag="ACG8ER_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS5050511.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14188..14535
                     /gene="rbfA"
                     /locus_tag="ACG8ER_001937"
     CDS             14188..14535
                     /gene="rbfA"
                     /locus_tag="ACG8ER_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS5050512.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14643..15147)
                     /locus_tag="ACG8ER_001938"
                     /pseudo
     CDS             complement(14643..15147)
                     /locus_tag="ACG8ER_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15312..17675
                     /locus_tag="ACG8ER_001939"
     CDS             15312..17675
                     /locus_tag="ACG8ER_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFS5050513.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6046 a         3147 c         3907 g         4635 t
ORIGIN      
        1 ttttaagtat aggaggaaaa agaatgttac gtttttttcc gcaaaaaaac aaatatattc
       61 aagaaaaaag cgaatttcaa tttaataccg atggaccaat tcctcagcac attgccatca
      121 tcatggacgg caatggtcgt tgggcgcaaa accgtcgttt gccacgagtt gctggccata
      181 aagaaggcat ggaaacagta aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag
      241 tgttgacgct ttatgccttt tcgactgaaa actggaagcg accaaaagat gaagtaagtt
      301 ttctcatgca actacctgtc gacttctttg atacgttcgt accagaattg attaaagaaa
      361 atgtcaaagt tcatgtcatg ggttatgaaa acgtattgcc agaacatacg caagatgctg
      421 taagacgtgc aattgagcaa acaaaaaaca atactggaat ggtattgaat tttgctttga
      481 attatggaag tagagcagag atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa
      541 aaggagaaat acacgcagaa gaaatcgatg atgaattgat tgctaagcat ttaatgacag
      601 gtttcttgcc taaagagtta caagatcctg agctgatgat ccgtacaagt ggagaagagc
      661 gaattagtaa ttttctgtta tggcagatcg catatagtga gctgtatttt acaaaagcgc
      721 tttggcctga ttttgatggt gcgcatcttg aagaagcaat cgcctcttat caaaatcgag
      781 accgtcgatt cggcggtgta aaaaaaacag aagatgagaa aggggatcag tcatgaaaca
      841 acgtgtgatc acagcagctg tggcgctagt cttgtttatt ccgattatat ggatcggagg
      901 catagcggtt gagcttacag cagcaatctt agcggtagta ggagtttacg agctgttccg
      961 gatgaaagga ttgacactct taagttttga aggagtttta tccgccgttg gtgcagtgtt
     1021 acttgttttg ccaaaagaaa gatggttttt ctttcttcca gagaatgtaa gtacatttat
     1081 gttattttat cttatagtta tgattctttt aggaacatca gttatttcta aaaatacgta
     1141 tacgattgat gaagcaggtt ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca
     1201 gaattttgtc aatgcacgaa cagaaagctt ggctgttttg atatttggtc tatttattgt
     1261 ttgggcaact gatatcggag catatatggt tggtcgacgt tatggtcagc ataagttaag
     1321 tcctgatatt tcgcccaaca aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt
     1381 tgtagtcgca tttctttatt tattgctata cccagcaaaa gaactattca atcatggtat
     1441 gtttgtcatg cttctgatga cgattatttt ttcagcagtc ggacaatttg gtgatttggt
     1501 cgaatctgca atcaaacgtc attatgatgt gaaagactcg ggaaatatct taccaggcca
     1561 tggtggaatc ttagaccgtt tcgacagttt gctgtttgta tttcctatca tgcatttatt
     1621 tggcattatt tagtaagtat acctgcgttt ctttgtgaat ttcataaaag acgcaggttt
     1681 ttatttttca ttatattgat actattggtg tctttggtat actatggtaa aattatagtg
     1741 ttttatcgtt gttttataaa aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa
     1801 cgattctgac atttatcatc gtttttggta tattagtgat tgttcatgag tttggtcatt
     1861 tcttctttgc gaaacgatca ggaatcctcg tccgcgaatt tgctatcggt atgggtccga
     1921 aaatttatgg gcatcaagca aaagacggga caacatatac gcttcgccta ttacctattg
     1981 gcggttatgt tcgaatggca ggcaatgggg atgatgaaac agaaatggca ccaggaatgc
     2041 ctctttctct tttactaaat tctgacggca tcgtagagaa gataaattta agtaagaaaa
     2101 tccaattgac aaatgctatc ccgatggaat tgagccgtta tgatttagaa gatgagctga
     2161 ctatcactgg ttatgtgaat ggagacgaaa cagaagtagt aacctaccct gttgatcacg
     2221 atgcgacgat tattgaaaac gacggaacag aaatcagaat tgcgccaaaa gacgtacagt
     2281 tccaatcggc taaattgtgg cagcgtatgc tgaccaactt tgctggaccc atgaataact
     2341 ttattttggc aatcgtgtta ttcatcattc tggcatttat gcaaggtggt gttcaagtga
     2401 cgaacactaa tcgcgtagga gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa
     2461 aagaaaatga tgaggtagtc agtgtggacg gaaaagaaat ccattcatgg aatgatctga
     2521 caacagtcat tacgaaaaat ccaggtaaaa cattagactt taagatcgaa cgagaaggtc
     2581 aggtgcaatc tgtcgacgtg acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc
     2641 aacttggtat aaaagcccca atgaatacag gttttatgga taagatcatt ggagggacgc
     2701 gtcaagcttt cagcggttca ttggaaattt tcaaagcctt aggatcacta tttacaggtt
     2761 tcagtttaga taaattaggc ggacctgtca tgatgtacca attgtcttca gaagcagcaa
     2821 atcaaggtgt aacaactgtt attagtttga tggcgcttct ttcaatgaat ctcggaatcg
     2881 tcaatctgct tccgattcct gccttagatg gcgggaaatt agtcttgaat atttttgaag
     2941 ggattcgcgg gaaaccatta agtcaagaaa aagaaggaat attgacatta gctggctttg
     3001 gatttttaat gttgttgatg gtgctagtta catggaacga tattcaacga ttctttttct
     3061 agaaaaagtt gacaaatagg gtataataac aaacgataac tattttgagg aaataaagga
     3121 gtatatagaa tgagacaatc aaaaatgttg attccaacat tacgcgaagt tccaaatgat
     3181 gcagaagtat taagccatca aatcttgcta agagctggat atatccgcca agtatcagcg
     3241 ggtatttatt catatctgcc attagcaaat cgtgtattgg aaaaactgaa acgcattatg
     3301 cgggaagaat ttgaaaaaat cggagcggta gaaatgctga tgcctgccat cttgccagca
     3361 gaactttggg aagaatccgg gcgttacgaa acatatggac caaacttgta tcgtttcaaa
     3421 gaccgcaatg atcgaaaaat gatccttgga ccaactcacg aagaaacttt tactgatttg
     3481 atgcgtaatg aaatcaattc atataagaaa ttacctttaa atctttatca aatccaacct
     3541 aaatatcgtg acgaaaaacg acctcgcttc ggtttgcttc gcggaagaga gtttatcatg
     3601 aaagatgcat attctttcca tgcaacggaa gaaagtttgg acgagacata taaagattac
     3661 gaaaaagcct ataccgaagt attcaagcgt tgtggcttag aatttcgctc gatcatcgga
     3721 gacggcggcg caatgggcgg gaaagactca aaggaattca tggcgatctc agaaatcggg
     3781 gaggatacga tctgttattc gactgaaagt gattatgctg caaacttgga gatggctaca
     3841 agctactatg tatcaaaaaa atcgcatgaa acacagcttg aattggagaa agtagctaca
     3901 cctaacgtaa aatcaattga cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc
     3961 atcaagtctg tattgttcat tgcggatgaa caacctgtcc ttgttttggt acgtggagac
     4021 catgaagtca atgacgtaaa actgaaaaac tttttatctg ctgacttttt agaagaagca
     4081 acagaagaag aagcacaaaa atatctaggt gctgagtttg gttctgtcgg accagtcggt
     4141 gtttcagatg acgtaaaagt atatgcagac cgttatgtcc aagatttagc gaatgccatt
     4201 actggagcga acgaaacagg attccactat aaaaatgtga atccaaaacg cgatttcact
     4261 gtgctttctt atgaagatct tcgttttgta caagaaggag atccttcacc agacaacaac
     4321 ggtgttcttg cctttactcg aggaatcgaa atcggacata tattcaaaat cggcactcgt
     4381 tacagtgaat ctatgggagc aactgtacta gacgaaaatg gacgagaaaa atttgtgatt
     4441 atgggctgtt atggtatcgg tgtaagtcgt ttattatcag caatcgtaga acaacattca
     4501 gatgaacaag gaatcaattg gccaaaagga attgcgccat ttgatctgca tatcgtccaa
     4561 atgaatctaa aagacgaata ccaaacaagc ctgacgaatg aactagaaga agcgatgaca
     4621 aaagctggct atgaagtact ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg
     4681 gattctgatt tgattggctg tccaatcaga atcactgtcg gtaaaaaagc agtggatgga
     4741 atcgtagaag tgaaaatcaa gaaaactggc gaaatggttg aagtacgtaa agaagaattg
     4801 gcgaatactt taccaatcct cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa
     4861 taaaaatagc tgaatcagct gtttttgaca atctgatgat tcgcttttaa aattggaact
     4921 gcgatttgaa tcgcagttcc aattttttaa tgtaaaactc agaaaaaacg agtatactaa
     4981 aaggtactga aaaagaagag gataagatgc gatcaattgc attgtttctc atcagaaagg
     5041 gcgtatatct ttgtcagaaa aacgagattt atttgaaaaa ctgttggaac aaatccaact
     5101 agaggaagca gaaaaaaatc atccactgct tactgctggt gaaatggata gtgtagtggt
     5161 ccatcgtaaa tcgcgtttat gggaatttac gctacatttt tccaaaattt tgcctattat
     5221 gctatatcgg tcattgactc agcatctgac tatcgctttt aaagatattg cacaagtcaa
     5281 actgaatatc ttagcagatg accagacatt cgatgaacag ctcttgcaag attactgggc
     5341 ccaagctttg gaaaatcagc aatgcgatac accactagca cagcaagtat tgaaaacgca
     5401 ggtacctgtg ataaaagatc gtaaagtcat cttgccaatc gacagtacag gagctatttc
     5461 ttatttgaag caacaatact tgccattagt agaagagttg ttcgtcagtt acggattccc
     5521 aaaatttcgt atcgaaccag aagtggatga gcagcaagcg gaacgtgtat tgaaactttt
     5581 tgaagaacgc aaacaagaac aggcagaagc ctttatgaag caagcagctg aaagtctagt
     5641 tgtccacgaa caaaagaaaa aggaaagaaa agagcaatcg ccagctcttg aaggacccat
     5701 ccagctagga cgaaatattc cagccgatga accaacgaca ccaatgatca gtatcgttga
     5761 agaagaacga agagtcacac ttgaggggta cgtattcgac aaagaagtca gagaactacg
     5821 ttcaaaacgc aagatcttga ctttgaaaat cacagattat acttcatcat ttatcgtcaa
     5881 aaaattctcg aacggcgaaa aagatgaaca agtatttgat gcgatcagtg tcggtagctg
     5941 gttaaaggta cgaggaagta ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc
     6001 gcaagatatc atagaagtca aacatacacc tcgcaaagat tatgctcctg aaggggaaaa
     6061 acgagtagag cttcatgttc atagcaatat gagtacgatg gatgcaacaa acagcatctc
     6121 tgatcttgtg gcacaagcag gaaaatgggg acaccgagct atcgccatta cagaccatgg
     6181 tggtgcgcaa gcctttccgg aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt
     6241 atacggcgtg gaagcgaatg tcgtagatga tggtgtccca attgcttata atgatgcaca
     6301 tgaagcattg agtgaagcca catacgtcgt atttgacgtg gaaacgacag gattgtctgc
     6361 agtatatgac acgatcatcg agctagccgc agtcaaaatg tataaaggaa atgtgatcga
     6421 aagctttgat gaatttatcg atccaggaca tccattgtca cgaacaacgg tagatctgac
     6481 tggtatcacg gatgaaatgg ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt
     6541 ccttgaattt tcaaaagata caattttggt agcccacaac gcggcttttg atatggggtt
     6601 cttgaatact agctacgcaa ggtatggtat tcctgaagcg gccaatccag tcattgatac
     6661 gttggaatta gctagatatc tgtatcctca attcaaacgt ttcggattag gtgtgttatc
     6721 taaaaagttt ggtgttagct tggaacagca tcaccgagca atctatgatg ctgaagcaac
     6781 tggtcactta gcatggatct ttgtgaaaga ggcaatggac aatcataaca tgctttatca
     6841 tgatcagcta aatgagcata tcggagaagg agattcttac aaaagggctc gacctttcca
     6901 tgtaacgatt ttagcaaaaa atcaggcagg actgaaagat ttgttcaaac tgatttcaat
     6961 gtcgaatgtc gaatacttcg aacgcgtccc acgaatacca cgctcccaat tgaaaaagat
     7021 gcgtgaaaat ctattgatcg gctcagcttg tgataaggga gaaatattcg aagccatgat
     7081 gcaaaaaggg gtagaagagg caagaaaccg agcaaaattc tatgattata ttgaagttat
     7141 gccaaaagcg gtctatgcgc ctttgatcga gcaagaattg gtaaaaaatg aacatgattt
     7201 ggaagaaatt atccaaaatt tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc
     7261 aacaggtaat gtacattatc tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa
     7321 ttctatgggc ggagcgaatc cgttgaatcg tcatagtttg ccggatgtcc atttccgaac
     7381 aaccgatgag atgctaactg catttcactt tttaggggaa gaaacagcga aagagatcgt
     7441 agtagaaaat acgaataaaa tcgcagatat ctgcgaagaa gtgattccag tcaaagatga
     7501 actttataca ccgaagattc caggttcaga agatgaaata tcagaattga gttacacaaa
     7561 agcaaaacaa atgtacggag atccgttacc tgagatcatt caaaaacgat tagaaaaaga
     7621 gttgaattct attaatggta atggcttctc ggttatttat ctgatcgccc aaaaactagt
     7681 gcataaaagt aatgaagatg gctatctagt aggttcacgt ggctctgttg gctctagctt
     7741 tgttgcgacg atgacaggaa tcactgaagt taatccatta gcccctcatt attattgtcc
     7801 agagtgccaa tattccgagt tttttgaaga tggtacgtat ggttctggat ttgacatgcc
     7861 agaaaaacaa tgtccaaaat gtggtgcacg cttaaacaaa gacggtcatg atattccatt
     7921 tgagacattt ttaggattcc atggagacaa agtgcccgat attgatttga acttctcagg
     7981 tgattatcaa gcagaagccc ataactatac gaaagtattg tttggcgaag actatgttta
     8041 ccgtgccgga acgattggta cagtcgccga caaaacagca tatggctatg taaaaggata
     8101 cgaaagggac aataatctgc agtttcgaag tgcagaagtc gacagattag caaaaggcgc
     8161 tactggagtc aaacggacaa ccggccagca tccagggggg atcatcgtta ttcccgatta
     8221 tatggatgtc tatgatttta cgccgatcca atatccagca gacgatcaaa attcagaatg
     8281 gaaaacgact cattttgatt tccactcgat tcacgataat gtattgaaat tagatattct
     8341 agggcatgat gacccaactg ttattcggat gcttcaagat ttatcaggga tcgacccaca
     8401 aacgatccct acggatgacc cggaagtgat gcggatattt gccggtcctg aagtacttgg
     8461 agtaagtcag gaacaaattt attccaaaac agggacatta ggtattccag aattcggcac
     8521 tcgttttgtt cgagggatgt tagaagaaac gcatcctaca acttttgcag aactactgca
     8581 aatttccgga ctatctcatg gtacagacgt atggttagga aatgcggaag aattgattcg
     8641 acgaggagat gcgacactgg cggaagtaat cggctgtcgt gatgatatta tggtttattt
     8701 gatccatgct ggattagata gcgggatggc atttaaaatc atggaaactg ttcgtaaagg
     8761 acaatggaac aagatccctg atgaattgcg agaaacctat ctatcggcga tgaaagaaaa
     8821 taatgtgccc gactggtata tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc
     8881 ccatgctgca gcttacgtat tgatggctct tcgtgtcgct tatttcaagg tttatttccc
     8941 tattttgtat tactgtgcat acttttctgt acgtgcagac gactttgatc tagtctccat
     9001 gtgcaaagga aaagatgctg tgaaacaagc aatgaaagaa atcacggaca agggattaga
     9061 tgcttccgta aaagaaaaga atcaactgac agtgctggag ttagccaatg aaatgctgga
     9121 acgaggattc aaatttggaa tgattgattt gtataaatcg gatgccgtta actttgttat
     9181 tgaaggtgat acgttgatcg caccatttag agctgtccca agtttgggaa ccaacgtggc
     9241 gaaacaaatc gttgaagcaa gaaaagacgg tccattttta tccaaagaag atcttgccac
     9301 ccgcggtaaa gtttcaaaaa cattgatcga atatatgaac gacaatggcg tattgaaaga
     9361 tctaccagat gaaaatcaat tatcgttatt cgatatgcta taagcatctg ttttcctgtc
     9421 tgcttgtaat tttaagcaga gtatggtata gttcttatag gtaatgatac taactgtgag
     9481 tgagcggatt tttcgctcac tctttttaat ggaattacgc acttaatttt taaggaggcg
     9541 aaacttttga gtagcgtcgt tgaaacagtc acagaaatgg tgacaccgat tttagatgaa
     9601 caaaattttg aacttgtcga agtcgaattt gtaaaagaag gaaaaagctg gttcttacga
     9661 gtatttattg acaaagaagg cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa
     9721 ctgagtgaaa aacttgatgc agcagatcca gatccgattc ctcaagctta tttcctggag
     9781 gtatcctccc cgggagcaga gcggccattg aaaaaagaaa gcgactatga aaaggcactt
     9841 ggtgattatg tccatgtctc cttataccaa ccagtagatg gcgaaaaaca atatgaagga
     9901 tttcttcaat catttgatgc tgaacagctt acattgaaaa tacgcataaa aacacgagaa
     9961 aaagaattga cttttgaccg aaaaaatatt gcaaaagctc gcttagctat ccaattttaa
    10021 tcacggagag gaatcagaaa aaaatgagta aagaaatgtt aaatgcgtta gacgctttag
    10081 aagctgaaaa agggatctca aaagaaatcg tgatcgatgc tttagaagca gcattggttt
    10141 ctgcatacaa acgtcactat ggccaagcac aaaacgtcga agtggaattt gatcagaaaa
    10201 aaggaaagat ccatgtctat gcggtaaaag aagtaacgga agaagtgatg gattcacagt
    10261 tggaagtctc attgaaagat gcacttttaa tcaatccagc atatgagatc ggtgatacga
    10321 tccgttttga agtaacacca aaagattttg gacggatcgc tgcgcaaacg gctaaacaag
    10381 ttattttaca gcgtgtacgt gaagcagaaa gaacgattat ttataacgaa ttcagtgctt
    10441 atgaaaaaga catcatgcaa ggaatcgtcg aacgccagga taaacgttat atttatgtca
    10501 atcttggtaa aattgaggca gttctttcta aacaagatca aatgccaaat gaattttatc
    10561 aaccacatga tcgaatcaaa gtttatgttt cccgagtaga aaatacgtct aaaggtccgc
    10621 aagtatttgt tagccgcagc catccagacc tattgcgtcg cttatttgaa caagaagtac
    10681 cagaagtcta tgatggaatc gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt
    10741 caaaagtggc agttcgctca aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc
    10801 caaagggaca acgagttcaa gcaatcgtca atgaattaaa aggcgaaaac atggatatcg
    10861 tcgaatggaa tgaagatcca gctgtattca tcgcaaatgc attgaatcct gcacaagtca
    10921 tggatgtgat cttcgatgag acaaatccaa aggcctgcac agttgtcgtt ccagactacc
    10981 aattatcgct agcaatcggt aaacgcggac aaaatgcacg tttagctgct aaattgacta
    11041 accataaaat cgatatcaaa tcagaatctg acatgacgga attttatgaa aatcaagaac
    11101 aacaaaaaga aacagaagaa ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg
    11161 atgaatatga aacaattgca ttcaacaatg aaacggtcga agaaaaacca gaagcttaac
    11221 tttagtaagg aggcaaagag atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt
    11281 caggtgaaat gaaacctaaa aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag
    11341 tatcgatcga tccaactgga aaaatgcctg gacgaggagc gtatgtctca cttgagccgg
    11401 aagaagtaca acaagcttgg gataaaaaaa ttctcgatcg agtactcgaa acaacattaa
    11461 cagatgagtt ttatcaagaa cttcttgact atgtcactca ccaaaaagca cgtaaagagt
    11521 tatttggaaa atgaatacag ttaatcggca aaaagccatg aatctcatcg gtctagcgat
    11581 gcgcgcggga aaactaatca cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa
    11641 agcaaaattg gtgtttgtgg caaccgatgc aagtgagaat acaagaaaaa agataaaaga
    11701 taagagttca tattatgaag ttccttgctt tgagctattt tctgaagcag aaatcacgca
    11761 gatgatcgga aaacctcgca aggtattcgg aataatggat gccggatttg cgaagaagac
    11821 caaggagcta attgaaggtt aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt
    11881 agcaaaagaa ctcaaaaaat ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat
    11941 cgatgtgaaa aaccatatgg gtgcgattac tagcagtgat gagaagaaat tgcaacaagc
    12001 gttcaaaaac ccaagcacga aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa
    12061 tcaacagccg aacgatcaac gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa
    12121 taaccgcagt tatcaagatc gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc
    12181 aacaacaaac caaaataata cgggaaatac cagcagccaa aatcgtcaag gaacgagcca
    12241 aggcggcaat caaaaccgta ctaacaataa caacaaacca aacaataaca atcgcggcaa
    12301 atttaataat aataaccgta atcgttataa taaaaaaggt aaaaaaggca aacagcaaca
    12361 atcaactaaa ccagctgtac caccacgcaa attccgtgaa ttgccagatg tattagagta
    12421 tactgaaggt atgaacgtag cagatattgc gaaaaaaatt catcgtgagc cagcagaaat
    12481 cattaaaaaa ttatttatgc ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga
    12541 tacgattgaa ctgttagcga cggactatgg catggaacca caagaaaaag tccaagttga
    12601 cattgctgat atcgataaat tcttcgaggc agaagaagtc gatccagata aattagtctc
    12661 acgtccgcca gtagtaacga ttatgggaca cgtagaccac gggaaaacaa ccttattgga
    12721 tacattgcgc cattcacgtg taacaagcgg cgaagccggc ggaatcacac agcatatcgg
    12781 tgcctaccag atcgatattg atggtaaacc aatcactttc ttggatacac caggacatgc
    12841 ggcctttaca agtatgcgtg cacgtggagc aagtatcaca gatatcacga tcttagttgt
    12901 tgctgcagat gatggcgtca tgccacaaac agttgaagca atcaaccatg ccaaagctgc
    12961 aggcgttcca atcatcgtcg ctgtaaataa aattgataaa ccaggtgcaa atcctcagca
    13021 tgttatgcaa gaattaagtg agtatgaatt aattcctgaa gcatggggtg gagaaacgat
    13081 ttttgttgag atctcagcga aattcggcca aaacatcgaa gaattgctgg aaatgatcct
    13141 gttagtcgca gaagtagaag atttaaaagc agatccaaca caacgcgcaa tcggaacagt
    13201 gatcgaagca cgtcttgata aaggtaaagg tcctgtgtca accttacttg tccaagaagg
    13261 aacacttcac gttggtgacc caatcgttgt cggtaatact tacggacgtg tccgtgtaat
    13321 gacaaacgat cttggccgtc gcgataaaga agcaggacct gcaacgccag tagaaatcac
    13381 tggcttgaat gatgtacctc aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac
    13441 agcacgtgca gcgggtgaag aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac
    13501 aagccgtgtg actttggaca acttatttga aagcctgaaa gaaggcgaat tgaaagaagt
    13561 taacgtaatc atcaaagcag atgtacaagg ttcagcagaa gcactggctg catcattgaa
    13621 gaagattgat gtggaaggtg tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa
    13681 tgaaagtgat gtaaccttag cagcagccag caacgctatt atcatcggtt tcaacgttcg
    13741 tcctacaccg caggcaaaag tgcaagctga tactgaagaa gtcgatattc gtcttcaccg
    13801 tatcatctat aaagccatcg aagaaatcga aactgcgatg aaagggatgc tagaccctga
    13861 atttgaagaa aaaatcaccg gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt
    13921 tggtacgatt gcaggtgcct atgtaacaga aggatatatt cgtagagata gcggtgtccg
    13981 tgtaatccgt gacggaatcg taatctatga aggccaactt gcaagtctga aacgattcaa
    14041 agatgacgtg aaagaagtca aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa
    14101 tgacatcaaa gtagatgacg taattgaagg atttatcatg gaagaagtca aaaatgacta
    14161 aaaactgata aaaggaggcc aatcattatg gctaactacc gtgatcgccg agtcggtcaa
    14221 gaaatcttaa aagaagtcaa tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag
    14281 aacgtaacga tcacagatgt gaaagttacg ggggacttac agcaagcaac gatctattac
    14341 agcattttat ctgacttagc atcagataag aaaaaagcac aagatggatt gaataaagca
    14401 tcaggattga ttcgtcgaga gttagggcac aatctaagta tttataagac acctgaattg
    14461 ttctttgaac ttgatgaatc agttgtctat gggaaccata tcgatgaatt gcttcgcgac
    14521 ttgaacaaag actaatcaaa aaatcccgca aatcctgtga aatcaggggt tgcgggattt
    14581 ttttgttttc attaagacgt attacctaag agttccatga ctttagtctg gtagcgtgtc
    14641 agctaaccaa aaaagagaaa aagaaaagta aaaaattcga ctttcttttt tctatagtat
    14701 ataaattttt gcatatagat aaagatcaag ataccgataa cggaagctag attgtaatct
    14761 attttagtga cagtcaggta tagccaagta actagaagat cagtggaacc tgcaccataa
    14821 aagtttgagt ttgtcggtcc gttgacagtt aaaagaaaaa tcacgttgaa gttattgata
    14881 tttccgatta cgccgcttcg atttggtcac gatccaaatt ttggataatc cctgtagaaa
    14941 ttagcatagt agctgggata ccaattcaca tattgacaaa gataacagag acttttacat
    15001 aaaacggatc aatcaggaaa ggaattgctc gatccagccc cagttttcca acaatgtgtt
    15061 aactggccta gcgtcattca gcaaattatg catgatgagg agcgaaatga actgcggaac
    15121 agccattgtg ataacgacaa gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa
    15181 atttcgtggt tttttatacc tgttcatttg gctagatata aaatccaaac aaatgaacag
    15241 aagtgcttat taggtttcaa ttgggagtat ctgcgctaga ataagagtaa taatgaaaag
    15301 gagtgttatg tatggattat tctagtaaag aatactttga taaaatgacg gcttggtggc
    15361 gtgctgctaa ttatctttct gttgggcaac tttatcttaa agataacccc ttactgagac
    15421 gtacactgaa accagaagat gtcaaaaaac atccaatcgg tcattgggga acgattcctg
    15481 gccaaaattt tatttatgtc catttgaacc gagtcatcaa taaatatgat ttgaacatgt
    15541 tttatatcga agggccagga cacggcggac aagtcatggt atcgaatgct tatttagatg
    15601 gaagttacac agagatctat ccagaagtaa cggaagatga gacaggaatg caaaaattat
    15661 tcaagcgatt ttcatttcct ggaggcattg cttctcatgc agcaccagaa acaccaggtt
    15721 ctatccatga aggcggagag cttggatact ctctttcaca tgcagtcgga gctgtattag
    15781 acaatcctga agtaatctct gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt
    15841 tagctggaag ctggttctca aatgtcttta tcaatccagt gatagatgga gctgttttac
    15901 cgatccttca tttaaatggt gcaaaaatag ccaacccaac gatcttggca cgtaaatcag
    15961 atggagaatt agcaaattac tttaacggac ttggctggga gcctttcttt attgaaggaa
    16021 atgatccaga aaaattaaat cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa
    16081 tcaaatccat ccaaaaagaa gctcgcctga agacagcaac agatgtcgtg atgccaaaat
    16141 ggccagtatt gatcgtacgc acaccaaaag gatggaccgg tccaaaagaa tgggacgggg
    16201 aaccaattga aggaaccttc cgagcacacc aagtaccgat tcctgtagat caagaacata
    16261 tggatcatgc agatgcttta ctccgttggc tgaaatccta tgaaccagaa aaattatttg
    16321 atgcacaagg acgtatttta gaagaaatca gagaaattgc gcctactggt gaccaaagaa
    16381 tggcaaaaaa tcctatcacc aatggtggaa tcgatccaag accgcttatt atgccagatt
    16441 ggaaaaaata tactttgcaa tttgaaaaac caggctccgt taaagcagaa gatatgaccg
    16501 aacttggtaa attcgttcgc gagatcattg agaaaaatcc agaaaatttc cgtatatttg
    16561 gaccagatga aacaaaatca aaccgattga atcaagtttt caaaacgaca aatcgccaat
    16621 ggatggaaaa aatcgaacca gaaaatgatg aatggctttc gccatctggt cgcgtcatcg
    16681 acagtcaatt atcggaacac caagatgaag ggttcttaga agggtacgtt ctgactggac
    16741 ggcatggttt ttttgcaagt tacgaatcat tcttgcgagt ggttgactct atgttgactc
    16801 agcacttcaa atggatacgg aaatcacatg acctttcttg gcgaaaagat tatccttcgc
    16861 tgaatctcat tgcaagctca acagtcttcc agcaagatca caatggatat agccaccaag
    16921 atccaggaat attgacacat ttggcagaaa agaaggcgga gttcatccgt gaatatctgc
    16981 ctgcggatgc aaatacattg ctcgcagtca tggataaagc attccgctcg tcagaaaaaa
    17041 tcaatttgat catctcttcc aaacatccgc gtgctcaatt ttactcagca gaagaagcag
    17101 ccgtcttagt gaatgaaggc ttgaaaatca ttgattgggc ttcgacagca aaagaggagg
    17161 aacctgaatt ggtcattgct gctgcaggaa cggaatctaa cttagaagca ttagcagctg
    17221 taactttgct gctggaagaa ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt
    17281 tattgaaatt acgtcatcca agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc
    17341 aatattttac aaaagacaaa ccgatcttat ttgctttcca cggttatgaa acattggtcc
    17401 gcacgatttt ctttgatcgt cataaccatc atttaatgat tcatggctac aaagaaaatg
    17461 gagatatcac gacaccattt gacatgcgtg tagtaaatga acttgatcgt tatcatttgg
    17521 caaaagacgc agctttaaaa atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg
    17581 atcagaaatt acaagaacat caaaactata ttcgtgaaaa tggtattgat ctgccagaag
    17641 tattggattg gaaatggaaa aatcttgacc aataaaatga taaaatcgat cccgcaaaac
    17701 agaatttttt acattgtatt atttgtcaaa ttaag
//
LOCUS       DAZUMO010000054        16991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000054 DAZUMO010000000
VERSION     DAZUMO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.054"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..662
                     /locus_tag="ACG8ER_001940"
     CDS             <1..662
                     /locus_tag="ACG8ER_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS5050514.1"
                     /translation="VSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQR
                     SIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIE
                     TALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSN
                     GILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSL
                     I"
     gene            complement(733..2043)
                     /locus_tag="ACG8ER_001941"
     CDS             complement(733..2043)
                     /locus_tag="ACG8ER_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS5050515.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2055..3473)
                     /locus_tag="ACG8ER_001942"
     CDS             complement(2055..3473)
                     /locus_tag="ACG8ER_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5050516.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3713..5281)
                     /locus_tag="ACG8ER_001943"
     CDS             complement(3713..5281)
                     /locus_tag="ACG8ER_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS5050517.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5285..5566)
                     /locus_tag="ACG8ER_001944"
     CDS             complement(5285..5566)
                     /locus_tag="ACG8ER_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5050518.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5563..6486)
                     /locus_tag="ACG8ER_001945"
     CDS             complement(5563..6486)
                     /locus_tag="ACG8ER_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS5050519.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6483..7361)
                     /locus_tag="ACG8ER_001946"
     CDS             complement(6483..7361)
                     /locus_tag="ACG8ER_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS5050520.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7348..8685)
                     /locus_tag="ACG8ER_001947"
     CDS             complement(7348..8685)
                     /locus_tag="ACG8ER_001947"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS5050521.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8688..9791)
                     /locus_tag="ACG8ER_001948"
     CDS             complement(8688..9791)
                     /locus_tag="ACG8ER_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5050522.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9865..11373)
                     /locus_tag="ACG8ER_001949"
     CDS             complement(9865..11373)
                     /locus_tag="ACG8ER_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5050523.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11339..12031)
                     /locus_tag="ACG8ER_001950"
     CDS             complement(11339..12031)
                     /locus_tag="ACG8ER_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5050524.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12053..13027)
                     /locus_tag="ACG8ER_001951"
     CDS             complement(12053..13027)
                     /locus_tag="ACG8ER_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050525.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13327..14199
                     /locus_tag="ACG8ER_001952"
     CDS             13327..14199
                     /locus_tag="ACG8ER_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5050526.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14781..16979)
                     /locus_tag="ACG8ER_001953"
     CDS             complement(14781..16979)
                     /locus_tag="ACG8ER_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050527.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         5024 a         3564 c         2769 g         5634 t
ORIGIN      
        1 ctgtttcatg cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg
       61 acacactacc gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac
      121 aacagtatca gcgctcaatc catgatcgac ctgataatta tcctgatttc aattctgagt
      181 ttggtcacct tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt
      241 tagttgaaag attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag
      301 atattgaaac tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta
      361 ttacgtttga ctgtggaaaa gaattttcta actggaaagc cattagtaac caacatgata
      421 ttgatatata ttttgcggac cctggaacac cttctcaacg cccattaaac gagaattcta
      481 acgggattct gcgtcgtaat ggactgccga aatcaatgga ttttagaaaa gtgaatcaga
      541 catttatttc cagtgtcagc aatcaacgta atcatattcc aagaaaatca ttgaattaca
      601 gaacaccaat cgaaatattt ttgagctatg tacaagaagc attttattct agcttaattt
      661 gacaaatcat atatcattaa aaaaaataaa ggtcaagcta aaatattatt taagcttgac
      721 cttattataa tttcaacgat tgtcaaaagt attgtttgca gctgtgttgc cagtttctat
      781 agataaatca cagcttttga aacataagtt ttgtactgct tcgattcgcc cgaattcttt
      841 tgcagttatt tggcaatgag aaaagcagac atcttctata ggcttttcag gaaatgctac
      901 taaatcaaaa gctcgggaag gaagtgagta acctggagaa aggcatgcct gtacgttttt
      961 gatgtgtatg tctttgactt tcgtcatttg ctttgattca ggtatttctt cagccactgc
     1021 ttgccaactt tctggcattt gagcaaggtc attgctattt tttctattgt attcattatg
     1081 ccagtccatt atccaagaaa aaggaaattg tacatcaatc atattcagat tttctaccct
     1141 gatattttcg atcactcccc ctctttcttt tgaagatttc attcgaatac cacagtctga
     1201 ctgaaaaaaa ttgatatcgt gaatatatac gtcactaact ccagcactaa cttcgctacc
     1261 aatagttaca ccgtatcctg aacggatttt acatctagca acctcgattc ttgatgattt
     1321 tcgattgact cttgcaccat tgccatctct gccagactta atagcaatac aatcatcacc
     1381 acaggccaaa tcacattcat agacacgcac atttgttgaa gaatcaatat caatcccatc
     1441 tgtacttggt ccatcattgt tgcggatgat tactttttct actaatacat cgttagaata
     1501 agtgatttgc aagttccaaa aacctgattt ctgaaaaatc aaatcacgaa caacaacatg
     1561 ttctgcatga tacagtaaac aagcgcgtgg acgtttaata gcataatccg caatccaacg
     1621 aagtcccttt tgatcgtatt cagctcttgt cccgcttttt tgatcttttc cccaatacag
     1681 ctcccaccaa tggtctcctt gaccgtcgat tattcctttt ccttctataa gaatatcttt
     1741 cgccgagaga acattcaaaa tcgctgctgg ccatttcatt tctacgccgg ctactcgtgt
     1801 atcgatttct ggaaatgctt cgatatctgt tctccccttg aggactgcac cttcttcaaa
     1861 gataagatga ctcttatctt ttaaaaaaag ggctccgact aaataaatgc cttttggaat
     1921 aaatacaggt aagtgttctt tcgcaccttg atcgattgct ttttgcagta ttattgtatc
     1981 atcttgttga gtgtcgtttg ttttaaagtt caaagcattg ataatccctt tttttgtccg
     2041 caatacgttt ctccctatct ttcattgatt tttttcaatt cttcaccaat aaatacagtt
     2101 tttgaacgat tcagtgggta acctactagc aaaatgattg cggatacaaa taagcagaat
     2161 gctggtaaca agtttgccat cgtataaatc ctttcttgta ctaaaactga ttggatggct
     2221 ccacctgttg tagacgattg gtaaccaatg agtgccagca taaatccgcc taccgctcct
     2281 gcacatgctt gtcctacttt tctcgcaaaa gaatttactc catatactgt tccatcttct
     2341 cttaagcctg tcttatattg atgataatca atcacgtcag taatgaatgc ccagaccatc
     2401 aaattgaata aaccagatcc taaagtagcg ataaataaaa gaatcagata tacccatgca
     2461 tttgttatat gaaggaaata catcagcaag tacatacatg aagaagcaag taacgcaaat
     2521 acgcttgctt cctttttacc aaataatttt gtcagctttg tagcaaatgg tgctaaaact
     2581 actactgttc cgtaagtaaa taaaagagca acagacattg cttgtttatt atggaagtaa
     2641 tcattgaata aataagtcat attcgtccca gctaaaatct gattgatcac aatgaaaatg
     2701 tcaacaatga ctaaaataat cagtgctcga ttagctaaca aaccttttac caactgagaa
     2761 gcagatactt tttcgttttt gacaactctt actctttcag tagtcaattt acaagttaac
     2821 atatatgcga caaatgctat tgcagcacat ccgattgaaa tccaaaagaa atgattccct
     2881 gaaacgactt gttgtccact cttgttatgg gtatacatta aaatcggaat gaagaatcct
     2941 gtagttgatc cgccaatcgc agaaccaatg cttcggaacg tggacaacga ggtgcggtca
     3001 ttaggatttg agctaattgc tgctgccatt gctccatacg ggatattgat tgttgagaga
     3061 aaaatcccat aaacaatgta tgaaacaaaa atataagcaa tcttcaccgt cattggaaga
     3121 tgttggacga atggcaaaaa caaaatgaca gtcactaagc aaaatggata cttcattttt
     3181 ctgatccaag gaataaatcg gccagtttct gttaactctg agacatcaca taatcttcca
     3241 accatcacat cagcaaacgc atccaaaatt ctcgaaatca agaataacgt acctacgatt
     3301 gcccctgaaa cgccaagaac attcgtataa taaatcatca agaaactatt gactaaacct
     3361 aaaataaaac agttacctag atcaccgaac atgtagccta tcttatcgga aatcccaaat
     3421 tccttttttt ctttttttac tgtatttgct gtaacagcat tttgtgaaag catttgaatt
     3481 cctcctacct cttctagtaa acgttttcaa aattataact ctttttttct tgtttttata
     3541 ttcacaatgt tggcttttta ttgcattatc ttgacagata aaaaacaaaa aataatctaa
     3601 aaacattgat gcataagatt gataactttt tttacataac aatatataca aacaaaaaaa
     3661 ttcactaact agtgaaaata agttagtgaa cctcatttat taaagaatta aattacagat
     3721 gatcttttcc atcatacttg aaaaactcaa aatcagcata acttcctctt tttttattta
     3781 gatcatgcac tgctatcccg ataaaatttc cagtaaaccc tcctgagagt atacacatgt
     3841 ttttttgcgg acctactttc ttccattctt cagttgatac gtctttcaaa tgaaactgtg
     3901 ctgtctcttt ctttacagtg actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc
     3961 aatcttctat ttgtgctact ccgttttctg attctatcat acgtaggcat tttcccttgt
     4021 cctcattcca agtaacataa caataaatat aattggattc atttaagtac agtaccagtc
     4081 cagccatctg attaaaatta ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa
     4141 aatccgtttg ccgaatagct aacaaatgat gttcgaaggt agattgaaga gattcaccgg
     4201 aaatcagacg taaaaaacct ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa
     4261 tgcgtctggc attccagttc ttctttagct ctccttcaaa tgtatccata aactgatgat
     4321 ctttttcttg ttgtacctct tcatttgtct caatcattat ttcttccatc ggagttgtct
     4381 ggctatcttt aagtcttaac caatcgtcgg acgtccaata cacttcttga atagcggttt
     4441 ctcttcccaa aatagccgca ccgtttaatg gtctggtaca aagatatgtc atgtaccatc
     4501 ttcctgacgg ggtttgtact aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt
     4561 tgtcactagc tgtcagcata ggaaaaccag gatcaagctc gtaaggacca gtaatattct
     4621 ttgctcggca aactgtaact gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat
     4681 aataataccc gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg
     4741 caaatatttt atagatggga ccatcaagct tcttcttctc tgcatcatat tcttgtaata
     4801 cgataccgct ggatttattc ggtgtgtcca gacgataatc ccatagttca ttgacaagcc
     4861 atttttttcc cgtttcaaga tcatggaaca aagaaggatc aaagccgtta ctatttaaat
     4921 aaattggttc ggaccaagga cctgtgatct cagatgcagt gatcaaataa ttgtggacat
     4981 ctttaaacgg acggcctgca cttttgacat ctgtgtaaac aagataaaac aatccatcag
     5041 cataactgat ctgtggcgcc catatacttc catttgtagg atttccttgc aaagatactt
     5101 gagtggtcaa aatatccgtt gtatgtgacc agttgactaa atttttcgac tcatatactc
     5161 ggattcctgg aagccattca aaagaggaga caataatgta ataccgctct ttcactctta
     5221 atatattagg atctggattg aatcctctta aaataggatt tttcactttg atagtctgca
     5281 tgattcaggc tccttttctc attcttactc gatataaact gaatgcccca gctgctaaaa
     5341 aagcaagtga aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa
     5401 atgtattttc taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa
     5461 actctgacgc agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata
     5521 gactgccacc gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg
     5581 cttttctaaa tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc
     5641 agagaatttg acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg
     5701 aacatgccca cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct
     5761 tcctaaatag acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata
     5821 ccctgttttt tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc
     5881 taagctatgg atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac
     5941 cgttccttca atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc
     6001 cggtgtacaa aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt
     6061 atcctgataa ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa
     6121 agcaacagcc tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc
     6181 taatacgaca ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct
     6241 accgtcaaga tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc
     6301 cacaagttta aaattggata atctgctttc tatatattca tcatatcttc cttcttttac
     6361 tatcaagacc tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat
     6421 agtttcaaaa tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat
     6481 cctcataaat gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata
     6541 tgaccgcctg ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt
     6601 ttgtccagcc cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt
     6661 ccaactaagc taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa
     6721 gctgtcaata gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc
     6781 aaaagtgttt cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc
     6841 aatgctgtca cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt
     6901 tgagtatctg tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata
     6961 cgcattcccc ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa
     7021 atacctccgc gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct
     7081 gtcagtactt ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt
     7141 ttcccgactg aaaaatctcc gccgtgagaa tgcaggtaaa ggaccgtcgg aaacggtcct
     7201 tctttttctg gataagcata gagggctggt actgtttcca attcatttaa gtacaattga
     7261 tagtattcaa gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta
     7321 tgtctgctag atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac
     7381 aaccagttca tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt
     7441 agtattttct aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga
     7501 aaccttaccc gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat
     7561 catagcagga acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt
     7621 gatttcttgt atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt
     7681 ccttgccgtg atattcgaaa aatggatcct tctgaatgct gatgtccgtt catctatcgg
     7741 ataagccttt ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag
     7801 attcagtata aatggacaca tcacattatc catgactata ttggatactc gaatatcttc
     7861 aacgatcccg cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa
     7921 gatacaattt gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac
     7981 accgccatgt ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct
     8041 ttcgtacgta tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg
     8101 gcaattacta atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga
     8161 atccgctggg ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt
     8221 gatcgtccaa cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt
     8281 atggaaactc atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt
     8341 atgccaccat ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc
     8401 tacattttga gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga
     8461 atgaacaaca ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa
     8521 atgaagttcg acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac
     8581 aactacggtc ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt
     8641 attcaattca tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc
     8701 acttcaatca atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt
     8761 tcactgatgt agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact
     8821 tgacagtttt tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt
     8881 aatccttgat gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa
     8941 cgtattcctt tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt
     9001 cctttccgtt ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat
     9061 ggaagtaatg cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct
     9121 tcatgaagta attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc
     9181 caaaaatgat gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc
     9241 catgcatgaa tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac
     9301 tgtctcaaaa catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa
     9361 aaaggagaac ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc
     9421 caaaaagctc cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct
     9481 gcttttttat atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta
     9541 tttacatggt cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc
     9601 acgaaatagt cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc
     9661 caaagtaatt ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac
     9721 cacttttctt catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac
     9781 cagttttcca ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag
     9841 aagtatttgt cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc
     9901 ttgtgtgatt cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc
     9961 ttcgtcaagc tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc
    10021 tgatcctact tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa
    10081 ttcttcattt tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa
    10141 agagaacgtc acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag
    10201 atccatatcg ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat
    10261 accgccagtc atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa
    10321 tttcttcaat tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat
    10381 accatttcct tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac
    10441 aggcactaat tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc
    10501 caccattcca gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt
    10561 ttgggtaact gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt
    10621 caaagctttc atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac
    10681 agcccagcca tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat
    10741 atcactaggc gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc
    10801 gttttctgta aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag
    10861 tcctagatta tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg
    10921 tatcggtacg ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat
    10981 ttgacttaaa tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt
    11041 taacgcattg cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc
    11101 tagttcatca gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca
    11161 gttgaatgtc agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg
    11221 cggtgaagca tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc
    11281 tgaagaactg ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact
    11341 aagacctaat tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa
    11401 tatttttgtt ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata
    11461 tagaatttgc tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa
    11521 cacctggcat tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta
    11581 atgagtaatg aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc
    11641 ctttccaatc ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga
    11701 acgcagcaat atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa
    11761 gtaaaacaat cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt
    11821 ctttaaaata tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt
    11881 ttctaatcac tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg
    11941 cgatgactaa accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc
    12001 ttccgtttat tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa
    12061 cgccgtcttc tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta
    12121 cgaccccttt aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag
    12181 ctgtataaat ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca
    12241 agtacatatg ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa
    12301 tgattactgg acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc
    12361 catccatttc tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga
    12421 tgatcgtaga ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc
    12481 attcagggga tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca
    12541 atacccctcc gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca
    12601 tgaagtgagg gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt
    12661 catttaatag taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga
    12721 acaagatcaa cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga
    12781 aatgtttgaa accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa
    12841 aagcaataac gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg
    12901 gcagaatcat cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc
    12961 ttctgagctt ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac
    13021 tttccataag cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata
    13081 actatctgaa ttacgtatat aatcattttt atcgtaagta aaaacgctta catagcaatg
    13141 atttttgtgc tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa
    13201 accttctatt tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc
    13261 accgatgata aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct
    13321 atctatatga aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg
    13381 tttctaattt tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct
    13441 tttgcttcta cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct
    13501 ttagacgcgt atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg
    13561 attggtgtta caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac
    13621 ggtctttcca gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct
    13681 atgttgttca gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc
    13741 aattcttact gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg
    13801 agaaactttt tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc
    13861 acagattttg gtgtattttt caaaattatg ctgccattag ctttaccgtc tattgctact
    13921 atctcgcttt tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc
    13981 aacgattcat ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt
    14041 gggatgcaag cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg
    14101 gctgttatcg ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat
    14161 tttgtcaaag gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg
    14221 gtagttgtgt cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc
    14281 ttaaattttt gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta
    14341 tcagaggttg tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg
    14401 cgacgagtaa cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca
    14461 caaaattatg ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga
    14521 actgtgacaa gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc
    14581 cataaataca aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga
    14641 aaaacagcct cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc
    14701 agcagcaaaa catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac
    14761 aagaaaaaca gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat
    14821 tccttctttt taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca
    14881 atctctttta cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga
    14941 ttttgaacaa aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag
    15001 ttagggttat aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct
    15061 gtgtagacga tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct
    15121 tgagagcatt ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg
    15181 ggatgataat tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa
    15241 tctactccca ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact
    15301 tcccgactca aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa
    15361 ttcttcactt cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc
    15421 ggatatctaa taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt
    15481 gaacttggac cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc
    15541 ttgctttcat tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag
    15601 tattctttta cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct
    15661 gcatcatctt tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc
    15721 agcattcgat tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc
    15781 aaatcacgct ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa
    15841 tagcagtcat tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt
    15901 tctactcgat ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta
    15961 actaagtgtg cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc
    16021 aaatctagtt ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga
    16081 gaaatcggac gggcaaatct gtccgaaaat aagtaggaaa aaagaccaat gagtcctacc
    16141 agtgttaaag acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca
    16201 ctagagtcaa cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac
    16261 gttttatctt tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat
    16321 acagttggtt gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt
    16381 tcaatagaaa gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct
    16441 atcgtagcaa ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt
    16501 tcgattcctt tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt
    16561 tgatactttt caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat
    16621 ttaccagcca aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa
    16681 tttagttctt tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg
    16741 aatatgtccg agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta
    16801 attttccatt caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa
    16861 ctcgattcga tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt
    16921 acggctccta ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt
    16981 ttcttcctct c
//
LOCUS       DAZUMO010000055        16845 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000055 DAZUMO010000000
VERSION     DAZUMO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.055"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..848)
                     /locus_tag="ACG8ER_001954"
     CDS             complement(39..848)
                     /locus_tag="ACG8ER_001954"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5050528.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1141..2577
                     /locus_tag="ACG8ER_001955"
     CDS             1141..2577
                     /locus_tag="ACG8ER_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5050529.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2747..3943
                     /locus_tag="ACG8ER_001956"
     CDS             2747..3943
                     /locus_tag="ACG8ER_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5050530.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4005..4190)
                     /locus_tag="ACG8ER_001957"
     CDS             complement(4005..4190)
                     /locus_tag="ACG8ER_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS5050531.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4421..7735
                     /gene="dnaE"
                     /locus_tag="ACG8ER_001958"
     CDS             4421..7735
                     /gene="dnaE"
                     /locus_tag="ACG8ER_001958"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS5050532.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            7903..8865
                     /gene="pfkA"
                     /locus_tag="ACG8ER_001959"
     CDS             7903..8865
                     /gene="pfkA"
                     /locus_tag="ACG8ER_001959"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5050533.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8939..10723
                     /gene="pyk"
                     /locus_tag="ACG8ER_001960"
     CDS             8939..10723
                     /gene="pyk"
                     /locus_tag="ACG8ER_001960"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS5050534.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10758..11660)
                     /locus_tag="ACG8ER_001961"
     CDS             complement(10758..11660)
                     /locus_tag="ACG8ER_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5050535.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11846..12415
                     /locus_tag="ACG8ER_001962"
     CDS             11846..12415
                     /locus_tag="ACG8ER_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS5050536.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12473..12652
                     /gene="rpmF"
                     /locus_tag="ACG8ER_001963"
     CDS             12473..12652
                     /gene="rpmF"
                     /locus_tag="ACG8ER_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5050537.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12822..14243
                     /gene="gndA"
                     /locus_tag="ACG8ER_001964"
     CDS             12822..14243
                     /gene="gndA"
                     /locus_tag="ACG8ER_001964"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS5050538.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14436..15122
                     /locus_tag="ACG8ER_001965"
     CDS             14436..15122
                     /locus_tag="ACG8ER_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5050539.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15119..16624
                     /locus_tag="ACG8ER_001966"
     CDS             15119..16624
                     /locus_tag="ACG8ER_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5050540.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5425 a         2946 c         3536 g         4938 t
ORIGIN      
        1 attgtcacaa cctctttttt gtggaatcta attctctatt atagtttaag tagtttttcc
       61 agataaacag ctaaaccgtc ttcctgattg ctcaaaggag tgacatcatt tgcaatgcct
      121 ttgagttggt cagtaccgtt agccatagct actccccaac ctgcataatc caacaattcc
      181 aaatcattat gctcgtcacc aaaggcaatg acgtcttttt gatcgataga caggtagtta
      241 gcaatctctt gcacgccttt tgctttttgg attccttttg aaacaatctc taagatggca
      301 gtaggtccac cccatgtccg aacatcgaca taactgccaa attgacgtgt taattcagca
      361 gatacagctc ctgcaaaacg tttgtctgtt cggacaagca aagatgtagg atttgtcgtc
      421 aaatttttag gagaaagcaa attcttctct cctggacgag aagaagcaaa aattttttca
      481 tcaaagaaat caaaactgtc aataaaaaaa gtatcacgat tttcagcagc gataaaatct
      541 aaatttagct gctttttttg cgataagatt tcaaaagcga tttcacgatt gatcaacgtt
      601 tctctttcac catcccagtg ctgattaggg atgtgtacta atgcgccgtt aaaattaacc
      661 atcggtgttg ttaattctaa ttgttggtaa aactgatggc tcattcggaa tgggcgtcct
      721 gtagcaatac tgacatgatg accggcattg attgcttttt ttagagtctc ttttgtttta
      781 gatgtaatca tcgaatcttg attgagggta gttccgtcca gatcgatggc aattaatttt
      841 ttattcaata agggtccttc cttcctcaac tagcatttgt tttagtctag catattttag
      901 gtaattaaca agagcaatca gctattccta acaaaaacta cagttatggc tatataaata
      961 gctaaacata agctaaacac gaacgttaag tattattttc gctttttttg ttaaaaaaac
     1021 gttgaaaagc gaatgaaacg tctatataat ttgaaattgt tcgatggatt tgccctattt
     1081 tacttatttt ttattattat agttttccat cagaaaaaaa ttcagggagt ggaacttttt
     1141 atggaaaagt ttttcggttt aaagaaaaac aatacaaatg tatcaactga aataatggcg
     1201 ggagtaacga cattctttgc catgagttat atcttattcg taaatccgac gattttgtca
     1261 gcatcaggta tgcccttcca agcagtattt ctagcgacaa tcattgcatc gattattggt
     1321 acgctggtaa tgggattgtt cgcaaatgta ccatatgctc aggcacctgg tatgggattg
     1381 aacgcatttt tcacttttac ggtcgttttt gggcttggtt attcttggca acaagccttg
     1441 gcaatggtgt ttatttgcgg attaatcaat atttttatta ctgtcacaaa tattcgaaaa
     1501 atgattattc gggcgattcc cgaaagtctt caacatgcga ttggtggcgg gatcggtatt
     1561 ttcgttgcgt atgtcggaat caaaaatgca ggattcttat ctttttcagc agaccagtca
     1621 gctatttcga gctctgtcgt tgaaggcgga aaagcgacaa atgttacgat aaatggtgga
     1681 atcgtccctg cgttagccaa ctttgacaac gctcctatct tattggctgt gattggtttg
     1741 gtactgacag ccattttagt tgtaaaaaat gtccgtgggg ctattcttat cggtatcgta
     1801 gcgaccacag tccttggtat ttttatgggt gttgttgatt taagttccat tgattggcaa
     1861 acgaattcgt taggtaattc atttaaagaa ttaggaacaa ctttcggtgc agcatttggt
     1921 tctgaaggca tgcaatcttt attcagtgat tcttccaaga ttcctcaagt attgatgacg
     1981 atcatcgcat tcagtttatc tgatactttc gatacgatcg gaacatttat cggtacagga
     2041 cgccgcacag gtattttctc aaaagaagac gagatcgcgt tggaagatgg tcgtgggttc
     2101 aaaacaaaaa tggataaagc actatttgcc gatgcgatcg ctacttctgt gggggctatc
     2161 tttggtacat cgaatacaac aacttatgtc gaatctgcag ctgggatcgg ggccggtgga
     2221 cgtactggtt tgacttcagt agtagtggca gtgctattcg cattaagtag tttattctct
     2281 ccactgatcg ccattgtacc aaatcaagca actgcgccag cattgatttt agttggagta
     2341 atgatgatgg cttcttttgc agacatcaaa tggctagata tggaagaagc tttaccggcc
     2401 ttctttgcat cgatctttat gggattgtgc tatagcattt cctatgggat tgcagcagga
     2461 tttattttct atacaattgt gaaagtaatc aaaggaaaat acaatcaagt ttctgttgct
     2521 ttgtggatcg ttgatatttt attcattatc aacttcatca ttctagcaac aatttaacta
     2581 aagagaaggg attggaacag tatttgttct gatctctttt tgttttacat aaaaagaacg
     2641 ttacatataa aatgaccttt gcgggatttt tctcttttta ctggcgagat cgttgcttgt
     2701 ttattcgaaa ataggtatga taaaagagac gaaagaggga tgatagatgg agattaattg
     2761 gagaaaaaat ttagtgattg cgtggattgg ctgttttttc actggtgcaa gtattagttt
     2821 agttatgcca ttcataccag tttatgttga gcaactagga acaccaaaag atcaaatcga
     2881 attgttttcg ggactagcaa tctctgtgac tgcttttgca tctgccgtgg ttgcaccaat
     2941 ctggggaaat ctagctgatc gaaaaggccg aaaactcatg atgatccgtg cagcagcagg
     3001 gatgacatta acaatgggat ctttggcttt tgtaccaaac gcgtattggc ttttgatcat
     3061 gcggttttgg aatgggattt tgtctggata tattccaaat gccacagcga tgattgcttc
     3121 acaggcacct agagaaaaga gcggatgggc gttaggaaca ttgtctaccg gagcgattgc
     3181 tggaaatctg atcggacctt caatgggcgg tgcactggct cagtggtttg gtatggaaaa
     3241 tgtattcttg atcacagggg ctttgttgat gatcacaacg gtattgacca ttttcttagt
     3301 caaagaagat ttccatccaa tcgagaaaaa agatctgata agcaccaaag aaattttttc
     3361 aaaaatggat catctctcta ttttgatcgg tctcttcatt acgaccttga ttttacagat
     3421 aggaatcact acgatcagcc ctattttgac cttatatatt cgtgaattga gcggaagtac
     3481 agaaaatatc ctttttgtca gcggcttgat tgtttcgata gctggtgttt ccgctgtctt
     3541 ttcttcaccg aaactaggaa aactagggga taagattggg aatcaaaaag ttctaattgg
     3601 aggactcatt ttatcatttt gctgttattt acctatggca ttcgtcacta caccgctcca
     3661 attaggtata cttcgcttta ttttaggctt ttcaactggc gctttgatgc cttctgtcaa
     3721 tacactgatc agtaagatta cgcctcaaga aggagtcagc cgaatctata gctacaatca
     3781 gatgtttagc aactttggac aagttttagg tcccatgcta ggatcgacag tcgcgcatgc
     3841 ttataattac agtgctgttt ttattgtgac aagtttgttt gttctgtcaa atattcttct
     3901 ttctttattc aactttcgta aagttcttcg cagcagatta taatggtggt gaggagtaga
     3961 aaataaaaaa gagcttgaga aatctcaagc tcttggctgt ttttttacga atgggcagag
     4021 gtttcttttc ttggcgcaac tgcaccaatc aaaaggataa tgacacccgc aatgaatgaa
     4081 atgattgttg tcaaagtaaa atcatatgtt ccgcttgtta atgcagcacc aatataaccg
     4141 actacttgac ctagaacgac agcccagaat aatgtaacga tatatcgcat aaagaacacc
     4201 tcttttctat cttaatttta ccagattttc tttgaatgta aagaaacgag accaaatctc
     4261 aataaaattt gagttcttgc gaaagaactc attcctacag attacaagca agcaaatgaa
     4321 gtggaataat caacttcagc gcctgctatc ttaatgaaag taaaaggatc aaacctttta
     4381 acgctagtga attttaacga aaaaaaaggc ggaatgcaag atgtttttcc cacaacttta
     4441 tacaattact tcttattctc ttttacaaag tacgatacgt attcgagaat atgtacaaga
     4501 agcaaaaaaa caaggctaca cttcattagc cataacggat aaagatgtct tatacggtgc
     4561 acctgaattc tatcgtattt gtgtagaaga aggagtacag ccaatcattg gattacatct
     4621 tagttatact tatgaagaaa aaaattatgg cttgcttgcg tacgcaaaag atgaaatagg
     4681 ttatcagcag ctgatgaaac tttccagccg aaaaatgatc caagaaaaat tggaacttgc
     4741 agaaatgaca gaaaccgacc atctagcgtt tgttgttccg gcaaatcatt ctgtttctga
     4801 ggctttcaat aaagaggata actttttcgg tcattggcag tttttacttg aaaatgtttt
     4861 tactagaaat ttttatatcg gcttgacaga aaacacgcct gatcaattgc ctgaatggta
     4921 tcagtatgtg ataaaagaaa attattcgct ttgtgcatta catcccatcg ctagtttaca
     4981 acaagaagaa ctttttgcac ttgaagtgat gaaacatatc aaagagggaa gccaaatacc
     5041 tttagaaaat ttatctgcat ctttaccact aacagcaaat gtctatcttc tttcagaaca
     5101 agaaatgatc aaacgattcg aaaaagcggg tctttcccaa gctgtaatag gcgcccagcg
     5161 tttagctgaa tcgatttcat ttgaattcca gttccatcag aaattacttc cgcattatcc
     5221 agttcctaat ggaaaacaag ctggagaata tttaagagag ttatgctatt cgcaagtgga
     5281 acgccgaatc actgactata caaaggaata ccaagaacgt ttggactacg agttatctgt
     5341 catccatcaa atgggattcg atgattattt cttgatcgta tgggatgtaa tggcttttgc
     5401 tcatagaaac aaaatcgtta ccggtgcagg tcgtggttct gctgcgggtt cattagtagc
     5461 ctatgtgtta gaaatcacag atgtggatcc attagaatat gatttgctgt tcgaacgctt
     5521 tttaaacccc gaacgcttta cgatgccaga tatcgatttg gatattcctg acaatcgacg
     5581 tgaagaagta ttgaattatg ttcgagaaaa gtatggacaa tatcatatgg cacaaatcgc
     5641 aacatttggc acaatggcag caaaaatggt tcttagagat gcagctagag tgtttggttt
     5701 atcgcaaagt gaagcaaacc gctggtcaaa agcaataccc aatcaattga aaatcacatt
     5761 gacccaagct tataaaactt cgcatcaatt agtagaatta gttcaggcaa gcgaaaaaaa
     5821 ccaactttta ttcaaaactg cagtgatttt agaaggattg ccaagacatg tttcgactca
     5881 cgcagcaggt gtagttatca gcgataagaa tctacttgaa ctagttccgc tccaacgcgg
     5941 ttctgatgaa gcttttctga cccagtatac gatgaatgag gtagaagcaa tcggtctttt
     6001 gaaaatggac tttttaggac tacgcaactt atcgattatt gattttactt tgaaagctgt
     6061 taagcgagta gaagggtatg agatccgatt aaaagagatc ccgctaaatg atgcaaaaac
     6121 gctgctcttg tttcaacgag gcgaaacctc aggagtattt caatttgaat ctgctggtat
     6181 ccggaacgtt ttacgaagat tagatccaga aaacatcgaa gatattgccg cagtcaatgc
     6241 attgtatcga ccaggcccta tgcaaaatat agataccttc attgccagga agaaaggaaa
     6301 agaagccatt cgctacccag atgattcact gatcccgatt ttgaaaaaca catatggagt
     6361 gatcgtctat caggaacaaa tcatgcagat cgcctcgact atggctggat ttacattagg
     6421 gcagtcagat attctgcgac gggcagtgag taaaaagaaa aaagacgtat tagatgaaga
     6481 aaagtcgcat tttgtatcag gagcattaca acagggatat tcggaaaaaa cagcagcgac
     6541 cgtatatgaa tatatcgaaa aatttgcaaa ctatggattc aatcggtcac atgcatttgc
     6601 ttattcattc gtgggattcc aaatggcgta tctaaaagcc catcatccag gtgctttctt
     6661 taccagcctg atgaattctg ttcggcataa cacatcaaaa ttgaaagaat atattgcaga
     6721 agcacgtaaa aataaactca aactttcctc gccgtccatt aatcaaagta attccagttt
     6781 tgagttggtc aataaggaag aaatcagatt cggactgaca gcgataaaag gtattcggcg
     6841 tgattttgta gaagatatat taaacgaaag aaaacaaaat ggcccgttcc agtcagtgga
     6901 tcaattcctc atacggatcg ataagcgttg gctgaaatta gagttactgc aatcactgac
     6961 agcagtagga gtttttgatg aattatctcc taacaggaaa caattgatgc ttgatctcga
     7021 aggaaaaatc caaaatatcg tttacagtgg tggaagtctt gacttgctgg gaatcatggc
     7081 attaaaagaa gaagaagtcg ctgactatac actagaagaa cgcttgaagc tggaagagga
     7141 gtacttaggt atttatttgt caggtcatcc tgcagaaggg tttgatcgtc ttcgtttagc
     7201 taaacaagtt cagctgatta cagatatcgt ccctaatcaa ccagtcaatt tattagtgat
     7261 gatcaaggaa gttagagaaa ttcggacaaa aaaaggagaa ctgatggcct ttgttgaagg
     7321 aaccgacagt agtggagaaa tttctgtcac agtttttcca actctttatc gtaaatatcg
     7381 taatttgttt gccgttgatc gagtgatctt tgttcaaggg cggagcgaaa tcagcaaata
     7441 taatcaggaa ctgcaactaa ttggagaaag ctttgctgat cctaaccagt tagaagccca
     7501 gtatccttca caaacttgct atttgcgtat tacagaagaa cgggaagatc ccgagataat
     7561 gaaacaatta caagtgattt ttcagaaaca cccaggatat ataccagtag ttatgtatca
     7621 cgaaaaaacg caacgaaaag tagttctgtc agatgcttat tgggtggatg acagcaagag
     7681 tataaagcgg caacttattt atctgcttgg cgaagataat gtggttttca aataacttac
     7741 ggtaaaaaat actacttgct ttcatttttt ttcatttttt tatccgtaaa tcaagacaag
     7801 tgagaaaaat aatgttagaa tgttagtcgt ggttaacgca aaaatggttg taaacctaag
     7861 tcatacttag gataaagtat tttaatgagg tgaatgttta ttatgaaacg catcggaatt
     7921 ttgaccagtg gcggagacgc acctggaatg aatgcagctg ttcgtgccgt tgttcgtaaa
     7981 ggtatttacg aaggactgga agtatacggg atcaattatg gttttgccgg tttagttgct
     8041 ggagatattc gtcgtcttga tgtggcagat gtaggtgaca agatccaacg tggtggtaca
     8101 ttcttgtatt ctgcacgtta tccagaattt gcaactgaag aaggtcaatt aaaaggaatc
     8161 gaacaattga aaaaattcgg aatcgaaggt ctagtggtta tcggaggaga cggctcttat
     8221 catggcgcaa tggctttgac aaaacgcggt ttcccagcag taggtattcc tggaacaatc
     8281 gataatgata ttcctgggac tgatttcaca atcggttttg atacagcaat caatacagta
     8341 ctagaatcaa ttgaccgtat tcgtgacaca gcgacttctc atgttcgtac attcgttatc
     8401 gaagtaatgg gacgcaatgc aggagacatc gctttatggt caggtgtggc tggcggagcg
     8461 gatgaaatca tcatcccaga acatgatttt gatatggctt cagttgctaa aaaaatccaa
     8521 gaaggccgcg atcgcgggaa gaaacattgc ttgatcatct tggctgaagg tgtaatgggc
     8581 ggaaatgaat tcgctgagaa actttcagaa tttggtgact atcatacacg tgtatctatc
     8641 ttgggacacg ttgtccgtgg tggagcacca agcgcacgcg accgcgtgtt ggcaagtaaa
     8701 ttcggtgctt acgctgtcga attgcttcaa gaaggaaaag gtggattatg tgtcggtatc
     8761 catgacaacg aaatcgttgc gtctgacatc attgatacac ttgaaaacaa caaacataaa
     8821 ccagacttat cactatatga tctaaataat tcactttcat tttaagagtg aaagaaacca
     8881 agctatttaa gctgtgtaca gctttaaata aacaaaattg aatgggagag ttttttaaat
     8941 gaaaaaaacc aaaatcgtta gtacattagg accagcaagt aactcagtag aaattatttc
     9001 tcaattgatt gaatctgggg caaatgtctt ccgctttaat ttttctcatg gtgaccatga
     9061 agaacaatta tcacgtatga ctatggtccg tgaagcagtt aaaaagacag gaaaagatgt
     9121 cggtattctt ttagatacaa aaggagctga aatccgtaca actgttcaag gcacaacaga
     9181 agctgatttt ggtcgtgcag gatacatcaa atttgaagta ggagatcaaa cacgcatctc
     9241 tatggatcct gaacatgtag gttcaaaaga aaaaatcgcg gttacttacc caggattatt
     9301 tgacgatgtt cacgttggcg gacacatttt atttgatgat ggtttgatcg atatgcaaat
     9361 cattgaaaaa gacgaagcaa accgtgaatt agtcgtagaa gttaaaaatg caggtatgct
     9421 aggatcacgt aaaggggtaa atgcaccagg cgtttctatt agtttgccag gtattactcc
     9481 aaaagatgct gacgatattc gttttggttt agacaacgat atcgacttta ttgctgcaag
     9541 ttttgtacgt aaagcacaag acgttctaga tatccgcgaa atcttagaag aaaaagacat
     9601 gactcatgtt caaatcttcc ctaaaatcga atctcaagaa ggtatcgaca acattgacga
     9661 aatcatcaaa gtttctgacg gtgttatgat tgcccgcgga gatatgggtg ttgaaatccc
     9721 agctgaacta gtaccaatgg ttcaaaaacg cattatcaaa aaatgtaatg ctgctggtat
     9781 cccagttatc actgctacac aaatgttaga atcaatgcaa gaaaacccac gtccaacacg
     9841 tgcggaagct tctgacgttg ctaacgctgt atttgatggc acagatgcaa caatgctatc
     9901 tggtgaatct gcaaacgggg actacccagt tgaagctgtt tcaacaatgg cacgtatcga
     9961 ccaagaagct gaaaatgcat tatcagaatt aggtacattc caattgaacg aattcgataa
    10021 aacagatgta actgaaacaa tcggtttatc tgtagctcgt gctgcaaaaa atctaggcgt
    10081 gaaaacaatc gttgctgcaa ctgaatcagg atacactgca aaaatgattt caaaatatcg
    10141 tccagatgca gatatcttgg ctgttacttt tgacgaacgc acaaaacgtg gtttgatgtt
    10201 aaactggggc gtttacccaa cagttgctga aaaaccatca acaactgatg aaatgtttga
    10261 actagcagct aaaaaagctg ttgaattagg tttcgcatca gaaggcgatt taatcttgat
    10321 cactgctggt gtaccagttg gtgaacgcgg aacaactaac gtgatgaaaa ttcaaatgat
    10381 tggttctaaa ttattagaag ctcaaggcgt tggacaaaaa tctgtcgttg caaatgcagt
    10441 tattgcttca tctgctgaag aagctgtaaa aaaagcaaaa gacggaatgg ttctagttgt
    10501 accatcaact gacaaagaat atatgcctgc aatcgaaaaa gcagctgctg ttatcgttga
    10561 agaaggcgga ttgacttctc acgctgcagt tgtaggaatt gcacaagata ttcctgtaat
    10621 cgttggtgct aaaggcgcaa caacaacaat tacagaaggt gaattgatta ccgttgattc
    10681 acgtcgcggc atcgtttacc gtggcgaaac aacagctatc taatttagct taataaaaaa
    10741 taaaaacagc tcttctttta cgaagagctg tttttttgtg ctgtaacgaa ttcatggata
    10801 acttggacta cttgttcttg aagaggcgtg acgatctgct gtgttcgttt tgccaatgaa
    10861 atcaagaagc gaggctgaac ttcatctgaa agagggaggc aaactaaatg atcttcagga
    10921 tggacagcaa tctctgccaa aaaaccgata cccacatttt ctcggaccat actctttaaa
    10981 atcgtcaagt cattgctttg ataaacaact tttggatgag tatgtgtttg tttagaaagc
    11041 ttagtgaatg ctgcggggtg gacataatgt tcgttcaata gtacaaactg ttcatctgct
    11101 atatctttga aagagacagt atcctgcttg gaaagaggat gtgtaggtgg cacgacgatc
    11161 atgaaacgtt tgtccaaaag taactctgtt tctaaatatt gatccacgat tggctgtgtt
    11221 gagcctaata cagctaaatc gatcctccct tgtcggatca tcccgtataa gtcccgagag
    11281 cctccatcgg taatctcaat ttgattcatc aattcatgct tgataaagta acttgacagt
    11341 ttaggaaagt attcattgcc gataatagga ggcatgccaa aacgaagctt tttcttttgc
    11401 aactgagtga tttcagctct tgcagtagaa atctcttgga gaatggtttg tgcatggaga
    11461 aacagctgct ttccagaagg cgtgacgatt aattcgtgat gcgatcgatc tcttgtaata
    11521 agtacggtgt caaactcttt ttcaagacgt tgtaatgcat atgtaatagt aggttggctc
    11581 acacggaaat aattagccac tttcgtaaaa tttttttgtt tgacgatttg gacatagtat
    11641 tcgatatctt tcgtgttcat gaagatcatc cgctccttta atggaataat gattattgta
    11701 aatgattctg aggatgatag caaacagagg ctttctaata aagatttttc tgttttttta
    11761 tgaactgcaa ggtgtcaaga aaatttcatt gacaacatgc tttttgccct gtataattgc
    11821 ttttgttgct taggagtgat gaaaaatgaa atggtcttta ttggaattga gaaaatacca
    11881 ggagacaccg ttaacttttc atgagacgct cgacttgaaa gccgctctca tgaagcgaga
    11941 caatttaata cttgatgttg cgccagtgga agtcgaaggt ttggtctcag ttgataagaa
    12001 aggctatatc gttcattaca ctgtgcagac gactttgaca gttccttcaa cacgttcact
    12061 agaacctgtt gaactgccca tggatttttc tgtagacgaa gtattcatga cagaagaaca
    12121 attccaaagt agagatgaac aaatgtctgc ggatgagatt ttgctaattg aaggtccatt
    12181 attgaattta gcagagtcga ttgaagacaa catcttattg gcgattccaa tgcgggtatt
    12241 gacaaaagaa gaggaagcag caacagaact tccaaaaggc aatgattggg aagttgtttc
    12301 cgaagaagaa taccagcgca gaaaaatgga agaagccgag aataaaattg atcctcgtct
    12361 tgcaaaacta tctgaacttt ttgattctaa atcaaaagac gacgataaca agtaagattg
    12421 gaatatattg tgtctaattt agatacaaga ctttacaagg aggtgtagca aaatggcagt
    12481 accagctaga agaacatcaa aagctaaaaa agcaaaacgt cgtacacact acaaattgac
    12541 aatcaaaggg ttaaacgaat gtccaaactg tggtgaaatg aaaaaaagcc atcacgtatg
    12601 tgcaaattgt ggttactatg atggtaagga cgtaatgtca aaagaagctt aatacatttt
    12661 aattagaaaa tgtaaaacct caaggctaac catggagcct tgaggtttat ttttttgtag
    12721 tcggtaaata aaaaatatgt tgttctttag tttgcaatgt agccgtgtta agctataatg
    12781 taagagatta gggagaaatc ccgtatgtag gaggagttca tatgtcaaaa caacaaatcg
    12841 gcgttgtcgg tatggccgtt atgggtaaaa atctagcctt gaacatagaa agccgcggct
    12901 attcagtggc tttattcaat cgtacagggg ctaaaacaac cgctgtagta gaagaacacc
    12961 cagataaaaa tttcaaagca acatatacaa tcgaagaatt tgtagaatct atcgagaaac
    13021 cacgtcggat cttgctaatg gtcaaagcag gaccggcaac agatgcaacg atccaagagt
    13081 tgcttcctca ccttgataaa ggcgatattt tgattgacgg aggaaataca ttctttaaag
    13141 atacgatgcg tcgtaatgaa gagttagcca actctggcat caactttatc ggtacaggcg
    13201 tttccggtgg tgaagaagga gctttaaaag gaccttcaat catgcctggt ggacaaaaag
    13261 aagcctatgc gctagttgca ccaatcttag agcaaatttc tgcaaaagca gaagacgaag
    13321 caccatgtgt cacatatatt ggcccaaatg gagccggaca ttatgtgaaa atggtacaca
    13381 acggtattga gtatggagat atgcaactga ttgctgaatc ttatgactta atgaagaata
    13441 ttttaaacct ttcagtagaa gaaatggctg atatctttaa agaatggaat caaggcgaat
    13501 tagacagcta tttgatagaa atcactgctg acattctgac acgtaaagac gatgaaggaa
    13561 caggtaaacc agtagtagat gtgatccttg atgcagctgg gaataaagga acaggtaaat
    13621 ggacaagcca aagcgcgctt gacttaggtg tgccacttcc attgatcact gaatctgtat
    13681 ttgcccgcta tatttctgct tataaagaag aacgcgtaca agcaagcaag attttatctc
    13741 gtacgaacga ttttgagttc acgggagaca aaaaagaatt ggtcgaaaaa atccgagaag
    13801 cgttgtattt cagtaaaatc atgagttatg cccaaggatt tgctcaatta cgtgtcgctt
    13861 caaaagaatt tgattgggac ttgccatttg gtgaaatcgc aaaaatctgg cgcgctggat
    13921 gtatcatccg agctcgattc ttgcaaaaaa ttacggatgc ctatgacaaa aatcctgaga
    13981 tcgaaaattt attgctagat gattacttcg ttgaaatcac gaaaaaatat cagcaatcag
    14041 tcagagacgt cgttgccttg gctgtccaag ctggcgtacc tgtaccgact ttctcatcag
    14101 ccatcgctta cttcgattca tacagagcag aacgtctgcc tgcaaatata atccaagccc
    14161 aacgcgatta ctttggcgca catacctatg aacgtgtcga caaagaagga atcttccatt
    14221 actcatggta ccatgaagaa taatagtctt tcggctgtta ggaaatgaga aaataaaaag
    14281 aggacgaaag agctgaagtc gattgttatg agtagacttc agctcttttt taaaattgct
    14341 atccatgata aaatgactat agatttttaa gcatattatc ggtataatga tagagttttt
    14401 aaaaagccag tagaatagaa aggataaaat aataaatgag caatattcta atcattgaag
    14461 atgaaaagaa cttggcacga tttgtcgagc tagagttgaa gcatgaaggg tataacgcag
    14521 aagtccacta caacggccga actggtttag atgcggcatt aaataatgag tgggatgcca
    14581 tattgttgga tctgatgctc ccagaattga acggtttaga agtctgcaga cgtgtacgcc
    14641 aagtcaaaaa tacaccaatc attatgatga ctgcaagaga ttctgtgatc gatcgtgtat
    14701 ctggacttga ccacggagca gatgactaca tcgtcaaacc atttgctatc gaagaattat
    14761 tagcacgtct tcgtgcattg ttgcgtcgaa tcgatattga aggcgataaa aatgtggcta
    14821 aacaaactac tattacttat cgcgatttga ctatcgaaaa agaaaaccgt gtcgtacgtc
    14881 gcggaaatga agtaattgaa ctaacaaaaa gagaatacga gttgctgctt actttaatgg
    14941 aaaacgtgaa cgtggtatta gcacgtgacg ttttactaaa taaagtgtgg ggatacgaaa
    15001 cagaagttga aacaaacgta gtggacgtat atattcgtta tcttcgcaac aaaattgacg
    15061 ttcccgggga ggaaagctat atccaaactg tacgcggaac aggttatgtg atgcgctcgt
    15121 gataaagccg aaaaaatcgg aaaaaagaga actgaaaggg ccctccttga caatcaagtg
    15181 ggcctttgcg agttctttct tcatattcgt tgtgtttaca attttcgctg tcattaccta
    15241 taaatcatct atcaatctga ttgtggcaaa agaaagaaac aatgtagagc gtacgatttc
    15301 agaagtagct tctcgtttgg ctaactcgga tgaagaactg acattagtaa atacgtatcg
    15361 taatctgacg aaagcgacaa atgacggaga tgaggtcgta gatcatactt cactggtcga
    15421 aggaaactta atgaagatcg attcctttat agctgagcta gggcagccat caatggatct
    15481 ttatgtttat gatttggacg agaacttgat ttttaaaacc catgaaaaca gtcgaaacct
    15541 gattcagact acacgtaaag ttccaacgat cgttacacta gaaggaaaaa ctggcttttt
    15601 atctatccag cctatatatt ctaaaggtac aagagaaaag atcggctatg cgcaaagctt
    15661 ctatgaatta tcttctttct atgatattcg aaataagcta ttattgactc ttattgtttt
    15721 agaagttgtt tctttgatct taagtagtat tttaggtttc tttttatctt cttatttctt
    15781 aaaaccactc aaggtactca gagatacaat ggatactata cgtaaagatc cgcaatcaga
    15841 tatccacatg cctgaaatcg acacaaatga tgaattggca gatttagctg aaatattcaa
    15901 tgagatgctt gaccgtatgc gtttgtatat tgaacaacaa gagcaattcg tggaagacgt
    15961 atctcatgag ttaaggacac ctgttgcaat tattgaaggg catcttagtc tgctaaatcg
    16021 ttgggggaaa gatgatcctg agatattaga agaatcatta agcgccagca tgcaagaaat
    16081 tagccgtatg aaaagcttag ttcaagagat gctagactta tcaagagcag aacaagtaga
    16141 tgttcattat gcgaatgaaa caactaaagg aaaagaagtc acctatcaag tttataataa
    16201 tttcaagata ttatatccag actatgtgat cactttagat gatgatttga caaaagaagt
    16261 gaccctgcaa atttatcgta atcactttga acaattaatc attatcatat tggataacgc
    16321 agtaaagtat tcaactacac gaaaagaagt ccatatctct atttcttcca ctctgaatga
    16381 atttgagatc gcgattcagg actttggcga aggaataccc aaagaagacc ttaataagat
    16441 cttcaatcgt ttttatcgag tggataaagc aagagctaga acgaagggtg gaaatggtct
    16501 ggggttgtct atcgctaaac aactggttga aaactataaa ggacgtatct tagcagaaag
    16561 tgtagtaggg caagggacaa tttttagaat ctttattcct atcgcaaagg cagaagacaa
    16621 gtaaagcatg tgctttactt gtttttttat atatccaaca aagaataagg tacaaaatac
    16681 aagcaagtta gaaattcagg aatgtcatta taaaaaaaac tagcgaacaa ttaaatagaa
    16741 taacatccgt taattaaacg taagtagctt ttagatgaac gaaaaaacaa tgaaatgctg
    16801 taatattcgt gtttaaaaat aataaataaa aatagggaat tttct
//
LOCUS       DAZUMO010000056        16795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000056 DAZUMO010000000
VERSION     DAZUMO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.056"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8ER_001967"
     CDS             complement(99..1229)
                     /locus_tag="ACG8ER_001967"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS5050541.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8ER_001968"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8ER_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5050542.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8ER_001969"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8ER_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5050543.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACG8ER_001970"
     CDS             complement(3485..4549)
                     /locus_tag="ACG8ER_001970"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5050544.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8ER_001971"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8ER_001971"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050545.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8ER_001972"
     CDS             4880..5692
                     /locus_tag="ACG8ER_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050546.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8ER_001973"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8ER_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050547.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACG8ER_001974"
     CDS             complement(6502..6732)
                     /locus_tag="ACG8ER_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050548.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACG8ER_001975"
     CDS             complement(6716..6913)
                     /locus_tag="ACG8ER_001975"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5050549.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACG8ER_001976"
     CDS             complement(7059..7937)
                     /locus_tag="ACG8ER_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5050550.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACG8ER_001977"
     CDS             complement(7976..9094)
                     /locus_tag="ACG8ER_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5050551.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACG8ER_001978"
     CDS             complement(9091..11190)
                     /locus_tag="ACG8ER_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS5050552.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACG8ER_001979"
     CDS             complement(11241..12062)
                     /locus_tag="ACG8ER_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5050553.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACG8ER_001980"
     CDS             complement(12059..12868)
                     /locus_tag="ACG8ER_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5050554.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACG8ER_001981"
     CDS             complement(12889..13383)
                     /locus_tag="ACG8ER_001981"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5050555.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACG8ER_001982"
     CDS             complement(13395..13895)
                     /locus_tag="ACG8ER_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5050556.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACG8ER_001983"
     CDS             complement(13960..16656)
                     /locus_tag="ACG8ER_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5050557.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         5108 a         3613 c         2700 g         5374 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attag
//
LOCUS       DAZUMO010000057        16585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000057 DAZUMO010000000
VERSION     DAZUMO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.057"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..1535)
                     /locus_tag="ACG8ER_001984"
     CDS             complement(108..1535)
                     /locus_tag="ACG8ER_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFS5050558.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            complement(1648..2436)
                     /locus_tag="ACG8ER_001985"
     CDS             complement(1648..2436)
                     /locus_tag="ACG8ER_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050559.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(2542..3342)
                     /locus_tag="ACG8ER_001986"
     CDS             complement(2542..3342)
                     /locus_tag="ACG8ER_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050560.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIEGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            complement(3681..4790)
                     /locus_tag="ACG8ER_001987"
     CDS             complement(3681..4790)
                     /locus_tag="ACG8ER_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5050561.1"
                     /translation="MKNKILLFLTLICTLVLTIYPASQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            complement(4780..5637)
                     /locus_tag="ACG8ER_001988"
     CDS             complement(4780..5637)
                     /locus_tag="ACG8ER_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5050562.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            complement(5712..7547)
                     /locus_tag="ACG8ER_001989"
     CDS             complement(5712..7547)
                     /locus_tag="ACG8ER_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5050563.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(7596..9986)
                     /locus_tag="ACG8ER_001990"
     CDS             complement(7596..9986)
                     /locus_tag="ACG8ER_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5050564.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKIKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(9976..10119)
                     /locus_tag="ACG8ER_001991"
     CDS             complement(9976..10119)
                     /locus_tag="ACG8ER_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050565.1"
                     /translation="MLSTKKVINRCGDFNLGMGAKVITTPTYRGSFSSPNWYKEGVTR
                     DEA"
     gene            complement(10256..11503)
                     /locus_tag="ACG8ER_001992"
     CDS             complement(10256..11503)
                     /locus_tag="ACG8ER_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5050566.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            11895..12311
                     /locus_tag="ACG8ER_001993"
     CDS             11895..12311
                     /locus_tag="ACG8ER_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050567.1"
                     /translation="MNKKRKLSVLITLISILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(12328..13065)
                     /locus_tag="ACG8ER_001994"
     CDS             complement(12328..13065)
                     /locus_tag="ACG8ER_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050568.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(13487..14050)
                     /locus_tag="ACG8ER_001995"
     CDS             complement(13487..14050)
                     /locus_tag="ACG8ER_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050569.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(14037..14564)
                     /locus_tag="ACG8ER_001996"
     CDS             complement(14037..14564)
                     /locus_tag="ACG8ER_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050570.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSDKEKIEYYKPFTFKLTNFGIKTSSSGLYEVLTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(14587..15972)
                     /locus_tag="ACG8ER_001997"
     CDS             complement(14587..15972)
                     /locus_tag="ACG8ER_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS5050571.1"
                     /translation="MKKSKKTFGTLLIAVMQIIGITASLITINPQVTHADGIATYDAQ
                     FNCSTEGSQGSSGSGKDSDGNETGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGIL
                     GNWKQESGNDPRTVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKN
                     FGGAWWTTEAQLDFMFKGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAV
                     VAVRGGNAKEIWEYMKSKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACA
                     TTDDANSGSGSMGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFN
                     GIYQGQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDG
                     WLWAVGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFS
                     GVGKAKFEIEK"
     gene            16055..16546
                     /locus_tag="ACG8ER_001998"
     CDS             16055..16546
                     /locus_tag="ACG8ER_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050572.1"
                     /translation="MTDKTGNLPKLAPKTSQTKSKKDEKIKNAVKKKSVKESSSLLLD
                     IPMSTFDEILDDNTDETSVKIASHVGMNSQNDSFYHLLNSLRELEDKKNQVIASKAPD
                     LTTDLDAEIIQVKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISEQKK
                     SDD"
BASE COUNT         4745 a         3322 c         2444 g         6074 t
ORIGIN      
        1 aaggatggca aaaaattatc aagttaaaaa aataaatttt gaaccaaatt atttttaaaa
       61 aaatagctag tttttttatc aaaaaaaata gagcttttca gctctattca gactgtagac
      121 aaaaaggagt ggcttaattg ccaactcctt tttgttagtc tttgtagaaa ttttaaataa
      181 aaagccatag gcttttttgc tttccatagc catgtggcta gtttttttaa attcatggca
      241 gcaaacgtaa gcatcgtgtg cgtggcgact ttttcgagac ctcgatattt cgtccaacgc
      301 ataccatgct tttcttttgc atctgcaaat attcgttcaa tggtttgttt tctcttttta
      361 taaatttctc tattcaacgc cgtatggcgt tggtgttcaa gttcatccat aagatccgcc
      421 caaacatgtc gagttactat tttttgatgt tttgtactca aagtacagtt tttaagtaat
      481 ggacaactcc tacagaattt tggatttgat ttataggttc gataaccatc acgagtgata
      541 gttgaaaata acaacggttg attattggga cataaatagc agtcaaaata ggcatcataa
      601 acaaagtctt ttttacgaaa cattcctttt actccatgag gtacagtata tggaagaatc
      661 gggtagagtt tcaagtgtga cagaaaatga acaaatttag gcgtcttata tcctgcatca
      721 gcgacaacac gctttatatt tggaaaagct cttttcgact gtttgaccag atcgattgcg
      781 acttggctgt catgtacatt tcctggtgtg attaatgtag ataagataaa accattatct
      841 tcacaggaag tatgcgcgga ataagcaaat tgtttttctc gttcaccttt aacatagtaa
      901 ccactttctg gatctgaatg acttattttt tgaagactca actcactttc tgtgtcccat
      961 gtaaaaggac gttttcctac agcaatacgt tctttattta tttcatcttc taattcatct
     1021 tgataagctt tagcttgacc gtaggtcatt tcttttgtga acttatgttt attggcattt
     1081 gccttaatat gagtagaatc aatataaagg ttgtcttccg taacgaagcc agcttttaca
     1141 gcttgttcta gaatatgaga gaaaatgtct tcaaataaag ttgtttctct gaatcttcga
     1201 acatagtttt taccaaaagt agagaaatgc gggataggat cctcgaatga ataacctaaa
     1261 aaccaacgat aagcaacatt tgtttcaacg tctttaatag tctgtctcat cgaacggata
     1321 ccaaataaat attggataaa tactaattta attaagacca ctggatcaat actgggacga
     1381 ccatgggtcg aatacgtaga ttctacaatg ggataaatga aatcaaactg aatcgcagca
     1441 tcaatctttc gaactaagtg atctttaggt acaagatcat caattgttaa catagcaaat
     1501 tgatttctac cttcaatcga ttgttttctc atcattagaa tcactcccag aatagtttat
     1561 acaatgagta taaaacaaaa aaactgcaaa caacactccc aatttaggag tttgtctaca
     1621 gtctgaatag agcttttcag ctctatttta ttcaatatca ttttgatcaa ttaataatcg
     1681 tacactccaa ttaaccgagt tttgatcact caaatattta taatctttgt catcaaaaac
     1741 cagttgccaa actctggtac gaacagtttc ttcgtccact tcctcatctg aagagtattg
     1801 cgataatgct tcttcaacta tatactttac aacttcctca aaatcagtag gaactttatc
     1861 aataaccact tcatagtctt cttgttttac gatattggga tcaatatgtt catccaaaca
     1921 tacctcagtg agtttttctt tcacctctgt atctctttct gtttttaggt actctgcata
     1981 gtcttcctta gaccattgtt tttcaagatc atccgctata cttttaataa aggattcttt
     2041 atttaaccaa ttatctgcgg tatctatacc ttcaaactca aaagctgttc cttctatacg
     2101 tgatttgacg aaagcatcaa ttttcttgta ttgattattc atttcccatg aaacacactc
     2161 atcttcttgc gtaatatcat aaattttttc ggaaaaacta actaattcat tccacgattt
     2221 ctcctcggca aattgactta gtttcgatgc gataaaagga aattttgctt ttaagtcatc
     2281 catattcttg atctcaaatt cgatattttc ttctcctaca gctgaaatcc atgctgaaag
     2341 attgattgca atatcgctaa agtcttctac attttcgaca tttcctgtat tatcttttat
     2401 tactgcttct aaagccatat cggttgttgc actcatggtt tgatctcctc tcataataga
     2461 gcaaaaagcc ggttaaaccg gctttttgca tggtacgttt tgtttggtag ttttctgctt
     2521 gtgtttatgt tgaaataatc cctatagctc ttcttttaat agttcttcaa ctttatctct
     2581 taactctccc tgaccactct ctaatagatt taaatagctc atcagaatat ccgtttcata
     2641 cgtttttaat tcagctctat actcactttc tgaatatgat cgtaaatacc catcattgct
     2701 aaatgcaact agatcatctt gaaagttcca ttctcctaaa tttgttttac tcactgcttc
     2761 ccatggacta ttactaaaca tttcattaaa gaaatcttcg tcgttttgat ataccttcaa
     2821 ttcaggaaaa tagttgctgt cttcattgat ttcttgcacc atttttccaa gttcttcttc
     2881 agataattta ccttccaata attttacttt ttctctgtca tctgcataag ccatttgaac
     2941 ctctcctttt tctttttcaa tttcttcagg tgtttgtttt ggcgtaactt tcaaatctat
     3001 tccattgcct attagaccca ttagataacc atagaacgtt ttaaagtcac ttgtagcaag
     3061 agatttggca tcagggtatg tatccccttc aattttatct aaaacaaaat cagcaatcgt
     3121 tacccaagga gaatatatcg tacacactac atcccatgta gcatcttcaa tattcgtcca
     3181 tgtcggaaag tcattttcat tcaaatagtc ttttttcagt gtttcgatca gagtttttcc
     3241 actttcgttt tggcttagat attcaacaaa ttctgcacca tcaaacctgc tactaaatag
     3301 tttagctaat gagtctaaag ctgtttttga tatttccacc attactacac ctcctattaa
     3361 tattttacga tgacttgttt ttcattcaca aataagattt tctacatctt atctattgct
     3421 ttatagcttt tccatacatt atttctaaat acaaccaata accctattaa attaagcttt
     3481 tgagtttatc ttttctaata atattctgat aaatattctg ataaaaaaat ttgttgtatt
     3541 tttttcttga ctttttcttt ggaattaaga tcagttttga ttttatttaa aagtgaatct
     3601 agctctattc aaaataaaaa agagatcaat aatagtatca atcccttttt actgtactct
     3661 atcaattttt ataatcttca ttatgctttt ctaaactctt tgattaaaag aataagaacc
     3721 tttagacttg ctaatgctcc taatacagtc caaatgacta aaagcatttt ctgatgttta
     3781 ttggtaattc ctgtaaatat tgaacttgcc ttttcagcaa acgttctgtc ttgcatctct
     3841 acgctacctt gttccggaat attaagatca ttatttccat ctaattcctt gttggcacga
     3901 tcaacatcct caaataaatc attagaagat gtaacagttt ctggcttatc tgaaacggat
     3961 tcactatcaa tggcatcagc gtatgcggta ttgccaactg ttacttgtgg cgtgatgtat
     4021 tttgcttttt catctcttag acttctaact aataaacgat cgtagttctg aggatatgga
     4081 tgacaagtat aaagtgttaa catatctaaa tcaggaatat cagtcagtgt tgaattctga
     4141 ccgtaatttc taataacgtc tgttcggaaa accgtataaa ctgctttttc accaagataa
     4201 tctatataaa tgtggtctcc ggctactaat tgatcaacat aaagaaattg tggtgacgta
     4261 taaccgccac gatgacctgc tattactgtt tgatgacctt tacctgataa aggtagacta
     4321 gtagcactca tttgtgccac acctctatat aaatgctcct gtgaagcgcc tagaaacaaa
     4381 agctctagca catttatttt tggaatatag acataaccaa ccgctcctga ataggtagat
     4441 ggttcaaaat ctttcgtttg attttttgaa aaaacatctc ctgtgtatgg attgaccttt
     4501 ccttcagctt cacgaatatc aaatgatcca ccgtctgttt cctgagtatt gtctactcct
     4561 ttatctgttt tctgagtatt gtctaatcct tggttaccgc tgttcgattt tatttgttcg
     4621 actttttttg ccacttcctc ttttgaagga gtttgttttt tctcaatttt ctcttggatt
     4681 tccgccacat tctcttttgt tttgaattga tgatagagct gacttgcagg ataaattgtt
     4741 aaaaccaaag tgcaaataag agtcaaaaaa agtagtattt tattcttcat ttttcattcc
     4801 tcgtctcttt gctactattt ttttaataat gactatcaca gctattatta ataaaacccc
     4861 tacaactacg ataggatacc agttcatctg cgcctttacc tttaaatctt ctttcttctt
     4921 ttctttctca acaatacttt tttggtaagg tatgctaacc cctctaacga gtaacctatg
     4981 agaattatct ccaacaggtg tacatgtcat aagtgttaca taatcttttc ctgccacagg
     5041 aagcaaatcc tcaaattcgg ttggtagaat tgtttttatc cgatctactt gataagcaag
     5101 ttttttccct gaaatatcaa taaaaaattt atcaccaagt ttgagcaact ctaaatctgt
     5161 aaaatatttt ttattaggaa tgcctgaatg agctgatata acagaatggt ttccaatccc
     5221 accagttgga taatcagttc cggttaatat tcctgctcct gctgatagtt ggaaatcgtt
     5281 tacaccatca taaattggca aatccacatt aatctttggt ataagaattt cacccactaa
     5341 ttcacctaat ttatccttct gaagatttcc atttgacgga atattgttat tatcttcagg
     5401 aatcgtgact gaggtttgtt ctgtgtcttt tcgtttccta ttaacttctg aatatctctt
     5461 gaagacttct tcattctcct tttcagaagc ttcatcatat ttttccttat aggagtttag
     5521 tgtttgttga gtttggtagt aggtataagc ctgagtaatt acaggatata gacctactcc
     5581 taaacatagc aacatcccaa tttttaagat gatcaacttg atattcttct ttttcatgct
     5641 catacctact tttcacacac tttttaaaaa gaaaccctag agccaaaaac actctaggga
     5701 attttatttc attaagcttt gtgttgttct cgtttgtaga caacatatag ccctgttcct
     5761 gccacacctg caaccactag aaatactact gttccaattc cacctgtacg aactgtttca
     5821 ggtgttttaa ggttcttgat gtctgttact tcgctagaag catcatcatt cgataccaca
     5881 aatggaacat cgtttctagt tggtgtatat cctgaaggag caacaacttc ttcaagagca
     5941 taatcattga tttttttatc tgtgattgtt ggattacctt gtttatcaaa atctacatca
     6001 taggtatatt gcaaaccttc aatttggaac ttaccgtctg atccggaaac gaattcagta
     6061 gctttagatt tatctgcaat ccaagtaaca gctttcgttg ttggatcttg ttttaggtaa
     6121 gtaactttat tcggatcaat tttgttcttc actacaaatt ttgctccact caatgctttg
     6181 cttgtatctc catctgtttt aatgaattgg tgatcaccaa tgattgtata agcgtgatca
     6241 actggtgttt cgtcttcggg ttcatatcca ttgttgtaga tcgttgaaac atcattctta
     6301 agtggtgtat ccattttagc tttactatta gtatgagcgt tcatcaaaat tgtaaagaag
     6361 cgatcccctg catcaaattg tttctgaagc ttatccatac ctgattcagt aaagacatag
     6421 tttgtcgttt gtccgtcacg ttcgatactg taatcagtat ctaaagtcat tccttcgatc
     6481 acttctgatg aatcataagt taactctgca cctagaacat ctttgacaac atatttttcg
     6541 taattaccaa tattaccagg aattgatggt ttcaatgtat attgaacatc ttcgccagta
     6601 tcaacggttt ctttatcaat taccttagta attacaggtc gtttgtagtt ggtaacatca
     6661 agaactactt tagcagtctc cccacgttta acgctaaatt tgaatttttt accatctagt
     6721 aaataggttg ttctattagc atctgttccc aaatcggttt ctaggaaata atagtcacct
     6781 aattccaaac catccacagt taatttacct gtgctgtctg ttttataagt tccaacaacc
     6841 tgatcagatc catcatcttt tcgcaattca aaaacagttc cggcaagagc ctttccatct
     6901 tcgtcttttt tatcaagctc tgctgagttt aattggaaat agtttttagg atagacatgt
     6961 acatcataat taaagtattg atcatctgat ccgtttagtt caggtacgtt aacaatcata
     7021 ggatacgctt cttcaactac acctttgact gttcctgttt caacaatcaa atacaagtta
     7081 tctgttaaat cgtctgtatt tacttgtata ggtatgtcta tattcccttg tgaatcagtc
     7141 ttcattctac ttacttcact gtattttttg tttagtaata atcgtgcttt agcatcatca
     7201 atatgttgac cttttgtagt atcactatcc gtccatccca aggatttagc ttcatcttta
     7261 gtaagtttca cataagagaa ctcaacgcct tcaagaggtt ttacaccatt tggtaaagct
     7321 atttctttcc ctttgttatc aatatctgag acagggttcc catccccatt agtatttgtt
     7381 actttatgga tatgaatcgt tccagttcct tgataattat agttttgttg tgcatatgct
     7441 acttgccccc ctagtccaag gctagaagag attgagcttg cgcccaatcc cactcctagc
     7501 aaagtaccaa atagcattaa atttgaaaaa attttttttg atttcatacg tcagccttct
     7561 tttttcatat ttacttcttt tccattaaca cttttttatt ttgctttgtg ctgttctcga
     7621 taatacagta gcaccatact caatcctgca attagaaata caagaaggaa ggctgatgta
     7681 ccgattccgc ctgttcttac tgtttcaggt acaaggtgat taattactgt atattcaaag
     7741 gtttgtccat cgtcagcaac ggtgatgcct tctttagacc atttccctgt ataaccatta
     7801 gggttttgtg tttcttcgat tttgtacgtt ttgttggcaa caatattaaa tgttgccaag
     7861 ccatttctgt ccgttttttg tacaactttt acacctttgt cgtccgtcaa agtgaattct
     7921 gcgtttgcta atgctttccc ttgatcatcg actttatgaa ctttgatcac accttttttc
     7981 aaagtgtttt tagctgtata ttcaaagact tgtccatcat ctttgacggt aattccttct
     8041 tgtttgaatg cgcctgtgta acctgttggt acttttgttt cttctaagtt gtaagttttg
     8101 ttcgcaataa tgttgaacgt agccagacca gttgcatctg tctttttaac ttcttttaca
     8161 cctttttcat ccgttaaggt gaattctgca tctgctaaag gttttccatc ttgatcgact
     8221 ttatgaactt ttactacacc ttttttcaaa ttatttgtag ctgtatattc aaaagtttgc
     8281 ccatcgtctt taacactgat accctcttgc ttaaatgcac ctgtgtaacc tactggtact
     8341 ccggtttctt ctagactata cgtcttgttt gctacaatat tgaagcttgc aagtccattt
     8401 tcatctgttt tttctacttg tgttgcacct ttttcatcgg ttaaagtgaa ctctgcattt
     8461 gccaaaggtt ttccatcttg atcgacttta tgaactttta ctacaccttt tttcaaagtg
     8521 tttgtagctg tatattcaaa agtttgtcca tcatctttaa cggtaattcc ttcttgcttg
     8581 aatgcgcctg tataaccagt tggtactccg gtctcttcaa ggctgtaggt cttgtttgct
     8641 acaatatcga agcttgcaag tccgttttca tctgtttttt ctgcttgtgt ttgggatgaa
     8701 tcatccgtca aggtaaattc agcattagct aatggttttc catcttggtc aaccttttta
     8761 acttttattt tacctttttt aattgtgttc gtagcagtgt aactaaattc ttgtccatcg
     8821 gtattaacag taataccatc ctttttaaat gaaccttgat aaccttcagg aacttttgtt
     8881 tcttctaagc tgtatgttaa gccttgttgc aaatcaaacg tagctaaccc atcatctccg
     8941 gttgtcttaa cagatttagc attgttattg tcagttagag tgaactccgc tccttttaaa
     9001 gcatttccat cttgatcaac tttcttaact gttacctgac caacagctct tgtccattta
     9061 gcactcaaag atttagagtc ctttgtttct gaagtagtga tcataactac ggcatcttga
     9121 tacacacctc gtccatcaaa agtaaaagga ttggtcgtgc taacattagc agtaccttta
     9181 aatgacaaag taccacttgg tgtagtcaca ggtactttaa tagtaaaact tgaatttaat
     9241 tttacagtgt tgttagtaat ttctttacca tctgcatcat aaatcttcat accagcaggt
     9301 tttgctgatg taaaagtgag cttcgcatta ccagtcttat ttgatgctgt tttatatgtg
     9361 aaagtgtgat attttccttc gtcagctgtc ttagaagctg tgatagaaaa ctcagtgttg
     9421 ggcgtatctt ttccattctt agcatagtca taaatttttt caaatgcttt ttttacacga
     9481 gctgtttctg cggcggtatg gtttggatgt gaccatacaa tctgtgaaac tttaaagttt
     9541 ccggctaaaa cccatgaaac taattgtgtt gcataccatg cttctgtatc atttcctgca
     9601 cccagttcct ttgctgtttt tgatggaaaa ccacctttaa acatacgata gaactcatca
     9661 ctgccagctc ccttgtactt caagcttccg gctccacttc ctgttgggga tggcaattct
     9721 gaatccatac aatatccaat atttccatga gaatctttaa attgacttga aatcatccat
     9781 tcatttggat gattcggcat attaacccta atataaccgt ctgtttttac gccagtttct
     9841 gtaaattttg cacttgtcgg ttttgtgaca tctgcttcag ctcttattgc tgaaaaaggc
     9901 attataagcg tagataacac cattactaaa gtcgcaaaac aataaattat ttttgatttt
     9961 aaaattatcg ttttttcatg cttcatctct cgttactcct tctttatacc agttaggcga
    10021 agaaaatgag ccacgatatg tcggtgtggt aatgaccttc gcgcccattc ctaagttaaa
    10081 atcaccacac ctattaatca cttttttcgt tgaaagcaag atgtttcaac gctctattat
    10141 tcatagcatc acccctttta gttctaccca ccatgagcaa caagttttct aagtagttaa
    10201 gtaagggcaa ataaagacta tacttaacta cttagtatat aaacgatctc ttttattaac
    10261 gtaccgaaac cgctaattga gatacgttat atgctgtttc aaatgatcct gcattaatcc
    10321 aacttgcccc aaacacagga tcgacaacat ggtaaaaacc gtctttaaat ccatcaacgg
    10381 ctactacatg tcgattccaa atagcatcgc cccaaaacat tgatttatgt tgagcaggtt
    10441 caaaggcata agttacccat gctactacag gatttccatt tacaatctca tcttttaatc
    10501 cttgtaccga tgaaccagaa atatctacaa catttccgcc attagcaaca ccccatgaaa
    10561 ctacaggagc cggattcata ccttgataag ttccatccac attgtgtctc cattcaccag
    10621 aaaaaccatt atttggatta ttatcaggag atagtggcat tgatttaaca aaagtcatta
    10681 aatcttgatt tgttgcaaca cctttataat gtaacgcttc tagcaaagat gttccttcgc
    10741 aaagcattgt gttaccttga ctaataaatg gaacgcctag cacaacatta gttggttgtt
    10801 cgggtttact tggtgaagta gaactgtcag tttcatcact cgaacctgtt tgtgattgat
    10861 cactagaaga tgtactggtt gattcatttg catcatttga tgttgattgt gagttatttg
    10921 ttgatgttcc agtatcagct tccgtagtgc tactttcact tgttccctgt gatgcttctt
    10981 tctcacttgt agaattattt gtattagatg aacttgctcc atcaacactt gaagcacttg
    11041 tttctgtctt atcagaagtg tttgctgttg gttccgattc ttttgtagtc tcggattttt
    11101 tgttctcttg ctccgtattt tcaacaggat caggtttatc cttttgaaca gtttgactaa
    11161 ctacttcgcc atctgcttcc ttaactgtta atgtatcttt actgatgttg aaagttcgtc
    11221 caattgggat agtccgttgt tctccttttg ccaaatcaac attgttcaat tcggcaaact
    11281 cagcaacagt agtcttgtaa cctaagcttt taagcttctc tacagttgac caaatagtat
    11341 ctcctttttt gaacgcataa cttgattgtc ctgttgtaat ttggatttga tccggtgtgt
    11401 ttgcagtcca ttcatcagca tgtgaggtta ctggtactga taaccccgca agtacagctg
    11461 atgcaaataa aaccatacca agcgaatttt tcttcatatt cattccttct ttttgttttt
    11521 attttctgtt tgtgatcgca atgcttctat tccttcttcc gttataatat aggttttgtc
    11581 acccactttc ttgaacgaac ctttaggcaa acgatttgga taatagttaa ttatgtttgt
    11641 aacataattt ctacctttac ccaaaatcaa acttgcttcc cttgcggtat acaagttgta
    11701 tttgtctagg ttcaatttct ttttgtttgc gatttagacc tacccctttc gcaaaattta
    11761 ttgtataata atagtaccat tgcactgtca taattgcaat atttttttgg ggtttatctt
    11821 catttaatag cctaaaaatg cttgttagat aaggaatatc taactaatca aaacaaaaaa
    11881 ggaagttaag aaaaatgaac aaaaaaagga aattatcagt attaattaca cttatttcta
    11941 tattgactat tcttagcggt atcttgtcgc tgaaaatatt caatcaaata aacacaagct
    12001 caaatgccaa agatggaata gtcgtagaaa atacctctaa ttctaaagaa actggtacta
    12061 ctacaaaaaa agacaaatct gctgagatca ctacgaaaaa ttatttaata aagactgttt
    12121 attttgatga acgaaaaaca gcaattctca aagatgagga aggatcagaa ttaaagctac
    12181 ctataacggt atccgataag ttcaatggaa cgttctcctt acaaaccgct gaaggatcac
    12241 ctgacaatct caaagttggg caaacaatca ttcttggtaa aaacgacgaa ctgtattcat
    12301 ttgtaaaata aaaaaatgga gcaaaggcta gtcaagccct tgctccattt tccattcttc
    12361 aagaggtgta gctatttcaa caccaaagtt taaatactgt ggatgctgta gatacatgat
    12421 atccattcta ttttcgataa actcttttaa cgttgagtag atgtattttt ctaatgctat
    12481 atctgcccaa ctaccataat caagtttatt cgtgatcttg acgtttattt caatttgaga
    12541 aacatcaata ttttgaacat caggaaactt attcagtctt agctcttctc taaccgcttc
    12601 tactagcggt tcatattgtc cgcctagttc gctaattgtt gaaactattt catctgaatt
    12661 ccaacgatct tccaatacct ttgctacatt ttctttaggc ggttgtcttt ctataaattc
    12721 ttcaggtgtt tcatcaaact ctactctaaa gttcaattta ttttgattgt tagcatacat
    12781 ataatcatct ctgtttgatt caaaaaagtt acgaattcct gtgtctgatt tatcacttaa
    12841 ctccttattt gctaaagtag cccaaaaatc ccaagttcct ttattttcag tatacaaagt
    12901 aaaattaatg tttatatagt ttgcagtttc aggataccct tgctctctaa gagcatcttt
    12961 aacaatcact gctaagtcag tttcaaaatt ctccataaaa aattcactaa attcttttgg
    13021 attccacgtt tcttctaata acttttctaa tttacgatct gccattatta ttctccccca
    13081 ctttactttc aatcttatat ctaaaactgc ttaaaattaa gagctatacc ttatcataga
    13141 aggtatttta tgctttattt aagtctacaa ttctatatta caattatcat ctagaccatt
    13201 ttcttatgtc aaataaacca tatatattgt ctttaatagt tttatttgac tttttaagaa
    13261 agtatttact tttgactttt tttacgtttt acaacatata cgtattgtaa tgttcagctc
    13321 aatttattat aatctaaatg aattgattaa taaaaattct gaggttcggg ttttttatca
    13381 attcttttct gtttgtgttt gtgatgaaac aatccttttt cggtaggttt tgttatcatc
    13441 gtcagcacaa agagctaacg ttggtaccgt tagctctttt tttattttac tctgctctta
    13501 catagctatc taaatcctcc aagtaaaaca attgattttc tcctatgctt gttactccac
    13561 gccaaattgt tgttgttgct gttgttggag tatttcttgt ctgtgtaatc aattcaacca
    13621 ttttaccttt ttgatttgtt gtatagtaat cagcagtata gctagatatt ggactattac
    13681 cagtgcctac catcgtgcca gaaatatcaa caggactaga ctttactatc tgaggaaatt
    13741 ctttttgtat ttccttcata ttcttcgtat atttttccca tgaattccag ttgtaaaata
    13801 aattaaagaa tttcctactt tgttccttga tttcagcatt ccccaattct ttgtattggg
    13861 cttcctgcat agctttcact ttctggttat tcttgactac ttcgatttgt ttacttttaa
    13921 ggtcttccaa ttctttgaca ttattatcat aagcattttt cttttgaaat agtaccacac
    13981 aacaaaggat cacaattatg gaaagtattc caatcgcctt tttagcagta gtgatttcat
    14041 gattttgcat aaaaagcacc cccagtcact ttcttatcat tatttagtgt gatggttaat
    14101 gtggtatcat acactttttt tccattgtat tgaacttcaa aatcggtcaa tacttcatac
    14161 aacccactac tagatgtctt aattccaaag tttgttagct taaaagtaaa aggcttatag
    14221 tactcgattt tttctttatc actactctta tcagtaattg gtggtttacc tgtgacaata
    14281 aatagttttc tcaaaaattc ttcacctgat tgatctgcaa acttattttg aactgatcca
    14341 ttcaacattt cttgaatatc attttctaaa tctttccgta atccatccac ctcatttggt
    14401 ttaacctctt taaagaatgt cagcttatct tcaatatgca cactgtgttt ttcagcttct
    14461 atgtcctgat tctgcttatt gaagaatagg attccaacaa gcagtgctaa taacatacca
    14521 gaaagcaaag aaatgataat tgtatctttc actttactat tcatctatat cgtccttcat
    14581 tccaatttat ttttcgattt caaatttagc ttttcctaca ccgctaaaga aatgcacatt
    14641 atctgatccg tccactccat caatcaaagt taccgaaact ccccttgttg gagcttgggt
    14701 tggtggaaca ttaaagttag cacatagaaa tgacccatct tcaaacaccg ctataactac
    14761 accagtatgc cctgcatcgc caacagccca taaccatcca tccgaagcac taaacccata
    14821 acctacgatt ggtttgttcg ttattttcgc tcctgccgat ttgtagcttt cccatacata
    14881 gccaccattt ccagtagtcg gttgattccc atcgtataat tggctcatat aagcccacgt
    14941 caactctgta cactgacctt gatatattcc gttaaaaggt gaagttgacc aatctggttc
    15001 tttgaatttt ggtagcgtga tcgctttccc atattttttc aaagcttctt cataagtcat
    15061 attcgctatt acttgacctt ttttaccgtt aatttttcct tctttaccca ttgatccaga
    15121 accagaatta gcatcatctg tagttgcaca ggcatcttct actgttcctg atgctgagct
    15181 tgatgaatta tttgatccac caccggtact tgttcctccg gcaattttat tgattttgct
    15241 tacatcttta gctccgtcca tgcctttgct tttcatgtat tcccaaattt cttttgcatt
    15301 tccaccacgt acagccacta cagctgattc ggaatctgct gaaatttccc aattcttatg
    15361 aaacgctacg gcatttgcca caacatcatc agaagcgttt aaagcataat cttttaatat
    15421 agcaacaaat ccaccatcgc ctttgaacat aaaatccaat tgcgcttctg tcgtccacca
    15481 agcgccacca aagtttttct tcgcccagtt tactaatgca gtatggcgac ctgctgtcca
    15541 ttgaccatag ccaataccca tcagaccatc agtgaattgt ttgataaagt caacttctct
    15601 ctgtccatca ccgtaataac cttctaccgt tcgaggatca ttacctgatt cttgtttcca
    15661 gttacctaga atacctgcga tagtagcacc tgatagccca tattccccat tcatgaattt
    15721 gtaaatatca tcaatatttt ttgctacatc acctgataca gcaccactcc ctgtttcatt
    15781 tccatcacta tcttttcctg atccactaga cccttgcgag ccttcagtag aacagttgaa
    15841 ctgtgcatca taagtagcta ttccatcagc atgtgtcact tgcggattta tcgtgattaa
    15901 actcgctgta atcccgatta tctgcatcac tgcgataagc aacgtgccga acgttttctt
    15961 cgattttttc aataactgcg cccccaaaag tgtacatttt tatgttatta tgatattata
    16021 ataacataaa taagtagaaa ggaaaagcta attaatgact gataaaacag gtaatctacc
    16081 taaactagct ccaaaaacat ctcaaacaaa gagcaaaaaa gatgaaaaaa ttaaaaatgc
    16141 tgtaaagaaa aaatcagtaa aggaatcatc ctcgttacta cttgatattc caatgagtac
    16201 ttttgatgaa atacttgatg acaatacgga tgaaacttct gttaaaattg catctcacgt
    16261 tggaatgaac agtcaaaatg attcttttta ccacttgtta aacagcctaa gagagttgga
    16321 agataaaaaa aatcaggtaa tcgcatcaaa agctcctgac ctcacaaccg atttggatgc
    16381 tgaaattatc caagtcaaac aagatgagcg atcagttaaa tcgctactga aaacctattt
    16441 tgatggtttt gataaagaac tagctccata tagccaagca atcctaacta aaccaataca
    16501 agctccacat atttcaattt ccgaacaaaa aaagagtgat gactaaatag tcattactct
    16561 tttttcaaat tttgtttatt aagga
//
LOCUS       DAZUMO010000058        16526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000058 DAZUMO010000000
VERSION     DAZUMO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.058"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..726)
                     /locus_tag="ACG8ER_001999"
     CDS             complement(28..726)
                     /locus_tag="ACG8ER_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS5050573.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="ACG8ER_002000"
     CDS             complement(719..2071)
                     /locus_tag="ACG8ER_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS5050574.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="ACG8ER_002001"
     CDS             complement(2294..3694)
                     /locus_tag="ACG8ER_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5050575.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="ACG8ER_002002"
     CDS             3855..4592
                     /locus_tag="ACG8ER_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS5050576.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="ACG8ER_002003"
     CDS             4717..5196
                     /locus_tag="ACG8ER_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS5050577.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="ACG8ER_002004"
     CDS             5193..5960
                     /locus_tag="ACG8ER_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS5050578.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="ACG8ER_002005"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="ACG8ER_002005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5050579.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="ACG8ER_002006"
     CDS             complement(6863..7465)
                     /locus_tag="ACG8ER_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5050580.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..7801
                     /locus_tag="ACG8ER_002007"
     CDS             7571..7801
                     /locus_tag="ACG8ER_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050581.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7792..8040
                     /locus_tag="ACG8ER_002008"
     CDS             7792..8040
                     /locus_tag="ACG8ER_002008"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5050582.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8146..8730
                     /locus_tag="ACG8ER_002009"
     CDS             8146..8730
                     /locus_tag="ACG8ER_002009"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS5050583.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="ACG8ER_002010"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="ACG8ER_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS5050584.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="ACG8ER_002011"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="ACG8ER_002011"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS5050585.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="ACG8ER_002012"
     CDS             10631..10810
                     /locus_tag="ACG8ER_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050586.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="ACG8ER_002013"
     CDS             10832..11857
                     /locus_tag="ACG8ER_002013"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS5050587.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /gene="glyA"
                     /locus_tag="ACG8ER_002014"
     CDS             12032..13276
                     /gene="glyA"
                     /locus_tag="ACG8ER_002014"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS5050588.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="ACG8ER_002015"
     CDS             13429..14376
                     /locus_tag="ACG8ER_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS5050589.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="ACG8ER_002016"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="ACG8ER_002016"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS5050590.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="ACG8ER_002017"
     CDS             complement(15278..15520)
                     /locus_tag="ACG8ER_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050591.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="ACG8ER_002018"
     tRNA            15641..15713
                     /locus_tag="ACG8ER_002018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="ACG8ER_002019"
     CDS             complement(15832..16470)
                     /locus_tag="ACG8ER_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS5050592.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5634 a         2949 c         3324 g         4619 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagtt
//
LOCUS       DAZUMO010000059        16501 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000059 DAZUMO010000000
VERSION     DAZUMO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.059"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..673)
                     /locus_tag="ACG8ER_002020"
     CDS             complement(<1..673)
                     /locus_tag="ACG8ER_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5050593.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYK"
     gene            complement(793..1440)
                     /locus_tag="ACG8ER_002021"
     CDS             complement(793..1440)
                     /locus_tag="ACG8ER_002021"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFS5050594.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1524..2951)
                     /gene="uxaC"
                     /locus_tag="ACG8ER_002022"
     CDS             complement(1524..2951)
                     /gene="uxaC"
                     /locus_tag="ACG8ER_002022"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5050595.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3025..4104)
                     /gene="uxuA"
                     /locus_tag="ACG8ER_002023"
     CDS             complement(3025..4104)
                     /gene="uxuA"
                     /locus_tag="ACG8ER_002023"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5050596.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4107..5738)
                     /locus_tag="ACG8ER_002024"
     CDS             complement(4107..5738)
                     /locus_tag="ACG8ER_002024"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5050597.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6042..6713
                     /locus_tag="ACG8ER_002025"
     CDS             6042..6713
                     /locus_tag="ACG8ER_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5050598.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARRFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(6873..7064)
                     /locus_tag="ACG8ER_002026"
                     /pseudo
     CDS             complement(6873..7064)
                     /locus_tag="ACG8ER_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(7432..7773)
                     /locus_tag="ACG8ER_002027"
     CDS             complement(7432..7773)
                     /locus_tag="ACG8ER_002027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050599.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            complement(7870..8322)
                     /locus_tag="ACG8ER_002028"
     CDS             complement(7870..8322)
                     /locus_tag="ACG8ER_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050600.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(8329..8877)
                     /locus_tag="ACG8ER_002029"
     CDS             complement(8329..8877)
                     /locus_tag="ACG8ER_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS5050601.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(8922..9254)
                     /locus_tag="ACG8ER_002030"
     CDS             complement(8922..9254)
                     /locus_tag="ACG8ER_002030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050602.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(9268..9525)
                     /locus_tag="ACG8ER_002031"
     CDS             complement(9268..9525)
                     /locus_tag="ACG8ER_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050603.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(9720..10262)
                     /locus_tag="ACG8ER_002032"
     CDS             complement(9720..10262)
                     /locus_tag="ACG8ER_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFS5050604.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(10283..11374)
                     /locus_tag="ACG8ER_002033"
     CDS             complement(10283..11374)
                     /locus_tag="ACG8ER_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5050605.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(11382..13592)
                     /locus_tag="ACG8ER_002034"
     CDS             complement(11382..13592)
                     /locus_tag="ACG8ER_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5050606.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(13608..16136)
                     /locus_tag="ACG8ER_002035"
     CDS             complement(13608..16136)
                     /locus_tag="ACG8ER_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5050607.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(16188..>16501)
                     /locus_tag="ACG8ER_002036"
     CDS             complement(16188..>16501)
                     /locus_tag="ACG8ER_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS5050608.1"
                     /translation="VNAATFLLVVIFEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCV
                     LALAKLKPDGKKVHFYLWDWFCYMIRMMNGDCVYYNWDKVSNNALEELLFVNKYVYLE
                     D"
BASE COUNT         5227 a         3408 c         2279 g         5587 t
ORIGIN      
        1 ctttgtattg agattggtca attcttactc gttcagtagt tagtttataa catattaaat
       61 aacaaataag tgcacatatc gaaaatattc ccgcaacaat tgtaaaagtt gatccaccac
      121 gaactatttg agcgcctgaa tcatctcttg tataaataaa taatggtgtt aatacaccaa
      181 tgaccaaact tgctaaagta gctccaacat ttcggaatgt cgatagcatt gttctttctt
      241 ctgtaataga agtaatagct gatgccattg atccataagg aatatttata gatgtgtaaa
      301 aaatactccc ccataatata tacgtaacat acatgtatac taccttaacg ctcatagaag
      361 ctgaaatcat agaagtttga tacattaaaa aacttgctaa agcgactgga ccactcattc
      421 ttaaaatcca cggtttaaac ttaccctgct tagttgcttt cgatctatca acaatacgcc
      481 ccatagttac atcagtaact gcatcgataa atcttgctac taaaaataat gttccaacgg
      541 cagctccgct tatccccaat acttttgtat aaaagatcat taaaaaagaa ctagcaaaaa
      601 tgaaagtaaa atcgtttcca aaatctccaa acatatatcc tattttgtct cttataccaa
      661 attttctttc catacaattc tctcccttct gttgttaagc gttttcattc tgatgaaagt
      721 acgtactata ctctgaagcg taaagagtca gtatttattt tttacatttc agagtattat
      781 ttgttttttc tcctaataat tctttatctc ataaaactta tctatatatt tactagctac
      841 tcgacttact tcttcaaaat cacctttttc aacagaagcc aataaattcc cacctacacc
      901 aactgcaaca actcctactt caaaccaatc ttctaagttc tctatactta ctccgccagt
      961 aggcattata tttacgtgtg gtaatggagc ttgataggct tttacaatgg atggaccaaa
     1021 agcagaacct gggaatagtt taatgatatc tacgccagca gtaagtgcct cctttatctc
     1081 agtaattgtc atacaacctg gtaaataagg aatttgatat aaattacaaa tctcagctgt
     1141 ttgtcgatca aaagatggac taacgatata ttctgcccca gccataattg cgatacgagc
     1201 agtaaacgca tctaaaaccg taccggctcc gattattaca tctttttgat cagcatattt
     1261 tttagcaatt tctttgatta tttcttcagc attaggaact gtaaaagtta gttcaatccc
     1321 tttaattcca cccgcaataa ttgaatttat tgctttaatg gcctcatcct tatttttccc
     1381 acgtacaacc gcaatgactc cagccgcttc taacttactt aaaatttcta cttttttcat
     1441 atctatctcc ttcctttatg gatacatact ttgtctagct gtactaactt acaaagaata
     1501 aatttaagct tttatttcaa gtattatttt ctacttccga tttcaaaaaa attaaaataa
     1561 ttatacgcat tacgataaca aatattctcc actatctgac taagatacat ttcatcatct
     1621 ggaattcggc ctgctttaac ccactcgctt agtaattgac ataatactcg acgaaaatac
     1681 tcatggcgag gatagcttag aaaacttcga gaatcagtca acatacctgt aaaattcgac
     1741 aacaaacttt gttgtgccat cgtagtcaac tgttgacgca ttccttcagc tgtatcattg
     1801 aaccaccaag cacatcccaa ttggattttt tgaatcatac ccccttggaa gctttgcatt
     1861 gctgttgcta gtggaagcca atcagttgga tttaatgaat ataaaattgt ttttggtaag
     1921 cacccactct tttcagcggt atcgaacaat gctacaacat tttgtgtcaa actagcttga
     1981 tcccccatcg aatcaccacc gacatctact ccaatttgtt cctttaatat acttgaatta
     2041 tttcttaaag catttaaatg catttgcatc gtccaaccat atcttgtata ctcagacatc
     2101 aaatgtaatt gaatacctgt aacaaattgt gcaatttctg ataaatggaa atccgttttt
     2161 cctgctaaag cttttattaa cagattgtcg agtatttttt catctgctat ttcataaaaa
     2221 taagtattca aaccgtgatc tgctaatcga cagccaattt gatgaaaatg atccactcgt
     2281 tggctcaatg cttgcttcaa cccattaaag tctttgattg gtatagccgc tgtttgacta
     2341 agttttaaaa tatagttcga aaagcctgat gatttgatac tccagacctt atctggacgg
     2401 aaagtaggta ataccttgaa agaattatcc tgtttagaaa gctcagtatg ataccacaag
     2461 tcatctgtag gatcatccgt tgtacagatg gctttcacat ccatcatctc caacaattct
     2521 ttcgggcgat aggaatccgt ttgaattttt tcgttagcac gtcgccaaat ttctggtgca
     2581 ttcttctcag aaattgtcaa atcaatatca aagtaacgtt gtaattccaa atgagaccat
     2641 tcaaaaatag gattacctgg cgccttctcc actgttttta caaaagctaa aaatttttca
     2701 taatcatcac ctggaccact gatcaactct tcttctatac cattagctcg catcaatcgc
     2761 catttataat gatctccagc tcctttatca tttaaccata cctgagtcaa attcttgtat
     2821 tgcttatttt ctagcaaata tgctgggtct aaatgacaat gatagtcgat aattggttgg
     2881 tgttttgcaa atttatgaaa aagtctttta gctatttcat tctctaataa aaagttctta
     2941 tctaaaaaca tatttaaaca ctcctttcct tccgttagtt tttagaagag aaaatttttc
     3001 ctctaaataa actcccttat ataattattt actcgccccc ttttcaagcg cttcccaaat
     3061 accatttaaa taaactgcac ctagagctcg gtcatataat ccataaccag gtctaccggt
     3121 ttctccccaa atcatccgac catgatctgg acgaatatag ccattaaatc cattatcgta
     3181 cattgttttt aatatcttat acatatctag agaaccatat tctgatttat gcggcgcttc
     3241 gtaaaaatcg agtccattgt cacttaaata tttgatatta cgaacatgtg caaaaggaat
     3301 ccgatcccgt tgacaaaatt cagatagaat ttcatagaca tcattatctg gtcgttctgc
     3361 aatacttcca gtacataatg taataccatt agaaggagaa tccactacat tacacaacca
     3421 atctaaatcg tcacgatttt taacaatacg aggtaatccg aaaatggaat atggtggatc
     3481 atctgggtga actgccattt taatcccaac ttcttcacat gtcgggataa ttgctttgag
     3541 aaaatatgca aagttctctc gtaatttctc ttcatcaact tctttatagg cttctaataa
     3601 ttgcttaatt tgtgataacc gatgaggttc ccaacccggc aattcaaaat tattagtaga
     3661 attttctact tctctaataa tttcctccgg atcagtaggg atattttttg aaatataggc
     3721 taacgtagaa gacccatcat ctaaacgata atccaaatcc gattttaccc aatcaaatac
     3781 tggcataaaa ttataacaga taacctcaat cccaaattct gccaaattgc gaatcgtctg
     3841 tttataattc tcaatgtatt gatctcgtgt tggtaaacca attttgatat catcatgaac
     3901 attaacactc tcaataactt tcaaggtaag tccattggct tcgacttcat cttttaaaga
     3961 tttaattcgt tctctcggcc aaacctctcc ggctggtata tccattaatg ttccaacaat
     4021 tcctttcatt gctgggatct gacgaatttc ttgtagtgag attttttctt catttgcccc
     4081 ataccatcta aaagtcattt ccatagttac tttcctccaa cttctgttat atatttattg
     4141 attgttttac gaactgcttg aggacctact agcatttcgc aaaagatttt ttcagttttc
     4201 tttcctaccc caacttgata taaatctaaa ccaaagattt ctttattttc taaaatttcc
     4261 tgtaccgcac aatgaatctt tgaaggatct tgttcaccta atttcaatac tgataattta
     4321 tcctgcaact ctaccaataa cggatcagga cttggcacaa aattttcccc ttgatcatcg
     4381 actgccagta aataacgtaa ccaagcagca atagcgatag gaataaatgt tagattttct
     4441 gcatttccgt ctttttcgac atacttttta attgtttcac catagcgaat cgctactttt
     4501 tgagaagtat ctgttgcaat tcgttgtggt gtatctggaa tatagggatt aggcaaacgt
     4561 ttattaatta cttcatcaat aaattgtttt ggatcaataa tgcctggatt ttcaactact
     4621 ggtaaacctt ctaaataacc tattcctttg attaaaccaa ccaaatcatc atcctgcatc
     4681 tctgctgaaa ttgattgata tcctagcaga caacctgtta ctgccaaagc agtatgaagt
     4741 ggattcaagc ttgtcgtaac cttcatgatg tctgctttat caaccgtctc tctatctgtc
     4801 ataatcacac cagccttttc caatttaggc cggccatttg gaaaactatc ttcaataact
     4861 aaataatgaa ctacctcagt attagtgaaa gcagcaatat tggtatgttt ttttgtgtga
     4921 atgatatcta aatcctcgag ccccatcatc aatagatctt cctttacatt ctctgaagga
     4981 tttggcgtaa ttcgatcaat catactccaa ggaaaagtca ctttttcttt atcctttaaa
     5041 taagcaataa atttttcaga aacatatcct tctttttgcc aagcttctgc aatcgtaaaa
     5101 atactatctt gaaaacgttg accattctgt gagaaattat cagtagaaac catcgcaatc
     5161 ggcaactctc cagcattaaa tctgctatat aacaaggatg ttacaatact catcgtatgt
     5221 tgaggattgc taggtccaga tttgatatca gctttgacta ccgaaaagaa ttgcccttta
     5281 gaatctttta aactgtaacc tttttcagta atagtaaacg ttgtaaactg taatttagga
     5341 tcttcaaaat atttaaccac tctttgatag tccgctatat tcttcggatt gcaatataaa
     5401 ctatcagccg ttactgccaa aagtcttttt ttgagttcac catctccact cataacaatt
     5461 tccaaaatgt cattgttata gtcatgataa gctttttcca ctacttcttc atcaaatgtt
     5521 tcacacacaa ctactcctgt ttctaattca ttttgatctg ctaagcattg agcaatttct
     5581 gcatgaaatc ctcgataaag atttcctcca ccaaaatgaa gccatactgg atgttgctgg
     5641 ccttcttgct taacttcttt gacatcaaaa cacggcagta gaatctgcat ttcattgaat
     5701 tcttcgatag aattttgcca atttttaatc gaaaccataa ttaacctcct caaactgata
     5761 tgttagtttt gatacatttt atattttaaa acccattgta accgttgtca atataaaaag
     5821 ggaataaact tgcttttttc taccatatca gaatgattgt attttttatt cagaaaaata
     5881 aaatactcct tctttttgga ctgattttat cgaaacttat tttttctttt tttcatgtat
     5941 tcttttattt ttataactag tgtattactt caaaatttga tattatatgg catgaacaga
     6001 attaaaatat cagttaaaat aaaagttaag gagcattgtg tatgacaatt aaaactagtc
     6061 tccaaaatca agcttaccaa tctattcgcc gacaaattat ctatgctgac ttagaacctg
     6121 gaaaaaaagt atcagaaaaa aaattagaag aaacactttc tattggtcgt acaccaatac
     6181 gtgaagcact gattcaactc cgacaacaag gtcttgttga tacaattcct caaagcggaa
     6241 attatatctc attaattgat ttagattcgg ccaaaaatgc tcgatttgtc agagaacatt
     6301 tggaacgaca aattatgtta gagtgttgtg ccaaaatgaa taaccaaaaa aaacggattt
     6361 tagaaactat aatttcagaa caagaaaaag ctgttagtca aaaaaatgct cgtaggtttt
     6421 ttcataatga taatttattc cataaagctt gttttgaaat tgcagagcga gaagaagtat
     6481 ggcaatggtt agaaaataac aatacacact tagaacgatt tcgatggcta cgagtaaaaa
     6541 cggaaggatt aaagtgggaa aaaattatgg aacaacatta tcaactattt gaggcattaa
     6601 caaaacaaga tccagaagaa gctaactttc taacatcgtt gcatcttcat ttaatgctta
     6661 atgagcaaaa tattgtttta gctactcatc caaaatattt caaaaatata taacttttaa
     6721 ctctttttaa attaaatggt tgttctgatc agaaaaatat cgcttgtttt cttattatgt
     6781 aatatatttc ataaactatt ctgcataaca actattgttt gtacttttta cattggataa
     6841 ctaattatga ctgctgataa cttttgatat taaactagta tctccaatac aaataaaaaa
     6901 tagatatctt gttcatcaaa attagttcgt ggatacttac cttcatttac atcattcagc
     6961 ggtttcccat tttcttttat tctattattt cattttctaa tagacttggc ttcatttcgc
     7021 aatacttttt ttcaataggc atcaaattgg tgttgtttag atatttagtt gtagttttgt
     7081 gacatgataa gtatgctcct ttttctcttt tccactaata caacgatcaa agggtgcttt
     7141 tttcaaaaaa ctagaaaaat caaaataaga ttcgtatttt gattctatat atcctgtttc
     7201 aactacatct tatatttcac aatagaataa atagcctttt gcatagctgc ttaacttata
     7261 agaaattgca atttacttct tcaaagtgaa aacgaatatg agtaaaataa aattattctt
     7321 cttacagata attaaaaata gcgcgtaaca aaaatcggca ttcaatcaca taattaggat
     7381 tgatgccgat tacttatatt ctacgtatag ttttagtatt cttatttttt atcaccagta
     7441 accaattgag aacagcccta aagcagaata gtcttttatc tcaaaacgct taatttcagg
     7501 cttaggaata tgaataatct tttgagtata ttctttctct tttaatctta ctgaagaaat
     7561 accactgcct atctttttta caaatatttc atttggtgta cagactaata aaacaatatt
     7621 tgtaatcaat ccttgcacta aagaattaaa aggaagacta tttaaaacag ggtgtgcata
     7681 actataacat gtgaagtagt tattttgaac tttaactttt aaaaacttta aaaataatct
     7741 tatttgttct tctaaacaat catctatact catataatca ctccactaat taatctcccc
     7801 aaaaagacac agcattaaga gatataccaa cacaacagtg caaataactc tacatcttcc
     7861 ttcgcaacct tatttcaata aaccataaaa attatttttt aatagaaact gaaaattaaa
     7921 actagaaaaa gtagttttac cgaatgtaag agcatcatca ccatcaatat agaaatacat
     7981 tcttttccag ttacatttaa tttttaaaca aaattctccc caaaaaggaa ctttttctag
     8041 tgaaaacttg gtaatctctt tgtaattaat tcttgttaaa gaataattaa acacttcatt
     8101 tttacttgtt attttttcat tgactataac tagttcatct ggtaaaaaaa ttaaccaaca
     8161 aacatcgaca aacagtaaat gatataatcc atttccacct ccaagcatat tcaatactgg
     8221 tgaacatgcc ttagaaacac gaatatagtt atggtttaaa tagatatttt ctttaaacat
     8281 tgcttgttta aaagccatat gctcttgttc taaacttttc ataaactttt actctaatgc
     8341 acagctagca gcagctgctg caattcctgc tacaatattt accttagcta actctttagc
     8401 tgcagctttt cctaattgtt tttctgcaat ttttaataat aaatccgcag ctttttcaaa
     8461 attaaaagct tttaatgctt caataaaatc tccctgcgta cctatagccc aagccactcc
     8521 tcctccagga agagaattaa tggctacaca tccagcatat cgtttccaat caactttctc
     8581 cattctagga acaataatat gttcataaaa gactcttgct gctcttcctt gttcgttatc
     8641 ttgactgatt cgttcacgaa tagcaccaaa atcctttata tggtatacac catgttcgtc
     8701 taaaaaacca actgtatcaa aataaaaagt taattcatct actaatttat tctttatttc
     8761 taaatcaact gtattcactt tttctgtggc atacacactt gttccagtac taacaaaagt
     8821 acttaaacct aataagaaaa cacctaaaac accaaatatt ttaattattc ttttcataat
     8881 ttctcctcca taaaaaataa gttacttttt aaatatcttg tttatgaatt cattccccaa
     8941 aggcctttta agattgatac agcagtccgt tcacttcttt tagacttgtc atacaagtct
     9001 tttaaatgat cccatatcag actagtttgt ctatctagct tcaatccatt aaacgctaat
     9061 gaacggttca ttttttctat tactttagat aaagctacag aaatttttat accttcacaa
     9121 cgcgcatctt ggatttcaat aatagtatca tcaattagtt tttgaatttc ttgcctatca
     9181 acatattcaa tcttagaaag agaatctttt aaagctacta atgattcaac gatttctttt
     9241 ttctcttcag acattttctt cctccttcta gtgacccgta aagccatgta gagcagagtc
     9301 cattgaacca gtaattacac tatctacaac gccattccaa tttactctac attttccatt
     9361 tttcagattt gtcggtccat acggatagcc tttttgacat tcaatgttcg gattatacca
     9421 gataggatta taccattcag ccgcagaagc agattcagtc gatacaaaca atcccacacc
     9481 aacaaatcca atacacatca cactatagcc aattagtttt ttcatctcgt atccatctcc
     9541 taaaaaatag aatagttctt atagttattc ataagacata ttttttccgt ttttgtttta
     9601 tcctttctgt cctataacaa cttttttcaa gtaaagaatg acatagtttt tcttaaaaaa
     9661 acagtctgtt tttttctatt tcttcggtgt ataataaata tgagtgttca ttttttattt
     9721 taatacgcac tccgtacata ataaatcttg atcgcatcaa tcttttgagc ttcttgatct
     9781 actttcaatt gaagtaatac aaattgagta accttattca ttgtttcttc tccaaataac
     9841 aaatattgat ccggattctc tatatcttgt gtcacctggt aaagtttccc agttgttttc
     9901 atttgtacat gcgggtctac atcaatacta ttgtctttaa tacaacgatc tgtcatatag
     9961 tccctcacag cttgattgcg tttataaaca tctgtgaaat tactccattt tctagcaaaa
    10021 tcttttaaga aattttttgt catttcatct atttgcattt gttgcgctgt ctcgtcttct
    10081 aatgaaccaa tttgactgtt actttcagta ctttgaagag ttccacttgt ttcttttact
    10141 gcttcgattg tttcttctgt cgttctcaat tgtgtttttt gtttttcaga agaaaaccaa
    10201 tgacaaccag taagacctat caaacataac caactaaata ctaaaattat cccttttttc
    10261 acgtttctag tcactgcctt tcttatttca ccttacgaac cagactacga atttttgttt
    10321 gattgtacgt gcgttgatac cgagccataa tccgtccttg ctcggcattc tgttccacac
    10381 tagagaaatg ttgtccgcca tatgaaacat ctgttataat tccggtgtga ccatagattc
    10441 caggagacaa tgcaccacct gcttgccaat tcaccacatc tcctgcttgt aaatcacttg
    10501 gttttgggtt catgattact tgccaaccat aggttgtcca gttataatct tcccctatta
    10561 acatcgcaaa catgtgtcca gaacccatta actgtgggcc acctaattga gatacatacc
    10621 atgccgtacc accataacat tccccattat ttacggatcg tccaatgatc cgatccaaaa
    10681 ccttcgcatt gcctttactt tttcctgcaa taagcgaagt tttttttgtt ggtaggctat
    10741 tccaatgctt gatcgcaaaa gtatcatatt ccgttaagtt ataggacgtt atgatactca
    10801 ttgctcctcg ataatacgga ctcccttcgg taatacctgc tgccgcatag cctccacgac
    10861 ctagaatcaa taaacaactt ccaggatctt tattgcccac acattgatta aagttgcttt
    10921 ggctcgccac cataaagcca taatagtcat aacaagcttt taagttcgga aacttcatat
    10981 agaaaccacc ttcagaacca ccaattccac gtgcagaacc ttgtgggtag ggacaaccag
    11041 gaaaccaggt aatcccaaaa aagttcgtat cgtttcttcc agattgtgac tgtccataag
    11101 ctgattcata gcctaattga gaaagaatca cactcggtaa taactgatac tttacagctg
    11161 caccaactgc tgcttgcgca tgttcatctg tgattggacc acgtccttta atctctgaaa
    11221 aaatcggtaa tccttcggta tattccccat taaaagcgac agctagacct gtagcttctc
    11281 ctccatgctc agaaagttct cctgaaaaaa agaatggcaa tagaaaaaag ggaagaataa
    11341 agaaaagaag acaacctaga caccctttct tcatttcttc ctcatccttt cttcgataat
    11401 ggtttttgga ctgttcgtcc ttttggtaaa gtaaaatcag ttcctggttt tgcgtccaaa
    11461 atcggcagtg ttttaggatc cttttctatt ttttccgatt gctctgttgg cttatctaaa
    11521 gaagaaaatg caggttgtaa cgattttggt gcggaaatcg acggtttact ttcagacgaa
    11581 cgttgccccg gttgttgcac agaacgattt cctgatagag tggctaccga cgaattagga
    11641 acagagtatt ggttcaataa ctgctgcatt gctggataac tcccattttt cgcctgattt
    11701 ttcacttggc gaatctgttg tgaaagagaa ccagcagctg ttaattgtgg cacgggtact
    11761 tttccagaag gcaacggaat cgattgacca acaaaagcga tacctttatt taacaatggt
    11821 tttctttgat tattcccttg ttgcttactc aattgtgcca atgattgtac ctgttgtttt
    11881 ggtcttactg aattcatgga cgaatttgga atcaaaccat ttgaaagacc agaattccac
    11941 tctgttttcg atttggtaga agacatttca ggctctaccc catgataacg aacatgacga
    12001 ccactataag gcaaggcttg atttgaacgc ccacctagtt caaagggatt aaccacttgc
    12061 ctccctttta ccgattcttg tggtgtacgt ttttgttgaa acttttgaaa tccatgtatc
    12121 gctgcagaag cgagtggatt cgttgctcga tacacattcc ctgctaatcc tgcacctttg
    12181 attgccaaat tttgtccagc agttgatagt tgaccaggca gttgtttcat ttgagacaaa
    12241 gaatgacttg ctatgttttt cgtgttgttt atagtttgat ctgcagctcc taaccctagt
    12301 ttctttaata aatctttccg ttttacaatg agtaaaattg ataggagtgc tttgacaaac
    12361 atatttccta aaaaaccaaa aacagtattc attggaatta aatcgtctac tatgttatag
    12421 atcaaaaatg ccaccaaaat aatcacactg taaaaagatt tttgaaacat caagcccaaa
    12481 aaggacttta aggtattcat gagcatataa tcaaatactg gaacaaaaga agcgagtaat
    12541 gaaaaaggaa tcacaagtaa taaacacaac accaacattt gaaagataaa acgaatcatg
    12601 ccaatcacta caagtggtaa tccaatactt agattaagca atggcaccat agaaccaacg
    12661 actgctttat aaacagcttt agttggttga atatagacac gataagcctt ctttttccct
    12721 ctatcttctc ctgcagcagt ggtcactttt gtctcaatat cagataattc ttcacttgac
    12781 gcttccttgg ctagaaattc ttgtggttca atctctgctt gatacaattc ttctaaattt
    12841 gttgtcccat aatttaacaa tatatacgga tcaacaacag ccgctttata ataaagttgg
    12901 cgcacttttt gaatggcttc cttttgtgtc gttggcacta aaacaccatt gacatctttt
    12961 gtcgccaatt catctagact atttgtagca gaaaaaataa agccttctac ctccacggac
    13021 aaatcattta accctttggt aaaagcttct cctttactag gtacgttact accatagcca
    13081 aaccaaacca ttgaaaaaat gacaacgaag agaaatcgaa taaacaccat ttttgcgtaa
    13141 tgtttccctc taaatatacg aagagtaaat agatataaca catacaagac gacaatcccc
    13201 gcaccaatag gatcgtatat ttttgaataa atagtatccg aaaaagtgaa aaactggtga
    13261 attaactcgt tcattgcagc tccttcatag agcttctcaa tgacaaaatc aaggccttgc
    13321 caaatcaatt tacttaaact aaagaacata ttcgtcatca tttttagaaa atctgtgcca
    13381 tttgccccaa ataacatttt gcctaatgaa aattcactat cagataaata agaagaaaag
    13441 gtatcaatat gaaatttccc aatctcatag atcgattgat ccacctgctt ttctgccgca
    13501 tacaccaatc caccaacaaa aagcagtccg aatccaatcg ttaaaaaaaa caaaacgcac
    13561 catttctttt ccttatgctt tatataagcc attgtttcac ctcatttcta ttgcatgatt
    13621 aacgtatcct gttcttctac ttgagagtga cttgtttttt ccgttgtttg taatgcagcg
    13681 gtcatttctt caaacaaact gtgtacattg agttttccta cacgtccata gggatctaac
    13741 atccaacact gtcctttaat catgtggctc atttcagcta agttcgcttc atttacttcc
    13801 aaacgtaaat gattcaaaat cttttctcgc tcactgtctt catcaaaagc aaagacgaca
    13861 ccaaaattcc ctttttcttt atctgaacct aagtcaccaa taaattgtgt atctaacact
    13921 aattgattca tctgggaacg ccccacacgt gccattgctg caataatact acgtcctgcg
    13981 ttcgaattat taaataccca tgcttctgta aaataaatcg ctgttttctt actactatct
    14041 cgttttccaa acatttcaca aaaacgacct aaactaatca tcaaacacaa agactttcga
    14101 tccgcatctg tatatagttc tggtcggaca ttgtcatttg gtaatttcaa atcttggatc
    14161 tctaaaatcg ttgttttggc attaaagtct aatcccgggt tttctccata agaaaatcct
    14221 aaacgtaaaa tcgaatcttc taccgtcaag cgtaaaaact caccatattc tcgaacctct
    14281 ttttgttcca tttgttctaa atagtcgacc acatgcatca agccaacttg ttgtccggct
    14341 tctttttgaa ctaaaacttc ttggattccc tttaataccg ccccttttaa aaggtgattg
    14401 gtacctaacg gacttaactc atcaatcata tcctgggcta cttgtttcgc atctactcgg
    14461 gataaacaaa caattggatc taatacacca taattacggg gatttgtagc atctaacgtg
    14521 acataactca ttttttgcaa atgttctaca aacaaagggt aatgtttctg ataatacggg
    14581 tcttgaatga gtttcgtaaa ccatttgcgc ttttctcctt tgggatccac aaataaactt
    14641 tgtacattca taaacgaact atacaaaaag agcaagccaa ctaaaaaggt cttcccttta
    14701 ccggtttttc cagttactgc tacatgaggc gcatcaaaca acgcaccttt gattccttcc
    14761 gcaatggcta gaagattcaa aaagacaaaa cgatttgacg cataaattaa ttcttctaat
    14821 ggtgtttttt ctcctcgaga accaccatcc cctaatgtat cttgccgacc aatataccag
    14881 ccggattcca tacctaattc tgatactgct gaaaacaacc attcgccaac tgcttctaca
    14941 gttgttcggt gcttccaatg taactgccgt cccaatggtt gccctaataa acatttatag
    15001 aacaattctc gttgatcatt ttttgcacgg tggatatgga taatcgtatg aagttgttga
    15061 ataaccgttg cactattttg acgaacttct tcaaccgttt gaccgtatac aataaaacat
    15121 gcgatccaat cgacgacagg ttgcccatta tctactcgtt gctttaaatg ttttactaat
    15181 cctcgaacag ttaataagcg ttcactaggt tcttcatcat tttgaaccaa ttccttatgc
    15241 tctgttttga accgttccga tagccaattc agttttaaac caatacctcc ctgtcctttc
    15301 aagcgtggat agtgaatttt cattcgcaat tctgctggga aagaaagatc ctgagcaaat
    15361 tgaaatagat cacaatttct catattttca ggtgtttccc caataggcaa caatgctaaa
    15421 taactactcc ctaactcatc ttctaattga agaaggcctt tatgttcctt cggtatacgt
    15481 acatacgttc cttgattgtc tttcagtact tcccaattat tttgttcttc tactttgtga
    15541 gccattcctc gaatatattg cagacgattg agatacaaca gctctttttt tactaaggga
    15601 cgaatagaaa agctataaag taattgtgtc acttctttta cataaggatc actttcagaa
    15661 aacaaagttt ccaaatcatc tacttctaat ccaaataagc gtgctaaact tcctgctaaa
    15721 tctaaccaga ttagatttat tacatcatga aaactctcca ccgttgcttt ttctttgact
    15781 ttgattccta aaataaatga aggacgatag acttgacctt ctcgttccaa caatgtcact
    15841 gtttcttttg cataatacgc accaattgct tgactatcgg gagaaaatcc ctttcctaat
    15901 gtgtcaaact tcgtccgtaa attcattgtt cgtggataca tcaccaactc cacatcttca
    15961 taatctgcta aaagcaacag taaacgagcc atcatttctt ttatttcctg tgtctttttt
    16021 ggtgtgtttt ttgaaattgt tcgtggaaaa acatgatagt aggcccagat acaatcctct
    16081 ccatttaaca aacaattttt cttgaatgct tttactggtt cttttacaag caccaatgaa
    16141 tttcacctcc caataaaaaa ggaacaacca gttgattgtc cctttttcta atcctctaaa
    16201 taaacatact tattcacaaa aagtaattct tccaatgcat tgtttgatac cttgtcccag
    16261 ttataataca cacaatcacc attcatcata cgaatcatat aacaaaacca atcccataaa
    16321 taaaaatgca ctttttttcc atcaggtttt agttttgcca gtgctaacac acataaatag
    16381 gggagtccta tataaaaaac aactcctgct ggtggaaaaa aagaaatgac ccaaccaata
    16441 aatgaacgta aaaataaata ttcaaagatt acgaccaaca aaaaagttgc tgcattgact
    16501 a
//
LOCUS       DAZUMO010000060        16486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000060 DAZUMO010000000
VERSION     DAZUMO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.060"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1238)
                     /locus_tag="ACG8ER_002037"
     CDS             complement(<1..1238)
                     /locus_tag="ACG8ER_002037"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5050609.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKM"
     gene            complement(1231..2823)
                     /locus_tag="ACG8ER_002038"
     CDS             complement(1231..2823)
                     /locus_tag="ACG8ER_002038"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS5050610.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(3333..4058)
                     /locus_tag="ACG8ER_002039"
     CDS             complement(3333..4058)
                     /locus_tag="ACG8ER_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050611.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(4212..4538)
                     /locus_tag="ACG8ER_002040"
     CDS             complement(4212..4538)
                     /locus_tag="ACG8ER_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050612.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(4575..4940)
                     /locus_tag="ACG8ER_002041"
     CDS             complement(4575..4940)
                     /locus_tag="ACG8ER_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS5050613.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(4977..5192)
                     /locus_tag="ACG8ER_002042"
     CDS             complement(4977..5192)
                     /locus_tag="ACG8ER_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5050614.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5321..5656
                     /locus_tag="ACG8ER_002043"
     CDS             5321..5656
                     /locus_tag="ACG8ER_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5050615.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(5732..7729)
                     /locus_tag="ACG8ER_002044"
     CDS             complement(5732..7729)
                     /locus_tag="ACG8ER_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050616.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(7827..8102)
                     /locus_tag="ACG8ER_002045"
     CDS             complement(7827..8102)
                     /locus_tag="ACG8ER_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050617.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            8334..9068
                     /locus_tag="ACG8ER_002046"
     CDS             8334..9068
                     /locus_tag="ACG8ER_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5050618.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            9107..10249
                     /locus_tag="ACG8ER_002047"
     CDS             9107..10249
                     /locus_tag="ACG8ER_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5050619.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(10877..11362)
                     /locus_tag="ACG8ER_002048"
     CDS             complement(10877..11362)
                     /locus_tag="ACG8ER_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFS5050620.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(11364..12800)
                     /locus_tag="ACG8ER_002049"
     CDS             complement(11364..12800)
                     /locus_tag="ACG8ER_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFS5050621.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(12833..13684)
                     /locus_tag="ACG8ER_002050"
     CDS             complement(12833..13684)
                     /locus_tag="ACG8ER_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFS5050622.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(13840..14085)
                     /locus_tag="ACG8ER_002051"
     CDS             complement(13840..14085)
                     /locus_tag="ACG8ER_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5050623.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            14190..14963
                     /locus_tag="ACG8ER_002052"
     CDS             14190..14963
                     /locus_tag="ACG8ER_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050624.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(15016..15330)
                     /locus_tag="ACG8ER_002053"
     CDS             complement(15016..15330)
                     /locus_tag="ACG8ER_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050625.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT         5284 a         3020 c         2307 g         5875 t
ORIGIN      
        1 aacatctttt gtaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tgtcgtcgag ttgtttgaag aaagcgccga ttttttgttg ttcctcttta
      121 ttcctagtaa aaggaaactt gatattagcg aaattctcat agaataagta ttctcgtacg
      181 cttccttcag tgttgcgctt gacctctttg ataaatgttg gtgacttgat aaactgaagt
      241 atgaactcat tatctaagtc ctcgctcatt ttcacaacta cataaagaga gctaacaatg
      301 accgtcatac caagattgtt aaatgcaatt gatcctacgt ttactcttgc tggattatag
      361 gcgaattcat ttggttcaac gaatttataa tttgtttttt ctaaatcatc caacctacta
      421 ccatcaaact gttctgtttg agaaattaaa cctgctttat tacttaccga ataggcaggg
      481 aagtctaaat ctttcgtgtt cttttttcca gtcttcttac taaactcttt gaacttacgc
      541 tgttcccaat cttccgtaaa tccaggaaac cgaatttctg gcacttttgc tccgttttta
      601 gggaacattt tttgtaagaa tccttttttc gtttccttta ataaatctag cttacgttgt
      661 tgaagagcga tagtgtcgtc cagttgtttg aagaagttcc caattttctt ttgttcttct
      721 aatattggaa attgtactat ccattttttc acttctgaca ttggtacgag atttgtaatt
      781 gctgaacctg gattagctcc tgtgagtttt ctttgcataa aatctgcacg aataaattga
      841 ataaaatatt ctgaatctgt gatttcttta tttattctaa gtctaatcaa agcctgattg
      901 aatacacccg gttttatttg ctttggtact cttgaaattc tcccaattgt tccagcgcca
      961 ctcatgataa aatcgcctgg ttctaattta aatttgatta attcattata ttttttttca
     1021 gtaattttat atcttgtttc gtaattatca taaatagcat tttgttgttc atagacaaca
     1081 aaattgctat tagatacaaa gaattctttc tttaaggcac tcccaaatgg tcccctcctt
     1141 actccatctt ttttatcaag taagcattca aacttacgct gttcccaagc ttccgtaaat
     1201 cctgggaaac gaatttctgg ttgtatatca ttactcatgt ttgaacacct ccagcgcacc
     1261 ttgtaaccac gcttcatttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     1321 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     1381 atcaattggt tcttcttctt caaacgtatc tacatagcgt ggaatattta agttatagtc
     1441 attttctttg atttcatcaa agtttgccaa atgagcgtat ttctcgacat cttttctttc
     1501 agcataggtt tcgaggattt tttgaatgtt ttcttccgat aatttattct gattttttcc
     1561 tttgacaaat tctcgactag cgtcaatgaa taatacgtct cgagtttggc ggttcttttt
     1621 caatacaatc accgtcgttg gaatcgatgt cccgaagaac aagttcgcag gcataccgat
     1681 cacagcataa atactgccgt cttctagtaa tttttgacga atgacccctt ctgcagcgcc
     1741 acggaataaa actccgtgtg gcaagacgat ggccatagtc cctgtttcct tcaaatgata
     1801 gaaaccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt ttccataacg
     1861 attaaaccga gaatcgtcta agaaggtcgt atctgctgac caattagccg aataaggtgg
     1921 gttcattact actgcatcaa atgtgtaagg ttcatctgtt ggccaatcct tattcaacgt
     1981 atccccattg cggagattca tttcttcggc atctacccca tgtaaaatca agttcatttt
     2041 tgctagattg tatgtcgttg tatttaattc ttgcccatga tatttaacgt tatctgggtg
     2101 agttaagtag ttacgcacat ttaacatcaa tgaacctgaa cccatggttg gatcaaaaac
     2161 actaaagaat cgtcgttctt tttgatccaa cgtcacgatt tgtgccatca tatcagaaac
     2221 catatgaggc gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     2281 cagaaattca taagcatccc cgatcacgtc cccatcgtga cctaaaactt ccacgtcatt
     2341 caatttctta atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     2401 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     2461 ttgcacaaag gctttgttta gatcattcaa ttggaacgtt gcttgttttg cttgatccgc
     2521 taagacgttg aaaagatact ctggcgaaat cgcatacccc aaaatatcaa cgattgttgc
     2581 aatcagatct tcttttgatt cttcatctga aagtaattcg cgatacaaca tcgtttgttt
     2641 gcttaccgta tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     2701 acggtcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     2761 atccattttg ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     2821 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     2881 ctccctttta gaaaaagcaa aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     2941 cgaaaggagt ctaacgttgt ttgatataac ttagactgat cgttaactta ttttttatat
     3001 ttctacatag ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt
     3061 ttcataaaaa aacagtactg tctagtagta taaaatatac aaatttctta aaaatttttt
     3121 cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt aatgaaacta
     3181 tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac
     3241 ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt
     3301 agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg
     3361 ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt cccattttca
     3421 agtaaccgaa gtggaatatc tttaccaact ttctctgtca ctgcattact gatacctgtt
     3481 agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt
     3541 atcgtgattc ctttatcttc atttacaagt ttccaagtag gtggtatagc tgtgtatgtt
     3601 tctacaaatg aattaagttt ttcattaagt gacttctctt ttatatttcc atctaaaaac
     3661 tctgtatata tggcttgagc tatactaaaa caaagctggc cttgttctaa taattttaca
     3721 tcattagtta gtaaagctgt gttcacgtta tcaatcgctt caagaagagt atctttggtg
     3781 tccctaacag ctttaggtgt gccttctgga atttccctac ttgatacctc gtccatttta
     3841 tcttccataa accccttaaa atcatatctt tctcccaaat ccatagaagt ttttgagaaa
     3901 gctttcattt ctgagtctat ttcttcaata atatttaaca tgtctgatac atattgttta
     3961 ggctgattcg tttttaatct atatgtggta aatatctcaa ttcctccaat tatcaataaa
     4021 attgccaaac aaatccataa taattttttc tttttcattt ttcctcctat acactgctgt
     4081 tataacagtg actaatgtaa agattttccc agcaatcatc agactattat ttacagggaa
     4141 tcaaaaaaac tccttagtaa gcttacacct ttaggagttc ctgtttgaac ggtatgcata
     4201 atacatttaa atcaatttaa cattaacacc tcagtatatg gtatctctac atatacacgg
     4261 tctttttttg gatagtactc gacatttata ttgatagcac ttccattgtc taacgtagct
     4321 ttagcccggt acgtgttatc accattatca tgagtgatcg tcacatcatc acatgtccga
     4381 tttaagttat actgttcttg taaaatctga gttaccaatg gtacagatga cttctctagt
     4441 ccagatgaac ccgtcattct tgaaaaaatt atacctataa tagccaatac aacgatacct
     4501 ataatgacaa acttcttatt catatttact ttattcaact ttttatctcc tatctccaaa
     4561 atgattttct ctcatcagta actcattgac atccccaata gtacaccaaa gatggctcca
     4621 gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttctctcg atataagtaa
     4681 cccataataa cagcaacagc accaaatacc acaggaataa atgctaatga tactaccata
     4741 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
     4801 ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc ggttgtctca
     4861 tctgtttctt gtaatgctcc gcaaaactgg caatacttac tttcatttgt tatttcttta
     4921 ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct tattagctaa
     4981 ctgtttactt tattaaatac atcagaaaac agctcattta ttggtacact taatatctca
     5041 gaaagcattt tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
     5101 cattgctgtg taacattcat ttttttggct aactcagttt gggaaagtcc tttttctttt
     5161 ctgtatcggc tgagattact tacttttttc atgattctcc ccttctgttt tattatatac
     5221 aacaatatga tgtatttcaa ctcataaaat atataaatac ttcaaatgtc attacaaata
     5281 caacaacttg ttgtactcta ctgagaaggg gggtgtatta atgaaacaga agcaatatgc
     5341 aaaggtcatt agagaaaagc gaaaagcact cgggtggaca caaaaagaat tagctaaaaa
     5401 aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag aacctaacct
     5461 cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt ttctagacaa
     5521 tgttgacata gattatgagg aagagttttt ggctctctat cgctctctta gtactgaaga
     5581 tgcaattcga acaattgact atatgaagct attaaaaagg caagaaaatg aacgcaatca
     5641 gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt gtattgagat
     5701 gaaataagat taagagctat ggataacctg tctagtttaa tataattggg tctcttctat
     5761 tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgataaata ggaactaatt
     5821 caactcctga taatatcaat tcttttatta cttcatctat ccctgcccct ttcgctagat
     5881 atactaaaaa actactgctt gctttcacat tgtatagttt cttaatatcc ttagaagctc
     5941 gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttctattaaa gcatttttca
     6001 cgctgttatc cctgacattt cgtaggcgtt cacaaacagt gttaataagt tctttttctg
     6061 tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct tcatctaaaa
     6121 tcaattgttt ttccttgcta agttctgatc gggaattttg aggaaatact cttttagcct
     6181 ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat gtaaaatgaa
     6241 atttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa gttgcattaa
     6301 aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa gtttcctaac gctttctctt
     6361 cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca tctaattttt
     6421 ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct atgtcataag
     6481 ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa atacaagtca
     6541 cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca tagacacgtc
     6601 gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt gattcaagag
     6661 tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga tacaagtcta
     6721 ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa attagcatca
     6781 caactccaat aaaattttca ctgatatcac aattactgga atgacaataa aaaatggaat
     6841 gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga aacagacatc
     6901 ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat ctttcataaa
     6961 tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac tgtttcattt
     7021 cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact agttcatact
     7081 ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca atcggaagct
     7141 cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac ttatatcctc
     7201 caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg ttctttttca
     7261 agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt aatagaaaga
     7321 caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc gcaaacgcag
     7381 aattatgaac taaattgata gataaaacct tttctatgct taagtatcta ctatcatctt
     7441 tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca tccagaataa
     7501 ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc ttgcttggaa
     7561 taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact tctctatagt
     7621 tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc ttctctaata
     7681 atttttcttt atctacataa attggaatat taatcattga tgtttccatt tgtgtttcct
     7741 ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac tctttattac
     7801 tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa actatcaata
     7861 tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc gtattgagaa
     7921 acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg ctgatagtcc
     7981 atatactcta gagtgactcc agaaataatg tcttctagca taggctttag cactgtaatt
     8041 atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat ttgtagagac
     8101 atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat cttcgtattt
     8161 tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa atgattacca
     8221 gttcatcgac atacacataa tacctcgata aaaaaggggt gtcaatagtt ttgtaaaatt
     8281 tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc aatatgataa
     8341 aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa ataaaagcta
     8401 taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac aacggtcaat
     8461 taattgacaa aaagactatc tacgaatggg agaagggaac ctatttacca ataccagagc
     8521 gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt tgtggcaatg
     8581 tagaagaata tattttggga attattcttt atagagatag cattgtacta gatggtataa
     8641 catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca gtacactcta
     8701 atttagatac atggctggat agatattcca aattagaacc agagatgcaa gaatttattg
     8761 ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat atcttaaaaa
     8821 tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt cttttcctca
     8881 ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct attcaattga
     8941 aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata aacaagctag
     9001 aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag ggaggtgata
     9061 ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttcatgg caaaaatatc
     9121 aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca ttgccaccga
     9181 tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca aacaagaagc
     9241 tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa agaaagcacc
     9301 tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta ccagtgttaa
     9361 agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc cagcatttgg
     9421 taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag atgaatggca
     9481 cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt tacgctacgg
     9541 ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc cacgaaaaaa
     9601 agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt tactggatgc
     9661 ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg catacacagg
     9721 tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt ttaataaaga
     9781 actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa ttcaatctcc
     9841 gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt ctatcctcaa
     9901 caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata ctagctctaa
     9961 agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca ctgttaatga
    10021 ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc atgggtttag
    10081 acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag ttcaacaacg
    10141 attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta caaataatgc
    10201 acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa agagctggtc
    10261 aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct tatatatcaa
    10321 gggttctagc tcctataaca ggtttaccca acactgcctt ccaatgactc ttttgggaat
    10381 cagaggaaag aatcaggctt tatagagata tttcatctaa caaattactt tagactgaca
    10441 gtatatatca gcaggtactg tcatcgggaa cgtagccaat atcgtatcca aaacagcagg
    10501 tagagaagct atttgaggat tcaagttatt ctatttcaac catgaagtta agttcattaa
    10561 aaataaagat agttggtatt tcaataattt ttgtatagag ttaatcactt tattgatgct
    10621 aattcatctt gattggtatt ataatcgtat tcaagttgcg tggcgtgctt ttgctagaaa
    10681 atatataact gttctttata aagaaatata aggattaaac attttttatc atagagtttt
    10741 acttatctat agaattatgt ctgttcctaa ttcagtttga tgaattaact gataaaaaaa
    10801 tatctactat ttaaagatac agggataagt aagtaaaaaa ataacatgtc attagatagt
    10861 agacagttcc aactctttat ttaccttttt tgtatgctgc actgtcaatc caagttccta
    10921 aattgttgta tccatcatca cttatataat catattggcc atcaataatt tcgtcaaacc
    10981 acaaatattc tccatctggt ttttgcccaa ttattttaat tactgattga ctagtagttg
    11041 tcatctggtt aaaatctttt attttaccta tattaacggc caaaatagca tttcctcgct
    11101 tataactctc ctggatttca tatttaacat attctcgatc aagagtttct ttacctatca
    11161 gcaccacagt cactgatgtt ccaattaatt gattatctat ccagtttttt atagcaatat
    11221 ctccattacg tttaactttc tcaaattcag ccttatctac aaaaccagct tcctttgttc
    11281 ctcgcaaagt ccagctgttt ctaactacca ttgctcttgt tacatctgca taatggaaac
    11341 taaaaaaaac ccttctcacc atattaatcc tcctgaatac tatttattaa actgacaatt
    11401 tgttcaactt ttttatctac ttctccttca gtatctaatt tttctagcgc ttgaaattta
    11461 ctttttaagg aatcatcaaa ataatcactg aaattctctt gaatcttatc aaacaaaacc
    11521 ttacttgcac ctttggtaaa tccaatcgga actactatat tttcattttg aacagataat
    11581 tcaaattctt caatcattcc atctgctgta actatttcac ctgattcaag tttgtttcca
    11641 aaaacaaaaa tactaatccc agactctgca ataatgtttt ctcgatatct tctccactca
    11701 tgaggcatat caaggggaag tggtcttaaa gaaagtacgt cttgtatgtt attttttctt
    11761 aaatttcttc tctgctttag aactcctgaa acaatactat taccaattcc aagtccgtaa
    11821 cctgatacta ttctataacc ggattcagaa agcctatatg ctaatttctc gacaaatttt
    11881 aatcctttcg attcagatgt aaatggagta taatcaacag cacttcccga aataaaaata
    11941 gtttttctca aatacttttt tctaatactt ttcaaaatat cagtaatctc agaataatca
    12001 tctacttcaa caacgtttat cgaaaatctg gacaaatcat tgatttgtag tcttcttttt
    12061 gtcagttcat aattatatct ctcttgatca aaaaaatcct cagaatcgac aaaattttcc
    12121 tttaatattt ttctcattat acaataatga ggtctttggt tttctctcaa aacccatctt
    12181 atatctgata aaattctttc taaattaggg tctgtaaaac taaatcctaa aaagagaaaa
    12241 gtttttgtta acaaatctcc ttttaaagtt tctttaaata acgcgtacga atcatcatca
    12301 tatttttcat aatcatctct tgttattact gtcgaactga catcatctat atcaccatgc
    12361 attttataaa caattgtatc cacatttgga ttagttgttg ctaactgagt ataatttttc
    12421 ttaacctgag ggtttttata agcttctcgg attgattttt ctattagatg atcataatta
    12481 gttgtccaat aagtttcaat aggtaaactt gctagtattc gatgattttc agatatttct
    12541 aattcttcac caaaattatc cataagtatc tgattcaaaa ttcctcttga tccaccattt
    12601 tcatttttat aatattgagc cagagtaact aagtctgttt cttttttaga atccaacccg
    12661 atttctgttg caggatcatt aagtagttcc ggccaactta catatccttg tgacatcgat
    12721 aatcctgcac ctgcaaatat tgctgcattt cctttttcaa tagcttcaga gaacctcttt
    12781 attaaaactt tcttatccat tctacccggt tcctttcgat tcagtaaatt gattagagat
    12841 tagtgaaaat ggtgttttta tataaaatat ctaatgtatt acttccttta gtgcattcca
    12901 cagcatctaa agcccatttt ttcaaatcag cttttcgtaa ataagttaat tcacttaaat
    12961 ttaaagaata ttttatttgc agattttctt gcgaagaata gtgctttata atgcttctaa
    13021 catcctcggt gctcatttga ggattacttt ccaatcgtgg attctttttt tccactattg
    13081 aagctgtcgt cagttcgcta tatagccatg gcgaatacgt aaatttctgt tcattagtga
    13141 tttcattgct caaactaatt gaattaggtg tattaagaaa aaacagacat tctgtactat
    13201 caatcatatt atttaaagct gtagaaagca tcatatgaac atgactagtt gttacatttc
    13261 ttttatcata atcatatgtt tttgtttgtt ccttatagca atatttttgg tctatttttt
    13321 ttaataaatc agaactgtat ccccaaaccg aagaatcaat aaatgcagta agaccaaaat
    13381 tttcaaatag ccatgatgca aaacgtatag ctaatttttc atcagcatga gagtgagata
    13441 taaaaatatc tgcttttata gttggaaacc aatctttaat aatttcgtct gcatctagaa
    13501 ttttgctgtc cttagaataa taatcaacta aatcattttc actttcaaat agattttcta
    13561 ttctcttgtt aatagatttt tggttgtgat ctatttgatt tactacatgt gtatatgatt
    13621 gcgagcttga ggagaatgca tttaagtcag catttgtaaa atccaattca tatgccttat
    13681 acaaggatat ccctccattc ttgtattttg atccacaatt tattaggcaa cataacagta
    13741 taccctatcg ttacatatcc cattcattat tataccagta tttcttcgtg acacataagg
    13801 gtgtaacata ataatttcaa acgaaatgac tacaagtagt cattgaagca aatcttcata
    13861 agacacctgt agaatatctt tgatggcttt caattgatcc aacttaatgt gttggcggcc
    13921 accttcaatc ttaaccaacg tttccctagt tatagcaatt ttacgaagct gtaggtctct
    13981 aatcagctcg gtttgaccaa ttcctttttc taatcggact cgtctaatat tgctaccgat
    14041 tacattttct ttatcaataa ttttttcctc tttcaagaat agcacacctt ttggactaaa
    14101 attagtcctt tttcttatta atcttaaaga ttcggtatgc tacaatggga ctagtattag
    14161 tccatattca attttaagga ggaatttttt tgaagaaatt tgcaatcatt ctaacttgcg
    14221 ctttactcat cggaactata agcgggtgta ccacttctga cacaagttct acaagtacgg
    14281 aaactagtac aacaacctca gaaaagaatt acccaataca tttttttccg gttccagatg
    14341 gatttgaagc ttattatcat gataaaaaac tagatgttcc tttatcagaa actgaatcta
    14401 ctgaaaagat tttgccaatt gaaattgaaa gaaatcaaga cgatataaaa ctaaaagctg
    14461 atcttccctt actgaacagc acacaaattg atagacatga aaatattaaa gttaatattg
    14521 aaaaaggagc gttagcacaa ctttatactg aaaaagttga acaatttttt gaggctggtg
    14581 ccaataaaga cttttcaaaa ataagcgatt attcggataa cttttttaat gagacccaag
    14641 atgaaatcac gaactcaact tttatggctg aagatatgta caatatgaag ttgaaaaaaa
    14701 taaagtcgta tgattatcca ttaaatctaa cattaaataa agggatactc tcttttgtat
    14761 tagcaggtta cgttgactat gaatatacag cagattattt ggagacgccc cttcaacacg
    14821 ataataacga tattaatttt agatttatct acaattcaaa aagtaaaaca tggttagttg
    14881 atcaattgag cactataaat acgggatttc atgaattacc taagcctgac aatgaaactg
    14941 ctttggtcat taaaacattc taaaaaagaa acgttgaagg aataattatt ctttcaacgt
    15001 ttcttttatt gtttttcagt ttacggtctt acctgagcga ataatctgat ttctaaatga
    15061 ttggaaaact tcttgtggtt gataataatc tgtatctatt tcaaatgttt ctgagaataa
    15121 ttcattgaat ttttctactg aaatgatgat tttaccattc tcctctcgac tgttttctat
    15181 tatttctgcc tctaattcaa ataaatctag tgacgaacta aaatcataaa tcctgtcata
    15241 ttgaacaaaa aaatcaagaa aaagttgctc aaactcttcc ttatttatca ctagtgtttc
    15301 aagcggaaca actttatttt catcagccat tcttccaatt tcctcacttt cattttcaat
    15361 tgtccattgt tgcggacgta tcagattttc tctatcattt ttcagtgtat agatcacttg
    15421 taatacacaa caatgctttg taaacttatt tttttagtta gtcgaatctt tttaaatgtg
    15481 cgtttcatac catgcgcttg atatattttc tcctattttt taaatcgttt atcacaactt
    15541 ttatcgtttt ttcttctgaa aaaaatcttg tttacatcgt tacatagttt ccaaaaagat
    15601 acattactag tagaaacaac aagattaatt ttacttccca ctgaaatggg aagtatattt
    15661 taaatatttt ttctccttca acttacacta taaagtatct taatcacacg atatgctagc
    15721 cactaaattc atcatatttg attccttttt taagataatg tagcatcttt ttatccaata
    15781 acgcaattgt ttttaaggat aaatttctta agatggtaca tttcaaaatg gcatttgaca
    15841 tatgaaacaa aaaagaaaga aaagagcata agggaaaact gatagcacct tcctacagga
    15901 aggtggctaa gttggctgtg ccaactggtt ttctttcaaa atcatttcat aatttttgat
    15961 atcataaaaa aatccatttt ggacctattt tcggtcataa tttagtacct acttttggtc
    16021 atagtttcgt ccgtagtttt ggtcatagtg actaaggctt ttctgttcac aaaattggtt
    16081 taaatttgct ttctttaaca cttcatttat atcatagcaa ttataatgtt tacacaacat
    16141 cattcctaat tttaacggtt gttcctcttt atatttttta agttctacat ttttttgcgt
    16201 ttaattactt ttctcttttc tttatattcc gctccaactt tctctttaat agtgataata
    16261 gaattacata tttttgttaa acttccttaa aaaaattgga tctcgattac tcaaatttgc
    16321 ttgcaggctt ttctaattgc tttgtttttt cttcctaatt atttgatttt atttccattt
    16381 tcttctagtg aaaatttatt atttttaata atcgtacgcg tagaactgat ttcatttttt
    16441 gtatctgttt gattatctaa cgtttgaaat tcaaagtttt gattgg
//
LOCUS       DAZUMO010000061        15946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000061 DAZUMO010000000
VERSION     DAZUMO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.061"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..526
                     /locus_tag="ACG8ER_002054"
     CDS             8..526
                     /locus_tag="ACG8ER_002054"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5050626.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            654..1337
                     /locus_tag="ACG8ER_002055"
     CDS             654..1337
                     /locus_tag="ACG8ER_002055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS5050627.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1646..1719
                     /locus_tag="ACG8ER_002056"
     tRNA            1646..1719
                     /locus_tag="ACG8ER_002056"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1680..1682,aa:Arg,seq:tct)
     gene            1878..2237
                     /locus_tag="ACG8ER_002057"
     CDS             1878..2237
                     /locus_tag="ACG8ER_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS5050628.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2360..3991
                     /locus_tag="ACG8ER_002058"
     CDS             2360..3991
                     /locus_tag="ACG8ER_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS5050629.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4053..4694)
                     /locus_tag="ACG8ER_002059"
     CDS             complement(4053..4694)
                     /locus_tag="ACG8ER_002059"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5050630.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4880..4956
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4976..5284
                     /gene="rplU"
                     /locus_tag="ACG8ER_002060"
     CDS             4976..5284
                     /gene="rplU"
                     /locus_tag="ACG8ER_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS5050631.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5311..5655
                     /locus_tag="ACG8ER_002061"
     CDS             5311..5655
                     /locus_tag="ACG8ER_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS5050632.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5668..5961
                     /gene="rpmA"
                     /locus_tag="ACG8ER_002062"
     CDS             5668..5961
                     /gene="rpmA"
                     /locus_tag="ACG8ER_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS5050633.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6395..9202
                     /locus_tag="ACG8ER_002063"
     CDS             6395..9202
                     /locus_tag="ACG8ER_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5050634.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9393..9884
                     /locus_tag="ACG8ER_002064"
     CDS             9393..9884
                     /locus_tag="ACG8ER_002064"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5050635.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9987..10976
                     /locus_tag="ACG8ER_002065"
     CDS             9987..10976
                     /locus_tag="ACG8ER_002065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5050636.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11009..11812
                     /locus_tag="ACG8ER_002066"
     CDS             11009..11812
                     /locus_tag="ACG8ER_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS5050637.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11833..12747
                     /locus_tag="ACG8ER_002067"
     CDS             11833..12747
                     /locus_tag="ACG8ER_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5050638.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12830..13204
                     /locus_tag="ACG8ER_002068"
     CDS             12830..13204
                     /locus_tag="ACG8ER_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS5050639.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13343..13588
                     /locus_tag="ACG8ER_002069"
     CDS             13343..13588
                     /locus_tag="ACG8ER_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5050640.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13760..14824
                     /locus_tag="ACG8ER_002070"
     CDS             13760..14824
                     /locus_tag="ACG8ER_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS5050641.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15070..15495
                     /locus_tag="ACG8ER_002071"
     CDS             15070..15495
                     /locus_tag="ACG8ER_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5050642.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15488..15937
                     /gene="nusB"
                     /locus_tag="ACG8ER_002072"
     CDS             15488..15937
                     /gene="nusB"
                     /locus_tag="ACG8ER_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS5050643.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
BASE COUNT         5100 a         2642 c         3482 g         4722 t
ORIGIN      
        1 gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt tgattatatt
       61 tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat
      121 tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc
      181 gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa
      241 aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttatacaaa
      301 agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata ttatcggtag
      361 tcccatcttt cagattccat atattggact agtcctttca aaaacggcat ttgcatggat
      421 tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat ggaatacctt
      481 tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat gttaaaaaca
      541 taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa aacactcatg
      601 cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt agtatgcgca
      661 atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg cgtacccagc
      721 aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta gcagaagtac
      781 cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag cttcagcctg
      841 aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg gccacgacaa
      901 tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc gagaaagcta
      961 aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa ggtcaagcag
     1021 cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat agtgcaaaat
     1081 caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt ggcgttttga
     1141 tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag attccccgtg
     1201 gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg gatcatcccg
     1261 atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca aaggaatcag
     1321 aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt gaatcgaggg
     1381 ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag gatacaggaa
     1441 atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat tgataacctt
     1501 agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg ggccaaaata
     1561 attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt aattatctat
     1621 aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag catccgcctt
     1681 ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg ttagaaactg
     1741 aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc aaaggcaaat
     1801 aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata aaactcatca
     1861 gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta gttgcaattg
     1921 aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg acaagtaggg
     1981 tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg tttaagaacc
     2041 aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat ctttcttccg
     2101 catctaaaga aatcacaagt atgctattaa tatctatttt ttttgttgca ttatatggca
     2161 gtttgactag cgatcgttgg attttactca aacaaggtgg gctagctctg ttggcattgc
     2221 tttctatttt cttataaata tagaaaaaat aagaatttat ctaacaggaa actttgtgaa
     2281 aaatattact ttatctcatg aaatatgata ttctaatgaa tggtatttta gaaaatttca
     2341 tgaaaaagga gttagatagg tgttcaaaaa ttatgtttcc tcactaaaaa aagaattttc
     2401 aggttatgat ctttcaagat ttcagaaaga tcttttggct gggatcacag tagctgctgt
     2461 agcattgcct ttagccttgg cttttggggt atcaagcgga gctgatgcgg ccgcaggttt
     2521 gattacggct atcattgccg gtatcgttat tggcggttta tcaggcggtt tttatcaaat
     2581 atcaggtccg acaggtgcga tggcagctat cttgatgtct attgcggcaa caaagggaat
     2641 gcaaggtgta ttgcttgcta cttttttagc gggtctattt ttacttgcag caggactttt
     2701 ccgattagga acactgactt cttttatccc agcacccgta attacggggt ttacttcagg
     2761 tattgctatc attattgcat taggtcagat cgataattta tttggtgttc actcggaagg
     2821 tacaaacgtt attgaaaaac taagtagtta tcaaacgata ggatttccga taaacacagc
     2881 ctctttgatc atgggagggc tagtcatttt agggatgttt atctatccaa aaaaatgggc
     2941 gaaacgaatg cctagttcgc tgctggctat cattttggca acagcagtta tgctattgtt
     3001 ccatttacca gtagcaactg tgggtaagat tccacaaaca ttgatcagta gtaatcgttt
     3061 gaatatggga gattttagct tttctgcctt gcaacaagtg gcagttccag ctattagcat
     3121 tgctttatta ggaatgattg aaagcttgct atgtggtgct tcggctggcc gaatggcaaa
     3181 taagccctta gatagcaatc aagaattaat ggcgcaagga attggaaatc tgttgcttcc
     3241 tttctttggc gggattcctg ctactgcggc catcgcccgt acaagcgtgg ccatcaaatc
     3301 tggtgcacaa actagattag ctggtatgat acatgcagtt gtcttactgc tctccatgct
     3361 tgtttttgcg ccagtgatgt cacagattcc aatgccagca cttgcaggtg ttttgattgt
     3421 gacggcttgg cgtatgaatg aatgggaaac gatcaaggag ctatttacta ataaatattg
     3481 gtcagcagtt ctattgttta tccttacgat gatttgtaca gttatctttg atttaagtat
     3541 tgctatcgtg atcggggtga tcggcggctg tgtatttttt gttgtcaaaa gcgctgctat
     3601 cacgatttca ttagaaacga tcgattggca aagaatgggg ctgccagaaa cggaaaagct
     3661 gaataactgg acagtagtgt atattagtgg tccactgttt tttatgtctt cagaaaaact
     3721 caaaaataaa ttggaagaac taaatcatca cgatggtgtc attttctcca tgcgaggcgt
     3781 accaagtatt gatttgacgg cacgtgcagt ttttgatgaa tttcaagaaa aagcagagca
     3841 aagaggacaa acgattatct atacttcctt gcagccagaa gtagaaaaac aactttctca
     3901 cttgtgggaa catacattca cgcatcaaca tcaaacagtt gctcatgcgt tgacagcttt
     3961 acatgcgaaa tatactgcaa ttagccatta aaacaatgaa aaagaacaga caagactgta
     4021 aactgaagat tttatggtct tgtctgttct ttttaatatt gctgtaaaag tgaagctagt
     4081 acttgccagt cttgatggat aacaggtaca agggagcgat tgtagaaaat agctgctgga
     4141 tgaaaaagtg gaagaattcg atagtttttt tctgaccatt cgtaggcatc cttttgttct
     4201 gttaattgta agacaggccc tgtatagagt tgaccatgat ttttggtaac cgtatattct
     4261 ttgccaagta aacgttgaag accaatgttt ccaagtgtcg caattaattt tggctggacc
     4321 ttcatcagtt cgtaatccaa tattggtgca tgcgcaaata cttcggaacg agtaggtgtc
     4381 cgatttggat aaacagtttc tgactgttgt gtccgtttat tgatccgatg agtcacgcga
     4441 tacgggcggc tccttacagc actggtgata tacacctctt ctcgtgttaa gccagttgaa
     4501 gaaagtgcct gcatcaattc ttttccagct tgaccgctaa aaggaacatg cgtatcaatc
     4561 tctgttttcc ctggggcttc tcccacgagc atcagtttag gatgaagagg tccttgccca
     4621 gcgataaatc cttctacagg acggccgttc attcttaaag aagcttcttg tatcagttct
     4681 tttgggtatt ccatttatgc tcctcctttt tcttttatta taagaataaa agaaacacaa
     4741 tgcgactatt ttatttggta gaactgtgag gatagaaaag cagaagtatt gcttgaattt
     4801 ttgttttact ttttgatttt aaatttagtt gactaacggt tgctgttctg gtaagctagt
     4861 actgttgtaa ttgtggactc cacagctaca accgctcaga acaagtaata agcacagcat
     4921 atgctgtcac ttcggatggc gagtctaagt atatcagaag gaggtgcttt atagcatgta
     4981 cgcaattatt aaaactggtg gtaaacaagt aaaagttgaa gtgggacaag caatctatgt
     5041 tgaaaaactt gacgttgaag caggagaaaa agttgttttt gacgaagtta tcttagtagg
     5101 cggagaatct acaaaagtag gcgctccaac tgttgcaggt gcaactgttg aaggtactgt
     5161 tgaaaaacac ggcaaacaaa agaaagtcgt tactttcaaa tacaaaccta aaaaacattc
     5221 acaccgtaaa caaggtcatc gtcaaccata tacaaaagtg gttatcgacg cgatcaacgc
     5281 ttaatagcgt ttgagaaaga aggcctatat atgatcaaag gaacatttaa gcgaaatgac
     5341 gcaggtcaga tcgtttcatt cactttgacc gggcatgctg atgctggtcc ttatggaagt
     5401 gatatcgttt gtgcgggtgt ttctgcgtta gcgatcagca ctgtcaacgg gattgcctca
     5461 ttagccggtt ttgagccgat tgttgaaatg aatgaagaag aaggcggtta tctttatacc
     5521 gaagtcactt caggaatgac acaagaacaa aacaatattg ctgagattct attagaaaat
     5581 cttttattag gattacaatc gatagaagct gaaaactcag aatatatcca aattaaaaca
     5641 attaatgata aataggaggt gcagaccatg ttgttatcta tgaatctaca attatttgcc
     5701 cacaaaaaag gtggcggttc tacatctaac ggccgtgact cagaatcaaa acgtctaggt
     5761 gctaaacgtg ctgatggaca aacagttaca ggtggatcaa ttctttaccg tcaacgcggt
     5821 acaaaaatct atccaggtgt aaacgtgggt atcggtggag acgacacttt atttgcaaaa
     5881 gttgatggcg tagtacgttt cgaacgtaaa ggccgtaaca aaaaacaagt gtctgtttat
     5941 ccagtagctc aagaagctta attgaatctt atgattaaag aggttgtgac aagacttttg
     6001 tcacagcctt ttttgtttat aaaaataaaa gttgtagtgg aggctacgaa gtttactttg
     6061 tctttttaca cttaatattt ttttgccaat ttggaatcag gatgttttga taactatcgt
     6121 tttcaattga tggaagctat attgtttgct atcagtgaaa taattatatg gtaaacagtg
     6181 ttctttttct tgtttccatt ataaaaatcg tatcgggatt tttcgttctt ttaaattgaa
     6241 catttctatc tatccgcgtg ttatcaacaa aacaaaatca ggtaaatagt tgacgaaaca
     6301 gaaataaata catagtatta tgttttgaag cctaagtaca atagtctata gaaaaggagc
     6361 gataccattg gcaaaacgca ttgaccgaat ttatatgtat gtcaaagaaa aaaccgctca
     6421 tttaacaaag gcagaatatg atcaaggagt aacaacacag gaaattgctg aagtgttagg
     6481 gattcagcga acaaatagca gtaaagatct gaatcagcta gtgcgcgaag gaaaactgat
     6541 aaagacagac ggacgaccag tacgttacat atatcaaaca ctcgttacgc acgtaaagcc
     6601 agttacaaaa catgtagtca gttacaaaga aacgccggtc tctgaagaaa aaaatacact
     6661 ccaaatagat actaaggata tctttgcaaa aatcattgga gctaacggca gcatgaaaaa
     6721 ttcagtggaa caggcaaaag ctgcgatttt gtatccgcca agaggattaa attgtttgat
     6781 tacaggacct accggatcag gaaagactta ttttgcacac gcgatgtatc attttgcaaa
     6841 agcgcatcat gtaatagatg aggaaacgga actgattgtt ttcaattgtg ctgattatgc
     6901 caataaccct gagcttttga tgagtcattt atttgggtac gcaaaaggtg cttttacagg
     6961 cgcagatgaa gaaaaaatgg ggattatcga tcaagcggat ggcggtatgc tgtttttgga
     7021 tgagattcac cggttgccgc ctgaaggtca agaaatgatc ttttacttta tggatcatgg
     7081 aacttacagt cgtcttgggg aaacaacgaa atcccatgaa gcaaatgtac ggatcgtagg
     7141 tgcgaccaca gaagacccag gttcttctct attagaaact tttgttcggc gtatcccaat
     7201 caatatcaaa cttccggctt ttgaaaaacg tcctgcaaat gagaaagtcg atttagtaaa
     7261 aatcatgatt gcccacgaag caaaccgcac gcagcgaaag atttctttga cagaagatgt
     7321 agcaaaagca ttgatcggca gtgtgaccta tggaaatata ggacaattga agtcaaatat
     7381 ccagttagta tgtgcacgag gatttctaaa tcatatgcaa tcacccgaaa tctcgatcac
     7441 aatcgatgat ttgacagaag ggattcgtag cggattgatt caattagcaa gcaaccgcag
     7501 tgcaatggca gagctttcta aaatattgga gccaaagata actgtagcac caaatgatac
     7561 gatcatgaaa atccaatcgg actcctatga gttaccttat aatctatatg atatcatcgg
     7621 agataaagca gctttactga agtcggatgg tttggatcaa gaagcaatca atcatttcat
     7681 ttctacagat atcaacatcc atctgaagtc tttctataaa gaccatggtt tttcttttaa
     7741 tgcagacagc aaattagcag aatttgtaga tccgaaaatc atagaagtga ccaatcagat
     7801 ttatgtaatg gtcaaaagat ctttaccata tgagtttcag caaaacttta tttatgctat
     7861 gagcttgcat atcagttctt ttttgaaaaa aatcaactta ggagaagaac ggcatacgaa
     7921 tgataacatc cgtgagatgg cttttgattt tccagatgaa tatgctgttg cgaaagaagt
     7981 gcgtagttat atcgaaacgt attttcaagt ggagattcca gaaagtgaag actattactt
     8041 gaccgttcta ctcgtctcgt tacgggcaaa tcaagcaagc ggcagaatcg gtgtagtcgt
     8101 tgcagctcac ggaaacagca cagcaagcag tatggtccaa gttgtccaac agttgttaga
     8161 tgcagagaac gtacgagcag tagacatgcc tttggatatg gatccgaaaa cagcattgga
     8221 ccgaattgaa cgcaatgtac aagaagtaga tgaaggaagc ggggtcatac tcttagtaga
     8281 tatggggtcc cttgcgtcgt ttaattcgca aattcaaaga gacactggaa ttccagtacg
     8341 tactgttgat atggtaacaa cttctctagt tttggaaact gtacgaaagg tctcagtatt
     8401 agggacagat ctagatatgt tgcatgaatc attgaaaaat tttcgaggtt acgcagaaat
     8461 atcttctgta gaatctgttc aggaagcaaa acctattcat aagaaaaaag ctgttttggc
     8521 tatctgcgca tctggcgaag gtacagcaca aagaatcaaa gaattgatcg aacgagcggt
     8581 aaacaagcgt caagaaacag aattagaagt tctagctttg tcgattgtgg aagtgaaaga
     8641 agaactgcca gttatccaaa aagaatatca gatcatagct actactggga ttactgattt
     8701 gaatatccaa gccccattta ttcctttaga acgttttatt gatcaaaata tcgaagtcat
     8761 tttggatcag ttgctgatgg aatcagaatt ggaggaaact gaattcatct ccctagatga
     8821 agagtcagct aaaaatacgt gtgttgagtt tatctctgat aacttcatct ttattaatgg
     8881 tagtaagtta atcgatccaa tgtggcaatt ttctacacaa atctctcaga cgacgggaat
     8941 cggcgatgaa gaatatggat tcaaaatcaa cctagtgatg cacacagcag gaatgatcga
     9001 acgtattatt cggaatgaac cattgactgt ggaagaaaac gaattgacaa atacaacgaa
     9061 tgatcctctt tatagccaat tggcagcctc agttgttctg ttggaagatc agataaaagt
     9121 aaaagtgccg atagaagaaa tgtattatct gctacgtcta gtccataatc agttagataa
     9181 aaaagaatac actgtgccat gaatacacaa aaacgataca cttttatagt gtatcgtttt
     9241 tgtgtattgg ttaagaatcc tttaaattca attagtgtat cgtaaaaaag ttggcacgct
     9301 ttttgcttat agtagtgtgt gctaggaggt ggttgttcac acagaaatga taggggtttc
     9361 tgtgaagtag tgaggaagaa ggagttttgc agatggatat tcgattggca cgtatagatg
     9421 atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa gcaaacaggt gttgaacgta
     9481 tcattgtgat cagcgatgta gtcgctcaag ataaattgag aaagtttcta ctaaaagaag
     9541 ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca gaaaatgatt caaatctatc
     9601 atagtggatt attatcacga gcaaaagtga tgcttttatt caccaatccc caagatgttg
     9661 aagcggttgt ccgctgcggt gtacagttga agtcgttgaa tatcggcggt atgagtttta
     9721 ctgaaggcaa atcaatgatt accaattttg tttcagtaaa tcaacaagat attgattcct
     9781 ttcgttactt gaacagtcaa gaagtggagt tagagattcg aaaagtacct gctgacagga
     9841 aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt gtagcaagaa tggttgttgg
     9901 aaaaggtcaa ataattctgt tgtaatggtt agcaattatt aatatttttg aatttgttac
     9961 aacaaaagta aataggaggt ataaatatgg tagggattat cctagcaagt catggacaat
    10021 ttgctgaagg aatcttacaa tccggttcaa tgatctttgg cgaacaagaa aacgtcaaag
    10081 ccgtgatttt gaaaccaagt gaaggtccag atgacttgag agccaaattg gaagaagcgg
    10141 ttgcttcttt tgataatcaa gacgaagtgt tattcttagt tgatttatgg ggcggaacac
    10201 cgttcaatca atcaaacaca ttattcgaag agcacaaaga taaatgggca atcgtcagcg
    10261 gtttgaattt accaatgttg attgaagcct acgcatcacg cttttcaatg gaatctgctc
    10321 atgaaattgc agcacacatc attgaaacag caaaagacgg tgtaaaagta aaaccggaag
    10381 aattagaacc ggcagaagca ccgaaagcag cagttgaaga cgcacaacct aaaggagcac
    10441 ttccagaagg aactgtggtc ggagacggaa aaatcaaata tgtattagca cgtgttgact
    10501 cacgtctgtt acatggacaa gttgcgacag cttggacaaa agcagtgcaa ccaaaccgta
    10561 ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg taagagacta atcgaacaag
    10621 ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag caaaatgatc gaagtagcaa
    10681 aagaccctcg ttttggaaat accaaagcat tgcttttatt tgaaaatcct gaagacgtat
    10741 tgacagctgt tgaaggtggc gtagacatca aagaattaaa tgttgggtca atggcgcatt
    10801 cggtcggtaa agtcgttgta agtaaagtat tgtctatggg acaagaagac gttgaagcgt
    10861 ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg taaagtacct aatgattcac
    10921 gcgataacat ggatgatatt ttgaaaaaag caaaagctga attagcaaaa gcgtaataaa
    10981 taatattaaa atttatagga ggcttatcat gtctattatt tcaataattt tagtcgtatt
    11041 agttgccttt ctagctggta tggaaggtat tcttgatgaa tttcaatttc atcaaccact
    11101 agtggcatgt acattgatcg gtttagttac cggtaacctc gaagctggta tcgtacttgg
    11161 cggtacgctt cagatgatcg ctttaggttg ggcaaatatc ggtgccgctg tagcaccaga
    11221 tgctgcatta gcatcagtag catcagcaat tattttagtt ttaggtggac aaggtgttaa
    11281 aggtgttcct tctgcgatcg cgattgccgt tcctttggct gttgcagggt tgttcttaac
    11341 aatgatcgta cgtacaatcg ctgttccaat cgttcatttg atggatgcgg ctgctgaaga
    11401 aggaaacatc cgtaaggttg aaatgtggca catcattgct gtatgtttac aaggggttcg
    11461 tatcgcggtc ccagcagcag cgttgctatt tatcccagca caaactgtgc aatcattcct
    11521 tgaatcaatg ccagcatggt taacagatgg tatggctatc ggtgggggaa tggtcgtagc
    11581 tgtaggttac gctttggtaa tcaacatgat ggctacaaaa gaagtatggc cattcttcgt
    11641 tattggtttc gttgtagctg caatttctca attaacattg attgctctag gtgctttagg
    11701 tgttgcatta gctcttattt acttgaacct ttctaaaatg ggtggttcat caaataatgg
    11761 aggcggaagc aactctggtg accctttagg cgacattttg aatgactatt aatcttgaag
    11821 gaggaaaata aaatggcaga agaaaaaatc aaattatcaa aaaaagatcg tttagctgtt
    11881 gcgtggcgct ctaccttcat tcaaggttca tggaactacg aacgtatgca aaacggtggc
    11941 tgggcatttt ctatgattcc agcaatcaaa aaattataca aaacaaaaga agaacgttct
    12001 gcagcattaa aacgtcactt ggaattcttt aatacacacc catacattgc ttctccaatt
    12061 ttaggggtta cattggctct ggaagaagaa cgtgcgaatg gcgcgcctgt tgatgatgta
    12121 gcgattcaag gggttaaagt aggtatgatg ggtcctttag ctggtgttgg tgacccagtg
    12181 ttctggttta cagtacgtcc aatgctaggt gcactaggtg cttcattagc tatgggagga
    12241 aatatccttg gaccaatcat cttcttctta ggttggaaca ttattcgttg ggcattcatg
    12301 tggtatacac aagaatttgg ctacaaagct ggttctaaaa ttactgatga cctttctggc
    12361 ggattgttac aagacgtaac aaaaggtgct tcaattttag ggatgttcgt attagctgcc
    12421 cttgtacaaa gatgggtatc aatcaaattc ttaccagttg tatcaactgt aaaattagat
    12481 aaaggtgctt acatcgaatg ggataaattg ccttcaggtg gtgaaggaat caagagtgca
    12541 tttgaacaag ttaataatgg cttagccctt tctcctgaaa aggtaacaac attacaagac
    12601 aacttagacc aattgattcc tggtttagct gcattaggat taacattgtt atgtatgtgg
    12661 ttgcttaaga aaaaggtttc tccaatcgtt atcattcttg gtttattcgt agtcggtgtt
    12721 gttggacacg tcatcggact tttataatcc ataaaaatat agctcgggcg ttgtcccgag
    12781 ctttttttat atagtatact gttcataaac aaatatctgg agggagaaaa tggttcaatc
    12841 aatcaataca aaagtagact tggtaattga tgctacttcg tttacgggtc tgacagatta
    12901 cgggcaaatc atgataggtg ataatggttt tgagttttac catgcgcgag atagacgcaa
    12961 atatatccaa attccatggg aagaagtgga ttatgtacta gcatcagtca tgctaaaggg
    13021 aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat ggtacataca cattttcttc
    13081 caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat gtagatgaag atagaatggt
    13141 acaatcattg agtttctttg atgtagtgaa acgtggtatt cgtagtatgt ttaaacgcaa
    13201 gtaaaaaata aagaaaactt ctaagaggag gttttcttta tttttttact ttgcagaaga
    13261 aaatatgaaa tagcaaaacc aattatttgt cccctttaaa tcccatatgc taaaattgat
    13321 tagcaaagaa ggaggttata ttatgagttg gttatggaca ttgattgttg gagccgtgat
    13381 cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg taacgaatat
    13441 cattgctgga ctagtcggtt caagtattgg acaagcgatt ttcggcgaat ggggaccaaa
    13501 agctgcaggt atggcactta ttccatctat tttaggcgca gtgattttgg tagcagttgt
    13561 ctcatttttc tttggaaaac gatcataagt ttttacaaaa tagtaaacta aaaggagttg
    13621 cagcgagact gtgactcctt tttggcttta tttatttaaa ataaaaagaa gttttccgga
    13681 aatttttatt taactgtcgt gtacgaagaa aagtttgcta taatggaaaa aagcgtgcaa
    13741 agaaagaggg agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa aaatgcaaga
    13801 agaaaatttg gattcatttt tggttaccag tccgtacaat cttcgttact taacgaactt
    13861 tacaggtacg acaggattag ctgttattac tttggaaaaa gcatttttta ttactgattt
    13921 tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt gaaattatta aaaatgtcgg
    13981 acctattttt gatgaggtag caaacttagt acgcaaagaa gaactgcact cattagcttt
    14041 tgaggaaaca acagtctcat ttttagaata ttcggtttta gaagaaatca tcgatgcgga
    14101 attagtacct gtttcacaga tgatcgaaga actcagagaa gtgaaagatg aagaagaaat
    14161 cgctattatc gaaaaagcat gtcatattgc ggatatggca tatgatcaca tcctgaaaat
    14221 gatccaaccg ggcatgactg agattgaagt cgcaaatcaa ctagactttt atatgcgttc
    14281 tcttggcgct tcaggtgttt cttttgatac gatcgttgct agcggattgc gttcggctat
    14341 gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt gacttgatca cattggactt
    14401 tggttgctat tacgaagggt acgtttccga tatgacacgt acattcgcta ttggtgatcc
    14461 tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat gctcaattag cagttattga
    14521 tgctgcaaaa ccaggaatga ctggtgtcca attagatgca gttgctagag attatatttc
    14581 aaaacatggt tacggggaag catttggaca ttctacagga catgggatcg gtttagagat
    14641 ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag gaaatgtgat
    14701 cactgatgaa ccagggatct acttaccagg aatcggtgga gtacgtattg aggatgactt
    14761 gttgatcaca gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat tgatcatctt
    14821 atagtttcat caatagaaga agtaagcgta aaaatcagtg ttcacagact ttgttataga
    14881 aaatcactcg atttaatgat acactataga agaactttta agctgtaaga gataataaag
    14941 ttgggtgttt tctccaattc atagcagttt gcagctgatc ggcggcagtt ttcttaccac
    15001 gtgatcacta tgccgcatca tagagaattc ggatgtatct cgtgcttagg caaagggagg
    15061 aataaataaa tggcagatga aaaaaatttg attttaaacg caaaccaaga tttaggagag
    15121 atcgtgatcg ctcctgaagt catcgaagta attatcggga ttgcagcttc taaagtagaa
    15181 ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt aggacgtgca
    15241 gctcacggaa aaggtgttta cttagcaaac gaggaagatg gcttgaaagt agatctttac
    15301 tgttatctag aatatggtgt ttcagttcct aaagtagcaa tggaaatgca ggaacgggtc
    15361 agccagcaag tccttttcat gacggatatc gatttatctg aagtcaatat ccatgtagta
    15421 gctgttgttc ctgaaaaatt agaacaacca acaatcgaag aactttttga agacgaagag
    15481 gaagagaatg aataaagaat tatcaagaca cgagattcgg gaaatggcat tgcaggcttt
    15541 atttccatta gacttcaatg cagacctaac aaaagaagat gcgattttca acgcaatcga
    15601 attagaccac cgcgacatga tcaacgaaga tgaatcagag tttgttcctg tttatcttga
    15661 tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga ttgaaaaaca
    15721 tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg gatttggtga tcttgcgtat
    15781 cgcgattttt gaaatgactt atgtgacaga tgtaccagcg gctgttgctt taaatgaagc
    15841 aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca acggtgtttt
    15901 atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc ctagct
//
LOCUS       DAZUMO010000062        15880 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000062 DAZUMO010000000
VERSION     DAZUMO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.062"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..751
                     /locus_tag="ACG8ER_002073"
     CDS             68..751
                     /locus_tag="ACG8ER_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS5050644.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="ACG8ER_002074"
     CDS             753..1589
                     /locus_tag="ACG8ER_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5050645.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="ACG8ER_002075"
     CDS             1600..3354
                     /locus_tag="ACG8ER_002075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050646.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="ACG8ER_002076"
     CDS             complement(3630..3971)
                     /locus_tag="ACG8ER_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5050647.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="ACG8ER_002077"
     CDS             complement(4039..6210)
                     /locus_tag="ACG8ER_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5050648.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="ACG8ER_002078"
     CDS             6421..7278
                     /locus_tag="ACG8ER_002078"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5050649.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="ACG8ER_002079"
     CDS             complement(7349..8206)
                     /locus_tag="ACG8ER_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5050650.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="ACG8ER_002080"
     CDS             complement(8191..10893)
                     /locus_tag="ACG8ER_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5050651.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="ACG8ER_002081"
     CDS             complement(10906..12195)
                     /locus_tag="ACG8ER_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS5050652.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="ACG8ER_002082"
     CDS             12345..13391
                     /locus_tag="ACG8ER_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5050653.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="ACG8ER_002083"
     CDS             13393..15546
                     /locus_tag="ACG8ER_002083"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS5050654.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4512 a         3310 c         2930 g         5128 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgag tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccatttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccacat ttttgcataa atttggctta
//
LOCUS       DAZUMO010000063        15745 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000063 DAZUMO010000000
VERSION     DAZUMO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.063"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..626
                     /locus_tag="ACG8ER_002084"
     CDS             54..626
                     /locus_tag="ACG8ER_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5050655.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            771..1475
                     /locus_tag="ACG8ER_002085"
     CDS             771..1475
                     /locus_tag="ACG8ER_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5050656.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1703..2041
                     /locus_tag="ACG8ER_002086"
     CDS             1703..2041
                     /locus_tag="ACG8ER_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050657.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFNITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2026..2880
                     /gene="lgt"
                     /locus_tag="ACG8ER_002087"
     CDS             2026..2880
                     /gene="lgt"
                     /locus_tag="ACG8ER_002087"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5050658.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3027..3260
                     /locus_tag="ACG8ER_002088"
     CDS             3027..3260
                     /locus_tag="ACG8ER_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5050659.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3458..5362
                     /locus_tag="ACG8ER_002089"
     CDS             3458..5362
                     /locus_tag="ACG8ER_002089"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5050660.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            5716..6081
                     /locus_tag="ACG8ER_002090"
     CDS             5716..6081
                     /locus_tag="ACG8ER_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFS5050661.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANED
                     ILSAIHVFLKLSENLSMKS"
     gene            6098..6711
                     /locus_tag="ACG8ER_002091"
                     /pseudo
     CDS             6098..6711
                     /locus_tag="ACG8ER_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010620830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
     gene            complement(6823..7143)
                     /locus_tag="ACG8ER_002092"
     CDS             complement(6823..7143)
                     /locus_tag="ACG8ER_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5050662.1"
                     /translation="MNSLSTSPLKNESIQEVSKIFKMISDPTRLSILFLLQKEELSVG
                     AIAHSLSMEQSAISHQLKTLKTSRLVKSKRAGKNMIYSLDDLHIFSILEQVSTHIEEQ
                     EKEK"
     gene            7399..9306
                     /locus_tag="ACG8ER_002093"
     CDS             7399..9306
                     /locus_tag="ACG8ER_002093"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5050663.1"
                     /translation="MKEIQEQHSHENNSHDHGKMPIILYFIGLALSLIALFVNGENNL
                     IQNTLFSIASISAGYHVIILEGIGETIENSKRQHKFTPNSHILMGIAAIGASLIGNFW
                     EGTLLVLIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLIMQDGNTTIVNVSELKVG
                     DQLQVLNGDQVPIDGVIVSGSTSIDESSINGESIPKEKSKGDGVFGSTINGTGTFTME
                     VTKETNDTVFSKILQLVNQNQENQTKASSIIQKFEPKYVKFVLIAIPLFILLAPMLFD
                     LTWSQSIYRGLVLLVAASPCALAAATVSVTLSATSNLAKRGVLSKGSAYLSQLADIQA
                     IAFDKTGTLTKGKPEVTNYYFSDSVNEENMIDIVVALEKESNHPLASAILDKFEPKNK
                     LAIEVENQIGKGLIGDYNENTYRIGKPTSFEAVSDDYTRLNKEWASEGNTVVYVSENE
                     KVIGLIALMDIPSEHAKATIDYFKKCGIHTTLITGDSEMTGQAVGKQLGVDQVIANVM
                     PEDKSNIINEQKETYGITAMVGDGINDAPALVKADVGIAMGEGTDVAVDVSDLVLMKN
                     DLSKLVKAHEISLKMNRVIWQNIIFSMVVVAFLITVSLVGLTDIAISVIVHEGSTLVV
                     ILNGLRLLKSK"
     gene            9400..9621
                     /gene="relB"
                     /locus_tag="ACG8ER_002094"
     CDS             9400..9621
                     /gene="relB"
                     /locus_tag="ACG8ER_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5050664.1"
                     /translation="MTVISLRIDTQEDKLIKEYAKAKNISVSALIRSSVLEKIEDEID
                     LGLYNQAMKDHKENPKSISFEDMLKELSD"
     gene            9622..9894
                     /locus_tag="ACG8ER_002095"
     CDS             9622..9894
                     /locus_tag="ACG8ER_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5050665.1"
                     /translation="MSSGYQVEFEKGAQKALKKMDKYQSLLIMGWIQKNLVNCTDPRQ
                     HGKGLTVNRSGEWRYRIGDYRLIADINDETVTILMLEIGHRKNIYK"
     gene            complement(10025..12991)
                     /locus_tag="ACG8ER_002096"
     CDS             complement(10025..12991)
                     /locus_tag="ACG8ER_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS5050666.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            13137..13712
                     /locus_tag="ACG8ER_002097"
     CDS             13137..13712
                     /locus_tag="ACG8ER_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5050667.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            13926..14621
                     /gene="vanR-A"
                     /locus_tag="ACG8ER_002098"
     CDS             13926..14621
                     /gene="vanR-A"
                     /locus_tag="ACG8ER_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5050668.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            14599..>15745
                     /gene="vanS"
                     /locus_tag="ACG8ER_002099"
     CDS             14599..>15745
                     /gene="vanS"
                     /locus_tag="ACG8ER_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFS5050669.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKR"
BASE COUNT         5059 a         2657 c         3201 g         4828 t
ORIGIN      
        1 aggaatcaaa gaaagctatt ttaaacaaac caaaattata ggaggaataa atcatgactg
       61 aaaataaagc tactcaagaa agaacacccc aagaaagatt gcaagaagaa caagaacaca
      121 aggaacatgt tcatcatacg aaaattaatg cagcggccat tgcggatcat cttttaggca
      181 acatgcatac attacatgtg aaactgcacc aatatcactg gtatgtaaaa ggagccaatt
      241 tctttacgtt gcatgaaaaa tttgaagagt tgtataacga aaatgaaaaa tggttcgaca
      301 aacttgcgga gcgtctaatt acttctgggc ataaacctgc ttcgacaacg gttgaatttg
      361 aaaaatattc gatgctttcg gaagatgcag ttaataaata cgctaaagca gaagacatgg
      421 ttgaaaatat tatcgaggac ttcagaagca ctcgtgacct aactattcgc gcgattcgtt
      481 tagctcaaga cgagggtgac gatgctttag aagatacatt gattgctttt aaaaatgact
      541 tagacaagaa catttggatg ctgcaagcgt tcattggtaa agaagcatta gaagatgatg
      601 acgctcttga tgaggatgaa gattaaggta aaagtagaag ctgatttagg gattggaatt
      661 ctttccagtc ctttttccat tttcttgatg tgcatcaatt tttttgtagt aagagactta
      721 tataataaaa gcagcgaata taaacaaaat aaataaaggg ggagaatgaa ttgactaaac
      781 atacccatca tgaaattggc gaccacgcag cctgtatccg cttggttcca atattcaatc
      841 acttagagga tagcgcgatg aattacattg ctgaaaaagc ccacacgaaa cagtatcaaa
      901 aaggagagtt tctgtttcgt tcacaagaaa aagaggatac tttatatatt atcaatcgtg
      961 ggaaaatccg tatttatcga ttggcagact ctggcaaaga gcagctagtg cggatcttga
     1021 accctggtga tttcaccgga gaatggacct tgttcaaccc ggatgcagta cacgaggaat
     1081 atgccgaagc aacccgagat accgttgtct gtatgattca gcaaaaagat gtccaagatt
     1141 ttctggaaca atacccagct atttctttaa aactattggc ggaaatgtct aaacggttag
     1201 aaagttcaga acgtcaaacg acacaagtag ccgtggagag tgtcattacc cgtttagttt
     1261 tgtttttggc agaaaatgtg gaacctgaaa tgggcaactc tcccaccatc accctgccga
     1321 tggcaaaaaa ggacattgct tcctatttag gaacgacacc tgagaccatc agtcgcaaat
     1381 ttggagaatt ggaggacgag ggattgattc aacagctgtc tgggaaaagg atcaagattc
     1441 aggatttaga tgatttattg ctttacagcg aataatcgga cacacttttt tctacacgtt
     1501 ggtgttctgt cgctcttcat taacaacggg acagaactca gtgcttttgc ttccatctgt
     1561 ccgtttttag cttttttaag acagaacgac cttcattcat tggaaaaata aaagcacaaa
     1621 catgataacc ctcaagtttt tttgttttat ttttctttaa gatagggttg tcgaacaaaa
     1681 cgtaaaaata agtagggtga atgtgcattg tttttgggtg atagttgcca tcacgttgtc
     1741 agcagcgggt gcattctttg tcgtcccttt caccaagaac acgattggcg gcgaagcaaa
     1801 aacgtctaac gttgttgagg cagataggtt agctatggaa aaagaagagg aagttgtaaa
     1861 aggagccggc gtaatcggac acggaaaagt ggcgtattac gaagcgagtg accagggaga
     1921 taaaagtgta ttcactgttt ggtttccatg gagcttagcc ggaatcttgt tcaacatcac
     1981 catgactaga atgacgcgaa aaattgaaga aatggagcaa atagaatggg attcgtatta
     2041 ggcgttttga atccccatgt ggtggagatt ggttctgttg tgattcattg gtatggagtc
     2101 attattgcag cgggcatatt agcggctctc cagttgagca ccaaggaagc aaagaaaaaa
     2161 ggactggaag aggataccat tatcgatatg gccttatggg caattccgat tggactcctc
     2221 ggcgctcggc tttattatgt gttatttgaa cttggttatt atctgcaaaa tccaggacaa
     2281 atccttgcga tttggaatgg cggcattgcg atttatgggg gattgattgc aggagggggt
     2341 acgctatatt ggtatgcaaa gaaaaaaggt acttctttgg cactcgttct tgatatttta
     2401 gcgcccaatg tattgttagc ccagtccatt ggtcgttggg gcaattttat gaatcaggaa
     2461 gctcatggtg ggccggttac tcgtcaattc ttggaaaatc tttatttacc tgaatttatc
     2521 attgaacaaa tgaatatgaa tggtacctac taccatccga cttttttata cgagtcattg
     2581 tggagtttgt tgggctttgt cctcatcgtc actatacgga accgaaaaca gcttttgcgt
     2641 caaggagaag tcgctttgag ttacgttttg tggtactcag tgggtcggtt cttcattgaa
     2701 ggtatgcgaa cggacagttt atggattggc gagtggatcc gcgtttcgca agccttgtct
     2761 ctggccctgt ttattggcgc gattgtagta tggtttatac gcagacaaga ttatccacca
     2821 atttcttatt attctgatgg caataagggg cagaaaaaga ctattaagat ggtgaattga
     2881 gcaaaaagat aaaaagaagt gcaaaacttg atatgcgtca attttcgttt gctaaaagtg
     2941 agctatacta cagttgtaaa gaggaaaagg cagtgttcat taatctgctg ccgataaaat
     3001 aaaaacacat aaaggagata ggaattatgg aaaaagctat attgcaatta gaaacgttgt
     3061 cttgtccaag ttgtatgcaa aaaattgaag gtgccgtgaa atcagttaac ggtattgata
     3121 aagacagcat taaagtatta ttcaactcca gcaaagtcaa aaccaatttt gattcagctg
     3181 tcacttcgat tgaagagatt caaaaagcta tcgaaaacgt aggctatccg gttcttaaag
     3241 caaaagtcaa ggccgtttaa cagattaaat gaactaggca attctggaag aaaagctatt
     3301 tttccaggat tgcttttttt gttgcaatcc gattcgaggt gcttagaaac gataaaagag
     3361 aactttctaa acttgatgtc agtcaattaa ttacttaggc aattatctta tactgtgttt
     3421 atcaataaaa gagaaattaa atttggaggg aaaaagcatg caacaatata tattaagcaa
     3481 gaaaaatgtt atcaccgtca tcagtggatt gttaatcgta ctcggtttct tcagtcactt
     3541 tgttttagaa aacgtaggac tttcagagtg gtctttgatc atcgcctctg tctttgggat
     3601 tacgccaatt gccattcaag cgtttcaagc aatgaaagtc aaagtcatca gtattgatgt
     3661 cttagtcagt attgcagcga ttggtgcgct ttttattcaa aattatgaag aatcggctat
     3721 tgtcacgttc ttattcttat tcggacacta cttggaacaa cgaacattga accaaacgcg
     3781 atctgctatc aaagaattga ctgaaatggc acccgaaagc gccttgaaac aaatggataa
     3841 tggcgaattt gaagaagtag aagtggatga tatagatgaa ggggatatct tgctcgttaa
     3901 aacgggtgcg aaagttccag tagatggcat agtccttacg ggtgaagggc atatcaatga
     3961 agctagcatt acaggtgaag ctattccggt caacaagcta gctgatgcag aagtttttgc
     4021 aggaaccatt ttagaaaacg gaacgattca aatcagagct gaccgagttg gtgaggacac
     4081 cacatttgga aaaattattg aactcgtgga agaagcacaa gattctaaat ccgaagcgga
     4141 acggttcatt gatcgctttt ctaaatacta cacaccagct gtcttggttc tggcaattgt
     4201 tgtatgggcg ttcagtcaaa atattgagtt agcaatcacc atcttggttc taggttgccc
     4261 aggcgcatta gttatcggag tgcctgtctc aaacgttgcc ggtattggga atggtgctcg
     4321 taacggtgtt cttttaaaag ggagtgaagt cattcaagac ttcagtaatg tggatacaat
     4381 tgtatttgat aagacaggta ctttaactgt cggaaaccca accgttgcag cgactgaact
     4441 gtatgaaaaa gatcctgctg aaacgcttgg ctatctggcc agtgtggaac gggaatcaga
     4501 tcatccatta gcaaaagcgg tcttaaatca aattggagaa acaaactttt atcctgttga
     4561 aggaactgaa gttgtgaaag gcggcggaat cgtttcaagt gtagctggac atagagtggc
     4621 tgttgggaat gtggccttga tggaaaaaga aaatgtcact ctcagcaaaa aagtgcaaaa
     4681 agatgtcaaa cggtttgaac aacaagggaa ctctctcgtt ttgacagcgg tcgacggtga
     4741 attaaaagta ctgatgggca ttcgcgatca agtccgtccg ggtgtgaaag ctaatttgca
     4801 ggaattaaaa gacttgggcg tgaaaaatct agtcgttctt tcaggagata accaaggaac
     4861 cgttgatgtg gtcgccggcg aacttggttt gaccgaagct cacggctaca tgttgccgga
     4921 agacaaatcg gcttatatcg gaaaacttca acaacgtggt caaattgtag cctttgttgg
     4981 agatggcgtt aacgatagtc cgtccttagc tttagcagac attggaatcg caatgggaag
     5041 cggaacggac gtagcgattg aaacatccga tgtcgtttta atgaactcga acttcagcaa
     5101 cttgcctcac gcattaggat tagtaaaagc caccgcaaat aatatgaaac aaaatatcgt
     5161 gatttcagtt ggagtagtgt tggtcttgtt gaccagcgtc ttcttcagcg aatggatgaa
     5221 tatgtctatc ggaatgttgg ttcatgaagg tagtatcttg gtggtaattt tcaatggcat
     5281 gagattaatg aagtataagt tgaaaggtcg gaaagaacaa aaagaagtat cagcacctac
     5341 agagaaagta aaaatatctt aagaataaat tagaagaggc tgagacaata gtccatcctt
     5401 gaaatgacaa aagcggaaac tcagattttt aaatctggga ttttccgctt ttttgagttc
     5461 ttgaaaaaaa ataagagtat agagtatgtt ctttataaaa ttaattttcg tgtaaattat
     5521 taaaaattta ataataaaat ttaaattcct attaagttgt aacacatgaa attgcttaaa
     5581 atagtgtatc aaaaaccttg ttttattaat gttgaattta tttgaaaggt ctacagatta
     5641 acatattatt gacaaaacaa tatttaaata gttatacttc attcaaacgt ctgtttgaac
     5701 gaggtggtga agttaatgac tgcagaagta tgtacaacta cttgtataca taaagaaaaa
     5761 gtagctagtg ttaaaaataa tttaaaagat aaagaccttt caactttact tgtattagga
     5821 aaatgtttta gcgattcttc taggattaaa attttctatg ctttagaaac gtataaggag
     5881 atgtgcgttt gtgatttagc agcgatactt aatgctagta ttgctacaac gtcacaccat
     5941 ttacgttttt tgaaaaagaa cggaatggcg aaatcacgtc aatatggaaa ggtagtttat
     6001 tactctttag cgaatgaaga catcctttcc gctattcatg tttttttaaa gttatcagaa
     6061 aatctatcta tgaaatctta gtttggagga agttattttg ttacaaagta ttttttcagc
     6121 tatcgctgtt tatatttcta ccagcattga ttatttgttt atcttgctga ttattttttc
     6181 tcaaagtcat acaaaaaagg gctccgtcag attttttggg gtcaatatct aggaacaggt
     6241 atcttagtag ccgtaagttt atttgcagca tatgtgctga atttcattcc gcaagattgg
     6301 atcattgggc ttctcggtct aattccgatt ttcttaggaa tcagagtggc attggttggc
     6361 gaagaggagg aagaagaaga ggaagtcgtt gaaaagcttg aatcaagagg aacaaaccgc
     6421 ttcttttgga ccgttgcctt aataacaatt gcttctggtg gcgataactt agggatttat
     6481 attccttact ttgcctcgtt atctttttca gagattgtca ccgctctaat cgtatttgct
     6541 atttctgttg cggttctttg ctatatcagt tacaaattag ccaagatttc ttttgtttcc
     6601 gaaaccttag aaaaatatga acgaatcctt gttcctatag tgtttattgg cttaggaatt
     6661 tatattttga tagaaaatgg gacgattcaa acactattaa attttatata aacaactacg
     6721 atagtgaagt tcaaataaaa aaagacagcg agattttaac tcgctgtctt tttttatttg
     6781 aactaatgaa ttgtaaaatc attttaacct tgtagtttgt ctttattttt ctttttcttg
     6841 ttcttcgata tgagttgaaa cttgctcaag aatactaaaa atatgaagat cgtcaaggct
     6901 ataaatcata tttttaccag ctctttttga ttttactaat cttgaagtct ttaaagtttt
     6961 taactggtga gaaattgctg attgttccat actcaaagaa tgcgcaatag ctccaacgct
     7021 aagttcttct ttctgcagaa gaaataaaat tgaaagtcgg gtagggtcac tgatcatttt
     7081 aaatatttta cttacctctt ggatagattc attttttaat ggagaagttg ataatgaatt
     7141 cataagaatt tctctccttc atatgtttgt ttattcacat ttagattatc ataatatttt
     7201 tataaagcaa gattttttgt ataagggtag ctattttaga gtcctatttt ttttatcacc
     7261 taaataagat aactattttt ttgacaaatt agatagagcg atataaaatg aatgtataga
     7321 catatgaatg ttcgttcaaa cgtaattcgt aagcattacg atttctaaaa aaaagtttta
     7381 ttaagaaagg tgatagaaat gaaagagatt caagagcaac actcacacga aaataatagt
     7441 cacgatcatg ggaaaatgcc aattattttg tactttattg gtttagcatt gtcgctcatt
     7501 gcgttatttg taaacggaga aaataattta atccaaaaca ctttgttttc aattgcttca
     7561 attagtgctg gttatcatgt cattattctt gaaggaattg gagaaacgat tgaaaactca
     7621 aaaagacaac acaaatttac ccctaattct catattttaa tgggaatagc tgcaataggt
     7681 gcttctctga taggaaattt ttgggaagga acgttattgg ttcttatttt ctctggagca
     7741 cattttcttg aagattatgc tgaaggaaga agtaaaagag aaattaccaa gttattagaa
     7801 atgaatccaa caacagctag gctaatcatg caagatggca atacaacaat cgttaatgtt
     7861 agtgagttga aagtaggaga tcaacttcaa gtgttaaacg gtgaccaagt accgattgac
     7921 ggagtaatag tatcaggctc tacttcgatt gacgaatctt ctattaatgg tgagagtata
     7981 cctaaagaga aatcaaaggg agacggagtt tttgggagta caattaatgg aacgggtacc
     8041 tttactatgg aagtcacaaa agaaactaac gatactgtct tttcaaagat tttacaacta
     8101 gtgaatcaaa atcaagagaa tcaaacaaaa gcgtctagta ttatccaaaa gtttgagcct
     8161 aagtatgtta aatttgtttt aattgcaatc ccactcttca ttttacttgc tcctatgctg
     8221 tttgatttga catggtcaca aagtatttat agaggattag tccttttagt cgcagcttct
     8281 ccgtgtgctt tagcagcagc taccgtatcc gtaacattgt cagccacttc taatctagca
     8341 aaaagaggcg tactttcaaa aggaagtgcc tatttgtccc aattagcgga tattcaagcc
     8401 attgcctttg ataaaacagg aacattaact aaagggaaac cggaagtaac caattattac
     8461 ttttctgatt ctgtgaacga ggaaaatatg attgatatcg tagtggctct tgaaaaagaa
     8521 tcaaatcacc cgttagcaag tgctatttta gacaagtttg aaccaaaaaa taaactagct
     8581 attgaagtgg aaaatcagat tggaaaaggt ttaataggcg attataacga aaacacttat
     8641 cgtataggta aaccaacttc ttttgaggct gtatcggatg actatacacg tttgaataaa
     8701 gaatgggctt cagaaggtaa tacggttgta tatgtatcag aaaatgaaaa agtaatcggc
     8761 ctgatagcat taatggatat tccaagtgaa catgcgaaag caactataga ttactttaag
     8821 aagtgtggta tccataccac tttaattact ggggattctg aaatgacagg gcaagctgtt
     8881 ggtaaacaat taggagtaga tcaagtaatt gcaaacgtca tgccagaaga taaatctaac
     8941 attatcaatg aacaaaaaga aacctatggt ataaccgcta tggtgggaga tggtataaac
     9001 gacgcacctg ctcttgtgaa ggcagatgtt ggaattgcta tgggagaggg tacggatgtt
     9061 gcagtagatg tatctgatct agtattaatg aaaaatgatt tgtctaaatt ggtaaaagct
     9121 catgaaattt ctttaaaaat gaatcgagtt atttggcaaa atattatttt ctcaatggta
     9181 gttgtcgctt ttctaattac cgtaagctta gtaggattaa cggatatagc cattagtgtg
     9241 attgttcatg aaggaagtac cttagtcgtg atattaaatg gtcttcgatt attaaagtct
     9301 aaataaatac atgaaattgg ataaaatcat gtatctaaag tttgcagcct ctctaatttt
     9361 gtattacaat gtatatacaa aaagaagggg gtgattctaa tgactgtcat atcgctcaga
     9421 attgatactc aggaagataa attaattaaa gaatatgcta aggcgaagaa tatttcggtt
     9481 tcggctctga ttagaagttc tgtcttggaa aaaatcgagg atgaaattga tttgggccta
     9541 tataatcaag ccatgaagga tcataaagag aatccgaaaa gtatttcttt tgaagacatg
     9601 ctaaaggagc tatctgatta aatgagctca ggttatcaag tcgagtttga aaaaggagct
     9661 caaaaagcgt taaaaaaaat ggacaaatac caatcccttt taattatggg atggattcaa
     9721 aagaatctag tgaattgtac cgatcctagg cagcatggaa aaggattaac cgtcaatcga
     9781 tcaggcgaat ggcgctatag aatcggtgat tatcgcttaa tagctgatat caatgacgag
     9841 accgtaacca ttttaatgtt agagattggt catagaaaaa atatttacaa ataaaccatt
     9901 caaattgagt gcaagcatgt ttaataaaaa aggattctac tgctttttaa ggggtagcgt
     9961 caggaaaatg cggatttaca acgctaagcc tattttcctg acgaatccct cgtttttaac
    10021 aacgttaaga aagttttagt ggtcttaaag aatttaatga gactactttc tctgagttaa
    10081 aatggtattc tcctagtaaa ttaatatgtt cccaacctaa gggcgacata tggtgtaaca
    10141 aatcttcatt aaagctacct gtccgttttt tatattcaac tgctgttgtt aggtggagag
    10201 tattccaaat acttatagca ttgataatta tgtttaaagc actggctctt tgcaattgat
    10261 gctgtatggt gcgttctcta agctcacctt gttttccgaa gaaaatagct cttgccaatc
    10321 cattcatggc ttctccttta ttcaatcctc tttgtatttt tcttcttaat gattcatccg
    10381 atatataatt caaaataaag atcgtttttt ctattcggcc catctcacgt aaggctgtag
    10441 ctaagctgtt ttgtcttgaa taggaaccta gcttccccat aataagggat gctgaaactg
    10501 ttccctccct tatagaatga gctaatcgca aaacatcctc ataattttct ttaatgacct
    10561 ttgtatttat ttgtccacgt aaaatggctt ctagttttgg atactcactt gctttatcta
    10621 tcgtaaataa ttttgagtcc gataaatccc ttattcttgg ggcaaattta aatcctaata
    10681 aatgagtcag tccgaatatt tggtcagtgt aaccggcagt gtctgtataa tgttcctcta
    10741 tgtttagatc cgtctcatga tgtaacaaac catccaaaac atgaatcgca tctcttgaat
    10801 tagtatgaat aatctttgtg tagtaagaag agaattgatc acttgtaaat cggtagatgg
    10861 tggctccttt tccagttcca taatgtggat ttgcatctgc atgtagtgat gaaacaccta
    10921 gctgcattct cataccatct gacgaagatg ttgtaccgtc gccccaatag aaaggcaatt
    10981 gtaatttatg atgaaagttt actaatatgg cttgggcttt attcatggca tcttcataca
    11041 tgcgccattg agatacattg gctagttgct tatatgtaag tccgggtgtg gcttcggcca
    11101 tcttgctcaa gccaatattc attcccattc ctaaaagggc agccatgata atgattgttt
    11161 cttccttatc tggttttcga ttattggaag catgagtgaa ttgctcatga aatcctgtta
    11221 tatgggccac atccatgagt aaatcagtta attttattct tggtagcatc tgataaaggc
    11281 ttgcactaaa tttttttgct tcttctggaa catctttttc taagcgtgca agtgatagct
    11341 ttcctttttc aagagaaacc ccatctaact tattggaatt ggcagctaac cactttaacc
    11401 tttcattaaa gctgctggtt ctctccgtta tataatcttc gaatgataaa ctaactgata
    11461 atctcgtatt ccccttcgat tgattccatg tatcttccga aaacaaatat tcctcaaaat
    11521 ccctatattg tctgctgcca acaatggaaa catctcctgc ccgaacatgc tcccgaagtt
    11581 ctgttaaaac agccatttca tagtaatgac gattaattgt tgtaccatca tcctcgtata
    11641 aatgtctttt ccatcgtttt gaaataaaat ccacaggtga gtcatcaggc acttttcgct
    11701 ttccagattc gttcattcct cggataatct caacagcttg taaaagtggc tcatttgcct
    11761 ttgtagaatg aaattccaat actcttaata gcgttggcgt atattttctt agtgaataaa
    11821 accgtttttg cagtaagtct aaataatcat agtcggcagg acgtgcaagt tcctgagcct
    11881 cttctactga agagacaaag gtattccatt caataaccga ttctaaaacc ttaaaaacgt
    11941 ctaatttttc ctctcttgct ttaattaatg cttgtccgat gttcgtaaag tgtataactt
    12001 tctcatttag ctttttaccg ttttgtttct ggatttcctc ttgagcctta cgaccttttg
    12061 ataacaaact aagtatttgc ctatcatgaa tttcaaacgc tttatccgtt agctcctgag
    12121 taagttgtaa taaatagatg gttaatatcg aataacgttt attttcttga aagtcacgga
    12181 atgcatacgg ctcgtatctt gagcctaagc gagacagctg caacaggcgg ttacggtgca
    12241 aatgactaat ttgcactgtt tctaaatcca ttcctcgtat gtattcgagt cgttctatta
    12301 tttttagaaa agtttcgggt gaaggatgac ccggtggctc ttttaaccaa cccaatatcg
    12361 ttttattgga ttcggatgga tgctgcgagg taataatccc ttcaagcttt tctttttgct
    12421 catttgttag agatttacta accgtattaa atagcttctt ttcagccatt gcccttgctt
    12481 cccacaccat tctttcaagt gtagtgatag caggcagtat aattttgttt tttcttagaa
    12541 aatctatgca ttcatgcagt agatgaatgg catcaccatt ttccaaagct aattgatgaa
    12601 ggtacttaaa tgtcattcga tattcactca gggtaaaagt tacaaagtcg tattcacttc
    12661 gaatttcttt caaatgatcc caaagtgtat tttccctttg aggataatga tcaagcgagg
    12721 atggactaac accaatctgt ttcgatatat attgtatgac cgaatctggg atgcttttga
    12781 tatgagtgta tggccaaccg ggataccgaa gaacagctaa ttgaacagca aatcctaaac
    12841 ggttttcttc cctccttcgc ttattaacta tttctaaatc ccgtttggaa aaagtgaagt
    12901 aggtccccag tatccattca tcttcaggga tttgcataaa agcctgtctc tgttccggtg
    12961 taagcaattc tctacctctc gcaattttca ttcagtatca ttccatttct gtattttcaa
    13021 tttattagtt caattatata tcaatagagt gtactctatt gatacaaatg tagtagactg
    13081 ataaaatcat agttaagagc gtctcataag acttgtctca aaaatgaggt gatattttgc
    13141 ggaaaatcgg ttatattcgt gtcagttcga ctaaccagaa tccttcaaga caatttcagc
    13201 agttgaacga gatcggaatg gatattatat atgaagagaa agtttcagga gcaacaaagg
    13261 atcgcgagca acttcaaaaa gtgttagacg atttacagga agatgacatc atttatgtta
    13321 cagacttaac tcgaatcact cgtagtacac aagatctatt tgaattaatc gataacatac
    13381 gagataaaaa ggcaagttta aaatcactaa aagatacatg gcttgattta tcagaagata
    13441 atccatacag ccaattctta attactgtaa tggctggtgt taaccaatta gagcgagatc
    13501 ttattcggat gagacaacgt gaagggattg aattggctaa gaaagaagga aagtttaaag
    13561 gtcgattaaa gaagtatcat aaaaatcacg caggaatgaa ttatgcggta aagctatata
    13621 aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat
    13681 tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata
    13741 tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc
    13801 cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc
    13861 ttataaattc ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga
    13921 taactatgag cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg
    13981 aattatactt aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat
    14041 tggaatgtat agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca
    14101 caagcggcct tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc
    14161 tgaccgggaa agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt
    14221 atataacgaa gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc
    14281 gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc
    14341 ttgtcattaa tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc
    14401 ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg
    14461 agctgctatt tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca
    14521 ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata
    14581 taaaaacggt atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa
    14641 ctagaacgaa aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg
    14701 ttgtatattc gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa
    14761 aacaaatatg acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac
    14821 aatatagata tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc
    14881 atgctttcaa aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt
    14941 cagaacgaag ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc
    15001 aacacattaa aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga
    15061 aaaaatgacg ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc
    15121 ggttatttga gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat
    15181 gtgcatatca cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag
    15241 attacacggt ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat
    15301 atgctggtgc agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg
    15361 gttattcacg cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc
    15421 tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt
    15481 accgcgggcc tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca
    15541 aaagataagc tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc
    15601 gatacgggtg gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga
    15661 gggcagattt acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg
    15721 atgccagact tggttgataa aagga
//
LOCUS       DAZUMO010000064        15513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000064 DAZUMO010000000
VERSION     DAZUMO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.064"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACG8ER_002100"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACG8ER_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACG8ER_002101"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACG8ER_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACG8ER_002102"
     CDS             complement(1147..1716)
                     /locus_tag="ACG8ER_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5050670.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="ACG8ER_002103"
     CDS             complement(1790..3079)
                     /locus_tag="ACG8ER_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050671.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3406..4281
                     /locus_tag="ACG8ER_002104"
     CDS             3406..4281
                     /locus_tag="ACG8ER_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5050672.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4295..4618
                     /locus_tag="ACG8ER_002105"
     CDS             4295..4618
                     /locus_tag="ACG8ER_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050673.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5367
                     /locus_tag="ACG8ER_002106"
     CDS             4615..5367
                     /locus_tag="ACG8ER_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS5050674.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="ACG8ER_002107"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="ACG8ER_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACG8ER_002108"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACG8ER_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS5050675.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHVGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="ACG8ER_002109"
     CDS             complement(9831..12017)
                     /locus_tag="ACG8ER_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5050676.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACG8ER_002110"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACG8ER_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS5050677.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQFEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="ACG8ER_002111"
     CDS             complement(12239..12679)
                     /locus_tag="ACG8ER_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS5050678.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13292)
                     /locus_tag="ACG8ER_002112"
     CDS             complement(12753..13292)
                     /locus_tag="ACG8ER_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFS5050679.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13907..15373)
                     /locus_tag="ACG8ER_002113"
     CDS             complement(13907..15373)
                     /locus_tag="ACG8ER_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5050680.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4414 a         3243 c         2750 g         5106 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgac gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc gaattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
    12901 aaagataaaa attctaagta ttccctgttt agtttctctg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
    13081 gtagaagtat aatttccttc aacaatccat gacggattag ataccataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
    13201 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
    13261 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
    13321 ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt
    13381 caaagtccct tgtttcgaat aaatggtgtt caatcagctg accttcagca ttaactcaaa
    13441 aaacggcaaa atatgaggag ctattttttc gtttgttcct ctaatactca ggtcaaatcg
    13501 ctgattttac aacctcttcc ttaaacggtg ttctcctgcc aacacctcgg tcacaaggca
    13561 caatagtcaa caaacatgag aagttgtttt ttgaaattgc tccttgtgac tcggagttaa
    13621 acggcaggct cactacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    13681 gcccagcaaa acgaaaaata tgaggggcta tttttgtttg gctgttctta ttacttgatg
    13741 caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt ctgcgaaaaa
    13801 caagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    13861 aagctgacca cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
    13921 ctgtttctgg ttcagtgatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
    13981 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
    14041 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
    14101 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
    14161 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
    14221 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
    14281 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
    14341 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
    14401 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
    14461 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
    14521 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggaa
    14581 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
    14641 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
    14701 tgacataagg ggatattttt tccgctcctc caactgcaaa tactagcatc gaaacggttg
    14761 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
    14821 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
    14881 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
    14941 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
    15001 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccgcca gcaatcagga
    15061 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
    15121 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
    15181 tatccttgaa ggctgaccct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
    15241 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
    15301 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
    15361 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
    15421 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
    15481 acacatcgtt ttttcatatt ttttccatca aaa
//
LOCUS       DAZUMO010000065        15414 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000065 DAZUMO010000000
VERSION     DAZUMO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.065"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..2215
                     /locus_tag="ACG8ER_002114"
     CDS             155..2215
                     /locus_tag="ACG8ER_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS5050681.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2298..2867
                     /locus_tag="ACG8ER_002115"
     CDS             2298..2867
                     /locus_tag="ACG8ER_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5050682.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3042..3848)
                     /locus_tag="ACG8ER_002116"
     CDS             complement(3042..3848)
                     /locus_tag="ACG8ER_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050683.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3986..5500
                     /locus_tag="ACG8ER_002117"
     CDS             3986..5500
                     /locus_tag="ACG8ER_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS5050684.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5903..6886)
                     /locus_tag="ACG8ER_002118"
     CDS             complement(5903..6886)
                     /locus_tag="ACG8ER_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS5050685.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6891..7538)
                     /locus_tag="ACG8ER_002119"
     CDS             complement(6891..7538)
                     /locus_tag="ACG8ER_002119"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5050686.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7623..8255)
                     /locus_tag="ACG8ER_002120"
     CDS             complement(7623..8255)
                     /locus_tag="ACG8ER_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5050687.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8271..8822)
                     /gene="coaC"
                     /locus_tag="ACG8ER_002121"
     CDS             complement(8271..8822)
                     /gene="coaC"
                     /locus_tag="ACG8ER_002121"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS5050688.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8819..9556)
                     /locus_tag="ACG8ER_002122"
     CDS             complement(8819..9556)
                     /locus_tag="ACG8ER_002122"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS5050689.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9553..10542)
                     /locus_tag="ACG8ER_002123"
     CDS             complement(9553..10542)
                     /locus_tag="ACG8ER_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5050690.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10800..11342
                     /locus_tag="ACG8ER_002124"
     CDS             10800..11342
                     /locus_tag="ACG8ER_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS5050691.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11359..12444
                     /locus_tag="ACG8ER_002125"
     CDS             11359..12444
                     /locus_tag="ACG8ER_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050692.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12456..13262
                     /locus_tag="ACG8ER_002126"
     CDS             12456..13262
                     /locus_tag="ACG8ER_002126"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050693.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13263..14090
                     /locus_tag="ACG8ER_002127"
     CDS             13263..14090
                     /locus_tag="ACG8ER_002127"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050694.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14087..15160
                     /locus_tag="ACG8ER_002128"
     CDS             14087..15160
                     /locus_tag="ACG8ER_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5050695.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4956 a         2783 c         3139 g         4536 t
ORIGIN      
        1 ataagttatt attccgattt tcttgaaaca ataaaaaatc aaaaaaaata ctctcctaat
       61 agtccatctt cttttgattt tatttatata atgaaatcat cttatgaaaa ttactacttc
      121 atgtttagac aagagtaggg aggactaaga ataaatgtgt tcaaaaagac gttcaaaaca
      181 actaaataaa cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa aacactacgt
      241 taagtttatg atgcgcacga tgctgatcag tttatctgta ggtgtctata tgcatgtacc
      301 cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg agaccactga
      361 ttcccctatc actattactc atactttcac tgatgcaaat ggaaaacagc tacttcctgc
      421 attgacttcc tataataaag tcagttatcc tccaacgata aaccactact ggttaattcc
      481 taacaaaaca ttatggacat cggaaaccgg aagcagtgtg ccgctttcta tcttttccga
      541 tatgactgat cctacaaaag gtgatcagtt aaaacagatc actgatattt tgaatcatgc
      601 tttgacagat ataaaggacg gtggaagcgc acaagtttct tatgtatatg aaaaagatta
      661 ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt cgatatctga
      721 tctggtagaa aggctgaaga acgcagatgg aacacaagga gatatgtcca aagtaatttc
      781 aaacaatggg aatatcgtgg atacgactaa tacaggaaca tctactattt ccttgtccta
      841 ttttgatcca attgctttaa aaacaatgac tgcatctgct gtgatccatg ttcgtgctga
      901 tcaaacagct atcaaaggaa aagatcttca cttaagaaca ggagagagtt acgcccctaa
      961 agatcttgtt gaatatgtaa caaaaagcga tggaacttca ggaaataaag aagaaatcaa
     1021 aatgaacggg acagtagata ctacaactcc gggagaatat cccgttgtat taacttatat
     1081 agactcgaaa acatcaaatg tggcagaaac aacagctgta gtttatgtag aagcatctgc
     1141 tgcttcatcg actgtcatca ctcattttat agatagtcaa acaaataaag aacttcaagc
     1201 agaaacacat atgagtagtg taaatcgtat tcctgatttg cgcctacctg ctgaattttc
     1261 tgatcgagtg ccaaatatag atttatcgac ttatgaacaa aatgggaaag tcacttctat
     1321 ttctgattgg atgcaagcaa acggattaga cagaaaaagt cactggtcgg agatccttga
     1381 agtacttcac tcagaactga tgaataatag gaagcaatca ggaggggaaa ttacctttac
     1441 ttatgtttat ataccagaca atcgttcatt agaaggtcat gatcttattg ttcatccaaa
     1501 cgaaaaaata aacaaagaag agttgatcca atcagcaaca aattccttag gtaaacccgt
     1561 accgttaaag gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg gttatttgac
     1621 caaacaaaca ggtgatttta cgattatgta tagttatcat gacccgtatt cactccaaga
     1681 actccatgct cagtcactat tacatgtttt accattaaac aaacagatag atcaaaaaga
     1741 acaaaaaaga caagaagaaa gcttgtcaga aaaaaaacaa caaatggctc atgcagtttc
     1801 tccaacaggg aaagtaaaac aaacaagaag cctagagtta gcaagaatat ctagaaaaat
     1861 ctcagaatct aataaaacga taaggcaaga agctcatttg gccccacaag tttctggatt
     1921 ggaaaatgaa attttagagc ctaaaataaa acaacaatca gaaaaagaat ttgttgctaa
     1981 acagtcggaa gatcagaagg aatcagatga ccagaaggaa acgaagaaaa aacgcttgtc
     2041 aactattgat tccaccgatg agcatctgca gccgcaagta ccacttccta gtccgggaac
     2101 acccgcacct ggtggaggta ttccttcttc tgctttagga gatttcatgc gaggtatggc
     2161 aggaacatgg gtatacgcta atcgaaatga atttgacgca gatgactggt tctgatttat
     2221 atctctatct gttatagtca aataagtagc tgagtcatat gttatttggc ataatggaga
     2281 cgaggaggga tagaaaaatg ttagataaaa cgttacctta cgcagaaatc tggatgacaa
     2341 gaccacttca acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa tttcgatttt
     2401 atcaaaaaga agatgaatct gcttgggcgg agattgagac ttccgttggg gaatttcaaa
     2461 cgacagatga agcacttgag tattttcaac aaaactttgc tccttatcct gaagaacttg
     2521 aaagaaggat gttttttgta gagacaaaaa aaggagaaaa agtagcaacc tgtaccgctt
     2581 ggcgaaaaaa atcaagagag caagtgtatc cggtttttca ttggttagca gtgaaaccag
     2641 attaccaagg aaaagggctc gcaaaagcat tgactgccag agtcttgcaa gaatttccta
     2701 ccctccatac ggacggacca atctatcttc acacacagac atggagccat caggcgattg
     2761 ctctttataa aaaaatgggt tttactttta ttgctgaaaa tctagacggc acacctaatc
     2821 cagattatga aaaagttatg caggttttaa gtaaattaga taaataacga aatggtgaaa
     2881 taagtcctca gtattgggat aaaaaacgaa atagtcattc ctcatatctt acttttttga
     2941 gttaatacta tcggataact aatggcatgc catacattca aaaaagaagg ggcagtgaca
     3001 aaagtattgt cactgcccct tctcttgttt tttagcatgt tttatcgttt gattttttta
     3061 ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata gaggtaacca
     3121 aatacactga aaacaactgc tccaataaaa ttaacaaata aatccttcat agtatccatg
     3181 attcccacgt ctaagtaacc attttggatg attgttttat ctgttttacc atctgcattt
     3241 tgactttcaa tgattgtccg agtgatatct ggaacatgat agacagtgtt tccgtcttta
     3301 cttaactcca cactattgat ttgctggatg atcgtatctt tttgcatatc gaattgcagt
     3361 aatttatctg ctgaaaattc cacaaactcc cacataacac caactgtcat cgaaaaacag
     3421 aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg tttttgtatt tttattgagc
     3481 aaataaacaa gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc atgtagaaaa
     3541 gtatcccaca ttgaaaaata gccataaaaa tcacttaact ctcctaaaac tgttgaacta
     3601 aaaataaaag ataacaaaat gatttctagc atatttggaa tctgtatctt taatatcttt
     3661 tccagaaaat aagggatttc aaataaaatt aatgtcagta ataaaagcat tgtatttccc
     3721 cagtgttggt tccagacctg atccactagc ataataacga cgatgatacg taataaaata
     3781 tagattccgt ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa ggttttcaaa
     3841 tgattcataa aataactcct tcatgtatat ccttaattat actaagaagc gtgtgtttac
     3901 caaagctata tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc acttaatata
     3961 gtataaggag aaaaggggga tacaaatgaa aaaattagtt gctggtttat taacagccgt
     4021 tgcacttctt gctatgagcg catgcggcgc agatcagaaa aatacagcgc agacaagttc
     4081 tagtacgaag gaatctgccg aagtatcatc aggcgcttct gaacaagtat atacagatcc
     4141 aaaagaaatg aaagaatctt atgatgtcgt gattattgga gctggtggtg cagggatggc
     4201 agcagctatc gaagcaaaag atgctgggtt gaatccagta attttggaaa agatgcctgt
     4261 tgccggagga aacacatcta aatcatcaag cggtatgaat gcttcagaaa caaaatttca
     4321 aaaagaagaa ggaatcaaag actcgaatga tgccttctat gaagaaacgc taaaaggtgg
     4381 tggaggaacg aatgataaag aaatgcttcg atactttgtc gatcactctg ctgatgcggt
     4441 cgattggcta gatcaaaacg gtatcaaact ggacaactta acgatcactg gagggatgag
     4501 tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt ggtggatact tagtggaggg
     4561 gttattgaat aacgtccatg agcgggaaat ccctattttt gtcaacgcag atgtgacaga
     4621 cattaagaaa aatggggaaa cggtcaatga agtgactgta catgttcaag gagaagaacc
     4681 aaaagaggtc accggaaaag caatcgtagt taccacaggc ggttttggtg ccagcaaaga
     4741 atttattgaa aaatatcgtc ccgatttagc tgattatatt acgacaaatc aagaaggatc
     4801 aactggagat ggtatcacca tgattgaaaa attaggtgga caaaccgttg atatggataa
     4861 aatacaaatt catccaactg tgcagcagga tgagggggtc ttgattggtg aggttgttcg
     4921 cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg aaatggatac
     4981 aagggataaa gtatctgcag caattacagc attgcctgaa aaatcagctt atttgatttt
     5041 tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa aaggatatgt
     5101 cacaactgga aagacaatgg aagaattagc agaaaaaatc gatttaccaa aagaaacact
     5161 agcaaaaaca gttgatggat ggaatacgga tgttgcggag aaaaaagatg ctcaattcaa
     5221 ccgtgccaca gccatggaac atgacttatc gtctccgaat tattatgcga tcaaaatcgc
     5281 accaggaatc cattacacga tgggcggtgt gaaaatcaat acgaacactg aagtattaga
     5341 taaagaaaac cagccaatta gggggttata tgctgctggt gaagtcacag gtggcttaca
     5401 tggaaacaac cggatcggtg gaaattcagt tgcggagatc attatttttg ggcgacaagc
     5461 gggcatacaa gcagctcgat ttgcttcgga aaatgaatga aatagaacgt aaataaaaag
     5521 aggttgtgaa atcagcgttt tgagctaata ccgagggtca gctgattgaa cactgtttag
     5581 tgaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag
     5641 cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
     5701 ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     5761 cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt cacaaaaatt
     5821 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
     5881 ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt
     5941 tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa
     6001 tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg
     6061 cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac
     6121 gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa
     6181 tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc
     6241 cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt
     6301 gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga
     6361 gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt
     6421 ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt
     6481 gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag
     6541 cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag
     6601 catattcggc ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag
     6661 tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt
     6721 agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg
     6781 tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt
     6841 ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc
     6901 tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc
     6961 gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga
     7021 gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca
     7081 tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat
     7141 tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt
     7201 tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg
     7261 atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg
     7321 gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa
     7381 gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca
     7441 tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg
     7501 ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca
     7561 aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac
     7621 tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca
     7681 gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata
     7741 gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa
     7801 aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag
     7861 aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa
     7921 taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt
     7981 ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg
     8041 ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt
     8101 cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt
     8161 ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac
     8221 atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt
     8281 ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct
     8341 tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca
     8401 gatctcaatg tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg
     8461 gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga
     8521 tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga
     8581 aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc
     8641 atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa
     8701 tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt
     8761 gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc
     8821 attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt
     8881 gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga
     8941 tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg
     9001 cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg
     9061 cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca
     9121 caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg
     9181 caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt
     9241 cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat
     9301 cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga
     9361 tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt
     9421 aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc
     9481 ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg
     9541 tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc
     9601 agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag
     9661 ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg
     9721 tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt
     9781 cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat
     9841 caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg
     9901 ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa
     9961 agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga
    10021 ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc
    10081 agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa
    10141 catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg
    10201 tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt
    10261 gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt
    10321 actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac
    10381 ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt
    10441 taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa
    10501 aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt
    10561 aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt
    10621 agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa
    10681 gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact
    10741 aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta
    10801 tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac
    10861 taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt
    10921 caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat
    10981 tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt
    11041 atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag
    11101 aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt
    11161 cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga
    11221 cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat
    11281 taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt
    11341 aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc
    11401 tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat
    11461 acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc
    11521 accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct
    11581 aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt
    11641 tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag
    11701 aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt
    11761 gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg
    11821 aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg
    11881 cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat
    11941 gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc
    12001 gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat
    12061 tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt
    12121 gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt
    12181 gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg
    12241 gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa
    12301 aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc
    12361 tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca
    12421 cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt
    12481 ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt
    12541 atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta
    12601 cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt
    12661 ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc
    12721 aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg
    12781 catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga
    12841 tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg
    12901 agattccatt tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga
    12961 tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc
    13021 cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt
    13081 tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg
    13141 aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa
    13201 tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat
    13261 aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg
    13321 caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta
    13381 caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg
    13441 tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat
    13501 ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca
    13561 tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct
    13621 ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca
    13681 gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg
    13741 tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc
    13801 cttatctttc tccaggaatc atagctggat attttatggc atttacctat tctcttgatg
    13861 attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct
    13921 attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct
    13981 tgttctccat gatattggtc attggatact actttattag taaagaaaac cgtccaaaac
    14041 ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca
    14101 atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact
    14161 tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta
    14221 tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga
    14281 aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga
    14341 catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc
    14401 attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga
    14461 ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata
    14521 taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga
    14581 attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa
    14641 tagtttaggc tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa
    14701 attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat
    14761 gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg
    14821 ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa
    14881 tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat
    14941 gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga
    15001 agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga
    15061 tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta
    15121 taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat
    15181 ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa
    15241 tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac
    15301 gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag
    15361 taaatctaag attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaa
//
LOCUS       DAZUMO010000066        14919 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000066 DAZUMO010000000
VERSION     DAZUMO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.066"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..997
                     /locus_tag="ACG8ER_002129"
     CDS             236..997
                     /locus_tag="ACG8ER_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS5050696.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1225..1419
                     /locus_tag="ACG8ER_002130"
     CDS             1225..1419
                     /locus_tag="ACG8ER_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050697.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            1688..1987
                     /locus_tag="ACG8ER_002131"
     CDS             1688..1987
                     /locus_tag="ACG8ER_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050698.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(2084..2518)
                     /locus_tag="ACG8ER_002132"
     CDS             complement(2084..2518)
                     /locus_tag="ACG8ER_002132"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5050699.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(2750..3460)
                     /locus_tag="ACG8ER_002133"
     CDS             complement(2750..3460)
                     /locus_tag="ACG8ER_002133"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5050700.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(3450..4310)
                     /locus_tag="ACG8ER_002134"
     CDS             complement(3450..4310)
                     /locus_tag="ACG8ER_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS5050701.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(4315..4953)
                     /locus_tag="ACG8ER_002135"
     CDS             complement(4315..4953)
                     /locus_tag="ACG8ER_002135"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS5050702.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(4968..5267)
                     /locus_tag="ACG8ER_002136"
     CDS             complement(4968..5267)
                     /locus_tag="ACG8ER_002136"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5050703.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(5310..6794)
                     /locus_tag="ACG8ER_002137"
     CDS             complement(5310..6794)
                     /locus_tag="ACG8ER_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5050704.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(6814..7275)
                     /locus_tag="ACG8ER_002138"
     CDS             complement(6814..7275)
                     /locus_tag="ACG8ER_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5050705.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(7293..8360)
                     /gene="ulaG"
                     /locus_tag="ACG8ER_002139"
     CDS             complement(7293..8360)
                     /gene="ulaG"
                     /locus_tag="ACG8ER_002139"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS5050706.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            8578..9348
                     /locus_tag="ACG8ER_002140"
     CDS             8578..9348
                     /locus_tag="ACG8ER_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5050707.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(9621..10448)
                     /locus_tag="ACG8ER_002141"
     CDS             complement(9621..10448)
                     /locus_tag="ACG8ER_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050708.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            11015..12250
                     /locus_tag="ACG8ER_002142"
     CDS             11015..12250
                     /locus_tag="ACG8ER_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5050709.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(13249..13509)
                     /locus_tag="ACG8ER_002143"
     CDS             complement(13249..13509)
                     /locus_tag="ACG8ER_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5050710.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(13536..14837)
                     /locus_tag="ACG8ER_002144"
     CDS             complement(13536..14837)
                     /locus_tag="ACG8ER_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5050711.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4763 a         2999 c         2382 g         4775 t
ORIGIN      
        1 tagtttcaag aaaatttaca ggaaatttga taactgatat tatttacttt ttgaccacca
       61 tatacatgac ttttattttt gtttcttttt tagaatcaaa tgtgattaaa tagaaaattt
      121 tacaataata tgcacatata aataataaga tgatataatc aacaaagaaa atgtgatttt
      181 tttcactata ttttatcaca tgaaaattca ttaagaaagt aggaatcgaa gtattatgca
      241 cacaccagaa gaaattatgg aactcagtat tcatcttgga acaaaaaaaa tacataaacc
      301 tatcttagct aaactgattt taggttttat cggaggagcc atgatttctc taggttattt
      361 ggcatacgta cgagtctctg cttctatccc agaatctatg gctagcttga ctagtttagt
      421 tggtgcctcg gtttttccta tcggcctgct ggtcatcctt cttggcggtg gtgaattgat
      481 caccggaaat atgatggctg tggcgattgc ctttatggat aaaaaagtca cattctcaca
      541 gctggttaag aattggtcag tgattacttt tgcaaatatt attggtgcaa ttttcgttgc
      601 ctatttcttt ggacactttg ttggtttaac tcattcaggt gtctatctcg accaagtgat
      661 tcatattgct gatcataaaa tcgctgtttc ttggtggcaa gcattaggct ccggaatagg
      721 ttgtaattgg tttgtcggtt tagcgttatg gttatcatat ggagcaaaag atgcggccgg
      781 caaagtacta acgatttggt ttccagtcat gatttttgtg gcaatcggtt tccagcatag
      841 tgtggccaac tgttttgtga tacctgctgc catatttgaa ggtcaagcga cttggttaga
      901 atttctctcg aattttatcc ccgtttatat tggaaatatc atcggcggag ctatttttgt
      961 ttcaggtttt tattacgtta gttataagca tcattaagat tgaaggtaat gttcattcga
     1021 gcattacctt caatctcttt atatactatt tatattttaa gaacaatagc cttttattat
     1081 atatattatt caaattagtt atatcgagtg aaaaaaactt tcaaattgat tctttttatt
     1141 tatcataaag atatagatac ataatcattt tcacttacat atatttgaat attaactaaa
     1201 aacagcaaga aagaagagga tcaaatgtat tatgtagaag taaaaacaaa aggcgtcaaa
     1261 aacaaacaat atgtgaaagg tatcagtaac gaatatcctt tacttggttc atggaaagaa
     1321 gctgctcctt tttccaaacc atgtgccata aaaataaaga acgagctaga aaaagaactg
     1381 acttgtggca aagctgttgt agatatcatc gaaaagtaaa tccaatgtat agaaaggaag
     1441 atcacacatg aaaagcgacg tacaaattgc taagattata gaattagaag aagaagacgc
     1501 agtgttgaca attgaaaaaa aataaatgtt tgtataccat atgattattt caatatgtat
     1561 cctggaattg gagatctcgt agagctatat caagatgaat caaatatact tgtcattcat
     1621 catgaaatat agagacttat aaaattgttt atttttataa tgtatcagta tagaaaggaa
     1681 ataaaacatg gattatactg ttgaatttga cagtgaacta cttaagcaat ttccacaaga
     1741 agaacatttc atgtataaag aagcaatttc ccacaaagtg acagagtatt tggagcataa
     1801 tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa ggaatatacg agatgaaagt
     1861 caaaattaat aaaaaaaatt atcgcatagc atttgcttta aaaaaaaatc atgtgttcat
     1921 tttttatatt agttcgaatt tgcaaaaaat tgtatttgat aaagaggtga gtaaaaaaat
     1981 aaaatagctg agcattggag gatagattga gtgatactaa gtctatcctc ttgtttttct
     2041 aaagaactaa gctagaattt tttcttttgt acgttattat atgttatttg tcattaccaa
     2101 aaagcacctt ttttcttcta gtatctatga cataagaaat accacccatt tcctttactt
     2161 gaacaaatcc tactgattca taaaattttt ttgttttctc agtatcatct gtcaataatt
     2221 cgatttggac tggtccataa ctagtgatct tctccaaagc ttcattcaaa agcactttgc
     2281 ctattccttg gcgctgatac ctcggatgaa tgaggatatc ttgaataaat gcgatcgtcg
     2341 caaaatcagt gatccaacga acgattccga taatcttccc ttccccatta cgctttatca
     2401 gagattcaga ttgttcaaat gctctagcta accttgcagt gtcttttgtg taattcgtcc
     2461 aaccaacgct cttatataat tcagaaatct catcgatcat aagggaatca atattcattt
     2521 ttttcttcct ttcttataca attctccctc taataataca gttctttgac tatttaatct
     2581 atcacatata actctcctgt aaagattctc tcgattcctt aaaatctctc tttcctttta
     2641 gaatgcatcc tttttgataa ataaaaaggt tgtgataaat tttcttcaca acctctgttt
     2701 ctctctatca agtgttatct tttaattttc tacccttacc ctagcatctc tattgcccgt
     2761 agtatgcata ctttccatgt ttgcgaaaat aatgtttatc tagtaaatat tgcgggattt
     2821 cacatatatt ggggttgatc agctcgtttt ctttggccat cagacaaatt tcatctaaga
     2881 tcaaactgtt ttctactgct ttcataggtg aatcccccca tgtgaacggt ccatgtccat
     2941 agacaagtac tccagggact tcgtttggat ctaattttct ttccttaaac gtctcgacaa
     3001 tcacattccc tgtatgaact tcgtaagctt ctcttacttc ttctttcgtt aattggcgtg
     3061 tacaaggaac cttaccataa aaagcatcag catgagtcgt gccataagct ggcaaatcgc
     3121 gtcctgcttg cgcccacatg acggagtgcg tggaatgagt atgagtaata gcagttatat
     3181 cttcaaaagt ttgatacaaa acaacatgtg tggggagatc tgaagatggt tttaagctat
     3241 cttcttctat tatatttccc gacaaatctg ttaccaccat ttgatctgct tgcatgcatt
     3301 catatctgac accgcttggc ttaatgacga tgactcctag ctcacggtcc acttcactca
     3361 cattgcccca agtcaatttg actaatccgt atcgcggcaa tgccagattt gcttcaagga
     3421 cacgttgctt cattgctgca attttttctt cacaagacat catagccagc ctccttcaat
     3481 ttagaactta agtagtcttt tgcctgctgg atttctcttc gaaagtcttg gcttttttca
     3541 ctccacattt caatcagaaa tgttccatca taatctagac gtttcaaatg tcttaataat
     3601 ccaagaaaat caacacaacc gctaccaaaa ggaacatcac gaaactggcc tgaatattct
     3661 tttgttacag ggtaagtatc ttttaagtgg atagcagtga tataatcgat ccctttctca
     3721 agttctcgag ccggattatt ttccggccat gcggacaaat tgcctaggtc tggatatact
     3781 tgtagaaaag gggaatgaat ctgttctttg atttctaaaa attttgaaat ggagttcata
     3841 aatggatcat ccatgatttc cacggaaaga atgatgccat atttagaagc ttcctttacg
     3901 ctttctttta ttccttcgat aaagtcttct ctggaattca ttgatttctc ttcataataa
     3961 acatcgtatc cagctatttg gattactttg acgcttagat tatgcgccaa atggattgct
     4021 tttttcatta gttgtttcgc tgcttttttc ttttctggat cagcagaacc aaacggaaat
     4081 cgccgatgcg cacttaaaca aatcgaatgg atacgtacac cggagctgaa catctcttct
     4141 ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg ctaaccgctc atccgtctca
     4201 tcaatcgaca tctcgataaa atcgaagtcc atttctttta caagggaaaa acgttctttc
     4261 catgaaatat ctttgggtaa tgctttttca taaatcccaa tttgagccat ttccttaccc
     4321 ccagatacga atgatctcat ctttgaagtt ttgtgcggcc ttcatcggat catttgctgc
     4381 tgtaatacca cgaccagtga taaaggtata cacatcgatc ccttcaaata agtgtaatgt
     4441 gtgtggattt aatccaccag ttacagaaac attaaagcct agttcaatca gttttttgat
     4501 cttcgataaa tctttttcac cccaagtttc accagataac agcgcatcac ggctttgatg
     4561 gtagattacc tgccggatac cgatgttatg ccaatccatg gcttgttcaa aagtccaatt
     4621 gccgtagagt tctacttgca attcacctat ttctttttgt gctgcctcca tagttggaat
     4681 cgttgcacaa caaatgactg tcatccagtc tgctcctgct tttgcaacat tactcgccac
     4741 tgttcctcca gcgtctgcac atttcgtatc agcgactagt tttttatctg ggaacatact
     4801 gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa agaattgtgc ccacttcaat
     4861 aatatctact acatcaccaa ctttcataac atctcctaat gcatgttcta agtcattgtg
     4921 atctaacgca atttgcaaat tcggcctgct catcttagtt ccttccttta cttgtttttt
     4981 tggtaccact ttgtttcagc aaatttttca ctgatttctt tggctgacat cacatttttg
     5041 ataccgataa ctgtaacttc ttttttctct gcctgagtaa acatattcaa aaaattgact
     5101 ggcgtaaaaa ctacatcgta ttggccagct gaactttttc cctctgaaat ggcacaatga
     5161 tggattttgg taattggtac gtttagttct ttcattgctt tttccacact tctcatcatc
     5221 atcaagcttg ttcctgatcc atttgcacac gatactaaaa ttctcatctt aatccctcca
     5281 ttatgagtgt tgtctaatca gattttcttt tatactctct gcttttctgc atatgtgtca
     5341 tagtcttcta caatcaaaaa ataaccctca ggatttttcc gatattgtaa ctgcggaata
     5401 gccaataaac caatgacaac tacggcaatt ccagcataac ctaggacttt catgattact
     5461 gtaaacaatg gccaagctgt tgcccagtcg aacatcccga tataaccacc atatgttgac
     5521 atcccaatcc atgtagcgaa taatgctgaa ccgcctactt gtatgattcc tgaaagaaac
     5581 gggaacaggc aagcggcttt gaatccacct cggttattgg cataaactgc tataacggca
     5641 ttatcaaaga acaacggaat gaaaccggct atgacgatcg ttggtgattt caataaaatc
     5701 agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca agaacccaat cgttaccgca
     5761 tttggcgaac caaagccaaa agtagcagct acgtcaattc ctggaacagc tcctggtaaa
     5821 attgtgtttg agattccttg gaaagattga gttagttcat cgacgaatgt ccgaacacct
     5881 aattgcaaga tcgccagata taccgcaaaa tttaaagaag tcgtcataat gtagaataag
     5941 aaactctgtc cttcttgcat gaattgcgct tgaatcaaat aatctttgcc tagtacaatc
     6001 agaatgatcc caaagaaaaa tagcatgagt aacgaagtag caaccatatt ctcattgaaa
     6061 atcgacaaga atcctggtaa tttgatatct tcgatttttt tgtcttgctt gattgatcta
     6121 tttttctttt catcccgctt tttcatccac tcagctaatt ttgcaaatat aaagattccg
     6181 aacatttgct gatgtgccac tgcaaatccg gcaccttctg tcaattcctg cgtgattcct
     6241 actgttaagt tagaagctac tgcccaataa caacctaaaa tcagtcccat aaaaatcaat
     6301 acttgtatcc tacctagatc aggaaagcaa aacagtaaaa tccaaaaagc ggtagctgct
     6361 tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca gtttcgtata tttctgaaaa
     6421 cggactaata agatatttaa aacaaatgca atcagtaata aaatcatcac atctccaaag
     6481 gtacggccaa atgtctccat taatccagca tccacagcat tttgtccaaa ataaggatct
     6541 gtaaccatgg catccaaatt gaaccgttcc tttagtccaa ccaaaatcgg ccgaaaatta
     6601 ttgaccaaac caccagagcc aacagtcaaa ataaagtaac cgaccgttgc ctttaagaat
     6661 ccagcgatgc attcatgaag cgggcgtttc agcaaaatat agcctaataa cacgataaaa
     6721 ccaatcaggt atgctggctg cgtcaagata tttgttgcaa aatacgtcca aatgcttaac
     6781 aaaaattccc ccatcttttt tctcctctat caattaatta tattctgcca tcacaacttg
     6841 gtaatcatcg atggagttcg tagagatgag acgctcaacc aatccatctg tcattaataa
     6901 ttcggataat ttttggatat tttctagatg ttcttctgga tcttttgctg caagtgtaaa
     6961 aaacaaacta gccgttttct cctcatcctc cccttcactt tcaaaacgga ttggctgttt
     7021 caacttggta aatgaaatag ctgttccaaa aacaccatca cttttttctg aagaatgcgg
     7081 cattgctact tgaggtgcga tcacaatata aggaccgtat ttttctacac aatggatgat
     7141 ctcctccaca tacgtttcat caataatttc cttatcgatc aatacaagac agctttcggc
     7201 gatagcttca cgccaatccg tgatttcttt atctgggtaa tgaatcaatt gatggtcaaa
     7261 aaagtatttc agcaagaaac tccccttctt tcttataaaa acgacggaaa cggtgtatca
     7321 ttttcaatcg agaagacatc ttcaaatcct cgataaaaat taaactcaag atcatctttg
     7381 tttgtcggat agatgaattt cccgcctact tgccagatat aaggtttaaa tccatacttc
     7441 aggcgatcct ttttgaattt ccaaatttca gtgatctctt ttgaatcagc cataaaatta
     7501 gaccaaatat cgtagtggac aggaatcact acttttgcat tcaatgactc cgccatgcgc
     7561 agcatatcaa cagaagtaac tttgtctgtt atccctcgtg ggttttcacc ataggcccct
     7621 aaacagacat cgattttgtg ttcattacca tgtttggcaa ataaattgga gtaatgtgaa
     7681 tctcctgcat gataaagatt ccctcctgat gtttcgaata aatagtttac tgcaatttga
     7741 tccatctcct gaggcatttt tccttttaat acctcttctg gatcttcaca agtgaccaac
     7801 gctgtccgat caaacgcttc taaagctact acattgatgt ctttgatttt gactgcatca
     7861 cctggcttta ctgtaatcgt tttttcttca ggaattcccc atttttgcca cgtgcgaacc
     7921 acttcttttg gcccaatgaa tgttgcttca ggacaatttt gatgaactgc tgcggctgta
     7981 tggatatcta gatgatcgga atggatatgt gtaacgatta aagcgtctac attttttatc
     8041 gcaaaaggat cgattacgaa aggctgagta cgtaaattag gctgcatatt ttgaacgcca
     8101 ctcatacgca tcatctgatg gccttttttc atcatgccat tcccatgact acgttttccc
     8161 gtcccacacc atagatcaca taaaatattc gttccttcat gacttttcag ccaaattccc
     8221 gtacagccca gccaccacat agcaaaagtt ccaggatcca ctacttctgt ttcgatttct
     8281 tcattcaagt atgtgcccca ctcaggaaat gtttctacta accacttttc ttttgttact
     8341 tcattgacag ttttactcat ttgcttgctc cccccatatc gttgttccaa cttacaagta
     8401 aatattaatg tgtatcagca tccatggaaa cccttttata tgaccgtttg ttttttatat
     8461 gattttcttt cagtaatcaa tgattgtttc agtataataa agacagatat gaatgtttaa
     8521 atgaacaact agtcaaataa atccatggat caagatgtcc ctcaggaggc aaaagtcatg
     8581 aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa ttttggaact gcttcagcag
     8641 actgaacaac tatcaacttt cgaattagct gaatcccttg atgtatctat tagtacgata
     8701 cgaagagatt taaatttatt agaagaaaaa aacgagatca tacgcaatta cggttattgt
     8761 tcttataatt acaaaaacca gactgatttt gatcaatctg gtcctgaaag gattaaacaa
     8821 gcgattgcca gagaagctag taaatacatt aacgattatg atacgctgtt tatcaattct
     8881 agctcgactg cgttgaaaac actggatttt cttcaatcta accatttgac agtcattaca
     8941 aacaacgtga aaatcaatta ctctccgcac aaagataatt gtagttatat tttaactggt
     9001 ggagagatgc gctttccaaa agaagcacta gtgggcgaca tcgcattaaa tacgatttct
     9061 tcagtcaatg cagacatttg tatcattggc tgtagcggag tcgatgtagt acatggggtc
     9121 acaacaaaga tattaaatga atcaacgatc aacgaaatga tggtcaacaa gacaatcaaa
     9181 tgtaagattt tagtcgctga tcatcgaaaa atcggactga catcaaagtt caagattgct
     9241 gatatttcag tctttgatta tttgatcact gataaatttt gttctgaaaa agtattagag
     9301 gagatcagaa atacggggat aaaaaccatt caagtagatt cattttaaga accaggacgt
     9361 aaatatgtaa ataggattat caaaaaaatg aataacaaat ttatcgataa atgaaaaaca
     9421 cgctacgctc aaattgataa aggaatccca agatgtgtcc atccaatcga taccatcata
     9481 atctattgct tcttttataa ccgttatatg ttgcctaaat aggaatcaaa caatatcgaa
     9541 aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa taaacggtat tcaaatcaga
     9601 acactgattt cacaaccact ttatttcagg tcgttttcaa tcacctttac cgcgtaatca
     9661 tttccgttga aacgcgctat tttttttgcg tccatatatc tttctaaata atcatcttct
     9721 cctaattgct tatggcaaga agcaatttca taaagcaagt attccaatgc ataagtcgat
     9781 ttatgtttct tattatactc aatacccttc tcacataatt ggattgcttt atcataatcc
     9841 tcgattttac ggtaataggt ggcgatatca taatatacaa tgcagaccat cagattatca
     9901 aaatcagtct cactcaatat tttaagtgta cgctccacat aaactttcgc aaaatctaat
     9961 tctccttttg acaagtagat cattgccacg tttgcagctg cttgtaagtg atggatatct
    10021 gtttcggtcg tttgttccaa cacttgatta aaataaaata atgctgcatc tggttttttc
    10081 gcataaagat gatcaagcag tccttgataa aataaaattt gatttaaaag atcagaatta
    10141 cttacttttt cctgatcgat cgtttgtaaa attttataag attcttggta ttttgataca
    10201 gaattcaatg attgaatctc atttattttt ttcctgattt tgtcttcttc tgtttctact
    10261 aatatatcgg agatctcgac attcagcttt ttgcaaagcg cacttaagat attgatgcct
    10321 ggcgacatat tcttgttttc gatattgctg acagttgctt gttcacaaat gccttctgct
    10381 aattgtactt gtgttaaccc ctttttcttt cggtattctt ttagtaatgc tccatctact
    10441 ttaatcaaaa caatttcctt ccctttaaaa aattatttct cctttatatc atacacaaaa
    10501 aaaagttcta aacaaccaaa aatgtttaga acagaataaa aattttaatt ttaaaataat
    10561 attcaaatta aacaactggc gtatctgctc cttgatttct ttttgtgtta tctgaataat
    10621 tatcgtttac agttataaca catagtgaaa attgtcatgg cgagaaagtc taagaatatt
    10681 tttaaacata actatttctt tcttacgtgt gtataatgac gacagcacac ctatcaatta
    10741 atataattat tttatttttt atgttctcat gtttattttt aatgacacaa cttctcgtat
    10801 ttatctggca caacatataa atcgatcaaa aaattgttgc gtgttttttt gaaaattgga
    10861 gtttcaactt gattacaaaa tttaaattta gaagttgtga aaacagcgag agacagttta
    10921 atattcaaaa tatacatagg ctactcgaac gctgtgtttt ttagtatact agtaagaaac
    10981 gaagggatag actcattcca ataaggagga tttcatgaag acaattcttt ttgctcctgc
    11041 agtattcaat ttagctgaga caacacgaat gattgaggtt gccaaagcta tgaaagaccg
    11101 ttattactgt gtattctttg gcttttctaa aacttttgct cctttgatca cagaaagtgg
    11161 ctttgagtat catttactag cgccgatcct tacgaaaaaa caagaagaac aaatcataaa
    11221 atttgaccag ttgcgtacac ttaaaaaccc ttttaccaaa gaaatggttg cctctcgtat
    11281 cactagtgaa cagagtctga ttgctaagtt tgagcctcga ctgattgtga caggttcgaa
    11341 tgtgacaatt tttttatcgg cccgcaataa aaagattcct ttagtatatg ttaaacccta
    11401 tgctttgagt gaaccttttt ttcaatcaga gcatgcgctg atgccccacg agttacgttt
    11461 cttcggccct ttaagcaatc ccctttggca ctggacaaaa aagcgtatag taaatacccg
    11521 atggatcccc aatgccttca aacacgctgc acaaaaagaa atgcttcctt taccaaaaac
    11581 aagtttacaa atgatggatg ctgatctaaa tctcatcaca acgcctaaac tatttttgga
    11641 agaagaacag ctgccggaaa attatcgaat cgttggtcca atttttgcta agctcagtac
    11701 ttccttgcct gaagaagtgc taacttttat acagcgaaag caacaagaaa gaaaacgtat
    11761 tatctatttc gcaatgggta gttcaggcaa tccgcgtatg ttacgcaaaa ttttgttctt
    11821 cttacaaaaa caaccggatc tagcggttat tgcacctatt gactatctgc ttgatgacat
    11881 tcaatctgac tcagaagatc ttttaataac agaatatctg tctagtcatc ttttgcatga
    11941 attcattgat ttttctttta tccacggcgg ggaagggacc gtccaaactg cctgtgaatc
    12001 cggcaaaccg tttatcggtg tggggatgca atttgagcaa agctgtaata ttgactattg
    12061 tgtaaaatac ggaaacgcaa tcgccctcac aaaaccagtg actgaaagaa aactgcagat
    12121 tgctctgcgc gaagtttctt cacccaagat tcgtcagcag gcacgcacgc tgcaagccgt
    12181 ttttccaata aatggtccac aacaagcaat tcaagaaatc gaaaccttcc tgagcaacca
    12241 cccaatatag tttcaagtag aaaaagcttt tttaatagag actcatcagt aatttcgcta
    12301 ttcgagaccc agacagtgaa aatacaaaaa aagtaaatag tggattgaaa agttagagtt
    12361 acttaaatta cggtgtctaa aaaacacaaa atcatcctta aaataaaaga tctgacgcaa
    12421 tccaaatgag cataggtagc gtctgccatt tattcttagt caaattcttt gacacctttc
    12481 tctggttgga tcatctcaac gtttcttcaa aagaaagacc ataataatta ggcaaataca
    12541 ttttcttaaa atgtgtcata tagatctaga agcatcgctt tgtagagatt tgtgctgcta
    12601 gtcccttcaa caattactaa atcaaatgta ggatttacca ctttatcttt gataatcaac
    12661 ctccctcttc tgttgagaca atgatctcgc aatccgatgc atactcctat ataaactgcc
    12721 agcaatcccc acatgctgag tagatcatgt cagaatgttc tatgtcgtca gcaacgataa
    12781 actaaaagtc acgttcacca tgactgattg ctattcccag cgcaattatt tctatacacg
    12841 tttcatgatt tgaataaaca ctctcatcat acatattacc attcatacac catactgcag
    12901 cccctactat atggttgtaa gctcccaaat ctatatttgc cttgatcact ttggtagtag
    12961 atcgactaat tcttaatcca ttgctgataa aattttcatt tttgcctcct tctttctgcc
    13021 aaaacagtta aaacaataat aatctttatg taaaaaaggg ctgtgacaaa agccttgtca
    13081 cagtcccata ttccgaataa acggtggtca aaagcttgca tctgcggtaa taagttcaac
    13141 caaacgaaaa ataagccaac atttcacaaa aatttaagaa gcaattttcg taaatttctt
    13201 cttatttctt gaagctaacc acttctgtca caaccacttt tagaacattt aaagcttcga
    13261 tgtgacaaac catctatgct gctcgatcat aaaattgata gcaaaaattc cacaaagtac
    13321 atatgtcccc catgaaaaca aaacattcat atgcggtaca atgatctttg gaagaatcac
    13381 gatcaaaaat gatgccagtg cccaaatcca tcgattgatt tgaatatgat atgtagctag
    13441 catattcaat ataaggtgac tagccgttaa taaaaagata aaaacaaaaa ataaaatcag
    13501 ccaatacatt gtttcccacc ttctaaaaac caaacttaga cagtttggac ttcgttttct
    13561 ccacgacttt gttcatctga cagcatcttt ttatcataag cttttacaaa tggataatag
    13621 ataataaatg ctatcaatgt gttgatcatg atcaagataa caacacgcca gtcatttcct
    13681 gctgaaagat aaccgccgat tggagctggt aatgtccaag ttggcaaaac agtaaatcta
    13741 ttgaccattt ctaatttgac tgcaaaataa ctgataatag tcaaaactaa aggaactaag
    13801 ttgaacggga tgaacatgta aggattcaac atcataggag ctccaaaaat catgggctca
    13861 ttgatattga agataccagg aataatagaa aaacgaccaa cctctttcaa gaattttgat
    13921 ttacagaaga atataaacaa aacagatacc acgatcgttg ctcctgcacc accaattgtt
    13981 accgtccatt gataaaactg ttcaggagcg atatagggta attgggtggc agctgtacca
    14041 tctgccaatg cctttgcatt ctcatccaac atgatcagcc acattggacg aacaacagcc
    14101 cctacaatac tcatgccatg gataccaaat gaccacaaca aatggatcaa aaacattggt
    14161 agtaaaacac caagcaaatt attccctaaa atattggtta aaggctcgaa taacgataat
    14221 aaggtagcgt ttacatcaaa gcccgcgacg cttcgaatca gccaagtaat tgttaaaatg
    14281 actgcacctg gaatcaatgc ctcaaaacta cgggcaacag caggtggcac ttgttctggc
    14341 attttgatag taatatttcg atttttaaag aaacgatata cttccactgc aaaaatcata
    14401 gataagatcc ccgcaaacat cccagatgct cccaaagaac tcataggtaa gacccagcca
    14461 agaggtgcct tttcaggagt taaaacgttt acaggaactt gtaacataaa gaacgttcct
    14521 aaagataata taccacctgt cacactatct aatccatatg atttagctag atccgaaccc
    14581 ataacaaaag ttgcataaat agccatcaat cccatcgtca tcctaaaagg taaaacaatc
    14641 gttgccgaat aaggggcaat aaattcattc catccaggaa acggtaaatt gataaagacc
    14701 ataaaaaatg tcccaatcaa aatcaatgac aatgttgaaa caaccccatt acgaattgct
    14761 aataaatgac gctgtgtacc aatttttgcc attggtggga caagtttctc ttcgacccat
    14821 ttcatcagct tttccactaa aaccactcct tttcaataaa atatgaacca ttcattgcat
    14881 gacaacgcaa agaatgttca taactaatta tataattaa
//
LOCUS       DAZUMO010000067        14824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000067 DAZUMO010000000
VERSION     DAZUMO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.067"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..400)
                     /locus_tag="ACG8ER_002145"
     CDS             complement(101..400)
                     /locus_tag="ACG8ER_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050712.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(403..564)
                     /locus_tag="ACG8ER_002146"
     CDS             complement(403..564)
                     /locus_tag="ACG8ER_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5050713.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(579..938)
                     /locus_tag="ACG8ER_002147"
     CDS             complement(579..938)
                     /locus_tag="ACG8ER_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS5050714.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(935..1786)
                     /locus_tag="ACG8ER_002148"
     CDS             complement(935..1786)
                     /locus_tag="ACG8ER_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5050715.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1803..2636)
                     /locus_tag="ACG8ER_002149"
     CDS             complement(1803..2636)
                     /locus_tag="ACG8ER_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS5050716.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(2643..3329)
                     /locus_tag="ACG8ER_002150"
     CDS             complement(2643..3329)
                     /locus_tag="ACG8ER_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5050717.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(3335..4081)
                     /locus_tag="ACG8ER_002151"
     CDS             complement(3335..4081)
                     /locus_tag="ACG8ER_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5050718.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(4074..4415)
                     /locus_tag="ACG8ER_002152"
     CDS             complement(4074..4415)
                     /locus_tag="ACG8ER_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050719.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(4473..4601)
                     /locus_tag="ACG8ER_002153"
     CDS             complement(4473..4601)
                     /locus_tag="ACG8ER_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050720.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            complement(4594..5301)
                     /locus_tag="ACG8ER_002154"
     CDS             complement(4594..5301)
                     /locus_tag="ACG8ER_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS5050721.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            5346..5825
                     /locus_tag="ACG8ER_002155"
     CDS             5346..5825
                     /locus_tag="ACG8ER_002155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050722.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(5884..6054)
                     /locus_tag="ACG8ER_002156"
     CDS             complement(5884..6054)
                     /locus_tag="ACG8ER_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050723.1"
                     /translation="MGKFNRTLVFSAPLIIYALGLWGSRQALIGTIVYMLWIFIGLDE
                     AEYRAKKPVGRD"
     gene            complement(6066..6332)
                     /locus_tag="ACG8ER_002157"
     CDS             complement(6066..6332)
                     /locus_tag="ACG8ER_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050724.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(6343..6534)
                     /locus_tag="ACG8ER_002158"
     CDS             complement(6343..6534)
                     /locus_tag="ACG8ER_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050725.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            6825..7247
                     /locus_tag="ACG8ER_002159"
     CDS             6825..7247
                     /locus_tag="ACG8ER_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5050726.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7264..7686
                     /locus_tag="ACG8ER_002160"
     CDS             7264..7686
                     /locus_tag="ACG8ER_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5050727.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            7715..7915
                     /locus_tag="ACG8ER_002161"
     CDS             7715..7915
                     /locus_tag="ACG8ER_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050728.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            8002..8451
                     /locus_tag="ACG8ER_002162"
     CDS             8002..8451
                     /locus_tag="ACG8ER_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HFS5050729.1"
                     /translation="MKKVITSLLVFASLLLFTACGKKVTTEDLKANDWLIEATKDDEP
                     NMIVSFSDHVVSFSVDTSSMKSTSSNDWEAMGEEFAKSLVDQMNYKFEYTLEGDVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKPNKSDDNEKLVLKPYKKEKN"
     gene            8753..8890
                     /locus_tag="ACG8ER_002163"
     CDS             8753..8890
                     /locus_tag="ACG8ER_002163"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5050730.1"
                     /translation="MTLQPGEGTKQIAERAGITVDQLFALNGMDPNNYMLYPGQELRV
                     K"
     gene            9028..10233
                     /locus_tag="ACG8ER_002164"
     CDS             9028..10233
                     /locus_tag="ACG8ER_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS5050731.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(10285..10372)
                     /locus_tag="ACG8ER_002165"
     tRNA            complement(10285..10372)
                     /locus_tag="ACG8ER_002165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10338..10340),aa:Ser,seq:cga)
     gene            complement(10517..11083)
                     /locus_tag="ACG8ER_002166"
     CDS             complement(10517..11083)
                     /locus_tag="ACG8ER_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5050732.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            11338..12669
                     /locus_tag="ACG8ER_002167"
     CDS             11338..12669
                     /locus_tag="ACG8ER_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS5050733.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            12635..12985
                     /locus_tag="ACG8ER_002168"
     CDS             12635..12985
                     /locus_tag="ACG8ER_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050734.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(13077..13496)
                     /locus_tag="ACG8ER_002169"
     CDS             complement(13077..13496)
                     /locus_tag="ACG8ER_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS5050735.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(13497..13841)
                     /locus_tag="ACG8ER_002170"
     CDS             complement(13497..13841)
                     /locus_tag="ACG8ER_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS5050736.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            14191..14787
                     /locus_tag="ACG8ER_002171"
     CDS             14191..14787
                     /locus_tag="ACG8ER_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5050737.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         4387 a         2817 c         2421 g         5199 t
ORIGIN      
        1 aacttaacta attccgctcg ttcttttgct tttttatctt tctgaatctt catcatgatt
       61 ttcttgagga tgatatcact gtattgtgta atgagatcca ttatttctcc tccacatacc
      121 taaactgtcg cccctttgaa tcaatccata agctcctagc tctatcccag atgatgtttt
      181 tgctcagtcc agtaatttca gataactgtt cagctgtacc cgttactaga attcgatcac
      241 catgccagat tgcaattttt ctcggcgttt tccgtttagg cttttcagtc cacattgatt
      301 taccgagctt ttggacttct gcaactattt ctttgtcttc ttgccaattc tcagaatgtg
      361 tcagttcaat gattcgtttc attgctgctt tcttacccac gctcattcct ccaatctacg
      421 aatttccctt cttaaattca ctatgtgcaa attgattgcc tttctcgccg tttcattgac
      481 catcactgcc tttatccgtt ccagatcgtc aatctcacgt tgaaggcttc gaatacgcat
      541 ttgaatcact tcttctgttg tcatgatgga ccacctcgtt aaaaacgctc ttccttgaac
      601 gtattccgat attttttagc taatatcaac ggcacttgat attgatgaca gaacaacttt
      661 gccttgatct taaagtcttt tgtctgcatt cctttgacat ctacgacttt gacaagtttg
      721 ccgtttttat aaaatgtgaa gtcgggaata tactcgatct tgcgatactt ttttccatct
      781 agttcaaatt tcggcatcag ctcaaatctt tcctgaagtt ttactttcca gccgttcgct
      841 tctgcttgcc acaaggctaa atcgtaatac tctgcttccg cgatagaatc aaacttgata
      901 cctcgatgaa cagttttctt attacggtat ttattcatgc gatactacct ttcactggtt
      961 ttatgcgctt gtctgctgtt tgttggaatt tcagcgcata accttctgaa ttcttaaata
     1021 ttctagaaac aattctttcg ccgtaggctt ctcttagttc aggaccggat aagtttgttg
     1081 tgatgatcgt tgccttgttc tgtctggctt ctaagagcgt gtttaacgtg ttgtttgtaa
     1141 actgcctact atttgatacc ccgctaccta attcagctcc aatatcgtca aaaaccacca
     1201 aatcagttgt tttgatatcg gctataagcg atccttcaat ttcttttctc agttcagcat
     1261 tgttataaga aaactttatt tgctctaata actcttgata gcttataaaa agtattttct
     1321 tgtcataatt tgagcgctca agtatttccc aagctgtcgc cattgacaag tggctttttc
     1381 cgcttcctga tttccctgat agaatgaaat gtgcaggatg gttcagtaag acatcattta
     1441 caaagctttt agctctttct aaagcaattt tcgtttcttg gtccactacg tgataattct
     1501 ccattttgca tttaaacaaa gttttatctg ttaataccga accattttga aaaaaactca
     1561 acgctcgtgc ttttaagctg tcgttatata tccgttcggt ctgtatatcc tctttcacac
     1621 gtaacgcttt ataaccacaa ctcatgcatg ttggtttaca acgttctgaa ccatccttat
     1681 ttttagctcg ccaactatac aaaggttcgc tacattctgg acatttcccg ctttgcacta
     1741 atactcttct tattagtttc tccatagcat ttgctaggct ttccatgtga tgcatctcct
     1801 ttttaaattg gcaagtcgtc atattcacta ggattgctgt actgtagttt ttgactttgc
     1861 tttttatgat tattcttgtc tgctttgatt tcgaatttga gcttctcaaa tttttctctc
     1921 aatttcttag cacttctaat atttccaaac caaaattcat ttgtaggtag ccaattgatc
     1981 acatactcaa tcgcttctat agacgcttta tctctttctt ccatcaacct gattgtgtct
     2041 gcccattttt cgatatctac tttgttcatt tcttttggaa aatcttcagt taaattactt
     2101 tgcaattttt tagcaaggcg taagtgttcg tcagaatact tacctttctt ttcttcttta
     2161 tctatatcta tatctttctc tatctctatc tctaactctg gtgtagtttt gtctggacat
     2221 ttgtccgaca cttgtcctcc agttattaaa ttccgttttg cctcttctat tttctttctg
     2281 tattctcttt ttctatctgc ttcagttgag gattttccaa tgaaactttg tatatcagac
     2341 atataaattg ctccgttatc taatacgtca ataagctgca aatcacggaa aatttgtacc
     2401 gctttttcta cgactcctac agaatgtctt gtaatagttg cgagcattgt agagttaaat
     2461 ggaatcctgt cattaaacat caacttacct tcgtgtttta gacttcttaa ataaagtttg
     2521 agaagaatat tagaataaat atagccatct ggcatacttt ctaagagaac catctcgtca
     2581 ctatcgaaaa aattctcttt tagttttaaa tagtagtagc gtttgttgtc agacaatatt
     2641 ttttaccctc ctattctaag tttcttaatt gtttcctggt ttaacttgat ccctttgatt
     2701 tgatatttat ttttgaaatt gatcacacct atattgtgtt tctccgtgtg atgaatcctg
     2761 cagagtgctg caaatgtgta ctctgaatga tcaacttctt tgcgctttcg tcttcctagc
     2821 gctttgtcaa agtgatcgat gtcagctcct gttttgccac agatgcagca gactcttttt
     2881 gtgatgcatt tgtagaagta atattcttga ttcgctggta aaatctcata gccttctttg
     2941 aaaggaatat gatgttcaaa gatgaaatct aagatgatat ttgctaagac attagcatca
     3001 ctcacagttg tattcgattc gtctttgagg cttattttgc gccctgtgac gccttcaaaa
     3061 cggaagtaaa agaattcctt ccagaagtcc gttggcatgc ctgtatcgat gaaaatatcg
     3121 cctatcagcg catagatgaa gtttcgttgc tgcacagtaa atcgacgtgg atcaataaat
     3181 cgaacttcaa taatccgatc gccatcatat ccgtcataca tcgtctttag tcgatcaatg
     3241 ttcacttctt cattgatcgt tgcacctatg tcttttcctt tgaacttttt cagaaccgct
     3301 gaatatgaat cgatcaatgg tttaaacact catatcactt ctcttttgtt tcttctctgt
     3361 actgatcttc aatccagtta acgcctcgtt ttagaatgcc caagtctctc ttggtccatt
     3421 tactgtcatc agcggttata gaagccgcat cagtcaatgc aacaattgct tcatcaattg
     3481 atttttcgta cttgttagca accagttgta aagcatccaa gaatagcttt ttgcttcttt
     3541 gagtagctgg ttcaagcatc gagacatctt ctggcatatc ttcgccagca aatatatata
     3601 gccctagccc aaacattgct agatttttta caagacagcg catgatcgtt ttgttgatat
     3661 caaacatagt tgctgcttca actcgctttt cgatttttcc aacaatctct tttttcttcg
     3721 tttcgttatt ccactgataa tcattgactt cgtaggtata tggctcatct ttcattgcct
     3781 tgtttgcacc atccatgact ggtaaccaca tgtcacgctt tactccgttg actgtgatac
     3841 tggtaaaaac catatagcct gttttcttat caaagacata cggcagatat gtttctggat
     3901 catgatagat ttcataatct actgcctcac acactttgct aacttctgcc caagcccatg
     3961 cccacgaaag ataagtgagg ttatttcgtt tttcagtaac atctttcaca tttattttgt
     4021 ataaggtatt aaaaagcgta ttatcacttc tatttttcaa aggttgttcc ttttcactca
     4081 tcaaattctg cctccatttc agcaatgtat ttcttacctg atccgtaata agagatatca
     4141 atcaagttat ctctgtcata ctcttctagc gcatcaatca agccatcttc gatgacatag
     4201 atatattcag tttttttgga cttcctcgat aaatggataa gatagacatg atcccaaata
     4261 ctcacaaaat ttcccaaatc gtcttgatca catgctagtt cttcatccgt caaaagatta
     4321 cgtctgattt ttcgattgct tgtttccttg atattcgatt tgccccaact aggatcagtc
     4381 aaatattgat ctagagtgga aagttctttt ttcatgtggt aacatctcct tagatgtatt
     4441 ttctttgtga ctcattgctt tggtcggctg agtcactttt ttatttgttg ccatgctttt
     4501 tgcttatcaa tatgttgttg gcttaggatg tttggtttat tgtgtctcca ccagcgatta
     4561 gcaattatta cgcctatttt tagcgcttca gctctattca tcctctaaca actccatttg
     4621 cttaataatt gtcatcgtcg ctgtagaagg catccaattg ttaataaaat cgataacatc
     4681 atcgaactgt tttgccttga tacgatttct agctactgct ccagtgattc gcttaattcc
     4741 gccattcaag tctttaaaca attctgcttt tgctttgcta tttaaatgct gctgattaca
     4801 aataactcta attttgttgc gaaccatatt agaaattgtt cggtaatctg gttcagctag
     4861 aagttgattg ttcttcaaat cgtttaaatc agactcgata gcgtctactc tttcattcgt
     4921 ttcttcgttt gcagctagag caagtaatgc catttctcgt ttagttgctg gaattgatgg
     4981 caattggtct tttaattctt tttccatctt gttgaaggcc tcaatatatt gaagtttaaa
     5041 acttaaagct ttcttaccag tgaagcccat tgctaataga gagaagccgt ctctattcat
     5101 ataaataatt cggtatgatt gtttattttg aggatgaaca taagtatctt cccaaaatag
     5161 gtctgcccaa ttttcggcaa ccccctcttt tagttcatca atagccgtca aaacatgtct
     5221 gtgcttttta tcgaagcttt ctgcaacttg taagctcgta gtcacagctt ctttattttt
     5281 caaaattact aattcttgca ttatttcttc tctccttttt gatataattc tggtggggtg
     5341 aaaacatgag cagaaaaatt gataatataa tattgttaac aactattttt tatttagtaa
     5401 tcggagcaac tctttcctta ctgtcatttt tgtccaacag tagtttgatt gtgttaaatc
     5461 aaaaaagcat attaacagaa tttgcccaca tagattactt ctcttttttt aaaactggac
     5521 taatatgttt atttctatct tcatttatcc tctatatagg aactacattt tgtctaatgg
     5581 tgcttaattt aaaaatgttt ataattgaaa aatggatagt tattatcgca aagattttct
     5641 catcgatatc aattatcatt ccaatgacat tgacgttttc taaagagcaa tttaacgtag
     5701 ctactacatt cgtatctttt atagctctat tttcgtttgc tgttcctaaa acttttcaaa
     5761 gcaaatctag caacgcaaag gttaatgtag aaaacactga tagaaaaaat gatgaaagtt
     5821 attaaaaaaa ataattcttt catttattgt caaacctttc ttatagacat tagacttttt
     5881 tagtcagtcc ctcccgactg gctttttcgc tctgtactca gcttcatcaa gcccaatgaa
     5941 aatccaaagc atgtaaacga tcgtgccgat taatgcttgt ctactacccc atagacctaa
     6001 agcgtagata attagtggcg cgctgaatac taatgttcga ttaaactttc ccatgttgac
     6061 ctcctttaaa ttttgtattt agccatcagt tcatcgatgt cctttttgtc atatcttaaa
     6121 aagccatcta tacgaatttc ttttaaaccg tgagctatta acttctcaaa accttgatcg
     6181 tttacacctc ccacatattt tctagcttcg atctttttta aatatcgtgt ttctgacaaa
     6241 ttcttttttg aaagttgact aagtgcgtcc tttacaactt caacaattgc aattcttaaa
     6301 cgagtcaaaa actcttcgct tagaatattc atgacttgcc tcctatcgaa ttctgtaatc
     6361 acggatgatc tctagaatag ttctgtttgc ctttggtcca ctccagtgtc catcaagaat
     6421 ttgttgcatt cgtacacgcg tgtgaccata agcagtagcc aaatcatcca tagttattcc
     6481 attctctttc ataaactctt tgatcgccgc acgaccgtta tccaagtttg acattaaccc
     6541 acttcctttt tttataaatt tgtaagttaa aatgatagaa aaaatataat ttccattgac
     6601 atttttacac tatagagtaa aatgaataca tagagaaata agcctataac aaaacctttt
     6661 atttgcactt ggtcgccaaa ctttatgcta taaggtgtgt ttttagtttg cttttttcta
     6721 tcaaattaac ttacggataa ataataactc tatatcgtaa aattgtaaag ctatttttac
     6781 tctttttcgt aaattatttt ttcgtgttgg aaaggaacgt tggaatgaca acatttgaaa
     6841 gaataaaaga actagcgaaa aacaagaata aaaaccttca acaagtcgct aaagatttga
     6901 agtacggaga gaattacttt tatagtttaa acgcaggaaa acagccaact gccgaaaagt
     6961 tacaagaaat tgctgactac ttcgacgtat ctgtagatta tttgctagga cgtactgaga
     7021 aaaaaagata ctatgattta acagaaaaag acgagcgtga tatccaaaaa gaattgcagt
     7081 cattaatcga cgatttatct aatgctgatg gaatggcttt ttcgaagaaa gataatgaaa
     7141 tgagtgaagc gacacgtgaa gctctcatta tttctttaga gaatgctttg cgaatagcta
     7201 aaatagaagc taagaaaaaa tacacaccta aaaaatatcg cgattaaaag gagtacgttg
     7261 tttatgtatt tacctcaaat tgataacaaa attaataagc tggtaaaact atatcaaaca
     7321 cgcaatcctt ttagaattgc acgagaattg aagatactag ttttagaaga agatttggga
     7381 gaaatttatg gttactatag taaattcaaa cagataaaaa tgatccacat aaattctagt
     7441 ttagatgaga tgggaaaatg gataacttgt agtcatgaat taggccactg tgttttgcat
     7501 ccgaatgaaa acacacctat gttgtctaaa aaaacgctag tatctgaatt aaaaattgaa
     7561 aaagaagcaa attatttcgc aactcatctg actattgatc caactttaga cggctttgat
     7621 catatgacaa aatatgaaaa gctcgtctgc tttggattac cagatgaatt cgatcgtttc
     7681 ttgtgaatta taatacaacg taaggaggat atagatgaaa ataggaatga gaaaaccaag
     7741 tataaaaaaa tccataagcg ctagaaccac tggaaaagca aaaagaaaag taaaaaaagc
     7801 agttattcct ggttatggga aaaaaggtag cggctggatt aaagacccaa aaaaggctgc
     7861 ttacaataaa gtatacaaga aaactacatt cagtttttgg gatttattta aataaaaaaa
     7921 gcccgtgcag caacacggac tcgaaacctt atttctaaga ttccccaata aaaatcatat
     7981 catagaaatg aggaataaaa aatgaaaaaa gtaataacaa gtttattagt ttttgcttct
     8041 ctgttacttt tcactgcttg tggtaaaaaa gtaactacag aagatttaaa agctaatgat
     8101 tggttgattg aagcaaccaa agatgacgaa cctaatatga tcgtatcatt ttctgatcac
     8161 gtcgtatctt ttagtgttga cacaagttct atgaaatcaa cttcaagtaa cgattgggag
     8221 gctatgggag aagaatttgc taaaagctta gtcgaccaaa tgaattacaa atttgagtat
     8281 acacttgaag gcgatgttat gacttggaaa gatgataaag acaaagataa tgatgccaaa
     8341 tatactgtca gcaaagaaga taaaaatatt attttaactc cagaaaagcc taacaaatct
     8401 gatgataatg agaaattagt tttgaaaccc tacaaaaagg aaaaaaacta aagaatctag
     8461 aacaactact agctcaacaa gtagcaacga acaatcatct tcgactacaa cagaaagtac
     8521 tattgcatct tctagtactg tagaacaagc tgctattgcg cagaatacgc ctcctgaaac
     8581 tagtagtact tactctgctg aaagtgtaga aaactatgca gaagctactc ctagttcaag
     8641 tgttgaacaa gtttcaccca ctcccagttc aacggagcaa tcagtaccac aagattatgt
     8701 accagctaat caagctcaac caacggcacc ttcttcatct gaaaccgaaa ccttgacttt
     8761 gcaacctggt gaagggacca aacaaatagc tgaacgagct ggaattacgg tagatcaact
     8821 gtttgcatta aacggtatgg atccaaacaa ctacatgctt tatccaggac aagaactaag
     8881 agtgaaataa aaaaagcaca tttgccgccc gaccaagaac gcaaatgtgc aaagagctac
     8941 ggtaaagtag gtctatttgt tgtaccctat tttaccatct cgaaatccgc tgtacaaccg
     9001 aacaaacgta cgaaaggaaa gataaagatg gcaaaaaaag aaattgataa acgaattaaa
     9061 aagcacttaa caaaaaaagg cgaagaaaaa tatcagttta gtctgtactt aggagttgat
     9121 cctctaacag gaaaaaaaag aaaaacaacg agacgaggtt ttagtacgcc attagccgct
     9181 gagagagctt taaaaagatt ggaggcagat atacaggaaa aaggactgca agcttctaca
     9241 gctcaaaaga gcaagaaatt cgaatatgtt tacggcttat ggtttgaaaa ttataaaaag
     9301 acagttaaag aaagcacttg gtcctccacc aaacaaattt ttgacacaca catcttaaaa
     9361 gtgtttggag ataagttcat tgacaaaata gacgtttttt tctgtcaaga agcagtaaac
     9421 acttggtccg atagccatcc taaaattttt aagaaaataa aaaattacac atctaatgtt
     9481 tttgattacg ctgcttcttt acaaattata actagcaatc ctatgaaatt agtttctatt
     9541 ccacgtggag aagctctaga tatcgaagat aaaaatatcg aattttatac taaagaagaa
     9601 ttaatcaaat ttttagaagc aataagaaac gatgatgatg aaagatattt attcttttcc
     9661 ctattggctt ttacaggtat aagaaaagga gaagcttttg ctctgacctg gtcggatatt
     9721 gactttaaga gcaaaacact caatatcaat aaaactgtaa caagaggtta tcaaggaaga
     9781 ttgatcgtaa atactcctaa atcaaaatct ggaaaaagaa aaatttatct ggacaatgat
     9841 ttgatcagtt tattgagaaa gtattataca aaaaacaaaa cgatcgttac aatccaaagg
     9901 gaaaacttaa tttttcacca tgatggtctt ctctataacc ctacagtttc acgttcgtgg
     9961 cttaatgtca tatataagca tcatccagaa ttgactaaaa gaatcactac tcatggtttt
    10021 cgacatactc atgcctctct cctttttgaa tcaggagctt ctttaaaaga tgttcaggaa
    10081 cgtttagggc atgcagacat tcaaactact tcaaatattt acactcatgt aactgaaact
    10141 caaaacaaaa aagtcatcaa taattttgta gcatttatga agaacagtac tcagggggag
    10201 tcaaaagggg agtcaaaaaa cattttaggc taaatataca acaaaaaaga cacgatcgaa
    10261 atcgtgtcta atcgttgata tataaggaag gtacaggatt tgaacctgcg cgccggtatt
    10321 agccggttcg ccggatttcg agtccggtgc attaccactc tgccaacctt ccgtaagtac
    10381 tatttttaaa cttttttcac gaaatgtcaa gcctatttct aatagataaa taacagaaat
    10441 cgcttttttt agcaaaaaag tccattgaca gtttgcagcc aacggactta ccgatcaaat
    10501 tcatactgat agaacactat ctgattattt caggattatc taatgctaaa atcgagtcaa
    10561 ttgtccgttt gatcagtgca atgatttgtt cttctgttgg atgcatgatg tttgatgaca
    10621 tcaatgacgc taagccagtc actgctaccc atgtaccagt atgcagagca tcgacatatt
    10681 catccgataa ttgattgtat ctttcatctt ctttaactat ttcacggaaa taattgtatg
    10741 aaaaatcatg cattttcttt ccgtccccat gttcatcaat gtacaatgcc atgtaaagac
    10801 tatgatgctt ttttgcaaag tagatatagt tcaaaccgag atcaatgatt ttatcaccag
    10861 tacgttcaat agggaaaaca tcttctttta gttgttcaaa gatagaatcc agcaacgtat
    10921 tttttaaatc ttccatgttt ttgaattcca gataaatcgg ctgtgtcgaa atccccatct
    10981 ttttagcaat atttctcgca gtaaatcctt taaacccttc ctttgcaaca acatcataag
    11041 cagcttttag aatttgatcc ttggtataga cttttcttct cattaaaact cccccacttc
    11101 tttactgcag tatatgttgt tttattgtac cctattttta gcattttttg caaacatttt
    11161 actcaaaacg ttatatttta aatttttttc agtcttcttt aacttttttt tgcaaaatta
    11221 aaaaaatttg gttaaattca atgggttccc ttacattttt agttttgacc taaatgatag
    11281 ttgtccaaat ctttcatttc aagttatagt taaagaaaat gaaaaagaag gtgtgtaatg
    11341 gaaaacgaaa ctgacgttat ttatattcat cctcaaaaac gaatcgtttc acagaaaaga
    11401 aagtattttt atcttggttt tacaggtgtt ttttttcttt ttattggctt gctttctaat
    11461 actcctactg ataattggag tggattgcta actattctta cctcgccaag caatcttttg
    11521 acagattatt ttgctttagg tgttttcgga agcgcattca taaatgtagg gattttgacc
    11581 ttactttctg tgcttttagc ttatcggcac aaggtgattt taaacggtcc cttatttgct
    11641 tcgattctaa cagttaccgg tttctctttt tttggcaaaa acctttataa ttctatctca
    11701 atcattttag gtgtgtatct atacgctgta tttgtcaata aacctttttc tcagtacatc
    11761 atgatcggac tgtttggtag cgtattatca ccggtcgtta gttatatcac ttttggtatg
    11821 cgttttcctt tactgatagg tattttattg gggaatttag ctggcatagc aattggtata
    11881 cttcttccgc ccttagctgc tcaaacttta gttttccatc gtggattcac actttataat
    11941 attggattca cctctgggtt gattgcgatg acttttactg ctgttttgag gttattcagc
    12001 tattcaatcg tcgaaaatac attggttttc aatgaatatc actttcctct catttggatt
    12061 atttttggct ttttttcgct gacagtcgga attggtttct attataattc ctttcggctt
    12121 tcggggattc gagaaatttt cgattcatct ggaaaattga caactgattt cattgctaat
    12181 tctggtattg gtgctacttt gatcaacatg ggcttagttg gtctgatgct gtcttcttat
    12241 gttttgctgg ttggcggtca gttaaatgga cctgtcatcg gtgctatttt atctgcggtg
    12301 ggtttcagtg cttttggttg tcatttaaaa aacagttttc cgatactagt cggaatattt
    12361 attgcttctt tgtttggaac ttttcatgag atcaccagta ctggcatgct tgttgcagct
    12421 gtgtttggga caggtcttgc gcccataagc ggtttttacg gcagttttta cggtgtaata
    12481 gctggtgtgc tacatattgc tcttgtccat aacgtcagca ctttgcacgg aggactaaat
    12541 ctatataaca gtggtttttc aactggtttc gttgcaggta tccttgtacc gatactagat
    12601 aactttaccg ctgtcagaaa ggaaaaaaag acacttggaa aaagaattat caaaaagaat
    12661 catcgatgag cttcttactt atttatattc ccatgaaata actgaaatac gtattggagt
    12721 agaatttgct gaggaagggc ttttcataga gatacagggt acggcagaaa ctcagccaga
    12781 agatttagat gtacttgctg atttgctgaa caatcctcgg gaccctagta tggaaatcta
    12841 ctatcaagaa ctattaggta atccccgaca tgatcgggaa gattatcatc tgttaggtag
    12901 tttgattgat gacgtggaaa tcatgtatgc ccagccgatt atcaatatta agatctttcg
    12961 acgtgcttct cattcctatt attaacccta ttttctattt ataaaagact gaatcaaaaa
    13021 agcagctcct gaaagttcga ttaaaatcta ctttcaggag ctgttcaagc ttgacattat
    13081 tgattttcca atccttcata cacttcatct gttacaggtt ctaaaaactc tgaattacca
    13141 gcagtgatcg ccaaatgttc aaaccaactg tcttttgtcg caccatgcca atgtttaatc
    13201 cctttgtgcg ttacgacgac atctccagcc attaatcgtc ttgctggctt tccttcttcc
    13261 tgataccagc cttctcctcc ggtcacaagt aagatttgat agccgtctgt atggacatgc
    13321 cagtgattgc gacaacctgg ttcaaaagtt acattaccta caccaacaga aacttcggga
    13381 tcattcacta acatattcag atagctttgc ccggtaaata cttgagcgta agcttcgttt
    13441 ttttcacctg ctggaaaaat cacgccattg tgtatttctt cgtgttttgt catcatttac
    13501 gcctctttct tatcttcgct attttcttct aaatagattt cttttgctaa actaaaagct
    13561 gaccaagctt ttggccattc tacataaaaa gctaaatggg taatgatttc tgctatttct
    13621 tcactagtaa tgccattttg cttgccaatc ttcagatgag ctggcaattg ttcaaaattt
    13681 cctcctgaaa ttaatgcaga tatggtgatg atactacgct gatgagcaga taattcttct
    13741 ctggaccata cttgtccaaa taaaacatca tcatttaatt ctgcaaattt tggtgcaaaa
    13801 tctcctaatt gatctcgtcc tgcggtttgt ttttctgcca tttctcttat tctcccttca
    13861 cgactgttag gtgattcaac tatggttcta aggcaacttt attttctatt gtttcttcgc
    13921 atttcatttt aaatgtagtc tttcagcctg tccaatgctt agtttattca gtcgatatgc
    13981 tttttaagca tagttgctaa cgcttttttt attttaaaaa aaacaagtta ttaaataagt
    14041 ctttagttgg atgttttttt gagatcaatc ttttatactt atgatgaact tacaaaattg
    14101 tttgttttct gtcatatcaa aaaatggcgc agttagttgc ttttctagta aagtatagtt
    14161 aacaaagaac ctctaaggag ttgatgaata atgaatattg ctgcctctcg aaaactgata
    14221 aatttcatat ccattatcgg attaggccta tcaatcgcgc tgacgattta ttttattaat
    14281 ttaggtgttt tcaaagattt gaatgcgtta agaggattag taggtgattc catcatttta
    14341 ggaccgatta tttttgtctt catccaaatc ctgcaagtag ttattccaat cattcctgga
    14401 gggatcagta ctgcagctgg tgtcctgata tttggccctt atgctggatt tatctataac
    14461 tatattggaa tctgcattgg ttcgattatc atttttctgc tagggcgtcg ttatggaaag
    14521 ccattcatac tttccatgat tagcgataag acatataaca aatatattgg ctggctggat
    14581 aatcaaaacc gttttgagaa gctgtttgct ctagctattt ttcttccagt tgctcctgac
    14641 gatgcgttat gtttgatggc tggattgaca aacatgtcag tgaaacgata tacactaatc
    14701 attttgattg ctaaaccact gtcgattttc ttatatagta tggccttgat ttatggaggt
    14761 caatatctaa gtggtcttct tggataatat ttattttgta aaattagtca ttgattgaca
    14821 atgt
//
LOCUS       DAZUMO010000068        14815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000068 DAZUMO010000000
VERSION     DAZUMO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.068"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8ER_002172"
     CDS             complement(296..1024)
                     /locus_tag="ACG8ER_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050738.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8ER_002173"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8ER_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5050739.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8ER_002174"
     CDS             2805..3743
                     /locus_tag="ACG8ER_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050740.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8ER_002175"
     CDS             3755..4666
                     /locus_tag="ACG8ER_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5050741.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8ER_002176"
     CDS             4690..6162
                     /locus_tag="ACG8ER_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5050742.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG8ER_002177"
     CDS             6213..6824
                     /locus_tag="ACG8ER_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5050743.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG8ER_002178"
     CDS             6854..8767
                     /locus_tag="ACG8ER_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS5050744.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG8ER_002179"
     CDS             8895..11876
                     /locus_tag="ACG8ER_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS5050745.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG8ER_002180"
     CDS             11873..13534
                     /locus_tag="ACG8ER_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS5050746.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG8ER_002181"
     CDS             13574..14506
                     /locus_tag="ACG8ER_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5050747.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZUMO010000069        14048 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000069 DAZUMO010000000
VERSION     DAZUMO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.069"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..177)
                     /locus_tag="ACG8ER_002182"
     CDS             complement(<1..177)
                     /locus_tag="ACG8ER_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5050748.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLAS"
     gene            complement(656..1375)
                     /locus_tag="ACG8ER_002183"
     CDS             complement(656..1375)
                     /locus_tag="ACG8ER_002183"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5050749.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1424..3070)
                     /locus_tag="ACG8ER_002184"
     CDS             complement(1424..3070)
                     /locus_tag="ACG8ER_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5050750.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3249..3839
                     /locus_tag="ACG8ER_002185"
     CDS             3249..3839
                     /locus_tag="ACG8ER_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5050751.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3931..5436
                     /locus_tag="ACG8ER_002186"
     CDS             3931..5436
                     /locus_tag="ACG8ER_002186"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS5050752.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5520..6872)
                     /gene="celB"
                     /locus_tag="ACG8ER_002187"
     CDS             complement(5520..6872)
                     /gene="celB"
                     /locus_tag="ACG8ER_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5050753.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6872..7390)
                     /locus_tag="ACG8ER_002188"
     CDS             complement(6872..7390)
                     /locus_tag="ACG8ER_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050754.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7406..7732)
                     /locus_tag="ACG8ER_002189"
     CDS             complement(7406..7732)
                     /locus_tag="ACG8ER_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS5050755.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7745..9724)
                     /locus_tag="ACG8ER_002190"
     CDS             complement(7745..9724)
                     /locus_tag="ACG8ER_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5050756.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9745..10059)
                     /locus_tag="ACG8ER_002191"
     CDS             complement(9745..10059)
                     /locus_tag="ACG8ER_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS5050757.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10227..10520
                     /locus_tag="ACG8ER_002192"
     CDS             10227..10520
                     /locus_tag="ACG8ER_002192"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS5050758.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10598..11710)
                     /locus_tag="ACG8ER_002193"
     CDS             complement(10598..11710)
                     /locus_tag="ACG8ER_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5050759.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11863..12711
                     /locus_tag="ACG8ER_002194"
                     /pseudo
     CDS             11863..12711
                     /locus_tag="ACG8ER_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13064..13813)
                     /locus_tag="ACG8ER_002195"
     CDS             complement(13064..13813)
                     /locus_tag="ACG8ER_002195"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5050760.1"
                     /translation="MVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(13886..13948)
                     /locus_tag="ACG8ER_002196"
                     /pseudo
     CDS             complement(13886..13948)
                     /locus_tag="ACG8ER_002196"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4363 a         2880 c         2411 g         4394 t
ORIGIN      
        1 gctagccaag aacccatctg ctgaaatatg aaaatttttg aatgctacga cattcgccat
       61 tacaggtatg taaaagaaaa aaatcatcca aagcataccc ggtacagcca tcaggacaag
      121 tgctttataa cgccatagat tcataaaaaa atgattgaac ccctttcgtt ttttcatcta
      181 aaaacccctt tcgattcatt cgcaacgtaa ttatagcgct ttcaataatg agaataaata
      241 aacaaagtat agggaaaatg aaacaaagta taggagaggt tgtgaaatca gcgttttggc
      301 ctgagtatta gagaaaaaaa cgggaaaata gttcctcata ttttgccgtt ttttgagcta
      361 ataccgaggg tcagctgatt gaacaccgtt tatcagaggt tgtgaattca gcgttttggc
      421 ctgagtatta gggagatcgt gtccttacta tctaactctg agtcacaagg aacaatgggg
      481 actgtaacaa aagtcttgtc acagtccctt attccgaaca aacggtgttc cagaagtagc
      541 ttctacggaa ataagccgaa atttcacaaa aaagccatcc gaaagtcaga cggacaagtc
      601 tgcttttcag atggcgctac aactactctg cttattcttt tagctatcca ttcaattatt
      661 tttctctcac ttgttctagc tcttctttgg tcatgggtcg atattgccct ttattcaaat
      721 ctttaggcag ccaaaatttt cccatacgga ttctttttaa atataagact tcttttccta
      781 ctgctttgac cattcgctta acttggtgaa actttccttc atgcaaaatc agacggataa
      841 ttgaagtttc tgtctcttga tctacttgat caatttggag ttttgccggc aatgtctgct
      901 catcgccatc aataatcagt ccttctgcaa ataaacggat atcttcttct gtcattatac
      961 cgctaacctc agctagatat tctttctcca catgtttttt aggtgatagt aactgatggg
     1021 caaacacccc gtcattcgtt aaaatcaaga ggccctctgt gtctttatct aatcggccga
     1081 ctggaaaaag atcttcccga taatcctggt cttccaataa atcgattacg gtttgatcat
     1141 gattatcctg agtagcagaa atgactcctg caggtttatg caaaatgtag taaaaatctt
     1201 tttgatacgt aatgatttct cctaaataag taaccgcatc ttcgaattca tttacttgcg
     1261 ctttatcgct tttcaccact tgtccattga cttcgatcgt tccttttttg atcaatcctt
     1321 tgacttcttt gcggctgcca agtcccatat ctgctaaaaa cttatctaaa cgcatgcgtt
     1381 tcccccattt caaaagagca tcaatctagc tcttctgatt cacttatttt atttttaatt
     1441 tatgtcgtaa acgcgctatt cctggtccta gtagtctttc tgccaagcgg attttcaaag
     1501 ctaaataacc atacactaaa gcccctatac ctgctactac aagaatcaac aatagagact
     1561 ggaatttgct atcttcgttt aaaaaagcac caaaaatcat ctttgtgatc cacgaagcca
     1621 aaagcataat caaggtaaga ataaagatca gcaatgtcct gcgccagaca aaacttggtt
     1681 taaagcgggc tacttcatgg attctcttta agatcaaggt acaagacaca gcaaatccga
     1741 tcattgtcgc caataaaggg ccgtatactt caaataaacg aatggctggg tattgaagaa
     1801 ggagtttcac tagaaaccca ataaccaaat aattaattgc cgccttgttt tcaaacattc
     1861 cttgcagcat gttggaaact agcatatata gtcctaggaa gagtcctaca aaacttgctt
     1921 ggatcagtac attacttcct agagaatccg gtgtataaaa taatgtattt agcggatagg
     1981 caagtaacat aacaccaaat gtcgccggaa acataaaaaa tgaaaacaac tgtaaatttc
     2041 cgcttgtcaa ttttgccaga cctactcgat ctttcaacgt aacagcttct gtaatcaacg
     2101 gtaacccagt agacgcgatc gatgttgcca atgcaataac taccatcgtc aacttatctg
     2161 gattggcact aaaaatcgaa aaaagatcca gtaactgtgc attggaatac tcggtggatg
     2221 cagacatgat cttcataaaa gtgaactgat cgactaattt gaagatcgtt acccctgaac
     2281 ccacaataat aaacggaatc gcttctttaa cggtatctaa caacaattcc ttcgttgcaa
     2341 tatttatttt gttttcgctg tagtgaataa acgccgatgt atacgcttga tgtttcttca
     2401 aaaaatacaa aaggacaaga atacttgcta gcataccgat aaatgccgcg aaagttgatt
     2461 gcgtgaccgc cgtcacatag tcaccatcca tgactttcat gatgatgaat gtagccaata
     2521 gcatataaaa aatacgtgcc acctgttcta caatctgcga cagcgcataa ggcatcattt
     2581 cttgattgcc ttgaaaatac cctcgtatca cactcataca aggaaaaacc aatacagcga
     2641 tactcaaaga acgcatcgtt ggaatcagtt cttctccgcc accagaagca tgagctagcc
     2701 aaggagaggc gatgtacatg aacaaagcaa acaacaggcc tagcccgccc atcatctgta
     2761 atgcacggat aaataatcgt cgtgaagtgc catattcatt caatgaatta tagcgtgcag
     2821 tttgttttgc aatcgcagca ggtattcctg ctgtagagat cagtagaaac aatgcatata
     2881 tattgtatcc catattgaat aatccattgg cagccttagc attttcgccc atccaagcat
     2941 accagggaat gatatagaca gcgcctagca atcttgaaat gatattactg gcggtcatcc
     3001 aagcagagcc tcgtgccatt ttttcttgcg gtgttaattg atctaatgct ggttttcttt
     3061 gattatccat cggtcagatt ccaactttct acaaaacatt actatcattt taattggtaa
     3121 aacagaaact ggcaatcgat tttatataac ttttataaga acaattgttt ttttgaattc
     3181 ggtttcgttt ttttggtaaa gtaaagaaat atacagcctt ttatggctaa catttatagg
     3241 agatatcaat gacacaagaa ataaaaaaac tacccgttat atttttaggt cttttcttta
     3301 tcacagttgg attgaattgg ttcttgctcc ctcacgatat agcttccgcc ggagtgggcg
     3361 ccatcggaca tttagttgaa acggcctttt ctatccaacg ctcagttact gtttggagca
     3421 tcaatcttat gatgctgctt ctagccgctg tcctattagg aaaatcagta ttttttaaga
     3481 ctgtagcggg aagtctgctt tttcctgttg ttttagagat tgttccaaaa gtggaaatga
     3541 tctcctcaca tttcttttca ctgatttcag gaagtctttt gttcagtctt ggggtttaca
     3601 cattgtacac agtcggggcc tcaaatggcg gtataacgat accgccgatt attttccaca
     3661 aattttttcg gcttccaatg ccgcaaggac ttttacttac caatagtctt atcgtctttt
     3721 tgaattatta tgtttttgga ttgttggaga cactattcgt tctcctatct attacagtca
     3781 gttcattgtt tatgaaaata ctcatccgct tgtcccctgt gtcaagaaaa tttgaataaa
     3841 gctggatttt gtcatagcgg aaacaaacga tatattaaaa agagtgtgct ataataaacc
     3901 aagaaattga ataaagcgag ggatcaatca atgagcttct ctttagaaga catccgtcat
     3961 ttactattaa aagaaaatct attaaaagaa tttgtttcaa caagaggctg gcatcttgat
     4021 gtaccagaaa aagctgaatt tcttcagtta agttatgatt caagaaaagc tgatgcttct
     4081 actttgtttt tctgtaaagg aatgaatttc aaagaagaat atttggattc agcaatcgaa
     4141 cagggtattc aatattatgt atcagaacaa ccatacgaaa attcagcagt aggaatcatc
     4201 gtaacggata tccgtaaagc aatggcgctt cttgctatgg ctttttacga ctatccgcaa
     4261 aataaattga aagtgatcgg attcactgga acaaaaggta aaacaactgc tgcgtacttt
     4321 acaaaagcaa tcttggatca cacaacaaat aaaaaaacag cattgctttc tactatgaat
     4381 acaacgttag atggcaaaac atatttcaaa tcccatttga caacgcctga gtcattagat
     4441 ttatatcgta tgatgtcaga agcagttgaa aatggtatga ctcatctagt gatggaagtt
     4501 tcttcacaag cttacaaaac acaacgcgta tacggcttga ctttagatgt cggaatcttc
     4561 ttgaatatct cgcctgacca tatcagtccg atcgaacatc cgacatttga tgactatttc
     4621 tattgcaagc gacaattgat cttgaattca aaaacagttg ttttaaatca tgaaagtgac
     4681 tattttgatt tacttaggga aacagccgat ctattccaga ttccaacgat cacttatggt
     4741 cgttcggaag aaagcaatta tcaagtgatc cgttcagata aaggtactca cggttttact
     4801 ttatcttctc atgaagatcg cttagctatc tgcgatactg cttatgatat tttgctggct
     4861 ggaggattca accaagaaaa tgcagccagt gcgatcattg ccgcagcact aacaggtgcc
     4921 tccagtcaag atgcacaaga cggattaaaa gaagctcgtg taccaggccg aatggatcaa
     4981 ttgatccaat ccaatggagc gcatgtctat gtggactatg cacataatta tctaagcttg
     5041 aaaacattgc tggaatttgc caaaaatgaa catccagatg gccgtgtaat tgttgtatta
     5101 ggcagcccag gaaacaaagc aatctcccgt cgacatgatt ttggaaaagt gctatcagaa
     5161 acagctgaca tcgccttttt gacggcagat gacccagcat ttgaagatcc gaaaaaaatc
     5221 gcggaagaaa tcaatgaagc aattactaat ccagatttga tcgttcatta tgaaatggat
     5281 cgtcctgaag cgattcgacg cgctttagca gaaagcgctc cgcaagattc agtcgttatt
     5341 gcgggaaaag gcgtggatcc ctatcaaaaa atcaatggtg tagatgaacc ttatgaaggg
     5401 gattatgcaa tcgtgaagcg gttgattgaa gaataatggt atttatatac tagtggtttc
     5461 ccatttctaa gaaaaaaagg gactgtgatt ttttgtcaca gttccttttt tcttagaaat
     5521 cattttaact tttcactagc taattctgct gccttttctc tttcttcatt taataattta
     5581 ttatcataga atttgataaa tgggaaccag ataatgaatg cgatgattgc acaaatgact
     5641 gctaacacag cagctttcca atcaccacca ctaccaacaa atgctccgat cccgactggt
     5701 gaaggccacg gcatctgtgc aagcatcggt ttgacgattt ccagccgaat ggcaaaataa
     5761 gcgagtgtag cagaagccat cggagctaag aaaaacggaa ccgctaaata gggattgtag
     5821 acgattggca tcccgaaaat gattggttca ttgatattga ataatcctgg tacaagtgag
     5881 gcttttccta acactttcag ctgatcagat ttcgcgagga atgcaataaa gatgatcaat
     5941 cctaaagtcg cacctgatcc accaacggtt acataagaat tgttgaattc tcctgccaac
     6001 ggataatttg cgccttgtgc gttagctgcc atattagcta atacgatcgg ggtcaagaaa
     6061 gaagtgatga tattcgctcc atggatccct acgatccaca atgcatggat caagaaataa
     6121 atcaccatga caccaagcca tgtattcgtc aactgcgtaa caaacccaaa tgggatagca
     6181 atcattttga agatatccgt tcctagtgca actaataatc cattgatgat catcaccaca
     6241 aacgcaatga caaatgttgg gatcagagca gtgaaagaac gagaaactcc tggcggaaca
     6301 acatctggca tttttatgac ccaatttctc ttgacacaca tacaatacaa ttcgaccgca
     6361 ataatcgaca taataatagc tgtaaagatt cctgaagttc ctaaacgttc gacaaaagag
     6421 cccatcctaa taccatttac tacagtttct gtctcattga tagaagcaat caagctgatt
     6481 ttaccatcag tcatgatcaa ttcagggata cacataaaga atgcaaaaac agatagtaat
     6541 gcccccgtga gcggattaac atttaatttt tgttcatctc gttcaattga cgttaattca
     6601 taagcaaaca caatggcaaa gtaaatagat aaaatcccca tcgtcattgt attgacgatc
     6661 atgtacaagt cactaacttt gaaaaaagtt gcattaaaaa atccttctaa aaatgggaac
     6721 ggtaatggca aaatatttaa aaccaaaaac atcgagccta caatcgtgaa aggaatagaa
     6781 gccatccccg ctgccatgac agcccgaacg attttaagtt gcgcaatttt ccccataggt
     6841 cccatcaggt atttctgcaa aaaatcaaac attagttatt ctcctttcct aaatataacg
     6901 ctttttcata ttgtttaata cggatatact gttggtcttg gttgagtgaa attttcttta
     6961 aacgatactg tataaatgca ctggcaaaaa taccgcttac aacaagaatg aaaacaaata
     7021 actgtcctcc attgttattt gccataaaag gatacaagaa tgtaaatcct gaggtaaaca
     7081 acactggaat cactaataaa ttaaagacac tctggaacaa aaaataccac tttgtccatg
     7141 gaacgttcgt ttggtgtttt cgataaagct ttacttgttc tcctactgca ggcaaaatag
     7201 ctaaaatcag aatcatcggt aaactacttc ctaaactttt agctgataga agcagaatca
     7261 tccattgcat gttcatgaaa aagaaaaagg cagtcaggta acgtacgatt agataacgat
     7321 tgaaatacat gtttttcaaa ctaagttgct ttttttctaa ttcgattttc ctttttactg
     7381 tatcctgcac aatgatcctt cctttctatt tatttttttg atacaaataa ttcatttcct
     7441 gtgcaacttc caataatgtc atcgtcgtca tcagatgatc ttgtgcatgg accatgataa
     7501 tttccatttc tatttttgtc ccgctggcat attctttcag aagttgagtt tgtgactgat
     7561 gcgcatgaag aatctcttca tttgcttgtc gcaacttctc ttctacttct tcaaacatct
     7621 ctttacgcat cgagcgaaat gcttcatgta tcagtgtccg tgcatttccg ctatggaaaa
     7681 tgatatcaaa agaagtcact tgtagtgttt cactgtttat tttttcagcc atctgttttc
     7741 ctccttattc gatcagtttg ataaatttac tgctgaaatt ctcaaaaata ggttcagcta
     7801 agatttcctg ctgtactttt agctggtcaa ctaacctaac gattgcttta gtcacgaccg
     7861 taattccttc attttcgaca taagaaggcg aaactaaaaa aacaaaatga atttcttgat
     7921 attgatccca ctgcatccca gaaggaatca atgcaacggc tacctttgtt tcgattccaa
     7981 ctggaacagc gggatgtggg actgccactg tatcactgaa tatgatttgt cccatatttt
     8041 cacgttgctg gatctgcttt cgcatttctc ttatatatcc ttcttcttca tttatgctca
     8101 gcttttgcag caaatcatga agaatttctt cctttttcgg ttcgtacgta taagtcttga
     8161 aaaatttttc agacaactgt tcggtgtaaa tctgcttttt ttgctgtaga gaaaatgctg
     8221 gtatcggtct atctgaggaa tgatagggta tagattctcc aactaccttt cgtattttac
     8281 gaacatcatc ttcattcaaa aaaatactga catgtaaaac tggaattttg aaaaacattg
     8341 tcgaaagatc aatagaagaa ataatcaggt cgattccttt taaagttgtt tcattgatct
     8401 cataatagcc ttttacactt accactgtaa tatttttacc gaattcactg actacgcgat
     8461 tctttaatag ttgggcactg ccgtaacccg ttgcacagat aatcaatgca tgtagtttat
     8521 gcgcagctct ttctttttcc aatgccgcca tgagatgcaa agccaaatag gcccattcat
     8581 cttcattgat ggtatagttt ttcaaagccg gcatgttgct gaaatgcctt tttactgcgt
     8641 caaagacttt gggattttct ttctttatct cctcagtcaa tgggttctca atgcgaatac
     8701 ctcggctcaa tcgaatcatc atcggtgaca aatgatcgag tagtccattg aaaagctgga
     8761 gatcctctac taatgaaaaa tgaaattcct gagacaatct tttcagaact ttcgctaatt
     8821 cctttgatag ttcctgatca ttttgatttt ctttgtgatt cgacttagcc atgagatgta
     8881 atgtcaaata agcaatttct tcttcgggaa acgatacacc tgcgattacc tcaatacgtt
     8941 gaacgatccg ctgcgccact tgatactcta ctcgcttaga aacttcttgt tcgatgccta
     9001 attccttgat ttcaaaccct tctcggagcc gtttgatccc caaagccaag tgtaaagtca
     9061 gattctgtat gataaaatcc gaaactttta acttagcttc tcttgtttca tcgagaatga
     9121 taatgactag ctcttcaaaa ctgatgtctg ggaaaaaacg gctttgccct aaatattctt
     9181 tcaatgaatt tccataatgg tttccaaaaa aagtatccat gataaaatgc cgtcgatttc
     9241 tttcttcccc ttcgatccat gtcccttgac catgcttaga aacgattttt agtgaatatg
     9301 gaatcaatcg ctccttgatt tcttgcatgt cctttgtcaa actggaacga ctgatataaa
     9361 gctcttctgc caaatcatcc ataaaaagaa tggcatcttc caaaagcaat tggttcaata
     9421 tatacttttg tcgatcttcg gactcataaa atccttgcgg ttctctagca tatccaggta
     9481 ctatttcttg ccgactcaaa aacaagtcaa atgtcagtct ttgtaaaata tgcagttggt
     9541 agccataacc tggtttagca atgatcttac ctccgttttt ttctactaac tctttgatcc
     9601 gctgcaagta attacggatt gtccgatcag ataataacaa ttcagaagct aaacgttgac
     9661 tggtgacata ctccttcttg tgatcaatca aatatctcaa cagcaatttt tccttctgtt
     9721 tcattatcat ccactcctat tccatcatat gttctcttcg atcacctctg ctaacttctg
     9781 gatgcctgta gggattggga catacgcttg gaaagggata ctaacaacag gctttccaac
     9841 agattctcct aattttttga atttatccaa aaacatcgtc gtttgcgggc tgatcagaaa
     9901 taaatcaaaa tcactattct taatcatatt atcgccttct gtagctgaaa ctgcatctac
     9961 gagtacctct tctctttttt gcttaaagaa atccgttgtc tttgaggcca tcatcgagga
    10021 tgacattcct gccgcacaaa taatcaatgc ttttttcata aatcaatcac tccttttatt
    10081 tttataaacc aagcatatct tgtaagcgtt ttctattcaa aatatatatt tccgtagcat
    10141 tgcggaaaaa atgtgtatca tatcttgaat aataatttct ggcaccgtga tatactagtg
    10201 atagaatttt ttcaggaggg gatttagtga gtatcacagt gaatattttg tatacaagta
    10261 caaatggaca agccagaaaa tttgcagaag aaatggtcga aagaaacatt gtcaaagcga
    10321 ttcgtgcaga agaaggaaac gagaaatatg actattactt tccactagaa gatccagaat
    10381 ctcttttact gatcgaccgt tggaaagatg aagaagcaat cgaaaagcat cacaaatccg
    10441 aaatgatggc tcaaataaac gaactccgta aaaagtataa attaaaaatg aaagtcgaac
    10501 gtttcactga tttaccgtaa aaagatacac atggaatgat agcaatttat catccatgtg
    10561 tatcttcact ttttccttat tttttattcg atctcatcta atgattcaaa ccgtgttttt
    10621 ctcttatttt tcggataagt atttcagtca ccccaatata catgattcct aaacataccc
    10681 caaggatatt ctcaaaaatg cggatcgtta ctgctcctgg aaccccgaat aaactggcag
    10741 tgattgccaa agcaccaaaa gaattaaaaa ctgttttata tctataggta gaacaaacac
    10801 ctaaagctag accacctaat ataccgaccc agttaaaagg aataaattgg ctgataccaa
    10861 taaataaaac acatccaatc aatgagccaa tgattcggtc tactgctcgt tctttgaaac
    10921 caatcgacat caatccatag ctagatacaa tagaagaaaa tgcaaaccct gcccacatca
    10981 atcgctgaaa agggacatac tctcctgcaa cgatcaaaag actaatgcca aatgcataga
    11041 tgagcatcca aattttttcc tgcgaataca tgcctttcaa aaaaatctct ttaaacaagc
    11101 ttttgtcttt gttcttttct cgatgttttc tgtaaagaag aactgaaaac caacaaaaaa
    11161 acaaaaataa taagctactt gtttgtgtaa agaaattctt gttcaacgaa tctttaggta
    11221 acgaataaac aataaagaga tatgaaaagc cgtacaaaga agcattcccc attttgggat
    11281 cactagcggt tgccattaat atgctgctta gtaaaataaa atgaatcaac atgcccataa
    11341 ctgaccattt gatcagctgg acatacggtg ctactaatag tgagaacatg acgattgcta
    11401 aacctatgat cgaatgggaa atcttataac caaagtcaac aaaacgaata ctcatcaaca
    11461 tacagaataa aaccacagcg tatggtttat gcttcgattc aaataatgtt gtaattataa
    11521 tgataaaaca aatggcaaac aatacgatga ataatgatcg taaaaacaat gaacaaatat
    11581 acatgcactt ttctttttta gaagctgctt ctcgtatttt ttgttttaaa ataaacggat
    11641 ctagttgtag agactgataa aaatccacct gttgtcgtcc tttcttttta cttttatttt
    11701 ttacagtcat ctatgacagg ttgtaaatac tatggaacat catatggcgt tgtcattttc
    11761 ttgtcaacta tttttcctat tcatgttttt ataaaaaaac atatcgtttt cttgtatgat
    11821 taaatcacca caaaataaac aactgagaga acgatatgct ttatgaatgg ttctatcaaa
    11881 aaaattctga gatcaataga caaaaatttg atgattacag aggtctctta cgaaacctta
    11941 cagaagaaaa aaacattgat cgttgctgca gttctcttcc ctgctccatg tgtctgtaga
    12001 aattgtggtt ctgctgtggt agatggaaat aggaaattaa tggtggtgaa aaacaagaag
    12061 aaggaaacca ttgtccactt tgaataatat aatcatatgc caatggttat acgcctaaaa
    12121 aagcagcgct atacctgtaa aaactgtcga agccattgga acgctcaaag ttattttatc
    12181 cgtccaaggc actcgatttc aaatcatgtc agacataaaa tcacttcttt actgactgaa
    12241 aaagtgtcgt tattttttat ttcgaaaagt tgttaggtat ctttgacaac cgttattcgc
    12301 acattgaaag agttgagaag ctatttacca aagtcttcca aaagaattct gtcttgagtg
    12361 ttgagggttg tgaatttcgt tctcacgttt ccgttgagga taaactgagt tttatttccg
    12421 cggatggcga gacagaaaaa ttaatcgaga aaatttacct acacaaaaaa ttcctcgatt
    12481 aacaaactat ttccttagct gcactaatcc taaagaaata gaattcttag ttacagacat
    12541 gaacgccacc tactttcagc tcaccaaaca tgttctacca aatgcgaaat tggtgattga
    12601 tcgattactt ggtttttcca cgcctttcaa ggaggcgtaa cctttattca cgagttggtt
    12661 gaagctttcc gagacaaaag acccgacttg tttttctcct tattgacaaa gagccattaa
    12721 aactagagaa aaaattacaa ctgatcgctg attatttgta gaaagaaaat caaagtatta
    12781 cccaataaac ttgagtcatt aggcaaaaaa cactgcgaaa ctcaagaatg attttcgcag
    12841 tgtttttgta tattttttat taactagtat tactacaaag gtttattttt cacttctcaa
    12901 cgcagcacaa aattgtcttt acttatttac tggttgggtt caaccttctc tactccgata
    12961 ctaatatttg tttgtatacc ccttctaaaa tcggtagttt ccattcaatt gatgggtact
    13021 agttgatttt aaaaactatg cttattcaat tttttatttt agctcatata tttcgtttct
    13081 ttctaaattt tatactgatg aaaacaagta gcacacccaa tattgacaat ataaactggc
    13141 taatgatggt ttctccagtt tttggaagat gcttatttcc actgcttgtt ggattgtttc
    13201 ctcttatatc tgagctatca gcaacttttc tattctcttt tgttaaaaag atcgccactt
    13261 cttgttcttt ttcgatcttg aaagttactg gtgtcgcatc caattcatac cccgttggcg
    13321 cttgagtttc taccagctga tatgctcctg gtgctaagcc atctatcgcc agttttccat
    13381 ctgctcctgt ggttagacct gactgcagaa cggttccatt ggcatcttgt aactcaaaaa
    13441 cagctccttg caggacttct ccgctttggt catcgatctt tgtcaacacg acaccgcctg
    13501 gtgtaagtcg attttctttc gtcaattcta ccaccgctgt ctgacttctt tcgatctcaa
    13561 actcgattgg tgtcgcatcc aattcatacc ccgttggcgc ttgggtttct accagctgat
    13621 acactcctgg tgctaagccg tcaatcgcca gttttccatc tgctcctgtg gttagacctg
    13681 actgcagaac ggttccattg gcatcttgta actcaaaaac agctccttgc aggacttccc
    13741 cgctttggtc atcgatcttt gtcaacacga caccgcctgg tgtaagtcgg tttgctttcg
    13801 tcaattctac caccgctgtc tgacttcttt cgatctcaaa ctcgatcggt gtcgcatcta
    13861 attcataccc cgttggcgct tgagtttcta ccagctgata tgctcctggt gctaaaccgt
    13921 caattgccag ttttccatct tctcctgtag ttagaccggt ttgtagcgtt tctccttctc
    13981 ggttctgtaa ctcaaaaaca gctcctgcag gatttccccg ctttggtcat cgatctttgt
    14041 caacacga
//
LOCUS       DAZUMO010000070        13969 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000070 DAZUMO010000000
VERSION     DAZUMO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.070"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACG8ER_002197"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACG8ER_002197"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS5050761.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="ACG8ER_002198"
     CDS             complement(2424..2675)
                     /locus_tag="ACG8ER_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS5050762.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="ACG8ER_002199"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="ACG8ER_002199"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS5050763.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="ACG8ER_002200"
     CDS             3624..3842
                     /locus_tag="ACG8ER_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050764.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="ACG8ER_002201"
     CDS             complement(4130..4642)
                     /locus_tag="ACG8ER_002201"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS5050765.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACG8ER_002202"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACG8ER_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS5050766.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLANEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7538)
                     /locus_tag="ACG8ER_002203"
     CDS             complement(7095..7538)
                     /locus_tag="ACG8ER_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS5050767.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="ACG8ER_002204"
     CDS             complement(7539..8327)
                     /locus_tag="ACG8ER_002204"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5050768.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACG8ER_002205"
     CDS             complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACG8ER_002205"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS5050769.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="ACG8ER_002206"
     CDS             complement(9442..10029)
                     /locus_tag="ACG8ER_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS5050770.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACG8ER_002207"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACG8ER_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS5050771.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="ACG8ER_002208"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="ACG8ER_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5050772.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="ACG8ER_002209"
     CDS             complement(12946..13881)
                     /locus_tag="ACG8ER_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5050773.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3856 a         3131 c         2454 g         4528 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttgcca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZUMO010000071        13917 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000071 DAZUMO010000000
VERSION     DAZUMO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.071"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8ER_002210"
     CDS             131..505
                     /locus_tag="ACG8ER_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5050774.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8ER_002211"
     CDS             complement(619..1248)
                     /locus_tag="ACG8ER_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5050775.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8ER_002212"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8ER_002212"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG8ER_002213"
     tRNA            1668..1741
                     /locus_tag="ACG8ER_002213"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACG8ER_002214"
     CDS             complement(1879..2043)
                     /locus_tag="ACG8ER_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050776.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACG8ER_002215"
     CDS             2338..3666
                     /locus_tag="ACG8ER_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS5050777.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACG8ER_002216"
     CDS             3868..4701
                     /locus_tag="ACG8ER_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS5050778.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACG8ER_002217"
     CDS             4717..5454
                     /locus_tag="ACG8ER_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS5050779.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACG8ER_002218"
     CDS             complement(5609..6577)
                     /locus_tag="ACG8ER_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS5050780.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACG8ER_002219"
     CDS             6809..7642
                     /locus_tag="ACG8ER_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS5050781.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACG8ER_002220"
     CDS             7676..8515
                     /locus_tag="ACG8ER_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5050782.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACG8ER_002221"
     CDS             8541..9101
                     /locus_tag="ACG8ER_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5050783.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACG8ER_002222"
     CDS             9300..11021
                     /locus_tag="ACG8ER_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS5050784.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACG8ER_002223"
     CDS             11186..12328
                     /locus_tag="ACG8ER_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5050785.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8ER_002224"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8ER_002224"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS5050786.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4750 a         2298 c         2834 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattggat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAZUMO010000072        13810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000072 DAZUMO010000000
VERSION     DAZUMO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.072"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACG8ER_002225"
     CDS             complement(221..1423)
                     /locus_tag="ACG8ER_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5050787.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACG8ER_002226"
     CDS             complement(1426..1620)
                     /locus_tag="ACG8ER_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5050788.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACG8ER_002227"
     CDS             complement(1667..2515)
                     /locus_tag="ACG8ER_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS5050789.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACG8ER_002228"
     CDS             complement(2720..3070)
                     /locus_tag="ACG8ER_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050790.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACG8ER_002229"
     CDS             3248..4111
                     /locus_tag="ACG8ER_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5050791.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACG8ER_002230"
     CDS             4735..5727
                     /locus_tag="ACG8ER_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050792.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACG8ER_002231"
     CDS             5743..6627
                     /locus_tag="ACG8ER_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5050793.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACG8ER_002232"
     CDS             6676..8283
                     /locus_tag="ACG8ER_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5050794.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACG8ER_002233"
     CDS             8284..9768
                     /locus_tag="ACG8ER_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS5050795.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACG8ER_002234"
     CDS             9945..10937
                     /locus_tag="ACG8ER_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5050796.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACG8ER_002235"
     CDS             10989..11858
                     /locus_tag="ACG8ER_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5050797.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8ER_002236"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8ER_002236"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5050798.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAZUMO010000073        13433 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000073 DAZUMO010000000
VERSION     DAZUMO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.073"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..635)
                     /locus_tag="ACG8ER_002237"
     CDS             complement(93..635)
                     /locus_tag="ACG8ER_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS5050799.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1159
                     /locus_tag="ACG8ER_002238"
     CDS             770..1159
                     /locus_tag="ACG8ER_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050800.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1163..1927
                     /locus_tag="ACG8ER_002239"
     CDS             1163..1927
                     /locus_tag="ACG8ER_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5050801.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2056..3003
                     /locus_tag="ACG8ER_002240"
     CDS             2056..3003
                     /locus_tag="ACG8ER_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5050802.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3133..3873
                     /locus_tag="ACG8ER_002241"
     CDS             3133..3873
                     /locus_tag="ACG8ER_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050803.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4164..4832)
                     /locus_tag="ACG8ER_002242"
     CDS             complement(4164..4832)
                     /locus_tag="ACG8ER_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5050804.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4966..5253
                     /locus_tag="ACG8ER_002243"
     CDS             4966..5253
                     /locus_tag="ACG8ER_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050805.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5669..7207
                     /locus_tag="ACG8ER_002244"
     CDS             5669..7207
                     /locus_tag="ACG8ER_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS5050806.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7204..8169
                     /locus_tag="ACG8ER_002245"
     CDS             7204..8169
                     /locus_tag="ACG8ER_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050807.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8156..9478
                     /locus_tag="ACG8ER_002246"
     CDS             8156..9478
                     /locus_tag="ACG8ER_002246"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5050808.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9541..9645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9719..10462
                     /locus_tag="ACG8ER_002247"
     CDS             9719..10462
                     /locus_tag="ACG8ER_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050809.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10477..11469
                     /locus_tag="ACG8ER_002248"
     CDS             10477..11469
                     /locus_tag="ACG8ER_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5050810.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVNYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11481..12218
                     /locus_tag="ACG8ER_002249"
     CDS             11481..12218
                     /locus_tag="ACG8ER_002249"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050811.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12208..12930
                     /locus_tag="ACG8ER_002250"
     CDS             12208..12930
                     /locus_tag="ACG8ER_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5050812.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13014..>13433)
                     /locus_tag="ACG8ER_002251"
     CDS             complement(13014..>13433)
                     /locus_tag="ACG8ER_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5050813.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4428 a         2296 c         2788 g         3921 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1141 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1201 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1261 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1321 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1381 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1441 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1501 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1561 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1621 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1681 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1741 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1801 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1861 acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     1921 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     1981 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     2041 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     2101 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     2161 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     2221 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     2281 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     2341 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     2401 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     2461 gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac agcaaaagca
     2521 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     2581 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     2641 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     2701 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     2761 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     2821 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     2881 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     2941 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaat
     3001 taagtcttat tactatatat atatttatga aattcatcgc tattttctta aaataatcag
     3061 cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc atttttgaga
     3121 aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc gtacgatgat
     3181 gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg acaagaagat
     3241 cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca tttcacgaac
     3301 acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg
     3361 cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg ccattaccaa
     3421 tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga
     3481 caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt tgacacgaag
     3541 gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat cccagaccct
     3601 agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac ggatgactct
     3661 tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg tgagccgtct
     3721 gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa
     3781 tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga tgatcaagga
     3841 aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat attcggctac
     3901 agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa agtttatatc
     3961 gtatcctcaa atttttttga gtttaagcga attttttctc ccaataagta agaaaataat
     4021 ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt tattttttta
     4081 taaagaagat tttactgaat aatatcattt tagtttacat aatgatattt ctgtaaatta
     4141 aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca
     4201 aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac
     4261 ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt
     4321 gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt
     4381 ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct
     4441 gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc
     4501 aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt
     4561 aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa
     4621 agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg
     4681 aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta
     4741 gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg
     4801 cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt tgaaaatttt
     4861 cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat gaaaaaaaat
     4921 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
     4981 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
     5041 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
     5101 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
     5161 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
     5221 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
     5281 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
     5341 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
     5401 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
     5461 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
     5521 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
     5581 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
     5641 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
     5701 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
     5761 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
     5821 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
     5881 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     5941 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     6001 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     6061 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     6121 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     6181 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     6241 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     6301 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     6361 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     6421 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     6481 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     6541 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     6601 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     6661 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     6721 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     6781 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     6841 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     6901 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     6961 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     7021 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     7081 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     7141 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     7201 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     7261 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     7321 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     7381 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     7441 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     7501 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     7561 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     7621 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     7681 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     7741 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     7801 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     7861 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     7921 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     7981 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     8041 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     8101 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     8161 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     8221 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     8281 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     8341 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     8401 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     8461 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     8521 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     8581 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     8641 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     8701 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     8761 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     8821 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     8881 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     8941 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     9001 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     9061 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     9121 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     9181 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     9241 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     9301 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     9361 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     9421 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     9481 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     9541 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     9601 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     9661 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     9721 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     9781 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     9841 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     9901 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     9961 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
    10021 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
    10081 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
    10141 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
    10201 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
    10261 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
    10321 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
    10381 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
    10441 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
    10501 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
    10561 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
    10621 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
    10681 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
    10741 ggtgtaaatt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
    10801 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
    10861 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
    10921 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
    10981 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
    11041 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
    11101 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
    11161 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
    11221 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
    11281 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
    11341 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
    11401 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
    11461 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
    11521 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
    11581 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
    11641 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
    11701 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
    11761 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
    11821 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
    11881 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
    11941 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
    12001 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
    12061 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
    12121 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
    12181 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
    12241 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
    12301 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
    12361 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
    12421 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
    12481 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
    12541 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
    12601 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
    12661 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
    12721 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
    12781 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
    12841 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
    12901 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
    12961 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
    13021 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
    13081 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
    13141 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
    13201 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
    13261 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
    13321 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
    13381 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZUMO010000074        13429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000074 DAZUMO010000000
VERSION     DAZUMO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.074"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8ER_002252"
     CDS             complement(<1..914)
                     /locus_tag="ACG8ER_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS5050814.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8ER_002253"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8ER_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS5050815.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG8ER_002254"
     CDS             2006..2176
                     /locus_tag="ACG8ER_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050816.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACG8ER_002255"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACG8ER_002255"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS5050817.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACG8ER_002256"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACG8ER_002256"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS5050818.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACG8ER_002257"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACG8ER_002257"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS5050819.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACG8ER_002258"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACG8ER_002258"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS5050820.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACG8ER_002259"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACG8ER_002259"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS5050821.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACG8ER_002260"
     CDS             6751..7752
                     /locus_tag="ACG8ER_002260"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS5050822.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACG8ER_002261"
     CDS             7749..9110
                     /locus_tag="ACG8ER_002261"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS5050823.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACG8ER_002262"
     CDS             9070..10221
                     /locus_tag="ACG8ER_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5050824.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACG8ER_002263"
     CDS             10196..11365
                     /locus_tag="ACG8ER_002263"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5050825.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACG8ER_002264"
     CDS             11540..12919
                     /locus_tag="ACG8ER_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5050826.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACG8ER_002265"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACG8ER_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2924 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZUMO010000075        13185 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000075 DAZUMO010000000
VERSION     DAZUMO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.075"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8ER_002266"
     tRNA            12..84
                     /locus_tag="ACG8ER_002266"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8ER_002267"
     tRNA            88..160
                     /locus_tag="ACG8ER_002267"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8ER_002268"
     tRNA            168..249
                     /locus_tag="ACG8ER_002268"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8ER_002269"
     tRNA            267..339
                     /locus_tag="ACG8ER_002269"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8ER_002270"
     tRNA            351..422
                     /locus_tag="ACG8ER_002270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8ER_002271"
     tRNA            439..524
                     /locus_tag="ACG8ER_002271"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8ER_002272"
     tRNA            535..608
                     /locus_tag="ACG8ER_002272"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8ER_002273"
     tRNA            618..691
                     /locus_tag="ACG8ER_002273"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8ER_002274"
     tRNA            711..783
                     /locus_tag="ACG8ER_002274"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8ER_002275"
     tRNA            811..884
                     /locus_tag="ACG8ER_002275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8ER_002276"
     tRNA            898..971
                     /locus_tag="ACG8ER_002276"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8ER_002277"
     tRNA            995..1084
                     /locus_tag="ACG8ER_002277"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8ER_002278"
     tRNA            1097..1170
                     /locus_tag="ACG8ER_002278"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8ER_002279"
     tRNA            1189..1264
                     /locus_tag="ACG8ER_002279"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8ER_002280"
     tRNA            1266..1336
                     /locus_tag="ACG8ER_002280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8ER_002281"
     tRNA            1350..1423
                     /locus_tag="ACG8ER_002281"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8ER_002282"
     tRNA            1448..1535
                     /locus_tag="ACG8ER_002282"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8ER_002283"
     tRNA            1557..1628
                     /locus_tag="ACG8ER_002283"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8ER_002284"
     tRNA            1651..1740
                     /locus_tag="ACG8ER_002284"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8ER_002285"
     tRNA            1753..1826
                     /locus_tag="ACG8ER_002285"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8ER_002286"
     tRNA            1845..1917
                     /locus_tag="ACG8ER_002286"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8ER_002287"
     tRNA            1921..1993
                     /locus_tag="ACG8ER_002287"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8ER_002288"
     tRNA            1998..2068
                     /locus_tag="ACG8ER_002288"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8ER_002289"
     tRNA            2081..2154
                     /locus_tag="ACG8ER_002289"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8ER_002290"
     tRNA            2166..2237
                     /locus_tag="ACG8ER_002290"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8ER_002291"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8ER_002291"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS5050827.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8ER_002292"
     CDS             3826..5298
                     /locus_tag="ACG8ER_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5050828.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8ER_002293"
     CDS             5381..6169
                     /locus_tag="ACG8ER_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5050829.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8ER_002294"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8ER_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS5050830.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8ER_002295"
     CDS             7019..8731
                     /locus_tag="ACG8ER_002295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050831.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACG8ER_002296"
     CDS             8734..10503
                     /locus_tag="ACG8ER_002296"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050832.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ACG8ER_002297"
     CDS             complement(10708..11274)
                     /locus_tag="ACG8ER_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5050833.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ACG8ER_002298"
     CDS             complement(11274..12230)
                     /locus_tag="ACG8ER_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS5050834.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ACG8ER_002299"
     CDS             complement(12230..12886)
                     /locus_tag="ACG8ER_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5050835.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         3850 a         2466 c         2933 g         3936 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaa
//
LOCUS       DAZUMO010000076        13162 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000076 DAZUMO010000000
VERSION     DAZUMO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.076"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..1866
                     /gene="yfcC"
                     /locus_tag="ACG8ER_002300"
     CDS             127..1866
                     /gene="yfcC"
                     /locus_tag="ACG8ER_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS5050836.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2023..3207
                     /gene="iadA"
                     /locus_tag="ACG8ER_002301"
     CDS             2023..3207
                     /gene="iadA"
                     /locus_tag="ACG8ER_002301"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS5050837.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3318..3521
                     /locus_tag="ACG8ER_002302"
     CDS             3318..3521
                     /locus_tag="ACG8ER_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5050838.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3622..4284)
                     /locus_tag="ACG8ER_002303"
     CDS             complement(3622..4284)
                     /locus_tag="ACG8ER_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050839.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4297..5799)
                     /locus_tag="ACG8ER_002304"
     CDS             complement(4297..5799)
                     /locus_tag="ACG8ER_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050840.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6347..8026
                     /locus_tag="ACG8ER_002305"
     CDS             6347..8026
                     /locus_tag="ACG8ER_002305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5050841.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8153..8632
                     /locus_tag="ACG8ER_002306"
     CDS             8153..8632
                     /locus_tag="ACG8ER_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS5050842.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8851..9531
                     /locus_tag="ACG8ER_002307"
     CDS             8851..9531
                     /locus_tag="ACG8ER_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS5050843.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9539..10870
                     /locus_tag="ACG8ER_002308"
     CDS             9539..10870
                     /locus_tag="ACG8ER_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS5050844.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10885..11847
                     /locus_tag="ACG8ER_002309"
     CDS             10885..11847
                     /locus_tag="ACG8ER_002309"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS5050845.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11958..12296
                     /locus_tag="ACG8ER_002310"
     CDS             11958..12296
                     /locus_tag="ACG8ER_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS5050846.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12375..13082
                     /locus_tag="ACG8ER_002311"
     CDS             12375..13082
                     /locus_tag="ACG8ER_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS5050847.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4186 a         2321 c         2885 g         3770 t
ORIGIN      
        1 caaaatagaa aaaagtgatt ttctaataaa ttattcatgt ggaaatgtca tttttactgt
       61 ataattcatt tgattatcca ttttttattc atcaaaatta ctgaaaaaag aaaggaagaa
      121 ataaatttga agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac taaattatcg
      181 acaccccata cgtatgtcat tatttttggt gtagtcatct ttgcatggat actgactttt
      241 gtcgttccag caggaaagtt cagcacgcaa gatattgaat acaaagatgc aaatggcgaa
      301 acaagtactc gtactgtttt acgtcaagat tccttccgtt atgcttacga gctggataag
      361 tcgtatgtgt ttgatcaatt agaagaactg caagatcatc cagcagaacg tgaaaaactg
      421 gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa tcttacgcag
      481 gaaaaactag atgaaatctc cttgacagat gatgtgcttt acgatcagta cggcgaaaat
      541 atctatgata cttctaaaaa attgcataaa acagccaaaa tatggggaac agatgatttt
      601 ggcggctttg gtttcttaaa ttttgttttt gaaggactag tatctggaga taagtatgga
      661 tctgctgtag ggatcgctgc attgatcctt gttgttggag gtgcttttgg tattattatg
      721 cgtaccggag cgatcgacgc aggtatctat gcgtttatca gtaaaactaa gggactggag
      781 cgtttagctt taccgctatt attctttgct ttttcattcg gtggcgctac attcgggatg
      841 gcagaagagg taattccatt ctcaatggtg atggttccat ttgtcattgc gttaggatat
      901 gactcgatcg tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa tgcgacctct
      961 tggatgagcc cgtttagcgt agcggtggct caagggattg ctggaatccc cgtgttgtct
     1021 ggtgcgacat tccgattgat tatgtgggta gttgttactg cattagccgc tggttatatg
     1081 atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta caaatcagat
     1141 gattatttcc gttctcatat caaaaagaca tctgatgaaa ataaaaaatt catgcttggg
     1201 cataaattga tcttattaga gatgttagtt gtccttgtct ggatcgtttg gggcgtgact
     1261 caaaaaggct attacattcc agaaatcgct tctcaattct ttgtgatggg attagttgct
     1321 ggaatcattg ctgttctttt caaattagat ggaatgaaag tcaatgacat cgcttcttct
     1381 ttccaatctg gtgcagctga tttagctggt acagcaatcg tagtaggtat ggccaaaggg
     1441 attcttcttg tcttgggagg atctgatgca tctgttcctt cagctttgaa tacgattttg
     1501 catgggatcg ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg ggcgatgtac
     1561 atcttccaaa gtttgttcaa cttggttgtt acatcaaatt caggacaagc agctttgact
     1621 atgccaatca tggctccact ggcagattta gtaggtgtat ccagacaagt agcagttctt
     1681 gcttatcagc ttggttcagg ttttatggac gcttttacac ccgtatcagc cagcttgatt
     1741 ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg caagattcca aatcaagatg
     1801 caaggattcc tgtttgtttt aggaactatt ttcatcatga ttgccattgc aataggtctg
     1861 cagtagtact ggagacctgt aaataaaaaa gtaatccaaa cagtagataa ggactccaag
     1921 aaccgtcatt ttacggtaaa atcaaaggag taaagatcct atcgtttgga ttatttttta
     1981 agatcctatc atcagttata cttaaagaaa gaggaaaaat gtatgaaaat cattcgacaa
     2041 attgaagtgt ttgcaccaga ttacttagga aaaatggatg tactgattgc cggtgggaag
     2101 attttggctg tagaagaaca gctagaaggc ggctacgaag gagtggaagt agaagaactt
     2161 tcaggtgagg gaaaagtact aaccccgggt ttcattgact gtcatttcca tattttaggt
     2221 ggaggaggag aaggcggtta tcaaaatcga acgccagaag tcacattgag ccaattgact
     2281 acagctggtg taacgacagt tgtcggttgt ttgggaacag acggagaagg tagagatatg
     2341 actgcgttga tcagcaaagc gaaaggtctg gaagcagaag gaatctccac ttatgtatac
     2401 gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa agactttttg
     2461 acaattgata aaattatcgg aatcggagaa atcgcagtat ccgatcaccg ttcttctcag
     2521 ccaagttttg aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg catgttatcc
     2581 ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa aaagaaagct agagctattg
     2641 acacgaatcg tagaagaaac agaaattccg atcacacaat ttttgcctac tcatgcaaat
     2701 cgcacaccag aattatttga agcttgcgtg gcatttgcca aacgtggtgg cacgattgac
     2761 ttcactgcta gcgaagatcc tgatttttgg gagaagacag atggtgaagt tcgcttcagc
     2821 aaagcactga aacgattgat cgaggaagat gtttctttag ataatttcac gatgacctca
     2881 gatggacaag gaagcttgcc ttattttgat gaaaacaatc atttcctagg gttaggagta
     2941 ggaagtgcca aggcattgct agtagggatc aaagaagctg tgcaaaaaga aagtatccct
     3001 ttggaaatcg ccttacgagc tataaccagt aatccagcac gtatattgaa attggataaa
     3061 aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta tcttggataa agaaacgctt
     3121 gatattgata cagttatcgc taaaggagaa ataatggtcc aagaaaaaga agtcaaagta
     3181 tgggggactt tcgaaaaaag tttttaatca acagctctaa ctatcttttt tagaaaacga
     3241 gcgttctgtt tgtctgtatc tgttgttgat gttaccctga aggtagatca ataaacaaac
     3301 taagaggagg aagcacaatg gcagatttaa aaggacgttc taaagatatg aaagataaag
     3361 tagtcggtga aggaaaagaa gcatacggca aagcaacaga cgacaagagc aaagaaacag
     3421 aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat ttaggcgacg
     3481 aagtcaaaga caagttttct cgaaaagacg aaaaaaaata aaaaccactt attactaatt
     3541 gaatcaagca aataaaaaat ccgaaccata tctgcgagac agatatggtt cggatttttt
     3601 atttgatcat tatcctgttt tttaaacttc gattttttga aattttttat tttttagttc
     3661 aaagacttca tcacagcttt ttgctacttc aatctcatga gtcacgatga taatacagcg
     3721 attttgttca tgggcgattt cttggaaaag tttgacgata tcttgtgaag ttgtttcatc
     3781 tagatttcca gtaggctcat ccgctacgat tagttgatgt tcacagcaga tagcacgaac
     3841 gatagcgact cgctgctgct gacctcccga aagtttggtc acctgttttt ttgctaatat
     3901 ttcatcaatt cctacacgag ccagtcgatc taaagcaact tgtcgataat tctcttgctt
     3961 ggtcttagtg atttccattg ctgctatgac gttgtctaga gccgataaat aaggaagcaa
     4021 gttgtaagct tggaaaataa tcgagacatc atttttacga taacttctta acccgatttt
     4081 gttcagtgaa cgatcttgat agtatacctt tccttctttt ggtgcatcta gtccagaaat
     4141 aagcgaaaga aaagtcgttt ttccagaacc acttgctcct aaaatagcgt acatttttcc
     4201 agcttcgaaa ttcagttcta catctttata aagcgcatct tgcggatctt gataccaaaa
     4261 tcccaattgt tctgtccgta acatcaaatt cctcctttag tttaatagga tttttcttgg
     4321 gtttaatctt aatatcccta tagaagctaa aaagacagaa aacagactga ttccaaatgc
     4381 tactccggct aatagtccca attgtgcagg tttgacattg atttccaatt cgctgacttg
     4441 atcagaaaca gcaaatggat tattcgtggt tccttgttga ccatcgcgtt gaccgccttg
     4501 ttcttgtctg ttacctggca tttgtccacc gccaggagct tgctggtttt gtgtcgtggt
     4561 ttctgtctgt tggtcaagta gctgctgtcc cacggcattc gcgacgacgt ttccgctaag
     4621 cgaagcaact cctaatgcaa gaatcataca aatagccact tcggtaaaca attggagaat
     4681 gattttactt ctggattctc ctaaagatag caagacgcct aactcatgcc gacgctcccg
     4741 aatggtaatc ataatgatca gagtaagtat gataataccg gctacagtca ctagtaaaac
     4801 aacattttta gaaaaacttg ccacgttgtt taaaggttcc aacattgact gatacatgga
     4861 atcatttgat tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt ctttgagaaa
     4921 ctcatccatt ttatctggat cagatagagt gtaaatagca gaatctatcg tgttatcgtc
     4981 agatgtccca tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat tcgacggatt
     5041 catgaagttg aatttcatac ccatactgcc gcttgtttcg ctggattcat aaattccttt
     5101 gatcgtcatt tcataagtgg tatcttcatc ttcagtactc gtgattgtga aagtatctcc
     5161 gactgccagg tcatttgttt ctgctagagt agaatcaatg actacattgt ttgtgccttt
     5221 gtcgctgtca tctattcctt caccatccgt gatttttgct gttccatcag aaaaattact
     5281 attttgtgca gttgctgaca caccactgat ttgaaagtct gcttgagtca tttgcggatt
     5341 cccgcccatt cctccagcag gttcaccgcc tgcatcagta gtttctgaac tgtcttcact
     5401 tgaatcagaa ctagtgatgg cagttattcc atcttttgcc atagctgagg cggaactttc
     5461 aaaggaataa ctctttacat tatctaattc agctattttt tccgcgtccg ttaaggaaac
     5521 tggtgtaaga gaaaaactgc ctggatctgg acgttcttca gtggtagaac cgcttgaaga
     5581 agtttgttcc tgttttttaa atgaagcttc tcgattggca cttaaagtca ctgttgctcc
     5641 aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg tgaggccagc
     5701 tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac gtccccattt
     5761 tacttttgta cttttccatg cacgtttcat aaaattcata ttttgcatcc ctcctagtag
     5821 atgcatttga taatagacaa cctatcttaa agctagctta aatcttatgc tgtttgcaca
     5881 ccatcaacat gaggttctgt atatataaaa agtcactata gttattgcaa aaaatgtggt
     5941 aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat gaaagaggaa tagcaaaaga
     6001 aatcctagat gctacagagg gatttttacg tgcatagttg ttctaacaat tgctgatact
     6061 agcgatttag tttatttgtt gtataataat ggggaacagc tgaatactta tataagtatt
     6121 ctggctatag cgtttcgcaa aagatccatt ttgttaatga agtatatcca aaataaaatg
     6181 cccatccaac tgtcagaacg cggatgtgct ggtaattttc ctgctgattc tccataagtt
     6241 gagaaatagt gattttgcat atgcttcatt ggaatggctc atatatttag ataaagaaga
     6301 aaatcgacat ctttaaactt ggatgtcata agaagaggtg aagtttgtga gtaccattaa
     6361 aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg cagaagtggg
     6421 cgaggatatt tttattttag attgtgggct aaaatatcct gaaaatgagt tgttggggat
     6481 cgacgtcgta atccctgatt ttacgtattt agaagaaaac attgatcgtg tagctggtgt
     6541 ctttttaaca catggacatg cagatgcgat cggggcactt ccttacttgt tgtcaaaaat
     6601 ttctgtacca gtattcggga cagagttgac gatcgagtta gcaaaattga atgtcagtcg
     6661 taatgaagca gccaaaggat ttaaggattt ccatgtggta gatgagcata cagaaataga
     6721 ctttggtgaa acagtagtga gctttttccg taccactcat acgataccag attcagtggg
     6781 gatcaatctg aaaacaaaag aaggtagtat cgtttatacg ggagatttta aatttgacca
     6841 aacggctatc ccaatgtatc aaacagatta tgcacgattg gcggaaatcg gcaaagaagg
     6901 agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg tagcttctga
     6961 agcccaaata gcagatgaag tatttgatac gatcaaatat tgggaagggc gtatcatcgt
     7021 cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag cagaaaaagc
     7081 acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga attattcgca cagcgatgcg
     7141 tttagaaaaa cttcaattac caagtgaaga tctgcttgtt acgttgaaag acatgaaaaa
     7201 ctatgatcca gaagaactga tcattttaga aactggccga atgggtgaac ccatcaagtc
     7261 tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag attgaagaag gagatttggt
     7321 ttatatcact actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa cggaagatat
     7381 catttatcga gctggtggaa cagtgaagtt gatctctgag aatatgcgcg tttctggcca
     7441 tgccaatcct aatgacttgc aattaatgat caatttgatg aaaccaacat attttgtgcc
     7501 agttcaagga gagtatcgtg aattagctgc tcatgcagat ttggctcatg cagtagggat
     7561 gccatacaaa aatatctata ttactggacg cggagacgtg ctggaatata agaacaagcg
     7621 tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg attgatggat taggcatagg
     7681 tgatatcgga aacattgttt tacgtgatcg aaaaatcttg tcagaagacg ggattttcgt
     7741 agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc aaattacttc
     7801 acggggattc gtttacgtaa aagctagccg tgatctgatg aaagaaagca gcaatatgat
     7861 tgaaacaatc gttgagaaac atttagaaag caatgatttt gagtggagca aattaaagca
     7921 agacatccga gatcaattag gccgttactt atttgaacaa acaaaacgcc gcccagtgat
     7981 cttgccagtc attatggaag ctactcaacg tcgaaataaa aaataaacaa aaagagtaag
     8041 atgaacctct ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa ataatttttg
     8101 aatacagcat ccctcgcttt ataataaacg atagaataaa gggagtgaat caatgatgag
     8161 tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc agctatcaac cacttgattt
     8221 tagtcatgtg gtagaagaat ctttcacaga agaactacaa aaaagaggga ttgatccagc
     8281 tttgatatgt aaaactctcg tagtgaaagg aaacaaaact ggggtgatca tcgcattgat
     8341 tcctttgaac gatcacttag attacaaaaa aacgagacaa gtgacgaaag atcgtaaagt
     8401 tggttttcct ggaatggatt ttgtattaat gcatacagga tatccccacg gagcaaacac
     8461 ccctattgga ataaaactcg cacatcctga ttattgtttt ttagcagaca gttctttaaa
     8521 agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc ggataaaagt
     8581 agccgattta gaaaagatcg tccatcctat ttacgctgat ttaattagtt aagggacgag
     8641 aagagaagct aagcagagca gagacctcaa acttgagatt atatctgctt ggtacttggt
     8701 gcatcctgta ttatctgtta caattgcttt gtacatactg tactatcaat ccaatgaaaa
     8761 tatatttagc agctatattt tttcattgat gtgcaagtga tgaaatcgtt tttaataaaa
     8821 aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg acattcctgt
     8881 ttaacaattg aaatggaaaa tggccaacgc gtgatgattg acccgtttat tacaggaaat
     8941 ccattgacag acttaactgc agaagaagta aagacagatt ggttgctgat tactcatggt
     9001 catagtgacc acataggaga tatgattcct attgcaaaag aaaatcaagc cacaattatc
     9061 agtattgttg agattgctga ttatgcgaac agcagaggag tagattcatt cggtatgaat
     9121 atcggtggaa aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc acagcatagt
     9181 tcaagctatg aagtagacgg tatcgttcag tatatgggcg agcctagcgg aattattatt
     9241 caagcagaag gaaaaacgat ttatcatgca ggggatactg cttatttcag tgatctagga
     9301 ttgcttgcag aagaattcga tattgatgtc gctttcttgc cgattggcga taactatacg
     9361 atggggccag aagatgcagc aaaggctgcg caaacaattc gtgccaagaa agtagttcca
     9421 atccattaca atactttccc tgtcattgaa caagatcccg aatcttttgt agagttactg
     9481 gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta ggcagaaaat
     9541 ggcgacaaaa catgatttga ttttagaata tattgaaagt ttacctgttg gcaatcgaat
     9601 ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt accgtgcaat
     9661 caaagatgca gaaaatgtcg gactagtttc aacaattcaa cgagtaggga ccatccgtat
     9721 cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg tcaggatcat
     9781 tgaaggggac gttttaggcg gggcggcagg attagacaag gtcttgaata aattcgtcat
     9841 cggcgcgatg actaaacatg ccatggaacg ctatattacg ccaggatcac taatgatcgt
     9901 cggcaaccgg gcggaagtcc aaaaattagc attggaagat ggcgccgcag tattgatcac
     9961 aggaggattc aatacgactg ctgagattgc aaagctagct gatgatcttg aaatgccgat
    10021 cttacgcacc tcttatgata catttacagt cgcaaccatg atcaaccgag cgttaagcga
    10081 ccaattgatc aaaaaagata tcatgcttgt cagtgatatc tatatgccag ctgaaaagac
    10141 tcattatttg cagcagacag atacgatttc tgattatcag cgtttgtcag aagaaacgca
    10201 gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg atggggattg ttacggcaaa
    10261 agatgtttta gggaaatcgc caaaccagat catcgatcgg gtgatgacga aagaaccaat
    10321 cagtgtaaag aaaacaatga gtattgcctc tgttagccat caaatgatct gggatgggct
    10381 agaagtcatg ccggtcgttt ccgatgactt gacattggaa ggcttgatta cccggcaaga
    10441 tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag attgcagata cgatttcgga
    10501 tcagatatct ggaaatatcc atacgattga tactgaccgt gaaggaaatc gtttggaaaa
    10561 ccctaagttc aaattcgtag tagcaccaca aatggtcaat ggcgttggaa ccatttcttt
    10621 tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa acaatggtga tgaaccaaaa
    10681 acgaaatatc ttgattgaac aagtgaacct acattatttg cggctgatcc aattggaaag
    10741 cgaattagac attcgtccac gtgtattgga aatcggcaga cgttccgcta aactggatat
    10801 cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc aagtaatgga
    10861 acgttcttag aaagcaggag tgaaatgaca gtacagcaag aaatagtaga tacaataaaa
    10921 aaatacgata aaatcattat ccacaggcat ttgcgccctg atcctgatgc attaggatca
    10981 caagtaggat tagccgaact attacgggaa agtttcccag aaaaagaaat acgtcaagta
    11041 ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc aagaagttga agatgtcttc
    11101 taccaaggag cgctagtcat cgtgactgat acagctaatt cacccagaat cagtgatcaa
    11161 agatatcagt tggcagaaaa actgatcaaa atcgaccatc atcccaatga tgacccttat
    11221 ggagatcttg tgtgggtaaa tacaaaagca agtagctgta gtgagatgat tgcagaattt
    11281 gctttgatgt ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt actttatgca
    11341 ggaatcgtcg gagatacagg gcgctttttg tatccctcca cgacgtcccg tacattagag
    11401 attgcttcga tattgagaaa ttatccattt gatgcaagtg ctttgaacag agagattgaa
    11461 caaatgccga tgaaagtcgc taaactttcc ggttatcttt atcaaaatat ccaggtggat
    11521 gaaaatggtg caggaagaat aatccttccg caagaaattc ttgataaata tggaatcgat
    11581 gattcagaga cagcttcgat cgtttcactt cctggagtga ttgacgaagt gctggcttgg
    11641 gggatttttg ttcagcagcc ttcagggtat taccgcgtac gtttacgttc taaggggccg
    11701 atcatcaatg aattggctaa acgccatcat ggaggaggcc atccattagc aagtggtgcg
    11761 aatgcaaaag atttagcaga agtagaggaa atctataaag agatccaaga tatatgccaa
    11821 tcgtataaga gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt ttgtagtaaa
    11881 aatcacaaat gtatcttttc aagatcttct ttttccgtta caatagtgct cactaaagat
    11941 tttaaaggag aaagaagatg acattaccaa attgcccaga atgtggctca gagtacacat
    12001 atgaagatcg aggcttgttg atttgcccag aatgcgggaa cgaatggaac aaagaagaac
    12061 aagaaactgt catagaagac ggattagtgg tattagatgc aaatggaaac cgtctgttag
    12121 agggcgatag cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca ggtgccatta
    12181 aacaaggaac aaaagtcaag aacatccgtt tagtagaagg cgatcataat atcgattgca
    12241 aggtagaagg atttggaccg atgaaactga aatcggaatt tgtgaaaaag aattaaagaa
    12301 tattcaaggt cttttcacat tcttttccta ctactaatga taaaataaag taaacgaaaa
    12361 ggagtgaaac gaaaatgtat ggttatggaa tgtatggttt tttagatccg acatttcttt
    12421 tggtcattct tggattagtt atttccggaa ttgcgtcagc gtacgtcaat agtactttcc
    12481 gaaaatacga tcaagtgaga agcaaaaaca atgtgactgg tacacaagca gcacaatata
    12541 tattacaaag tcaacagata aataatgtcg gtgttcaaca aattgccgga gatctcacag
    12601 ataactacaa ttcaggtaat aagatgttga gtttgtctca agcaactgca cagtctactt
    12661 cagttgctgc gataggtgta gcagctcatg aatgcggaca tgcggtacaa gatgcttcgg
    12721 gttatgtccc attgaagatt cgtgcagcac ttgttccagc agctaacttt ggttcgatga
    12781 tttcgttccc cttgattatg gtaggtgtat tgcttagctg gaactctacg ttaatcaata
    12841 tcgggatttt cgctttttct ttagcattac ttttccaatt ggtgacatta ccagtcgaat
    12901 ttaacgcgtc tcggcgagca atccaaattt tgagtgaagg cggactactg acggaagaag
    12961 aagtaccgat ggcaagacac gtattatttg cagcagcact gacttatgtt gcagcagcgc
    13021 tttcgacatt cctgcagctg ttgcgcttga tcttattgtt cggcggcaac cgaagagatt
    13081 aaaataaaga agttgtgaaa tcagcgcttt gatctgagct aataccgaga gttagctgat
    13141 tgaataccat ttatttggaa ta
//
LOCUS       DAZUMO010000077        12819 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000077 DAZUMO010000000
VERSION     DAZUMO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.077"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2155
                     /gene="acm"
                     /locus_tag="ACG8ER_002312"
     CDS             <1..2155
                     /gene="acm"
                     /locus_tag="ACG8ER_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS5050848.1"
                     /translation="RNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNI
                     TDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTV
                     RKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTK
                     PASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPST
                     FEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVS
                     YKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIF
                     KYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEA
                     PNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFK
                     LFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVP
                     SGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEV
                     SEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPG
                     SHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKS
                     ILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2427..2912)
                     /locus_tag="ACG8ER_002313"
     CDS             complement(2427..2912)
                     /locus_tag="ACG8ER_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS5050849.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3002..3347
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3392..4033
                     /locus_tag="ACG8ER_002314"
     CDS             3392..4033
                     /locus_tag="ACG8ER_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5050850.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4052..4687
                     /locus_tag="ACG8ER_002315"
     CDS             4052..4687
                     /locus_tag="ACG8ER_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5050851.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4705..5520
                     /locus_tag="ACG8ER_002316"
     CDS             4705..5520
                     /locus_tag="ACG8ER_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5050852.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5839..7275
                     /locus_tag="ACG8ER_002317"
     CDS             5839..7275
                     /locus_tag="ACG8ER_002317"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5050853.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7464..9911
                     /locus_tag="ACG8ER_002318"
     CDS             7464..9911
                     /locus_tag="ACG8ER_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5050854.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10141..11208
                     /gene="rlmN"
                     /locus_tag="ACG8ER_002319"
     CDS             10141..11208
                     /gene="rlmN"
                     /locus_tag="ACG8ER_002319"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5050855.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11198..11404
                     /locus_tag="ACG8ER_002320"
     CDS             11198..11404
                     /locus_tag="ACG8ER_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050856.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11401..12504)
                     /locus_tag="ACG8ER_002321"
     CDS             complement(11401..12504)
                     /locus_tag="ACG8ER_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5050857.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4373 a         2160 c         2714 g         3572 t
ORIGIN      
        1 tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt
       61 tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga
      121 cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa
      181 acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg
      241 cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt
      301 aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc
      361 agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga
      421 aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa
      481 gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga
      541 agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca
      601 accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga
      661 aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca
      721 atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata attacattga
      781 aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat
      841 catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt
      901 taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc
      961 tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac
     1021 agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca
     1081 aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct
     1141 gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc
     1201 actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt
     1261 acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg
     1321 aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga
     1381 accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga
     1441 agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga
     1501 tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt
     1561 tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga
     1621 gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc
     1681 gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc
     1741 aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata
     1801 cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat
     1861 atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga
     1921 gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag
     1981 cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc
     2041 aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt
     2101 attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga
     2161 atataatatt ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttataatc
     2221 gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg
     2281 gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt
     2341 tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat
     2401 tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag
     2461 taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt
     2521 ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt
     2581 ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg
     2641 tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt
     2701 tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt
     2761 cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg
     2821 actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt
     2881 tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt
     2941 gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa
     3001 acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg
     3061 gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt
     3121 aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg
     3181 cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt
     3241 cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc
     3301 aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga
     3361 ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca
     3421 attattagta ggagcactca caacattgaa attattttta tttaccttga ttggctcatt
     3481 accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt
     3541 gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat
     3601 tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc
     3661 ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat
     3721 tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac
     3781 gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat
     3841 caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc
     3901 tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat
     3961 ctatcttaca ttgactggta ttctaacaat cataatgaaa caagttgaag gaaaattaaa
     4021 ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc
     4081 atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt
     4141 agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt
     4201 agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct
     4261 taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct
     4321 gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga
     4381 atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat
     4441 ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc
     4501 tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg
     4561 ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt
     4621 cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat
     4681 tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca
     4741 ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact
     4801 atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt
     4861 agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa
     4921 caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg
     4981 aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag
     5041 aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa
     5101 tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca
     5161 tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa
     5221 gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc
     5281 gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta
     5341 gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc
     5401 aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca
     5461 ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa
     5521 caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc
     5581 tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg
     5641 tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     5701 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata
     5761 attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta
     5821 taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg
     5881 tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga
     5941 gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt
     6001 gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa
     6061 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
     6121 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
     6181 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
     6241 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
     6301 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
     6361 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
     6421 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
     6481 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
     6541 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
     6601 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
     6661 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
     6721 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
     6781 ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct
     6841 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
     6901 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
     6961 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
     7021 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     7081 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     7141 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     7201 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     7261 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     7321 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa
     7381 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
     7441 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
     7501 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
     7561 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
     7621 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
     7681 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
     7741 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
     7801 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
     7861 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
     7921 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
     7981 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
     8041 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
     8101 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
     8161 ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag
     8221 cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc
     8281 cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc
     8341 tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg
     8401 agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc
     8461 aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg
     8521 atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg
     8581 gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc
     8641 agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg
     8701 ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta
     8761 ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac
     8821 caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac
     8881 aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga
     8941 agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag
     9001 taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc
     9061 atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag
     9121 tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg
     9181 gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac
     9241 cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag
     9301 ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc
     9361 tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt
     9421 ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg
     9481 gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac
     9541 cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg
     9601 ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa
     9661 cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg
     9721 ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc
     9781 gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa
     9841 gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata
     9901 atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag
     9961 gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt
    10021 cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca
    10081 ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact
    10141 ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa
    10201 aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta
    10261 agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat
    10321 tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa
    10381 tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag acaagaatat
    10441 ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca
    10501 agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg
    10561 atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta
    10621 atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc
    10681 aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta
    10741 gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg
    10801 cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt
    10861 gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt
    10921 gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat
    10981 ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt
    11041 gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg
    11101 aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct
    11161 tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact
    11221 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
    11281 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
    11341 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
    11401 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
    11461 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
    11521 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
    11581 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
    11641 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
    11701 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
    11761 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
    11821 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
    11881 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
    11941 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
    12001 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
    12061 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
    12121 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
    12181 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
    12241 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
    12301 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
    12361 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
    12421 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
    12481 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
    12541 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
    12601 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
    12661 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc
    12721 taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata
    12781 tgaaaacagg ttttatttgt caattcataa ttggttttt
//
LOCUS       DAZUMO010000078        12285 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000078 DAZUMO010000000
VERSION     DAZUMO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.078"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..648)
                     /gene="frr"
                     /locus_tag="ACG8ER_002322"
     CDS             complement(91..648)
                     /gene="frr"
                     /locus_tag="ACG8ER_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS5050858.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(651..1373)
                     /gene="pyrH"
                     /locus_tag="ACG8ER_002323"
     CDS             complement(651..1373)
                     /gene="pyrH"
                     /locus_tag="ACG8ER_002323"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS5050859.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1509..2390)
                     /gene="tsf"
                     /locus_tag="ACG8ER_002324"
     CDS             complement(1509..2390)
                     /gene="tsf"
                     /locus_tag="ACG8ER_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS5050860.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2489..3271)
                     /gene="rpsB"
                     /locus_tag="ACG8ER_002325"
     CDS             complement(2489..3271)
                     /gene="rpsB"
                     /locus_tag="ACG8ER_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS5050861.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3630..4109
                     /locus_tag="ACG8ER_002326"
     CDS             3630..4109
                     /locus_tag="ACG8ER_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5050862.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4326..5417
                     /gene="phnW"
                     /locus_tag="ACG8ER_002327"
     CDS             4326..5417
                     /gene="phnW"
                     /locus_tag="ACG8ER_002327"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS5050863.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5410..6195
                     /gene="phnX"
                     /locus_tag="ACG8ER_002328"
     CDS             5410..6195
                     /gene="phnX"
                     /locus_tag="ACG8ER_002328"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS5050864.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6541..8232)
                     /gene="argS"
                     /locus_tag="ACG8ER_002329"
     CDS             complement(6541..8232)
                     /gene="argS"
                     /locus_tag="ACG8ER_002329"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS5050865.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG8ER_002330"
     CDS             complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG8ER_002330"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS5050866.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG8ER_002331"
     CDS             complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG8ER_002331"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS5050867.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG8ER_002332"
     CDS             complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG8ER_002332"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS5050868.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3385 a         2542 c         2195 g         4163 t
ORIGIN      
        1 aattttttct ttttttaatt tttaaataaa agaaatataa ataaaaacag aggaagcgga
       61 atgatccgcc cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc
      121 agcagcgatc ttatctactt ctttaatgct gttatctgtc aatgtttgta cgtctttttc
      181 taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc
      241 gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac
      301 atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac
      361 atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc
      421 ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat
      481 tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa
      541 aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc
      601 tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc
      661 cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt
      721 aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc
      781 atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca
      841 tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg
      901 ataacatctg cgccgatttc tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac
      961 gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct
     1021 ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc
     1081 ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac
     1141 atacccatgt aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc
     1201 cagatatttc cgccacccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg
     1261 atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc atccccagct
     1321 aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt
     1381 cctccaatat tttctattgc cattttacca tacttagatg atttcgacac tctaagactg
     1441 gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt
     1501 caacagaatt attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt
     1561 tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca
     1621 acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt
     1681 tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg
     1741 ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga
     1801 atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt
     1861 gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata
     1921 tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag
     1981 ctgatttttt ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta
     2041 tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct
     2101 gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag
     2161 tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct
     2221 gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact
     2281 gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg
     2341 ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc
     2401 tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca
     2461 gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa
     2521 catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta
     2581 cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt
     2641 taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc
     2701 agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa
     2761 tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga
     2821 taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt
     2881 taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga
     2941 tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga
     3001 agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac
     3061 ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat
     3121 ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga
     3181 tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt
     3241 ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt
     3301 tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat
     3361 aagaatgtgg atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg
     3421 tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa
     3481 agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat
     3541 aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca
     3601 tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta
     3661 aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc
     3721 attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg
     3781 tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc
     3841 ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga
     3901 aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat
     3961 ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg
     4021 atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc
     4081 accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt
     4141 gtttttttat tttcaatatt ttccttatta aataaacctt tacactcttt acaaaaatac
     4201 atcatttttt attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat
     4261 tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa
     4321 atcatatgta taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg
     4381 ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa
     4441 gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc
     4501 aaggtagcgg ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata
     4561 ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc
     4621 tgaaactgaa ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga
     4681 ttacacagct gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga
     4741 ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc
     4801 ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac
     4861 tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt
     4921 cttttgtact tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt
     4981 cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca
     5041 cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag
     5101 gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa
     5161 tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt
     5221 ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa
     5281 acggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata
     5341 ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg
     5401 agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc
     5461 tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact
     5521 gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt
     5581 tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa
     5641 gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat
     5701 tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat
     5761 attggttcca cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa
     5821 aaagaagggt attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga
     5881 ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa
     5941 gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg
     6001 acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa
     6061 ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct
     6121 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6181 aagaatccag aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa
     6241 gtaataagaa cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6301 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac
     6361 ttttgtcaca gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa
     6421 acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct
     6481 attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc
     6541 ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt
     6601 cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt
     6661 atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga
     6721 cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac
     6781 cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc
     6841 tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc
     6901 tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt
     6961 caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc
     7021 caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac
     7081 ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat
     7141 caaaccaaat ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac
     7201 agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt
     7261 gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt
     7321 cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat
     7381 ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc
     7441 catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc
     7501 atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc
     7561 ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg
     7621 ttctgtttca gctacttcat ggaattggac atataaacga agcaattcat tgattggttc
     7681 tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg
     7741 tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat
     7801 gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc
     7861 aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc
     7921 ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt
     7981 catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat
     8041 tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga
     8101 aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc
     8161 gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc
     8221 tttattattc atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct
     8281 ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc
     8341 aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac
     8401 agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt
     8461 cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt
     8521 tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc
     8581 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8641 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8701 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8761 tagctgcctc tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt
     8821 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8881 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8941 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     9001 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9061 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9121 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9181 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9241 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9301 ccttcagtac ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg
     9361 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9421 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9481 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9541 ttgcatccgt agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca
     9601 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9661 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9721 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9781 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9841 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9901 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9961 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    10021 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10081 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10141 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10201 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10261 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10321 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10381 gttcctctac cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta
    10441 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10501 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10561 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10621 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10681 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10741 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10801 agtaagaccg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10861 tgcagcgtgg accaccacgg cctcgtgaca attcacttga attgatttct aacactttga
    10921 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10981 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    11041 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11101 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11161 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11221 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg
    11281 ctaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11341 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11401 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11461 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11521 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11581 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11641 cgtatagact gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat
    11701 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11761 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11821 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11881 atgtctttgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11941 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca
    12001 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12061 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12121 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12181 acgtttttct atttttaagc gtaatttatt cattttttat tctgtataca ctttcaaaaa
    12241 aatatttatt cattgaaaaa aattttttga ataaattaaa aaaat
//
LOCUS       DAZUMO010000079        12255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000079 DAZUMO010000000
VERSION     DAZUMO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.079"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..729
                     /locus_tag="ACG8ER_002333"
                     /pseudo
     CDS             118..729
                     /locus_tag="ACG8ER_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            842..1120
                     /locus_tag="ACG8ER_002334"
     CDS             842..1120
                     /locus_tag="ACG8ER_002334"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5050869.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1238..1474
                     /gene="secG"
                     /locus_tag="ACG8ER_002335"
     CDS             1238..1474
                     /gene="secG"
                     /locus_tag="ACG8ER_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS5050870.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1577..2332
                     /locus_tag="ACG8ER_002336"
     CDS             1577..2332
                     /locus_tag="ACG8ER_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5050871.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2335..4692
                     /gene="rnr"
                     /locus_tag="ACG8ER_002337"
     CDS             2335..4692
                     /gene="rnr"
                     /locus_tag="ACG8ER_002337"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS5050872.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4714..5178
                     /gene="smpB"
                     /locus_tag="ACG8ER_002338"
     CDS             4714..5178
                     /gene="smpB"
                     /locus_tag="ACG8ER_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS5050873.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5480..6199
                     /gene="atpB"
                     /locus_tag="ACG8ER_002339"
     CDS             5480..6199
                     /gene="atpB"
                     /locus_tag="ACG8ER_002339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS5050874.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6252..6467
                     /gene="atpE"
                     /locus_tag="ACG8ER_002340"
     CDS             6252..6467
                     /gene="atpE"
                     /locus_tag="ACG8ER_002340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS5050875.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6558..7082
                     /gene="atpF"
                     /locus_tag="ACG8ER_002341"
     CDS             6558..7082
                     /gene="atpF"
                     /locus_tag="ACG8ER_002341"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS5050876.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            7069..7611
                     /locus_tag="ACG8ER_002342"
     CDS             7069..7611
                     /locus_tag="ACG8ER_002342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS5050877.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7634..9190
                     /gene="atpA"
                     /locus_tag="ACG8ER_002343"
     CDS             7634..9190
                     /gene="atpA"
                     /locus_tag="ACG8ER_002343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS5050878.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9204..10106
                     /locus_tag="ACG8ER_002344"
     CDS             9204..10106
                     /locus_tag="ACG8ER_002344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS5050879.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10129..11535
                     /gene="atpD"
                     /locus_tag="ACG8ER_002345"
     CDS             10129..11535
                     /gene="atpD"
                     /locus_tag="ACG8ER_002345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS5050880.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11549..11971
                     /locus_tag="ACG8ER_002346"
     CDS             11549..11971
                     /locus_tag="ACG8ER_002346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS5050881.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4070 a         2187 c         2701 g         3297 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctggt
      121 aaaagatatg cagagaaagg attcaacgtt cttgtacctg atttacgtgc gcacggagaa
      181 agtgaaggag aaatcatcgg aatgggatgg ctagatcggc tagatttaat tgcctggatc
      241 caactcatct tagatgaaca gccgaatgcg tccattattt tacacggcgg atcaatggga
      301 gcttctacga tcatgatggc aagtggtgaa aaacttccat cagcggtcaa aggatttatt
      361 ttagacagcg gatatgtttc tgtttatgca gaattccgat atatgttaag taaaatcact
      421 gtctttccta aaaaaatggt catgagatat gcgaatcatt atgcccagaa atacgccggt
      481 tactccttaa agcaagcatc agctacgaga cagcttggca gcaaccatct gcctttatta
      541 gtcatccatg gtgaaagaga tcattttgtc cctacagaag cagcgtacac gattcaaaat
      601 gctacggcag gggataaagc actgctgctt gttccagaag cagaacactt ggaagcatcg
      661 atgaaagatc cgaaaaccta ttggacagtg attttttcat ttattgaaca aagaataacg
      721 atggattaat tttctcttct tttttgtaag taattcgagg ataaataatt gtttatagat
      781 ctggagactg ctaaaataag gtaggaataa aaaaatatca gttatttgag gagtgcggca
      841 tatggaaatc aaagaagaaa aaaatcgtct tgctttattc aatgatgagc aacaagagat
      901 tggagaaatg acatggtcag atgcgggccc agacatcatg atcattgacc atacatttgt
      961 agatccagca tatcgtggtc aaaagttagc tgaaaagcta gtgtatactg gagtagaatt
     1021 agcaagacga gacgggaaaa agatcattcc tttatgccct tatgcaaaaa aagagtttga
     1081 aaagaaacca gaatatcaag acgtcttacg tcagtcataa gaataacagc tgaaagtttt
     1141 gaactttcag ctgttattcc atttatatag agttgtcacg gacttttatc tatgataaag
     1201 ttaaagagac gatatctttt gggaaggagg ctgcgatatg tataatttaa ttttgggaat
     1261 cgtgatcgtt ttatcgattg ttatggtcat tgcgattatg atgcagccaa gcaagcaaaa
     1321 tagtgcagcg agtgcattta ccggcggtgc ggatcaacta ttcggcaaac aaaaagctcg
     1381 cggctttgaa gctgtcatgc agcgatcaac tgcagttatg ggagctatct ggatgatctt
     1441 attatttgtc ctagcatatt tatcttcgaa gtagttgaat aagcctctcg caaaacggcg
     1501 ggcggctttt tttattgcaa agagaacgct taagaagaag ttatgaaaga aactatggta
     1561 aaatgaggcg aaagaggtga gacgagtgag acaattacca aaaccccttt atgcaaaaca
     1621 tggaaagcga gcggttttgc tgcttcacgc ttactcagga agtccaaacg atgttcgaat
     1681 gctcgcacga tttttggaaa aagcagatta tacagtttat gcgccgcttt ttaaaggaca
     1741 cggaacaatg gagccatatg atatcttgca agaaaaagca gaaagctggt gggcagacac
     1801 taaaaaagcc atccattttt tacagtctga gcagttttct gatattgccg tattaggatt
     1861 atcaatgggc ggcatctttg ctgtacgagc actagaagaa gaatcagtga ttggcggcgg
     1921 ttttttctgt tcgcctttgt ctcctgtaaa aacaaacgtg cctgaaaact ttgaaaaata
     1981 cgtacgtcaa gtgctgaaga cagcaggtaa atcagagaaa gaaatcaatg aaaaagcagt
     2041 tgcttaccgc tcgttagctg aacaacaatt aatggatata caagatcagg cagcaatcgt
     2101 agaaagtagg ttgtcagata tccaacagcc gatattttta gctcaagctg ggaaagatga
     2161 aatgatcgac cctaatggcg tatttgaaac tgcacgcaag ttgagccgtc agcgagtgac
     2221 acttcaatgg tatcctgaaa gtggtcatgt gattacagtt ggtacagcaa gacgcgaatt
     2281 agaaaaagac gtcttggaat ttttagaaaa attaccttgg aatgaggaat aaatatgaca
     2341 aaagaaacga taaaagagaa aattctcttt ttcatggaaa gcagtcgcaa acgcagtttt
     2401 tctatggaag aaatcgctga aggtttaggg ttccaaaaaa gcgatgattt taaattacta
     2461 gtacaaacga ttgctcaaat ggaaagagaa cagagtatag ttttcaataa aaaaggaaaa
     2521 gtgaaacttc ctttaaaacc aatcttgatc gaaggaacat ttcgagcaaa tgaacgagga
     2581 tttggatttg taacgattga tcccgaagaa gatgacatct atatcccaaa agaagcgact
     2641 ggttttgcaa tggatggaga caccgtagca atcgatatca tgaagcgagc aaatacagat
     2701 atggatcgcg gagcagaggg gaaagtagtc gagattcgag accgggcgac tactcagtta
     2761 gcaggggaat tcgttgctta ttcagaagaa gaaatacaag aaacagatct ttacggcgtt
     2821 gttatcccga aagatagaaa gctcaaccaa tttaaagtat atgcagcagc agagggagtt
     2881 cgtccagttg acggaagtat tgtgctgatc gaactgaccc attaccctga aaaagggtat
     2941 gcgactagtt tagaagggat aatcaagcaa gtgattggtc ataagaatga ccctggcatg
     3001 gatatcctct caattgtcgt ggctaatggt atccctacga aatttcctga tgatgtattg
     3061 tctgaagcag atgaagttcc agatcaaatc agcgaaaagg atataactgg acgtagagat
     3121 ttaagaaatc agctcattgt tacgattgat ggagaagatg caaaagatct agatgatgca
     3181 gttacggtta aaaaattaga taacggtaat tattttcttg gagtacacat tgcagatgtt
     3241 tcttactatg tgacacaagg cagtcaattg gacagagaag catatgaaag agggacaagt
     3301 gtatatttga ctgatcgagt gattccgatg atcccgcaac gtttatcgaa tggtatctgt
     3361 tcactgaatc cccatgttcc aaggctgacc atgagttgcg aaatggagat tgatccaaat
     3421 ggacaagtag tctctcatga tattttccca agtgtgatcc aaacaacaga acggatgacc
     3481 tacacagctg tcaatcaaat cttagaagat caagacgaag aaacgatgca acgctatgct
     3541 cagctcgtac ctatgttcca agaaatgggt gaattgcatc gaatccttga aacgatgcga
     3601 atcaaacgtg gcgcgatttc ttttgaggat cgcgaagcga aaatccttgt tgatgctgaa
     3661 ggacacccaa atgacatcca gctaagaagc agaggaattg gtgaacgctt gatcgaatct
     3721 ttcatgcttg cggccaatga aacggtagct gaacattata ataaattaaa tctgccgttt
     3781 atttaccgaa tccatgaaca gccaaaagaa gaaaaaatgc agcgcttctt tgattttgct
     3841 tcagctttgg gcattctggt ccgaggaacg aaaggaacga tcacaccaaa agatttacag
     3901 catgtgatcg aaggagtaga agacaaacca gaagctgcag ttatcaatac tatgttgcta
     3961 agaagcatgc aacaagcacg ctattcagaa gacaattttg ggcattatgg cttagcggca
     4021 gattactaca cacactttac atctcctatt cgacgctatc ccgatctaat cgtccatcgt
     4081 ctgatccgtt cttacagtga agataaaagt gaaagcaatc aaagttcgtg ggctgcggaa
     4141 cttcctgaaa ttgcagatca tagttctaag atggaacgtc gtgcagtaga aacagaacgt
     4201 gaagtagatt ccatgaagaa agcagaattc atgatggaca aagtgggaga agaattcgat
     4261 gggatcatca gctctgttgt gaaattcggc ctgtttatcg aactgccgaa tacagtagaa
     4321 ggattgatcc atatcaatga attgaaacag gactacttcc attttgttga aaatcattta
     4381 gctcttgtag gtgaaagaac tggtctgact tttaaaatcg gacaaaaagt gcgcgtaaaa
     4441 gtagtcaaag ctgatccaga tgaacgagca gtggattttg aactcgtttc cgcagaagag
     4501 gtcactgcgt tggaacgtcc aaaagcacgc aatggaaaat ctggaaaacg agaaggaaaa
     4561 caagcaggca aacgctcaga aaaaaatggc aagaagaaca aacgatatag tgatagtaag
     4621 caaacgttcg gtaagaaaaa agggaaaaag ccattttaca aagatgtcaa aaagaagaaa
     4681 aagaaaaaat aaaatgagtt ggaggaaaca gctatgccaa aaggcgaggg aaaactaatt
     4741 gcacaaaaca agaaagctcg ccatgattat tcgatcatcg acacgatgga agcagggatg
     4801 gtcttgcaag gaacggagat caagtcgata cgaaacagcc ggatcaatct gaaagatgga
     4861 tttattcgcg tccgcaacgg ggaagctttc ttgcataatg ttcatatcag tccttatgaa
     4921 caaggaaata tttttaatca tgatccgttg cgcacgagaa agttattatt acacaagaaa
     4981 caaatcgttc gtcttgaaaa cgaattgaaa aacactggaa tcactgttgt tcctttaaaa
     5041 gtctatattc gtaacggcta tgccaaggta ttgattggtc tggcaaaagg gaaaaaatcg
     5101 tatgataaac gggaagattt gaaacgaaaa gatatcgatc gacaaattga tcgaacatta
     5161 aaaaatttct ctagataatt tcattatcct ttttcgcata ttcggtgctt ttgcttttac
     5221 ttaatgataa aatttgttag catttaatct gataaaacat gattatcaca ggttttttct
     5281 tagatcagga gcaccgtgca tggggaaaaa ttcgcttcgt ttttgactga aataaaaaag
     5341 tgtctaattt tcatgaaaat gttttgaatt ttctgtttca tggtggtatg ataccgtgtg
     5401 gttagaaacc gattggaaaa aaccagatac gcacatgaat tgattttatg tgaatttcgg
     5461 ggaaaagagg tgaaatggat tggatgaacg ctcgctaacg tttcacattg gaccaatttg
     5521 gtttgatggt acagtttgta taatggtgtt actgacctgt ttgattgttt tcttcttagc
     5581 ttactttttt acaagaaatc ttaagttgaa accaacaggc aaacaaaatg cgatagaatg
     5641 ggtcattgat tttagtcgag gaatcgtaac agataatctg cctagaaaag aattgagcaa
     5701 tttccatttg ttggcattta cattgttttt atttgttttt gttgcaaaca acataggttt
     5761 agtaacaaaa atcgtcttac ctaacgaaac aacactttgg aaaagcccga cagcagaccc
     5821 gtttgttact ttaactttgg catttataat gatcttactt actcacttat tcggggtgaa
     5881 aaagttaggc ttcaaaggat atttcgtaaa ttcgttcttg aaaccctata gttttatgtt
     5941 tccgatgaaa ctgattgaag aatttacaaa tttattaaca cttgctttac gtctttatgg
     6001 gaatatttat gcgggggaag ttttgctaac cttgattgca aaaatgatga caagcttagg
     6061 ctggttctca ttaccattag caattccatt agagatggtc tggatcgcgt tctccttgtt
     6121 tattgggagc atccaagcat ttgtctttgt cactttgtca atggtttata tgagccataa
     6181 gattgaagta gaagaataat actcggttaa aaaacaaact actatttatc tattttagga
     6241 ggaaaacaat tatgaattat atcgcagcag caatcgcaat catgggagca gcaatcggag
     6301 ccggttatgg taacggtcaa gttatctcta aaacaatcga atcaatggct cgccaacctg
     6361 aaatgtctgg tcaattccgt acaacaatgt ttatcggggt agctctagtt gaagctgttc
     6421 ctatcttagg ggtagttatc gccttgattc tagtttttgc cgtttaattg aatcagtatt
     6481 aaggcagaaa caacatcgtt tgttactgct tttttacatg gaaactagaa atactccaaa
     6541 gaaaggaagg ctcgcctatg ctgaatcaat tggcaattgc agaagttggc aatgcgatgt
     6601 taggtaatat catcgttgtc agcggctcat ttttgatact actggctctc ttgaagcact
     6661 ttgcttgggg accaatcagc gatattttga aaaaacgtga agacaagatc gccaatgatt
     6721 tagattctgc agaacaatct cgcatcaact cagcgaaaat ggaacaagaa cgcgaacaac
     6781 aattgttagc ctctcgttct gatgcagctg atatcatcaa aaatgcgaaa gaaagtggag
     6841 aattaagccg ccaaaatatt ttgaaggatg ctcaagaaga agcagctcgt ctaaaaagca
     6901 aagcccaagc tgatatcact gtagaacgtg attcagcgct gaactctgta aaagacgacg
     6961 ttgcagaact ctctcttcaa atcgcggaaa aaatcttgaa caaagaatta tctccagaaa
     7021 tgcatgaatc attaatcaat caatacattg aaggtctagg ttcttcaaat gaaactagat
     7081 aaatatacag taggaagacg ttacgggaaa gctttatttg aactggcgat cgattcaaat
     7141 caagcagaag aaatctatca agatctcgta agtttacgtc aaatctatga acaagtacct
     7201 ggattaggga atatgttaag tgatgttcgt ctagaacctc atgaaaaacg agaaatcatg
     7261 gacaagcttg tcagcggtta tgatgggatc gttaaaaact ttttagaagt tgtttatgag
     7321 tataaccgaa tggatgacct tttgttcatg atcgaagaat atgaacgtcg atatgatgaa
     7381 catcaagggt tactattggg aagtgtcaca acagcagtcc cactttcaga cgaacaacga
     7441 atacaattag aaaagaatgt tgcaaaaaca atggactatc aatcagttga attaaaacaa
     7501 atcgttgatt cttctattat tggtggcgcg attgttgaag ccaaccatcg agtaatcgac
     7561 ggaagcattc gcacgcaatt agaaaaaatg cgtaatcaat taaacagata gtacaaagag
     7621 gtaggtgaat cgaatggcca tcaaagcaga agaaattagt gccttgatta aagaacaaat
     7681 tgaaaattat caaaacgtgc tttcagtcga agaaatcggt accgtaactt atgtaggtga
     7741 cgggatcgct cgtgcacatg gtttagaaaa cgcgatgagt ggtgagttgc ttgaattttc
     7801 aaacggctca tacggaatgg cgcaaaactt agagtcaaat gatgtaggta tcattatcct
     7861 tggcgatttc gagtccattc gtgaaggaga taaagtaaaa cgaacaggta agatcatgga
     7921 agttccagtt ggagaggcct tgatcggtcg ggtagtcaat ccgctaggtc aaccaatcga
     7981 cggactaggt gaaatcgtta ctgacaaagc tcgtccagta gaagcgatgg caccaggtgt
     8041 tatgcaacgt aaatctgtta acgaaccaat gcaaacaggg ctaaaagcga tcgacgccct
     8101 tgtgccaatc ggacgcggac aacgtgaatt agtcatcggt gaccgtaaaa cagggaaaac
     8161 ttctattgcg atcgatacga tcatcaacca aaaaggtcaa gatatgatct gtatctatgt
     8221 agcaatcgga caaaaagatt ctacagttcg tacacaagtt gaaacattga aaaaatatgg
     8281 cgcaatggat tacacaatcg ttgtgaatgc cggtgcgtct caaccagcac cattgcttta
     8341 tatcgcacca tatgctggta ctgcaatggg tgaagaattc atgtacaacg gtaaacatgt
     8401 attgatcatt tttgatgatc tttcaaaaca agctgttgct tatcgtgaac tttccttgct
     8461 acttcgccgt ccaccaggtc gtgaagctta tcctggggat gttttctact tgcactcacg
     8521 tttattagaa cgtgcagcaa aattaagtga tgatttaggt ggcggttcaa tgactgcctt
     8581 accatttgtt gaaacacaag ctggagatat ctctgcttat atcccaacaa acgttatttc
     8641 gatcactgat ggacaaatct tcttagaaag cgacttgttc tacgcaggta ctcgtccagc
     8701 ggttgatgcc ggactatctg tatctcgtgt agggggttct gcgcaaatca aagcaatgaa
     8761 aaaagttgct gggactttac gcctagactt agcaagttac cgtgaattag aagcatttac
     8821 acaattcggt tctgacttgg atgcagcaac acaagcaaaa ctaaatcgtg gacgccgtac
     8881 agtagaaatc ttgaaacaaa aattgcatgc accgctagct gttgaaaaac aagtagtgat
     8941 tttgtatgct ttgacacatg gattcttaga tagtatccct gtagacagta ttttagactt
     9001 cgaacatgaa ttattcgaat atctggatac caatcattca gatatctttg aaacaatccg
     9061 tacaacaaaa gatcttccag aagaagaagc gttgaacagt gcgattcagg aatataaaga
     9121 catgttctta gctacaaaag gaagtaattc ctctacagaa gataaattga aatccattca
     9181 aaatgcatag tgaggtgaga tgaatgggtg cttcattaaa tgaaatcaag acacgtatcg
     9241 cttcaacgaa aaaaacgagc caaatcacgc gggcaatgca gatggtgtct gcttctaagt
     9301 tgacaaaatc tgaagcttct tcacagaagt ttcaggttta tgcaacaaaa gtaagagaaa
     9361 tcgttacgca tttgactgct actcagttaa atgatattgc ctcagaaaat cctagagggg
     9421 atatcaatta caacagcatg ctgctcagcc gtcctgtgaa gaaaactggt tatatcgtca
     9481 tcactgcaga tggcggttta gtcggtggat ataacagctc gatattgaaa caaacgatgt
     9541 ctattttaga agaagaccac caatcacccg atgattacgt gatgatcgca attggcggaa
     9601 ctggtgcaga cttctttaaa gcacgcggta tcaatctagc atatgagctg cgcaatctat
     9661 ctgatcagcc aagctttgat gaagtccgca aaatcgtaaa tatggcaaca actatgttcc
     9721 aaaacgaagt attcgatgaa ttatacgtat gttataacca tcatatcaat tcgctaacta
     9781 gtcagtttcg tgtagaaaaa atgctgccaa tctcagactt agatccagaa gaagcaacga
     9841 ctttcgaaca agaatatatt tttgaacctt caaaagaaga aatcttgtct cagttattgc
     9901 ctcagtatgc tgaaagtttg atctacggtg cgatcgttga cgccaaaaca gcagaacatg
     9961 ctgctggtat gacagcaatg aaaacagcaa ctgataatgc tgctaatatc attgatgagc
    10021 tgacggtttc ttataaccgt gcaagacaag gggctattac ccaagaaatt accgaaattg
    10081 ttgccggagc ttcggcacta gaatagtatt gagattggag gaaaaaagat gagttcaggc
    10141 aagattgttc aagtaatcgg tcccgttgtc gacgtggaat tttctttaga ccaatcctta
    10201 ccagatatca acaacgcatt agttgtttat aaaaatgacg aaaataaatc aaaagttgtt
    10261 cttgaagcag cgttagaatt aggggacgga gtgatccgga ccattgcgat ggaatcaaca
    10321 gatggtttac aaagaggaat ggaagtcatt gatacaggca aagcaatctc tgttcctgta
    10381 ggtaaagaaa cattaggtcg cgtattcaac gtactaggag atacgatcga tttagaaaca
    10441 cctttcccag aagatgcgga aagaagcgaa attcataaaa aagcaccagc ctttgacgaa
    10501 ttaagtacaa gtacagaaat tttggaaaca gggatcaaag ttatcgattt gcttgcccca
    10561 tatttaaaag gtgggaaagt cggactattc ggtggtgccg gtgttggtaa aaccgtactg
    10621 atccaagaac tgatccataa tatcgcccaa gaacatggtg gtatttctgt atttaccggt
    10681 gtaggtgaac gtactcgtga aggtaatgac ttgtattatg aaatgaaaga ttcaggagtt
    10741 atcgaaaaaa cagccatggt gttcggacaa atgaacgaac caccaggtgc acgtatgcgt
    10801 gttgctttga ctggtttgac gattgcggaa tatttccgtg atgtagaagg tcaagatgta
    10861 ctgttgttta tcgacaacat tttccgtttc actcaagctg gatctgaagt atcagccttg
    10921 ttaggacgga tgccttctgc ggttggttat caaccaacat tggcaacaga aatgggtcaa
    10981 ttacaagaac gtatcacatc tacgaaaaaa ggttcgatca catcgatcca agcaatctat
    11041 gtgccagccg atgactacac tgacccggcg ccggcaacag cattcgccca tttggatgcg
    11101 acaaccaact tggaacgtaa attaacagaa caagggattt atccagcggt agatccattg
    11161 gcttcttctt caagtgcttt agcacctgaa atcgtggggg aagaacatta taaagtagca
    11221 acagaagtcc aacacgtatt gcaacgatat cgtgaattac aagatatcat tgctatctta
    11281 ggtatggatg aattatccga tcaagaaaaa gtgttagttt ctcgtgcccg cagagtccaa
    11341 ttcttcttgt cacaaaactt taacgtggct gaacaattta ctggtcttcc aggttcatat
    11401 gtccctgtag aagaaacagt gaaaggattc cgcgaaatct tggaaggcaa atacgatgat
    11461 cttcctgaag aagctttccg aagtgttggc cgaatcgaag acgtcgtaga aaaagcgaaa
    11521 acactaggct actagaaagg ggtgccccat ggatcaatat ttaacagtaa atgtggtaac
    11581 tcctgccggt ttggtctatg atcaccatgc agctatcgtc gttgcacgta cgacagctgg
    11641 tgagcttggg attttgccta aacatgcacc aatcatcgtt ccattgacga ttgatgaagt
    11701 tcgtgtaaaa cgaacagatt cagataccca cgtcgattgg atcgctgtta atggcggaat
    11761 catggaagtt cgtgataatg ttgtttcaat cgttgctgat agtgcggaac gtgaacgtga
    11821 tatcgatgta tctcgtgcgg aacgtgcaaa acaacgcgca gaacgccaaa tcgaagaagc
    11881 aaaagcaaaa caagatacga acgaattgaa acgggcaact gttgcgttgc accgagcaat
    11941 caatcgtatc aaagtatcta agcactcata aaatgagatg cggatgttta tccgtgttta
    12001 agaacagtaa aaagaggtta tgacagaagt ggtcagcttc aagacataag aaggaattta
    12061 cgaaaattgc ttctcaaatt tttgtgaaat ctcagcttat ttttcgaaga agctacttct
    12121 ggaacaccgt atatcagagg ttgtaaaatc agcgttttgt cctgagtatt agagaaacaa
    12181 atggaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagatgatt
    12241 gaacaccgtt tattt
//
LOCUS       DAZUMO010000080        12136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000080 DAZUMO010000000
VERSION     DAZUMO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.080"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..960
                     /locus_tag="ACG8ER_002347"
     CDS             181..960
                     /locus_tag="ACG8ER_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050882.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTIVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIDQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            1006..1950
                     /locus_tag="ACG8ER_002348"
     CDS             1006..1950
                     /locus_tag="ACG8ER_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5050883.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1966..2745
                     /locus_tag="ACG8ER_002349"
     CDS             1966..2745
                     /locus_tag="ACG8ER_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS5050884.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKEAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNGKTAPSRRSRSRV"
     gene            2757..3455
                     /locus_tag="ACG8ER_002350"
     CDS             2757..3455
                     /locus_tag="ACG8ER_002350"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS5050885.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLSNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQIFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLSKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3483..4247
                     /locus_tag="ACG8ER_002351"
     CDS             3483..4247
                     /locus_tag="ACG8ER_002351"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS5050886.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFRKNPNYLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIGKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4260..6077
                     /locus_tag="ACG8ER_002352"
     CDS             4260..6077
                     /locus_tag="ACG8ER_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS5050887.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFIQD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            6089..7054
                     /locus_tag="ACG8ER_002353"
     CDS             6089..7054
                     /locus_tag="ACG8ER_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFS5050888.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKVSESNYIEELIKVIKFFEIKLIVPTIDTELSTLSRNKKYIENETDSVV
                     LVSSEKVIDICENKILTSAFFEQNGFKTPEILNSSEITKFPVFIKPLNGSSSVNTFIV
                     NNEKELLFFSEYIDNPLIQEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKG
                     QIIKDRELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGAN
                     SPEYIIKMIDSKKNLRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            7047..7742
                     /locus_tag="ACG8ER_002354"
     CDS             7047..7742
                     /locus_tag="ACG8ER_002354"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5050889.1"
                     /translation="MIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEI
                     YRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTE
                     LRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLE
                     KFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIR
                     SLWEIKNFLGVIS"
     gene            7739..8857
                     /locus_tag="ACG8ER_002355"
     CDS             7739..8857
                     /locus_tag="ACG8ER_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFS5050890.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            8847..9467
                     /locus_tag="ACG8ER_002356"
     CDS             8847..9467
                     /locus_tag="ACG8ER_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5050891.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            9663..10763
                     /locus_tag="ACG8ER_002357"
     CDS             9663..10763
                     /locus_tag="ACG8ER_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5050892.1"
                     /translation="MAKYYNKKILIMANNDDGLYRFRGMLIKNLVDQGNEVIVSLPKG
                     KNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYG
                     GLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQ
                     TLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNL
                     YSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHE
                     GMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYE
                     QRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            10770..11165
                     /locus_tag="ACG8ER_002358"
     CDS             10770..11165
                     /locus_tag="ACG8ER_002358"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5050893.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            11162..>12136
                     /locus_tag="ACG8ER_002359"
     CDS             11162..>12136
                     /locus_tag="ACG8ER_002359"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5050894.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKIGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKNKIINLIYPKLIEDEHGE
                     YFSPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDIIQNLSDEYYTRAICKCKYKDNWKGISAKFALKNKWVFLIY"
BASE COUNT         4475 a         1812 c         2406 g         3443 t
ORIGIN      
        1 aatcgtaaat tgccctagtc aagaaaacag tttgtggtat gattatatag ggaaaacttg
       61 tttacaaata tttctgtgaa tatattagtt taattatgtt ttttaaaata aaaagctgtc
      121 atttttatgt aagtgaatta tatcttaatt tatctatcat caatacttag gagtgaatct
      181 atggaaaagc cattcgtcaa gtcattgact gctgtggcgg taggtgcagg tgctattgcg
      241 atctgtttgt ttggttacca tatcaataat cagcggcaac atcagcaacg gatcaattat
      301 gcagaatctg ccatcaccaa tcaaaaagac accttgactt ctttatcaaa agaagtagac
      361 aagctgtata gtacaaaaga aaaaaatttt ctaaaccctg aaataacaga agaaacaatc
      421 acaagtttgt caaataagct atcctctgtc aaactttcag cagatgatta cgacatcaaa
      481 gaatcagaat tgcctaaaga agctgttgca atccaagatg agaagaaagc aatcgtgaca
      541 cagttagata gtgcagaatc aaaactgaag atccagacag ctgtcaacga tttatttact
      601 aaaaatgtat caaactggca gcaagctgta gacgatgtcg tcatcaaaga aaaggtgaca
      661 agtgcagatg tcgctcgtgt acgtgaaaat atgagctttt ttaaagatag tccttggaaa
      721 acgattgtta tgcaatatct aggattcgct gatactcaga tcgcgcaagt cacgcaacta
      781 gatcagttat tcgatacgat gttgaaggat ggtcaagtga ctgccaccgc tacttacgat
      841 caatatttga cggtgctcag tcaaatagat cagatccgga atgaaaaaat acgcgcagca
      901 tacgcaacaa aagcacaaac tgttgctcag caaatgggct atagcaatat aagctattga
      961 cagttaaaac gttgattcaa gcaaggttag caaacggagg aaactatggc aagacgaaga
     1021 agaaaatcaa gagggaaaaa aatatttctg attattttag cagttcttat cgtattagta
     1081 ttagcaattg taggagtagg agcaaaatta tatctggatc tctcacattc tattgagaaa
     1141 acttacgaat cagtggaacg aacccaagga gaacgaacga aaccagttga tctggacaaa
     1201 caagaatctt tctctgttct attgatgggc attgatactg gagatttagg tcgggtcgat
     1261 caaggaagat cagatacgat gatggttgcc acagtcagtc ctgaagacaa acagacaacg
     1321 atcgtcagca ttcctcgaga tacgtatgtc gacatcgtag gtcgtgacac aaaagacaag
     1381 atcaaccacg cttacgcatt cggcggagct gccatggcga tggataccgt acaaaaatat
     1441 gtagacatcc caatcgatca ctatgtctct atcaacatga aagggctaaa agaacttgtt
     1501 gatgcagttg gtggtatcga agtcaataat gacctcactt tctcacaaga tggctatgac
     1561 tttacaatcg gtaaaattac tttgaacggg gatcaggcat tatcttattc tcgtatgcgt
     1621 tatgaagatc caaatggtga ctatggtaga caagagcgtc aaagaaagat cgtagaaggt
     1681 gtagcgaaaa aagtattgag tctaaacggt gtcagcaact atcaatccgt tttgaatgct
     1741 ctagagtcga atatgaaaac agacatgagc tttgatgaaa tgaaaaaaat tgctttagat
     1801 tatcgggagg catttggtac gatcaagcaa gatcagctgc aaggagaagg cttcatgcaa
     1861 gataatatct cctatcaaaa agtcagcgat caagaacttg atcgtgtgaa aaaagaatta
     1921 aaagatcaaa tgaatctaga gaataaatag gaaagtggag aaaaaatgga agagacaatc
     1981 agcttgaaag aaatttttga aacattacgc aaacacttgg taacaatcat catctcaatg
     2041 tttgctggct tggccatctc aggaatcgta acatttttca tcttgacacc aaaatacagt
     2101 tcacaagcac aattgatcgt gacactgcca caatcagaca cagcaaatgt caatgatgtc
     2161 aatacgaacc tgcaaatgat caatacgtat aaagatatga tcgtcagtga tttagtgcta
     2221 aatgaagtca aagatcgttt agaaacagaa gacaatgtga aaatgactgc tgaacaaatc
     2281 aaagaggcca tctcagtcaa tcagtcacaa aattcacaaa tgttctcgat ccaggcaata
     2341 agcacgaatg cagtgactgc tcaacagatc gctaacacaa cttcacaagt tttccaagag
     2401 aacgcaaaag acgtgatgaa tgtcgataaa atctcgatta tttcaggtgc agttgccaat
     2461 acgacacctg tttcgccaaa taataaattg aatttagtga ttggcttagt attaggaatg
     2521 atggtaggcg taggtctggc attcttattc gaattactcg atcggacagt gaaagatgaa
     2581 aaatatgtga cggatacatt aggattccca atcttaggaa cagttccaga aatgtcagcg
     2641 aaagaattga acgctggtat ccgtaaaaaa ggatcagctc ctgcacgacc aaaagctaca
     2701 aatggtaaaa cagcgccatc acgcagaagc cgctcaagag tctaggagga agaaaaatgg
     2761 cacgaacaca gaaacaaaaa gcagcacaaa ctcatgcagt cagtttgatc acattaacaa
     2821 acccatcttc accaatcgcc gaacaatatc gtacgatccg gacgaatatc cagtttgcct
     2881 ccgcagctgg tcagcaaatc aaaacgattg tcgtgacttc atcaggtcct ggagaaggaa
     2941 aatccaccac tgcagcaaac attgcagtcg tttttgctaa atccggtcaa cgggttctat
     3001 tagtcgatgc agatttacgt aaacccgtcg tctataaaac attccagttg agcaatgcta
     3061 gtggattaag tacagcactt agttcatcag gaagtgtagc agatgtgatc caacgaacgc
     3121 cagtcgagaa cttgtcgatt ttgcctagtg ggcctaagcc acccaaccca tcagaattgt
     3181 taagctcgcc aagaatggat cagattctag ccgaagcaag acagatattt gacgtggtga
     3241 tattcgatat gccaccagta gtagccgtaa cagatgcaca gatcatgtct tcaaaaacgg
     3301 atggcacttt attggtcgta cgtgaaaata catcacgtaa agaatccttg tctaaagcaa
     3361 aagaactgct agagatggta caagcacgag tcttaggtgt tgtatacaac ggagcagaac
     3421 atagcaaaga tgctggttat tactacggaa attaaggaat aagaagagag gagacaagag
     3481 aaatgatcga tctacattgc catatcctac caggaatcga tgacggggca gaaaacttag
     3541 aagcaagtat tgcaatggct gaaaaagcga ttcagcaagg catcacgcat attttatgca
     3601 caccacacca caataatgga aaatacagca atgaaaaatc acaagtcatt tctctagtcg
     3661 cttcgttaca agccgaatta gagaaaagac aattgccttt gacgttattg gaaggacaag
     3721 aagtacgtat taccggaacg ctcatcgaag acatccaccg agatgaaatt ttatttacag
     3781 atttagatga tacatatctt ctgatcgaat ttccgacact ggaagtgccg ttatatgcag
     3841 aacggctttt ttttgagttg ttagaactag gaaagacacc agtgatcgtc catccagaac
     3901 gtaacgcgca ttttcgtaaa aacccgaatt atttgcttcc gttcctagat atggggtgtt
     3961 tggcccaact tactgcgcca agttatgttg gggtgtttgg caaagacatc cagaaaacag
     4021 ctaaaacgat ggtcaaacat aatctcgtgc agatggctgc ttctgatgcc catggggtct
     4081 ctaagcgcac cttttactta aaagagtgct atgagcagat tggcaaggac tttggtaagg
     4141 aaaaagtagt acagatgaaa caagtcgcaa aggatctgat caacggggat gaaatcagtt
     4201 atccagtcta tgaagaggta aaaaagaaga aatttggttt attttagggg gaatgtaggg
     4261 tgtttgtatt aacaagaaga agaaaaattg ccacattggt tgttgtggac agcttgttat
     4321 taatcgctgc aaatattgca gcagctaaat tcatgaagcc gtttgtagct ataccaatgg
     4381 accttatttt aatctctatt ggtttgtcga ttggttttta tttattttat ggtagtttat
     4441 ttaaagtttt cactcgaatc aatcgctata caaatttaag agagatcatt gcaattttta
     4501 gttcactatc tgcaagtgct gcctcaagta tcctcatact gctgttcatt aatcggcgat
     4561 acagtttacg gttagttatc tttgcttatc tgctttcgct cttattgatc atcggtagcc
     4621 gattgatctg gcgtatctac atcgaaacaa aaaatatgag atatgtatca gcagattctg
     4681 ctaaaaatac attgattgtt ggtgctggcg aaggcgggcg tattttatac aacagctttc
     4741 ttggatccaa aacggctcaa gatatccacg ttgtcgggtt cgtagatgat gatccaaata
     4801 aaagaaatac gtatctttca ggaaaaaaag tattaggtgc attaaaagat attccggaat
     4861 tgattgaaaa atatgacatt caaatggtaa cgatcgctat cccttcctta tcaagaaaaa
     4921 aattacgaag aatcttcgag ttagtcgaat ccgctcgtgt gaaagtcaac acgatgcctt
     4981 ctatagaaga acttgcttca ggaaaaatta gcgtatcaaa attaaaaaca attgacgtag
     5041 tggatttact gggaagagat gaggtaaagc tggatattga atccatcaaa gatcagatca
     5101 cagacaaagt cattcttgtc accggcgcag gaggatcgat cggttcagaa atctgtcggc
     5161 aaatcattca attcaatcct gcaaagctat tgcttttagg acatggggag aactcgatct
     5221 acttgattga ccgtgagtta cgcacgcatc atcaaaattg tcctacagaa atcgtaccga
     5281 tcatcgcaga tatccaagat cgcgagaaaa tcaatgagat catggaacaa tatcatccag
     5341 atatcgtcta ccatgcagct gctcataagc atgtaccact gatggaatat aatccaaaag
     5401 aagcagtcaa aaacaatatt ttcggtacaa aaaatgtagc agaagcagct aaagcagcaa
     5461 aagtgaaaaa cttcgtcatg gtatcgacag ataaagctaa caatcctcca aacgtcatgg
     5521 gttcaacaaa acgaatcgct gaaatgatcg tgactggatt gaatgaagaa ggctgtacga
     5581 aattctcagc tgtcagattt gggaatgtcc ttggttctcg cggtagtgtg attccagtat
     5641 tcagagaaca aattgctcaa ggtggtccaa tcactgtaac agactttcgt atgacccgat
     5701 acttcatgac gattcctgaa gcgagtcgtt tagttattca atctggcgca ttagcaaaag
     5761 gtggcgaaat ttttattcaa gatatgagtg aaccagtgaa gattgtagac ctagccaaaa
     5821 acatgatccg attgagcggg tatagcgaag acgaaatcga aatcattgaa acaggtatcc
     5881 gtccagggga aaaactatat gaagaactat tgttggataa ggaaagaaat gatgaagcag
     5941 tttatgagaa aatctttgta ggaaacatca aaggttactc aatacaaaaa gtgatggatt
     6001 ttgttaagag cctgccacaa gacgatgaac aactggctaa agacattgtc acttttgcaa
     6061 atgcttcgaa tagataggag attaaaaatt gaatatttta atattaagtt gtggacgtcg
     6121 ttgtgaacta gtgaatgcat ttaaaacagc attaaacaat aatgaaatgg aaggaaaaat
     6181 cattggagca gattgctctg aactagcacc cgcactatat atgtgtgacg aaaaaatagt
     6241 attacctaaa gtttcagaaa gtaattatat tgaggaatta ataaaagtaa taaagttttt
     6301 tgaaattaaa ttaattgttc ctactattga tactgaatta tcaacattat caagaaataa
     6361 aaaatatatt gaaaatgaaa cagactcagt tgtattagtt tcttcagaaa aagtcatcga
     6421 catttgtgag aacaaaattt taacttctgc tttttttgaa caaaatggtt ttaaaactcc
     6481 tgaaatttta aactcttctg aaattacaaa atttcctgta tttataaaac ctctgaatgg
     6541 tagttcatct gttaacacat ttattgtaaa taatgaaaaa gaattgttgt tttttagtga
     6601 atatatagat aatcctctta ttcaagagta tattttaggt gaagaatata ctatagatgt
     6661 ttgcttaaac tttgattcga caccagtaag tattgttcct agaaaaagaa tagcaactag
     6721 aggtggggaa atactgaagg gtcagattat taaagataga gaactaattg agactacgaa
     6781 aaagatgttg gaaaaattaa atgcaatcgg acatattact attcaatgta taaaaaacga
     6841 aaagggaata ttttatttag aaataaatgc aagatacggt ggaggagccc ccattagcat
     6901 taaatcagga gctaattccc ctgaatatat tataaagatg atagattcaa aaaaaaattt
     6961 gagatacaga gaaaattatt tagataaaat gatagcactg agatatgatc aagcgattta
     7021 tttagatgaa aaaggaaatg tatataatga ttaaagcggt gatctttgat ttagatgaca
     7081 ctcttgtgtc tgaatatgat tatgtaaaaa gtggatacaa atgtgtagct agaagattag
     7141 cagtgcaaaa aattttcccg gctacttttg acgaaatata tagagtcctt ttagatcttt
     7201 ttgctgaaga tagtaaaaat gtatttaatc gtctatatga ctattttgaa attccttata
     7261 gtaaaaaaga aattaaagaa ctggtaaatg tatatcgaga acacaaacca gacataaatt
     7321 ttttagaaaa tgcagatact ttattaacgg aattaagaag tagaggatac aagctgggga
     7381 ttattacaga tgggtatgct ataacccaac gaaacaaatt ggatgctttg aaagtagact
     7441 atttggtaga ttatatttgt attactgacg aattaggccg tgaattgtgg aaaccacacc
     7501 ctatctcata tcttttattg ttagaaaagt ttaatttaaa ggcacaagag tgtatatata
     7561 ttggcgataa tgagttgaaa gattttataa ctgccaaatc aataggaatg caaacaatta
     7621 aaatagaaag agtaaatggt atttacagag aaagcagttt gaagttcaaa agattttacc
     7681 aagctgataa aaaaatacgt tcgttatggg agataaaaaa ttttttggga gtgatttcat
     7741 gaaaagtaaa agaattctat tagcttctcc acatatgagt gaagaaggat atgaaaaaga
     7801 gtatgtaaaa gaagcatttg atacaaattg ggttgctcct ttaggtgcga atgtgaatgg
     7861 atttgagaaa gaattagcag aatacgtggg agctagtcat gctgccgcat tatcttctgg
     7921 aacagcagct atacatttag ctttgaaagc ggctggggtc ggtaaagatg acatagtgat
     7981 ttgtcagtcc ttgacgttct cagctactgc taatccaatt ctctatcaag gagctatacc
     8041 tgtatttgtt gatagtgatt acgaaacttg gaatatgaaa cctgaacatt tgcgaacagc
     8101 actagaaaaa tatcctaaca caaaggctgt attagtagta catttgtacg gtttatcagc
     8161 taatttagac gaaattacta aaatatgcaa agaatacgat gttactctaa ttgaagatgc
     8221 tgcagaatca ttaggaacaa catataaagg aaaatatact ggaacatttg gcgactatgg
     8281 aatttattct tttaatggaa ataagataat cacaacttca ggtggcggaa tgcttgtatc
     8341 caatgacaaa gaaaaggtgg aaaaagtgag attttgggct acgcaatctc gtgagaaggc
     8401 acgccattat caacacaatg aaattggcta taattatcga atgagcaata tcgttgctgg
     8461 tatcggtcga ggacaattga aagtattaaa tgaaagaatt gagaaaaaac gatatattca
     8521 tagtttttat aaaaaagaat tgtctaaatt gtcaaatata caattcatgc cagaaaataa
     8581 ttggaatgaa ccgaattatt ggttaagtgt cataacaata aagggaataa atgcgaatca
     8641 tcttattgat gagttagaca aaaataatat agaagcccgc ccagtatgga aacctatgca
     8701 tttacaacct gtttttaaaa attatgatta tattggcgat gatgtagcag aaactttatt
     8761 tgacttagga gtatgtttac catcagatac aaagatgaca gatgaagatt taaaatatgt
     8821 agtatctacg attaaaagag tgatcaatga tgcgtaaaaa aactttctat cagctttttg
     8881 ggaaaagaat tttagatatc ctattgtcag gaattgctct catcgtttta tcgccgataa
     8941 tcttaattgt aggtttttta gtaagaatta aacttggttc tccaattatc ttcaaacaag
     9001 aacgaccagg aaaaagtgaa aaaatttttt ccatgtataa atttcgtaca atgactgacg
     9061 aaagagacca caatggagaa tatttacctg atgaaattcg actaactaaa tttgggaaaa
     9121 tgcttagagc aacaagtctg gatgagctac ccgaactttg gaatattttg aaaggtgata
     9181 tgtcgatcgt aggacctcgt ccattattag tggagtattt gccactatat agtgagaagc
     9241 aacggaaaag acacaacgta agaccaggat taactggact ggctcaggtc aatggaagaa
     9301 atgctattag ttgggaagaa aagtttgatt tagatgttta ttatgttgat aaaattagtt
     9361 tctttaatga tcttattatt ataattcaga catgtaaaaa agtaatcaag aaagagaata
     9421 ttaatacaat caataacaat atcccagaaa aatttaaagg gagttaaata cagcagctta
     9481 atgcaagttt tgtgacttgc attatattta ggtataaatc aaagcgagta tattagaatt
     9541 atataacata tgttaaaaaa acgtatagct aataaaagta tgattttaaa aatttgtgta
     9601 gagttttaaa tatactgttt caatacgaga aaattataaa aaagtataag gaaaagagag
     9661 ttatggcaaa gtattataat aaaaaaattc ttataatggc aaacaacgat gatggtcttt
     9721 accgttttcg aggaatgctc ataaaaaact tagtagatca aggtaatgaa gttattgtat
     9781 ctcttccaaa gggcaagaat attcctaaat taattgaact tggatgtcaa attattgaaa
     9841 ctccaataga tagaagagga attaatccaa aaactgactt taaactattt tttaactatt
     9901 gtaatatttt gaaaaaaata aagccagata tggtcattgc ttatactatc aaaccaaata
     9961 tatacggtgg attggcatgt cggttaagaa aggtttctta tgcattaaat ataaccggat
    10021 taggaacggc atttcaaaaa aagggggttt tgaaaagtct cattgtattt ttgtatagat
    10081 tagcagctaa aaaatctaag gtcatatttt tcgaaaattt tgaaaatatg aaaacatttc
    10141 agactttaca aattgctaaa caaagtaaat acaagctatt aaacggtgca ggagtagatg
    10201 tagaacactt tagttattta gaatatccta aagatgagca agttcatttt ttatttattg
    10261 gaagaattat gaaagagaaa ggtgtagatg aactttttga agcaatgaaa aacttatata
    10321 gtgaagagaa aaattgtgta cttgatgtcc ttgggtattt tgaagaagat tacggcataa
    10381 aaattgaaaa atacgaaaaa gagggatggt taaaatatca tggatatcaa tctgatattc
    10441 gtccctttgt agaaagagca cattgctttg ttttgcctag ttggcacgaa ggaatggcaa
    10501 atactaacct tgaatcagca gcatcaggga gaccaattat tacaagtgat atcccagggt
    10561 gtaaagaagc agttattgaa tcgaagtctg gttatttatg tgaaagaaaa aatagttgta
    10621 gtctctataa ttcgatgaat aaatttctaa aattatcata tgagcagcgc attaacatgg
    10681 gaaaaatggg gagaaattat atgattaaaa aatttaataa gaagaaagtt gtcgatgaaa
    10741 caattaaatc attatttagt taaaaagata tgttttgcaa agtatgtgat agaaaagagg
    10801 gattctgtgg cttcagaata gatatcaaag aattggcaaa agtttctgat atttttttat
    10861 ttactacatt acaagaagga atgccaagat ctatgatgga agctatggca agtggattgc
    10921 catgtatagc aagtaaaatc cgaggtaatg tagacctttt agtggaagga aaaggtggat
    10981 atcttgttcc agctaaagat gcagaattag tagcagagtg tttgagagaa ctaatcaata
    11041 ataaatctct tagaaagtat atgggaaatg agaacctttc cagaattaaa gaattcgagg
    11101 ttagtatcgt aaaaaaaaga atcaaagaaa tatatgaaga agttataggt gagagtgtaa
    11161 aatgaaaaat ccaatgtttt caataatagt acctatctat tgcgttgaaa aatatttgaa
    11221 agattgctta gattcaataa gaaatcagac atttaaaaat tttgaagcaa tactagttga
    11281 tgacggtagt cctgacgaat gcccagaaat atgtgactat tattcccaaa tagatggacg
    11341 tttaaaagta attcataaga aaaatggtgg aataatagat gcacggaaaa taggagtcac
    11401 tagtgctatg ggagagtata tagtttttct agatggtgat gattggcttg caaatactta
    11461 tttagaaaaa tatgcggaag taattactaa gtacaatcca gatataatat gtagtgggta
    11521 ttttgcatca cgttccaaca aaattagaaa aagagaattg tcttgctcgt ttggatatta
    11581 tgacaaaaat aaaattataa atttgattta tcctaaatta attgaggatg aacatggaga
    11641 atattttagt ccacaattat gggcaaaagc ttttagaact actctgtaca aggagcaaca
    11701 acaagtatct gggcaagtta gtgttggtga agatcatgca tgtacaaaac catgtattta
    11761 ccatgcaaat tcgatatata tgatggaaaa atgtttatat tattacagaa taaacgaaaa
    11821 ttctataaca aaaaaaccac aaccatatta ttgggaagga ccaaaggcta ttggagagca
    11881 ttttgaaaag cagattgata tgaataagta tgattttcaa gatcaagtat atagaaatgt
    11941 tacacacaat ttatttaatg ttgctgtttc acaatttaat aaacaggaca aatatttaaa
    12001 tataaaaaaa gatattatac aaaatctaag tgacgaatac tatactagag ctatttgtaa
    12061 atgcaaatat aaagataatt ggaaaggaat tagtgctaag tttgcattga aaaacaaatg
    12121 ggtattttta atttat
//
LOCUS       DAZUMO010000081        12093 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000081 DAZUMO010000000
VERSION     DAZUMO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.081"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..191
                     /locus_tag="ACG8ER_002360"
     CDS             <1..191
                     /locus_tag="ACG8ER_002360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050895.1"
                     /translation="MLEEKSLNTYNYFISEKMLSVVSPIAEESHSRDYLIKKYVYSIH
                     LFIFALLTYCNNVSNNVF"
     gene            complement(533..1294)
                     /locus_tag="ACG8ER_002361"
     CDS             complement(533..1294)
                     /locus_tag="ACG8ER_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5050896.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            1394..3115
                     /locus_tag="ACG8ER_002362"
     CDS             1394..3115
                     /locus_tag="ACG8ER_002362"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050897.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            3130..4914
                     /locus_tag="ACG8ER_002363"
     CDS             3130..4914
                     /locus_tag="ACG8ER_002363"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050898.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(5295..6848)
                     /locus_tag="ACG8ER_002364"
     CDS             complement(5295..6848)
                     /locus_tag="ACG8ER_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS5050899.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7196..9610)
                     /gene="leuS"
                     /locus_tag="ACG8ER_002365"
     CDS             complement(7196..9610)
                     /gene="leuS"
                     /locus_tag="ACG8ER_002365"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS5050900.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     misc_binding    complement(9761..9967)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10037..12055)
                     /locus_tag="ACG8ER_002366"
     CDS             complement(10037..12055)
                     /locus_tag="ACG8ER_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5050901.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
BASE COUNT         3507 a         2443 c         2181 g         3962 t
ORIGIN      
        1 gaatgttaga agaaaaatct ctaaatactt ataattattt tataagcgaa aaaatgctat
       61 cggtagtatc accgatagct gaggaaagtc acagtaggga ctatttgatt aaaaaatatg
      121 tttattcaat ccatctattt atttttgcct tattaacata ttgtaataat gtaagcaaca
      181 atgtatttta agtgtaatgc ttttatgcac ctatttaatt tatcattctc atctgataca
      241 atctcactca taacaatatg aatttttctt ctcattagtc tcagttcacc atgaattata
      301 cataacaccc aatacaatta tttttccata cttttttaag aggacaatta catcgtatat
      361 atcgctaaga attgcaacca ttccattttt aaagacatat ttcctctaaa aaactaaaat
      421 tctatctatc cacgactaat aatcccccac attaaacccg taatcgaacc agaaaatttt
      481 tctttataac taaaacaacc atcattttcg agttatctta aacacatatt tcctattttt
      541 tcatttctat ccatgcatga taatattcag ttctgatatt gttttcatcc gtattcgtca
      601 atagatactg gccgatcact tctcctgttt tccatccttt ttcatacatt ctttctctga
      661 tttctaaaat attattacgt tccgcatggt tcatctcgga tttcaataag aatcgacgat
      721 aaacagccgt atcttttttc tcccaaatca cgtcttgagt aaataaatgt acttcactag
      781 ggtcgataaa aaatccatac atcgtctgtg aactggaagc tggcatactc atcatcccaa
      841 agcttgaata gttaggtaga aattttttga tttccacaat atcttcaaac tccgttgaga
      901 tcatacagtc aaaagggatc tcttctacag gaaattcttc ctcgtttgta tcgatcatca
      961 atttcaactg tttttttcgc tcccttatct ctttttgttt ttgtttcatc accaccagtt
     1021 ctttatggat tcttctttct gtttcatcaa gtgtctcata taactccgtc aatgtttttc
     1081 catataaatt cttcatctgt ttgatcggta tatctaattt tctataaaaa acgatatcat
     1141 aaagcataaa aatatcatcg atatcaaaca atcgataatc attctcttct tgtctacttg
     1201 aacggattaa tccttgttca tcccaatagc ggatcgttga ttttggcaaa tcaaatagtt
     1261 ccgatacttg gccaatcgta tacatttttt tcattttttc tcacctcttt ttcagtttat
     1321 tcttttttct attgaccttc aagttacttg aaggtataga atcgaaaagt atcaggaaaa
     1381 aaggaggaat aatatgtcta tcttaaaaac attcttacag aaaaacaaag gactcgctct
     1441 ccttaccttt gtgatgatct gtttacaaat cgccggtaca ttaggtgtac caaaattagt
     1501 agcaaagctg atcgacacag gtatagcaag tggcgaagcc tcagttataa aaaaaattgg
     1561 agttgaaatg ctgcttgtag cgtttctagg aacaatcgct gcagttgcct ctagttactt
     1621 ttcagcattg gcttccgcta ggtttggatt ccaaacgcgg gagaaatatt tccgtaaatt
     1681 ccaacagctg tcaatgaagg atatcagtcg ttttagttct ggatctcttt tgacaagaat
     1741 gacgaatgat gtcgataatg tccagcaaat gattgtttta ttttgccaaa tgatcttacc
     1801 agcaccggtt atttgcgttt tcaccattct catgatgttt cgttattctc tattactaac
     1861 atgggttaca ttgatctctg ttgtttttta tgtatggatt gtttatcgac tgatgaaacg
     1921 aggggcacct ttatctttga gtatccagcc aaaaatggat cggatcacca tcacattgag
     1981 agaattcttt actgggatta atatgatccg tgcctttgat aatcaatcat atgaagaaaa
     2041 gcgaacgaat aagagctttg ctgactatgc taatcaaatg atcaaagtga accgaatatt
     2101 tgcttgggtc actccaattg cttttttgct catgggaatc gtttatgctt ctattttgtg
     2161 gtttggcgga aatcttgtag cagaaggagc attacaaatc ggtgtcgtga ctgctgtagt
     2221 cgaatactct atgctgacac ttgcttactt gatgatcgca gctatggtat tggtcattat
     2281 ccctaaatcg tttgcctcat tacgacggat agaagaaata ctgcaagcag agatcgaaat
     2341 tgaggaagag acgatcaaaa cagtagctga accaaccttt gatcagacaa atgcagctgc
     2401 ttttgaacat gtcacattta aatatactga aatctctgat ccagttctag aagatattga
     2461 tttcacaata cctaaaggaa aaacaacagc gatcgttggc gggaccggat ctggaaaaag
     2521 tagtttggcg aaacttctct tgagacttag tgaccctacg ataggaaaag taacccttgg
     2581 tggtattcct cttactcaaa tgactcaaga acagatccgc tcccacatca gttatgtccc
     2641 tcagaaggct ttcttattca gcgggacaat tttaagcaac ttgcagatgg gaaatgaata
     2701 tgcctcaaca gaagagttgt ctaaagcgat caagatcgct caattagaag aactagtgga
     2761 cacccttcct gacggattgg acagctttgt tgctcaaggc ggttctaatt attcaggggg
     2821 ccagaaacag cggatatgta ttgctcgagc gttgatcaaa ccagcagaga tctatgtatt
     2881 cgatgatagt ttctccgctt tagactataa aacagatgca gcattaagag cagcccttca
     2941 taaacacatg tcggagaaaa ctctcgtgat tgttgctcaa cgcctcagta caatccaaca
     3001 agcagataac atcattgttt tagatgaagg aaaaatcgtt ggacaaggta ctcatgaaga
     3061 actactgtct aattgccaaa cttatcagga gtttgcaaaa tcacaaggaa tatagaaaga
     3121 agggatacga tggaaaagaa aaaaacgttg aacacgcgtg ctttgatacg tttttggaaa
     3181 atgatcaaac ctgaacataa atatttttac ggattgtttc tatgcagttt attcgggaac
     3241 ctattagtcg tagccatgcc cttgatcatg ggaatcggga tcgatgatct cttatcagaa
     3301 ataagaaaaa caggtattgg acagctttct ttcagtgata tcaaacaagc attgttgttc
     3361 cctgtcttat tgctgatcgg ctgttcagtc ttaagcagca tcacttcatt tattcaggaa
     3421 cggacaatgg cagcgttaag cgaaaagatc acattgaggg tgcgaaaaga aatcacaaaa
     3481 aaattcaaaa cattaccgat gtcgtttttt gacaatcatc aagtaggtga catcattagt
     3541 cggacaacta ccggtctcaa tcaactttct caagtgttat tgacaggtat caatcaattt
     3601 tttacttctg tcgtaacaat agctgctgct ggtctgatgc tgttttatat cgaaagcaag
     3661 cttactttgc tcgtcttatt gctgatcgtt ggcagttcaa ttgttacaac taagattgcg
     3721 aacaaaaaca aacagatttc tgaacagaat cagtatgaac tgggtgtttt aaataatcga
     3781 atggaagaaa tcttagctgg taatctagtg gtcaaaacat tcaatcagca agctcatgca
     3841 gaagctgcta tacacgaagt aaataaaaag caatacaacg cttttaaacg tgctcaattt
     3901 atgaactttg ctatctatcc ggccattcgt ttcattaatc aactcgcctt catcataagt
     3961 gcggtattcg gtgctatgct tgttctatca ggtggtgtga cgatcggctt cttacaagct
     4021 tatcttcagt acatcaatca gatttctgaa ccaatttcta ccgcttctta tgtgatcaac
     4081 tcgatccaat ccgcaatggc agcaatcgat aggatatttg agatattaga tgaaacagat
     4141 gaaattccag accttccaga tgcaagactt ttagaacaac cgaaaggaaa aatagctttc
     4201 aaaaatgtac agtttggtta cacatcagat aaaatattga tgaatcaagt cgatttttca
     4261 gtaaagccaa aacaaacggt agcgatcgtt ggtccaactg gtgcaggaaa aacgacttta
     4321 gtcaatctgc tgatgcgttt ttatgaactg aataaaggaa agatcgaatt cgacggtctt
     4381 gatattacca agctttcacg tggagaacta cgtcgttctt ttgggatggt tttacaaaat
     4441 acctggctgt ttgaaggaac ggtagctgat aatatcgcct acgggaaaat ggatgccaca
     4501 agagaacaaa ttatcgaagc ggctgaaatc gcacaatgtg atcactttat tcgaacactg
     4561 ccagacggat atgatacgat catttctagc gaaaacggca cgttatctca aggacagcaa
     4621 caattattga caattgctcg agtcattcta gcgaatcctc ctgtcgttat tttagatgaa
     4681 gcaacttcta gcgtagatac acgaacagaa gcattgattc agcaagcaat ggaagctgtg
     4741 acaaaagata ggacaagttt tgtgattgca caccgtttgt caacgattga aaatgcagac
     4801 atgatccttg taatgaagaa tggcgatatc gtcgaaaaag gaacacattc tgaactactc
     4861 caatcaccaa cgctttatgc cagtctttat aatagtcaat ttcagatagc gtaacaaaca
     4921 ttatctaaaa agctatcgat catgtatgat tagtccatga tcgatagctt ttttaaatgt
     4981 actagtggtg ccactagaaa cacaaagaat aatactaatg atactttttg gatagctcct
     5041 tcagtttcgt tcggtaagta gtgacaaaag acttgtcaca atcccttttt acgaataaac
     5101 ggtgttcaag agacaaccta tcggcaataa gacacgatct tgtaaaatac gagaagctat
     5161 acttcaaaat cgcttcttgt tgctcttgtc aactccgaat cacgagttac actattcaac
     5221 aaatatgaga agctgttttt tgaatttgtt tcttgtgact cggtgctgaa cgtcaggctc
     5281 acaatctctc ttctttaatc tccttgttct acttgcgata gttgctgaac gaattgccat
     5341 gtttccccgt tatcagttga acggaagagg caggcaaaca ttttccctgc atcgattggt
     5401 ccttttttca acaaataaaa ttttgtctct aacgtattcg cgcttatttg agtaacttca
     5461 tttggtgatt gaaaaagatc tatcccccct gtctgatagt tttccggaat cgacagaact
     5521 gattctttga atgagcgtcc tccgttgttt gtataaaaca aactttcacg tgtcgaaaca
     5581 aaacctaaag aagttgtaac gaagctgaca ttttgtactg gctgattgat cattgtactt
     5641 ccacttttta tccaagtgat tccgccatcc atagtagtgt aaatgtacaa agtttctgca
     5701 gacatcccac tggctgatgt acagataaat agaccatcgt tgccataaaa taccgctttt
     5761 cgataacgta tcggctgaac tgattgggtg ataatagacg tttgccatgt tttaccatta
     5821 tctcttgaag tcagcagacg tacatcatcc ccagaagaat agataaacca tgagaaatcg
     5881 gcggaaatat catatgtgtt gtccatccaa taaccttcag gaatcagccc cgttgtcagg
     5941 gtgtattcgc caaaacgcaa ccattgcgaa tcaatcggca caaataccca attagcacca
     6001 ctatctgttg tcatatagag agagtgcgtc tctgtatcaa gtttgagatc atttatcttt
     6061 tgtgttgtca aatttacctt ttgcttagga tcagctaaca acccttcatt tgtcttttgt
     6121 atatgcgtat cgttagttga gttcattgtc atgtgagaag cagcatttat atgagaagac
     6181 atttttggct gagtgtatct cccataatag attgttccag ctaaaagaat gaggaaaaaa
     6241 ccagtgacta atgtactcat cttagttgtc aggtatccac caggcgaaga ctggcttttt
     6301 tcccatctgg gttcgttttc tagtaaaaaa atatggaatg tgttcattcc gaattttgat
     6361 ttgctttcgc ctaagtagat aggtaaatca tcaagtgatc tcagcaataa ctggcaatca
     6421 cttttttgta tccgaaaagc ttgactcgtc gaagtcttct caaaataaag atagtcatca
     6481 tcttgagaag ttggtttatt atgtaaaaac tgttgtgctc tttgactaag ccattgatcg
     6541 aaatgacgcg acaattgatg gtaacggagc caaataagta gaagaaaaac aataagcaca
     6601 acaattgtta ttccgccaac tccccacgaa aagtgataca aattatttaa tttttgcatc
     6661 ccttcagctg aacggactac tgcactcata tggcgatata ggaacgatac agctccgaaa
     6721 aaaagcaaac cagagaaaaa tactaagctt aaaagcttga acattattct tttcgctctt
     6781 tttttcgctt tctcgataat tttttgttta gctgataata cttcgataat aaatagttct
     6841 tgatccattt gtctctcctt ttctcatact tcattcatta tgcgtcaaaa tccatacaat
     6901 cactagtccc actaataaaa aatttatgaa tttggtatgt actctcaatt tcaggtagtt
     6961 tatacatcaa aaaatactaa gattaataga caaaaatttg atgattacag agatctctta
     7021 caagaccatt cagaagaaaa agacattgaa cgtgtttgag aacatgaaaa tcaaaacctt
     7081 tttgatcaat caattaatca atttgaaata acaaaaaatc tgagacagaa tgaagttcat
     7141 tctggcccag atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaattaat
     7201 tagcaacgat attgactaac ttgtttggta ccgcaataac ttttctgact gttttgcctt
     7261 caatatattc ttgcactatt tcattggcta atgctgcttt ttctagctca tcttttccta
     7321 agtctcgggg aacattgctt ttcgcacgaa ctttaccgtt tacttggaaa acaacttcta
     7381 cttcgtcttc aacaagtgct gcttcatcgt atgttggcca tggtgcgtaa gaaataccac
     7441 catcatttcc aaggattgcc catagttctt cacccatgtg aggagcaatc ggtgcgagta
     7501 gttgaacaaa tccttcgata tattcataag gcagagcgtc cactttatag gcttcattga
     7561 taaagaccat caattgggag attgctgtat tgaaatgaag attttcatag tcttctgtca
     7621 cttttttcac tgtttgatga tacactttat caagcttgcc atcattcaat gtagtgatac
     7681 ggtcacgcat tttattgttc tcatcaacga tcaaacgcca tacacgatcc aagaatttac
     7741 ggcttccttc taagccattt tcgctccaag caatcgaagc atctaatggc cccatgaaca
     7801 tttcgtacat gcgcagtgta tctgcaccgt aagtttcgac cacatcatca ggattgacta
     7861 cattaccacg agattttgac attttttcgt tgttttcgcc taagatcatt ccttgattgt
     7921 atagtttttg gaatggttct tttgtaggaa caacaccgat atcgtataag aatttatgcc
     7981 agaatcgtgc atatagtaaa tgaagtaccg catgttctgc tccaccaatg tagatgtcaa
     8041 ctggtagcca acgcttcaac ttttcataat ctgctagttc ttttttgtta tgagggtcga
     8101 tataacgcag gtaataccat gagcttcctg cccattgtgg cattgtattc gtttcgcgac
     8161 ggcctttttt acctgttaca ggatcaacga cattgaccca ctcctcgata tttgctaatg
     8221 gagattctcc agtaccgctt ggcttgatgt tttttgtctt aggcaaccgt aatggtagct
     8281 cttcttctgg gacagctgtg actgttccgt cctcccaatg gatgattggg attggttcac
     8341 cccagtaacg ttgacgggag aatagccagt cacgtaagcg atagctgact tcttttttcc
     8401 ctacgccatt ttcttctagc cattcattca ttttatcgat tgcttcttgt ttgttcatgc
     8461 cgtctagaaa ttcagagttg atgtgcgtac catcttctgt gtaagcagct ttttcaacat
     8521 ctccaccttc gataactgga agaatttcta gtccgaaagt ttgagcgaat tcaaagtcac
     8581 gttcatcatg tgctggaaca gccatgatgg ctcctgtgcc gtatgaagat aatacataat
     8641 cggcgatcca gattgggatt tccttgccat taactggatt gattgcataa gcaccagtaa
     8701 atactcctgt tttttctttt gcaagatccg ttctctttag atctgatttt ttagctgtct
     8761 cttcgatata agcttctaca gcagcttttt gttcaggggt cgtaatttct ctaacaagat
     8821 ctagttcagg tgcaagaacc gagtaagttg caccgaatag agtatctggt cgtgtcgtaa
     8881 atacggtata ggatttatcc gtaccagcca ctttaaattc aatatttgct cctactgaac
     8941 gaccgatcca atttctttgc atttctttaa tgctttctgg ccaatcaact aattctagat
     9001 catccaataa gcgatcagca taagcagtga ttttcagcat ccattggcgc attggttttc
     9061 tgactacatc atagccacca cgttcacttt tcccatcgat cacttcttca tttgaaataa
     9121 ctgttcctaa ttcaggtacc cagtttaccg ctacttctgc ttcataagct agaccttttt
     9181 cataaagctt cgtaaagatc cattgtgtcc atttataata ctccggatca gttgtattca
     9241 cttcgcgatt ccagtcataa ctaaatccta aagaattgat ttggcgtcgg aatgtttcga
     9301 tatttttttg ggtaaactca gctggatcgt ttcctgtatc tagcgcatat tgttcagcag
     9361 gcagaccgaa tgcatcccac cccattggat gcagcacgtt gtatccttga cttcttttca
     9421 cacgtgaaag gatatccgtt gccgtataac cttcaggatg ccctacatgt agcccttgac
     9481 cggatggata gggaaacata tccagtgcgt aaaattttgg cttttcagga tcatcatggg
     9541 tgttgaaagt attcgttttt gcccagtatt tttgccattt tttctcaatt tctttatgat
     9601 tgtaactcat ttgctgtcct cctgttctat tgaaaacact tgtttctatt ataaatataa
     9661 cgaaaaaacc ggcagaatct gtttattttt atttcctttt gctttgtttt ctcaaacttc
     9721 acaaaagaaa taaaaaaatc ctataaaaag attgctcttt ttataggacg taatctacgc
     9781 ggtaccacct aattttacct tagtgaaaag gcctcgaacg ttgtaacggc cgtcaccgcc
     9841 gcttgcttac tcgggtattc agcaagggca actcaaaggc gagttcaaaa gcatccgtgc
     9901 acattctcac cagccatgcg ctctctgaaa cttcagtctt tttactactt ccttttcaag
     9961 tgttgtcata tttaagtagc cttatagtaa caaatatttt tgtggaattc aatcgttatt
    10021 tcttcgaaca gctattttat ttagctacaa tcaacgtttg acctggataa ataaagttgt
    10081 tcttaatatt gttccattcg attagttgag ccattgtaat tccatgactg tcagcaacac
    10141 tccaaacaga ttctccagat ttcacagtat aggttttcga accagtaact gattgttggt
    10201 ttgttgattg agatgaacct ttcttcacaa tcacttgctg tcctggatag atgaaattat
    10261 ttttgatatt attccattcg attaactgat ccatcgtgat atgatgtttg ttcgctacgc
    10321 tccaaactga ctcgcccgct ttcacggtat acatcgtatt accagtggat gatgtacttg
    10381 tcgtagcaga cgtattcggt gtatttggtt tcgctgcaga agtagttgaa cctgtatttg
    10441 tatttgccga agttccacct ttaacgatca attgctgtcc tggataaatg aagttgtttt
    10501 tgatattatt ccattcgatt aactgatcca tcgtgatgcc gtttttattt gccacgctcc
    10561 aaacagattc acctgctttc acggtataag tgccttgaga cgaactgctg ccagtattaa
    10621 ctgaactgct gttatttgta ttggttgacg aacctgtatt acctgacgtg ctgtttgttg
    10681 atccactgtt tgaaacagtc agttgttgtc ctgggtagat aaagttattt tggatattgt
    10741 tccattggat tagctgagcc atcgtaattc cgtatttatt tgagacgctc caaacagagt
    10801 ctccggcttt aactgtatat ttcgttcctg aagctgtatt agaagaactt gagctggacg
    10861 aactagagcc acttgtattc ccagtatttg agccagtatt tgctccagca ttagaagaac
    10921 cgccatttga tacgattagc tgttgtcctg gataaacgaa attgttcttg atattgttcc
    10981 attcaatcaa ttgagccatc gtaattccgt gatcatttga gatcttccaa acagaatcac
    11041 cagattgaac agtgtatttc gcaccagttg tttgtcctcc gtttgatgta ccagcatttc
    11101 ctgaagaatt ggtattgtta ccggaattat tcgtcgaaga gccattttcc tggctacctt
    11161 tgattgtcag tttttgtcct ggataaatga aattgttttg gatgttgttc cattggatca
    11221 attgatccat tgtgataccg aatttatttg agattcccca aacagaatca ccagattgga
    11281 cagtataagt ggtcgaagtt gctgaacctg agtttgtatt accagaattg cttgaaccgc
    11341 cagtactacc tgcacttcca gtattattgc cacctgtagt cgctccaccg cctgcgtttc
    11401 cgctagctgg tgtatcatat tgggtcaatc cgtaagttgc gatgatatta tttaatttgc
    11461 ctgcgtaacc aggatcagtc gcataacgcc ctgtcaaaca agctgtcgca tctgtatagg
    11521 atttcgtgtt gctcttccaa gtaccggcat agtaataacc gttgccaaat gaagtatttc
    11581 taagtacata ggcattgtca ttgaaagatt cagcaaaaga aggatattgt cggaaaggct
    11641 cctttttatt tacccattga ttatttaaaa attcaagcgt atccatgtag acggtttgtc
    11701 cttgatagct gccttttatc ccaaaaagat tataataagg tgcttgagat aacgcactac
    11761 tgccccaacc gctttctaca actgcttgtg cgatcatcac tgatgcatat agatcgttgg
    11821 cttgagcgat tggttgagca taagtagcaa tttctgtgat aaatgccgct gtattgattc
    11881 ttgcgccgaa ctgaacaggt gtttcatccg cttgaacagg aaccggctgt actaatgggg
    11941 cagcgacaga acagcctacc atcgcagttc ccacgattgc tgcccctcgt tttgcttttc
    12001 gaaaacgttt ggtctgttct aaacgtctcc gttcttttct tgcaatgttt tccatcttct
    12061 atcctccaac ttcaatttat ctattattaa taa
//
LOCUS       DAZUMO010000082        11220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000082 DAZUMO010000000
VERSION     DAZUMO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.082"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG8ER_002367"
     CDS             136..309
                     /locus_tag="ACG8ER_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050902.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACG8ER_002368"
     CDS             949..1422
                     /locus_tag="ACG8ER_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS5050903.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACG8ER_002369"
     CDS             1431..1658
                     /locus_tag="ACG8ER_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5050904.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACG8ER_002370"
     CDS             2294..2665
                     /locus_tag="ACG8ER_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050905.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACG8ER_002371"
     CDS             complement(2921..3163)
                     /locus_tag="ACG8ER_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5050906.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACG8ER_002372"
     CDS             complement(3195..4097)
                     /locus_tag="ACG8ER_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5050907.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACG8ER_002373"
     CDS             complement(4110..4298)
                     /locus_tag="ACG8ER_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5050908.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG8ER_002374"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG8ER_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5050909.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACG8ER_002375"
     CDS             complement(4913..5806)
                     /locus_tag="ACG8ER_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5050910.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACG8ER_002376"
     CDS             complement(5884..6807)
                     /locus_tag="ACG8ER_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5050911.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACG8ER_002377"
     CDS             complement(6856..7206)
                     /locus_tag="ACG8ER_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5050912.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACG8ER_002378"
     CDS             complement(7239..8141)
                     /locus_tag="ACG8ER_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5050913.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACG8ER_002379"
     CDS             complement(8134..8991)
                     /locus_tag="ACG8ER_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5050914.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ACG8ER_002380"
     CDS             9325..10134
                     /locus_tag="ACG8ER_002380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5050915.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ACG8ER_002381"
     CDS             10174..10671
                     /locus_tag="ACG8ER_002381"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS5050916.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ACG8ER_002382"
     tRNA            complement(11056..11129)
                     /locus_tag="ACG8ER_002382"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
BASE COUNT         3294 a         2097 c         1879 g         3950 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
//
LOCUS       DAZUMO010000083        11147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000083 DAZUMO010000000
VERSION     DAZUMO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.083"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8ER_002383"
     CDS             6..305
                     /locus_tag="ACG8ER_002383"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5050917.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8ER_002384"
     CDS             378..1739
                     /locus_tag="ACG8ER_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5050918.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8ER_002385"
     CDS             1761..2021
                     /locus_tag="ACG8ER_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050919.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8ER_002386"
     CDS             2035..2787
                     /locus_tag="ACG8ER_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS5050920.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8ER_002387"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8ER_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5050921.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="ACG8ER_002388"
     CDS             complement(3542..4516)
                     /locus_tag="ACG8ER_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS5050922.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIK
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="ACG8ER_002389"
     CDS             complement(4894..5613)
                     /locus_tag="ACG8ER_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5050923.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACG8ER_002390"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACG8ER_002390"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5050924.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="ACG8ER_002391"
     CDS             7164..8561
                     /locus_tag="ACG8ER_002391"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5050925.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="ACG8ER_002392"
     CDS             8642..9082
                     /locus_tag="ACG8ER_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5050926.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9228..9554
                     /locus_tag="ACG8ER_002393"
     CDS             9228..9554
                     /locus_tag="ACG8ER_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5050927.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9555..10304
                     /locus_tag="ACG8ER_002394"
     CDS             9555..10304
                     /locus_tag="ACG8ER_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS5050928.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10330..10740)
                     /locus_tag="ACG8ER_002395"
     CDS             complement(10330..10740)
                     /locus_tag="ACG8ER_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5050929.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3536 a         1947 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagttttgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tctttgcttt ctgttccttt aattttttgt aaaaaattac tttacctgat gaagtaatca
     9181 tgctattcta attacaacga cagatggagt aatatggagg aaagaaaatg atttcaagtg
     9241 atggaatacg cggatacaat gatgcgatta ttttatcgat ccttaacaaa aacgattctt
     9301 atggatatga aatttctaaa acgattcgtg agaggacaaa tgattcctat gtgatcaaag
     9361 agacaacact ttactccgtt tttgcccgtt tagaaaagaa tcaactgatt gtttcgtatc
     9421 ctggagaagt gactcatgga aaaaagcgga catattataa aatcactgaa atagggcgta
     9481 atcttttaga agaaaaaaag caagagtggc tgacgacgaa agacgtagtc gatcaatttt
     9541 tggagggaaa ttaaatggat acaatccgta attatttaga cagtcttttt atcggagtac
     9601 ctcaaagtac cgagatagat aagctgaaaa ctgatttgtt ggcaaatatg gaagaccatt
     9661 atcatgaatt gatgggagaa ggaaaaaatg aacaggaggc catcggtaca gtaatttcga
     9721 catttggttc aatcgatgaa ttattagaag aactagatgt cgaaaaaaaa catcaagctg
     9781 acgagacgga aacaaacaca gcttctattt atttaagcga agcagaaaac tactggaaag
     9841 aatacagagc ggcaagtctc caagtggcat caggtgttct attcattagc ttatcttttg
     9901 caagttttct tttcttttgt tcagcagggt atgtgttcat gggtatttct tgtctgattt
     9961 tcggtatagc attagctgtc ggttttttta ttgcttctgg tatgaaaatc acacgtttaa
    10021 atcacttttt gcaccatcgg aaaatacctg aaaaagtatt agcagaagca aaagaaaaag
    10081 aagaagagta tcagcgaagt tttggcttct cactgattgc aggaattgga ttatgtattt
    10141 tttcactttt cccacttttg gcttctttaa tgtggtatat ggatggtagt atcggtgcct
    10201 ctatattttt tgttacggta ggaacgggag tcttcttgat catttatggc agtctggtcc
    10261 gtcattctta tcgtcaattc actcaaagtg cttattactg gtgaatgtat tcagatttgt
    10321 aaaaatattt tatttattgc ttacccatgt aaaatcgtta tagacagatt tcagttgact
    10381 tctgcgccat tgtacaggga taaaaagacg catactatct tcattgaatc cgactaataa
    10441 tttctttccg tcagtaatga tgggacgttg taacaaagta aaatgcgaga cgatcagatc
    10501 gacaagctct gaggtcgtac attggtcgat ttgatctttc aaatttttat aatcaatcga
    10561 gcgtttagct aatagatcgt caaatccgtt ttctgttaat gacaataaat aaaatatctc
    10621 tttccttgtt aaaggctgtt taggtgtgaa aattcgttct ctgtaaggca tttgatggtc
    10681 tttcagccag tttgccatca gatggtaact atgtgtgcga gaagaataga tcgtaagcat
    10741 aaaaaaactc cttaaatagt ccattaattt gttagatctg ttgagataaa taataggtaa
    10801 ggagctgtgc ataagcattg atcaaaacaa cccaaacatc attagaaata actttctaaa
    10861 gtcttaaact tgcgacgaga aacaactaga ctaatagaaa aaatgtctta gccatttggc
    10921 ttcctgtttg tttttttgct tatgctgcag ctcctcggga aagggttcta acaatttgaa
    10981 gaaggttgat gattgatcta gtttaaattg tgtgaaattt gaaatctggt gtttttacta
    11041 gatgaatcac ctaacaacta tgattataac ttatagaact tctgttttgt acttggtttt
    11101 gttatagttg ttataaagtt ttattttatt acagaacatt tttaatt
//
LOCUS       DAZUMO010000084        11003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000084 DAZUMO010000000
VERSION     DAZUMO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.084"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..2877
                     /locus_tag="ACG8ER_002396"
     CDS             88..2877
                     /locus_tag="ACG8ER_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS5050930.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2924..3451
                     /locus_tag="ACG8ER_002397"
     CDS             2924..3451
                     /locus_tag="ACG8ER_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS5050931.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3472..4662
                     /locus_tag="ACG8ER_002398"
     CDS             3472..4662
                     /locus_tag="ACG8ER_002398"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5050932.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4702..6000
                     /gene="asnS"
                     /locus_tag="ACG8ER_002399"
     CDS             4702..6000
                     /gene="asnS"
                     /locus_tag="ACG8ER_002399"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS5050933.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            6930..7604
                     /locus_tag="ACG8ER_002400"
     CDS             6930..7604
                     /locus_tag="ACG8ER_002400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5050934.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLIAQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            7601..7942
                     /locus_tag="ACG8ER_002401"
     CDS             7601..7942
                     /locus_tag="ACG8ER_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050935.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSFFAQF"
     gene            7972..8331
                     /locus_tag="ACG8ER_002402"
     CDS             7972..8331
                     /locus_tag="ACG8ER_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS5050936.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGISLIVNIIWLIISGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            8856..10937
                     /locus_tag="ACG8ER_002403"
     CDS             8856..10937
                     /locus_tag="ACG8ER_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5050937.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         3662 a         1937 c         2406 g         2998 t
ORIGIN      
        1 tttctttaaa aagcgatgag agtatttttc tgcactttgt agtataattt caaaagaaca
       61 ttagaggcag gagaagagga agatattttg aaaccaagaa cttatgcagt cgttgatatc
      121 gaaacgactg ggacgaatcc aaaagaagat cggatcatcc aatttggctg cgtcatgatt
      181 gaatcaggtc ggatcacttc acgtttttcg atcgatattc atccagggcg gaagatttca
      241 aaacaaatcc agcatttaac agggatcacg aatcaacgcg tccaaaaagc gccttatttc
      301 gaagatgtcg cccaaacgat ttacaatctt ttggcagaca ccatatttgt cgcgcataat
      361 atttattttg attataattt tttgaaccat gaactcatgc gatgcggact tcctagtttg
      421 aagataccag gcatcgatac agtagaactt gcacaaattt tcttgccgac ggaaccaagc
      481 tttcgcttag ctgatttgtc agaaagcctt ggatttattc atgagaatcc tcatcaggca
      541 gatagtgatg ccgaagtgac aggtcaactt cttttgctga tcgaagaacg gatgagaaaa
      601 ttgccaatca tcacgttaga gcagattgcg cgattaagta gacacacagg tatggatacg
      661 agtcgtttca tttatcatgt gattgaagaa atgaaagaaa aaccagagcc acttgatccc
      721 tcattagaaa tcgtcgatgg gctagctttg cagaaaaaag aagtggaact attcacatcc
      781 gtccattatg gtgagcggac atatccaaga aaaaaacaag caaaagaaaa aatgtttggt
      841 aaaacactga tgtatcgaaa agaacagaac agactgatga atgcggtcta cgaccatttc
      901 acaaaagatg aatcaaaaga tttgatgatc gaagcagcta caggaatggg taaaacaatc
      961 ggttatctgc tgccattgag ttatttggct acacctgaaa aacccgctgt catcagtacc
     1021 gtttcgctag ttttgcagca gcagatcata gaaaaagaca ttccattgct gaatcaattg
     1081 ctagatcagc ccatacaacc tgtaattatc aaaagctatc gccactatat tgatttacag
     1141 cgttttaaag gcacacttgt ggaaccgcca gaacaaaaac aatatgcttt gtatcaaatg
     1201 gcggtcttag tttggctgac gcaaacgaag accggtgatt tggatgaatt acatttaaca
     1261 aatttgaacc attcattctt tgcagatatc gcgcacagag gtattggatt tttagcaaga
     1321 aatcagtcat tttacgaaca agactttgtt cgtttcttgc aaaagaaaat caggcaaagt
     1381 aattttttga ttgtcaatca tgcgttttta atacaagaaa cacagcgaaa agaaccattg
     1441 cttccagcta gtccattttt gttgatcgat gaagtacatc atttgccgga aatcgctgaa
     1501 aaagtaaatg acaaacgatt aagtattaac tatttccata aacaaatcca gcaattagaa
     1561 gaaccaaatc agctgtttga ccgaatacaa agctatttac cgaaagaaca tgaagcctta
     1621 cgctggttat cgatttatcg agaagaactt tctgcgctat gcgaagcaca gatttgtatg
     1681 gcagaaggat taatgagttt agtgagcggc aagcaaccta acggacagta tccagaagaa
     1741 gtcatgatga caaaagagat gttggatcag ctgccactgg aatctgggac agccattgat
     1801 cacctgcagc ttttttatca ggagatccag cagctacagt cacaaatgcg tctgactttt
     1861 gagattatcc aagaaagctg gacaaaccag atgcggatcg atttagcaag tttatatagc
     1921 ttatttgatc aattagacaa gcagtacttg ctgatggatc aatggctgga agactggcga
     1981 gatcatttga tccattggtt cgttcctggg aacacagcgc agcagatgat ttttcatctc
     2041 catgattttc aagcggctat gttgccgtcg actgtttggt atcctcgata tgatcgtatt
     2101 ctttatattg gaggaacgct gaagatcggc cctaatcgac actatctctc taagcggctt
     2161 ggcattgaag cagcacctct taaagtgatt gcctctcctt atgattatga gaaacaggca
     2221 aggatctatg tgccaaaaga ggcacctgcc gtccatcaag tcagcaatca agcatatatc
     2281 gattatttag agacagcact tacaggtctg atcgaacaag aagatcggcc aatacttgtg
     2341 ttattcactt ctcatgatgt tttgcagaag gtctatcaga aaatgcatct gtctatgcta
     2401 gaaaaaggac gagaaatact agctcaaggg tttggcggaa gccgggagaa gcttttgaaa
     2461 cgcttcattt tgtcagaaaa aagcgtattg tttggcgctg acagcttttg ggaaggtgta
     2521 gatttgcctg gagatgcttt gcagatcgtt gtagtcacac gtttaccctt cgataatccg
     2581 cttcgcccga tggtcaaagc taggaacgct tatttagaat cagaggggat caatcctttt
     2641 taccaggaat ccgtacctaa agcagctttg aagttacgcc aagcattagg tcgcttgata
     2701 aggacggaga cagatcgtgg cgtattgctt attttggatc gaagactagt cactgcaaaa
     2761 tatggtgcac ggatcatcca tgcattaccg aaaaaagtac cgatcaaaga gctagctatg
     2821 gaggaaatct atcaggacat ccatgaattc ttaaagaaaa atgaagaagg aagttagagg
     2881 agctgtcttt tttcataatt aaaatctgct ataatgaaaa ggaatgaaga taaaggagcg
     2941 acgacccttg aagaaaaaac agacaaagga aaaaacgatt aatcgcgtgt tacttggctt
     3001 gatcgtattt ttattagcgg tcatcgtgat cagctcgatt ttttacttgc gttccagtcg
     3061 tccggcggtg caggcgaaga aagaagcagt agaaatcgca aagaaatatg caaatcttga
     3121 ggaagccgat catttttatt ggtttactcg aaagaagaca tacttttcac tgacaggtac
     3181 aaatgataaa ggaaaagaaa tcgccgttat cattcctaaa tctggtgaaa aagtaaaagt
     3241 attggatcaa acaaagggac tgactgaaaa cgaagcaaaa cagacaattg ctgcggccca
     3301 tcctgaaatc caaatcgaaa aagctgcttt agggatgtat gatgatcagc ctgtgtggga
     3361 agtagtagga aaagaaacaa atgggaaact gaattattac ctgattggct ttgaatctgg
     3421 agatgaagtc aagacaatca agggaattta aaaggtaaag gaatgaatgg gatggagtta
     3481 tcaaaaagaa caaaacgatt agaaccgtct gtcactttgg cagctgcggc aaaggccaag
     3541 gcattaaaag caaaaggcag agatgtgctg agtttgactg tgggagaacc ggattttgcg
     3601 acaccagaaa acatccaaga agcagcaatc gaagcaatcc gcaatgggaa agctagctat
     3661 tatacaccaa cagcaggaat cccagagctt cgacaggcaa tcgttgatta tctgaagaaa
     3721 aactatggac ttgtctatga accttcacaa acagtcgtaa cagacggagc gaaatttgct
     3781 ttgtataccc ttttccaaac gatccttgac ccgcaagatg aagtcatcat tcctgtgcct
     3841 tactgggtca gttacggtga gcaagtaaaa ctggcagaag gacttcctgt ctttgtgaaa
     3901 ggggaagaat ccaatagctg gaaagttact gttgagcagc tagaacaagc acgttcagaa
     3961 aaaacaaaag ccgtaatcat caattctcca tcgaatccga ctggaatgat ttatagcaag
     4021 aatgaactgc aggcaatcgg tgaatgggca gtaaaaaatg atattttgat agttgctgat
     4081 gatatttatg gtcgtttagt ttataatggc aatgagttta ctccaattgc aacgatttca
     4141 gaagcaatca aaaatcagac catcatcatc aacggcgtgt ctaaaacata tgcgatgaca
     4201 ggctggcgca ttggttatgc ggtggggaat tctgagatca tcaatggaat gatcgctgtt
     4261 gcttcccagt caaccagtaa tccgacagca gtcagtcaat atgcagctgt tgaagcattg
     4321 aaaggcgagc aagatacagt ggaagaaatg cggaacgcct ttgaagaacg cctgaatacg
     4381 ttatatccgc ttgtggcaga actgccgggt gtttctcttg aaaagccgca aggagctttt
     4441 tatctttttc ctaatgtaaa agaaacgcta cgcatgtgta aatatgaaaa tgtcacacaa
     4501 tgggtagaag atcttttaga agaaacaggg gtagcgcttg tgactggcga aggctttggg
     4561 gcaccagaaa atgtccgcat gagttatgct actgatcgcg ctacgttaga agaagcggta
     4621 cgcagaatca agcaatttat tgagagtaaa agtcaaaatt aatcctgaag aaattcaagg
     4681 aatataaaag gagagtcata cgtggaacaa attcaaatca tcgattcgaa aaaccatgtg
     4741 ggagaaacag taacaatcgg cgcttgggtc gcaaataagc gatcaagcgg aaaaatcgca
     4801 tttttacaat taagagacgg aacagccttt ttccaaggtg tagttgtaaa aagcgaagtg
     4861 ccggaagaag ttttccaaac agctaaaaat ctacaccaag aaacatccgt gttagtaact
     4921 ggagaaatcc gtgaagacag tcgttcaaaa tttggttatg aaatcggaat tacaaatgtg
     4981 gaagtggtaa gcgagagcca cgattatccg atcacaccaa aagaacacgg aacagacttc
     5041 ttgatggatc atcgtcattt atggctgcgt tcttcaagac aacatgcgat tatgcagatc
     5101 agaaatgaaa tcattcgtgc aacgtatgaa ttctttaatc aacatcattt tgtcaaaatc
     5161 gatccgccga tcttgactgg ttctgcacca gaaggcacta cggatctatt cgaaacaaat
     5221 tactttgatc aaaaagctta tttatctcaa agtggccaat tatacatgga agctgctgct
     5281 atggctttcg gaaaagtctt ttcattcggt ccgactttcc gtgctgaaaa atcaaaaaca
     5341 cgacgtcatt tgatcgaatt ctggatgatc gaaccagaaa tggctttcac tcatcaagaa
     5401 gaaagtttag aagtacagga acaatatgta gcattcttag tacaaaatgt tttagatcat
     5461 tgcgaatacg cattagatgt actaggacga gacaaagaag tcttaaaaac ttatacaaaa
     5521 ttgccattcc ctcgtattac ttacgatgat gcaatcgaat tactgaagaa aaatggattt
     5581 gatgacatcg aatggggcga agactttggt tcacctcatg aaacgttcat tgcgaattca
     5641 ttcgaacagc ctgtctttat cctaaactat ccaaaagcca tcaagccatt ctacatgaaa
     5701 ccacatccaa caagagaaga cgtggtgatc tgtgcagata tgatcgcacc agaaggttac
     5761 ggtgaaatta ttggcggaag cgaacgggca acagactatg atttcttgct agaacaaatc
     5821 cgtaatcacg gtttggatga aaaagaatat tcatggtatc ttgatctacg taaatacggt
     5881 tctgttccgc actctggttt tggtctaggc cttgaacgaa cagtcacttg gattgctggt
     5941 atcgaccatg tgcgtgaagc tattccattc ccacgtttgt tgaaccgaat ttatccataa
     6001 gaggttgtga aatcagtgcc ttgacctgag tattagagaa acaaaacgga aaaatagctt
     6061 ctcatatttt accgtttttt tagctaatat cgagggtcag ctgattgaac accgtttata
     6121 tcaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
     6181 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt
     6241 tattctaaaa aagaactgcg aactctcgca gttctttttt ttaaatacgg gataaaggaa
     6301 gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat
     6361 aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctaattt
     6421 gatgatgctt ctgctgttga aataaagcat catctagcac ctgaaaacaa actaatagta
     6481 acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa
     6541 ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag
     6601 aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt
     6661 ttgatggcgt catcagtggt ctgattgacg gaagcttggg tatgatcgat ctctctggac
     6721 caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc ataaaaaact
     6781 agacacttga tgagcgttca ttcaattgtt tgaagggact aggctgatgt cattttttat
     6841 acatatattt tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat
     6901 cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata
     6961 gcattacaga cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac
     7021 ttttgattgc cggaaaacta atcgcccaat atccaacgat ggaatttgaa tgcatgaacc
     7081 gtgggatagg aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag
     7141 ctttacaacc tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta
     7201 gcgatcaggc tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa
     7261 aattactaaa tgaacttcaa acagcggaaa tctgcaacat catcttgatg gagccgtttg
     7321 tacttcctat aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa
     7381 ttgttcgtaa acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca
     7441 atgctttagg gattgcttat ggtttttcga aatatacaag agaagatggg gtccacccaa
     7501 cgattggtgg acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt
     7561 tttttagtgg agataaaaat gagcgaaaga aaagtgaaaa atgatggaaa taaggtgggc
     7621 agtaaaagaa gatttacctc aaatcaatca gctgattact gatcatccag aatcattcgt
     7681 aaaagcaaaa acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac
     7741 aaaagaaaaa cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt
     7801 ggatcagcga ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga
     7861 agaaatccaa aaaaaatttg cagaatattt gatgctggaa gctaaaaaaa gaagaataaa
     7921 atcatttttt gcccagtttt aatcaaaaac agtaatagaa ggagaaatat aatgagttgt
     7981 ttaggaaatg tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca
     8041 gcggggatac tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag
     8101 atcgcctccc tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcata
     8161 tcattgatcg ttaatatcat ctggctgatc atttcaggcc taccaattgc tctcgcacat
     8221 gtcgtcagcg gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct
     8281 ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaaagagaat
     8341 aataaaggtt agaaaacgca tagagtcagg tgggatacat gtatttcacc tgactctatg
     8401 tgttttcctg tataagttct cccatgtgac ttttacgtct tttatttata tgggattact
     8461 gtttatggaa agttgaaata attttcatta aagaactctt gctatatttt tgaaagctta
     8521 gtggagcttc gaatatctgg gaaggacatt ttgcagggat tgaaaaaaga tattgtccta
     8581 tcgaaaaatt aatgaattta gacaaattgc cagcaacagg cttcaaactt agttgctatc
     8641 ctgtcaagat caagaaggca agtgcgggtt ggataagagc agtggcaatg atcgaaaaga
     8701 aaaagaaaga ataagccaaa acatctgtat ggaagaaatt ttccgatgca gatgtttttc
     8761 attttttctt tcggatacct tgacatatga ataaccgttc atttataatt agttatgaag
     8821 agatattcat atgttgaggc gaggaggtca ttaaaatgaa aagttacaga ttagaaggtt
     8881 tagattgtgc gaactgtgca atgaaaatcg aaaaaggagt tcagaagatc aatggagtca
     8941 aggaagcaac ggtaaacttc acttctggaa aactaacgat tgacgcagag gaagatcatt
     9001 tggctacgat tgaacaagaa ataaaaaaag tagtgaagga attagaacca gatgtcaaag
     9061 taacagaaat agataaggaa aaagtatctg aacatggaaa tgaaaaagag agaaatacgc
     9121 tgttccggat tttattttcc cttgcaggta tcgctttatt gctattattt gattttaatg
     9181 aaccaattcg cttgataggg tatttattga tttatctgct gataggatat gatgtagtga
     9241 agaaggcagt gatgaatatc gtcaaaggaa aaatctttga tgagaatttt cttatgtctg
     9301 ttgctacgat tggcgctatg ttgatcggtg aatatccaga agccgtagca gtgatgctat
     9361 tttatcaaat cggcgagtat ttccaaggat tggcagtcag tcattcaaga aaatcgattc
     9421 gcgaattgat ggcgatccgt ccagaaacag ctcacgtgca gacgacggaa ggattgatga
     9481 ccgtgaaccc tgaagacgtg cagatcggtc agtttgtact tgtgaagcct ggtgaacgtg
     9541 taccgttaga tggaacgatc atcgaaggag aatcattagt cgatacgtca gcacttacag
     9601 gagaatcagt ccctaaatct gtatatgttg gtgaaacagt attaagtgga tttatcaata
     9661 aaaataaacc atttttagtt caagtagaaa aatcttatga aaattctact atcagcaaat
     9721 tattagaatt agtagaaaat gccagcagta aaaaagcacc agctgaaaat ttcattacta
     9781 aattcgcgcg ctattacaca ccagtcgttg taggattggc cgttttatta gctgttttac
     9841 ctccacttgt tgtttctgga gcggcgtttt ctgaatggat ctatcgagca ttgacatttt
     9901 tagtgatctc atgtccatgt gcattagtga tctccgtacc ccttagtttc tttggcggta
     9961 tcggaggagc aagtaaaatc ggtgtattag taaaaggttc aaattacttg gagttgcttg
    10021 cacaaactga gacagtggta ttcgataaaa ccggcacatt aacgaaaggt gacttttcga
    10081 tccagacgat cgatacaaaa atcgatccaa agatattcat gcaatatgtt gcgagtgcag
    10141 aacagttttc tacacatccg attgctcaat ctgttttaga aggctacgca ggtccgcttt
    10201 taccaacagc taatatccaa gaatttgcag gtgaagggat cttagctgaa gttgatggca
    10261 aacaagtatt agcaggtaat cacaaattga tggaaagatt tgaaatctct tttccttcaa
    10321 gtcaagaaat cggtacattg ctttatctag cgatcgatca aagttacagt ggttatcttg
    10381 ttattgcaga taccttgaaa gaagatgccg tagacgcatt ggttcaatta aaaaaggctg
    10441 gtgtgaagaa cacagtcatg ttgacgggcg attcgaaaaa aattgctgat catatcggga
    10501 agcaagtagg cgtggacaag atatatagcg aattgcttcc agaagataaa gtgcagcggt
    10561 tagaggaaat cttacaaaga aacaataaaa agaccgcatt tgtcggagat ggaatcaacg
    10621 atgcaccggt gctagcaaga gcagatgtag gaatcgcaat gggtggattg ggaagcgatg
    10681 ctgcaattga agcagcagat gtagtcatca tgaatgatca accatcgaag attgcagaag
    10741 caatccattt agcaaagaaa acattgaaaa tcgtcaaaca gaacattgtc tttgctattg
    10801 ggataaaaat tttggtactt gccttaggtg cttttggttt tgcatcaatg ggagatgcag
    10861 tatttgcaga tgttggggtg acagtattag ctgttttgaa tgccatgcga agcttgcatg
    10921 taaaaaataa acgttgatca atgaagagga cgaatgaacg tcctcttttt tttatatcct
    10981 tttcttaatg aaaaatgtaa aga
//
LOCUS       DAZUMO010000085        10701 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000085 DAZUMO010000000
VERSION     DAZUMO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.085"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..487
                     /locus_tag="ACG8ER_002404"
     CDS             50..487
                     /locus_tag="ACG8ER_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050938.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            582..1466
                     /locus_tag="ACG8ER_002405"
     CDS             582..1466
                     /locus_tag="ACG8ER_002405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5050939.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1690..4857
                     /locus_tag="ACG8ER_002406"
     CDS             1690..4857
                     /locus_tag="ACG8ER_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS5050940.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4925..5422
                     /locus_tag="ACG8ER_002407"
     CDS             4925..5422
                     /locus_tag="ACG8ER_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS5050941.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5441..5905
                     /locus_tag="ACG8ER_002408"
                     /pseudo
     CDS             5441..5905
                     /locus_tag="ACG8ER_002408"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5902..6660
                     /locus_tag="ACG8ER_002409"
     CDS             5902..6660
                     /locus_tag="ACG8ER_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5050942.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6746..7486)
                     /locus_tag="ACG8ER_002410"
     CDS             complement(6746..7486)
                     /locus_tag="ACG8ER_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5050943.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7547..8800
                     /locus_tag="ACG8ER_002411"
     CDS             7547..8800
                     /locus_tag="ACG8ER_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5050944.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9122..9349
                     /locus_tag="ACG8ER_002412"
     CDS             9122..9349
                     /locus_tag="ACG8ER_002412"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5050945.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9472..9798
                     /locus_tag="ACG8ER_002413"
     CDS             9472..9798
                     /locus_tag="ACG8ER_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5050946.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9866..10579
                     /locus_tag="ACG8ER_002414"
     CDS             9866..10579
                     /locus_tag="ACG8ER_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5050947.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         3651 a         1804 c         2240 g         3006 t
ORIGIN      
        1 atcttaaata aagaaaacaa tatcaaaaaa tgcacgtaga agtagataaa tggagtggtt
       61 ttgcaaattt cttttatttc tagaagttat tcacgtatac ggatttgttt ttctcacaaa
      121 taaaaaggca ggaagtacaa tggatcattt gtatataaaa tatattcatc aattttccgt
      181 tttggtgtta ggcacactaa ttatagcggg gattgtcgca tttattattc aaggccgtga
      241 catattaaca gtacatcaag agtttttcag ttatgcattt ggtattcctc tgatcgctat
      301 aaggtctgat aacaaaaagt ggaagaaacg ctattacatt ctcttgctat ttttgtgttt
      361 ttatctgcca cctaagattt ttactttatt aggtatacaa tgcctatcat tttcttttat
      421 ttcctatatc attgttttaa ttttcctcag tttcatagta tggatgtttt atcttgtcaa
      481 aaaataaatg aatgtttcat gaacagtact tcatcacagc ctaatttttt tctttatgct
      541 atcgttatat taaagaaatc aatcagaagg gaaagaaagt gatgaaaaaa gttttattag
      601 tggtaaaccc cagttcaggc ggtgaacaag caaaggaatt tgagcaatta gcgatagcaa
      661 aattggaatc ggtgtttgat gaagtggtcg tcttgcatac aaaaaaagca ggggatgcaa
      721 aaaactttac tcgcgaagcg gctacggaag ggtatcatag tgtgtttgtc atgggcggag
      781 atggaacagt caacgaagga atcagtggaa tcgctgaaca agaacatcgg ccaaatttcg
      841 gatttttccc attgggtacg gtgaatgact tggctcgagc attagggata ccgctagaac
      901 ctgaggaagc gatcaatcat ttttcgattg aatcagtcaa gccattggat attggaaaaa
      961 taaatgacga ctattttatg aatgtcgtcg caattggctc tatccctgaa tcaatcaatg
     1021 atgtagatgc ggagaagaag acgaaatttg gaaaactagc ttatttcatg tcaggaatca
     1081 aacagttagc ttccacacaa tcctactcgt ttcacgtaga agttgatgga aagaaggaag
     1141 aaattgagag cagtaccttg ctgataggac taactaactc agtcggcggt tttgaaacat
     1201 tacttccaaa tgcaaaagtg gatgatggta agctccattt ggtttattta aaagattctt
     1261 ctttgatcga tacgcttaaa acattgccag acctattaaa aggagtagat gaatctacga
     1321 aaaatctagc ctaccttact tgtgagcaat tagttgtgtc attagctgat gaggaagaat
     1381 tgacgacgaa tgttgatgga gatgaagggg ataaattacc tgtgacaatc aaagtattgc
     1441 cctcacattt acaggtttat tgttaaaatg gaggttgtga ccgaggagtt ggcgggagaa
     1501 caccgcttgt tcgggataag ggactgtgac aagacttttg tcacagcctt ttttttgtac
     1561 aaaaaagaaa tttacaccta atagaaaaat ttttccataa aaatacatat tttaaatatg
     1621 aaagtacaat tggaaataga ttaaaacgat aaaataaaat tgtcaaaacg tcagagagga
     1681 tggtggagaa tgaatcaatg tgtaaaaaag ctgattgcgt cgattttgtt tgtcttccct
     1741 ctcttgatgg tgacatccgt ttttgccgat gtagagaaaa caaacgaatc aacgcaatca
     1801 gagaatcaaa gttttgacaa gactactttt tcagaagaat cgattacttc tgaaactaca
     1861 gaatctcaaa gtgaggcttc tactaataaa aggcaagaag aaaataagac aagtgagacc
     1921 tcagaagtag ctgaacaaac gtctgatagc agtgaagcgg cactggcaag agctgtttca
     1981 gcggatgggt acagcgaagc tgccacaaca gaagaactgg cgcagcttct cgcagacgaa
     2041 tcagtaacta agatccgact gattcagcct ttgactttag acagggaatt agagatcaaa
     2101 cgagatatcg ttattgattt tggtggattc gctcacaatt tcggcacaca ccatatttat
     2161 atcaatgaga tacctcagtc gatcgagttc cagaatttta aaggaacagc tgcacatcca
     2221 ggtggattga caccaaatat cgatggaaat gccattatca ttgcttatat gaccgatttt
     2281 tggggaagca gatatcattt tacgggagaa ataaaattta ccgggacact tgatttgtat
     2341 gatgggtcca aattaggact gatttacgct cctcgtgcaa ctgtcacatt agatggtgtt
     2401 tccggtgtgt tggatgtcca accagtgaag gaaggactga atgccgctcc gggtaaagcc
     2461 tatttttgta gaagctatca attgaatgtg atcaatggta gtcaacttta tggaccgtat
     2521 ctagggaaat tctacggttt tctagattca ggagatgaga ctggtagtgg aaaaacagcg
     2581 ccaggaatcc atatcttaag cggttcaaag gtctctcttg attatgatcg ggcagatggc
     2641 gttagcgatg aaggagaggc agtcgatacg ctacctggta atgtcatgtt tgaagtcagt
     2701 ggttcgggaa gcgaattttc ggtaaataca aagatcaata ttaccaatga caacaatcgc
     2761 ggaatcatcc aaatgagagg ttcaggatcg aaagtcactg tatcgaatgg tgggaaaatc
     2821 accatcaata cagcaactac gggcggattt cgcttgcaag gagattctag tcaaatccat
     2881 gtatcaagtg gtggacaaat ctcggtcaaa atggctggag atgacgatca acccgggaac
     2941 aatgggatgc gttttgtagg aaaaggcctt tctttgatcg tagaaggagc cggatcagtt
     3001 atcgacatcg ataagcagag tgggcgttcc agcgcagttc gttttgagaa tggtacgcaa
     3061 aagctgacag taaccaatgg aggctcattg agggtaagaa atacaggtga tggcaaatct
     3121 tacgtgaaca gactcaatca aggaaatcaa gcgatccaat tttatgacgc ttctggtttt
     3181 ctagaaagtc caggttcagc tgattttaca gtcactggag aaaaatcaaa tatcgatata
     3241 cgcgcagatt ccggagcaac tgtagataca actggagata ttgatttaaa ttttacggca
     3301 ggtgaaaaaa cttatttagt catggtcggg aaaacagggg aagataagac cggcatcatc
     3361 agtggggatg tgttgaatgt ggacttagaa tctcctacct attttgattt ccaaaacaaa
     3421 cggattggaa gcgagaccaa tggcataggc ggctgggtat tccaaggaaa tggagattct
     3481 tctttaaaga tcgcccattc agaaatcgct ttatggcgaa aaacaggcgc aacattcgat
     3541 aactttgaaa aagacccgga ttatttttct gatatgacag acttatcgat caatgggacc
     3601 gacttaggca atttcgtatc aagccctagt gcaggtttaa caaatgaatt tgctcagaca
     3661 ggtgccggaa tgaaaaatta taatcgaatc agtgccaata atcagctccc gatgattgaa
     3721 tccttaagag tgccaacgaa tgcagataaa aaaatctatg ggcatgtcgt tgttcccgaa
     3781 ggtgtcgagg caaagccaag agatgcttgg acaggcgaag tagaagtgac tttacgtatt
     3841 acttacgcag atacgacgaa atcatctgta gaaattactg ctctcacgca aggacaaaca
     3901 gatgaaaatg aaggggcagg atataaccca tggggagaag gcgaacaagg cggcttgttc
     3961 gaagttcaag tacccaatga tgagagatta aaaacggagg atcaagtgca agttgttgct
     4021 gctaaaaaag taagaggtgg ccaagaagtg acagaactgg actctgccaa aacgacagtc
     4081 gatgtgattc ctcctgaacc tgctaattta agtaccacat tgatcaatgc agcttctcgc
     4141 acgatcacag gtacaggtga agcaggagcc ttggtttcat tgaaaaaaaa tcaagctgtt
     4201 ttgtcagaaa caactattga tgatacaggg gaatttgttc tttctatccc agaaaatcaa
     4261 ctttttgcag gagatgtatt agaaattgta ttaaacgacc aagcaggaga agctactgct
     4321 aagggtgtgc agaacaagcc aagtaccaac gatgaaattg ggaatcacaa tccaagtgat
     4381 acgccagttg tttatcatga tgccccgcct tttgcacctg ctacgaagat cgtagtagaa
     4441 ggtggtctct catttcttgc cccttccaaa ctcgattttg gtcagatcaa agcaactggt
     4501 ttgacacaag aagcatttgg ccaaatggat atacaggaaa aattatggat ttatgatcag
     4561 cgagaaacga aaaatccgtg gatcttaacg ataaaattat ctcagccttt cacaaatgat
     4621 gcaggatttt ctatgcagga tgtcctatat ttcaagaaag gagaggagta tcaattagtg
     4681 aatgaagaag aactggaaat tgtcagagga caaaattcaa cgaatgtgca agcagattat
     4741 tcagaatatt taaataacgg tcgggcgttt aaacttgtct taaccaaaga aaatcagata
     4801 gctggtaatt actcagcaga gataacatgg acattagcag atgcgccgac agaataaaga
     4861 tcaaggagga tgattgatga atcgaatatg gaataaaagt catcgaaaca actgctcttt
     4921 acttatgatc cttctccttg gattcatcct ttggcagaca cctgtccatg cctccgaatt
     4981 caattttgcg gtagattttg aattaccggc taatcagatt gataagaaca aaacatatat
     5041 aaatgtaagg atggagcctg ggaaaacaca ggaaatcccc tacacggtct ttaaccgaac
     5101 agataaatca gtaacagttg agacattgat ccactccgct acgacgaatt caaacggtgt
     5161 gatcgaatat ggagaatcaa aaagtaaatt tgatgaaaca ttaagttatg atatgaaaaa
     5221 cttagcaaaa acagtgggag aaataactgt tcctcctcaa agtgcggtca ctgagacact
     5281 ttcactgaaa gcgccagaaa aagagctggc tggtgtggtc gcaggaggga tcacgtttca
     5341 agaaaaaaaa gagaacgttt ctaacaaaag tacagttggt gtcgtcaatc agttttctca
     5401 agtgaaagca atcttgattt gaaatagcga aaaagagtct tccccagatt tgaaattaaa
     5461 aaaagcttat gcggatcaaa gcgatggaag aaatgtgatt aaggcgaatc ttcaaaatcc
     5521 aaatgcttct tatatgttcg gcttgactac atcggttgaa atctacaaaa aagaaacaaa
     5581 agagccttac ctttcaataa aaaaagaaaa aatagatgta gcacccaata caaattttga
     5641 tttacttgtc ccattagaag gaaaaaaatt agctccggga cattatcaag cggttgtgaa
     5701 gggtgcttgg aaagaaaaac gctggaatct ccataccgat tttgaaataa ctgatgatgc
     5761 agcggaccaa ttgaatgaaa aggatattga tttaaaaaat cagccagaaa catttgactg
     5821 gcgatggctg atacttgttg gagtcattct tattggctta tttgctggat tatttatttt
     5881 aaaagaaaga aggaaaaaga aatgaaaaaa acaattgcat taagtaccgt tctagctgga
     5941 agtctattat tatgtacaca aagtgttttt gctgagaata agagctcagc cactacagaa
     6001 ggaacgattt ctgtgaccaa accagatgct gcaaatcctg aagacgttgt tccagttcct
     6061 cctgttgatc caaatgatcc tgatactcct tttacacctg acgaaccaaa tcagggaaca
     6121 tccggattac tgacgattga ccaagctcca gatcttgatt ttggaacaat caagttaggt
     6181 acagcaaaaa cagtatatgc tgcattacaa agcggaacgg acagtactgg agcacaaaca
     6241 caagtacaga atttcgtaca agtaacggat aaaagcggaa attatgccgg ttggaaatta
     6301 tcagtcaaac gaacagaatt tattggtaca acatctgata cgaataaact aactggttca
     6361 aaggtgaaat tcaaaaacgc cgtgattcga aatagcgcaa ctaatagcag tggaatgcca
     6421 tctaccgttt ctggaactgg cgctgaagga atcgaaatcc cagcagatca ggcagtagat
     6481 ctagtaactg caggagcaaa tgaagggatt ggcacatgga tttattcatt aggagagaat
     6541 gtggaacaag ggagacaatc agtggaatta tttgttcctg tatccaatta tgctgtagac
     6601 aactacgttt caacattcac atggtcgtta acagatgctc ctacagaaga ggaagcgtaa
     6661 aatcatatca acgatttcag ggagtggagc cgattcaagg cccactccct gaaatctgct
     6721 atgcttacgt ctcagctctt attcatcaac tcgaagcagt tttttggaag tcatggaaac
     6781 gttcctttgt cttttcaaag atctttcgac caacgagtaa ggagtcaccg ttgaaaaatt
     6841 ctaacacata atgagcagaa tcaattttct gcaattgcga taagttgata cagtaagatt
     6901 ttaaaagtaa aaagtaagca tacttttcta actctgtctt caattgtttt aaagaagtat
     6961 tcactaattt atcttctgta gttgttttga ccataacttg gtttttcaaa cctttgatac
     7021 tttcaatata taaaatttcc cgaggataca ggacgagact tccacctttt tgatcaataa
     7081 agattttatc gacatttacg atctgctgtt ctttccaatt gatctgttct agcacttgac
     7141 gaatttgttc agataaattc ctgtcttttc tgatatatcc aatcggaagg attttccgat
     7201 tgatgatctc tactgcaaaa ttcgtataag cggtgaaaaa aacaatatta ctatgtgcat
     7261 tttttttacg tatttgttcg gcgacatcaa tacctgtgag tgattgcttc aagtcgatat
     7321 cgataaagaa aacatcgtga ttatcaatag tgcccaactg gatcaactga ttgatttgag
     7381 agatagaagt gagctgcacg atattctcga tatgatgatt gaccaattct ctgatgatcc
     7441 attcgctgtg aaactggtta tcttctatga taaaatactt tttcatatgc aggctcctat
     7501 taaaaaaata tgtgtacatt ttgaaacaaa acaatggagg aataagatgg tcgcaacagt
     7561 gacactttat ttgaccttta ctttgatttt agatttaggt atctatttat tcttttcgaa
     7621 acaactgcta gtaaaaaaca gctttggttg tatagctttg atggtggtga cacttttact
     7681 tcctatccct aaagtgagct ttccttacct agttggctta ttattgatgt ttctttttta
     7741 ttttactacg aaaaacctgt ttatcagctg tttgatcagt aatctttttt ttgctatcac
     7801 tggactcagc ttttttctgg tttttgatct gatggattat ttaggaaaga gacatcttgg
     7861 aacagcaagt ttttttgcac tacttatcgc atttattttc tatctattag taaaaagtat
     7921 agacaaacag acgagatttc tcaacttgct ttttacttat ccaactgaac actattttgg
     7981 aggtatttgt attttattaa gctggggcgt gatttacttg gtattgggaa gtgttcactt
     8041 ccgctcatct aattatctgc ttctatctct tttgtcgata gtcatcagtt ttttttcttt
     8101 gtttttttcc attatgttga tcagtttcca agtaaaagaa agacaacatc aggaatcgct
     8161 tcaactgtat caaataaatg aagaatatta ccatcatcta gaggaattca aacatgatta
     8221 taaaagtcta ctgttttcat taaaaattac tcttcaggaa aataaaaaag aaacgttgga
     8281 atttttagaa atattagaag atcattctgc agctattttt gaaaaaccgc aaatacaaca
     8341 gctaacaaat attgtatccc cagctgtcag gggtgtcttt ttaaaatttc tagaaaaagc
     8401 agaatcggag aaaatcccga tccaagtagt tattcctgtc gaagtgaaaa caatcccgat
     8461 tgatctggtt gtttttattc gctgtttatc tatttttatt tctaacgcat tcgatcatcg
     8521 tgtagccgat cagagtccga ttacgatcaa tctgctggaa gaaaaagagg gatcacgttt
     8581 tagtatagcg aacaaggcaa atccaacgaa tttgacgcta tcggagatgg tcaaaagagg
     8641 aaaaacctct aaaaaaaatg gcgggttagg tctatacagt gcgaaaaaaa tgttggatgt
     8701 ttacgaaaat gcagaactaa aaatcgaatt ttcaaaacag aatcactatt tttttgtaga
     8761 aatgtatctg gcatctagaa agagcaaatc agcctactga cttactgtca aaggataagt
     8821 ttgaggaaag tctacacgga gatagagaca acaacacagc atattcaaaa gaaacagaag
     8881 cgtgatgacc gttgtcaccc ttctgtttct tttgctatgt agttaaacaa agtagttgac
     8941 acttccgcta cttagtatta gtacgaaagt atacttcatg atactaagta gataagaagt
     9001 gaaaagcgga acccacgatc gtcaaaatgc taactgcttt gcttacacca gataaaggga
     9061 agagcaatat cgcttatttt aggcgtggag aagcaagcaa atccctacga tcacaaaaaa
     9121 aatgctgacc cagcagttaa gagagttaga acaagatgaa agtatccatc gaaaagccta
     9181 taaccaagtc ccgcctaaag tagagtactc gctcaccgaa gaaggaaaat cattaagaga
     9241 gattctagta gcgttgtctg tttgggaaga agaacgtatc gaaaaacaaa aagcctcaag
     9301 ggaagaagac acgctactgc atacacatgg tggctaccta aattattgat tttctatgac
     9361 atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat tttttatgta
     9421 aaaaaatggt atgattaatc aagtaagaag tgattggaag aaggcaagca aatgaatagt
     9481 atggaaaaag tcaaccagtt tagagatgaa cgtaattggc gacaatttca taacgaaaaa
     9541 gatttggcta tctctatttc tttggaagca tctgaattgt tagaactttt tcaatggaaa
     9601 acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact ggcagatgta
     9661 ttgatctatt catacatgat ggcagacaac cttcacctgg atctagatga gatcattgaa
     9721 gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg tgtacgaaaa
     9781 aaatacacag agttgtagaa atgtgtcatt cggctgttgg aaagagcaaa agaaaaacag
     9841 taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa gatggctagt
     9901 caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca atgaacagat
     9961 caaggattat tttgtcagag agacaggaga taaatatgct atagcgaatg taacaaaaga
    10021 agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg tagaaccaat
    10081 gacgacagaa ggggtcatga ggtcgcaaat gagaggaaca gatctaccag tgattgctag
    10141 tatcgcagtc ccttcagttt caatcaattt accggttttc aaaggcttgg ataatacgtc
    10201 actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag gaaattatgc
    10261 cttagcaagc catcgtgcaa cgaaccctga attgctgttt acaccgctag aaaatttaga
    10321 gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca aaacattttt
    10381 taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg gaaaagaaat
    10441 tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg tacaaggaga
    10501 actggaaagt gttacatcga taaaagatgc gacagatgat atgagatctg ctttcaatct
    10561 agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa aacagccaaa
    10621 ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agagaaacgc aaagaggtgc
    10681 atctctgatt tggttgtttt c
//
LOCUS       DAZUMO010000086        10619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000086 DAZUMO010000000
VERSION     DAZUMO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.086"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..2237
                     /locus_tag="ACG8ER_002415"
     CDS             291..2237
                     /locus_tag="ACG8ER_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5050948.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2240..2695
                     /locus_tag="ACG8ER_002416"
     CDS             2240..2695
                     /locus_tag="ACG8ER_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5050949.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2709..4037
                     /locus_tag="ACG8ER_002417"
     CDS             2709..4037
                     /locus_tag="ACG8ER_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5050950.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4071..4355
                     /locus_tag="ACG8ER_002418"
     CDS             4071..4355
                     /locus_tag="ACG8ER_002418"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5050951.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4357..4872
                     /locus_tag="ACG8ER_002419"
     CDS             4357..4872
                     /locus_tag="ACG8ER_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFS5050952.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4888..5550
                     /gene="rpe"
                     /locus_tag="ACG8ER_002420"
     CDS             4888..5550
                     /gene="rpe"
                     /locus_tag="ACG8ER_002420"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5050953.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5557..6129
                     /gene="hxlB"
                     /locus_tag="ACG8ER_002421"
     CDS             5557..6129
                     /gene="hxlB"
                     /locus_tag="ACG8ER_002421"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFS5050954.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6316..7836)
                     /locus_tag="ACG8ER_002422"
     CDS             complement(6316..7836)
                     /locus_tag="ACG8ER_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5050955.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8079..8264)
                     /gene="tnpB"
                     /locus_tag="ACG8ER_002423"
     CDS             complement(8079..8264)
                     /gene="tnpB"
                     /locus_tag="ACG8ER_002423"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5050956.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8248..8430)
                     /locus_tag="ACG8ER_002424"
     CDS             complement(8248..8430)
                     /locus_tag="ACG8ER_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050957.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8671..8889
                     /locus_tag="ACG8ER_002425"
                     /pseudo
     CDS             8671..8889
                     /locus_tag="ACG8ER_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9384..9983
                     /locus_tag="ACG8ER_002426"
     CDS             9384..9983
                     /locus_tag="ACG8ER_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5050958.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10047..>10619
                     /locus_tag="ACG8ER_002427"
     CDS             10047..>10619
                     /locus_tag="ACG8ER_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFS5050959.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3509 a         1670 c         1984 g         3456 t
ORIGIN      
        1 tattataata aaaaaatacg aattttagtg acgccattat attgttttta tttttcaatt
       61 gtaatatttc atattacttc cataataagg agtattttct attggaatat cttagctctt
      121 ttaaggattt ctcctttttt gtttctcatt ttttaagatt atttggattt cctttacaac
      181 aaacttccac aagtattatt gacatagatg taattgaggg aagggggttc cagtgctaga
      241 ataaataatg aaaagaggta acaaatgcta gcgttaagag gtgaacaagt ttgattgaaa
      301 gacatgtaaa tttattaaaa ttattaagtc gacaagaaga ctttaaaccc gcttcttttt
      361 ttagtgataa attggcagtg tcgaccaaga ccatttatca agatattgaa ttgttggaag
      421 attatgtagt taattaccaa gtaacaattg agcgattacc ttctttgggt atcaaactca
      481 taggtgatag gaaaaacgtt gaacatttga tttcatcggt agagaattat cttgaaaaag
      541 atatttattc tccagaaaaa agacgtgtcg agatcattaa agaagttttg tttggtgata
      601 agcctgtttc tttaactcat ttgtctcaaa gttttatggt tagtaaaaca tcgttataca
      661 atgacctgag aattatcaat aaaattattg gcagtggaga agttaagttg cagtcatctg
      721 cagaaggaat tagttttact ggtaatgaac aagcaattca acgagcaatc aagcaacttg
      781 tattctatta tgcgaatgac atggaatcaa aaacattttt tcaagtttta aatatgttgt
      841 ttgattcaaa agttattagt gtcattcatg atttgctatt caatgattac agtgaactga
      901 ctgaaaaggt ttctgattac tatgttcggt cattgatttc tactttagtt atccaatgta
      961 accgtttact gaaaggtcat catttaactg ttgaagaaga attcctcttc aatagcattc
     1021 gctatatgga aacgtacatt gtggcaaatg gtttaattga agaattgaag gcaaaattga
     1081 atcttccatt ttatgacgca gataaagaat atctatgtcg tcaactattt gcacatcgtg
     1141 tgacaaataa tttaaaaacc agcaatgaag agtacgctat gagcgttcat aaattgattg
     1201 aaagaatgag tgagattgaa aaaattgatt tgactcaaaa tgatcgatta tatcaatcct
     1261 tattgtatca tgtgccagca atggtccttc gtttaaaaaa aggtattaaa attcaaaatc
     1321 cgttattaga aaatatcaaa tctcaatatt cagaactctt tagcatagta tggtacgcca
     1381 tgtccatcct tgaagaagaa tttgatgtga ctttaacgga cgatgaagtt tccttaattt
     1441 tgattcattt tcagattgct attgaagatc agtccaaagc aaataatatc atcattattt
     1501 gccaatacgg tatttcttct gcgcaattaa tttacaataa agttcgacgg tttttaccag
     1561 caaaagataa tgtagaaata tcaactttag ataagtttca tttagctgaa attggggaag
     1621 ttgatttgat tatttcctct ctttcctttg atgcaggtgg tataccctta gtaagggtat
     1681 ctccattggt aagtaaaaca gattatatga atattatgga agcttatact aaatatttaa
     1741 tagaagaacc ttcgcgattt attcaaaatg aactgattcc taaagcggtt aaagttccca
     1801 ccatatctaa atttgttcaa tcatctttag taaaaacagg agtacaggtg gaaagcaaag
     1861 aagaatgttt ggatttgatg atttccgaac tagaagccaa ttcattagtt actgaagagt
     1921 ttcgtgcttc tatcttctct agagagaaaa tggggaatac taatttagaa agtggtgttg
     1981 ctttaccgca tgcgaatcca tcaatggtca ttgaatccaa catttttatt gtagcattaa
     2041 aaaatgcgat taattggggc gatcgaaaag tcaaattagt cattatggtt agtttagctg
     2101 aagaagatgt atctgagatt agaggagtat atgaagaact ttaccaattt attgggcaaa
     2161 aagaaatggt cgatcgcttg ttacaagttc atgatagcga aaaattacta agaatttttc
     2221 aacaaaaagg agaataaaaa tgtatctaga taaagaatta tgtttatttc aattagaagt
     2281 gaaagatcaa aaagaattgt ttcaagtaat gtctgaacaa ttaaaaaacg ctggttgcgt
     2341 gaaagatagt ttcttagaag gtatcaccaa ccgtgaacaa gaatttccaa ctggattaga
     2401 agtaaatcaa attgggtttg ctatcccaca tacagattcc gcacatgtaa atagctctca
     2461 aatctgtttt gcctcattaa aggaaccact ggtattttca gatatgacag ataaatctca
     2521 tgaaatacct gtacgtttag tctttatgtt ggcaatgtca caacctcatg aacaaattga
     2581 tacactacaa aacttagtta gcctatttca aaatgaagaa aaagtaaatg aattacttgc
     2641 atgtaccacg aaagaagcat ttattgagtt agtacattct gcaggagttt attaggagga
     2701 atagagagat ggatattgta ttaaatgcct ttgaatggtt tattggatta gggtcaaacg
     2761 tctttttacc actgattatt tttgtaatcg ggatattatt tggattaaaa cctggtaaag
     2821 catttgtttc cggcgttact gttggtatcg gttctattgg tttaggatta gttttaagtt
     2881 tattatcaga tggtttagga acagcaattc aacaaatggg ggaacaatac ggtggtggcc
     2941 tgaatatcct agatattggc gtaggtgttg gtggaccttt ggcttactct acatcattgg
     3001 gaattttaat gattccaatt tcattaattc ttaacttcat atttgtggtt ttgggttgga
     3061 caaaaacatt gaatgttgat atttggaact tttggttcgc aattttccta ggtatgatta
     3121 ctcaatctgt tacgggaaac ttttggtttg gcttaattgg cgcgattgtc gcagtaattt
     3181 tacaatggtt cttagcagat cgtacacaaa aaactgttag cgaatttttc ggctatccag
     3241 gaatctctat cacacatatg atgggattat caggagtact atttgccaaa ccagtaaatt
     3301 gggtttttga tcgtattcct ggatttaata aaatagatgc agatgcagaa acattaacaa
     3361 aacgtttcgg tgttttaggt gacacagttg ttattggcct aattatcggt attgtagtgg
     3421 gtattatggc aggctatgac gtagcaggaa ttggaaactt aggaattatg actgcggcag
     3481 tgatgaagat tatgcctaaa atggtagcaa tgtttatgga aggtttaatg ccaattgcag
     3541 aagcggcgaa agaatttacg aataaaaagt taaatggtcg ccaagtaaac attggaatgg
     3601 atgcagcgtt aactgtgggt catccaacta ttatgtcaac ttctctattg cttgtaccaa
     3661 tctcattatt gctagcacta attcttccag gaaataaagt attacctttt ggagatttag
     3721 cattctttgc tttcgccatc tgtttaatga ttccttattt taaaggaaat attgtgagat
     3781 cattagtagg atgttcgttg tatgttgtta caacattata tatggcaaca tggttggcac
     3841 caattatcac tgatgtgttt acattagcaa gtttcgatgt ggggacagca ggattagtaa
     3901 ctatgttgtt atgtggatta tggccagtag ggttgtttgt tttattagca tcttactttg
     3961 gtgtaattgg aataggtgga ttaggagttg ttttagtagg tgcttctatc tacatgaaca
     4021 aagtgaagaa agcttaatca taaaattaaa ataagaaaga agagatcaat atgaaaaaat
     4081 tattagttat ttgtggagca ggtcatgcta cttctacaat tgcagtagca aaattatcaa
     4141 aatggttgga agaagaagga ttaacagatc aagtgaaaat ttatcaatct aaaattgctg
     4201 atgagctaaa taaaattgat gaatatgatg ctgtagtaag tacaacaatt gtccctgatg
     4261 taattaaagc taaagtaacg aatggcttgg gcttattaac aggtattggt acagatgcca
     4321 tttatgcaga tgtaaaagaa agattaggtg tttaatatgc gttttcatca tgacactttg
     4381 actattgaaa cagtttcagg gcgtccttcg tatcatgata ttcgaaaaga acttttagat
     4441 acagttgata cagcaggtat tgagaatgga atgttagtaa tttcgagccc gcatacaact
     4501 tgctcactct tttttgaaga aacgatgcat gacacaaact tttttggtga tgattattta
     4561 catgtagaca tcaacaatgt aatggaaacg attattccaa aaatgacgag tgaaaatcaa
     4621 tacaacagtc cggggccaaa acacattgat tttggaacaa gattatctga tcccaattac
     4681 cctgcagaaa aatgggtcat gctaaatacc gatgcacatt tacgttcatc tatttatgga
     4741 accaactcaa tgactttaat tgttcaagaa ggtaaacttt tattaggaga aatgggccgt
     4801 gtctattttg tcgattggga tcaactaaga gagcgaacta gaacaatcaa tctcatgatt
     4861 atgggagaat aaacggggga atagagaatg acaattgctc catcattgtt aaatgcacct
     4921 atcgcagaag ttaaagagtt atttgaaatc ttaaaagaaa agaatgttga ttatttgcat
     4981 attgatgtaa tggatggctg ttttgtacca gatatggctt ttggaccaag tgttattaaa
     5041 gatattcgtc cactaacaga cgttgtgttt gatgttcatt tgatgattga tacaccagaa
     5101 aaaattgtca caagatttgt agaagcagga gcagatattg ttaccttcca cattgaagct
     5161 accaaacacg caatgaaggt cattcagtta gtcaaggaag caggagcaaa agtagggatt
     5221 acttttaatc ctgcaacacc agtcagtttt attgaaccag tattatcttt agtcgatcaa
     5281 gtattagtaa tgacaatcaa tccaggaaca gagaataagc attttattca agagacagta
     5341 gttaaaatcg aacagttaga tgtaatacgc aaacaaaatg attatacgta tgatattgaa
     5401 gtagatggca agattgataa tcaaacgatt aaagtttgtt ctaaagcagg ggcggatatt
     5461 tttgtatcag gcggtttcat cttcaatggg aaaaataccc cgcaagaaca aattgataaa
     5521 ctaaatcaat cattagagga agtgaagtaa aagatcatga ccaataaaga ccaaaataaa
     5581 aactatttag aagcgattat cttagaatta caaaataact caaaacgtat taattctgta
     5641 gagttagaac aatttagtga gaaaattgtt caagctaaac acatctttct agctggtgca
     5701 ggacgctctg gaattgccat gcaagcattt acgaatcgct taatgcattt aggtttttca
     5761 gtcagccttg tcggtgaaat tagttcacca cattcgcaac ctggagattt attaattatc
     5821 tgttctggct caggtgaaac aggtagttta aaaaatttag cggccaaagc aaatcaaagt
     5881 ggtgtaagca ttgctttagt taccatgaaa aaagactcgt cgattggtca attagcagat
     5941 tctgttcttg tattaccagg tattaccaaa gatgaaaatc aacgagaaga agatactttt
     6001 tcacaaccta tgggttcaag ttttgaacaa ttagcatttc tcacctttga tagtttagtg
     6061 ttatctttaa tggataaaac aggtgaaagc tcagaaacga tgtttgctcg acatgcagat
     6121 tttgagtagt agattttaaa gataaaaaac cattctctaa atgctaatta ttcagcaaat
     6181 atgagaatgg tttttttatt tgattagaca taacttaata attcagtagt tttgagcgta
     6241 agcgtgtcat agaatggtac ctaaacacaa atagcccgat ttgatacact caaatcaggt
     6301 tatttgtggc ttatattatt tacagatttc ttgtatctgc tgattccatg gcaaataagc
     6361 gcgtagcgct tcaggtgtca tcgaagacag attgggaatc tctgtaaaaa gatgatttaa
     6421 atatttgcgg atgtttaacc cattcgcaac agctgtctgg ataatactca atataattcc
     6481 atttgatcta gcgcctttta agcttttaga aaatagccag ttttttcgac cgatcactaa
     6541 ctctttgatg gcacgctctg ctcggttatt tgaaagttcc aagcgtccat caagcaacac
     6601 aatctgaagt ccgtctttat gattgactgc gtattgaacc gcctttccaa gctgcgattt
     6661 tggcaaataa gcaaaactct cacaccaatg aaaaaattca gtcatcacag gctgcaacgt
     6721 ttccttcctc gctacaaacc tttcttctgc gctaagctta ttaaaggtcc gttcgattcg
     6781 aaacatcttg tcacagaagt ctaatcctat tttggcatga ctttttgggg acggttgttt
     6841 acccaatgct tcaaaaaact ttctgcgaac atgcgcccaa caataaacat tgtttaccgg
     6901 cagctttcca taggccgcgt aaccatcagt atgcaagaaa ccgctaaatg attctaaaaa
     6961 actttttggc tcatcaacgg aacgcgaatt tgcgtgctcg tagagcacaa cttgtttttc
     7021 ttcgctttta cctgaagcat ataaccagta aaaggtcttt tctttatgag tattcaatac
     7081 ccgatagctt gtctcatccg cgtgtaagac ctcctgattg cttaattctt gatgaaggca
     7141 attgtagatt ggctctaaaa ccatttcact taccttgata tgccaattag tgattttttt
     7201 acgatcaaga gggaaaccaa gtgcatgcca ttctttttct tgtcgatatg ccggcacctt
     7261 tttaacaaat ttttcccaaa tactctgtgt cacaattgaa gaggaaccta gactattatt
     7321 gatcaacggt ttaggaacag gcgctttttt gatttttgtt tctcctaatg cttcacattc
     7381 tctgcaatga taggcatgtt ggtagatgac ttgtttttct atatgggcag gaacatacaa
     7441 tggctcttct ctaagccttg tcgcaccgat atcatgtaaa gtatgaccac agtcttcaca
     7501 caaacaatcg gcacctttta attcataatg aataggaact tcaggcagac cttctaatga
     7561 aagtgctttg agtcccttgc gttttctgcg tttaaaagaa gataccgtaa tgggttcctg
     7621 gctttcttgt ccagtgtgct ctggttcatt aaaagttgag tcgtctaata aatcgatttg
     7681 cccatctacg ggtggtgttt ctttttttcg tccaaacaag cgtcgattca tttcagcaat
     7741 ttgttctcta agaagagtat tttcctgttg ctggatttca agcaactgtg ttaattgctt
     7801 gatttgtaca agaagttcag attccatttt tgtcattttt acaccaccat tctagcgatt
     7861 ttcttacatc tattatacca ttttatgaca acgaaaaaca gacaaaatcc ttgatttaac
     7921 aaggttttcg tctaatatat cgcacctttt ttcgtttctt ttagcgtaca tttttcttcg
     7981 attgaaaggc cagtcagtaa gacttctttt ctggaacgcg gccattgaag gtacccactt
     8041 tcaaatcttt tgtacattaa tacaaatcca ttttgatcct agtaaagagc cttgaagcga
     8101 tctttcttcg taccgcaaaa taagaaaagc gcttgatcgt aaacatcaag gtcatattct
     8161 tctataacga aagctgctag tccatcaatt ccttttcgaa gatctgtttt tccacaaacg
     8221 ataaaaatat gtttcaatgc agttgcatca atgagcattg gcattcaact cttttaacaa
     8281 ggtatacaaa agatatttat ccattccatt ataaataaat atttccttat cttccgattt
     8341 gattctgcaa gctagttttg gcttggtgtc aatttgcgat ttttgagccg cacttgaaaa
     8401 ttgaactggt acaatatctt ttttattcat tcgtttttct ccttctatat ttgatagttt
     8461 aattatagaa gaaagataat cggcgtacga gatactatcc tatgacacgc ttacatttaa
     8521 tttaatattt tgcctcaaat tagccacact ttttatagaa ctaagaggaa aaatgaagtt
     8581 ttcttcctat taatatagtt gtttgaaatc ttaaatatag cgttttacga tgtaatggga
     8641 agtatggtga ataaatcaac tatgttcgta cgaagcattt actcaactaa tttaatcgaa
     8701 tctttcaata aacaaatcaa gaaatacagc caccgcaagg aacagttcca aaatgaagag
     8761 tcgatggaac gtttcttagt ctcgtctttt gatacttaca accaaaaatt cctaggtcgc
     8821 agtcataaag gtttccaaca ggccgaaggc gagctcgaac aaatgctgag tcaaccgatg
     8881 gagaattaga aacaatctac agtagggaag aaccattgac acaaaattat tgacgctccc
     8941 aactaaaagt gtcaaaataa tgatgaaatc atttagttct tataaattag aaaagagaaa
     9001 ataaatattc catatctact ttattggatc cgtaacatga ctaaattctg aatacttttt
     9061 aaagcttatc atttggtaaa actgaatata agttaattat atagactgaa aattttgaat
     9121 tttcagaata gaataactta aaagtactga caaaattttt taaaggggca atattaatgt
     9181 tctgagataa tcaaatccta ccgaaaatat caaaaatcca atgtgacaca atcggattgt
     9241 gtctcattcg agggaatcac acaatcccac gtggctctag gcagaattga atttttgctt
     9301 ggggcttttt ccttatattg attttgaatg agacttatta tatgttataa tgcaattaag
     9361 tatcattgga gagggcgaag aaaatgaaga cggtagaacc tattcgagac aaaaaaaaga
     9421 tcgatgcgat gaaggcaatc ttggcttcgg gaaaatatgg ccaacgaaat ttggttcttt
     9481 tttcgattgg gatcaacacc gcctatcgca tttctgattt gcgtcaattg aagctggccg
     9541 acgtgctgga aatttcacgc ggtcgcgtgg tggtcaaaga tcgattagcg atgaaggaac
     9601 aaaaaacagc taaacataat tcggtctata tttccaacaa attgcgaaaa acgattttgg
     9661 agtacgttca acaggaattt cctgatcaac tccttcagca agattttgac ggttatttgt
     9721 tccctagtcg aaaagggacg aatgaaccgc tcaaccgcca aagtctgtgg cgaatcattc
     9781 atgatgctgg cacggcaatc ggattgaaag acattggccc acattccatg cgaaaaacgt
     9841 tcggttactt tctctataaa caggggacca aaacagaaat cattcagtcc ttgttgaacc
     9901 attcttctca gcgcgaaaca ttgcggtata ttggtattac acaagaagac aaagatacag
     9961 ctgtaaaaag cttagattta taatataaaa gttaagttta aacatggata gttttaaatt
    10021 agtagattga gtgttgggag gaaagaatga gtacattata tattatagga aacggttttg
    10081 atttaagtca tggtttaaaa actagataca ctgatttttc aaaatgggtt gaaaaaagtc
    10141 atagtgaaat attcgataaa atagacacaa tgataaatga aaataatgag gtgagaagta
    10201 cagctgatga taagataggt tggtttaatt ttgaaagtag tttgcatgag ttaattcaaa
    10261 gtccagtctt tgaagattat gtagaagagt ctaaggatta tctcataggt tatggacatg
    10321 aagattggtc agattctgcc catcatgatt ttcaaaatat ggtttatgat tatgttgttt
    10381 ctctagaaga attacaacaa cttttcaagg aatggatacg caaagaaata cagccgacaa
    10441 tagaacataa aattccaaaa taccaatttt cgtccgatgt actattcttt acattcaatt
    10501 atacagatgt tttggaaatt ctatacaagc aaccgaaaga aaacatatat catattcatg
    10561 gcagttatga cgatataata atcggccact ccgagaattt tgacaacttt ataggagat
//
LOCUS       DAZUMO010000087        10603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000087 DAZUMO010000000
VERSION     DAZUMO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.087"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACG8ER_002428"
     CDS             23..646
                     /locus_tag="ACG8ER_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050960.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8ER_002429"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8ER_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5050961.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACG8ER_002430"
     CDS             2142..3023
                     /locus_tag="ACG8ER_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5050962.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACG8ER_002431"
     CDS             3026..3796
                     /locus_tag="ACG8ER_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5050963.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8ER_002432"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8ER_002432"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS5050964.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACG8ER_002433"
     CDS             5107..6270
                     /locus_tag="ACG8ER_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5050965.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACG8ER_002434"
     CDS             6387..6797
                     /locus_tag="ACG8ER_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5050966.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACG8ER_002435"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACG8ER_002435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5050967.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACG8ER_002436"
     CDS             9370..9654
                     /locus_tag="ACG8ER_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050968.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACG8ER_002437"
     CDS             9814..10050
                     /locus_tag="ACG8ER_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5050969.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACG8ER_002438"
     CDS             10047..10445
                     /locus_tag="ACG8ER_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5050970.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3703 a         1771 c         2241 g         2888 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaa
//
LOCUS       DAZUMO010000088        10551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000088 DAZUMO010000000
VERSION     DAZUMO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.088"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1220
                     /locus_tag="ACG8ER_002439"
     CDS             105..1220
                     /locus_tag="ACG8ER_002439"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5050971.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            1217..2632
                     /locus_tag="ACG8ER_002440"
     CDS             1217..2632
                     /locus_tag="ACG8ER_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5050972.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            2665..3612
                     /locus_tag="ACG8ER_002441"
     CDS             2665..3612
                     /locus_tag="ACG8ER_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5050973.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(3652..4812)
                     /locus_tag="ACG8ER_002442"
     CDS             complement(3652..4812)
                     /locus_tag="ACG8ER_002442"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5050974.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KELNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            4977..6152
                     /gene="mltG"
                     /locus_tag="ACG8ER_002443"
     CDS             4977..6152
                     /gene="mltG"
                     /locus_tag="ACG8ER_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS5050975.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            6288..6767
                     /gene="greA"
                     /locus_tag="ACG8ER_002444"
     CDS             6288..6767
                     /gene="greA"
                     /locus_tag="ACG8ER_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS5050976.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            6935..7666
                     /gene="liaF"
                     /locus_tag="ACG8ER_002445"
     CDS             6935..7666
                     /gene="liaF"
                     /locus_tag="ACG8ER_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS5050977.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAV
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            7663..8730
                     /gene="liaS"
                     /locus_tag="ACG8ER_002446"
     CDS             7663..8730
                     /gene="liaS"
                     /locus_tag="ACG8ER_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS5050978.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            8737..9369
                     /locus_tag="ACG8ER_002447"
     CDS             8737..9369
                     /locus_tag="ACG8ER_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5050979.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            9509..10174
                     /locus_tag="ACG8ER_002448"
     CDS             9509..10174
                     /locus_tag="ACG8ER_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS5050980.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            10202..10495
                     /locus_tag="ACG8ER_002449"
     CDS             10202..10495
                     /locus_tag="ACG8ER_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS5050981.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         3612 a         1532 c         2101 g         3306 t
ORIGIN      
        1 tttacattta tcaaaaaaat tctttttagg tatattttaa aaaaatagct ttctataaag
       61 gagtgtttta tttgaaaata gctatactat ctccagcttt ctcaatggga ggagctagta
      121 tcgtggcctc aaaaattgca aaaggattaa tagatagtgg ccatgaagtt ttttttgttt
      181 cttacttaga aagtgaaaaa ccacaagtag aatctaagta ttatgattta tacgaaaaaa
      241 aaattttttt atttaaaaat ttttctcgat ttcataagat attagagtat agattgtttg
      301 gaggctttgt tcctagtaga tatataaaaa aagaaaaaga acaactaaaa aaagttctaa
      361 agaaagaaaa acctgatgtc atcatattca atacttttat acctagtgta ctattctccg
      421 aattcctaaa gtatgaattt ccaacagtga aattaattac ttggatgcac tctgatcctg
      481 attacagctt aaatcatatt gctaaatttt ataaaaaaga atatagaaaa agttttgaac
      541 gcgtggattc cattgtttgt ctttcgcaag atacaaaaga aaaactttca ttatacaata
      601 accatattaa agttatttat aacccattga cattaagcgg ctctagttta ataagtaacc
      661 ttgataaaaa aattatttca tttactgcca gatacgatat aaatattaaa gggtttgatt
      721 atttatgtga agtagctaaa aacttacctg ttggatgggc tataaggatt gctggagatg
      781 gtacagaaga agaacgtaaa gagctgaaaa gattaattga gaaaaatggt gtgttagata
      841 aaattatttg cgcaggtgca ttatttggag atgaattaat caatcattat atagaaagtt
      901 ctattttcat atctacttct agaagtgagg gactaccatt agtgttgata gaagcaatga
      961 cttatggatt acctgtaata agttttgatc atggtggtgc taaggaaata ttacaagatg
     1021 gaaattatgg aattttaatt aagaattttg acgtacaaga tatgaataat gagatagaaa
     1081 aattgtctaa taatcgttta cttatggaaa aatatcaaaa attctcttta gaaagagcta
     1141 aggattttaa tgaaaaaaaa ataattggtg aatggcaaac attgttaaat gaattaggaa
     1201 atgaggatgt tagaaattga ggacttcgat taaaaatttt aatttatgga aagaatggta
     1261 tctatatata ttttttatag ggatgattat tcgtgcaata aatttttata gtttattacc
     1321 gtccactttt gatagtattt tttttaaact tataggaatt tcaggagttt tgctattagg
     1381 gatagatttc ataattaaat ttaataaaag aaaagtaact tataacattt tattgatagc
     1441 atatatggtg attttagtaa taacgagttt tgttcataaa gaatatggtc taggagaaaa
     1501 cttaaagact ataatgtggg caatcttaca gtattttttg atttatcagt ttgcttttga
     1561 gaatagcgat aataaacgat tttttaaaag gataagttgg ggatttattt atagttggtt
     1621 tgttttagta tcaatgtcaa tggttctttt ctttggtaaa ttcggctatg agaaatatta
     1681 taatgctaga cataggatca gaattggttt tttagaatca agactgtttg ggacgttttc
     1741 tgatattaac aatgctgcta tagcatcttt agttgttatt gtcctaatct tattttatct
     1801 ttataataaa aaagtgacag gattaaataa aatcattttt ttaattaatc ttccattaca
     1861 atttatttat atagtattaa gtgggtcacg atctacctat gtggtaagta taataatagt
     1921 tgccatatca tcttttattg ttgtgttaaa acgaaatgga acaaaaaaag atttaaaaaa
     1981 ctatatcctt gctatagtat cttctattgg ttctgttctg attttattcg ctttgtttaa
     2041 tgcaactaag atactttttc aagaattatt acctcattta aaagaaataa atttttttaa
     2101 ttttacagag cgtcataaag aattaatgaa taatagtcaa catattaata atactcaaac
     2161 acctgataat actcttgtta ggaaagacgt agctgaaaat acagatattt ctaatgctag
     2221 aattactata tggaaaagtg catgggaaat atttaaaaca agttggctta tcggagtctc
     2281 tccgaaaaat gtagtatctt atgcacagtc aattattcca gatggatata ttgcgaaagt
     2341 aggaattgcc attcataatg catacttgaa tgtattaaca tcgacaggta ttctaggaat
     2401 aattcctttc atggcttttt taataaagag ttttgtgctg aatgtttatc gaatcatcta
     2461 taataaatgc attggtaaag attatcattt tgtttactgt ttagttattt tatcttatgc
     2521 actatacgca gctcttaaca atgaattagt ctatgaaaac actgtaggaa cgtttgtttt
     2581 ttggctattt ttaggaagag ttaatagcaa aaataattct agctattttt agtaataaat
     2641 tttatcttga aaagaggaga aaaaatgttg aattacttga ttaccggtgg cgccggcttc
     2701 attggctcga cgctcgcgaa ttatctagga aaagaaaaca atgtagttgt gatcgatgat
     2761 ttatcgatgg ggaaaaaaga gaatctggat gaatcgaagc acatcacgtt tattgaaggg
     2821 gatgtcgctg acaaaaattt gatggaaaag gtcatgaaag aataccattt cgattatatt
     2881 ttccatctag ctgctgtagc tagtgtggca gattctgtag aacgtcctct agaaactcat
     2941 cgtgtaaatt ttgacagtgc gcttttattg cttgaattgg tacgaaaata tcaatctggt
     3001 ttaaaacgat tggtatttag ttcatcagct gcagtttatg gcgatgaacc aacgttgcca
     3061 aaaaaagaag aatcggtcat ccgtccttta acaccatatg ccatcgataa gtttgcagca
     3121 gaacaatatg ttttggatta ctgtcattta tatgatgttc cgactagtgc tgtccgattt
     3181 tttaacgttt atggtccaaa tcaaaatcct aactctccat atagcggagt tatttcgatt
     3241 ctagtggacc gatacaaaaa acaattggca ggagaaaaaa cagaatttac tttatttggt
     3301 gatggtagcc aatctagaga cttcgtttat atagaagatg ttatacaagc acttttactt
     3361 gttgctaagg aagaaaaagc tttaggacag caattcaatg tcggtacagg aaaaagcact
     3421 acgctacttg aattgattca ttcaattgat caaatcttag gaacagagct agcgcttaaa
     3481 tatgaagcgg agcgttcagg ggatattcgt gattctttag cggatatttc aaagatccgt
     3541 tctttaggtt atcaacccaa atttgatata ttgaacggaa tggaacgtta tttaaagaca
     3601 gaaatcgatt gaaacaaaaa accagcatcc tgctggtttt tttgtctcga cttagctttt
     3661 taaattaaca ccttccatca gttcgtaaat ctgatttctt aaatcagtgg ccggcaattt
     3721 gatgttttta tcagattgga tattactttc catgacgatt gcatcttttg tgtctttttt
     3781 catcgccgct actgcatgga atcgattgat atttccctga gcaaggatat aatcatttcc
     3841 actgtaaaca ccgccgctgt aaggatgttt atcaccatta ttttgccaca atgagaaaag
     3901 tagttctttt ggtataatat cgccagacag aacactggta aagaatttgt tcaagtcata
     3961 aacagacata cttatatttc ctgtgcctaa ttcatttcgg atgtctgttt caggctcgac
     4021 caatgctttt gaataatcgt cagttgcaga catttggtaa gatacagcat gctgcgggga
     4081 attttttaca ttttcataaa aatctgtatg ttctagatgg agtggccgaa tgatttcttt
     4141 ttttaaaaga gtttcataag gctgattcat tagtttgatc aatacccctg taagcaatgt
     4201 ataatttaat ggttcataat gatacgtatc ataaccagta aatttcaaat gatgaagggc
     4261 aaactgaatg atctcactat ctgacatagg tttttttgga gaagcggttc gatgaagacc
     4321 agagcgcatg taaagcatat ttttgattga gatttgctga cttccaggaa tctgaggata
     4381 aaattcagat agctttgtgt caaaagttat cttgccagat tgaatttgtt ttaggataag
     4441 taaagcagtc atcgcttttt ggatcgaacc gatttgatag taaagagaag gagaattcaa
     4501 ttcttttatg cgtagatttg cgtacccata tcctttttct aatatcactc gattattttt
     4561 gcgtacataa atcgaaccga tataatctga tttttgcagt aattgatcaa ttttgctggc
     4621 aagcatctgc tcattagatg ttaaattgga agcaagttgt gcgggttccg ttttataaat
     4681 ttgtggttga ggttgttttg ttaattgcca tcctgtaatg atgcctgttg aaaagaagct
     4741 gacagccagc aacaaaactg cgagaaaaga aagagagatc tgctgttttt tttgatgttt
     4801 tccttttgcc atattaaatc cttcctatcc atttttaact atttttcatt gtaatcctca
     4861 aaaagagcat ttgccactta caattttgac ataatatttg gtaaaataat tcacggcagt
     4921 agaaatttca tgtataataa acagagttac gaagatgata gtaaggagga gttgttttgt
     4981 caggaaacaa acaacaccca agcaaagaca aaaaagaagc cagtaaggca gctacagcag
     5041 atataatcga caagcggaga aatctgcgta aaaaagaaga taagatcgtg aggaagatca
     5101 ttttagtgat cgctcttaca ttattgatta ttggcggatt tctcgggttt accgtatatc
     5161 gttatgtaga tagcgggttg aagcctttag ataaatcgga cgaccaatta gtccaagtgg
     5221 aaatcccaag cggctcttct aataagcaga ttggcgaaat tttggagaaa gacaatatta
     5281 ttaaaagtgg gattgttttc aattattata caaaatttaa aaacttaact ggctttcaag
     5341 ctggttatta tcagttagcg cctaatatga ctcttgatga gattggcaag cagttgcaag
     5401 aaggcggcac atctgagcca acaaaagttg cagatgggaa aattgccatt ccagaaggct
     5461 acgacatcga tcagattgct gagcgggtag cgaaggtaac tggtaaagac aagaaagaat
     5521 ttttagattt agtaaatgat gagactttct ttaacgagtt gtatcaaaaa ttcccagaat
     5581 tgttggaaag tgcctctaaa gctgagaaca caaaataccg tttggaaggc tacttattcc
     5641 cagctactta tgattactac acgagtactt cgcttaagga tcttgtgatt gagatggtga
     5701 acaaaacaaa tacggtcatg caaaactatt attcagctat caaacagaaa aatttgactg
     5761 tacaagaagt tttaactttg gcttctctag ttgaaaaaga aggcgtaaaa gaaaatgatc
     5821 gtaaaaatat tgcacaagtc ttctttaacc gaatcaaggc aaatatgcca cttcagtcag
     5881 atatctctgt tttatatgca ttaggagaac ataaagaatt ggtgacgtat gaagatacag
     5941 ctgtagattc accatacaat ttgtatacca ataccggcta tgggcctggt ccttttgaca
     6001 atccaagcga agaggcaatc aaggcagtat tagaaccagc agaaaatgac tattattatt
     6061 tcgtagcaga tacgagcact ggaaatgttt atttcgctaa aacgtatgaa gaacatatgg
     6121 aactagtgca aaaatatgta aataattctt agaaaaacag gggctagtca ttgatcattt
     6181 ttagaaaaac atttacattt taagaaaagc gtggtaatct tttttggact atattcctca
     6241 aaagattacc ttttgcggta ttttatcgaa aaaaggagac gttacacatg gtagagaaag
     6301 tttatccaat gactctagaa ggtaaagaaa aattagaaaa agaattagaa gaactaaaaa
     6361 cagttaaacg cggagaaatc attgaacgaa tcaaaatcgc tcgcggtttc ggagatcttt
     6421 ccgaaaactc cgaatacgaa tcagctaaag atgaacaagc atttgttgaa ggaagaatca
     6481 caacaattga aaatatgatc cgttttgcac aaatcatcga taatgacggc gtagatgcag
     6541 atgaggtatc gatcggtaaa acagttacgt tcatcgagtt gcctgatgga gatgaagaag
     6601 agtacacgat tgtcggaagt gctgaagcag acccgttttc tggaaagatt tccaatgatt
     6661 ctcctatcgc gcaagcattg attggcaaac gtaaaaatga tgaagtaacc attgctacac
     6721 caggtggcga tatgttagtt aaaatcgtca aagtagaaca agcgtagtct gactgaaaga
     6781 tctagagagt tcatcttact gatgacgcac tctagatctt ttctctttga gtattgcagg
     6841 tttcactgaa cgatcttaca tctaaagtcc tatagccaac agtcaaaaaa ataggtatga
     6901 tgaaatgaga atgtgtcagg agggagaaaa agcgatgaat agttcttggc gtttttttgt
     6961 ggtagtcgaa gcactgctgc tgttatttgc tgtttggcaa atcgtaaaca atactggact
     7021 tttgctttta gtaatattcg gtattttcaa tatatatcta gctgtgcgca aatatcctcg
     7081 tactaaattt caaaattttc aattgatctt agggagcttg gtcatttttt ttagcttagt
     7141 caacagtcct gctttatgga tgatggttgt attggccgtt ttatttatcg ggctgaaagg
     7201 tgtagagatt tcaggaatag atttgacaaa aaatgctttt tggcgaaaaa aacagatcat
     7261 gatggtccaa acagagcaat taaaaacaca taataatgag cgtaaaagac agcaattatt
     7321 tggtaaccaa cgtattggaa acgacgtcta tgaatgggat gatatcaaca ttgccatcat
     7381 ttctggtgat actatcatcg atttgggcaa tacacttttg ccaaaagatg ataatattgt
     7441 gattgtaaga aaagggatag gcagaacacg gatcttagta cccctgggag tggctattcg
     7501 tttagagcat gcgacattag taggaaatgt cttgtttgaa gaagagcagt tttccttaaa
     7561 aaacgaacag atcaaaatct gcagcagtga ttatgatgaa aatcctcgca gattgaagat
     7621 cattaccaat actttggttg gagatgtcga ggtgatacga atatgatggg aagaatatcc
     7681 aaagcaatgc tagctgttta ttcggggatt gcggctttcc ttattattct attttcactt
     7741 ttcacttatt tttatgccag taaccaaagt cattggtggg gagaattgct gcgtgcgcgt
     7801 ttattatacg ttccgcttat cttccatttg atggcaattt cattaggtgt aggattgatc
     7861 gtatttctat tgttatcact tattcaaaag gcaaaatacg ggaaaatcga agaaaagctt
     7921 cgtgcacttt cttctggcaa ttatgaatcc aaacttttgc atcagccgat cccaagcgcg
     7981 tcggatgatc tgtacatcaa agatattgat atggaaatta ctaaaataaa agaaaaaatg
     8041 atcgaagtat ccagtgaatt acagattatg actagccgtc ctcaatatgt agatggacaa
     8101 accaaagaag agattttaaa attagaaagg catcgattag ctcgtgagct tcatgattca
     8161 gtttcacagc aattatttgc agcgatgatg atgatgtcgg cattgacgga gcaagcagaa
     8221 aaaagtgaga caccagaaat gttccgtaag cagttgaaaa tggtggcaga gatcatcaac
     8281 gcttcccagt ctgagatgcg tgcgctgctt cttcatctgc gtccagtcaa tttagaagaa
     8341 aaaagtttaa aacaaggcat tgagcagtta ttgaaggaat tgcagaataa aatccagatt
     8401 tcgctaaaat gggaggtaga agatgtaaaa ttaactagtt ccattgagga tcacttgttc
     8461 cgaatcgttc aagaattgtt atcaaacaca ttaagacatg ctaaggctaa tgaattagaa
     8521 gtctatttgc ataaaataga taacaatctt ttattacgta tcattgacga cgggacagga
     8581 tttaatatga atgaaaccaa aacgggaagt tatggattga acaatatcaa agaacgagta
     8641 gctggtattg gtggaacagt aaaaatcatt agttttaaag ggcaaggtac gagcgtggag
     8701 accaaggtcc ctttgatgaa ggaggcatag ataaagatga taaaagtttt attagtagat
     8761 gaccatgaaa tggtacgctt aggcgtctct tcttatttat ctatccaaga agacattgaa
     8821 gtgattggag aagctgaaaa tggacgtcaa ggctatgaaa aggcgatggc acttcgaccg
     8881 gatgtcattt taatggatct agtcatggaa gaaatggatg gcatcgaatc aacaaaagca
     8941 atcttaaaag attggccaaa agccaaaatc attatcgtca caagttttat tgatgatgaa
     9001 aaagtttatc ctgcaattga agcaggagca gcaggatatt tgttgaagac gtctacagca
     9061 catgaaatag ctgatgctat aagggctacg caacgcggcg agcgagtact agaacctgaa
     9121 gtgacaacaa aaatgatgga aaaaatgagt cggcgaaatg atccagtatt gcatgaagaa
     9181 ttaacgaatc gagaaaatga aattttgatg ctgatttctg aagggaaaag taatcaggaa
     9241 atcgcggatg aattgtttat cacactgaaa acagtcaaaa cgcatgtgtc gaatatatta
     9301 gctaagttag aggtagaaga ccgaactcaa gcagctatct atgcgtttaa acatggtttg
     9361 gtgaaataaa aacgatgata aagaggataa tcaaaagctg agaaaataac tattattttt
     9421 cttggctttt ttacaataat gatgcttgct tttgtttttg gaaaggcttt gttatactga
     9481 gaaaagaaga tactaaagga ggaaaatggt gaaacaaaat tttgcaattg taggccttgg
     9541 acgatttggc ggaagcatct gccgtacact gatcgaagct ggacaagaag tactggctat
     9601 tgacagtaat gaagatcgag tcaatgaata tatgaatata gccacccatg ctgttgtggc
     9661 aaatgcacag gatgagatga cacttcgttc attaggtata agaaatttcg atcatgtaat
     9721 tgtcgctatt ggagaagata tccaagccag catcttagtt actctgatgg taaaggaaat
     9781 gggcgtaccg aatattttag caaaagccca aaatgagtac catgcccgtg tactagagaa
     9841 gatcggagca gatcgtgtcg tacaccctga gcgagacatg gggatacgga ttgcacataa
     9901 cttagtatca aagaatattc tagactactt agagctgtca gataaatatt ctttagcaga
     9961 aatccgagtc tccaatccta aatttttcaa caaaacatta gctgaattaa atttcaggca
    10021 acgttttgac ttaacagtag tcgccatacg aagagtagat caaacggtta tcgcttctcc
    10081 tgcagcagat gaatatgttc gtgaaaatga taatctactg gttattggag agacaggcga
    10141 tgtggatatt ctagacgata agatgaatca atagaaagaa aagagaaagt aggttgttgg
    10201 gatgaaatta acggtaacgc caaaagcaca aaagtggttt gactcagaaa ttgatctgcc
    10261 agaaacttac ggaatacgtt tttttggaaa agtctacggc aaaacagagg ttcatgatgg
    10321 tttttcgatt ggaatgtcag ttgaacagcc agaacatcct gtaaagaaag tggaaatgga
    10381 tgggacactt ttttttatcg aggaagcaga tgaatggttc tttaaaggat atgatttact
    10441 tgttgattat gatcccaacc tggaggagcc aacctatcac tttaaaaaac aataaaaaaa
    10501 gagagggtga cacaattttt gtcaccttct cttttttctt ttgaaaatat t
//
LOCUS       DAZUMO010000089        10268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000089 DAZUMO010000000
VERSION     DAZUMO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.089"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(559..1599)
                     /locus_tag="ACG8ER_002450"
     CDS             complement(559..1599)
                     /locus_tag="ACG8ER_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5050982.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="ACG8ER_002451"
     CDS             complement(1948..2274)
                     /locus_tag="ACG8ER_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050983.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="ACG8ER_002452"
     CDS             complement(2261..3049)
                     /locus_tag="ACG8ER_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5050984.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="ACG8ER_002453"
     CDS             complement(3417..3707)
                     /locus_tag="ACG8ER_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS5050985.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3843..4022)
                     /locus_tag="ACG8ER_002454"
     CDS             complement(3843..4022)
                     /locus_tag="ACG8ER_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050986.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4019..4369)
                     /locus_tag="ACG8ER_002455"
     CDS             complement(4019..4369)
                     /locus_tag="ACG8ER_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050987.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4362..5687)
                     /locus_tag="ACG8ER_002456"
     CDS             complement(4362..5687)
                     /locus_tag="ACG8ER_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5050988.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6137..6751
                     /locus_tag="ACG8ER_002457"
     CDS             6137..6751
                     /locus_tag="ACG8ER_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5050989.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6916..7920)
                     /locus_tag="ACG8ER_002458"
     CDS             complement(6916..7920)
                     /locus_tag="ACG8ER_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050990.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(8379..8636)
                     /locus_tag="ACG8ER_002459"
     CDS             complement(8379..8636)
                     /locus_tag="ACG8ER_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5050991.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8629..8898)
                     /locus_tag="ACG8ER_002460"
     CDS             complement(8629..8898)
                     /locus_tag="ACG8ER_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5050992.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9029..9208)
                     /locus_tag="ACG8ER_002461"
     CDS             complement(9029..9208)
                     /locus_tag="ACG8ER_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050993.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            9306..9542
                     /locus_tag="ACG8ER_002462"
     CDS             9306..9542
                     /locus_tag="ACG8ER_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050994.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(9589..>10268)
                     /locus_tag="ACG8ER_002463"
     CDS             complement(9589..>10268)
                     /locus_tag="ACG8ER_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050995.1"
                     /translation="EDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFIQRVEEQLYLE
                     SETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKYIMNVYLERSELVENFVSYT
                     ICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLTCGTESIVLMG
                     MDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQR
                     FERKKRQ"
BASE COUNT         3132 a         1876 c         1472 g         3788 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataaccccaa
     3961 atctcttctg atagctgatc aaaggttata tactttagct catattgctg gataatgttc
     4021 attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt
     4081 gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc ttgccaatga
     4141 tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat actgatctat
     4201 tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt tgttcttttg
     4261 ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta tgatcagata
     4321 aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt tttaattccc
     4381 ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct agcgccttct
     4441 aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat cttgtcaata
     4501 attttatcta aattttcttc gtcaatttgc ttttgtggat cactaaataa atccagctga
     4561 agcgaatcgg tgtacacaag cttgctatag gttattccaa catgccttac ttcttgtcct
     4621 tcataatact tatcaaacaa gaaaagacaa taattctgca attcctttgt attatctgtg
     4681 atagggatct tcatctggtg tgaaaaccct ggtctcgttt ctaagatcga cgtgccaatg
     4741 tgtaaatgga cacaacctgt tttacagtta tgctgcctaa tgcgtatcgc tacttgttcc
     4801 gccatttctt ttaccaccag ctcgatttca tttcttctag tataatctcg tggtaacact
     4861 tgtgaatttc catatgattt ttcctttgtc ggttctggtg ggattgctat atctgttcga
     4921 tcgatcccat tagcatggaa gtacagctgt aagccaatca cacctagtct attcttaatc
     4981 gtataaggat tccagttagc aagatctcta atgctcataa tccccatctg attgagtctt
     5041 tttttcattc gagagccaat tccccaaaag tccgtcattt caggaatatt ccatactttt
     5101 tcaggaacgt tttcgtacgt ccattccgca acaaatcctt cattatgctt tgcttcgttg
     5161 tctaaggcta gtttagccaa aaggggatta tctccgacac ctactgtagc aatcaatcct
     5221 agctcttctt taatattctc ctggatcatt tgagccagct tctttctccg ttgacttcgt
     5281 gttccttcag tcgtaaaaag attcagtgat cgggtcactt ttaagattga ttcatcgatc
     5341 gagtaaatca gtagatcttc atcagccaca tatcttctga aaatattatt tacctgcata
     5401 tttcgcttga tatataggtt catacgtggt ggaacaacgt gtagtgtttt aggaaatggt
     5461 tgtggtaagt cacgtggtct actcacattt gtaataccat accgcttttt tgcttcagga
     5521 gaagaagcta atatcaatcc tgaaccagta ttatcagctc gactcataac aacaagttct
     5581 gttgttaatg gatctaaatt tctttctata cattcgacac ttgcgtaaaa gcttttcata
     5641 tcgattaaaa agtaatcatt tactggctct tttgaataat ccatcataaa taacactcca
     5701 ttctttcaca ttatacaaac atatgttcga tattgtaaag aagaaatagc taaatacata
     5761 aaaaaagaag cataggtatg ctatcaccct gcttcttctt ttgatttgta tgatcaattt
     5821 attctatcac tttactaatt aatttgaaaa tctaataaaa actctcactc taaaagagtg
     5881 agagactgca aattagagta cggacttttt tggagttccg ttctttaata ttaacaaatg
     5941 aataaaatcc ttacaacatt tgagaataag ttatcagtag acacttacaa aaaattctca
     6001 tactatttcc tccatcatta gtttttgtct atataaggtt taccctaaat agacactggt
     6061 ataacattga aaaatatggc ttcctcgtgt atgattaggg tgtattttaa atagttgcag
     6121 aaagtaggta aaagttttga gagttgcata catacgtgtc tcatcgatcg atcaaaatga
     6181 acaacgccag attgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca
     6241 atctggcgca acgattactc atcgacctgt tttccaagaa gcattagatt acgtccgaga
     6301 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattatg acgagatcat
     6361 cgcttcagtc aattacttga agaagaaaaa tgtgaagctg atcattacta gtttaccgat
     6421 catggctgaa gcaattggca atcctctcct ggataaattc attaaggatc tgatcatcca
     6481 aattttggcc atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc aggcacaagg
     6541 aattaagatc gcaaaagcaa acggcgtcta caaagggcgg cccaaattat acagtgcaga
     6601 cgcaaaagat ccccaacgtc gccttgttta caaaagcatc gttgaagatc ttaaaaatgg
     6661 tgttgcgatt gctaagattg ctaaagacta caatgttacg agacaaaccg tgtatagaat
     6721 aaaaaaagac agcatggtca atgacaaata gttgatcatg ctgcttttat tatcttctca
     6781 tctttaattt cctacatata attactatct ataaaattaa gtcttactca gaacagataa
     6841 agcataaaat tccaacaaac cattttttac ttaactaaat aaagagtctg ttaatataat
     6901 cttcacttat ttacgttaat cccctactgt atttgttggt tccttatcta tattttctac
     6961 aaaatcctta ttatcttgtg atttagcttc gtataattct ttgtatgtcg acacattggt
     7021 tatactaatt cctatagcta gttcttgaga ctctactttt ctctctagct cctttgacaa
     7081 agtgatttga gatttatttg gttccgttgg agtcaattct atagattctt cataaaaatc
     7141 tctatacata aagctatcat taattttata aaaaaaatta aatgttttta gcactgccat
     7201 ataacattca aaacattctt ctatagttgg tttacttcct tgaggagaat gaaccaattt
     7261 attacgtttt tctatgaaat ttttaatttc ctttcttatt tctttcggaa actcaatatt
     7321 aaagtaccct ttaaacatgc tccctaataa tttgtctaca gggggagatt gtaaattctc
     7381 aaatattaga gttaaatcag gtttctcttg ttgataaaat tcctttaccc ctatttcaca
     7441 ggctgtaatt atcgaaataa acctatctct agtatttgat atatttttac ttcttttaag
     7501 gagtataaga gaaaagggga tactttcgtg tccatgcatg taattaacta attgttgagc
     7561 ttcagaagtt atatctctaa ttatatagtt aacggaatta ggaagtgtct ttaactctaa
     7621 tgaaatttct atccaattac tatcttctaa tctgtatatg ctcgaagtat aatttgattc
     7681 gtcagcaagc atcgctacat cccaaacact gatcataaaa tcttttaaac gttcaatcat
     7741 ttcagcaata ttgctaaaat cagctaattt ttctttagaa taaaaaaaaa catttatatc
     7801 agaatcaata aattctattt tttttattac tatatcgtca tcattatctt gaccatttat
     7861 gtttattgtt ccctgagtgg ggagtacttc tttcaaattc aagttaaatt tatattccat
     7921 tacttctcct ccaatatata aaaattactt aactgtatcc aaatttaata tgtaaataaa
     7981 tcattaattt ttagctaaat tctatgagaa gtttattttt gttgtttttg ctaattaact
     8041 aaaactagta caaaaaattt aaaatagatt attaaaccta ttgcttattc tattcagtca
     8101 tatatataag cgtcatcctt tagtaactaa catcccacac tacaatagta ttataacata
     8161 tctattcaac ttcaaattgt tgaaatttaa gccatttata tttacttttc ctcaacttta
     8221 gaaatgattt tactaacctt atatccgtaa tacgcgtaac aaacacattc attaataaca
     8281 atcaacacta agctatatta gctttttaaa tgactagggt aaataaaata aggatcaaaa
     8341 acttttaagt ttctgaccct ttccttactt ccgattggtt aatagtgatc ttttgcagaa
     8401 taaataaata tcgtttcatt ttcaactcta tatatcagtc tatgttcatc tgtaattctt
     8461 ctggaccatt ttccagataa atcatgcttt aatggctcag gttttcctaa tccagcaaag
     8521 ggggaacgat cgatatcttt tattaacttg ttaatctttt ttatattgct tttgtttcct
     8581 tgctcatgcc aataaagata atcatcccaa gcatcatcag accaagcctt aatcatcaga
     8641 ttcaacctcg attaagtcat gtgttttaaa tgcacctttg gagaattgtt catctcctcg
     8701 acgaattttt tccatgacgt aattattaga aagtgttctc aacgtttctt gcatagaatc
     8761 ataatctctt tttgataata caacaactgt atcttctaca tctttacttg ttacaataag
     8821 tgtttcagca tcctcattaa cttgtttcat ataactacgt aaattttggc ggaaatttga
     8881 ataagctact gcttccattc ctttcacccc ttatttattt ctataaaaca attgtacatt
     8941 atattgtaca attaagcaaa tgattaatat tctaacttct tataattaaa agtgctatca
     9001 cttttagttt ttaggataaa aagtgacgtc actttttgag tgctggtacc tcaaatcctg
     9061 ctttttcttg ggcaatcaat aaatctaacg caatacacat aatcctggat tcacttttgc
     9121 cgtacttctg ctttagttgc tgcaattctt tttgtctctt ttcatccaac gtaacgggta
     9181 accgtttatt tcctattgca attggcatta gtactctctc ctttcatcga aataacccta
     9241 tgaaaaatat ggctgcaatc atcttgaata gtagactcac aagggctaat acaaagcgcc
     9301 aaaagatcaa acaaaatacc agtattgcca aaaatactac gaagtttaaa tgcacagctg
     9361 cagcatggcg cataagtatt tcgccaacaa ataggactaa caatagagga atgattttat
     9421 tcatatggat ctccttcttt ctcttaggtt atcgggtgtt gcccgcttat ttcgcaccac
     9481 ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa gaaccattcc cttagttact
     9541 aggttttttg tccttatttc acacattctc acttttcggt ataaatgatt attgcctttt
     9601 tttcctttca aatcgttgat atggcattcg tttacggtca agttcatttt ggatttcttc
     9661 tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa gtcgtgtacc agtcgtcgcc
     9721 tgggttatct acttcttcta catattccct caaaaatgct tcgggtacag cgagttcata
     9781 atccattccc attaacacga tagattccgt tccacacgtc aaaaatctag taaatacaag
     9841 gtaataatcg tctatcgtcg catctttgct gtttagctct tccatttctt cttccattaa
     9901 ttccatgctc tcaaaaattg aataatattc tgattctgag taatagtgtt tcctgcaaat
     9961 cgtgtagctg acaaagtttt cgaccagctc actcctttct aggtaaacat tcatgatgta
    10021 tttttctgtt tctagtcgta gaagctccgt tccttttttt accgtttcag gacgacgttt
    10081 atacacgtct aaacgattat ggctgactgt gaccttcgtt tgactcgttt cactttccaa
    10141 atacaattgt tcttcgactc tttgaataaa gtacttcatt tcttccatac aactaaagtc
    10201 tttttgaatc cgtctgatta cttcattcaa atgttctttg tttgttaaaa agtgtgacac
    10261 atcttccg
//
LOCUS       DAZUMO010000090         9973 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000090 DAZUMO010000000
VERSION     DAZUMO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.090"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..408
                     /gene="rpsN"
                     /locus_tag="ACG8ER_002464"
     CDS             139..408
                     /gene="rpsN"
                     /locus_tag="ACG8ER_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5050996.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            423..602
                     /gene="rpmF"
                     /locus_tag="ACG8ER_002465"
     CDS             423..602
                     /gene="rpmF"
                     /locus_tag="ACG8ER_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5050997.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            634..1011
                     /locus_tag="ACG8ER_002466"
     CDS             634..1011
                     /locus_tag="ACG8ER_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5050998.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1032..1181
                     /gene="rpmG"
                     /locus_tag="ACG8ER_002467"
     CDS             1032..1181
                     /gene="rpmG"
                     /locus_tag="ACG8ER_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5050999.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1205..2188
                     /locus_tag="ACG8ER_002468"
     CDS             1205..2188
                     /locus_tag="ACG8ER_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5051000.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2218..2340
                     /locus_tag="ACG8ER_002469"
     CDS             2218..2340
                     /locus_tag="ACG8ER_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5051001.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2358..3881
                     /locus_tag="ACG8ER_002470"
     CDS             2358..3881
                     /locus_tag="ACG8ER_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5051002.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3905..4144
                     /locus_tag="ACG8ER_002471"
     CDS             3905..4144
                     /locus_tag="ACG8ER_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051003.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4337..4798)
                     /locus_tag="ACG8ER_002472"
     CDS             complement(4337..4798)
                     /locus_tag="ACG8ER_002472"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5051004.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4993..5211)
                     /locus_tag="ACG8ER_002473"
     CDS             complement(4993..5211)
                     /locus_tag="ACG8ER_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051005.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5329..6945
                     /locus_tag="ACG8ER_002474"
     CDS             5329..6945
                     /locus_tag="ACG8ER_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5051006.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7186..8526)
                     /locus_tag="ACG8ER_002475"
                     /pseudo
     CDS             complement(7186..8526)
                     /locus_tag="ACG8ER_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8964..9110)
                     /locus_tag="ACG8ER_002476"
     CDS             complement(8964..9110)
                     /locus_tag="ACG8ER_002476"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051007.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9149..9343)
                     /locus_tag="ACG8ER_002477"
     CDS             complement(9149..9343)
                     /locus_tag="ACG8ER_002477"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5051008.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9253..9585)
                     /locus_tag="ACG8ER_002478"
     CDS             complement(9253..9585)
                     /locus_tag="ACG8ER_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5051009.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3316 a         1587 c         1980 g         3090 t
ORIGIN      
        1 agttatctat tcatatgagt tttatttcat gttatttatt gttgacaaga gcaactcttg
       61 ttggtaaagt atcagtgtca aaacgtaacg attacgattt gaggtgtcta aatcaaaata
      121 aaaggagtat aaaggtatat ggcaaaaaag tcgaaaattg ctaaagcaaa gaaacaaatg
      181 gcgatgattg aaaagtatgc agataaacgt caggaattaa aagccgcagg agatcgcacc
      241 gctctcgcta aattgccaag agattcaaat ccgaatcgtc tgaggcttcg tgatcagacg
      301 gatggtcgtc caagagggta tatgcgaaag ttcggtatgt cccgtattaa gtttagagaa
      361 cttgcccatc aagggttgat tcctggagta aaaaaagcaa gttggtaaaa ggaggaaata
      421 tcatggcagt accagcaaga aaaacatcta aggcgaagaa acggttaaga agaactcatc
      481 aaactgtagt aaaaccagaa atttcatttg acgaagcgaa tggtgattat agacgtagcc
      541 atcatgtatc tttaaaaggg tactataatg gaaagaaagt aattaaagga gcttcaaaat
      601 agtttaatat ataactttta ggagggaatc aaaatggaaa ttatttaccc acctttagtt
      661 gaagaaggat taaagtatta tcttgaaacg acccagcaat cgctagataa atgcactttt
      721 tatcgttcga tggtagagag aggaattatt actgaaacgg gtttaccgac ccaacaggca
      781 attgaaaatg gattagtcaa agattactat gaggaccaag gcttatcatt tgatgatttt
      841 ttaagaatct atccaatttt tgaagagtat gacgaggagt tatttcaatg tatcgatggt
      901 tattgggaga tacctgttga tatgaaagaa aatttagttt cacaattgga atctggcgag
      961 ttaacttttg aagatgcaca acaaattcaa gcatatcttg aagatcgata agtattgaag
     1021 gaggaagatt tatgcgtcaa aatattattt tggaatgtgt cgaaactgga gaacgtctgt
     1081 atctcaccag caaaaataaa cggaataacc ctgaacggtt agaattaaaa aagtattcgc
     1141 caaaattgcg cagacgggca atttttaaag aagtgaaata gctaagtggt aaaaaggtga
     1201 caaaatgggc ataccagtta caatcgtttc tgggttttta ggggctggaa aaacaacgct
     1261 aatcaatcaa gccttgcaag tctcacccta ccccagggaa gaaattatta taattgaaaa
     1321 tgagtttggt gaagttggga ttgatcacaa attattagtt catagcgagg aacgggtgtt
     1381 acaactaaat aatggatgta tgtgttgcag tcttcgcagt gatttattag caatattgat
     1441 ttctttactg gaaacaatag aagaacatca acaaccaatt tcacaaatca tcattgaaac
     1501 tacaggtata gcagatcccc aaccaatcat tcaaacactt ttaacagcgc ctcaactaag
     1561 aggaagagta tatatcgata gtttattgac tgttattgat agtgacagtt ttgaacttat
     1621 tgaaagagaa ccacaagccc ttaagcaact tgtcatggca gatcgcattt ttgtgtctag
     1681 aaaacaaaac ttgaatttat ctgcactaaa aaaattacag agagaaatca aaagtattaa
     1741 ccctttatcc gatattcgaa tgttcacaca tagtgaaacg attcaaaaaa aggattttta
     1801 taatttgaaa aaatttaatc atcccttagt ttttgaaagt gattcatcag aaaatcacta
     1861 taatggcaaa tactatcatc aacaccatca agtacatgag tttaaatcaa ttctcttaac
     1921 ttcagatcaa gacataaaaa aagatttttt actccaatgg attgactggc tgattttaac
     1981 caaccaaggg agcctgtatc gttttaaagg attgattgct ttacaagagc aggattttat
     2041 agttgccatg caagaggtaa acgaacaggt taattttcaa tacaccactc gacaaaaaga
     2101 ccaaaccact attattctaa tcggaaaaat gttggatgaa gaaaggatca aacagagttt
     2161 tgataaatta ctggcggaaa gcaaataatg ataagtagtg attgggggta atttctaatg
     2221 gagaaaacgg atatatctag tgcatatcga cgacttaaaa gcccgaatat taaaactaga
     2281 aaaagggcat taaaaattat caaagaacat aaacgaaata aaatgaagaa attagcataa
     2341 ttaaggaagg aaaaagaatg aagaaaatca gtgtaggact tattgggata gcagctctag
     2401 gattattggg agcttgctcc agcactaatg atgcaaaagt atcgaatgat aaagatggaa
     2461 aattagaaat tgtaacaaca ttctatccaa tgtatgattt caccaaaaac atcgtcggcg
     2521 atgaagcgaa cgtggattta atggttccag cgggatcaga acctcatgac tatgagccct
     2581 cagctaagga tatggcaaaa gctcatgatg ctgatgtttt tgtatatcat aacgaaaaca
     2641 tggaatcatg ggtaccaaaa gctaaagaaa gttggaagaa agctgggcct aatgtagtag
     2701 aaggaacaaa agacatgatt ttattgccag gtagtgaaga agaagatcat gatcacggag
     2761 aagaagatca ccatcatgaa ctagatcctc atacgtgggt ttcacccaaa atggcaatta
     2821 aggaagtttc aaatatcaag gatcagttag ttaaattgta tccgaaaaaa gcaaaagtat
     2881 ttgagacaaa tgctgaaaag tatttgacta aactgaaacg gttggatgct gactatacca
     2941 ctagtcttaa agaagcgaaa caaaaaagtt ttgtgacgca acacgcagcc tttggatatt
     3001 tagctttgga ttatggattg aaacaagttc ctattgctgg actaagtcct gaagaagagc
     3061 catcttctgg tcgattagct gaattaaaag agtatgttaa aaaaaataag atcaattata
     3121 tttactttga aaagaatgct aatgataaaa ttgcaaaaac tttggccaac gaagctggga
     3181 taaaattgga agtactgaat cctcttgaaa gtcttacaaa agaacaaatg gataatggtg
     3241 aagattatgt ttcagtaatg gaagacaatc taaaagcgtt agaaaaaaca actatggttg
     3301 ctggtgaaga ggtagttcca gaaaaagaag cgaaggatga aaagacagtt gctaatgggt
     3361 actttaaaga tgccgatgtg aaggatcgcg aattgagtga ttatacaggc gaatggcaat
     3421 ctgtttatcc gttgttaaaa gatggaattt tggatgaagt tttcgattac aaagcaaaaa
     3481 tcaataaaga catgacagct gccgagtaca aagattatta tactactggc tataagacag
     3541 atattgatac tatcaacatt aaagacaaca ccattgattt tgtagtcaac ggtgaacatc
     3601 atcaatacac atacaaatat gttggataca aaattttgaa ttatgaaaaa ggaaaccgtg
     3661 gtgttcgttt taattttgaa actgatgatg caggtgctgg tcgttttaaa tatattcaat
     3721 ttagtgatca tggaattgca ccaagtaagg cagagcattt ccatattttc tttggtggag
     3781 aaagtcaaga aaaactctat aatgaattgc ataattggcc tactttctat ccagcttcat
     3841 tgtctaaaca tgaaattgct caagaaatga tggctcatta aaattcaatt tttggaggaa
     3901 atacatggta agaaaagtat tattgtctat ggattccaag aatattgatg cttatcggcg
     3961 ttatcgattt gcaaagcaaa tgagattaaa tgggagtaca ttagctgata ttgctgcaac
     4021 tttacatgtt agtattgaca cagttcatag agtattaaaa cgggatcctg attgttgttt
     4081 tatcaaggtc aatactggaa gaagtagacg tttagcaaaa cggcataata gcttggccaa
     4141 ttaattgata ataccagagt agatcatttt tcccttattt ggatttgatc tgctttttat
     4201 tatgtcttga attgtcaaat caagcgcaac actttatcaa agaagacttc ataaggtgtt
     4261 ctccaatcta aacactttct aggtctgtta ttcagttttt caatcatctg atttatcgtt
     4321 gagttatcta ccgtagctat ctctttcgat tttggcaagt attctcgaat gagtctattc
     4381 gtattttaat ttgttcattg ttgccaaggg catgaggatc tggaaaataa aagggcatat
     4441 tattcaatgc ttctgtaact gcgaaatgat tggagaattc ttttcctcta tctggagtga
     4501 tcgttttgac cgcctcattt ggaatactgc ttaaaagttc aatcattttc tcgctcacat
     4561 aagcggcttt ctttctgggg accttctttc ctgctaaaag gaagcatgat tttctgtctg
     4621 tcaaagtcac taaacaagta ccaccagttt tgccagctac aatatcggct ttccaatgac
     4681 caaaatcact tctatcatct gcggattttg gacggccatg gatcgaatga gtaaggcaaa
     4741 tctttccacg agactcgaca tgattttttg tatgtcttgt ttttcctcga tggtgcaatg
     4801 aacggatcag gcctttattg ccgtgtgaga ggggttctgt atcgaaaaga tcattgcaaa
     4861 ttgcacgtta gatcgtgata aaactaattg aaaagtccat atgttcatgc ttcaaacgat
     4921 tcgagatttc ctctggcgac cactgaaaat cgacaaataa atgacgaaca attgaccaaa
     4981 ctcacggatc atttagaatg tgataacgtc cgcatttttt tcttcgataa gcataatttt
     5041 tttccgcttt ggaaggtgaa tagttttttt cttgtttctt ctcagttcat gtaaaatagt
     5101 aaacgggcta tgctgaatgt cgttgccaat tatactaata gaaaatcctt cttcgtgaag
     5161 cagaaagatt ctttctcgtt cgagtatagt aagatgacga tactggctca taattatttt
     5221 ccttctagga gtgttctcga caaacttata ctagttggat atcgattatg actcattttt
     5281 atgtgttgca cttaaagtgt aaattcaagg tatagaaaga ggttccctat gaaaaaaata
     5341 cctattacaa tcttaactgg ctatcttggt tcaggaaaaa cgatacttct aaatcatatt
     5401 ctgtacggtg atcacggaaa ggaaattgct gtcattgtaa atgacatagg ggaagtaaat
     5461 gttgatgggg tattagtcga caagaaaggt tttaaaagaa ctggagaaaa acttgtttcg
     5521 atgccgaatg gatgcatttg ttgtacatta cgcgaagatt tgattgttga actagatcaa
     5581 ttagtgcaat tttctgactt gaatcaaatt gttattgaag cttcaggaat cagcaaacca
     5641 attccaatta ttcaagcaat tatattagct aaaaaccctt tagacattga tttgacccaa
     5701 cgattagaga ttggtgccat tattatagta gtggatgtaa aacgtttttg ggataatttc
     5761 gaaacgggtg aaaagattca ggaaagaaaa gctgaaggta atgtagttga agaagaacag
     5821 gatattcgtg gcttgttaat tgatcaattc gagtgttgta acatattact tttaaataat
     5881 agtgacttag tgtcaaccaa gacgatttac aaaattaacg gtctaattgc taagttacag
     5941 tccaatgcca aaataattag gacaattaaa ggtgaagtag acttatcgaa aattctaaat
     6001 acaaatcttt ttaatataga tgagacttcc tattcagtgg gctggttcaa agagttagag
     6061 cttggctatc aaaatcatat tcctgaaaca gaagaatatg gtatctcaag ttttatctat
     6121 cgaaatcggt tactccttga cgcacaaaaa tttgcccatc ttacggataa aaattttcct
     6181 ggcaatatta caagagcaaa agggtttctt tggtttgccc aacatagtga tttttcgacg
     6241 atctttgaaa catcgggtag ctataaaagt attgaatcat taaattattg ggtagctagt
     6301 ttacctaaaa ttgagtgtca aatgattata caaaaaaatc ccgaattgaa ggaagcctgg
     6361 catccgttgt ttggtgaacg cattaataaa ctagttttta ttggtatgaa gatggatcaa
     6421 caatgtatta aggaacaact tgagggttgt ttaactgatt ctcttaatgc agataatttc
     6481 cgaaaaattt caccatttcc atggaaaaag tctaatatgt ttagccagat atcgttaatg
     6541 aaatttttaa gaaaagctgg tgtcaagata ggcgatgtca taattattgg cattttgatt
     6601 attacctctt ttattccctt atttgtattc tctatagaaa aggagaatga aacaactgaa
     6661 gatgctatca aatatgccat cgttcgaatt gatggcaagg aaatggatcg cttcgatttg
     6721 gataagatca atcataaact cgtgaattat catcctgcga agggacaata taatattgtt
     6781 gagattaaag atggaagaat tcgagttaaa gaagataata gtcctgatca aatcgcggtt
     6841 agaactggat ggatctcaaa acccggtcaa acgagtattt gtttacccca taagttagtt
     6901 attagtattg agaaaaaaga aagcacagat tactacattt actaatctgg aaatagatga
     6961 ggaggttttt aataagaaaa agatattatt attaataata gcaggggctt tattaagtgt
     7021 gttaagcgct tgtggacaag aaaaagaagc ggtgacaaca ggcacaagta tctttaacgg
     7081 gacctgcaat ggtttagtta acagtcggtt agattactta ttttggctct tcgtcaatga
     7141 gtgttggtga agaaattcaa tagaataatc gagatactca cacggctaga cagctatctt
     7201 gtctagccgt gttgttgttc tggctttagt cagttgggta gtattcttag ttttcttaaa
     7261 atattttcct cgatataaaa agattcgttc tcggtagttt tgaaagtttc tatagccata
     7321 ggcaattcgt tttagtactt tgatatggtt attcaaacat tctatagggc cgttggaata
     7381 gggaacgcgc aacgctcgtt tgatttctgt ttggtatttc ttgaaggtcg tgaacactgg
     7441 ttggtagtgc tctggcaact ccttgtagtc ttcttttagg agcgcgacga attcaggaac
     7501 atcttgtctt ctgataacgg aaagaaaatc ctggtagact tcgtagcctc gctttaagga
     7561 atcatcataa gataatagcc gatcgactat ctctgcttct gtcagatgat ctcgaaatga
     7621 cggacgccag tagcgatgct gacctttcaa ttcccacgca ttcttttgaa gtagtttcca
     7681 gtatttcttt aagtgcttcg accgacgatc ccctttttta aaggtcttca ttacttgaat
     7741 tcggtggttg gtaaaagccc gaccaatgtg ttggctaata tgaaagcgat cggcgacgat
     7801 tcgggcattc ggaaaacact ctttcaccaa tgtgcgatag ggtgtataga aatctgaaac
     7861 cactagacgc acttgttcac ggacttttat agggtagcgc aagaaatagt cgcgtaaagg
     7921 attgagttgc cgattttcca caatatcaat cagttgatgg gtcttgccgt ccatcatgac
     7981 aaagctcatg gctcccgaga catttttcac tgatttgaat tcatcgaaac acaccacttc
     8041 tggtaacagc tggctcaaag aaatggtttt aggccgaagg ctgcgaagta ttcgaatgac
     8101 agtggtcgga gaaatgtgct tcatacgtgc gatcaaagac atagaaagag gttcgcttaa
     8161 tagctcaaga atggcttgtt tgactctgcg agcaatggag cagcgacgat cactaactga
     8221 atcttctgct aaaaaagtct ttccacagtg tttacatttg aagcgctgtt tgttaatccg
     8281 cagaatggtc ttgtagttac tgacatcatt caataagatg gatacttttt tccagcccta
     8341 tttaatgatc gtttgttcat tttgatagtg gcgatgaaag cattcctccg gttgataggt
     8401 caatgtgcca tcaaaaacaa aacatgtttc gtcattgatt cgctctttgc gaaaggcatc
     8461 ttcatgaaag ataagattta ggtctagaat atctagtaac tctttggtaa actctgaata
     8521 ggacatcttg aaacactcct ttgaatggtt ttcgtcgact ttattctaaa ggattttaag
     8581 gtgtctgttt gcgtttatta gaaaaaagaa aaagtattgg caacagaagg cgtcaccaac
     8641 actagatatt atagatccct tattttcttc gcttcaaatt gtcaaggagt catcataaat
     8701 tttgtatgga ttcttttgtt tgggtagtag tcttctaaat aatcttggat taacaccttc
     8761 atctctattt tacttttata tttgttggag tcgattaatt ctcatttgat tgatcgatga
     8821 aacgcttcaa ttactgcatt gtcaacagga gtcccagttt ggctcactga atgtgtgatg
     8881 tggtggtcgt tcaacagctc ttgataaata aaagaccgat gctggctacc tctatcggaa
     8941 tgaattaccg taggcaattc tccttaataa atttgtaatg gacggacgac aatctctttt
     9001 ttcatgtgtg aatctaccat atagtaacgt agaatacgag ttgctaagtc aatataggtg
     9061 gacaaataga ggcgaccgtc attacatgga atgtaagtaa tatccatcac ccaaacagca
     9121 tcaatttggt tctgttcaaa ttatcgattc aacatttttt taccatttct ttgattgttt
     9181 tttctctacc atgagaagat ttgcgtcgat gaaatcctct tgtgcataaa ttcatttcac
     9241 gcataagaac agctacaaca cgtttattga tttcgattcc ttcatcgtaa agtttttgag
     9301 tgatccctgg agatccgtat cgatctttgt gttcgtagaa cactcgtctt cctcttttag
     9361 tcgtttattt tcctgttcaa gaatgtagaa atctgcttta tcaggtaatc tttttccttg
     9421 tccagcaaaa gcatttttcc cgtatcgacg atattctgaa acccatcggg aaattgttgg
     9481 acgagagata ttgtattctt cagcaatttt cttggtagtt acttttttct caaggatatc
     9541 ctgtgaaatc tttagtttaa atttacgatc atatgttttt ctcaatagag ttcattcctt
     9601 ccggtaacgt aactgatagt caagaaaagt gtatcatagc ccaacttagt atcagggttt
     9661 gtcaactaaa ctgtggaagt tgatagataa gagtttttaa caaccaaaat tctcttgact
     9721 atttttgaaa tcagacaaat tttgattgga cacatagttg aataaatcta ccgtcgttac
     9781 ggaaggtaaa tcgcatgctt ttcaattcta gaggagaagt cctcgttgaa gtaccatagc
     9841 ccagttctgg attcgacctc ctgcccactt catacgtaaa taaagtaatt ttaggagtga
     9901 gactgtgaca aaagttattt ttaagctaaa aacgaattaa aaaacgttga taattaaaat
     9961 cattcgtttt aga
//
LOCUS       DAZUMO010000091         9955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000091 DAZUMO010000000
VERSION     DAZUMO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.091"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..439)
                     /locus_tag="ACG8ER_002479"
     CDS             complement(<1..439)
                     /locus_tag="ACG8ER_002479"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5051010.1"
                     /translation="MEEFGIDSISRTEALDFQADYEYELDQITMIPEIHQLLLALHKN
                     RIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGAAENSYEGNVKNLQSY
                     QNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRHK"
     gene            complement(662..1429)
                     /locus_tag="ACG8ER_002480"
     CDS             complement(662..1429)
                     /locus_tag="ACG8ER_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5051011.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1917..2342
                     /gene="lacA"
                     /locus_tag="ACG8ER_002481"
     CDS             1917..2342
                     /gene="lacA"
                     /locus_tag="ACG8ER_002481"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFS5051012.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2359..2874
                     /gene="lacB"
                     /locus_tag="ACG8ER_002482"
     CDS             2359..2874
                     /gene="lacB"
                     /locus_tag="ACG8ER_002482"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFS5051013.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2885..3817
                     /gene="lacC"
                     /locus_tag="ACG8ER_002483"
     CDS             2885..3817
                     /gene="lacC"
                     /locus_tag="ACG8ER_002483"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFS5051014.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3820..4800
                     /gene="lacD"
                     /locus_tag="ACG8ER_002484"
     CDS             3820..4800
                     /gene="lacD"
                     /locus_tag="ACG8ER_002484"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5051015.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4828..5145
                     /locus_tag="ACG8ER_002485"
     CDS             4828..5145
                     /locus_tag="ACG8ER_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5051016.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5145..6851
                     /locus_tag="ACG8ER_002486"
     CDS             5145..6851
                     /locus_tag="ACG8ER_002486"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5051017.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6993..8399
                     /gene="lacG"
                     /locus_tag="ACG8ER_002487"
     CDS             6993..8399
                     /gene="lacG"
                     /locus_tag="ACG8ER_002487"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5051018.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8422..8694
                     /locus_tag="ACG8ER_002488"
     CDS             8422..8694
                     /locus_tag="ACG8ER_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFS5051019.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            8715..9614
                     /locus_tag="ACG8ER_002489"
     CDS             8715..9614
                     /locus_tag="ACG8ER_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5051020.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3138 a         1678 c         1996 g         3143 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaagtgt tgctttttgt
     1801 ttgaatgctt gatattatat aaacaaagaa atgatgaaaa cgttttttcg aacattttgc
     1861 aaaatgtttt ctacttctac gtagcatttc tttctaaaat ttaggaggtg gtcaaaatgg
     1921 ctattgttgt tggtgcagat ctcaaaggta cgagattaaa agatgttgtg aaaaattttc
     1981 ttgtagaaga aggttttgag gtaattgatg tgactaagga cggacaagat tttgttgatg
     2041 ttaccttggc agttgcctct gaagtaaata aagatgagca aaacttaggt attgtaattg
     2101 atgcttatgg agctggtcca ttcatggtag caactaaaat taaaggtatg gttgcagcag
     2161 aagtttcaga tgaacgctca gcttatatga cacgtggaca taacaattca cgaatgatta
     2221 cggttggcgc tgaaatcgtc ggtgatgagc ttgctaaaaa catcgctaaa ggtttcgtca
     2281 atggtaaata tgatggcgga cgtcatcaaa tccgagtaga tatgcttaac aaaatgtgtt
     2341 aaggaaagga gttaaggaat gagaatcgca attggatgtg accacattgt cacagatgaa
     2401 aaaatggcag tatcagaatt cttgaaatca aaaggacatg aggtccttga tttcgggaca
     2461 tatgaccacg cacgtactca ctaccctatc tatggtaaaa aagtcggtga agcagtagta
     2521 agtggtcaag cagacttggg agtatgtatc tgtggtacag gtgtcggcat taacaatgca
     2581 gtcaataaag ttccaggtgt tcgctcagcg ttggttcgtg atatgacatc agctatatat
     2641 gcaaaagaag agttaaatgc gaatgttatc ggtttcggtg gaaagattac tggtggtctt
     2701 cttatgaatg acatcattga agctttcatt gaagctgagt acaaaccaac agaagaaaat
     2761 aaaaaattga ttgcaaaaat cgaacacgtt gaaacacata atgcacatca agcagatgaa
     2821 gatttcttta cagaattcct tgaaagatgg gaccgtggtg actaccacga ttaaggtata
     2881 acaaatgatt ctgacagtca cactaaatcc ttcagtagac atctcttatc ctctagaaac
     2941 attgaaaatt gatacagtga atagggtgaa agatgttagt aagacagctg gtggaaaagg
     3001 cttaaatgtt actagagtct tgtatgaatc tggtgataaa gttactgcta caggtttctt
     3061 aggtggtaaa attggtgaat ttattgaaag cgaattagaa caatctcctg ttagtccagc
     3121 tttttataaa atctcaggaa acactagaaa ctgtattgct atcttacacg agggtaacca
     3181 aacagaaatt ttggaacaag gaccaacagt ttctcatgaa gaagcagaag gttttcttga
     3241 ccactatagc aatcttatta aacaatcaga agttgtgaca atttcaggaa gtttaccttc
     3301 aggacttccc aacgattatt atgaaaaact tatccaactt gcttcggatg aaggagtagc
     3361 agtcgttttg gattgttcag gtgcacctct tgaaacagtt ctaaaatcgt cggctaaacc
     3421 tactgccatc aaacctaata atgaggaact ttcacagttg cttggaaaag aagtaacaaa
     3481 agatattgaa gaactcaaag acgttcttaa agagtcactt tttagtggta tcgaatggat
     3541 agtggtctca ttaggtcgaa acggtgcctt tgcaaaacat ggtgatgttt tctataaggt
     3601 agatattcct gatatcccag ttgttaatcc agtcggatca ggggactcaa cggtagctgg
     3661 tattgcatca gctttaaata gtaaaaaaag tgacgctgac ttattaaaac atgcgatgac
     3721 attgggtatg ttaaatgctc aagaaacaat gacagggcat gtgaatatga ctaattacga
     3781 aacactaaac tcacaaattg gagtaaaaga ggtataaaaa tggtacttac agaacagaaa
     3841 cgcaaatctt tggaaaaaat ttcagataaa aacggtgtta tctcagcttt ggcatttgac
     3901 caacgtggtg ctttgaaacg tttgatggca cagtatcaag atacagaacc aactgtagct
     3961 caaatggaag aactaaaggt tttggttgct gatgaactta caaaatacgc ttcttcaatg
     4021 ttgcttgacc ctgaatatgg acttccagca acaaaagcat tggataaaga agcaggtctt
     4081 cttcttgcct atgaaaaaac aggttatgat acatcaagta caaaacgttt gcctgactgc
     4141 ttggatgttt ggtctgcaaa acgtatcaaa gagcaaggcg ctgatgccgt taagttcttg
     4201 ctttactatg atgtagacag ctcagatgaa cttaaccaac aaaaacaagc ttatattgaa
     4261 cgtgttggtt ctgaatgtgt cgctgaagat attcctttct tcttggaaat ccttgcttat
     4321 gatgaagaaa tctcagatgc aggttctgtt gaatatgcaa aagtgaaacc tcgcaaagtc
     4381 attgaagcga tgaaagtttt ctctgatcca cgctttaaca ttgatgttct taaagtagaa
     4441 gtaccagtta acgttaaata tgtcgaaggt tttgctgatg gtgaagtggt ttacagtaaa
     4501 gctgaagctg ctgacttctt taaagcacaa gaagaagcga ctaaccttcc atacatctac
     4561 ctaagtgcag gtgtatctgc taaattgttc caagaaacat tgcaatttgc tcacgactca
     4621 ggtgccaagt ttaatggtgt gctttgtgga cgtgctactt gggcaggatc tgtagaacct
     4681 tacatcaaag aaggtgaaaa agcagcacgt gaatggttgc gtactactgg attcgaaaat
     4741 attgatgaac ttaacaaagt tcttgttaaa acagctagtc catggactga taaagtatag
     4801 taaaaacata aaacggagga tattgttatg aacagagaag agatgactct cttagggttt
     4861 gaaattgttg cttatgctgg agatgctcgc tctaaacttt tagaagcgct taaagcggct
     4921 gaaaatggtg atttcgctaa ggcagatagt cttgtagtag aagcaggaag ctgtattgca
     4981 gaggctcaca attctcagac agctatgttg gctcgagaag cttctgggga ggaacttcca
     5041 tacagcgtta ccatgatgca tggtcaggac cacttaatga ctactgtctt attaaaagat
     5101 gtgattcatc acctcatcga actttataaa agaggagcaa aataatgcat aaactcattg
     5161 aacttattga gaaagggaaa ccgttctttg agaaaatctc tcgaaacatc tatcttcgtg
     5221 ctattcgtga tggatttatc gctggtatgc cagtcatcct tttctcatct atctttatcc
     5281 ttattgccta tgtcccaaat gcttggggat tccactggtc aaaagacatt gagaccttct
     5341 tgatgactcc ttatagctat tcgatgggta tcctagcatt ctttgttggt ggtactaccg
     5401 ctaaagcttt gacggactca atgaaccgtg acctacctgc gaccaatcag attaacttct
     5461 tatctacgat gctagcgtct atggtagggt tccttttgat ggcagctgag cctgcaaaag
     5521 aaggaggctt cttgacagcc ttcacgggaa caaaaggtct tttgacagca tttattgcag
     5581 cctttgtgac agttaatgtt tacaaggtct gtgtgaaaaa taatgttact attcgtatgc
     5641 ctgaagaggt tccaccaaat atctctcaag tatttaaaga cttgattcca ttcactgtag
     5701 cggttgttct cctttatgga tttgaactca ttgttaaggg aactcttggt gtaaccgttg
     5761 cagaatcaat cggtaccgtt cttgctcctc tcttctcagc tgcagatggt taccttggaa
     5821 ttacacttat ctttggtgct tatgccttct tctggtttgt tggtatccac ggtccttcaa
     5881 ttgtcgaacc agcaatcgct gctatcactt atgccaatat cgatgccaac ttgcatctta
     5941 tccaagctgg acaacatgca gataaagtta tcacttctgg tactcaaatg tttattgtta
     6001 ccatgggtgg aacaggagct acattgattg ttccattctt gttcatgtgg atttgtaaat
     6061 cagaacgtaa ccgtgccatc ggacgtgcct cagttgttcc aacattcttt ggggttaatg
     6121 agccaatctt atttggtgca ccaatcgtat tgaatccgat tttctttgta ccgttcattt
     6181 tcgctccgat tgtcaacgtt tggatcttta aattctttgt tgacacattg aacatgaact
     6241 cattctctgc caaccttcca tgggttactc ctggtccgtt gggaattgta ctcggtacta
     6301 acttccaagt tctatcattt atcttagccg gactcttggt agttgttgat actatcattt
     6361 attacccatt tgttaaggta tacgatgaac aaattcttga agaagaacgt tctggtaaaa
     6421 caaacgatgc tcttaaagaa aaagtagctg caaacttcaa tactgctaaa gcagatgccg
     6481 ttcttggaaa agcaggcgtt gctaaagaag atgtagcagc aaacaataat atcacaaaag
     6541 aaactaatgt ccttgttctt tgtgcaggtg gaggtacaag tggcttgctt gccaatgcct
     6601 tgaacaaagc agcagcagaa tacaatgttc cagtcaaagc ggcagcgggt agttacggtg
     6661 ctcaccgtga aatgttgcca gagtttgact tggttatctt ggctccacag gtcgcttcaa
     6721 acttcgatga tatgaaggct gaaacggata aattgggcat taaacttgca aaaactgaag
     6781 gagctcaata tatcaaattg acacgtgatg gacaaggtgc tcttgcattt gttcaacaac
     6841 agtttgatta ataaatttgt aaaagttatt tgagacgaga ttattctttc agttccttat
     6901 aatattgtta accatatcat tgcatttcat ttccttggtt tccggaactc agaatatcgt
     6961 ctcaaatgct ttttttgaaa ggatttatag ttatgactaa aacacttcct aaagatttta
     7021 tttttggtgg tgctacagct gcttatcaag cagaaggtgc gactcacacg gatggtaaag
     7081 gcccagttgc atgggataag tatcttgaag acaattattg gtacacagca gagcctgcaa
     7141 gtgattttta ccataaatat cctgttgatt tagaattagc tgaaaaatat ggcgttaatg
     7201 gtattcgtat ttcaattgcc tggtctcgta ttttcccaac tggctatggc gaagtaaatg
     7261 aaaaaggtgt tgaattctac cataatcttt ttgccgagtg ccacaaacgt catgttgagc
     7321 cattcgtgac tttgcaccac tttgacacac cagaagttct tcactcagat ggagatttct
     7381 taaaccgcga aaatatagaa cactttgtag attatgccgc tttctgtttt gaagagttcc
     7441 cagaagtaaa ctactggaca actttcaatg aaattggccc aattggagat ggtcaatact
     7501 tggttggtaa attccctcca ggcattcaat atgatcttgc caaagtcttc caatcacacc
     7561 acaatatgat ggtttctcat gcacgtgctg tgaaattgta taaagataaa ggttataaag
     7621 gcgaaattgg tgtagtacat gcgttgccaa ccaaataccc ttatgatcca gaaaatccag
     7681 cagatgttcg tgccgctgag ttagaagata tcatccataa caaatttatc ttggatgcaa
     7741 cctaccttgg acactattca gataaaacga tggaaggggt caaccacatc ctagctgaaa
     7801 atggtggaga acttgatctt cgtgatgaag acttccaagc acttgaagca gctaaagatt
     7861 tgaacgattt ccttggtatc aactactaca tgagtgattg gatgcaagcc tttgatggtg
     7921 agactgaaat cattcacaat ggtaagggtg aaaaaggaag ttctaagtac cagattaaag
     7981 gtgttggtcg ccgaatcgca ccagattatg tgcctagaac ggattgggac tggattattt
     8041 atcctgaagg cttgtatgac caaatcatgc gagtgaaaaa tgattatcca aactacaaga
     8101 agatttacat cactgaaaac ggtcttggat acaaagatga gtttgtagat aataccgttt
     8161 atgatgatgg tcgtattgat tacgtgaaga aacacttgga agttttatca gacgcaattg
     8221 cggatggtgc aaatgttaaa ggttacttca tctggtcact tatggatgtc ttctcatggt
     8281 caaacggata cgaaaaacgt tatggactat tctatgtaga ctttgacacg caagaacgct
     8341 atcctaagaa atcagctcat tggtataaga aagtagcaga aactcaagtg atagagtaat
     8401 attacttgaa aatgtaaaaa attgttatcc gatactttag aaaaagtaca acacttattt
     8461 agaccaggct ttgaaaaggt atatattgtt agtttcgaag attgtcctat gattcctgag
     8521 cttgaagcta ccccgttgct aaaatgtggt aaatggtatg tttcaactgg aaaagagtgg
     8581 atttgccatt ctgatttaga gttatcagca tttgaatggg aatttttaca atcactcgat
     8641 gtagaaatac gtgagactat tcacttcgag gtaaattatt tgcctttcca ataataaaaa
     8701 gaaaggaatt agttgtgact atcgaattaa aaaacgagta tcttactgtt cagtttaaaa
     8761 cacttggtgg tcaactgact tcgattaaag acaaggatgg gatagagtat ctctggcaag
     8821 ctgatccaaa ttattggaat ggacaggcgc ctattctatt tcctatctgt ggtagtttac
     8881 gaaatgattg ggctatttat agacctcaag aaagaccttt ttttacaggt cttattcgta
     8941 gacatggttt tgttcgaaaa gaagaattta ttctggaaga agtcaatgat aatagcgtga
     9001 cctttagtat taaaccaaat gctgagatgc ttgataatta cctatatcag tttgaactga
     9061 gagttgttta taccttgaat ggtaaatcaa ttagaaccga atttcaggta acgaacttgg
     9121 aaatggaaaa aacaatgcct tactttattg gagcccatcc agcattcaat agtcctttag
     9181 tagaaggtga aaactatgag gattattata ttgaatttag tgaggttgag tcttgctcga
     9241 taccaaagag ttttcctgag acaggattat tagatttgca agatcggaca ccttttttgg
     9301 aaaatcaaaa aattctcgat ttggactact cacttttttc ccatgatgcc attacacttg
     9361 accagctcaa gtctcgtagc gtaacgcttc ggtcaaggaa gtctggtaaa ggtcttcgag
     9421 tagattttga tgattttccg aacttgattc tgtggtctac aactaataaa agtccattca
     9481 ttgctttgga accttggagt ggtctctcca cttcacttga agaaggtaat ttcttagaag
     9541 ataaaagaca ggtaacgaaa atttcacctc aagaaacttc tagaaaaatc tatgatatta
     9601 ctatccttaa ttaaaatttg ttattatttg agtcttcaat aagatttgaa tactcttacc
     9661 ttgtgatgat agattggtct tctccgtgcc agtcttacat cgcaaggttt ttaagttttt
     9721 tgtgggtcta tgtcaactgt agtgggtgac gaaaagctaa tatctaaaga cagtctggta
     9781 gtaaaaaata agattattta ctacttcttg atttaaaata atagtggagc cattaatttg
     9841 tggcaaattt aatttaatat tttgcctcaa attagccaca ctttttatag aactaagagg
     9901 aaaaatgaaa ttttcttcct attaatatag ttgtttgaaa tcttaaatat agcgt
//
LOCUS       DAZUMO010000092         9887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000092 DAZUMO010000000
VERSION     DAZUMO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.092"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..221)
                     /locus_tag="ACG8ER_002490"
     CDS             complement(<1..221)
                     /locus_tag="ACG8ER_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5051021.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEK"
     gene            422..1636
                     /locus_tag="ACG8ER_002491"
                     /pseudo
     CDS             422..1636
                     /locus_tag="ACG8ER_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1815..2150)
                     /locus_tag="ACG8ER_002492"
     CDS             complement(1815..2150)
                     /locus_tag="ACG8ER_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5051022.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2134..2397)
                     /locus_tag="ACG8ER_002493"
     CDS             complement(2134..2397)
                     /locus_tag="ACG8ER_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5051023.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2857..3660)
                     /locus_tag="ACG8ER_002494"
     CDS             complement(2857..3660)
                     /locus_tag="ACG8ER_002494"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5051024.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3638..4762)
                     /locus_tag="ACG8ER_002495"
     CDS             complement(3638..4762)
                     /locus_tag="ACG8ER_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5051025.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4778..5593)
                     /locus_tag="ACG8ER_002496"
     CDS             complement(4778..5593)
                     /locus_tag="ACG8ER_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5051026.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5583..6455)
                     /locus_tag="ACG8ER_002497"
     CDS             complement(5583..6455)
                     /locus_tag="ACG8ER_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5051027.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6540..7829)
                     /locus_tag="ACG8ER_002498"
     CDS             complement(6540..7829)
                     /locus_tag="ACG8ER_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5051028.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7973..8485)
                     /locus_tag="ACG8ER_002499"
     CDS             complement(7973..8485)
                     /locus_tag="ACG8ER_002499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051029.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8552..8695)
                     /locus_tag="ACG8ER_002500"
     CDS             complement(8552..8695)
                     /locus_tag="ACG8ER_002500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5051030.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(9178..>9887)
                     /locus_tag="ACG8ER_002501"
     CDS             complement(9178..>9887)
                     /locus_tag="ACG8ER_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFS5051031.1"
                     /translation="LVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRP
                     KGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQC
                     TISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWT
                     KYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLE
                     QLLSTVWLSVRTAFFVG"
BASE COUNT         3043 a         2092 c         1594 g         3158 t
ORIGIN      
        1 aacttttctt tgactttcaa tatctgagtg cgtgtcgtat aggaaccgtt gcggataata
       61 cgaaccgatc gacagtgttc acaataggaa ggagagtcat ccaattttcc tttaataaac
      121 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
      181 ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag
      241 gatgacgttt tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa
      301 gaaaaagtgt tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaagc
      361 aaaatcaatt ctaccttaga tgttcttata tcactctatc agtcctattt gacaaagagc
      421 caagaaaaag acgttggtcg tcgatgctgt tctctcgcct actcctcgtg cttgtagaag
      481 ttgtggttct actgtggtag atggaaacgg gaaagcaatt atagtgaaaa atgggaagaa
      541 ggaaacgatt gtccgttttg aacaatacaa tcatatgcct ttggttacgc gcctaaaaaa
      601 gcaacgctat acctgtaaaa actgtcgtac ccattggacg gctcaaagtt attttgtccg
      661 actcagccat tcaatcgcag atcatgttcg atataaaatt gcttctttac tgactgaaaa
      721 agtatctttg tcttttattg cgaaaagttg tcaggtatct ttgactaccg ttactcgtac
      781 attgaaagag tttaaaagct atttaccaaa gccttctaag aagattctcc ccagagtatt
      841 gatggttgat gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc
      901 agatggtgaa acaggaaact taattgctgt cttgcctacg cgtaaattac ctcgattaac
      961 aagctatttc ttaggctgca ccaatccaga agaagtagaa ttcttagtca cagacatgaa
     1021 cgccgcttac ttccagctta ccaaacgtgt tctgccaaat gcgaaagtag tgattgatcg
     1081 gtttcatatt gtcaaacaca tgaatcaggc actcaatgag ttgcgtatcc gtgaaatgaa
     1141 tgagcttcgt aaagcaggac agaaaagtca ggcagaaaaa ctgaaaaaga actggcgctt
     1201 tctgctaaaa aatcgtgcaa gcatcaacca ttatgagtac aaaacatgga aaagtttccg
     1261 agcaccaaaa tacccatttc tcactgaagc aatgatgatt gatcgattgc ttgagttttc
     1321 tgcgcctcta aaggaggcgt atcccttttt tcatgaatta gtcgaagcct ttcgagacaa
     1381 agaccctgac ttatttttct ccttattggc agaacttccc gaaacgttag atgacggctt
     1441 tcgggagaag cttcaaaacc ttctgaccta tgaagaaggt atcaccagcg caatgatcta
     1501 tccttattcc aatggaaaaa ttgaagcgaa gaatacccac ataaagacaa tgaaacgagt
     1561 atcctacgga tttaaatcat tcgagaacat gagaattaga atctttttga tcaatcaatt
     1621 aatcaatgta agataacaaa aaatctgaac cagaatgtgg ttcattctga cccggatttg
     1681 attttgcaca actcatcagt ccttattgac aaagagccaa aaatctgagc cagaatgagg
     1741 ttcattctga cccagatttg attttgtaca acccatcagt ccttattgac aaagagccct
     1801 tctttttagt attattataa ttcttccatg cgttcttcca gtcgctgtaa atcgcttgga
     1861 cttaattccc caattttttt ccatttgacc tttttgttgt ttttgggtaa ttcgtttaga
     1921 gttcctacgt caatccaaga ttcttttttt agtcctgcct gtttccaatc ccttatttca
     1981 acgtatttca gctttatttt atcggattta gtatgatatt ttgaagtaat tgacagaaac
     2041 gaaattgtgt ccagtccatt ttttagaata tagacaggcc ggcgtttacc gccctctgca
     2101 gctgtgaaag caacgtagac ggttgctata tcattagtat tcatcttcat tgaaccattc
     2161 ttcaattttt tcgtcagtgt ctagtttttg gacaggcaat ttttccgcta atctttgaat
     2221 attatggatt cgtttacttt tttccgacaa ttcaatagaa aatgggattt ttccttgctc
     2281 aacaatttgc ttataaaaaa cggtgacggc tgttttgggg ctcatgccta gttcatcaat
     2341 aatgctttct gcttcatttt tcaatttttc gtccatagaa atttgtaact tagccatttt
     2401 ctccacctct ttctgacaaa cacattatat cacggaaaga gttcaaatgg aattaataca
     2461 actacttttg aactccttga aatgcagtgt atttatctaa atataaggaa aggatatagt
     2521 gctaaatggc cttgatagat agaaaagttt caaaatccgc cacagcgata attgaagcga
     2581 acactcaacg acacacagaa caggaaaaga ctcctaaaag aaccaaatta actaggacgt
     2641 gtctgaaaac tccacaaaga tgtggtatgc tctggtaaaa aacgaatggc acaaccacga
     2701 agaccgatat ggttcttgga aaactttctc cacccttttt gtaattggag ggatagtggt
     2761 ttattgatag cgattttcca agttcttcaa gtggaaccag acattgagca ccacggctaa
     2821 aaagtgaatg aagtctttat ttttaatcaa taatttttaa tgattaccag taattggttt
     2881 tttcatgcgc cctatttcta tttgatgtgg agttaaataa tgctgataag taaaaatttt
     2941 tccgtcaatt aaactgccac tagtgaatcc tgttctattg gtaaaaagag aatagtctgg
     3001 gtattcatct acaccaaatg ataaagattc agccataaac tgttttttac cagcaaattc
     3061 agcactgcta ccttgatgga tatgtcctac aaaaatacct aaaatatcac tttcggcaat
     3121 caattcagca aaaccttccg gaacttcagt tacaatagat ggatcttccc acgacaatgg
     3181 atgatgaacc aataaaatcg ttcccttttc agcaggtgta cccaaccact cttgtaattg
     3241 aatcatctgt cctatcgata tttttccttc gtgctgggta tcttgagcag aatccaagga
     3301 aataattcgt aacccatttt gattcgtaca atggatatac tctcccgttt cattgctttc
     3361 aacactcatt ccttcaaaga aaacactgcg acgatcatga ttcccacgac aaaaatagta
     3421 aggtgtggtt gggaaatatt gatccaacat tttccgtaag acttgataat cagaaatccc
     3481 cccttcatgt actaaatcac ctgttaatag aataaaatcg tacactgtta acgagagttc
     3541 ttgtaacaat ttttccaatg ccaactgtgg atttttaatt gttggaaata gttcagcagt
     3601 aaacaagtta gtttgatagt tttctctaaa atggatatca gagagatgca aaaagcgcat
     3661 ggctattccc tctttcttca ataaaataat caggatatcc taaattttct tgcgtctgat
     3721 aatcaaaaaa atgcattctt tcaggttcta tgcggagatt gactgcatca ccaatctgcc
     3781 aagtaggttt ttcattaagg acaatcattt ctataccatc gactttaacg gtgtacgcat
     3841 aactttgacc taatagctca cgatacttca cttgaccgtg taaatcgctt gtttcttttg
     3901 aaaaggttac gtgttctggt cttatgccta aatacaatga gccactaacc tgaagaagtt
     3961 gtttttgcca agctggattc aatgttaaaa attgactggc tacctttaat gttccttgat
     4021 cataagtaac tggcataata ttcatgcctg gcgaaccaat aaaatttgca gtaaaagtat
     4081 ttttaggttt cgtataaaca tttttcggcg tatcaatcat ctgaattcta ccttcattca
     4141 ataaaacaat ccggtcagct aacgtcatcg cttcaatttg atcatgagtg acgtagacaa
     4201 aagtttgttt ataacgttga tgaattttta ccaattcttt tcgtgcatcc aatcgtaaac
     4261 gcacatccaa atttgataac ggttcatcta ataaaaaata attactccgt ttaaccaccg
     4321 cacgagccaa tgctacacgc tgccgttgcc ctcctgacaa atctttagga agtcgatctt
     4381 ggtatggcgt taaacctaac atcttcaaag cttcttccaa acggtgctga atgtcagctg
     4441 tcttaatttt attcgccctt aatgcaaaaa taatattctc ctttacagtc atgtgaggat
     4501 ataaagcata attttgaaaa accattgcga tctctcgttc tccacaaggt agcttatttg
     4561 ctagttgtgt acctaaaaaa agctctccac tcgtaatttt ttccaaacca gcaatcattc
     4621 tcagcaaagt agattttcct gaaccagatg gtcccagtaa cacaattcgt tctccagcgg
     4681 ccacagctaa atcaatttct tttaaaatgg tatgttctcc atattttttt ccgatatttc
     4741 ttaattcaag cgtttctccc atgatttgtc ctccaattta tttaatacct gcagaaacaa
     4801 aagtcccgat aatatatttt tgaaaaattg catacaagac taacggtaca aacattgtaa
     4861 ttaaactaat cgccattgct acggtaaaat ttgtcccacc ttcagaacta atataggtct
     4921 gcatcgccaa cggcaaagta taattatcta ctgtccgtaa aattaatacc ggccaaacat
     4981 actcattcca agaggtaata aaaaaccaaa taccggtaga agtaatcgca tgtctagtaa
     5041 gaggaaaaac aattttactc atgacggttg tttgtttgat gttatccaat gccgccacat
     5101 ctagaaggct cactggaatt tcccgcattg tttgcgtcaa taaaaaaatg cccatcgcat
     5161 cagcaaattg tggtattatc acaccccaag tagtatcaat taaattaagt tttgcaatca
     5221 acaaataatt aggaatcatc gcaacagtaa aaggaatgaa catcgtacca atgatgataa
     5281 agtaccagat tttcttccct ttgattcggc aataagtaaa actatatgcc gcaaaaaaag
     5341 ctacgcctaa cttaacaacc gttataattg tagcaataaa aaaagtattt ctcgtaattt
     5401 tgataattgg aagattttcg aacaaatagc aaaaattttc caaggtaaac ggctgtggta
     5461 tcaaactagt aatcgtctga taggcatctt tcaatggctt aaaagaattg gccataataa
     5521 acaataatgg atagataata atgattatga tgaacataaa taagagatgt ggcaaccagc
     5581 gtttaatttt catagtaaac ccccttttcc acaaatttaa atattacccc taagaaaaca
     5641 gcaaatatta ataaagttaa aactgaaatt gctgaagatt ctccgacttt atacatttca
     5701 aaagcctttt gataagcata atagatcacg tttgcactac cattattcgg tccaccacga
     5761 gtgatgatct taatcggagt aaacacatac tgcagacctt gaacaatggt catcaccaat
     5821 aaatatagtc ctgtcgaact actcataggc aacacaattg taaaaaaaat gcgccagatg
     5881 ggtacacgat ccaacttagc tgcttcgatc atctcttgat caattcccac aattgctgca
     5941 aaaatgacaa tgaaattata accaaaaact ttccaagcag taatacctgc aatcacagca
     6001 attacccaac cttgtaaatt catccacata ggcagtctta aaccaaaatt tcccaacaag
     6061 acagcaattg gtcccgatac cggatttaat atccatgtaa aaaccatccc cgccactacc
     6121 aaagaaatca cagctggaat aaaaaataat ggtttataca aattctgccc tttttctata
     6181 actattccta ataaaaagga tgtaatatat gggaacacac agctcagcaa gacaaataga
     6241 acggtatagg acaaagtatt cttcaacact ttatgaaaga ctggatcagt cagcaccttt
     6301 acatagttgg caaagccaac aaattcttta gtgggggcca ccatattcca gtcaaaaaaa
     6361 ctcaaataga tcgtataaat caatggataa aaaacaaaca cgcccaacaa aataattgct
     6421 ggcgccagat acaaataacc tacatagcgc ttcatttccg acagcctcct catccttatg
     6481 tcaaaagcag acgaccttgt ttttcctttc ttacaggcgc tgtcgtctgc ttgctttgct
     6541 tatttgagta actcattaat ttccttttga gtagctttca tcgtttcatt gacggaatta
     6601 cctgataaaa ttttatcccg catatccaac aatttttgtt cagcttctaa tccagcatct
     6661 ccaggaaatg aactccaact gaccatctta tccatctgat caattgctgg tatcatcatc
     6721 tgattttcag ctaagaaagc ttttaatcca ttttgggcgt ctgcaacatc tttacgtggt
     6781 ggtaaataac cagtaccttc tgtccattta gctaaagatt caacactata aagatatttt
     6841 aagaaatccc aagttgcttc ctttttctta tccgtttgtg atgtaacagc taacattgcg
     6901 ccaccagcag gtagtttaac ttttttccct tcccaacttg gtgactcaac tgctttagca
     6961 gtaaaagtac tatttttttg aatgttgtcc ctttgagcaa tagtggtata gaccatcgcg
     7021 acgttaccat caataaagct ttgcacacct tgttcccacg tagtgtgtaa tgccgtctgg
     7081 tcttttacca tcctttgata taattcataa gcagcgatac catctttaga agcaaaggta
     7141 gctttgccat cttttattac acttgcccca ttactttcta aaattgcttg ttgtgcccaa
     7201 ttatcagcag gttcttgaat atacacgcca tactttccag ttttttcttt tattatctta
     7261 gaaaaagttt gaacttcttc ccaagttttt ggaccatttt catccaagcc cgctgctttt
     7321 aatatatctt gatttaaata taaaaccgga ttactaatgg agtaaggaat acccacttgt
     7381 ttaccatctt tttcagacag tgccatgata ttgtctaaaa aatgatcttt aaaataatca
     7441 gcattatccg gatccacttc ttctactaaa gattgaggat tagtatattt gaaattttct
     7501 gcaaaataat ctttaaaagc ccatcccact tgtactacgt ccggtatttc tttggaggca
     7561 acagaagctt gtaaattttg catcaagcct ttatacatat caggattata ttttgctaca
     7621 accttcactt tgtcttgaga ttgattgtat ttatctacta attcattcac tgtttttcca
     7681 cctagggtat ccgcattaac atgccaatac tctatcgtta cacgaccctc cgattttttt
     7741 gttgtcttgg tattcccact accacaagct gccaaagaga tgacacttaa aacagacaca
     7801 acccctataa caagattttt ctttaacata acattaactt cctcctaatt tctttatggc
     7861 catttttcta atcaagaaat attatgaact accaatgtaa atttctagtt aattttctgt
     7921 aaataattcg aaatttgtgc atttcggggt acagggtcac tggacatatc catcaagcta
     7981 agaataaagc ccccatatgg tcatgtatcc catctccaaa tctcttaact cctctgtttt
     8041 gattataaaa tagtttattc tgtgctcctt tcttccataa ataggcccat gcacaacgtg
     8101 aggttatatt ataaacacca cagcgcgcaa ggtactcaat cgtttctagt tcattgactg
     8161 tccatacaga aaatgtcaat cctagctgct gccccacttc acgggccaac ttagttaacc
     8221 atccttcatg aatattgacg atttgaacgc tttgataggc taatttctgt aaggtatcga
     8281 tcaaatctgc ttcggtaata tccccaagtt ttttcccttc gaactgattt tcaatattat
     8341 aaaatatatg ctttccccaa atcgcagccc aagataattg tacatagcca ctaagaacaa
     8401 tttgtggcca gatttgatag gtttgtgcca gcgaaaaaac ggccatttcc aatccctctt
     8461 cttttaaatc acagttaatg gtcatggttg ccttctcatt ggccactgct tgaaacacct
     8521 gctcaagggt taccgaatct gggacaactt cttacctgtc atgggctagt attagcgtac
     8581 catctgtttg ctgacgcaca tctacttcaa aacaaagctg gcgatccaaa caaaagcgaa
     8641 gaaatgccca gctattctcc tccgtattgt cactaccaga gtgtgcagtg atcatggtca
     8701 ttttgacccc tcctttaatt ccgtgatttt ctcccacacg tccactatac cgatgaagcg
     8761 ttaatttctt gttaatttct tgtaaattcc ctgcgttttt tcagtaaact agttgtaaat
     8821 cttgcatgct ccttcctacg aactcatgaa tcgtcgattt tgcccttcta gctagtttca
     8881 ccaattaagt agttctacat tccccgcttt taacctacta cacctttttt tgttttcagc
     8941 tattccttct tcttgctgaa agtccttgag gttctttctt acaggatacc atcaaggtgt
     9001 tcaatggctt attcccattg aatgaccgag acttactatc agatttctgc tcgttgaacc
     9061 aagtctttga tacaggattt cttccgtcat cttctcacaa ataccacctt gcgtttctct
     9121 agcgattggt cgatgtgcac cctcccagtg gactgtcact accccttttt gctatgccta
     9181 gcccacaaaa aaagctgtcc tcacagacag ccagactgta gacaaaagct gttccaaact
     9241 cgtaactcga gcttggaaca gctttttatc tacttcgttt cgaatttttg aacaaaaaaa
     9301 taaaggctcc ttgcctttcc atagccaggt ggctaatttc tttaaattca ttgcagcaaa
     9361 cactagcatc gtatgcgtcg cgactttttc caagcctcga tacttggtcc aacgcatccc
     9421 atgcttttct tttgcatccg caaatattcg ttcaatcgtt tgtttccgtt ttttgtaaat
     9481 tgatttattc agatctgtta ggcgaagatg ttccacttca tccatcagat ctccccaaac
     9541 atgtctcgtg atgactcgtt gatgattttt acttatggta cattggttta gcagaggaca
     9601 gttcacacat tcttttggat tggatttgta ttctcggtac ccttctcgag taaccgttga
     9661 aaaagcgagc atttgatctt gcggacaaat ataacaatca aaatactcgt catagagaaa
     9721 ctcatttttt gataatagcc cctttttccc tttgggcctt gaataaggca aacaaggtcg
     9781 aaggttcaaa tgagtcaaaa agtgaacaaa cttaggtgtc ttatacccag catcagcgac
     9841 aacacaattg atttcaggaa aaagtctctt cgattgtttg acgagct
//
LOCUS       DAZUMO010000093         9805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000093 DAZUMO010000000
VERSION     DAZUMO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.093"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACG8ER_002502"
     CDS             complement(<1..205)
                     /locus_tag="ACG8ER_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051032.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="ACG8ER_002503"
     CDS             complement(221..835)
                     /locus_tag="ACG8ER_002503"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS5051033.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="ACG8ER_002504"
     CDS             complement(982..1665)
                     /locus_tag="ACG8ER_002504"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5051034.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="ACG8ER_002505"
     CDS             complement(1774..2232)
                     /locus_tag="ACG8ER_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS5051035.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="ACG8ER_002506"
     CDS             complement(2316..4079)
                     /locus_tag="ACG8ER_002506"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS5051036.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="ACG8ER_002507"
     CDS             complement(4300..5760)
                     /locus_tag="ACG8ER_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051037.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8ER_002508"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8ER_002508"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS5051038.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="ACG8ER_002509"
     CDS             complement(6917..8344)
                     /locus_tag="ACG8ER_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5051039.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="ACG8ER_002510"
     CDS             complement(8375..9589)
                     /locus_tag="ACG8ER_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS5051040.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2905 a         2040 c         1624 g         3236 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tattttaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat tttta
//
LOCUS       DAZUMO010000094         9689 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000094 DAZUMO010000000
VERSION     DAZUMO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.094"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACG8ER_002511"
     CDS             185..1183
                     /locus_tag="ACG8ER_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5051041.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACG8ER_002512"
     CDS             1295..1804
                     /locus_tag="ACG8ER_002512"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS5051042.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACG8ER_002513"
     CDS             complement(1834..2493)
                     /locus_tag="ACG8ER_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS5051043.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACG8ER_002514"
     CDS             complement(2495..3259)
                     /locus_tag="ACG8ER_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS5051044.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACG8ER_002515"
     CDS             complement(3267..3944)
                     /locus_tag="ACG8ER_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS5051045.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACG8ER_002516"
     CDS             complement(3981..5366)
                     /locus_tag="ACG8ER_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS5051046.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACG8ER_002517"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8ER_002517"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG8ER_002518"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG8ER_002518"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS5051047.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACG8ER_002519"
     CDS             6305..7162
                     /locus_tag="ACG8ER_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051048.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACG8ER_002520"
     CDS             complement(7243..7578)
                     /locus_tag="ACG8ER_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051049.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACG8ER_002521"
     CDS             complement(7645..9015)
                     /locus_tag="ACG8ER_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS5051050.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACG8ER_002522"
     CDS             complement(9093..9629)
                     /locus_tag="ACG8ER_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5051051.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2845 a         1998 c         1621 g         3225 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatggtactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaata
//
LOCUS       DAZUMO010000095         9297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000095 DAZUMO010000000
VERSION     DAZUMO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.095"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACG8ER_002523"
     CDS             complement(78..2198)
                     /locus_tag="ACG8ER_002523"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5051052.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACG8ER_002524"
     CDS             2428..3606
                     /locus_tag="ACG8ER_002524"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS5051053.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8ER_002525"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8ER_002525"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS5051054.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8ER_002526"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8ER_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS5051055.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACG8ER_002527"
     CDS             complement(4959..6212)
                     /locus_tag="ACG8ER_002527"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5051056.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8ER_002528"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8ER_002528"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS5051057.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACG8ER_002529"
     CDS             7789..9135
                     /locus_tag="ACG8ER_002529"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS5051058.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         2995 a         1680 c         1925 g         2697 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatc
//
LOCUS       DAZUMO010000096         9064 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000096 DAZUMO010000000
VERSION     DAZUMO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.096"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..190
                     /locus_tag="ACG8ER_002530"
     CDS             <1..190
                     /locus_tag="ACG8ER_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051059.1"
                     /translation="FTEFVYRHVNLFAEPLFVLYESNSSIVSELANLMIALLFTPALR
                     VITILVCLVLLFFVNKKK"
     gene            complement(374..1123)
                     /locus_tag="ACG8ER_002531"
     CDS             complement(374..1123)
                     /locus_tag="ACG8ER_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5051060.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1309..2013
                     /locus_tag="ACG8ER_002532"
     CDS             1309..2013
                     /locus_tag="ACG8ER_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5051061.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2053..2610
                     /gene="hxlB"
                     /locus_tag="ACG8ER_002533"
     CDS             2053..2610
                     /gene="hxlB"
                     /locus_tag="ACG8ER_002533"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFS5051062.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2618..2926
                     /locus_tag="ACG8ER_002534"
     CDS             2618..2926
                     /locus_tag="ACG8ER_002534"
                     /inference="COORDINATES: protein motif:HMM:NF013403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051063.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     "
     gene            3005..3757
                     /locus_tag="ACG8ER_002535"
     CDS             3005..3757
                     /locus_tag="ACG8ER_002535"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051064.1"
                     /translation="MKKEIYSLLSEKEQENFDEYAYFVNTAIDRQSLGKVLGGKMISV
                     VEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAAHAAVAYLGALKGY
                     KTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKTLNRHGNQKLSDEI
                     TRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYRNLADPEAEQLRRM
                     IVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            3769..4200
                     /locus_tag="ACG8ER_002536"
     CDS             3769..4200
                     /locus_tag="ACG8ER_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5051065.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4214..5659
                     /locus_tag="ACG8ER_002537"
     CDS             4214..5659
                     /locus_tag="ACG8ER_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFS5051066.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            5945..7570
                     /locus_tag="ACG8ER_002538"
     CDS             5945..7570
                     /locus_tag="ACG8ER_002538"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5051067.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            8202..8555
                     /locus_tag="ACG8ER_002539"
     CDS             8202..8555
                     /locus_tag="ACG8ER_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051068.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT         3118 a         1272 c         1776 g         2898 t
ORIGIN      
        1 atttacagaa tttgtttata gacatgtgaa tttatttgca gaacctttat ttgtattata
       61 cgaatcaaat tcctcaatcg tcagtgaact tgctaattta atgatagcat tgctgtttac
      121 tcccgcgttg agagtcataa ctattttggt ctgtttagtg ctattgtttt ttgttaataa
      181 aaagaaataa cgtagtattt caaaagacaa agaagtggga tgatgattgc tatagtatgt
      241 tatacgtggt gctaggtaag tgagaataaa caaaaaagga aacacgcaat aaagtttatt
      301 tcaaaacttt attgcgtgtt tcttttttag attttttata tctgttgctt tagtagttac
      361 caatagtttt cgcttaatca atcaaaacga ttttttgttt attgtattct ttacgaacct
      421 tttcaggaat ttcttctgaa ataagatagg taaggtcttc aattggaccg acgatatgat
      481 tactgatggt gccgaattta tcattagtta tcatcccaag tacttcagta gaaatacgta
      541 tcatctcacg tttaatgtcg gattcttgca tagttggcac gctcgttcca acatctagat
      601 ctatgttata aatacccata atataaaggt cggctcgaat gttttgtagt tgcttataag
      661 cttgtacccc aatattgatc atagattgtt tataaaaagt accgccaata ttgataattt
      721 ccaaattagg atgctgtgct aatgctatgg caatcggcgg gctattggta atgattgttg
      781 cacgaaaatc ttgtgggatt tcttttgcta aagccaagtt agttgtactt ccatcaatga
      841 tgatgacaga ttcttctttt aaaaaaggga gggctttatg agctaatgca gttttttgtt
      901 cgctagctac agtttctcgg tagttaaaat tggtaattgg cggaccaaca cgtaaagcgc
      961 cgctgtgtac tctttttaag agaccttggc tatccaaatc tttcaaatct cgtcgaatcg
     1021 tgtcttcgga tacattgaaa cgttgactta aatcactagc gataactttt tgttctgcgt
     1081 ttaataaatc tagaattttt tgatgacgtt ctaatttcaa catgttgcgt gctccttctt
     1141 atttattctt gtttatcatg tcatttatgc gtttttatgt caataatttc cgtttttaat
     1201 acactaaaaa ggcataaaca agcaaaaaca aaatattttt atgcagtttt atgttgacta
     1261 gtatttgtta agcggttaca atgagggggt aattaaagga gggaatgtat ggagaaaaaa
     1321 attattttgt cgccttcact tatgtgtggg gatctagcaa atttggaagg aagcatccaa
     1381 gaactagaaa gtattgggat ggattccttg catattgacg tgattgacgg ttcttttagt
     1441 cccagtatgc ctttagggat cgaaacaatc aaaagaatta gagaaatcac ttcaatgaat
     1501 tttgatgtac acatcatggc taaggataac gataacgaat tttttattgg agaaatgttg
     1561 aaaattggcg tagagagcat tacttttcat tatgaaacaa gcctccatat tgatcgatat
     1621 atccagctca tcaagaattc tggaacaaaa gtagggatag ctttgaatcc agcaactccg
     1681 ttaaatgtat tacaatatat tttacccgag attgatagga tttgtttgat gctgattaat
     1741 ccaggttttg caacaaacaa gtcagaaaaa caagtttctt acgcgctaaa aaaaattaca
     1801 gatctttgcc agttagtaaa tgagaaagag ttagcagtag atatccaagt ggatggacgg
     1861 gtatcgttag aaacgatccc gggattatta gatgcaggcg caacaaatct ggtattagga
     1921 agtacaagtt tgtttcttaa aggaaataca ttagcagaaa atcaaaaact actttatcaa
     1981 gcgatgaagc aatcagttag gaaaaatcaa tagatgtagt ggtcctgaat aatagaaatg
     2041 gaggggaaat agttgagtta taaggcggcg aaacaaaata ttgtaagaga aattactgaa
     2101 acattagacc gtatttcttc aaaagatgtg gaacaattga taaagaaaat tgaaaaagcg
     2161 gaacgagttt ttttcgtggg ggttggcaga gtactgttag cgctagaagg aatggcaaaa
     2221 cgcttagggc atctaggaat tcagacagta gtcgtaggac aaatcactga accggcaatt
     2281 acagataaag atttattgat tgttggttct ggtagtgggg aaagtgcttt cccactaact
     2341 attgcaaaaa aagcaaagga atttgggtcc caagttgccc atataggatc aaacccagac
     2401 agcacaatga aagaatattc agatcttttt attcgtatac ccgtacaaac aaaattagcg
     2461 ttaccagagg aaatttcttc tatacagcca atgactagcc tgtttgaaca aagtgttctt
     2521 ttgttaggag atagtattgc tttagtgatg atagaagaaa aagcaattga tatgtcaaag
     2581 ttgtggcaat accatgctaa cttagagtag gtgaaaaatg aaagcggtac attttggagc
     2641 agggaacatc ggcagagggc ttattggaca aattctacat cagaatggat tcgatctatg
     2701 ttttattgat acgaatcctg aactgatcca tcaattaaat caagataagg gttatactat
     2761 cgaaattatt gatgaaatga acaggagtga tttcattgat catgttgtgg cactgaatag
     2821 tttatccgag ctagaagagg tgattacagc aacaaaaaat gcagatatct taacaacatc
     2881 tgttggcgca ataaatttag taaaaattgc accgatcatt gcgtaagcct taagtaagcg
     2941 atttgaacaa aagcatagca tcaatatcgt agctaatgaa aatgtgataa atgcaagtga
     3001 tgtgttgaaa aaagaaattt attctttgct gtcagaaaaa gaacaagaga attttgatga
     3061 atatgcatat tttgtgaata cggcaatcga tcgacaatct ttaggaaaag ttcttggagg
     3121 aaaaatgatc tctgttgtcg aaccttatta tgaatggata atggatcgac agcagttaga
     3181 tccaactaca tcttttgata tgaaaaatgt aaatttggtt gatgatatgg agccttacat
     3241 tgagcgaaag ctttatatcg ttaatgctgc tcatgcggca gtagcttatt taggcgcact
     3301 gaaaggatat aagacgattc aagaagcaat ccaaaatatg gagattttca atctagtcaa
     3361 gcaattttta atggagaata tagagtactt tgtacaaaaa tatcattttt ctagagaaga
     3421 attaacatct tttgtggaaa aaacattaaa ccgtcatggg aatcagaaac tctccgatga
     3481 aattactcgt gtaggaagtt cccctattcg aaaattaggc cctaatgatc gtttaattgc
     3541 tcccatagta aaactagatt catatcattt accttacgat gcaggaataa aagtaattgc
     3601 tgcgggatat cattatcgga atcttgctga tccagaagct gaacaactac gtcgaatgat
     3661 tgtaaatgat gggttgaaag caacgattaa aaagatttct cgtttggaag gtaagctgct
     3721 gaatgaagtt gtcgctgctt atgagtccca gatttagagg aggagaatat gctaaaagaa
     3781 caattgtcag gaaatacaat gtttatggat gcgatcgatt catggcaaga ggcaatccga
     3841 gtaagtgcgc gtcctttgat aggaaaggga ataatagagg aaagctatgt acaagcgatg
     3901 atacaaaatg tgttggataa cggaaattat attatattac tgccactagt agcaatgcct
     3961 catgcgcgtc cagaatttgg gagcaaaggg gtcgggatga gctttttatg tttagataag
     4021 ccagtcatgt tcccaagtga agaaccagta aaattcttct ttaccttttc ttctgattcg
     4081 ccaaagggac atttagactt aatggccaat cttggagaat tgctgagtga tgaaaaaatg
     4141 tatcaaaaat tattcgatgt gaagacagaa aaagcattgt tagatttgat aaagaagtaa
     4201 aggagagaag aaaatggagg tagcaaacaa aaaaaaacga gtctccatgc gagctaaagt
     4261 tcaagcgatt ggcggtttct taacaaatat ggtattgcca aatataggcg catttattgc
     4321 atggggaatt gttactgcat tattcattcc aactgggtgg ttcccaaatg aatctctgaa
     4381 tgaactcgta agtccgacag tcagctatct attgccgtta ctacttgcct atactggtgg
     4441 tcggatggtt ggtggtacac gtggagctgt tttaggtaca atcggaacaa taggtttgat
     4501 tattggtgct gacataccaa tgttcatggg agcaatgatt atggggccac ttggtgggtg
     4561 gatgatgaaa aaaacagatg attttttaga tgggaaagtt cctgctggtt ttgaaatggt
     4621 cgtcaataat ttttctatag gaattattgg tttcatactg atgtgccttt cttatctggt
     4681 agttggacca ataatccaat cagctaatca atttgtaacg agtgcaattg aagcattagt
     4741 ttcaactggt tttttaccga tactgtcctt aatcaacgaa ccggcaaaag tcttattttt
     4801 aaataatgtt attgaccaag gtgtttatta tccattaggt atgcaacaaa cattagaggc
     4861 aggcagatca atttatttca tggtagcatc aaatcctggc cctggcttag gactattgtt
     4921 agcttattct ttatttggta aaaaggcagc aaaacagaca gcaccaggtg caatcatcat
     4981 ccattttttc ggcggaatac atgagattta ttttccatat gtattaatga aaccactcac
     5041 tatattatct atgattgctg gtggaatgac aggaatcatc acttttgaat tatttgatgc
     5101 gggcctggta gcaggtccaa gtccaggttc tattttttct tatttagctc taacgcctcg
     5161 gggaaatttt ttgggtgtca tggcgggagt ggtcacagca acactggttt ccttttttgt
     5221 aacatcagta attttaaaag cagataaatc aacagatgaa gacagaaatt ttgaactatc
     5281 agttcaaaaa tcgaaaacga tgaaatcaga aggaagaaaa ttattaaaag aagaaaatgc
     5341 tagagaaata tgtaccgaga aaaaagagat gaataaaatt tcctttgctt gtgatgcagg
     5401 aatgggaagc agcgcaatgg gcgcaacaat attcaagaaa aaattgcaaa aagcaggtat
     5461 tactgatgtt gagataaaaa attatcgaat tgaagaggta ccagaagata gtgatttggt
     5521 gatcgtacat caagatttgg aggaacggac tagaaaagca catcctaaca tgaaaattgt
     5581 gacattaaag aattatttgc aagatccagt aatcaatgga ttagcagaag aaatcattgg
     5641 attaaaaaca gaagactaat ttatgggaat ttttgattaa cctttattaa aattattttg
     5701 cctaatataa aaaggttgtg acatggcttg tgtcgcaacc tttttttgtc gcacaaataa
     5761 ttaatcggat attttatgtt catatttttt gtgatcaacg agaactgatg gggcaaagat
     5821 acattttaga tacttttaac tgttaattta caaaacaagg tgaaacgaat agaaaaatga
     5881 aaaaatcgat acggaagaat atgtttgatt aaaacatgca tgaagagaag gaggttgatg
     5941 atacatgaaa aaaatatatt caaaggaaat cgattgtttt agaggcattg ctgttttagc
     6001 agtgatattt tatcatttgc caaatagttt gctgccaggc ggattgcaag gagtaacgat
     6061 cttttttgtg gtttctggtt ttttaatggc taaaaatacg ataagagatg aaaaaaacag
     6121 acaattcagt tttttacatt tttactataa gcgtatcaaa cgtatttatc catttttact
     6181 gttttctttg gtcgtattca ttttcatact agggattagc aacattcgat tgataggaaa
     6241 cattcgtagt gagctaccca gtttgatttt tggctataac aattggtggc agctgaatca
     6301 aggagtatct tattttgact cttactttaa tgcttcttta tttaaacatt attggtcgct
     6361 ttcagttgaa ctacaattct atttgatatg gccatttttg tttattgtca ttaagcgtat
     6421 gagaagaaaa caagtgtttt acttaatcta tacattgatt tttgtttcta ttctttttag
     6481 tttgttttta ccttcaacta aggcatatta tcatacgata gctaaacttt ttccattttt
     6541 attaggagtt tgggggtatt ttaatcgaat aactattggc cgtttctttg aacaaaacac
     6601 ttttagtaaa atctggcttt tattactagc tagtctttgt ctgattctgt tcccgatttt
     6661 tccttataca ctaaatgaac tactgatcag tatttgtttt gctctattgt tagccagtgt
     6721 cgatgatatg aacattgcaa aaaagaccaa aatcgctagt acaaaacttt cttttgtagg
     6781 aaaaatcagt tatgaacttt atttggtgca tttcccagta ttgattgctc tgacaaatct
     6841 attcaaacat caccattgga aatatttgga tatcgcaggt ctattcactg tgtttttatg
     6901 tacattactc atgattactt gctttagaaa aggagtgcaa aagatgagat cacccaaaat
     6961 ttggacagct atggcactaa tgataatcgg agtttctgta ctggtattac gtgctcctgc
     7021 cagtcgactg accgctgacc aaaatcagtt gcaaagttta ttaaaagaaa actctagtaa
     7081 agcaaatgca ggagaaaatc tgttgtcaca agagaagaaa atattattta tcggtgattc
     7141 tgttatgctg ggggcgtatc aggaactaaa ggagacattt aaaaaagaag cattggtaga
     7201 tgcaaaagaa tcaagacaaa tcactgcgtt accagaaatc ttaaaggagc ataaagattt
     7261 agtttcttac caacaaatag tgatcgggtt aggaaccaat ggtgtgataa cagatgaagc
     7321 tatagaacaa acaatgtctt tgctgaaaga taaaaaagta tactgggtaa atataaaagc
     7381 accaactggc tggcaagata cgataaatgc cacattagct acattgccaa atcagtattc
     7441 taatataacg atgatcgatt ggtatagtga gagcaaaaat catcctgaat ttttttatga
     7501 tgatgaaacc catttgaatg aaagtggacg tacagcttat gcaaagtata ttgcttcagt
     7561 tattcaataa ggtagtgatt acgtgtgtta accttacttt ttcctaatag gtgaatcaat
     7621 ttaaataaaa aatacttcat atgataaacg gtgtttaaga ggaggagctt tttaatattc
     7681 gtggtaaaaa agagatgggc gaggtcgtta actgagagct tcttggaaag aaatcgcttt
     7741 ggatttatcc tctttgttat acagaacatg agcgagacga caatactagg atgtatgttc
     7801 tgatcgaagt tttatggtta caagcagcaa tcagcttaga taaaattaag gatcgtagga
     7861 accaagtaaa gaaggctgtt agtaaagaaa caaacgatca agtatgtttt actcttaaga
     7921 agatgcagtg aataaataat agtaatataa tgctagaagg gactgtgaca aaagacgtgt
     7981 cacagccttt tttgttattt taattatcgt ccagtctttt tttataaatg attgcttaag
     8041 cattaaatat taaaaaatat agagttcaaa attactgcac aattagatga aaaatttgaa
     8101 caacaaaaag tgtattttta tactataata gcttttgaat ttagttactc tattcaaaag
     8161 ttatgctatg gacaagaaaa agaataaata gaggtgagaa aatgaatagt ttcaagaaaa
     8221 aatttgtgag tagattttta aatatgaaaa atttttttga aaaaaaggga gattatgatg
     8281 catataatta tgtattaaat ctagaaaagc aaacatatta tacactacgt aaaaacgatg
     8341 ataatgactt ttggactatg ttacctaaaa tgctttcgat tgactctaag ctttcccttt
     8401 tagaggaatt tattaattta gacattgaag aattttgcgg gggaaaagag ttaattaata
     8461 tggtagagag tgattatcat acctattata aagaaagttg tggatacaaa gtggatgagc
     8521 aatgtccaca atcaattatt ttttacattg agtaatatga actattaagc aggaaagcaa
     8581 ctagaagcta ctgatttagt atttacaaat ataaactata ttgctaacaa ttattttcta
     8641 gaaggaataa aagaactaag aatcaagacg ttatcttgat tcttagttct tttaacatgg
     8701 aaaagatgga taatgcactg ttaattcatg atgcatcatc agaaaagttg gaaggagaag
     8761 atagaaatca ttaaatgatt tctacagatg gtaaatgaaa aaacaaaata ttagcaaaaa
     8821 tttgcctaaa tagatagtaa cactttctaa attaaataca agcgcattca taaaatgttt
     8881 atacaaatat ctatgtgata aaaattggat atgttcacaa taaataggaa ttaaaataac
     8941 gaatttacgt ttgtttttat ttaccaatca aattatctta atgcaggaat aaaaaattaa
     9001 attacatatt gttaatcatt tacatctcca ttaataactc aaaatgaaaa gatgttttta
     9061 tttt
//
LOCUS       DAZUMO010000097         8964 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000097 DAZUMO010000000
VERSION     DAZUMO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.097"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..276)
                     /locus_tag="ACG8ER_002540"
     CDS             complement(<1..276)
                     /locus_tag="ACG8ER_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051069.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            611..2002
                     /locus_tag="ACG8ER_002541"
     CDS             611..2002
                     /locus_tag="ACG8ER_002541"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5051070.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            2061..2864
                     /locus_tag="ACG8ER_002542"
     CDS             2061..2864
                     /locus_tag="ACG8ER_002542"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5051071.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2897..3751
                     /locus_tag="ACG8ER_002543"
     CDS             2897..3751
                     /locus_tag="ACG8ER_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFS5051072.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3748..4449
                     /locus_tag="ACG8ER_002544"
     CDS             3748..4449
                     /locus_tag="ACG8ER_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFS5051073.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4457..5524
                     /locus_tag="ACG8ER_002545"
     CDS             4457..5524
                     /locus_tag="ACG8ER_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5051074.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5517..6482
                     /locus_tag="ACG8ER_002546"
     CDS             5517..6482
                     /locus_tag="ACG8ER_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5051075.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6527..7501
                     /locus_tag="ACG8ER_002547"
     CDS             6527..7501
                     /locus_tag="ACG8ER_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5051076.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7524..>8964
                     /locus_tag="ACG8ER_002548"
     CDS             7524..>8964
                     /locus_tag="ACG8ER_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS5051077.1"
                     /translation="MKKSRLEYSLINSSISMFIYILRIVIQFVGRSFFIYFLGAKYLG
                     LNGLFTNILSFLSLAELGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIG
                     IVGVLIIPLLPLFIKNSTDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAI
                     NDFAFLFFMNIVQVIALYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVV
                     SLDKQTKDEVKKNVIGNMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQ
                     NLCKQITNSITASVGNFAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISL
                     WVGAKYMLPMSTTILIVLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSL
                     LLLIVFDMGINGVLIGTIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELII
                     SAIVVFLLNEYLANGGSTFLQFITKTLITI"
BASE COUNT         3168 a         1220 c         1646 g         2930 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgttatatga atattgtaac ttgtgaataa ggaattagca ggagaatact
      421 agttcagcat aaaggaagtg atgggacttc tgagacaact ttctgttatg cagaaataca
      481 catataaaca gacaataaac gcttttcccc gtttctttta atttccttta caatttggtt
      541 attcactagg tttttaaact ttgctatggt aagatgatag aggcattatt ttatggttag
      601 gagtgtatat atgaataaaa atggggagtg gaatgcggca cgcagaatat tcattattct
      661 tatggatgta cttgtattaa atgtatcgat ctatcttagt tttttgctga aattccaggg
      721 agaagtacca gtgagaaact tagaaacctt tcagcattca gcaattttta tttccatcat
      781 ctttattgcg ttgaatattt tgttaggagc ttatgtattc tataaccgaa tgatcagtga
      841 tattgtcttc attactgtta ttggacaagt actaatagca ttggggatca tggtagttac
      901 ttttgctggc cgatggtttg catttccgcg tggcgtcatt ttgatctcgt ttatcctagg
      961 tacgatatta cttagtgttt accggatagt cgtttacaaa ctgtacttaa gagtcagtcg
     1021 tgataagaaa gtccttatcg tgggtttgga gaaagatgtc gcaccagcta tccaaaattt
     1081 tcaatctaag aaaaacaata aacataaggt tgaagcagtg gcaatcgata attattacga
     1141 aaatacaaaa aaattgatcg atgatatgga tatcgtctat ttggcttcta aaatcccaga
     1201 agatgaaaaa atcaacattt atcaattagt gactaaaaaa gagaagaaat tatttttaaa
     1261 tacgactttt gagaatctga caatgataaa tccgaatatt atgaattttg aagatgaaag
     1321 catcattgaa gcttctgggt tcagaatcca accagaatac gaactattca agcgtctctt
     1381 tgatatcatc gtttcgctta ttttactgat cctcgcctca ccttttatgc taatcacagc
     1441 gattttggta aaaacaactt cacctggacc aatcatctat aaacaagtac gtattacgaa
     1501 aaatcaaaag gaattttcaa tctataaatt ccgttccatg agtgcgacag ctgaagcaaa
     1561 atcaggacca gtcttggcta aaagtaacga tgcacgtgtg acaccagtag gtaaatttat
     1621 ccgtgctgta cgttttgacg aattacctca aatctttaat gtattgaaag gcgatatgtc
     1681 gattgtcggt ccaaggccag aaagaccatt tttcgttgat cagttcaatg aagaaaatcc
     1741 ttattattac ttaagacaca atgtacgtgc gggaattacc ggatatgcac aagtatacgg
     1801 aaaatatgta tcagattata acagtaaact acgttttgat ttgttgtata tcaaaaaata
     1861 ttcattacta ttagatttga agattttact tcaaacaatc aaaatcttat ttgataagat
     1921 gtcatctcaa ggactagatg aagatgaaaa tattagttca ttagcagaaa tcgacttttc
     1981 acaagagaaa atttatcgct agattggaaa aattttgagc aaagaggtag gagagaacat
     2041 tggaaaataa aaatgtaagt atgctgcttt ctttatatga taaagaaaag ccagagtatt
     2101 taaaggaagc attaactagt atttttgagc aaacagtaat gccaaaagaa attgtattag
     2161 tttatgacgg ccctataaat gaaaaacttc aagtagttgt cacttatttc caacaaaaag
     2221 atcctcactt gtttacagtt gtaaaattag aaaaaaatca aggattgggg attgctttag
     2281 caattggatt agaacatacg aaaaatcgct tagtagccag aatggataca gatgatataa
     2341 tggaacccca acgattagaa aaacaattag agatcttcca aaaagatcca gaaatagcga
     2401 ttgttgggag taatattgag gaatttactg gcacgcttag caatgttatc ggaaaaagaa
     2461 ttgtacctga atataacaat gcaattcgtg tattttctaa aagaagaaat ccctttaatc
     2521 atatgaccgt tatgtttgat aaaaaagcta ttttggaagt gggaaattac caaccattaa
     2581 gtgggtttga agattattat ctttgggcaa gattattaaa ggcaggttat aaaggatata
     2641 atattcaaga aacattagtc tatgcgcgaa ctggcaaaga tatgtatgca agaagaggcg
     2701 gaatgaaata tcttggttct ggtcttatgg gtcgttatcg tatctggaaa gacggtttag
     2761 ggtcagtaaa agattttata attgtttctt gcggacatat ttttattagc ttactaccaa
     2821 atcaattaag aggaaaattt tatcaaacgc aattaagaaa ataggaggtt tttctttgta
     2881 tagcactgat ccaaaaatga aaaaaataca acagattacg ttgaaaatgt ctcaagagat
     2941 tgttgatttt tgtctaaaaa atcaacttct ttgttatttc tgcggtggtg gtgccatcgg
     3001 tactgtgaga gaacaagggt ttattccatg ggatgatgat ttagatttct ttatgcctag
     3061 agaggattat gaaaaatttt atcaactatg ggttaattca gaaaaaagta aaaaatatcc
     3121 catacagaag gcttctaaaa attataatga tcataattct tttacaacta tccgcgatgc
     3181 agatactaca tttattaaaa cttatcaaag agatttagat atagtccatg gaattactat
     3241 tgatattttc ccgttagatg ttgcccctgt ttcgaaattt agccgtaaaa ttcaaaaact
     3301 atgggcttta gtatatgctt tattttgttc tcaagtcgtc cctgaaaaac atggtggaat
     3361 aatggctaca ggtagtaaga tactcttatc tattttccgc tcacctcgta cgagatataa
     3421 gatatggtct tttgctgaaa aacaaatgac aaaatataat aatgaaccaa cgggctatgt
     3481 gactgaactt tgtgtcggac ctaaatacat gggaaatgtt tatcctgcaa aagactttga
     3541 atctgctgtg tgggtgccat tcgaagatac agagatgcca gtaccagttg gttatgataa
     3601 ttatttaagt caagtttttg gtgactatat gcagcttcca cctgaaaaag atcaggtttc
     3661 tcatcatgaa gcagtttata ttgatccaga acatccatac acaatgtaca aaggaaaata
     3721 ttatttaact aaaggagcag aaaaaaaatg attacagcta ttattattgc tggtggtgtg
     3781 ggtaaacgaa tgggacaaga tatcccaaaa cagttcatta caattgaagg aaaacctatt
     3841 attatctata ctttagaatc atttcaacac catcctcaag ttgaccggat tttagtcgtt
     3901 tgcaaatctg gttgggaaga aacgatgtgg gcgtatatca aagaattcaa tattagcaaa
     3961 gtcaaatggg tcattacagg tggatcaaag ggacaagagt cgatcaataa tggtgttcag
     4021 tttttgaaag actattctga agatgatgat acgatcatca tccatgatgg aatccgtcca
     4081 ctagtagacg aactggtatt gtcagatgta atcgttaaat gtaaagaatt tggtaacggt
     4141 gtaacctctc taccttacaa cgaacaaatt tttatcaaga aaacagaaga aacgacggaa
     4201 cagtatgtcg atcgtaacac attgaggcgt gtctcaactc cacaagctta tcaatacgga
     4261 aaactacggg cagcatatga ccgggcagtg aaagaagaca tcggtatgac tgattcttct
     4321 tatacgaata ccatgatggt agatttggga gaaactttat attttgctgc tggttctgat
     4381 aaaaatatca aattgacaac aacagatgat ctagaattgt tcaaagcata tttaaaaatg
     4441 aaagactagg tgaaagatga tgacgttgat taaacaacca atgtaccaag aagatttggc
     4501 aaaagtaatc aatcatacac caaaaataaa acagctacat ggaaaatccg ttctgatgat
     4561 tggtgcttct ggaatgatcg gctctttttt gattgatacg ttgatggctg ctaatgaaca
     4621 actaggcatc aaaatcaaag tttatgcaat gggcagaaac cgtcagaagc ttgaaaaaag
     4681 gtttgccagt tatcttgaat tagatacttt cgaaattgtt gaaggtgacg tcacagaacc
     4741 cttaccaaaa gaaattcaag ctgactattt gatacacggt gcaagtaaca cacatcctaa
     4801 ggcttacgca actgatccaa tcggtacgat catgactaat cttgcaggta cagagcaagt
     4861 attgaaacat gcagtagcaa cccaaccggt tagagtatta tttttatcaa cagtagaaat
     4921 ctatggcgaa aatcgaggcg atatagaaaa attcacagaa gattactgtg gatacatcga
     4981 ttgtaataca ctgagagcag gctaccctga aggaaaaaga gtcagtgaat cattatgcca
     5041 agcctacatc gcaaaacacg acctcgacat cgtgattcca aggctttgcc gtacgtttgg
     5101 tccgacgatg cttttatctg atacgaaagc atcctcacaa tttattttaa atgcagtcga
     5161 acaaaaagat attgttttaa aaagtgaagg aaaccaatat ttttcgtatg tatatgttgg
     5221 agatgctgtt tctgctattc ttcatttact gcttgaagga gaaaaaggag aggcgtacaa
     5281 cgtttcttca gaaaactttg atttacgact aaaagaatta gcccaacagc tagctgatat
     5341 cagcggcaaa caagtaattt ttgaattgcc ggatgagatt gaacaaaaag gtttctcaaa
     5401 agctagcaca gcagtacttg aaaataaaaa aattaaagag acaggttgga aaccaatttt
     5461 tcctttgaac gaatcattgg ttcatacagt agaattagtt aaaggtgaga aatataatgt
     5521 gtgagattag tattattgtt cctgtgtata aagttgaaaa atatttaaga aaatgtgtag
     5581 attcgatttt agcccaaaca tttactgatt ttgaagttat attagtagat gatggatctc
     5641 ctgacaatag cgggaaaatc tgtgatgagt atgctgaaaa agataatcga gtacgtgtga
     5701 tccataaaga aaacggcgga ttaagcagtg ctagaaatgc ggggattgat gttgcgagag
     5761 gcaaatactt aggctttgtt gacagtgatg attatattga tgaagatatg tatgaaattt
     5821 tgtatgagaa tttgaaaatt catgatgccg acattagctc tgttgaattg attccttttt
     5881 atggtgatag atataagaaa gctaataaag aaaagaaagt aattatatta aataaaaagg
     5941 aagcaataaa atctgtttta gaaggaacgc aattttatgc atacgcatgg aataaacttt
     6001 atcgtaagga actttttaaa gataatcgtt acttggatgg aaaaacattt gaggatgcat
     6061 atataataat agatctatta tttcaaacag aaaaaattgt tgtttccaat gaagagaaat
     6121 atttctatct tcaaagaaat gatagtatta tgggaaaaag tttttctatg aataatttcg
     6181 atgttataga agcatggcaa tataacaaag agaagatttt agatgctttt ccagatttgc
     6241 atgattccta ttatcgccga ctatgttggg catattttta cgtgttggat aaaatggttt
     6301 tatcaagtag ttatcaacaa attccacgta aaaaaacagt aattgaattt ttaaagaaga
     6361 acagaaattt tatattaact tataagggat tcactaaatc gcgcaaaata gctattattg
     6421 ctctgagctt tagagtagat tggtatagga aattggttga acaaaaaaat aaaaaaaatt
     6481 agaatgttta aattagtaaa taaaagaaaa ggaattgaaa actaaaatgt gcagaataag
     6541 tataatagtt ccagtttata atgttgaaaa atatttaaaa aagtgtgtcg attcaattct
     6601 aggccaaaca tttaccgatt ttgaattatt attaattgat gatggttcta cagataattc
     6661 aggaagtata tgcgatgaac ttgcgaaaac agacaaccga ataaaagtga tacataagga
     6721 aaatgggggt ctaagtgatg ccagaaatat tggtattgag gtagcaaagg gagattttat
     6781 tgggtttata gatagtgatg attacattga tgaagatatg tatgcatttt tatataacaa
     6841 tatattgaaa tatgatgcag agttatcaat gtgtggtatt tatgacgtcc ataagaataa
     6901 agaaattaaa aagttaactc ctttttctca attggttact aaatctgaag caattgaatt
     6961 agtattagat ggtaagctag ttgttgctaa tgcagtaagc aagctatata aaaaagaatt
     7021 atttgagaat gtgagatatc caaaaggaaa aattgctgaa gacgcagcag taatactgaa
     7081 aattatcaat cagtgtaata aaatccatgt tgatacttct caaaaatatt attattatca
     7141 tcgtggagat agtattacta gtaaaaaatt tacaaaaaaa gattttgata taattgatgt
     7201 atggaatgag aatgaaaaat ggattattaa aaattatcct caaatgtacg ataaagtaca
     7261 tactagagtt tgttgggctt actttgttgt gttagataaa ttagttttaa ctgaagaaaa
     7321 aggactgtat caagaaaaaa aacaagtaag agagtattta attgataatt tcttttttat
     7381 tataaaaaat aaacgattaa cttttcaaag aaaaatatct atgttattgc ttacttttgg
     7441 aattgctgcg tataaaatac cggttaaata tcagtataag aaagtaaagg ctgttaatta
     7501 atgctttagt taggatgaat tagatgaaaa aaagcaggtt agagtattct ttaataaatt
     7561 cttctatatc aatgtttata tatattttaa ggatagtaat acaatttgta ggtagaagtt
     7621 tttttattta tttcttagga gcgaaatatt taggattaaa tggtttattt acaaatatac
     7681 ttagtttctt gtcgttggct gaactaggca taggctcttc tattatttat tccctataca
     7741 aaccattagc ccaaaaggat gaaagtaaag taaaggcttt aatgaatttg tataagaaga
     7801 catatgaatt tattggtatt tttatcggca tagtaggagt attgattatt ccgctgttac
     7861 ctctatttat taaaaatagc acagacatac cacatatgta tcgatattat gtattatttt
     7921 tattaaattc agttcttagt tattttttca cgtataaaag atcacttgca atagcagatc
     7981 aaaaaaatta tctggttgcg attaatgatt ttgccttttt attctttatg aacattgtac
     8041 aagtaattgc tttgtacttt tattctaatt ttactctatt tctaatagtg cagattttgt
     8101 ttactctatt ttctaatata aatatttcca gaacagttga caagagatat tcatatttaa
     8161 aagagaaaga tgtagtttct ctagataagc aaacaaaaga tgaagtaaag aaaaatgtca
     8221 taggtaatat gtcctcaaaa ataggcgggg ttattgtaat ggggacagat aatatattaa
     8281 tttctacatt tgttaattta acagctgtag gaatttattc taactattca ttgattgtca
     8341 caagtgtgca aaatttatgt aagcaaatta ctaattctat cacggcaagt gtaggaaatt
     8401 ttgctgtatc aaataaaaaa aaggattcat ttgagctatt taaaaaacat ttttttatca
     8461 accactcact aatctttttt tcttctatat tgttattgac tttaatgaat cccttcatta
     8521 gcttgtgggt aggagccaaa tacatgctac ctatgtcaac gactatttta attgttttaa
     8581 attatagtgt acaggtttat agaaatacat ccttcgtttt tatagagtca tttggtttat
     8641 attggtctca acggaaaaag cctataattg aagcaggtgt aaacttgtta ttatctttgc
     8701 ttttgttaat agtttttgat atgggaatca atggagtact tatagggact atcggatcgt
     8761 ctctaggatt tgtgatttgg tatgaagcat ttatcatata taaaacggta tttgagaaac
     8821 gttttattca tttcttttta actttctggc gaagttttat agaattaata atttcggcaa
     8881 tagtcgtttt tctattaaat gaatatttag caaatggagg cagtacgttc cttcaattta
     8941 taactaaaac cttaataact atag
//
LOCUS       DAZUMO010000098         8589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000098 DAZUMO010000000
VERSION     DAZUMO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.098"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..1514)
                     /locus_tag="ACG8ER_002549"
     CDS             complement(48..1514)
                     /locus_tag="ACG8ER_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS5051078.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="ACG8ER_002550"
     CDS             1901..2497
                     /locus_tag="ACG8ER_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5051079.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="ACG8ER_002551"
     CDS             complement(2555..3037)
                     /locus_tag="ACG8ER_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051080.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="ACG8ER_002552"
     CDS             complement(3143..3340)
                     /locus_tag="ACG8ER_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051081.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="ACG8ER_002553"
     CDS             3635..4393
                     /locus_tag="ACG8ER_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5051082.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="ACG8ER_002554"
     CDS             4453..4881
                     /locus_tag="ACG8ER_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051083.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="ACG8ER_002555"
     tRNA            4927..4999
                     /locus_tag="ACG8ER_002555"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="ACG8ER_002556"
     CDS             complement(5149..5520)
                     /locus_tag="ACG8ER_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5051084.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="ACG8ER_002557"
     CDS             5735..5920
                     /locus_tag="ACG8ER_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051085.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="ACG8ER_002558"
     CDS             complement(6003..6191)
                     /locus_tag="ACG8ER_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051086.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="ACG8ER_002559"
     CDS             complement(6215..6478)
                     /locus_tag="ACG8ER_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051087.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="ACG8ER_002560"
     CDS             6636..6893
                     /locus_tag="ACG8ER_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS5051088.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="ACG8ER_002561"
     CDS             complement(6964..7155)
                     /locus_tag="ACG8ER_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051089.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="ACG8ER_002562"
     CDS             complement(7296..7616)
                     /locus_tag="ACG8ER_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051090.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="ACG8ER_002563"
     CDS             complement(7639..8073)
                     /locus_tag="ACG8ER_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5051091.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8408..>8589
                     /locus_tag="ACG8ER_002564"
     CDS             8408..>8589
                     /locus_tag="ACG8ER_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5051092.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2720 a         1695 c         1362 g         2812 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acgtggattt gtcataaatt gtcatgcttc ttttacctat gaaatatttt
     8341 cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg aataaaggag
     8401 atttttcatg acatcactta aaactttact ttttggaaca actttggctg ccggcgctgc
     8461 attcttcatg ggaacgactg ctcatgcgga cgaagcttat accgttcaat caggtgatac
     8521 tctatcaacg atttctcaaa aatacgttgg tgataactcc ttgatcaatg ccattgcaga
     8581 atcaaattc
//
LOCUS       DAZUMO010000099         8501 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000099 DAZUMO010000000
VERSION     DAZUMO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.100"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..655
                     /locus_tag="ACG8ER_002565"
     CDS             <1..655
                     /locus_tag="ACG8ER_002565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5051093.1"
                     /translation="LKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIANQN
                     HDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFE
                     CPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCR
                     EILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            670..1422
                     /locus_tag="ACG8ER_002566"
     CDS             670..1422
                     /locus_tag="ACG8ER_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5051094.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2125..2340)
                     /locus_tag="ACG8ER_002567"
     CDS             complement(2125..2340)
                     /locus_tag="ACG8ER_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS5051095.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2359..2595)
                     /locus_tag="ACG8ER_002568"
     CDS             complement(2359..2595)
                     /locus_tag="ACG8ER_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS5051096.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2853..5006
                     /locus_tag="ACG8ER_002569"
     CDS             2853..5006
                     /locus_tag="ACG8ER_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS5051097.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4975..6390
                     /locus_tag="ACG8ER_002570"
     CDS             4975..6390
                     /locus_tag="ACG8ER_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS5051098.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6423..7457
                     /gene="sppA"
                     /locus_tag="ACG8ER_002571"
     CDS             6423..7457
                     /gene="sppA"
                     /locus_tag="ACG8ER_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5051099.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7459..7980
                     /locus_tag="ACG8ER_002572"
     CDS             7459..7980
                     /locus_tag="ACG8ER_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5051100.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7977..8363
                     /locus_tag="ACG8ER_002573"
     CDS             7977..8363
                     /locus_tag="ACG8ER_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5051101.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         3053 a         1214 c         1517 g         2717 t
ORIGIN      
        1 attgaaaaga aaagaattgg tttatctaaa aaaatatact gattcactgg aaaaagatca
       61 agaaaaattt gccagattta gacatgacta taaaaattta ttgctgagtt taaaagaaat
      121 cgctaatcaa aaccatgatt cgatattgga aaaagaaata aaagaattag agatctattc
      181 taattcttat ctaaccaata agtttgaata tcgttattta aaaaatataa aaaatgaata
      241 tttgaaaagt ttgttgatag cgaagctata ccaagcaaat aacaataaga tttgttgtaa
      301 attcgaatgt ccaagtacta ttattgaagt tcctgttcca atatttgact gtattagggt
      361 gcttggaatt attctcgata atgcaattga agcatctata gagagtcaag atagagaact
      421 gtctctagct atttaccgag atgataaaca gatagaaatt tcgatatcga atagttgtag
      481 agagatacta ctctctatag atactctaat gaaaaaagga ataacaacaa aaaagggaca
      541 tcaagggtta ggtttatcta gtatagagga aatcaataaa aagaatccca atatgtttgt
      601 gaactatcaa aacgatccgt atcaatttac tactcaagtt attttgttgt tttgagggaa
      661 ggaggattta tgagttatcc aataatatta tgtgaagatc aaattataca attaaatcag
      721 ttagaacgaa ttattgataa tttcattctt tttcacgata aagtatttaa gatagtattg
      781 aaaacacaaa gtccgttaga agttaaaaaa tacctcaaac aattccgacc aaagcaagga
      841 atatatttct tagatattga tttaaatcat gaagttaacg gtatagaatt agcagaagta
      901 atcagaaaat atgatgttca agcaaaaatc atttttacaa ctactcatga tgagatgtta
      961 cccgtaacaa taaaaagaag agttgaaacg ttaggatttg taacaaaaga tcaaacacta
     1021 gatgagtatc gaaacgagat tgttgagtta ttgttattag cgcaagaaag gatagatgca
     1081 acaaaacagg ttacgaatca agcatttgtt ttttccatag gttctcaaac atttactttt
     1141 gatataaatg acatttattt tgtagaagct tcaaaattac cacatagatt atcattgtgt
     1201 actaaagatg ggcagtacga attttatgga agaattagtg agcttgaaaa gaaatatcct
     1261 atgttaacaa gaattagtcg agcgtgttta gtaaatatct ttaacgttaa agaaatagat
     1321 tttaaaaaac gttcactata ttttgattca gagttggcca gaaactttac tttagggaaa
     1381 gcacaaaaaa ttaaggagaa gttgaaagaa agcactgttt agtggttttt tctttaggga
     1441 gattgcattt ttctaataaa atagattcct tcactagtgc aaatttttct gaatcctatt
     1501 aaagttgtac caatttttat agctgttatt agttacgtgc ataacagcaa tattagttac
     1561 tggaggaata tttctatggt ctggaaccaa aaaagggcca aatgaatagt tcttttcttg
     1621 tttttattaa gtaccataaa ggggaattaa aattaaatgt atggtggaag atgcgaaaga
     1681 tttggcaaat tctactaatt tttagaaagt gtttttcatt ttttagttaa aaaatttgtc
     1741 ctgtcaagtg caatcctatg attgtacttg ataggatttg agataattac tttcctgttt
     1801 ttataaattt aacttgcagg aacaggcttt aatgcacatc cgacaagtac attaccaata
     1861 cctcctgtaa cggccgaaat aggtcctcct gcagcaccaa ctacagcgct gcaagtcctt
     1921 ttgtaaaaat atctctacca ttacatttcc aaagaatttt tatctgactt taataaaaga
     1981 taaaaattta atataattat aaaaactagc acaagaagct ttagtaaaaa gttctgtcca
     2041 ttccaaactt ttgaaaagat aaagaacata cctatgttcg taatcgacac aaaccaatct
     2101 tgaggtttga tattttttaa ttgtttacgc accggtccac atgctgaatc cagcacctaa
     2161 taaagctcca cctacaccac aagcagctat tgcataaggt cctccggctg aacaaagttt
     2221 tactcctgct gttgccccag aaaaagtagt gcctaaaact cccacggcac tcccaccttg
     2281 aatatttttc aactcttctg aagataatat aaatgttgtt tttgtgtttt taatatgcat
     2341 actacttatt ctcctttttt atttttttcg agcatcccag ccagctgcag cacctccagc
     2401 gacagctcca tataaccctc caacaacacc gcaagctggt ccgcaaattg ctgttcctaa
     2461 acctgcacca cctactgccc ctgtggtaag tccacctaca acttgcccca attttcccca
     2521 agcattattg cctccaccaa cagtttgtgc atttccacaa tacttagttc ctttacattt
     2581 tgcatgttta aacacgcttc tttaaataat attttttacg gtgctactgt aacatacgta
     2641 tttgattaac agtaaaaatc ttcctgaaac ttatcaagtt tattcttgaa tagtaaaatt
     2701 tccaaaataa atttgattca ggaatgaaaa agttagtgtt caagaactgt tttcaaagtt
     2761 tttgtggaat tatgagacaa tattttcact gtattaaagc tatattgaga atttttctat
     2821 aaaaattttt tagaaggagt tgtgttagat aagtgataaa atcatttcct tttgtgcagc
     2881 aacaagatga aaaagattgt ggtgttgctt gcctttcgat gattttaaaa tattttaaaa
     2941 ctgatatccc gattcataaa ttacgggacc tatctgggac tgatttagag ggtacttcag
     3001 catttggatt gaaaaagaca tttgaaaaat tgtcttttga ttgtatggct attcaagcag
     3061 atagtgagat ctggaaagaa agagatctta tcttaccatt aattgcacat atattggttg
     3121 ataacagcta tatgcactac gttgtaattt ataaaacgaa gggaaacgta ctatggattg
     3181 cagatcccgc taatggaaaa aatagaaaaa cgattgaagc gtttcaaaaa gaatggacag
     3241 gtattctttt attacctatg cctagagaaa cttatattcc tattaaagaa aaagtagctg
     3301 gattaagttc ttttttgcct ataatttgga aacaaaaagg attagtattc cacatcgttt
     3361 tagcatcgat tttcattacg ttttttggca taggaagctc atattatttt caaggtattc
     3421 ttgatttttt catccctaat caagcacgtt caactttaaa tataatttcc attggcttga
     3481 tcgctgtcta tttatttcgg gtattgtttg aatatagtcg tagttatctt cttattattt
     3541 taggacagcg aatgagtatg gcaattatgc ttcagtattt caaacatgtg ttaactttac
     3601 caatgaattt ttttgctacg agaaaatcag gggaaattat ttctcgtttt cttgatgcca
     3661 gtaagattat tgatgcttta gcaagtgcta cgttatcaat atttttagat attggtatgg
     3721 tgttgcttgt tggggttacg ttagctattc agaatagtac gttgttttta atcactctat
     3781 gctctttgcc tttttatgta gttgcaatat tcgctttcgt gaaaagctat gaaaaatcga
     3841 acagggaaga aatggcagcc ggtgcagctg taaattctag tatcatagaa agtcttaaag
     3901 gaatagaaac aattaaagca tatagcggtg aagagaaagt atataaccgt atagaccatg
     3961 aatttgttaa attaatgaaa aaatcatttc gtacggttac gcttgataat attcaacaag
     4021 gaattaaaca tttgattcaa ttaattagca gtgcattaat tttatggcta ggttcttatt
     4081 ttgttatgga taatagaatt agtctaggtc aacttatcac atacaatgct ttactagtat
     4141 ttttgacaga tcctttacaa aacattatta atttacaagt gaaaatgcaa acggctcaag
     4201 ttgcaaataa tcgtttaaat gaaattttcg cgattgattc agaaaaatcg aaccagtctt
     4261 ttgaatatat gctttctgaa caattatttc aacaagatat cactgtaaaa aacgtttcat
     4321 tctcgtatag catgaaggcc ccaacgttga acgaaatttc atgtacaatt ccttcgcaca
     4381 gtaaaacggc tatagttgga gttagtggtt ctggaaaatc aactcttgca aagttatttg
     4441 ttcgttttta tgaagcatca gaaggatcga tccattatgg aaacattaat cacttagaca
     4501 ttccttttca gatattacgc gatcgagtga catatttgcc tcaagagtca tttttcttta
     4561 gtggaacaat cattgagaat cttatattcg gtttagatag cccaccaagt ttcgaacaaa
     4621 taatagaagt ttgtgagata gtccaactaa atgaatttat taatcaacag ccattacgtt
     4681 acgagacatt gttggaagaa ggtggaaata atctttcagg agggcaacga caacgattag
     4741 ccttagcacg ggctttatta aaaaatccgg acatattaat tttagatgaa gcaactagtg
     4801 gattagatac cttgttagaa catgcaatta tgaattatct cctcaattta aaagaaaaaa
     4861 cgattatttt tattgcgcat catttaccaa tagcaaaagc atgtgatcaa attttagttc
     4921 tccatgaagg gcatttggta gaaaaaggaa cgcacgatga acttcgtttt gccgatggta
     4981 tctatcaaaa attatgggaa atctaggaga gggaaacaat ttatgcagaa taataattgg
     5041 ttcgaacatt ctagtatcta tactaaacaa catcatagct tctaccgttg ggtgttatac
     5101 cctgtaattc tttttttatg tattattgga ttgtttttaa cctttgctaa aaaagaggtt
     5161 gttattcgga caacggctca attgaacccc gaaaaaatag agaagttaca agtgccatta
     5221 gaagctaaaa taatggaaaa cttcctaaaa gaaaatcaat ttgttcataa ggacgatgtg
     5281 ttggttagat tggattgttc tttaatagag aatgaaaagg ctcagataga acaagaaaac
     5341 caacgtatta ctcaacaaat aaagatggct cagctattta ttgaaagtat aagtaaagga
     5401 aaaaatttgt tttcaacgga tgacagtttt ggctacagta atcaattaaa gagcatgttg
     5461 tcagaaaaag aatcactccg ctacgctttg aagcaaagtg aattaaatga tcaaaagcaa
     5521 ttagaagtat acgaaaagac aaaaagacaa ctagaaaaac aaattgagag ttcagatagt
     5581 aaattacaag aatggcaaca agtacaggta gcttggagta ataatcaatc attaaaagat
     5641 ttttcaaaag aaatgatggc aaactatgag aattggcaag aacaactaaa taatgtttct
     5701 gatgatcaaa aaaatcaagt gaaactgaca atttcagcaa gcataaatga acaaattgag
     5761 caactaaaaa aagaagtaga acagtatcag tcagaaaaag ctaaattagt taaaccaact
     5821 acttctgaga atgacagaat tagtcaaacg gaaaaaggaa agcaagagct agaacaagca
     5881 ataacaactg taaaacaaac agttacagag ctaaatgaaa aacaagagaa gaaccagtca
     5941 gttatcaaaa gtttggacga tcaactttca aaaggaatac taaaagctcc tgtatctgga
     6001 acggttcatt taaatgaaga aacaaaggga caagcagaac tagccaaagg aacagtgctt
     6061 gcagaaattt atccgatcca cgaaaaatca aaaatgaaat ttacagcact tttacctgcg
     6121 aatgaaagta cctatattaa agaagggatg aaggttcatt ttaaattgga tcaaaaaggt
     6181 ggggctccga ttacgatcga cggaacttta gatgaaattt cagaaaattc tactgccact
     6241 gagaaaggtg ttttttatgc agtgaaagga acgttaaaaa caactcaatc gtttccgttt
     6301 cgttatggat taactggaga gttgtcttta attgtaggag aaaaaacata ttggcaacaa
     6361 attgtcgatg tgtttctaaa taaaaaataa ttatttttac taagaatata ggaggagttt
     6421 atatgaataa aagaagatgg attgctgtgg gagtagcgtg cggattattg ttactatcca
     6481 ttatcgtcgc gctaattcca ggaaaagata aagaggaagc atcaaataca accctaacag
     6541 gaataaataa gattttttac ggatctaacg aaatcacaga agaaacgtta gaagaaggcg
     6601 catcaaataa aaagattgtc aaactatccg tcaatggagt tattgcagat acaggtgaaa
     6661 gcaatctttt tagtagggaa caatataacc atcagaattt tttaacacag ctaaagaaaa
     6721 ttcaagaaga caaagcggtt aagggagtgt tattagaagt aaattcacct gggggaggaa
     6781 tatatgaaag tgctgagatt gctaaagaaa tggcaaaaat taaaaagcta gatattccta
     6841 tctatactgc atttaaaaat acagcggcaa gtggagggta ctacatttct gctggttcag
     6901 ataaaatatt tgctacggaa gaaacaacaa ctggatcaat tggtgtcatt atttctggtt
     6961 taaattattc tggcttatta gaaaaacttg gcgtaactga tgcgacgtat aaaagtggtg
     7021 cattaaaaga tatgatgtca cctcaacaca aaccttcaga ggaagagaat aaagttatac
     7081 aggaatttgt gatgagtgct tatgatcgtt ttgtcaatgt tgtagcaaaa ggaagaaata
     7141 tggatactaa tgcagtgaaa gaacttgcgg atggaagaat ttatgatggt aaccaagcag
     7201 tagaaaatgg gttggtggat caaattggtt actctgaaga tgcattagat tccctaaaaa
     7261 aagagaagaa attaactgat gcgacggtta tagaatataa aaatgacaca actggctttg
     7321 cttcttcttg gttaggtaat aagattgctg aatggcaagg gctaaaggca actacatcag
     7381 atcgtgtaat ttcagttttt gaaaaacttg gattttctga tactccaaaa ccaatgtatt
     7441 actatggagg gctataaaat gaataagaag agtggcattg aaactttgcc taattatgta
     7501 tttgcgggat tttggttacg attttttgct tttcttattg atttaatttg tattagcatc
     7561 atcacccatg ctactatagg tctcttttac aaaatcatgg gactggaatc tgacaaatcc
     7621 attattactt tttatggtat agcgtcatta gctatttatt taacctattt tgtacttttg
     7681 actaagctga attcaggaca aacaattggg aaaatgattt ttgggataag agtagtgagc
     7741 tttgaggaaa aagagctaag ttggcaaact atctttattc gtgagtgtat atgtaggttt
     7801 atattaaaaa attttgtatt tcaaattggg tatttggtgg ctgctttcac gcccaagaaa
     7861 caacatattg gggattattt ttctaacacg tcagtggttg ttattcgttc attagaagct
     7921 attaactatg gtcaaaaaat ttctaattct aaaacactta atctggagag aattaaatga
     7981 ataagagaaa aacagagttt attttgattt taattagtgg aataacaggg attttatgta
     8041 gtcttttagc attgatgggt ggtatgtttc tatcttctat tgattcaact actttaaata
     8101 ctacagaact aaccagtaag gatatagata tgttagtcaa tctaagcggg aatgttgttt
     8161 tatttggagg aattacccta gttttttcga taatgatttt cataataggt tttttagtaa
     8221 gaaaaaatca aaaaacagtg gtctatggta ttagtttact cattttatct attattccat
     8281 tttttctttt aacattattg tgggtaattc ctggagtttt aggtattatt gcgggattga
     8341 tgcttctaat aagaaaatct taattaggca atgtgtagac aacgtgaaac gattaatcaa
     8401 ttaaaagcta aatacgtttt gagaaatgat aacttgtagt cgatggaaga catgtagata
     8461 aaaaaactac gtgtctttta tttattctaa ttttttcgaa a
//
LOCUS       DAZUMO010000100         8127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000100 DAZUMO010000000
VERSION     DAZUMO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.102"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..154
                     /locus_tag="ACG8ER_002574"
     CDS             71..154
                     /locus_tag="ACG8ER_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS5051102.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            279..1016
                     /gene="erm(B)"
                     /locus_tag="ACG8ER_002575"
     CDS             279..1016
                     /gene="erm(B)"
                     /locus_tag="ACG8ER_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5051103.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1186..1446
                     /locus_tag="ACG8ER_002576"
                     /pseudo
     CDS             1186..1446
                     /locus_tag="ACG8ER_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1609..2274
                     /locus_tag="ACG8ER_002577"
                     /pseudo
     CDS             1609..2274
                     /locus_tag="ACG8ER_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2310..2555
                     /locus_tag="ACG8ER_002578"
     CDS             2310..2555
                     /locus_tag="ACG8ER_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5051104.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2658..3527
                     /locus_tag="ACG8ER_002579"
     CDS             2658..3527
                     /locus_tag="ACG8ER_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5051105.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLLECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3508..4242
                     /locus_tag="ACG8ER_002580"
     CDS             3508..4242
                     /locus_tag="ACG8ER_002580"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5051106.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4275..5183
                     /locus_tag="ACG8ER_002581"
     CDS             4275..5183
                     /locus_tag="ACG8ER_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5051107.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5180..5722
                     /gene="sat4"
                     /locus_tag="ACG8ER_002582"
     CDS             5180..5722
                     /gene="sat4"
                     /locus_tag="ACG8ER_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS5051108.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5815..6609
                     /locus_tag="ACG8ER_002583"
                     /note="aph(3')-IIIa"
     CDS             5815..6609
                     /locus_tag="ACG8ER_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5051109.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6885..7016
                     /locus_tag="ACG8ER_002584"
                     /pseudo
     CDS             6885..7016
                     /locus_tag="ACG8ER_002584"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            7092..7837
                     /locus_tag="ACG8ER_002585"
                     /pseudo
     CDS             7092..7837
                     /locus_tag="ACG8ER_002585"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7948..>8127
                     /locus_tag="ACG8ER_002586"
     CDS             7948..>8127
                     /locus_tag="ACG8ER_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HFS5051110.1"
                     /translation="MKPKIIMHTQISLDGRIKGFDNPEVYYQVAGGIHSDAVLFGSNT
                     VFTAFEKYPAETEADF"
BASE COUNT         2834 a         1239 c         1706 g         2348 t
ORIGIN      
        1 ttttgtgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa
      121 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
      181 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
      241 tatttattta cagatgtaat cacttcagga gtgattacat gaacaaaaat ataaaatatt
      301 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      361 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      421 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      481 cagaaaaatt aaaactgaac actcgtgtca ctttaattca ccaagatatt ctacagtttc
      541 aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat ttaagcacac
      601 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
      661 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      721 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
      781 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      841 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      901 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
      961 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     1021 atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg agataaatta
     1081 ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc tacggggaat
     1141 ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt aggaaaatgt
     1201 cctaagtgtg gcaacaatat tgtattaaaa aaatcgtttt atggttgttc aaattatcct
     1261 gaatgtaagt ttactttagc tgaacatttt agaaagaaaa aactaaccaa aacgaatgta
     1321 aaagaattac tggagggaaa agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag
     1381 ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat ctctttttca
     1441 aaataaacat ggggctgacc caaaagtagc tcctatatga aaaatagagg tctttccatt
     1501 tagatagtag tggaaagacc tctatttatc ttttgaaagt gaatacaaaa ataaagcaca
     1561 ctctgctata atataggtga ttaatccatt tgagagaggt gctttatcat gtatcaaaat
     1621 tataccataa accaactttg tttgcctata gatcttgaaa ttaaattaga agaaaatgat
     1681 tttgctcatg ccattgtgca gtttgttgac tctattcccg atgaagtctt tcttccttat
     1741 tatcaatcta tgggtagacc ccaatatcat cctcgtatgc tactttccat tattctttgt
     1801 gcctatattc aaggggttta ttcgggccgc caaatacaaa atatgctgat agatagtatt
     1861 cggatgcgct acttatctca agagcaattc cctaactttc gtactattaa tcgttttcgc
     1921 gttcatccga ttatgaacga tattctagat cattcatttg ttcaatttcg tgagctactg
     1981 gttcaaagtg gcttaatcag tggcagtgct ctatacattg atggtacaaa aattgaagcg
     2041 gatgcgaata aatactcttt cgtttggaaa aaaagtattt taaagtataa ggatagcctt
     2101 gatcaaaaag cccttgaaaa ctaccaacaa atggtggaaa caaaaatctt acctgagtta
     2161 atagatgatt taaaggatga actttctata gaagagattg aaaggattcg tcaatctttg
     2221 gaagaaaagg aaaatcaatt aattgaagcg attgaccaaa ccgaaacagt agaagggggc
     2281 tgacccatta ttaataagga gtggacaaca tgattgatgt aaatgagcta ttggaagaag
     2341 ctattagaga aactgaaaat ctaaatgatg gcgaagtttt tcttgttaaa gatttattca
     2401 agggatatgt ggggaatagg atacccagaa aggatcgact tctgcttgga acgttatttt
     2461 taaaccatgt tagcaaaatg aatggtaatt taaaggctat tgaaaagact tcatctaatc
     2521 agcagaggta taaaaagaca attgataagt tttaactatc ttttgtgaaa ttaaaaaaat
     2581 tataaatatg ttgagtagaa gcaaaagatt tggcttggaa tttttacagc agtgaatcga
     2641 taaaaggagc atgcttgatg gtagataata ttattaaatc agtagcagag aaattatcct
     2701 ctctgtctta tatagaaggt attgttttag gtggttcacg tgcaaggggc acccatacag
     2761 aggattcgga tatagatatc ggcatctatt acaattcaga atcatttgac ataaatacta
     2821 ttaatcaatt cgctacaaag ctggatgatg agcatagaaa taaccttgtt gtacctcccg
     2881 gagcatgggg tgattggatt aatggcggcg gatggttagt cataaacggg tatcatgtgg
     2941 atttaatttt acgtgatatt aaacgtgtgg aacaaataat gaaagataca gagcacggaa
     3001 ttgttactgc caattatcag actgggcatc cccatggtta tattagtgca atgtatcgag
     3061 gagaattagc gattagcaaa atactatatg ctaagaatga aagcttatgc gaattaaaaa
     3121 aacaggcaga aacttatccc aatgctttgc agaaaagttt agttaacttt tttatgtttg
     3181 aagcagggtt ctctttaatg tttgtaaaag caaattcggg aacagacgat aaatattata
     3241 ttgcgggtca tgtttttcgt atagtttcat gtttaaatca agtgttattt gcatgtaata
     3301 atgcttattg tatcaacgaa aagaaagcta taaaactgct tgaaactttt gaacataaac
     3361 ctgaaaaata taccgagaag gtaaatcata tttttgaagt actcggtatc tcacttttgg
     3421 aatgctacga catgaccgag aagctttata atgaagtgaa tgaaattgta tcggagataa
     3481 ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt
     3541 tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg
     3601 gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc
     3661 ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt
     3721 gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa
     3781 tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta
     3841 agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg
     3901 atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat
     3961 ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat
     4021 tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat
     4081 agaacactga ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt
     4141 gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga
     4201 cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa
     4261 cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag
     4321 aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac
     4381 ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta
     4441 tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg
     4501 atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt
     4561 acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg
     4621 ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac atagttccga
     4681 ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg
     4741 aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg
     4801 ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg
     4861 tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt
     4921 atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa
     4981 acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg
     5041 cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg
     5101 acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata
     5161 tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga
     5221 taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa
     5281 ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga
     5341 ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat
     5401 atatctttac taccaagacg ataaatgcgt cggaaaagtt aaactgcgaa aaaattggaa
     5461 ccggtacgct tatatagaag atatcgccgt atgtaaggat ttcagggggc aaggcatagg
     5521 cagcgcgctt atcaatatat ctatagaatg ggcaaagcat aaaaacttgc atggactaat
     5581 gcttgaaacc caggacaata accttatagc ttgtaaattc tatcataatt gtggtttcaa
     5641 aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt
     5701 tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata
     5761 attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct
     5821 aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat
     5881 acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat
     5941 ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac
     6001 atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat
     6061 gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat
     6121 gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt
     6181 cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa
     6241 ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac
     6301 actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag
     6361 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa
     6421 gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc
     6481 ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt
     6541 gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa
     6601 ttgttttagt acctagattt agatgtctaa aaagctttaa ctacaagctt tttagacatc
     6661 taatcttttc tgaagtacat ccgcaactgt ccatactctg atgttttata tcttttctaa
     6721 aagttcgcta gatagagttc tatattagag atgtaaagag ttttggtgaa gaggtttgtg
     6781 agtatggctt ttataaccag ggtgcaatga gaagcactaa cagcagctag cttaagaacg
     6841 cataggatat actttttatg gaagtcacag aggaggaata aaatttgaac aggattaaca
     6901 gagtaacctc tattcttatt cagctgcaat caaaaaaaat aattcctgcc aaagaaattg
     6961 cacagcgatt taatataagc ttaaggacag tttacagaga tatccggaca cttgaagaag
     7021 ccggaatacc aattggatct gaggccggaa aaggatattt tcttgtagag ggcttcctac
     7081 ttccgccggt aatgtttaca gcggcggaag tgggtgcatt gattacagca gggaaatttt
     7141 taaattgcca tggagatgaa tcatttataa aggattttga ttcagctatg tataaaatca
     7201 aatctatttt gaagcatggt gaaaaaaact atgcacagga gttggagaac agtattaacg
     7261 tgtacagcac atctggacag aaaaatacat tggcggataa cgttattgca gcaattcaga
     7321 ctgcaatctg caataaaagg gtaatatcaa ttcaatatcc tgcatcagga gggcaagaac
     7381 cggaaagcag aatgatagag cctatatcac tggggtttta tgaacagaac tggtacttaa
     7441 tcggttttgc aggctaagaa atgaatatag gaattttcgt gtggatcgga ttaaaagcat
     7501 ttgcatagaa gaagagctct gtcaaagcca tgatggctct ttggaacaaa tattaaaaca
     7561 aatgctttca tataaaaaac tatataatgt aattcttaga gctgaaaaag gggagacata
     7621 caattctatc aagaacaggt attctctcgg cttccttgaa gaaaccgatt tgggaagtaa
     7681 aatggaaatc gaattccaga cagattcctt tgagatattg agcaagcagc tgattgaata
     7741 tggatcaggc attgaaatag tccaacctga tgaattaaaa tgcataaccc gtaaacacct
     7801 ggcacaaatt acgaatcact gcctaaactt gatttagaga aagcgattgg tctgttatgt
     7861 attttttgta aggtttcata tcccctgctg acatacagct gtcagtgtat tatgatattc
     7921 tactatcaaa aaggaggatt gaaaaatatg aaaccaaaga taattatgca cacacaaatt
     7981 agccttgatg gccgcattaa aggctttgat aatccggaag tttactacca ggttgccggg
     8041 ggaattcatt cggatgcggt gctgtttggg tccaatactg tttttactgc atttgaaaaa
     8101 tatcctgctg aaacagaggc tgatttt
//
LOCUS       DAZUMO010000101         8085 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000101 DAZUMO010000000
VERSION     DAZUMO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.103"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..942
                     /gene="msrA"
                     /locus_tag="ACG8ER_002587"
     CDS             109..942
                     /gene="msrA"
                     /locus_tag="ACG8ER_002587"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5051111.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1022..2011
                     /locus_tag="ACG8ER_002588"
     CDS             1022..2011
                     /locus_tag="ACG8ER_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5051112.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2300..2998
                     /locus_tag="ACG8ER_002589"
     CDS             2300..2998
                     /locus_tag="ACG8ER_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS5051113.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3148..4464
                     /locus_tag="ACG8ER_002590"
     CDS             3148..4464
                     /locus_tag="ACG8ER_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5051114.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            4481..5896
                     /locus_tag="ACG8ER_002591"
     CDS             4481..5896
                     /locus_tag="ACG8ER_002591"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5051115.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            6360..6680
                     /locus_tag="ACG8ER_002592"
     CDS             6360..6680
                     /locus_tag="ACG8ER_002592"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5051116.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            6706..7023
                     /locus_tag="ACG8ER_002593"
     CDS             6706..7023
                     /locus_tag="ACG8ER_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5051117.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            7052..>8085
                     /locus_tag="ACG8ER_002594"
     CDS             7052..>8085
                     /locus_tag="ACG8ER_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5051118.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
BASE COUNT         2662 a         1316 c         1717 g         2390 t
ORIGIN      
        1 tttatacgaa aaatctgtag ggggatgaag taatcgctta tttcctcact ttatgataaa
       61 ttacagctaa agtcactagc ttgtcttctt cggaaaggag gagaatctat ggatgaggaa
      121 caaatattca ttcaaacact ttataatctg attttgaatc cgaatacacg agattgggaa
      181 cgaaaagtat tgattcagac aaagaatgac ataagagaaa atatatccgt taaagaacag
      241 ttatcaaaac tagaagcaac actacgtcca ttagctatcc gaatgaatct gacgcctgat
      301 gtaatggact tttatttatt gattacggaa ggatttgata aggagcaaaa atatgacttt
      361 tccaagcatg ccatgcaaga tgctgactat caagaacgtg cagtatttgc agggggatgt
      421 ttctggtgta tggtcgagcc gtttgaatcg aaaaaaggga tcctatctgt tttgtcagga
      481 tacacgggcg gacatgttga aaaaccaaat tatgatcaag taagtggtgg ttacacaggt
      541 catgtggaag ctgtggagat catttatgac acaagagaaa taagttatag cgagttgctg
      601 acaatctatt ggcaaattac tgatcccaca gatacatttg gacaatttca agatagaggg
      661 aagcaatatc gtccagtgat tttttatcaa gtcgaacgac aaaaagaatt agcggaacaa
      721 tcaaaacaga aactagacag ttcaggaact ttccaccaac caatcgtcac aaaaatcgaa
      781 ccagccggta cgttttggcc agcagaaaac tatcatcagc aattttacaa aaaacaaccg
      841 aaacgttata aaaaaatcca gcaagcacgt aatcaatttc tgatctatca acgagtaaaa
      901 aacaaatggc aaaaaaatat acgaaaaaat cattttgatt aactcctttt ctttaaaatg
      961 tagtaaacta aaaatagaaa aaagaaagcg ttttaaaaag atttgatgag gaggagaaaa
     1021 attgtatatt gcagatagtg cacgttatga aaaaatggaa tatcgtaaat ccggtcattc
     1081 aggtttgaaa ttaccgattt tatctctcgg attatggcaa aattttggtg attatgatcc
     1141 tatccataat caaagagaga ttttaagagg cgcattcgac atgggaatta cccatttcga
     1201 ccttgcgaat aattatggag gccctgctgg agctgcagag aaaaactttg ggcgtatttt
     1261 tagagaagat tttcaagcct accgtgatga actgattatt tctagcaaag caggttatca
     1321 tatgtgggaa gggccatatg gggaatgggg ctctcgtaaa tccatcattt caagttgtga
     1381 ccaaagtctc caaagaatgg ggctagatta tgttgacatc ttctatcatc atcgcccaga
     1441 ccctgataca cctttggaag aaacagcaga agctttgatg caacttgtaa gacaaggaaa
     1501 agcattatat attggtatat ccaactataa tggagaagat acaaaaaaaa tgactgaaat
     1561 tttaaaaaga aaggaagcac cgttcattat tcatcagatg cgctacaata tgttttctcg
     1621 tgcactacta gaagacgatc tatctccagt attagaagaa gaagggctag gggcaatcac
     1681 gttcagtccg cttgctcaag ggttgctgac aaatcgttat ctacatggta ttcctgaaga
     1741 ttctagagca catcgtaagg aaataccatt cctttcagaa gaacaagttg gatcaactct
     1801 ggaaaaaatc aaagcactgc aaacaatcgc cgtatcacga ggacaatcat tagcgcaaat
     1861 ggcccttgca tggaatttgc gacaaaaatc tgttacaagt gtactggtcg gagcaagtcg
     1921 gttaagtcag ttacaagaaa gtgttcgaat gatggataat ttagatttct caccagaaga
     1981 agaattgaga atcgaccaaa ttctagaata gaaacgatgt aatcaaactt tttatccata
     2041 tattaaaaaa aacaaaagat gacatcaaga atcgaatgaa gatcgtctgt tattgaacat
     2101 catatttttt gtcgttcaaa ataaaaatga tcgaaggcta ggagtgaatt ttatcctagt
     2161 cttttttatg gtaaatatct tcttcgtttg tatgtctatg aacggattaa tcaattttgt
     2221 atacaaaaaa tcatcagatg attcaaacgt gttataataa ttacgaaaaa atacatcaga
     2281 tgaaaaggag gtatgtggtt tggaagaaaa taaaacccta gtagaagtaa ctactgacaa
     2341 gatccttaga tatttaaagg aaatgcagtt gcaagtagga gataaactgc cgaatgaata
     2401 tgaattgtcc gattatttag aggtggggcg aagcacggtg cgagaagctg tgcgggctct
     2461 agcttccagg aatatccttg aagttcggca aggagcgggg acatttgtca gtgataagcg
     2521 agggattgcc aatgacccac tcggtttttc tttgattgaa gatcaggatc ggatgatacg
     2581 agacttattt gaactgagat atttattgga gcctaagatg gcaggtttgg cagcggaata
     2641 tgcaacgaag aatcaaatcg tccaaatgaa aaaattaacg caaaagattg aaacttcttt
     2701 tcgagaaggc acacaagaac atgttcaatt agacatcgat tttcatacga tgattgcaga
     2761 agctagcgga aacgtagcct tgctgcatat tatcccaatc attaatgaat caattgcttt
     2821 gtttaataaa aattatgata tccagcatct gaaaatcgag acgatcgata ttcatacaga
     2881 gatcgttcag gccatcgaaa ggcggaatgc acttgctgcg tttgatgcaa tgactgtcca
     2941 tatggcaata aatcggagag aattacagaa aataatagga gaaaaagaaa ctaattgaca
     3001 gttcatctga tgtattttat gataaataca tcagatgaat ttttgtttta tatttggtaa
     3061 aaaagaggag taaatagttt ctgttcatcg aaatgtaagc gctgactaat agacaaaaag
     3121 agatgacgtc aaggagaaga agcaggaatg aggggatctg gaatgagttt tatggataaa
     3181 ttagccaatg ttttagggaa cgtggcaacg agaatcaata atttaaggta tatcatggtg
     3241 ataaaaaacg catttgctgc attgattccg gtaattatta cgggtgcttt tggcacattg
     3301 ttttcagcaa tggtctttga ctcggaaaat ggcttagcac agataagttt cttgcgtttt
     3361 ttagaagaat taaagcctat cgcatctgcc atcagctatg tcacactaag tttcttgacc
     3421 atctatgctg tttttctgat agggatcgaa ttagccaagc tgaataaagt ggatggtgta
     3481 ttccctggta tcatcgcagt gatgtcttat ttatctgtta atccaactgt ttatgaatta
     3541 gtgaatgaaa atcagactgt tatagtagaa aacgtattgg ctaaacaata tacagatacg
     3601 aaagggttat ttttgggaat gttggtctcc atcctttcta ttgaacttta ttgctggttg
     3661 aggaaacaga agaaactaca gctgagaatg cctgataccg tgccaacgaa tgtttctgct
     3721 agtttttctg ctttattccc gacgattctg actgtatcgg gtatcgcggc tgcgggattt
     3781 atcatcaaag aacttacagg aatgtacctt tatgatatca tttacaatgt cgtacaacgc
     3841 ccattagaag gtgtcgtaca agggttaccg ggtattttgc tattgatgtt tattgcgcaa
     3901 gtcttttggg tgattggtat ccatggaaat caaatggtga agccgatacg ggaaccgttg
     3961 cttttagcgg caattgctgt aaatacggaa gcttttgaag ctggaaaaga agtaccgaac
     4021 attattacta tgccattttg ggatatgtat atgagtatgg gcgggtctgg agtcacgatc
     4081 ggtttactga ttgcagtatt gttagttgga aaacgggaag atatgaaaca aatcaccaag
     4141 ttatcccttg ctcctgggat cttcaacatc aatgaaccag ttatttttgg tatgccgatc
     4201 atgctgaatc caattttagc tattccgttt atcttaacgc ctcttatcac tggaacgatt
     4261 ggttattttg caaccgcttt aggatttgcg gcgaaagcag ttgtaatggt cccttggcca
     4321 atgccgccga tcgtcaatgc ttatctggca acagcaggaa atatcggtgc agttattacg
     4381 caacttgttt gtattattgt ttctattttg atctatctgc catttgtaaa agtagcaaat
     4441 cgtgtggaaa atgatgggat ctaaaaaaca ggaggaaatc atgaaaatta ctattccaaa
     4501 aaattttatt cttggtgctg catcttctgc ttggcaaacg gaaggttgga aaggaaaaaa
     4561 agaaggacag gattcgttta tagatagctg gtataaaaat gaacattttg tttggcataa
     4621 tgggtatggc ccagccgtag caactaattt tatggaacaa taccagcagg atgtggatct
     4681 gatgaaatcc atccatctga cacattatcg gacttctatc aattgggctc gtttttttac
     4741 tgattatgag aactgtatag tagatgaaga atatgctcag catataagtg aaatgatcga
     4801 ctctttgatc gaagcagacg tagaaccgat gatttgtttg gaacattatg aagtcccagc
     4861 ttttcttttg gaaaaatatg atggctggtc ttcaagaaaa gtagttgatt tttatgctaa
     4921 gtatgctcaa attgcctttg atcgttacgg tgatagggta aaaaactggt ttacattcaa
     4981 cgaaccaatc gttccccaaa cgcgttgcta tttggatgct gttcgctggc cgcatgaaca
     5041 aaacacaaaa aagtggatga tgtggaatta tcataaagtt ttagcaacgg ctaaagcagt
     5101 agagatttac cgcagcggaa attatggtgg aaggattggc tgtattttaa atcctgaaat
     5161 ggtctatgct cgatccaatt ctccagaaga ccaaaaagct gcttatatgt atgatttgtt
     5221 ttataatcga gtattttttg atccgatggt aaaaggatac tacacagaag agttcctgga
     5281 gttgtgtcaa aaacaatcga tttatttcga tcctgcaaca gaggatttag aaacgattca
     5341 gcatcataca gtagatttcc ttggaatcaa tcaatattat cctaaacgcg ttcgcgcccc
     5401 tcgttatcaa tggaatgaag agacaccttt ccatcctgaa cgatattatg aagtctttga
     5461 tttacctggg aagaaaatga ataattccag gggatgggaa atctatccga aaatcatgta
     5521 tgatatagcc atgtatctaa aagaaaatta tcataatatc ccttggctga taacagaaaa
     5581 cggtatggga agagaatatg aagaacagta tatggatgag aaaggaatcg tgcaagatga
     5641 ttaccgtatc gaatttatcg gacagcattt gcattacttg atcaaagcaa cagaagaagg
     5701 cgcaaattgc gaaggctata tgttatgggc attcactgac tgtgtgtcac caatgaatgc
     5761 atttaagaat cgatatggat tagttcgaat cgaattgaac gatgaacgaa gcagaacatt
     5821 gaaaaaatct gcttcgtggt atcagacttt gattgataag agagaattgg atttgcctga
     5881 cgaaatctat aaataaatag aagttttttg tagatgttca ttgtatttac tggttatttg
     5941 aaataaaaga ggttgtgaat ccagcgtttt gagctaatac ccagggtcag ctgattgaac
     6001 accgtttatt gagaggttgt gaaaaagctg tcctgcatca agtaataaga atagcaaagt
     6061 aaaaacaaca tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg
     6121 aataccgttt atttgaaaaa agttgtgaca agttttgtgt cacaactttt tttgtgcacg
     6181 ttaaaagaaa aaaggtaata aaaattaatt tgtgtgttta gtgtgcgctt tcttttttag
     6241 tgtgtatgca acttggtata gaccttaaat gtgatgatat caacttttta aagcgctttt
     6301 aaagttggca cgctacttgc ttatatacaa gtgtaagtat agaaaggaac gtgtttaaaa
     6361 tggaaaagaa aacaatcatg cttgtatgtt cagctggaat gagtacaagt ctcctagtca
     6421 caaaaatgca aaaggcagca gaagcaaaag gaatggaagc agacatcttt gctgtatctg
     6481 cttcagatgc ggataatcaa ctagaatcta aaaatgtaga cgttttactt ttaggaccgc
     6541 aagtacgctt tatgcaaaat caatttgctg aaaagctagc acctaaaggc attcctttag
     6601 atgtaatcaa tatggctgac tatggcatga tgaatggtga aaaagtactc gaccaagcag
     6661 aaaaactaat aaacaaataa taaaaattat cggaggatac aataaatgga agatcaaaaa
     6721 aatctagaag ccatcatggg tctgatcatg tttggcggaa atgcaaaaag cgatgcaatg
     6781 gaagcgattg ctgctgcgaa aaaaggtgat ttcactttag cagatgaaaa aatcaaagat
     6841 gcagaggaat ctttagtcca agcacatcac tctcaaacag agatgctgac acaagaagca
     6901 caaggaaatc acgttgaagt cacgcttttg acggtccaca gtcaagatca tctgatgaca
     6961 tctatagctt ttacagattt agcaaaagag atcattgatc tgtatcgtcg attgaacgcg
     7021 taatctacag taaagaaaag agggaagatt tatggataaa tttgttgcat ttatggagaa
     7081 acattttata cctgtagcat caaaaattgg tgcacaacgg catttagttg cgattcggga
     7141 ctcatttatg gtgagtatgc cgttaatgat cttaggagca ttagcagtaa tgatcaataa
     7201 cttaccgatt cccgggttcc aagaattgat gaactcaatt tttggtggtg aatcatggaa
     7261 aggattcggg gctgctgctt ggaacggtac atttgccatt ctttctgtct tgattgcttt
     7321 cttgcttgct tatcatttag ctaacggcta ccgaaaagac ggtgtttccg caggtgtgat
     7381 ttctttaggc tctttcttcg ctttaggcgg agcattaggt atgagctcaa acggtttgtt
     7441 tattgctatt attgttggga ttatttcaac agaaatcttt gtacgtttaa gcggaaatga
     7501 aaaattgatc atcaaaatgc ctgatggtgt gccaccggca gttgcaaaag catttgcttc
     7561 attactacca gcaatgatca ctatctctgc gtttgcttta gttgctgcaa tttttgctgc
     7621 attcggtgtt gatgatatcg taggttcttt ctatacagtg gtccaagaac catttatggg
     7681 acttgcaaac tcttatccat ctgccttact gcttgcattt atcacaccat tcttatggtt
     7741 ctttggttta cacggagcga atatggtcga tcctttgatg caaacaatca atgcgccagc
     7801 cattgaagca aacgttaatg caatcgcagc tggacatagc gcaccgttta tcgtgaacaa
     7861 acctttcttt gacagctttg tcaacttagg aggaacaggt gcaactttag gtttactgtt
     7921 agctatttat ttagtaggac gtaaaaacaa accatatatg gttgtaacaa acttgtcgat
     7981 tgcgccaggt gtcttcaata ttaatgaacc aacgatgttt gggttaccta tcgtgttgaa
     8041 cccgatcatg ttcattccat ttattttgac accaatggtc ttagt
//
LOCUS       DAZUMO010000102         8021 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000102 DAZUMO010000000
VERSION     DAZUMO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.104"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..2828
                     /locus_tag="ACG8ER_002595"
     CDS             51..2828
                     /locus_tag="ACG8ER_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5051119.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2909..3328
                     /locus_tag="ACG8ER_002596"
     CDS             2909..3328
                     /locus_tag="ACG8ER_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5051120.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3340..3810
                     /locus_tag="ACG8ER_002597"
     CDS             3340..3810
                     /locus_tag="ACG8ER_002597"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5051121.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3827..4606
                     /locus_tag="ACG8ER_002598"
     CDS             3827..4606
                     /locus_tag="ACG8ER_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5051122.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4593..5414
                     /locus_tag="ACG8ER_002599"
     CDS             4593..5414
                     /locus_tag="ACG8ER_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5051123.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5431..6480
                     /locus_tag="ACG8ER_002600"
     CDS             5431..6480
                     /locus_tag="ACG8ER_002600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5051124.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6493..7497
                     /locus_tag="ACG8ER_002601"
     CDS             6493..7497
                     /locus_tag="ACG8ER_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5051125.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2654 a         1353 c         1683 g         2331 t
ORIGIN      
        1 attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg gtgaataagt
       61 tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc gatttagaac
      121 atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta aaaagaaaca
      181 cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa atcaattctc
      241 gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag ttacgcacga
      301 atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg caaccaaaag
      361 acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc gaacaaatca
      421 aaacagctct ttactatcca gatggcggat taccactttt gattacaggc gaaagtggca
      481 caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat gatctgctag
      541 aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat cctgaacttt
      601 tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa gaaaataaaa
      661 aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta catcgcttaa
      721 caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt tatcgtatgg
      781 gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg actgaagaga
      841 ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc gaactacctg
      901 cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt tttctcgaag
      961 aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca cttttaacaa
     1021 gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa
     1081 aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat catttgcaaa
     1141 aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc tatattacgc
     1201 aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag cgaatcgtta
     1261 aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta tattcttgcg
     1321 aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt gagaccagta
     1381 gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag acctttcgac
     1441 aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc ttgagttact
     1501 atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt atccaactaa
     1561 tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac tatgtccaac
     1621 gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg gatcggattt
     1681 tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga
     1741 tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta aatcgtttat
     1801 tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca cagcagattg
     1861 cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg atcatattag
     1921 tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta tccgtaccgc
     1981 ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa tgcctgcaaa
     2041 aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag ccagaatggc
     2101 gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc ttcactggaa
     2161 tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg gcttgtcagt
     2221 tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt gacccattat
     2281 tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc accaatatcg
     2341 actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta gctgaatggt
     2401 tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc attcgtaatt
     2461 tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag gtgatgggag
     2521 aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc agcaatccca
     2581 aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc cgacaaatgc
     2641 caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg
     2701 ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc cccaattctg
     2761 agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg atagaagaag
     2821 aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc ttttttagta
     2881 tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag cttcccatgg
     2941 aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa atcattctat
     3001 tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag taaggcagct
     3061 ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg gcggaagtgt
     3121 caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg ccggattgaa
     3181 tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga caactgtttt
     3241 gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg agcggctcaa
     3301 tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt gacaagagtc
     3361 gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat aggcgcagac
     3421 tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac aacgatcaaa
     3481 ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc gattgccgcg
     3541 ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc gatcgaggat
     3601 gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg agggatcaag
     3661 gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga agaggaggaa
     3721 cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt accagcagat
     3781 aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga ttgtacaagc
     3841 agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag ggacatcgtt
     3901 gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac
     3961 tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct cagtaggtgc
     4021 ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg ctattacagc
     4081 tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt tgacattgat
     4141 attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag cagacaatta
     4201 tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg gtatcggtct
     4261 ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca atacgatcgg
     4321 aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt
     4381 gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc
     4441 atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga ccggagtagc
     4501 tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc gtcaactacc
     4561 acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc aaaaacagca
     4621 aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg gagttttctt
     4681 ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg attttgaaaa
     4741 agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta gagtttttta
     4801 acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg gaggaagaaa
     4861 ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca tctttgatgg
     4921 gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg attgccacag
     4981 gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg ttcattttga
     5041 ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga tatcgcttag
     5101 gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg acattaggcg
     5161 cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc accatcaata
     5221 ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata
     5281 tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta ttgaagaaag
     5341 aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt ggttcgtgga
     5401 tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta tatctatgaa
     5461 gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag agaacatatg
     5521 aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc ttgtttagca
     5581 gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga acctttttat
     5641 tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt gtcacaaagc
     5701 gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg gttacctgtt
     5761 gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca aattattgat
     5821 ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc gactgtttta
     5881 cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag caaaaaaata
     5941 gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc gattattcaa
     6001 aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca gcgtttcgtc
     6061 accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa attgactgaa
     6121 acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg cccatacctg
     6181 gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa taaggaacgt
     6241 tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac cattaccaca
     6301 aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga atatctgtct
     6361 gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt agctaaaggt
     6421 attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag taaattataa
     6481 gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca
     6541 ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga aaaatttgac
     6601 gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc tgtcacttat
     6661 atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata ttacactact
     6721 ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac cggtacaact
     6781 caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact gattggattt
     6841 atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac ttatccagct
     6901 cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta tttgaatgga
     6961 gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc aacagggtta
     7021 gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa acatcgacgt
     7081 gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta ttcatatgcc
     7141 atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc tgccgagttt
     7201 cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt tttaggcgaa
     7261 gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat
     7321 tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa atatcgcgga
     7381 cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc tcatgtggag
     7441 cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga ttattaggat
     7501 agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc cctttatttt
     7561 tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc tacagttcaa
     7621 cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg caatagtact
     7681 atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc gccagtttac
     7741 atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa ccgtcaacag
     7801 agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg tctcttatgt
     7861 attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa tgctggtagt
     7921 aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg taaaaataaa
     7981 atgtagacta ttgtaacata tcattgatta tttcaagtaa a
//
LOCUS       DAZUMO010000103         7915 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000103 DAZUMO010000000
VERSION     DAZUMO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.105"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..937
                     /gene="truB"
                     /locus_tag="ACG8ER_002602"
     CDS             14..937
                     /gene="truB"
                     /locus_tag="ACG8ER_002602"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS5051126.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            941..1882
                     /gene="ribF"
                     /locus_tag="ACG8ER_002603"
     CDS             941..1882
                     /gene="ribF"
                     /locus_tag="ACG8ER_002603"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS5051127.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2277..3062
                     /locus_tag="ACG8ER_002604"
     CDS             2277..3062
                     /locus_tag="ACG8ER_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5051128.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3075..3926
                     /locus_tag="ACG8ER_002605"
     CDS             3075..3926
                     /locus_tag="ACG8ER_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5051129.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4050..4502)
                     /locus_tag="ACG8ER_002606"
     CDS             complement(4050..4502)
                     /locus_tag="ACG8ER_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051130.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(4967..5335)
                     /locus_tag="ACG8ER_002607"
     CDS             complement(4967..5335)
                     /locus_tag="ACG8ER_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS5051131.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5485..5967
                     /locus_tag="ACG8ER_002608"
     CDS             5485..5967
                     /locus_tag="ACG8ER_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS5051132.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6004..7014
                     /locus_tag="ACG8ER_002609"
     CDS             6004..7014
                     /locus_tag="ACG8ER_002609"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS5051133.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7129..7842)
                     /locus_tag="ACG8ER_002610"
     CDS             complement(7129..7842)
                     /locus_tag="ACG8ER_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051134.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2655 a         1291 c         1624 g         2345 t
ORIGIN      
        1 atggagaaaa gacatggacg ggttattaac tttatggaaa gaacgcggaa tgacaagtca
       61 tgattgcgta ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac
      121 gcttgaccca gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat
      181 cgaatatttg acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac
      241 aacgacggaa gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc
      301 tgaagaaaaa atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc
      361 aatgtactca gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga
      421 aacagtggag cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt
      481 gacaagagac gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg
      541 aacctatgtc cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat
      601 gtctgatctg acccgaacag caagtgccgg tatgtcccag aaagatgctc tgacattaga
      661 agaagtagct caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa
      721 aggagtgaaa cgattcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa
      781 cggtatgaga cttaattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc
      841 tttgttttat aaaggcaaag ttgtcagtat ctatcagcca aacccgaaag aaaaaaacaa
      901 attaaaacct agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag
      961 acatccttac caatcagaac aaattccaga agacgatgtc gttttagtac taggattttt
     1021 tgacggcgtg catattggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa
     1081 agaaggattg aaattagcat tgatgacttt caatcagcat ccatcgattg tctttaaaaa
     1141 gatcgatcct agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc
     1201 cgctttgggt atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc
     1261 accgcaggaa tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg
     1321 ttttgattat acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc
     1381 caaaggaaga ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag
     1441 ctctacccgt attcgaggat tactagacag cggacaagta aaagaagcag caaaactatt
     1501 aggttgcaca tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact
     1561 aggattccct acagcaaatg tcaaagtaaa gagcacagtt catttgccta aagaaggtgt
     1621 ttatgtatcg gaaataaaaa ttggggaaat ttggtatcca gccatgggtt caattggaca
     1681 taatgataca ttcggtgaag gacgggagct gactgtagaa ttgtatattt tggactttaa
     1741 tcaagatatc tatggtgaac atgtcgcagt acgctggaat caatttttaa gagatcaagt
     1801 gaaattttct agtgtagagg aattgattga acaactcaaa gaggatgaac aagcaacagc
     1861 tgaatatttt tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag
     1921 caatttcaat aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt
     1981 cacagccttt ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga
     2041 aatttcacaa aaatttgaga agtaattttt gtaaattact tcttatttct tgaagctggc
     2101 cacttctgtc acaacctctt tctttataga aataaaagga cactagcaat tcatgaaaac
     2161 gattgctaaa gcgtatcatt tgctttagac tatatatctt ggtgtacact cactttaaga
     2221 cggaaataaa agttatccgt aataacaggt ttaggtatga aaaaggagtg ggtactatgt
     2281 ataaggaatt agaaaacaaa gtcgcagtag taactggtgg ctcaaagggg attggaacag
     2341 cgattgctca acgattcggg aaagaaaaaa tgaatgtagt tgtcaactat aactcggata
     2401 aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc aggcggaaaa gcagtctctg
     2461 tacaagcaga tgtcagttct gaagaaggaa tacagcgttt acttgatacg gctttagaaa
     2521 attttgggac actggacgtt tggattaata atgctgggat ggaaaatcag catccaaccc
     2581 atgaacttag ccttgaagat tgggaaagag tattaaaagt caatttgact ggcgttttta
     2641 tcggaacgaa agcggcttta aattacttta cagagaatga taaaaaagga aatatcatca
     2701 atatgtcttc tgttcatgaa caaatcccat ggccaacgtt tgctcattac gctgcttcaa
     2761 aaggcggagt aaaactattc accgaaacgg tagcaatgga gtacgcacca aagggaatca
     2821 gaatcaatag tataggacct ggtgctatcc gtacacctat taatgcggca aaatttgatg
     2881 atcctgaaca aaaagaactc ttggaaagta tgatcccact aggacgcgta ggtgaaccaa
     2941 aagaaatagc ggcgacagct gcatggttgg cttcagacga atccagttat gttacaggta
     3001 ttacactatt tgcagatggt gggatgactt tatatccttc tttccaaggt ggaaaaggat
     3061 aagggtgaat aaaaatgggt ttattgattg cattgatccc ggcagttggg tgggggatcc
     3121 agccactgat tttgaaaaag attggtggca gaccaacaaa tgagatatta ggaactggta
     3181 ttggagccgt gctcgttggc ttacttgttc aattattcgc ttctccagct ggtatttcag
     3241 tgactacatt tttgattagt ttactttctg gtgcattctg ggtcatcgga caaacaggac
     3301 agtatacatc tttcaacttg attggtgttt ctaaaacgat gccaatttcg actgcattac
     3361 agttagtcgg aacctcattg attggtgtct tcgcttttgg agaatggtct ggaagtatgg
     3421 gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt tggttctgct ttaacggcaa
     3481 tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg aatcagtcta ttagcatcaa
     3541 caagtattgg ttattgggtt tacagtgcat tgcccaaact agttgatgca agcggacttt
     3601 ccattttctt tccgcaaatg atcggtgtct ttttaggggc agtgatttat gtgattttcc
     3661 gaaaaccaac cgcattttct gaagacaaga gttggaaagc aacaatagtt ggagccatct
     3721 tcagtgtgtc agcattggct tatatttttt cagcaaatga aaatggggtt gctactgcat
     3781 atatcattac acagctaaac gtagttatct ctacattagg cggtatgtac atcctgcatg
     3841 aaaataagac accaaaagaa aaaaaattaa cgattatcgg attattgctg atcgtagtag
     3901 gaagcatggt cacggtcttt atttgaggat aaagatggta aaatctcatt tattaaaaaa
     3961 atatttgttt gaaccaaaca gtaagtactt ataaaaaaat agcgctagaa tttccaacat
     4021 ctctggaatt tctagcgctt tttaattgtt taagatacaa gtgaaatatc tgtctgcatc
     4081 aataattttt cattgatcca ttgactaaaa tttacgacat gattgaagtt accaccgatt
     4141 agtatctccg catgattgtc tttataaaga tcgtattgta aaagacggtc ttttttattt
     4201 gcgtaaacat cgttatggta ctggactaat tgttctcgga tttgattgat tgctgtaaaa
     4261 tcacgatact gaaggttgaa acgcatcgtg attggtgcat ttagcatgaa tcgctgcatt
     4321 tcttttgctg cttcctctgc ttgatctccg tataggaact caaaaagatt acgttcagaa
     4381 aatttccatt gcccgccaac ctttgttcct tttagttttc cttcatgaag ataatttctg
     4441 atagaacgtg tagtaacacc taattgttcg gctaattcat ttactgagta aaaatctttc
     4501 aatgttcacc ctcctatgtg aatggttcta ctgacttagt tgatatttgc ttaatcacga
     4561 aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa tcaagtcaga aaattctaac
     4621 ttttctttct ctaaagaaat tcgttctctc ctaaagtgat attatattta aatatacctg
     4681 aaaataatgg gcagtattat gaaagatcac tgatattttc tcgtattttt tccttattca
     4741 aaagttttgc aggaaaattg ctgaaaaaat aattttacta agtggaaaat tcaaagccat
     4801 tcaatcagtg atttttttca gcacaagaaa gactccttat aaaaatagca gatactttgt
     4861 caattaagtg tttaaccagg gatgataaaa aggataggat tgaacaaaac ctgtataagc
     4921 tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat aaagctttat cgccaaaatt
     4981 tatcatgacc ttttttatct tcaatattaa atgaaatcaa ttgttcaatc aattcccagt
     5041 tgattacact tttccaagga actttgatga cgttatccgt atatacgtag cctgcttctt
     5101 ggatattttt ttcaagggca cgaatcgctg ctttttccgg tgcgatagaa aaatgatttt
     5161 tagcaaaact aaatgcaata ataaaggtac catggtcagt aaacataggt tggttccatt
     5221 ttacagttgt gtctagctgg ggatacgttt ctgttatctt agcaaaaagg tctttgatct
     5281 tttgttgatg ttctttttct ttgatctgat tgatgatcgg ctgaaatttt tccattctaa
     5341 aacgcctcca ttaagtggtt tgatttattt taccagaaaa acaatagtta tgtataaaaa
     5401 acgaacaaaa cacaagtaat gttgtaaccg ctatatttat atgataagat gtctcaaata
     5461 ataaaataga aaagagacat aaaaatgatt gacttggcaa agaaattctt acacgaaaat
     5521 atatattttg agttgtatca tcatcgagcc atttatacaa atgaagatgc tttggtaata
     5581 aagaaagaac aaggatttac aggtacagag accaaaagct tattcttaaa agataaaaaa
     5641 gataataact acgtatatct gacttttact acgaaacaaa cggatttcaa agcattaggg
     5701 aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac gcatggaaga acaaacggga
     5761 caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac agattccgat tattattgat
     5821 gaagagctct tatcaaatga aaaacttgtt tttgcaccag gtagaccaga tcaaacgatg
     5881 gtcatccaaa cggttgactt aaacaaaatc atccagctac ttgagatgcc gatatttttg
     5941 atgccggcaa acgaaaaaaa caattaacca agtaaaatag tgcatacttg gaggtgaagc
     6001 aacatgataa gttatccatt attagaaaga aatgcaacga ttggggttac tgcaccatcc
     6061 tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag ctttaaagag aatggaagaa
     6121 aaatctttcc aagtagttac tggaaagacg ctttggacgc aagataagga aaaatcaggt
     6181 ccagcaagaa aacgagcaga tgaattcaat caaatgatgc aagatgaaaa tatccaaatg
     6241 atctttccgc cttggggagg agaattattg attgagattc tcgattttgt cgattttgat
     6301 gctctacaac caaaatggat agtaggttat tcagatatta gtacgttgct acttgctgtc
     6361 acattaaaaa cagggatagc cactgcacat ggaaccaacc ttattgattt aagaggagag
     6421 tacttggatg aaacgacagc aatgtgggaa agtgtactga aaacaagaga aggagaatcg
     6481 attctacagc aaccttctga aaagtatcaa aaagagtggc cccatgagca ttcatcatct
     6541 tctgttttcc atctcactga accaacaaat tggcaatcag tcagcggaga tgcagtaagg
     6601 atgaagggac gattattagg tggctgtatt gatacgatca gtcatttaat cggtacccct
     6661 tatggagatg tccaatcttt taaaagaaag tataatgatg gaaagccgat cttgtggttt
     6721 ctagaaaatt gtgagctatc gtcagttgat ttaagaagat cacttgttca gatgaaactg
     6781 gcaggatggt ttgatggcag ctcggggatc atgttcggta gaagtgcagc agaggatttg
     6841 ttagattata aaatgaagga tgtatatgaa gatctgtcaa aggaattaaa actaccgatc
     6901 ttgtatgata ttgattgtgg tcatgtaccg ccacaactga cattgatcaa tggtgcctat
     6961 gcagaagtag cggtagaaga tagaaaagcg actgttttgc aaacgtttat ttaaaaaatt
     7021 ttttcctgcc ttggagttta tcaaagacca acaaatctaa atagaaaaga aaatgaaaga
     7081 agctgacagt attttgccag cttctttttg tatgatctat tagaaatttt aattatccat
     7141 aactatcaca ttgatcagct gtttcacatc atttggatta cctgtaggaa tagctactac
     7201 agatgcatta tcaataattt cttgagttgt ttctctaatg aacgctggat aagatgtttg
     7261 atctgcaggc atttgccaat gtgaaggaac tggataacta gtgattgtcc cgtcatggtt
     7321 acttgaatac actaaaactc cctggtaact gtatttccca gtgagcataa ccacatcttt
     7381 cggatagact gcactccctg catcataagg ggcgataggt gttcctgcag gataatgaat
     7441 aacatgtagt tcgaaatcac ccgatgtatc tctaaaagta ttacctctaa ttgctagcca
     7501 aacacgtgcg tactcaattt gtagatcaga atatcctgct aacggatcta ttgactgaga
     7561 agactgtgag gaagttgtgt ctattgttga agaagaaata tttgtctcaa ctgtactact
     7621 agatgtagaa gaagtattgg gctctgagac tttactgctt gttgaagatg tattcgtttc
     7681 agtagaagct gatttctgag tacttgattt ttcagctgaa ctggtttgtt tgattgaact
     7741 tttgtctgtg gtcgcagatg gatcactatt ttctttacta ggagacgtac atcctacaag
     7801 gaataaagag accaacaata aaataaaacc taactttttc atataaaata cctcattatt
     7861 tttttaatac cagtatagca taatcctgaa tttttgaatc cgtaaaacct tgtct
//
LOCUS       DAZUMO010000104         7866 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000104 DAZUMO010000000
VERSION     DAZUMO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.106"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(658..2004)
                     /gene="gor"
                     /locus_tag="ACG8ER_002611"
     CDS             complement(658..2004)
                     /gene="gor"
                     /locus_tag="ACG8ER_002611"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS5051135.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWKASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2138..3391)
                     /locus_tag="ACG8ER_002612"
     CDS             complement(2138..3391)
                     /locus_tag="ACG8ER_002612"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS5051136.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3397..4320)
                     /locus_tag="ACG8ER_002613"
     CDS             complement(3397..4320)
                     /locus_tag="ACG8ER_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS5051137.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4541..7033)
                     /locus_tag="ACG8ER_002614"
     CDS             complement(4541..7033)
                     /locus_tag="ACG8ER_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS5051138.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7040..7507)
                     /locus_tag="ACG8ER_002615"
     CDS             complement(7040..7507)
                     /locus_tag="ACG8ER_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS5051139.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7643..7716)
                     /locus_tag="ACG8ER_002616"
     tRNA            complement(7643..7716)
                     /locus_tag="ACG8ER_002616"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7680..7682),aa:Arg,seq:acg)
     gene            complement(7774..7847)
                     /locus_tag="ACG8ER_002617"
     tRNA            complement(7774..7847)
                     /locus_tag="ACG8ER_002617"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7811..7813),aa:Pro,seq:tgg)
BASE COUNT         2195 a         1706 c         1265 g         2700 t
ORIGIN      
        1 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
       61 taataccgag aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg
      121 tcctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc
      181 taataccgaa ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca
      241 caattttttt cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag
      301 aactttcttg gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa
      361 tttatttata caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt
      421 ttaaacatcg ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc
      481 ttcgtcagtt acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat
      541 tatcgaagca atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga
      601 ttttctggtt ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta
      661 tcgcatcgtt acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc
      721 ttctttagtt gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc
      781 aacaccaata gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat
      841 ttcgcatttt tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata
      901 aactttcaat tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt
      961 tgcaattggt ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc
     1021 tttctttcca ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt
     1081 cagatctaat ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg
     1141 gtatttatct actttgataa accctttttt gtccaattcc acatttgtat tttccagacc
     1201 taatttttcg gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt
     1261 ttcaccattt tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat
     1321 atgatgtgcg taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag
     1381 cattttatca aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc
     1441 taaaccacaa aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc
     1501 tctcttaggt aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc
     1561 aggaatggtc attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata
     1621 ggtttcaccg tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc
     1681 ctcgacatga ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt
     1741 ttctcgtctt tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta
     1801 acttgaggca tctcgttcaa ttgtttccaa aatcgtactt gctttccaca taactttttt
     1861 aggtacacaa ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac
     1921 ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat
     1981 tacgatgtaa tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct
     2041 ttatttaacg atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg
     2101 cgaagatgct taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat
     2161 ctccaggttt caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc
     2221 ggtcaatgga ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga
     2281 atcctggacc ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg
     2341 tagcaatctg tgttgcctca tcataagata atacttcacc gacaaattta tatactggga
     2401 ttttacgacg ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg
     2461 tagctaattc tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat
     2521 agttttcagg atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag
     2581 atacataatg gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa
     2641 tcaattctgg aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt
     2701 ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc
     2761 catacttcca gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat
     2821 ggttggacaa tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt
     2881 cgatttcggt atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag
     2941 ctaaaacgac acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg
     3001 ctgaagcttg agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga
     3061 tcaaagctgg atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa
     3121 attcccctgc tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct
     3181 tcgcatcatg ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac
     3241 ctgcacggct tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat
     3301 ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca
     3361 attttctttt tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat
     3421 ataaaagaat cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt
     3481 cattttttcc gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc
     3541 gaatagtcga gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca
     3601 tgtacagtaa tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct
     3661 ggcaaaaata tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt
     3721 aggttccgag ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt
     3781 atagtaattc tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag
     3841 ggtttctaca ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc
     3901 acgagcaagt actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct
     3961 cgttactaat gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat
     4021 cgtataaata atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa
     4081 agccaaatat tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat
     4141 attcgctttt ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt
     4201 aaatgaagca ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc
     4261 tgcagcaagc aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat
     4321 aattttctcc tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc
     4381 ttctatttaa agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata
     4441 tattctgtcc aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa
     4501 gtcttactat tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt
     4561 cctttttagg tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt
     4621 ggtcacctaa acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt
     4681 tttgaagtgc gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga
     4741 tcacatcgat tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga
     4801 taatcgcttt gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga
     4861 agacgacagt ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt
     4921 ctaagatacg tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga
     4981 catttttttc ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat
     5041 ttctaaagtc cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca
     5101 gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat
     5161 atggtttttg acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg
     5221 gaggagaccc gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac
     5281 ggatcagggc atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt
     5341 ttccgacacc agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca
     5401 atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa
     5461 cgacacgttc atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca
     5521 actgctgtag agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta
     5581 cacgatcaga ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt
     5641 tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga
     5701 tcgctgcttc cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg
     5761 ttaagtgatc ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg
     5821 ctttatcggg tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta
     5881 cagcttcatc tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa
     5941 gaatcacttc tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct
     6001 ctaatgcaga atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg
     6061 tttgtaattc tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt
     6121 ctgctccacc tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat
     6181 cattatagat ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg
     6241 taccagcaac caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg
     6301 gcacttcacc taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac
     6361 caggttcacc caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc
     6421 gtttcacttc tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg
     6481 tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt
     6541 gtacatttac atttctacga cgattcattc cttttccagc agaatcacca gctagcccca
     6601 ttttttgttt cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc
     6661 gagtagccat gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt
     6721 tttgagaacc agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg
     6781 gtgaatatgg aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat
     6841 gttcaatttc ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag
     6901 caattccatt ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat
     6961 gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa
     7021 aaagttcttc catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc
     7081 ggagaatccg agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa
     7141 ttgcactcaa catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt
     7201 gaataatagc aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct
     7261 caagtaattg ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac
     7321 cacgtttgct ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt
     7381 tgatttgtga aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca
     7441 tttcactatt ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat
     7501 gtgccatctt attcattccc tttgactaat attgacctta attataacga atttgataat
     7561 agaaagaaag aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac
     7621 ggaatatact attccgtatc gacgcgccca agaggagtcg aacccctaac cttttgatcc
     7681 gtagtcaaac actctatcca gttgagctat gggcgcatat tcaatttaaa aaaattataa
     7741 atcatttgaa aacaaaaagc aagataaaaa aaatcgggaa gacaggattc gaacctgcga
     7801 ccccttggtc ccaaaccaag tgctctacca agctgagcta cttcccgttt aaaaaataaa
     7861 aaagac
//
LOCUS       DAZUMO010000105         7488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000105 DAZUMO010000000
VERSION     DAZUMO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.107"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1392)
                     /gene="eno"
                     /locus_tag="ACG8ER_002618"
     CDS             complement(94..1392)
                     /gene="eno"
                     /locus_tag="ACG8ER_002618"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS5051140.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1508..2263)
                     /gene="tpiA"
                     /locus_tag="ACG8ER_002619"
     CDS             complement(1508..2263)
                     /gene="tpiA"
                     /locus_tag="ACG8ER_002619"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS5051141.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2367..3557)
                     /gene="pgk"
                     /locus_tag="ACG8ER_002620"
     CDS             complement(2367..3557)
                     /gene="pgk"
                     /locus_tag="ACG8ER_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS5051142.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3667..4668)
                     /gene="gap"
                     /locus_tag="ACG8ER_002621"
     CDS             complement(3667..4668)
                     /gene="gap"
                     /locus_tag="ACG8ER_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5051143.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4709..5800)
                     /locus_tag="ACG8ER_002622"
     CDS             complement(4709..5800)
                     /locus_tag="ACG8ER_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS5051144.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5949..7283)
                     /gene="rpoN"
                     /locus_tag="ACG8ER_002623"
     CDS             complement(5949..7283)
                     /gene="rpoN"
                     /locus_tag="ACG8ER_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS5051145.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7343..7414
                     /locus_tag="ACG8ER_002624"
     tRNA            7343..7414
                     /locus_tag="ACG8ER_002624"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7376..7378,aa:Arg,seq:ccg)
BASE COUNT         1939 a         1581 c         1385 g         2583 t
ORIGIN      
        1 atatttcagc aagtagagag ttcgattact taactaagtg atcatactcg actatctttc
       61 aatagtcgat catgtatctt cattagaaaa gaattatttg ttttttaagt tgtagaaaga
      121 tttcaaacct ttgtattctg caacttcacc aagttggtct tcgatacgta gtaattggtt
      181 gtatttagca atacggtcag tacgtgatag agaaccagtt ttgatttggc cagcgtttgt
      241 tgcaacagcg atatcagaga ttgttgaatc ttctgtttca ccagaacggt gagatacaac
      301 tgctgtgtag ccagcttctt tagccatttc gatagcttcg aaagtttctg ttaatgtacc
      361 gatttggttc actttgataa ggattgagtt agcaattcct ttttcgatac cttcagataa
      421 tttttgagtg tttgttacga acaagtcgtc accaactaat tgaactttgt cgcctaatac
      481 gtcagttaat ttcttgaatc cgtcccagtc gttttcgtct aatccatctt cgattgagat
      541 gattggatat ttagaaacta attcttcgta gaatttgatc atttcgtcag ttgttttttc
      601 gccttcgcct gagtcagcta gtacgtaaac acctttttct ttgtcgtaga attctgaaga
      661 agcagcatcc atagcaagaa caacgtcttt accaggtaca tagccagctt tttcgattgc
      721 ttcgatgatt acttcaaaac cttcttcgtt tgaaccaagg tttggagcaa atccaccttc
      781 gtcaccaaca gaagttgcta atccgcgagc tttcaagatt gaagctaatg cgtggaatac
      841 ttcagcaccc atacgtagag cttctttgaa tgttggagcg ccaacaggca tgatcatgaa
      901 ttcttggaag tcgattgagt tatcagcatg tgatccgccg ttgatgatgt tcatcattgg
      961 tgttggcaat acttttgtgt tgaatccgcc taggtagtgg tataaaggta cttctaggta
     1021 gtcagcagct gcacgtgcaa cagcgataga aacacctaaa atagcgttcg cacctaattt
     1081 acctttgtta ggagttccgt ctaaagcgat catagctttg tcgatagcca tttggtcacg
     1141 aacatcgtag ccaatgattg cttcagcaat gatgttgttt acgttgtcga cagctttagt
     1201 tacaccttta ccgccataac gagctttgtc tccgtcacgt aattcaactg cttcgtattc
     1261 accagttgaa gcacctgaag gaaccattcc gcggccaaaa gctccgcttt ctgtgtatac
     1321 ttctacttcg attgttgggt taccacgtga gtctaagatc tcgcgagcgt aaacatcagt
     1381 aataattgac atgttttgtc tctcctttga gtttgttttt tcctaaggga tttccctcat
     1441 atacgatttt agttaatttt gctcattagt gcaaacattt cttgcatttt tcgcaaattt
     1501 tcaacaatta ttttacagcg tctaacaatg acaagaatga atctgcttgc aagcttgcgc
     1561 caccgactaa agcaccgtca acattttctt ttgccatgta ttcagcaata ttttctggtt
     1621 ttacagaacc gccgtattgg atacgaactg cttctgatac ttcttttcca taaagttttt
     1681 caactgtttg acgtacaact ccacaaattt cgtcagcgat ttgtgcgtca gctgatttac
     1741 cagtaccgat tgcccagatt ggttcataag caataaccat gtttgacacc tgttcagctg
     1801 aaagatcagc taaaccagct gtaatttgtc cttcgatcca ttcagcagtt ttgcctgctt
     1861 cataggtttc taaagattcg ccgcagcata ggattggtgt cataccattt gcaaagatag
     1921 ctttcgcttt tttattgatg tcttcgtctg tttcatggaa atattcacga cgttctgaat
     1981 gtccgatgat cacataatga acacctagat cagcaagagc tgctggtgaa gtttcaccag
     2041 tgaacgcacc tgagttttcc cagtagcagt tttgtgctga gatttttagt tctgtacctt
     2101 cagctgatgt cattaattct tgtaagaata atgcaggaga tccgatgact gaatcaactt
     2161 tgtcatttga agggattttg ttttttactg cttcagcaaa ttcttttgct tctgatgcag
     2221 ttttgttcat tttccagtta cctgcgataa taggtttacg cattgaggaa cacactcctt
     2281 aatttattga aaaatggcta gggagtcatg aaaactccct tcaccttttg ttcgattact
     2341 cgtcagaatt gagcaaactt ggtcgtttat ttatcgttga ttgctgctag tcctggtaat
     2401 tctttacctt caagcaattc caagcttgcg ccgccacctg ttgagatgtg tgtgaatttg
     2461 tcagcaaatc ctaattgttc tgctgctgcg gctgagtctc cgccaccgat gattgttgtt
     2521 gcgccttcta agttagcgat tgcttcacaa acaccgattg tacctttagc aaagttactc
     2581 atttcaaata cacccatagg tccgttccaa acaactgttt ttgcaccttg taatgtatca
     2641 gcaaataatt ttactgatgc tgggccgatg tctaaagcca tcaaaccatc aggaatgtct
     2701 ccttcgatta cttgtgtttc aacatcattt gaaaattctg gtgcacagac attgtcgatt
     2761 ggcaagacaa gtttgtcgcc tgctttttcg attaattctt ttgctagttc cactttatca
     2821 gcttctacta gagagttacc gatcttgatt ccttttgctt cgtagaatgt gtaagtcatt
     2881 ccgccaccga tcaagatttt atctgcttta ggaatcaagt tttcgattac gccgatttta
     2941 tcagaaactt ttgcgccacc aaggattgca atcattggtc gttttggttc ttctactgct
     3001 tcaccgatga acttgatttc tttttccatc aagaatccag caactgttgg gataccagtt
     3061 gaagcgatac ctacgttaga agcatgtgca cggtgagctg taccgaaagc atcgttaaca
     3121 aatacatcgc ctaatgaagc ccagtattta cctagttctg tatcattttt gctttctttt
     3181 ttaccgtcaa tatcttcaaa tcgtgtattt tcgaatacga caacgtcgcc gtcattcatg
     3241 ttgcggattg cttcttctaa ttgttctccg cgagtttcag gtacgaatgt tacttcttta
     3301 cctaataatt cgcctagacg ttttgcaact ggagctaaag attttccttc tttatcttct
     3361 tctgttttta cacgtccaag gtgagagaaa aggattgctt ttccgccttg ttctagaacg
     3421 tagttgattg ttggcaatgc tgctttgata cgagtgtcgt cagtgatcac gccgtctttc
     3481 aaaggaacgt tgaagtcaac acggacaaga acttttttgt cttttaaatc gatatctttc
     3541 actgttttct tagccatttt atttcctcct gaataaactt attatcaaaa aaagcggaag
     3601 aagcgcgatt gcttccccgc ttttaaatgt acatgtctat aatttacact tgtacgtttg
     3661 tctatcttat aagttagcga agtattctaa agtacgtact aattgtgcag tgtatgacat
     3721 ttcgttgtcg taccaagcaa cagttttaac taattgtttg tcgccaactg tcattacttt
     3781 agtttgtgta gcatcaaata atgaaccgta agtcatacca acgatatctg aagaaacgat
     3841 ttcgtctgtg ttgtatccgt aagattcgtt tgaagcttct ttcattgctg cattgatttc
     3901 gtcaacagtt acttcttttt caagaactgt tactaattct gttaatgaac ctgttggtac
     3961 aggaacacgt tgagcagctc cgtctaattt accgttcaat tcgggaatta ctaaaccgat
     4021 agctttagca gcacctgttg agttaggaac gatgtttgca gcagcagcgc gtgcacggcg
     4081 gaagtcacct ttaggatgag gtccgtctag agtcatttgg tcacctgtgt aagcgtggat
     4141 agttgtcatt aatccttcaa caacaccaaa tttgtcgttc aatgttttag ccataggagc
     4201 taaacagtta gtagtacatg aagcacctga gataactgtt tctttaccag ttaatgtttc
     4261 gtggtttgtg ttataaacga ttgttggaac atcgtttccg ccaggagcag agataacaac
     4321 gcgtttagca ccagctgtta aatgtttttc agctgctgat ttagaagtga agaaaccagt
     4381 acattctaga acgatatcta cgcctagttc gccccatggt aattcttctg ggttgcggtt
     4441 agctaatact ttaacttctt ttccgtttac gttgaatgaa ccttcatgaa cttcaactgt
     4501 tccgttgaaa cgtccttgag ttgtgtcgta ttttaacaag tgtgccaaca ttttagcatc
     4561 tgtcaagtcg ttgattgcta ctacttcgat tccatctaca tcttggatac gacggaatgc
     4621 tagacgtcca atacgtccaa aaccattaat acctacttta actgtcattt aagatttcct
     4681 ccttatgaaa atcaaaaaaa tatttatttt aaagggttac cccttttaaa atctcatttg
     4741 cagcagcttc atcagtgatg agccacgttt gttttggtgc atttttcata taagctcgaa
     4801 ttgctttagc ttttgatttt ccgcccgcga ttgccatgat gatcgggact ttttccaaat
     4861 cttgcaattc tatgccgatt ctagggacct tataaacgac ttcacctttt tcattgaaaa
     4921 agtagccaaa agattccgca acagcatttg cttgtttcag catgactaat tctttttcag
     4981 tcatcttacg acgtgctgcc atgtgtaatg cacgcccaat actatgaata acacaattcg
     5041 cttcactaat caagtttaag acttcttgga tagatggctc attgagtaaa gaattgtaag
     5101 ttgccagact taattgttcc ggaacataaa gtgcgcggtg gttaccgttt gctttagcag
     5161 ccattcttgc actgacagaa tttgcctgta cagttatcgc ttcaccaatc ccgcctcttg
     5221 cagggacaaa caggttatgc cgtttcttat tttccagatt agacaattgt tcggctacca
     5281 cagccatagt agttcctccc atcacagcga tgatattctc gccttctgga agtagtcggt
     5341 ctaaggcttc gctcagcgct tcgccgaatg catcagccac tttggtctgc tcttcgctgt
     5401 ttcctgaaac aacgatacag cgttggttac caaaatattc tgccaactgt tgttcggttt
     5461 gatgcgtacc taaaagctga tccatgaagt tttcaaggct ttgataaact tcttcgccct
     5521 tagttgtcag cgtcataccg ctttttgagg tgtcgatcaa attcagattt ttcaaaaggt
     5581 ctgtttctgt tcgtaagact cgttctgtga tccccatact ttccgataaa cttcgacgcc
     5641 caatcggctg catccaatag atatttcgta aaattttata gcgttcttgc aacacttcga
     5701 taatatctgg tgcaacagat tcaatcattt ttatttcatc cagcatagcg atctccttag
     5761 ttggacactt tacgtccaat gtagacttaa aacgacccat atacgataaa aaaagaaatt
     5821 aactattcga atttcacatc aataaaggga aatctcaacc tcacgactgt aagtctaaca
     5881 gttttgctct cgtttttcaa ctaaaaagac tagaaaaagt gaaatacttt ttctagtctt
     5941 ttgactgatt agttatcata acgttttctt tttgatgaac tcgggattcc taataaatca
     6001 cgatattttg ctactgttcg tcttgagatc gctatctcat cttttttcag caattcaacc
     6061 aatttctggt cagacaacgg cttgtttttg tcttcttgat cgactaattc ctgcagcttt
     6121 ttcttcgctg tatctgctga aaggtcttct gtctgttctg tactattcgt aggaattcgt
     6181 gtagtgaaaa atttttttaa ttcaaataca ccgaagtctg tttccaaata tttcccgttg
     6241 actgcacgac tgaccgtgga ttcatgcaca tctatctcag ttgcaacttc ctttaacgta
     6301 agaggcttga ttggacgttc cttgtcgata aagaaagctt tttgccggga aacaatgact
     6361 tgagcaaccc gcaaaattgt atccccacgt tgtaggatcg ttttcttcaa ccattcgaat
     6421 tcctgtttcc gttcttttaa ataattttca gtttctttat ctgcttgttg tgacatttgt
     6481 ttgaagtaac tctcttgaaa gcgtatattt ggctgatttt ttcggtttga gataacttgt
     6541 acatttttat cgtcgtccac taatacccgg acatcaggaa taatgtacaa ttcgctgcta
     6601 cggtcgaaaa tccggccagg gcttggactc aacgtttgta aatagtcgaa tatctgctga
     6661 acagcatgta gatcaacaga aaaacgttgt gcgatttctt tccattttcg ctccaccagt
     6721 tcctcaaaac attcttccaa cacgatataa gctaattccg gcgcataatc atctcgttct
     6781 gtttgaagca tgagacattc ttgcaaacta cgtgcaccta ctccagctgg ttctaattgc
     6841 tgaatcagag tcaacgcatc gagcatttgg ataggcgtag ctccaatctc ttttccggct
     6901 tcttctaaac taatcgtcaa aaatccgttc aaatcgatgt attctacaag ataaagaacg
     6961 attttcctca aaaaagtatc tcggtaattc aaatggactt gttcgatcaa tgaatcaaat
     7021 aaagaccctt ttgtatcagg aatctgattt aggtaatttg tctcttctcc ttcataagaa
     7081 gatgaagaag cttttatgta atctggctgc cattccggct caacaacttc caccaatgga
     7141 ttttccattg cttgattctc aacgaatgcc aacagctcct cactgttata ttgtaaaact
     7201 tggatggact gttgcaactg ttgcgtcatg gctagtttct gtgtctgctg ctgtttttgt
     7261 gaaaaatttt gctcaaattt catttttttg tccaatcccc ttgtaatttt ttatctgttt
     7321 agttacaatt atcccagtgc ttgcgtcctt agtgtagtgg atatcacgta agattccggt
     7381 tcttgagacg ggggttcgat tccctcagga cgcgttctac tgctatttta gcataattta
     7441 cgaatagttg cttctttatt tttaagtgaa aactgtccgc caaagaaa
//
LOCUS       DAZUMO010000106         7348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000106 DAZUMO010000000
VERSION     DAZUMO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.108"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..681
                     /locus_tag="ACG8ER_002625"
     CDS             <1..681
                     /locus_tag="ACG8ER_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5051146.1"
                     /translation="YNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSW
                     VIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSII
                     YFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLF
                     YNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARR
                     FDSDSALF"
     gene            693..1613
                     /locus_tag="ACG8ER_002626"
     CDS             693..1613
                     /locus_tag="ACG8ER_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5051147.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1654..3111
                     /locus_tag="ACG8ER_002627"
     CDS             1654..3111
                     /locus_tag="ACG8ER_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5051148.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3456..4070
                     /locus_tag="ACG8ER_002628"
     CDS             3456..4070
                     /locus_tag="ACG8ER_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5051149.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4063..5787
                     /locus_tag="ACG8ER_002629"
     CDS             4063..5787
                     /locus_tag="ACG8ER_002629"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5051150.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5784..7235
                     /locus_tag="ACG8ER_002630"
     CDS             5784..7235
                     /locus_tag="ACG8ER_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5051151.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2508 a         1222 c         1468 g         2150 t
ORIGIN      
        1 tataatgtag catttatcct tttgaatctg ctgatctcag tcgtatttgc aatcattatg
       61 agcgagttga gaaataaacg actcgttaaa gtttatcaaa cgatgtcctt actaccttat
      121 tttctatctt gggtcattat cagttacttt gtttacgctt ttttaagtcc tgacaaagga
      181 atattcaatc agtggatcac tggacatgga ggagaagctg tcaattggta taatgaaccg
      241 aaatattggc cgtttatctt ggtgttcatt ggtacatgga aagggattgg ttataacagc
      301 attatttact ttgcatctgt tatgggaatc gatccgactt attatgaagc tgctatggtt
      361 gatggagcta gcaaatggca gcagatcaaa catgtgacga taccacaact tattccacta
      421 atgacgattt taacgatttt agccgtagga aacattttcc gagctgattt tggcctgttt
      481 tataacattc ctaggaattc cggtgcatta tatgatgtga ctcaagtttt ggatacgtat
      541 atctacaatg gcctgacatc cacaggagat tttggtatga cggctgctgc aggactttac
      601 caatcagttg tcggctttgt cttgttgatg atcacaaata cgatcgcccg acgttttgat
      661 agcgattctg cattattcta aggaggaaaa aaatgaaaaa aaagaaagtg gagagagtcc
      721 aagtccgctc tttcaataag acggctaatt ttttctctaa tataatgatt gcactctttg
      781 ctatttcctg tattttgcca tttatatttg ttatcgccat ctcttttacc gatgagtcca
      841 tattgtcgac tcacgggtac agtttttggc cagaaaagtt cagtatattt ggttatacgt
      901 acttgttcga ccagatgcag gataaaattt tccaagcgct tttcgtcact gtacttgtta
      961 ctgttttagg tacactgatc aatagcacgg ctacttcttt atatgcttat gcgatttcac
     1021 ggtcaaactt tccattccgt cgttttttta ccgttttttg tttgattacg atgttgttct
     1081 caccagggat ggtggccaat tatcttgtta tgacaaatct tcttcagtta aaagatacga
     1141 tttgggcatt gatcttgcca atggctgtca gtcccttcaa tattattgtg atgcgcacat
     1201 tctttaaacg ttcggtctct gattcgatta ttgaatcggc acggatcgat ggagctagcg
     1261 aactgcgaat atttgttcag atcgttttac cgttagctgt tccaggtatt gcgacaatca
     1321 gtttatttgc cgcattgggt tactggaatg actggttcaa tgctttacta tacatccaag
     1381 atgataagct agttccacta cagtatcttt tgatgaaaat ccaaagtaat attcaatatc
     1441 tgacacaaaa ttcaggagca ggaagccaat tgacaggcgg attagcctca gttccaggag
     1501 aatctgccag aatggcgatc gtagtcatct cgacgttgcc tattgcgatc agctatccat
     1561 ttttccagaa gcattttgtc aaaggtctaa caatcggcgg agtaaaagaa taaagaaaca
     1621 aaaatataaa tcataaaatt aggaggaaga aaaatgaagt tatggaagaa actagccttt
     1681 acaggtgttt cagcgttgtt gttgggaaca ttagcagcat gcggaggttc tggttctaaa
     1741 gaccaggcag aagctagcaa ttcggacact ttgcagatgt atcaaatcgg agataaacca
     1801 gataatttcg atcaattgat ggaagtagca aacaaacgaa tcgaaaaaga gatcggtgta
     1861 aaagtcaata tcaattacat tggctggggc gattacgaaa agaaaatgaa cgtcatcatt
     1921 tcttccgggg aaaattatga tatcgccttt gcgaacaact atgtttccaa tgcccaaaaa
     1981 ggagcctttg ctgatttgac tgaattagcg cctaagtacg ctgaaaaagc ttataatgat
     2041 ttagatgaag catatattaa aggaaatctg gtcaatggaa aattatatgc tttcccagtc
     2101 aatggaaatg tctttgctca acaagtactt acttttaaca aagctttact agataaatac
     2161 gatctttcaa ttgatggaat ccaatcttac gctgatgcag aaaaagtctt gaaggaattc
     2221 catgaaaaag aaccaaacac ggcagctttt gccattggac aaggcttcaa agtccaaggg
     2281 aactttgatt atcctttagg gaatacgtta ccatttgcta ttgatctgga tggagatgat
     2341 tcgaaaatca tcaaccaata cgataatgaa acgtatatcg gactgttacg tacaatgcat
     2401 agctggtaca aacaaggact cattccaagt gacgctgcaa cgagcaataa ggattatcca
     2461 ctagaaggaa atacttggct aatgcgtgga gaaacacaag gaccgtatga ttatggcgat
     2521 acaattttga caaatgcagc aaaacaagaa cttgtctcaa aagcaattac tgttccacta
     2581 aaatctacag ctcaagcaca aatggcaaat tttgtcgtat caaatacttc aaaaaataaa
     2641 gaaaaatcag tagaacttct agggctgctt aacagtgatc cagaattatt gaatgggctt
     2701 gtttggggga tcgagggaga agcatgggaa aaagtcgatg gttcagatga taaaatcaaa
     2761 ttgctagatg gttatcagcc aaatacacat atgtctgctt ggaatacagg aaacaacaag
     2821 attctatata cacaagagtc tattacggat gacatgattg ccaaacgtga tcaatcgatt
     2881 gctgatgcag aaacttcacc gattttagga ttcagcttca acactgacag tgtcaaaaca
     2941 gaattaagta atatttctaa tgtcatgaat caatatttag atggattgaa tacagggact
     3001 gttgatccag atgaaacatt accgaaattg aaagacgctt tggacaaggc tggctatgat
     3061 aaggtattga aagaaatgca aaaacaatat gatgagttcc gacaagaata ataatgaata
     3121 aagacaaagg ggttgtgaca aaagacgtgt cacaacctct tgttccgaat aaacggtgtt
     3181 caaaagccag tttctcggta ttaagctaaa tatatgtaaa aatgtggaaa gccatattca
     3241 caaaattttt cttactactc gaaactaagc ggctatttca caaactcttg gtaaacggtg
     3301 ttcaatcagc tgaccctcgg tattcgctca caaaacgaca aaatatgagg aactattttt
     3361 ccgtttgttt ctctaataat caggtcaagg tgctgattgc ccaacctttt tttgtataat
     3421 gagaaagtat aaacttaatg tttggaggag aagatatgtt agggaaggtt ttggaagagt
     3481 tatttatccg gatctgggtc attataaaac taaccttgta tttctggata tatacatttg
     3541 ccggcgggat catctttgga ttaggtgcgg catggaaaac agtcaatgaa ctattttatt
     3601 tgtacggatt cgagtataaa gaaatcacaa taaaaagagg ctggaatata tataaaagaa
     3661 attttttaag aggcaatctg ctatttagtt tatttttatc tggtactgct ttattgagtt
     3721 acaatttata tttatctgtt caaatcaaag ggctcatttt tttagctatt gattttattt
     3781 tactttttgc cttgctctgc ctatttgcta cttatcaata cagtatgatt ttagatagtg
     3841 aatatacgat ctctgttcct aatctgctaa agctaagttt tatttcggtt ttttccagct
     3901 tttcgtcttt tctaaaaata attattggta gcggtataat tttaggcgtg acttggcaat
     3961 tcaaaggatt gatcctgttt ggtgtcattg gtcttttgac ggtttggaat ggtacgatga
     4021 caacacattg gcgtgaagaa ttagataaac agttggaaag ttatgaataa gtttcggatt
     4081 ctattttcaa aaggaacctt actaaatcgt ctgttgcgga aatacgcggt tatcatgatc
     4141 ggtgtaacga tgactgctac cgttattttt agtatccata cttgggaaca aaatcaaaaa
     4201 caggcagaaa acatgacaag tgatgccgtt caaagcacaa gcagaatgct gaatgataaa
     4261 acaaccttga gtaggatcat caaaaaccag cttgtaggga attccgaaaa gatcgaaaat
     4321 gtgacgacct acttaacaaa acccatagat cagtatctga tgtatgtata cgaacagcaa
     4381 aacagtaccg atgaattagt ttctttccca aatcaaatca aagatctcta cgcaaattat
     4441 gaagaactaa gtgcgatata tatcgtgttg aatcaactac cggagtatta tgaatcttct
     4501 agagaaaaca aaggtgggga aataaaaaag gggacaccaa aattactaga cgccttttat
     4561 atcaagttgc ctatcacaca aggaggagct gagtcaggaa gtatttttat tggttttcaa
     4621 aagaaagaac tagatatgat tttagaaaat ctaacgtcat tcaatggact ctctttatac
     4681 atgatttctg gaacgacaaa tcgactatac acattccatg ataataaaat agctaaagaa
     4741 acgtttgaaa gacaagaaat gatcatcaca gagagtctga agaaaaattc acaattgccg
     4801 attgacgatc tggaaaaaaa caattttgtt caacaccaag aactgtctga tgattatcgt
     4861 atattagctg tactagataa aaaaagtgta aaaatcgaaa cgggaaaaaa tcttgccccg
     4921 ctgatcattg gtatcattat tttagacaat atactgctta tctttcttta taaaacattc
     4981 aaacgatatt cacagcaagt atctatcgtc atgcaggcca tggatcaaac agcagcaggg
     5041 aatttagaga cgcggatcga cataacaaac acggaatatg aactgaagga actttcaatc
     5101 ggtatcaacg aaatgttgga tagcatcaat cagtttgtag aagacatcta caaattagaa
     5161 atcaaacagc aagacgcaca tatgcgggcg cttcaagcac agatcaatcc acacttttta
     5221 tataatacat tagaatatat ccgaatgtat gcaatcagcg aaggaagtga agaattagca
     5281 gatgttgtgt atgcgttttc cgctttgctt cgaaacaata cgaatcaaga gaaaacgatc
     5341 actttaaaag aagaactgga cttttgcgaa aaatatgtct atttgtatca aatgcgttat
     5401 cctaatcgag tagcctatca ttttatgatc gatcctgatt tggaaaagat cgaagtacct
     5461 aaatttgtga tccagccatt agttgaaaat tattttaaac acggtattga ttttactcga
     5521 ttcgataatg cgctaagtgt caaagtcttg caggaaggca aacgggtaag gatcatcatc
     5581 aaagataatg gaaaagggat gactgaaaaa cggctaaaac agattgaaga aaaactgtct
     5641 catcctaaag tggaacttca cggttctatc ggtctgcaaa acgtgaatga acgtttacgt
     5701 gccagcttcg gcagcagtta ttatatgtcg cttgagaata atgagacagg tggactgacc
     5761 gtttctatta cattcaagga gggatgagcg tgtatcaagt gttattggta gatgatgaat
     5821 acatgatttt gaatgggtta aaaaagataa ttgattggca aagtttaggc tttcaaatag
     5881 tagcaacagc agagaatgcc aaagaaggac tagctgtact tgaacagcga cagattgatt
     5941 tagtagtaac ggatgtgaca atgccagaaa taaatggact ggaatttatt gaagcggctc
     6001 aaaaagaacg tcataatttt gaatttatga ttttatcagg ataccaagaa ttcgattatt
     6061 tgaaaggagg tatgcaatta ggcgccgtga attatttgat gaaacccgtg aataagttcg
     6121 aattggttga aagcctcaag aaaatcaaaa cacggttaga tcagcaaaat gaacaaaaaa
     6181 atcaacaaga aatctatcaa gaactgttat tcagtcaatg gttgaatgaa gaattggatg
     6241 aaataagtga agaagaaata ataggacaaa taagtgagaa gcagcatagg atagtattga
     6301 ttcaactggc aagaactcat gatactctta tcaatagatg gttgcaagaa cggcaacaaa
     6361 tcttttacta tcaacgaaat tacggagacc gtgtattgat cacgttgttg ttggaggcag
     6421 atgaagttga tggcttctgt tgttttgttc aagaaaactt tcctgatcag gaatggctga
     6481 tcagtatcgg ggaagagaca gattctttgg acaaaatccc tgaaacattc caacaagcga
     6541 aagacaatct acagttgcat caattttatg gagataaaga cgagcatgtt ttttacgcag
     6601 tacaagcaga aacaaaagat caaaccatcg atttttcaag tttcagacgc gtgctgcaaa
     6661 ataaacaatt ggaagtcgct caaaaaatga tcactgattt ttttgaacag tttcagttag
     6721 cagccatgat gccggaagat atacgatatt ccagtttttt actgtttatg gagattcaac
     6781 gggaattgat tgatctggaa gatgaggaat atttacaagg aatcgaaaaa atccatcagg
     6841 caaaaacggt gcaagaactt cagcagctac ttctgtcatt catcaaaaag catcagagac
     6901 agaaaaagta cagtgacaat gtggaaaaag taatcgtaat cctgcaccag cattatcaag
     6961 aaccacttac actcaaagaa gtgtcagaaa acctgcattt aaatgtcatg tatctaggac
     7021 agctgttcaa aaaagaaacg aaaaaaagtt tttcagctta tttgaaccat ttacgtatgg
     7081 aaaaagcgaa acaactgctt ttgcacagta atcaaaacat caatgagata gcaagtgaga
     7141 ttggctacaa caacaccacg tatttctcta aattattcaa gaaaatcgtc ggcagatccc
     7201 ctaaagagta ccgagaaaac agtggagaat cataaaaaaa aaagccaatg taatgaaata
     7261 acttataaaa aattgtctag aaatctaatt ttgggctgtt cttattactt gaggcaaggc
     7321 agctttttca tgacctctcg ataaacgg
//
LOCUS       DAZUMO010000107         7169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000107 DAZUMO010000000
VERSION     DAZUMO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.109"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..515)
                     /locus_tag="ACG8ER_002631"
     CDS             complement(63..515)
                     /locus_tag="ACG8ER_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS5051152.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            665..1615
                     /locus_tag="ACG8ER_002632"
     CDS             665..1615
                     /locus_tag="ACG8ER_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5051153.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            1608..2567
                     /locus_tag="ACG8ER_002633"
     CDS             1608..2567
                     /locus_tag="ACG8ER_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5051154.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            2564..3319
                     /locus_tag="ACG8ER_002634"
     CDS             2564..3319
                     /locus_tag="ACG8ER_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5051155.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            3342..4295
                     /locus_tag="ACG8ER_002635"
     CDS             3342..4295
                     /locus_tag="ACG8ER_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5051156.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(4939..5235)
                     /locus_tag="ACG8ER_002636"
     CDS             complement(4939..5235)
                     /locus_tag="ACG8ER_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS5051157.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            5590..5946
                     /locus_tag="ACG8ER_002637"
     CDS             5590..5946
                     /locus_tag="ACG8ER_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5051158.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            5956..6855
                     /locus_tag="ACG8ER_002638"
     CDS             5956..6855
                     /locus_tag="ACG8ER_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5051159.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         2202 a         1214 c         1434 g         2319 t
ORIGIN      
        1 tatttgatgt caatctgatc aaaagctttt tttgcatgta aaaatttatg tgttttttcg
       61 ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt ttcagctggc
      121 gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt tactgcttct
      181 tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc gttgactaca
      241 gtgaaacggc tattgttttt cgttgctagc tggatatatt ggttttggac agaaaggggt
      301 agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt taaaaagttt
      361 tctttgtttt ttggttggat catttcagca acagtcatac taagatagca gcgttccctg
      421 aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc ataaagtccc
      481 ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct tcttctcttt
      541 gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa aaagcggttg
      601 cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta gaaaggtgga
      661 ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta tttcattgtt
      721 catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc aaagcttaca
      781 aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg gagcgaccag
      841 tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt caccaacgac
      901 tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt tcctacctgg
      961 tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag gaacattgct
     1021 ttttttatcc cttacgcgat tgttcctcca gaaaaatcaa ttgatgcttc cgttgattgg
     1081 agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc aattccagtt
     1141 ggtacaaaat atgacttcat ggcttcaagg gaattttgcg actgtgatac gaggaaatta
     1201 tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg cttaccgatt
     1261 tacgatcatt ggtctgggcg aatcgtttgc agtaaatctg ggaatagatt accagaagat
     1321 gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga tcatggtagg
     1381 aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat atggagatca
     1441 agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag tattttgcga
     1501 tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga tcgttggaat
     1561 catcggtggg gctatcttcc tttatcttct gataagggga cgacgatatg cttaaaaaaa
     1621 agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga atgtacttga
     1681 cgatccatac atatggaaac tggcattttg catgtgcatt gaggagtaag aaaatttttg
     1741 catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag acaattacgc
     1801 ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc cttgtccaaa
     1861 cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt acagtttttt
     1921 tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc tttcttttga
     1981 aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta ggtacgcttt
     2041 ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat gatttgcttc
     2101 aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg cctgcttctt
     2161 tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta gacgttttcc
     2221 atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt gaatttttga
     2281 ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt ccagttactt
     2341 ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat cgtcatggtc
     2401 agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga cagctcgcag
     2461 tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt gtcggaggaa
     2521 tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg atttacaaaa
     2581 ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa cgattcctga
     2641 aaagaagttg actgctttta tcggtccaaa tggtgctgga aagtcaacgg tgttgtcgat
     2701 gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata acgaagtaaa
     2761 ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat cgaatgaaat
     2821 aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt atacgaaagg
     2881 aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacaca ttgggttaga
     2941 agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac gtgcgtatat
     3001 tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt tgaacaatct
     3061 agatatgaat tttgcggttc aaatcatgca gattttaaaa aatttagtac aagaaatcgg
     3121 gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg cagacgaaat
     3181 tgttgcaatg aaagatggac agttatttac gcaaggaacg acccaagaaa tgataaaaaa
     3241 agatgtattg gatgaattat acgaaatgga catacggatt tgtgagattg aaggccgcag
     3301 attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa ttagtagtag
     3361 gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagcaac aacaccagtc
     3421 aggcagacac aaaaaaaagc agcacacaaa ctgaaactac attgacgata acagatagta
     3481 atggagacca aatcgaggtg ccaaataatc cagaaaaagt cgtcgtattc gacaatggtt
     3541 ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca gctacaagca
     3601 gccttcctga gtatctttca tcctataaaa aagtagaatc agcaggtgga ataaaagaac
     3661 cagacttaga aaaaattaat cagcttcagc cagatctaat catcatttct ggccgacaaa
     3721 gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct ttagatgcga
     3781 aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc tttggtaagc
     3841 aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag acgaaaaaga
     3901 aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg caattatcag
     3961 cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt gaacaagcag
     4021 atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat gtgctggaaa
     4081 aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga gacgacagca
     4141 aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa aatcagcaga
     4201 tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa tcaatgaaac
     4261 tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg attaaactct
     4321 catactatgt aacaataatg tttcttacat tcttaaatag caagagccta tcctcaaaga
     4381 agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt tttatttatt
     4441 tgagaaatct tttttcaacg tgaattcatt atctacatca tacgggttga ttctagaatg
     4501 gggagtggca aaaaatagtg tgatccattt tttcagcatc aagatcgcct cctttgattt
     4561 gaatcaaagg tacaaagcga aacttagata aaacttaaat ttagatattt ttattttgta
     4621 ccgatctctt ctttaaacga tgttccatct gctgcttctc ggcaataagt ccactagact
     4681 agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaaaca aatcgcagtt
     4741 gtcacaatct cttctaaacg atgttccatc tgctgcttct cggcaataag cccactagac
     4801 tagaaaatat gagaagctat tttctgtcca gcgttcttat tactcaaagc aaatcgcagt
     4861 tgtcacaatc tcttctaaac aatgttcaat cagctgacct tcggtattag ctcaaagcac
     4921 tgatttcaca acctctattt aggcatgtgt ctcagcaagc ggttgatagt tgatgatctg
     4981 catttttcga taaaaatcgg cgggaagctt actccaccgt tttagtaatt gatagaaata
     5041 actctcttct gctttgattt tatactcgct taatatcaat ccaaaactat tcgttttttt
     5101 tgaagctaat tttttataaa tatccaatgc tttggtttta tcttcattga caaagagtgt
     5161 ttttacttca atataagaac cctcgatatt ttgtgtttct tcgataaaat agatgttttt
     5221 tatatattcc ttcatagtga ttcctccaaa gaagtctttt atttgataag tttactctac
     5281 cgaagagttg ttaataatcc gtgaacaaaa agtaattctt tttccaatag ttgttaaagg
     5341 ttttttattt tttatagtat tttttgttat ttaagaaagg aataaggtta aacaaataac
     5401 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
     5461 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
     5521 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
     5581 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
     5641 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
     5701 caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa agatacacaa
     5761 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
     5821 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
     5881 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
     5941 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaat
     6001 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
     6061 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
     6121 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
     6181 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
     6241 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
     6301 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
     6361 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     6421 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     6481 cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa caaattaaat
     6541 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     6601 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     6661 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     6721 gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg tgcccatcac
     6781 ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca agcgatgaca
     6841 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     6901 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gcggaaaata
     6961 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     7021 gtattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt ttatttaatt
     7081 ataattaata ttgattgaag tctaggcagt caagtcgaga gtgtaaaata ttttgtgtaa
     7141 atgaaaaaat ccatacaaaa aaggaagtc
//
LOCUS       DAZUMO010000108         6989 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000108 DAZUMO010000000
VERSION     DAZUMO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.110"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8ER_002639"
     CDS             127..579
                     /locus_tag="ACG8ER_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051160.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8ER_002640"
     CDS             593..1282
                     /locus_tag="ACG8ER_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051161.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8ER_002641"
     CDS             1407..3758
                     /locus_tag="ACG8ER_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS5051162.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG8ER_002642"
     CDS             3838..4233
                     /locus_tag="ACG8ER_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051163.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG8ER_002643"
     CDS             4558..5175
                     /locus_tag="ACG8ER_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5051164.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG8ER_002644"
     CDS             5216..5515
                     /locus_tag="ACG8ER_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS5051165.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG8ER_002645"
     CDS             5549..5716
                     /locus_tag="ACG8ER_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5051166.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKKYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6231)
                     /locus_tag="ACG8ER_002646"
     CDS             complement(5761..6231)
                     /locus_tag="ACG8ER_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5051167.1"
                     /translation="MEFREGAFDFQTSQQNPSLDRLGRDYEDIKNTVAFMKQKKVALK
                     ILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKG
                     RPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6237..6917)
                     /locus_tag="ACG8ER_002647"
     CDS             complement(6237..6917)
                     /locus_tag="ACG8ER_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFS5051168.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
BASE COUNT         2354 a         1016 c         1374 g         2245 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaaatactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgggttctgt tgcgaagttt gaaaatcaaa cgcgccctct ctgaactcca aatatcttag
     6241 gctgttattc ccattaatac cttgatttca gtagacaccg aaaagccgaa gagcgttcca
     6301 tttcttcggt tctttttata tattcctcga atggtctcca tgcccttaat cgtggaagag
     6361 gctgtacgga gactttgata aaatttattt cgtcgtttaa taggtcgatg gtcttgttct
     6421 attaaattgt taagatactt cacagttcgg tgctctgtct tagtatataa acccacactc
     6481 tgtaactttc taaaggcgga gccaagagaa ggtgctttat cggtcacaat tgctttcggc
     6541 tcaccaaact gtttatggag tcgttttaag aaagcatagg ctgcttgcgt atcccgtttc
     6601 tttcgtaacc agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc
     6661 caacgtccct taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta
     6721 tttttcttct tccaaagata atagaggact ttgctgtact cttgcaccca acgataaatc
     6781 gtagtatgac aaacatttat tccacgatca tataacaatt cctgaacttc acgatagctt
     6841 agattgtaac gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg
     6901 cctttaaaat gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat
     6961 agattttttg gaaaactttg caacagaac
//
LOCUS       DAZUMO010000109         6614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000109 DAZUMO010000000
VERSION     DAZUMO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.112"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8ER_002648"
     CDS             complement(105..629)
                     /locus_tag="ACG8ER_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS5051169.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8ER_002649"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8ER_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS5051170.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8ER_002650"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8ER_002650"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS5051171.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8ER_002651"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8ER_002651"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5051172.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8ER_002652"
     CDS             3157..4026
                     /locus_tag="ACG8ER_002652"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5051173.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8ER_002653"
     CDS             4052..4567
                     /locus_tag="ACG8ER_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS5051174.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8ER_002654"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8ER_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5051175.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8ER_002655"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8ER_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051176.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8ER_002656"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8ER_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051177.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8ER_002657"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8ER_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5051178.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1860 a         1395 c         1172 g         2187 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aaga
//
LOCUS       DAZUMO010000110         6614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000110 DAZUMO010000000
VERSION     DAZUMO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.113"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..313
                     /locus_tag="ACG8ER_002658"
     CDS             164..313
                     /locus_tag="ACG8ER_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051179.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            394..990
                     /locus_tag="ACG8ER_002659"
     CDS             394..990
                     /locus_tag="ACG8ER_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5051180.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1003..1902
                     /locus_tag="ACG8ER_002660"
     CDS             1003..1902
                     /locus_tag="ACG8ER_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5051181.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            1905..2389
                     /gene="ssb"
                     /locus_tag="ACG8ER_002661"
                     /pseudo
     CDS             1905..2389
                     /gene="ssb"
                     /locus_tag="ACG8ER_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2411..2668
                     /locus_tag="ACG8ER_002662"
     CDS             2411..2668
                     /locus_tag="ACG8ER_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051182.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2827..2961)
                     /locus_tag="ACG8ER_002663"
     CDS             complement(2827..2961)
                     /locus_tag="ACG8ER_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS5051183.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            3104..3364
                     /locus_tag="ACG8ER_002664"
     CDS             3104..3364
                     /locus_tag="ACG8ER_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051184.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3813..4019
                     /locus_tag="ACG8ER_002665"
     CDS             3813..4019
                     /locus_tag="ACG8ER_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS5051185.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            4019..4270
                     /locus_tag="ACG8ER_002666"
     CDS             4019..4270
                     /locus_tag="ACG8ER_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051186.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            4286..4723
                     /locus_tag="ACG8ER_002667"
     CDS             4286..4723
                     /locus_tag="ACG8ER_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051187.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(4834..5061)
                     /locus_tag="ACG8ER_002668"
     CDS             complement(4834..5061)
                     /locus_tag="ACG8ER_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051188.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIIFFKWITF
                     FLLSYRALPVYFAPPKRRGRLVRNKNHSLSY"
     gene            5159..5347
                     /locus_tag="ACG8ER_002669"
     CDS             5159..5347
                     /locus_tag="ACG8ER_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051189.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IACIGQYLYCIRGRKLYA"
     gene            5465..5671
                     /locus_tag="ACG8ER_002670"
     CDS             5465..5671
                     /locus_tag="ACG8ER_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051190.1"
                     /translation="MKYNLLDHKRMWVEKLEEDLEQLELLGHNKTSTVYKEGLKRTKK
                     AQKELATLESRVQKKEVDLTHTYL"
     gene            5681..5914
                     /locus_tag="ACG8ER_002671"
     CDS             5681..5914
                     /locus_tag="ACG8ER_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFS5051191.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            6236..6439
                     /locus_tag="ACG8ER_002672"
     CDS             6236..6439
                     /locus_tag="ACG8ER_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS5051192.1"
                     /translation="MILGIVLLSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSE
                     IVDRGVKAYVNGFTKVLGGVGGR"
     gene            6429..>6614
                     /locus_tag="ACG8ER_002673"
     CDS             6429..>6614
                     /locus_tag="ACG8ER_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051193.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEER"
BASE COUNT         2396 a         1044 c         1345 g         1829 t
ORIGIN      
        1 tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt tcatttgaag caacgattgt
       61 tttaaaagaa gacttcacta caggctttga attttctgaa aagaaaaaaa cgaacttcaa
      121 gaaaagaaca cgccgcactg caaaataatg gagggttaat cttatgcgta ataccacaca
      181 caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg
      241 tgccagcaaa cgaagactgc agcaattgaa gaaccagcag aggattcaaa aagaaatcga
      301 attagatcga taaaatcttc ctaactattg tatacgtata aacttatgtt atactgtttt
      361 tataagttta tacgtataca aaggagggag aaaatgacaa aagcaatggt atacaagctg
      421 ctagaagaat ttaatggaac tttggctttg aaggatgcca aaaaagcggg catttctcct
      481 gtaacgatca aacgtatggt agatcgaggg gagctggatc gagagtatcc aggttttttc
      541 accttacctg gtcaatttcc tgatgaattg tttatggcgc aaaaaaaata tgaacgcggc
      601 attatttcac atattaccgc gttagatttg tatgatttaa cagatatgat ccctagacag
      661 atcgatctga ctgtacctta cggttatcac gtttctgaaa aaggattgaa ggaattcgca
      721 gttgagctac actatacaaa agcggaatgg tatgaattag gtaagattga aataaaaagt
      781 cgctatggga atcctattat agcttatgat ccagaaagaa ctttatgtga catttggaat
      841 ccctggtata ccgttgagga tgaaatcaaa gtaaaagcaa tcaaaaacta tatggagtcg
      901 gacagaaaaa atcttagaaa attgaatgag tatcgacgaa ttttaccaac agacaaaacg
      961 atgcgttctt atattatggc tttaaattaa aggagaagat acatgacacc tacgcagtta
     1021 aaagcaaaag tgaagaatat ttctagagaa aaagaagttg atccgcagct tgttttaaga
     1081 cattttatga tggaaaaatt cttggaaaaa atctcagagt ctccttatcg tgaaaatttt
     1141 gtgttaaaag gcggcttctt gattggctca aagtatggca ttgaaaatcg cacaaccaaa
     1201 gatattgata cgactttacg ggaaatgaaa gtaaccaaag aaacattaac aacggttcta
     1261 aacgaaattt tttcaacgcc gacgaaagaa ggcattcaat ttgagattca aggaatgaaa
     1321 gaaacgagag aagccgatta ctatccagga tttagcttac gtgtgctggc acatttggaa
     1381 aacatgcggc cagattttaa ggtagatgtg actacaggtg actcgatcta tcctgcaacg
     1441 atcactcaca gtcataaatt gatgtttgag gatcgaacga ttcctttgga atcctatcct
     1501 acagaacaaa ttattgctga aaaattaagt gcaacctttg attttttaac agataattcg
     1561 agaggaaaag atttttatga tctctatact attcctaaaa tggagaagat cgatgagaag
     1621 accttgaaag atagtgtgag aaatacattt acacgtcgag gaaaaactga tccgttaaag
     1681 atatactatg aaaaggatat ggatgtactg aaaaatagtc acgagctaaa agatacctgg
     1741 aagaaatatc aaaaaagcaa tccttatgct gcatcgatca cgtacgatga gacaatggat
     1801 tcgattagta atttgatgga aaaagtgatt cacgtcgaag aaaaagagca aaaactagct
     1861 tatcaaagac accaacagat gaatagagaa atggagagat aaagatgatt aataatgtga
     1921 cattagtagg acgcttaacc aaagatcctg atttacgtta taccgcaagt ggtactgcag
     1981 tagcgacttt taccctagcc gtcaaccgaa atttcacgaa tcaaaacggc aatcgagaag
     2041 ccgattttat taactgcgtg atttggcgca aaccagcaga aacaatggcg acattagcta
     2101 aaaaaggtag cttgattggt gtagttgggc ggattcaaac gcggacttac gacaaccagc
     2161 aaggacaacg tgtatatgtg actgaagttg tagcagataa ctttcaacta ttagaaaata
     2221 aagcagcgac agagagccgt gcgcacgctg atcaatcgtc caccagtcca agcaccgcca
     2281 cctttgaaca acggatacag cgacgcctaa caacaatgga ttgaacgctt cacagaatcc
     2341 atttggtgga caatcaatcg atattagcga tgatgatcta ccgttttagt ttgagaaaga
     2401 aggagtaagc atggcaaaaa aaattagtct gacgatggat ctattgatac ccgtaattgc
     2461 atttatagac gggatttctt tgggcgtcac tgaaaatcct ttggtaaata gaattgcgaa
     2521 aggatcacta tttcttgtat tcgtgtatct tgtctgttct tatcttttaa aaagagcagg
     2581 aaaggataat tccttttggc gattgactaa agtcacagta gcagcgacag ctgttatact
     2641 tacggctatt ctaccgcttt atatgtagag ttaacgggaa atcgttgaac ttcttgcgaa
     2701 atattgctat aatgaacatg aaaaagagga gttgcgctaa caacccctca ttaacagcac
     2761 cgattaaaga acggtggcag aatattagtt atttggaaat aaccgctgac ctgctaaagt
     2821 agacggttat ttctttttgt catttttaag catatttaca atcaaactaa tcaaagcaat
     2881 ggtaaacata ccaaaaccaa gaatggtttg aattgtctca tatgcggaca aaaggcttac
     2941 tccttttata gattttagca cttacattca taagcaccac cacctttcca gaagtcgcca
     3001 ccgtcttcaa actttactgt cttgactatt atagcatatc taaaatcgaa taaaaattct
     3061 caattttaac tgtttttgct ataattttag cgaggtgatc gtgatgaaca aatatgttgg
     3121 tttgcttgat aaaatccgag taataaaaac acaaccatta ttggttcgct ttactttaca
     3181 aacaattcat gaatcgatta attgtgtagt tgcagatatt gaaattatcg ataaattgtt
     3241 aattatggat gatggaaaat acaatattgc cgtcacaggt catttcaata aacggaatca
     3301 attagtcatt gaatcgatgc aaattagaaa ccctgatcat tttacgcgat caatgggcat
     3361 ataaataaaa gaagagctgc aatcctaatg taggactgca gctctttttg ttttacatag
     3421 aaacaggaag aaagggaaag gcctgtttag gaaggcacaa gtgccttctc aattgaaaaa
     3481 cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa cgttgcacgt
     3541 tcactaagat aaggggtctt gctttgtaag acaaaatagg aaaagcttcc tctcaccaaa
     3601 aggttcgttc ctgttcgcta tctctttatt gatttgccag accggcaaca agttgccttt
     3661 tatcaccaaa aagcgacaag gacaagggta aatggcacgc tgcgcgccct tgtccttgtc
     3721 gcttttcgtt gattcattct ggccaacaaa tcaataaaga aagaactaac gaaaggaagt
     3781 aaaacctttc ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat tatttgggtg
     3841 ttcccttcag cgaattattc ccaaactatc atgcaccaca tactgtcgaa tgcgattgtg
     3901 gagaaagagc gaaatgtatc aaaagctatt gtttgtatca atgtccaaca tgcgggaaaa
     3961 aatatacctt gtcttatggc gattatattt tgattgagga gaaaggaaag aaacgctgat
     4021 gaaaacacaa cacaaattat tgactgtaaa agaagtcgtt caaattttgg tggacaaaga
     4081 agtcatttct gaaaaagtag cagaagcttt acttattccg cggtactgga cgatagaaac
     4141 ccctttagta gaggatgcac cgactatgct atttgaagac ctagtgaaga ttgcaggatg
     4201 tgaactgcat tatcaagaaa acttggaaac ttatgaaaac gaatattggt taaccaaatt
     4261 agagaactaa aaaggagaaa aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt
     4321 acctttggat aaaatggcac aaaaaatgga ggatatgacg ttcctataca acgaaacacg
     4381 tgttccaaaa aaacattata aagaaaaatt gtcggtagct gtggaagaaa tgattgaatc
     4441 aggtgtcgaa atgaatctta ttgcgacgta ctatcgaaca ctggaagaac tgaaaaagca
     4501 aaatgcaaaa tggtttttcc aagccttact atgctttgaa ataggtgtca aaccaagcac
     4561 tattaaacct tctgaatacc aggctttaga actaacgtat gcgaaattta tcgaaacgaa
     4621 aaaagccaag actgtttcaa gtgaatggtt agattatttt gaaaatatca ataagtatgg
     4681 cgcatattac accatgaaaa aggaagacaa cgaaaatgaa taaggtgatt tatcaactaa
     4741 caacggattc catatcaacg atttgaaaga aaaaaaggtc aataatcatt tataccgaaa
     4801 agtgagaatg tgtggaataa ggacaaaaaa agcctagtaa ctaagggaat ggttcttatt
     4861 tcgcaccaac cgcccccgcc gcttaggtgg tgcgaaataa acgggcaaag cccgataact
     4921 taagagaaag aaggtgatcc atttgaaaaa gattattccc ttattgttag tactgtttgt
     4981 tggcgaaata cttatgcgag atagtgcagc tgtacagtta aacctcgctg tatttttggt
     5041 aatactggtg ttttgtttga tcttttggcg gtttgtatta gtcctcgtga gtctactatt
     5101 caagatgatt gcagccatat ttttcatggg gttatttcga tgaaaggaga gagtaccaat
     5161 gccaattgca ataggaaata aacggttacc cgtgacatta gatgaaaaga gacaaaaaga
     5221 attgcagcaa ctaaagcaga agtacggcaa aagtgaatcc aggattatgt gtattgcgtt
     5281 agatttattg attgcctgta ttggacaata tctctattgt attcgaggaa gaaagcttta
     5341 cgcttgaatg cgataagtta acccgatata tcgatccaaa attctatgag gataaaaacc
     5401 tggcatggga actggcttta tagacagagt gctagtgcaa gtaaaggaag aaaggaagat
     5461 caccatgaaa tacaatttat tagaccataa gcgaatgtgg gtagagaaat tagaggaaga
     5521 cttagaacag ttggaattgt taggtcataa taagacttca acggtgtata aagaaggact
     5581 aaaacgaacg aaaaaagcac aaaaagaact agcaacactg gaaagtaggg ttcagaaaaa
     5641 agaagtagac ttaacacaca cttaccttta ggagtagaaa atggcagaca agagaaaaga
     5701 aaagattcaa aaattactgg cgcttgcggc ggatgtaaat gatgaggaat cagtgagcgc
     5761 attagccaaa gcccagcaat tgatgatgga ctatgatctt actgaagatg agattttcaa
     5821 ttatcatgag cagcaacgga tagaagcagt agaaacgcac gtgatctatc gtggacgtcc
     5881 gcaaaaatgg ctgtatcgtt tatttagaaa ttaagataaa aaaggctagt taacaggttt
     5941 gttgactggc cttttttgat atgtttgtac atgttttatt gtgttttaat ttttctcttt
     6001 tttatagcaa gctcctttta taataaatat tccgaaaagt gaagaccatt ttttatttat
     6061 taagactatt attattagtg agataaaatt tgattgatct tacagaatat tggtgacgat
     6121 tatgacacta aagatactat ttatgaaaat tttgcaaaaa tgttctgaaa attacatata
     6181 attgttttat aaaaaaggga ggcaattata tgaagaaaaa atttgttagt attttttgat
     6241 tttaggaatt gttttattga gtgtatctac tttaggaatt acagtagatg ctgcaactta
     6301 ttatggaaat ggtgtttatt gtaatactca aaaatgttgg gtagattgga gtagagctcg
     6361 ctctgagatt gttgatagag gagtaaaagc ttacgttaat ggttttacaa aagtgctagg
     6421 tggagtcggt ggaagataaa aaagaaaaac tttttgactt aattgcgaat gcatataatt
     6481 caaagtcata taataatgta gaagtaaaag aaggatttct tcaagatgct aagaaaatac
     6541 gagaggataa accatatcct gaaattatta tatcagtgta caaacattta tctaaattaa
     6601 aagctgagga acgc
//
LOCUS       DAZUMO010000111         6543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000111 DAZUMO010000000
VERSION     DAZUMO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.114"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACG8ER_002674"
     CDS             complement(1273..1938)
                     /locus_tag="ACG8ER_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051194.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACG8ER_002675"
     CDS             2064..4100
                     /locus_tag="ACG8ER_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS5051195.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACG8ER_002676"
     CDS             4104..4742
                     /locus_tag="ACG8ER_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5051196.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACG8ER_002677"
     CDS             4770..5198
                     /locus_tag="ACG8ER_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051197.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..>6543
                     /locus_tag="ACG8ER_002678"
     CDS             5655..>6543
                     /locus_tag="ACG8ER_002678"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5051198.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSD"
BASE COUNT         2241 a          994 c         1211 g         2097 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 att
//
LOCUS       DAZUMO010000112         6535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000112 DAZUMO010000000
VERSION     DAZUMO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.115"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..943
                     /locus_tag="ACG8ER_002679"
     CDS             89..943
                     /locus_tag="ACG8ER_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS5051199.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            944..2281
                     /gene="xseA"
                     /locus_tag="ACG8ER_002680"
     CDS             944..2281
                     /gene="xseA"
                     /locus_tag="ACG8ER_002680"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS5051200.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2286..2510
                     /locus_tag="ACG8ER_002681"
     CDS             2286..2510
                     /locus_tag="ACG8ER_002681"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS5051201.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2512..3402
                     /locus_tag="ACG8ER_002682"
     CDS             2512..3402
                     /locus_tag="ACG8ER_002682"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5051202.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3411..4229
                     /locus_tag="ACG8ER_002683"
     CDS             3411..4229
                     /locus_tag="ACG8ER_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS5051203.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4336..4785
                     /locus_tag="ACG8ER_002684"
     CDS             4336..4785
                     /locus_tag="ACG8ER_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5051204.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4803..6485
                     /gene="recN"
                     /locus_tag="ACG8ER_002685"
     CDS             4803..6485
                     /gene="recN"
                     /locus_tag="ACG8ER_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS5051205.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
BASE COUNT         2208 a         1102 c         1506 g         1719 t
ORIGIN      
        1 ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg gtagaatgaa
       61 aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa aagaattagc
      121 tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata atggtatcca
      181 tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct atgtaagaaa
      241 taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt atcctgagac
      301 gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc ctagttttca
      361 tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga tcctgttagc
      421 tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca gactccttgc
      481 gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt ttgaagctta
      541 ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata tcgtcggtaa
      601 accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc attctaaaac
      661 cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa taggacgtgg
      721 acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg tagggatgaa
      781 tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg agcctattgc
      841 aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga tgctgatgta
      901 tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac aagaatattt
      961 aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc cttatttaga
     1021 acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg cccatcaata
     1081 tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag gagcattcca
     1141 aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc gtatttcatt
     1201 atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg atggaatagg
     1261 tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag gcctttttca
     1321 agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta ccagtccaag
     1381 cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa ttgtccaatt
     1441 ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg tcaggaatat
     1501 ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg ggggtggttc
     1561 gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt atcaagctaa
     1621 tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg atttagtagc
     1681 agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg ttttgtcaga
     1741 agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg tacgccagat
     1801 ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc ggcagcctga
     1861 aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat tatatcaagc
     1921 tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc accaattgga
     1981 acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt atctagaaaa
     2041 acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt ttgctcaagc
     2101 tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct acagctatac
     2161 gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg atttgctgac
     2221 gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca aaggagaata
     2281 aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa attgtcatgc
     2341 agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa cgaggaatgg
     2401 aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg acgaaaatga
     2461 tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga gatgagatta
     2521 actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc ttttttagat
     2581 gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa agcaggcggg
     2641 aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga gcctattgat
     2701 gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc cttgatccac
     2761 gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac caatcataaa
     2821 gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg agcattccag
     2881 ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca gcaattagct
     2941 gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga gggcgaatcg
     3001 aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg gcatttgatt
     3061 cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat cttattgcta
     3121 cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga tctactcgat
     3181 gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg tatggaaaaa
     3241 aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga gattgagctg
     3301 ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt tgatggcggg
     3361 ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga atggaaaaag
     3421 aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa caagccaaac
     3481 gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat aaaccgggag
     3541 aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct tatgtttctc
     3601 gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt gaaggaaaaa
     3661 cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg caaaatggcg
     3721 caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa ataagacaag
     3781 atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc gaagacttta
     3841 ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta aaactgatgc
     3901 tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta atcaaacccc
     3961 aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac cctcagaccc
     4021 atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat gatgtgctgg
     4081 aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg gctcacttga
     4141 ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa gtggtttcaa
     4201 atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag tgagaagtat
     4261 gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa gaatatgcta
     4321 aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg attgttgagt
     4381 gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa aggaatatct
     4441 gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa ggtaccggct
     4501 tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt aaatactaaa
     4561 ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa atttgttatt
     4621 ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa acgttacaag
     4681 aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac acgaactgaa
     4741 gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga ggagcgaatg
     4801 tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg cggctttctt
     4861 ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc attattatcg
     4921 atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt caaggagcag
     4981 aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc tctgaactga
     5041 tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg gatatgtcct
     5101 taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct aatctgagaa
     5161 gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa ctgctccagc
     5221 ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa cagaagaaaa
     5281 agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta cgaaaagtgc
     5341 agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag caagaagaaa
     5401 tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa agagaaaaac
     5461 ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca ctgacgaata
     5521 atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa gggatcgccc
     5581 atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac tatttgctgc
     5641 aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat gagagtcgtt
     5701 tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa tacggcgaat
     5761 ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct tcttctgatt
     5821 tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag cttctacttc
     5881 agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa ttggaaaaat
     5941 ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag gtacgtttct
     6001 taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa ttttacatta
     6061 cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt ggcgaactgt
     6121 ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg acaagtatta
     6181 tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc gcagataaaa
     6241 tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct caagttgctg
     6301 cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt acgcaaactt
     6361 ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg ctggctggaa
     6421 gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc gcaaaaagga
     6481 cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat atttt
//
LOCUS       DAZUMO010000113         6449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000113 DAZUMO010000000
VERSION     DAZUMO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.117"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..898
                     /locus_tag="ACG8ER_002686"
     CDS             41..898
                     /locus_tag="ACG8ER_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5051206.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            918..1478
                     /locus_tag="ACG8ER_002687"
     CDS             918..1478
                     /locus_tag="ACG8ER_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFS5051207.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2533..3300
                     /locus_tag="ACG8ER_002688"
     CDS             2533..3300
                     /locus_tag="ACG8ER_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFS5051208.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3326..3871
                     /locus_tag="ACG8ER_002689"
     CDS             3326..3871
                     /locus_tag="ACG8ER_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051209.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3872..4438
                     /locus_tag="ACG8ER_002690"
     CDS             3872..4438
                     /locus_tag="ACG8ER_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051210.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            4453..4701
                     /locus_tag="ACG8ER_002691"
     CDS             4453..4701
                     /locus_tag="ACG8ER_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051211.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4721..4960
                     /locus_tag="ACG8ER_002692"
     CDS             4721..4960
                     /locus_tag="ACG8ER_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051212.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            5331..5612
                     /locus_tag="ACG8ER_002693"
     CDS             5331..5612
                     /locus_tag="ACG8ER_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5051213.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            5602..5943
                     /locus_tag="ACG8ER_002694"
     CDS             5602..5943
                     /locus_tag="ACG8ER_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5051214.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         2399 a          899 c         1297 g         1854 t
ORIGIN      
        1 aataaattgc ttgaagtatt atatttagat atcataattt atggaagaaa aattaattaa
       61 aaaggatgag attataatgg ggttactggt tccgagagct aatacttata ctataccttt
      121 aacgaaacgc caattaattg ctaaaagata tcaaaggatt actagagcca tcaatagaga
      181 gttttggaat tctgaaagtg atactgctca cagtttatac gtaggttctt atgggagagg
      241 aacggcaatt agtactagtg atatagatat aatagttgaa ttaccgatgg cagaatttga
      301 cagattcaaa aattatttat caaatggccc gtctaagtta ttacaagtta taaaaaacgc
      361 atttcaggaa atactaccaa atagtgatat tagagctgat ggacaagttg ttaaaataaa
      421 tttccatgat ggaatcaaat ttgaaatagt tccagctttt aatgaaaaag attattgggg
      481 tgagtcaaaa gggtttattt atccagattc aaacatggga gggaactgga aagctacaaa
      541 tcccaaaaaa gagcaagaag ccatgaaatt aaagaataca aaaagtaata atttattata
      601 tgctacgtgt aaacattttc gacatgtacg agatacagaa tttacgagtt atcatctttc
      661 aggaatagta attgacagtt tcgtatatga ggctatggga aattggaaat tcgttgaaaa
      721 taacagtggg ggtcaaaaca ttagttctgt atcttatgag acagctttat tagaatacta
      781 taactcacat aaagttatgg gcggtttaaa tttatactct cctggaagca atcaatttgt
      841 taattctgat agtagtatca tatgtttaga aaaagtatta aaaaaaatag ctttataaag
      901 ttttatggag gaacaacatg ggagacgaaa aaaagcgctt ggaagttcaa ttacgtgaag
      961 catatggacg agtaacgtat acttacacca ctcatttaaa gcaagccgat aatattattt
     1021 ctgacaataa aattttaagg tgtcttcaaa ttggtttgtc agcccttact actggagggg
     1081 tacttagtac cattattact gataatagtg agttaacctt tgttgctgga ttatgttcaa
     1141 caatttctct tggattaaac ttatacttta agaactttga tcttgctgaa gaagctaaaa
     1201 atcatcaaat tgttgccgat aaattatggt tgatcagaga agaatacgta agcttactaa
     1261 cagatttaaa tcgtttaagt gttgaagata ttgtgaaaaa aagagatcaa ttaatgaacg
     1321 aaactcatga agcatataaa aatgccccta aaacaaatag taaaagctat gaatcagctc
     1381 agaaagcatt aaaatctgaa gaagagcaat tttttacaga agaggaattg gataagatgt
     1441 taccagagca tcttagaaaa aagagttcta aatattgaac tcttttttta tgcttaaaat
     1501 tctcttgtaa tttttctatt caatcaataa ataatctata tttaagaaga aatatgatga
     1561 tagaaacatc tcctgaagcc accgcaagcc gttctaagca tagcttagcc cgacattgct
     1621 agatatgctc atctcaccat gaacgtttcc taaagccacc gcaagccgtc ctagccattg
     1681 cctagtccga catcgctaga ttagtgcatc tcgccataaa agttttctaa agccaccgca
     1741 agccgttcta agcatagctt agcccgacat tgctagatat gctcatctca ccatgaacgt
     1801 ttcctaaagc caccgcaagc cgtcctaagc atagcttagc ccgacattgc tagatatgct
     1861 catctcacca tgaacgtttc ctaaagccac cgcaagccgt tctaagcata gcttagcccg
     1921 acattgctag atatgctcat ctcaccatga acgtttccta aagccaccgc aaaccgtcct
     1981 agccatcgcc tagtccgatt ttgcttgatc ggttccattt ctgtttattt agtgggtcaa
     2041 caccgtaatc ttaaatagcc atcgcctatt taagattact aggtctgctt tgaaggtaaa
     2101 aagtctttca ttcttcaaga cttttaggct ttcaagctac gcctgaaagc attgaaattc
     2161 atcgctcttt atttggtttt gccatttata attccttacg cccattatct ttttcgcctg
     2221 tcttgccttg tcagaagtgt ttagagctta tctcatcttt ggattaatac aagtggcgta
     2281 aatttttttc taaacgcaag cgttacgaaa ataatttact taaacacttg tattaatccg
     2341 gcagataaga acgcattcta atcactcccg acaaggcaag acaggcgaaa aagataaata
     2401 aatgaacgaa aggaagaaac ataaaatgca aaaccaaata gcgatgaaga aaaaaccacc
     2461 tccctctaaa acaatccaaa caaaaaagaa ttttgaaaaa ccaaaaatca aaaacagaaa
     2521 gaagcgttca acatgagaga tagaatttta gaagttaatc aagaaatggt aaacaagtta
     2581 acaaatatca gtgggaacta tgaaaagtac gatggtactt tacttgataa ctatattttt
     2641 cacgatacac aaaatatcag aattggcaga agtaaaccga gagcgttcat tctcatcaaa
     2701 gagaaatatt tgaatgaatg gaatagcacc tatgaaatgg ttatgactga taactttgaa
     2761 aaggtattgg atttcagaaa tgctttgttg ggttctgata tgggaaatag cagtcttgaa
     2821 tctattgaag ttgaaaacaa gaccgagaat gaaggtgaag gattgattaa gaatgtcttg
     2881 aaaatcgaac aaaaatatat cagtgaagat agaagttttt atagagtcac aagctcggtt
     2941 ggtctagcaa actttgctac taacttaatt ggaaacaatg ctagtgaatc tttgctagtg
     3001 atgtgtctga atacaaagaa tgaggtcatt gcttatagcg aagttttcaa aggtggaatt
     3061 aatcaatcag tagcgatacc aagagagatt tttcaactag ctttgctaaa caattcagca
     3121 aaaatcgcag tatcacataa tcatccgagc ggatatgaat ttccaagtga aaatgacaaa
     3181 agttttacta cgaatttgaa aaacgcagga aaaacaattg gtattgaatt gttagaccat
     3241 atcatcgtaa caagtaaaaa cacaagctat tactcatttc aagaaaatgg ttctttataa
     3301 aaattagaga aaagaggaaa acaagatggg cgaaagaaca caagcgttaa ttacagtcaa
     3361 atatggtaac agcaaaacga aagcaagcgt gtcgattcat tatcagtggg ggtatggtcg
     3421 atatatgctg atggacgtat tgaatcaagg aatttattta atcaataaag catacgactt
     3481 acgagataaa tcaagagaag aattagcaaa aggaatcttt gatagaagta caggagttat
     3541 caattttgtt acctatgaat ctggagtgag tgcttatgat gaaacagggt ttggtgggcg
     3601 ctatgaacct tatcaagaaa cgattgaaga tgtgatgaag ttttcagata acaatgatgg
     3661 ctatgctcat ttagagatgg tgttcgatga aaatggattt agtctagatg ataaaaccag
     3721 attgcgattg tttgattttg agggtcaaca gataggacta aacaagtttt gtgaaagcgg
     3781 aaaagagttt gctacgaaag attttattaa gggatacaga ttattgctag aaaattatgg
     3841 aataaaccta gaagaagaga gcgtgaaata agtgtttgaa aaaagaaaag accagttccc
     3901 taatttagaa gcgaaaagtc aaaaagtgat tgatggtgaa atgattgaat tattccaaaa
     3961 aagttatgga accatagaaa ctaccgcaag ttgcaaatac tactttcaag gaaagagaaa
     4021 cacgtttaaa ggctttgccg attctgatga agaattggca aagaaaatca ttgattggct
     4081 tgtcagcagt gaacacgtag caagagaaga taccgttaga acaacgatta atggggaagt
     4141 cctaacaggt actagatgtg tttgttgtaa tcatgaattt gtagaagatg aaggttgcta
     4201 ccgaatcaat gaatatggtg attttatttg ccagagttgc cacgagatag aatttccaac
     4261 cgaaagaagt tggtttgaat acatgctaca agattatgag atggaaaaag aaaatggtga
     4321 agtagttttt tgtaccaaag aggaattgag taagagaagc gatgatgaaa ttgaagattg
     4381 gtgtgtgtgg ttagtagggg cagatgattg cgttgcatat tacacaacag cagaatagga
     4441 gtgtgaatca gcatggaagg aatggatttt ctagatcatg aagatttggt tgattttggt
     4501 tatacatgga aaggaatggt aggtattagc agaagtcttg ctaatgcctt ttacgaaaga
     4561 aattatgcag tatatgttct ttatgatgac gatacagaaa gccttgttga cgaggaatat
     4621 aaattggatt tagagaatgt tttatatgga atagaaaagg aagatttagc aaaatatatt
     4681 ttttcatggt tgggtcaata agtgaaagga agatagaaac atgacaaaat ttaaaagtaa
     4741 gggtatgcaa tggtgttctt attattatgt tggaaatgat gaagatttat tgagaaaaac
     4801 ccaacgaaaa ggttatccag ataagatatt gaaacaaata ggttattaca tgccagaaga
     4861 gaggatagaa aaagaatttt gtgaggttct ttttgatagt gaacagctat tagttttgtt
     4921 aacccaaaat aaattgattt gccaatttga aaataattaa gatgaggtgg tgaaccatga
     4981 atgaagataa gatggtatta agtgaatatg ctagattgag aactattaat tctttagcat
     5041 ctcatttaag ttgttctttc ctaacaaagg tgctaaaatt aatttacaaa tgtgaagatt
     5101 ttgtacaaga gaagcacaaa gacaagccta ctgcgaatag gcttgtcttt ttttcattca
     5161 ttacgattcc gtatgtgtgg gttagcgaca atttgttcaa ttaccgtatg atatagcatc
     5221 ttaatttgat tagattactt gcaatgtagt ttattaagag tataatataa tcataaatgt
     5281 ggacaatttg agaaataatg agtgttttat actcaaaagg agtggatgaa atgaaggtca
     5341 aaaatccaac tgaaaaaagc agaatccaag tcggaataga caagaattta aaagagaacg
     5401 cagaaatgat attggaagag ctaggtttaa atcctacaac agcaataaca attctttaca
     5461 aacaagtagt cgctagaggt gagtttccag tagagataaa gctaagtgaa gaggagaagc
     5521 aaggaatcag attgcaacag ctaactaagg acatgccagt agatgttcta gatacagatg
     5581 aaaagttaga agagtggttc gatgaagcct aatgagatag taacggtcta tgttgcattt
     5641 acagataaaa atggtggtaa aagaagacct attttggtgg ttagtgataa agaagataga
     5701 gtagaattct ttggaatcac aagtcaatat gagaaaaaat cagaccaaat aaagagggtg
     5761 tattttccta ttgcagaatg ggaaaaagca ggacttaaaa agcaatcatg gattgacgta
     5821 gggtctttaa aggctattcc caaaagccga gagaacatat cattcaaaaa ggttggtatg
     5881 ttatcagcta gtgatacgaa aaagctaaat aggtttatcg agaaattaca aagaaaagtc
     5941 taacaaggct tttctttttt gtttatatgt atattaagat atacgtttat ataaacgtat
     6001 ttatgtatat gaaaatatac gtaaatatat acgtgattat aaacattaag aaaacgttaa
     6061 gaattgttaa atagtgatat ttagtaatat acgaattata agatatattt tatatagaat
     6121 gtatgtaaat aaataaaatg tctgacggat attgaatagc aataaataac aataattcag
     6181 acggatacag tgagtgaagt agcttgctac gaacgaacag gcagaatgaa acggcagttt
     6241 cattagagca agtcgcttgc gacgtagcgt taccgctgat gtaactggca agttacatag
     6301 agagagtttg cttgcaaacg aacgttacct acttgaatag aaataaaaaa taacagatta
     6361 gtgaattttg aataggagcc acaactcaac caaccgcttg cggtgggtga gtcccacgga
     6421 ggaaaagacg aacgaacggt cgcgtagcg
//
LOCUS       DAZUMO010000114         6434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000114 DAZUMO010000000
VERSION     DAZUMO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.118"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(434..976)
                     /locus_tag="ACG8ER_002695"
     CDS             complement(434..976)
                     /locus_tag="ACG8ER_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFS5051215.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(1289..1840)
                     /locus_tag="ACG8ER_002696"
     CDS             complement(1289..1840)
                     /locus_tag="ACG8ER_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5051216.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            2157..2555
                     /gene="merR"
                     /locus_tag="ACG8ER_002697"
     CDS             2157..2555
                     /gene="merR"
                     /locus_tag="ACG8ER_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFS5051217.1"
                     /translation="MAYFISSFSEKCGVNKESIRYYERKNLLPEPDRTNSGYRVYSEE
                     DVKRVKFIKHLQEIGFSLNEIYKLLGVVDKDNIRCEGMFEFVSKKEREIQKKIENLNR
                     IEYMLKDLRARCPDEKELYACPIIEVLIEN"
     gene            2569..4209
                     /gene="merA"
                     /locus_tag="ACG8ER_002698"
     CDS             2569..4209
                     /gene="merA"
                     /locus_tag="ACG8ER_002698"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFS5051218.1"
                     /translation="MNQFRMRIQGMTCTGCEKHIEKALESMGALNAKADFSQSEAVFQ
                     LPDDYAIENAIEAVKKINYQPEEVEKLPVQKRVMTNNKEDYDLLIIGSGGAAFSAAIK
                     AVEYGSKVGMIERGTVGGTCVNIGCIPSKTLLRAGEINQSAHVNIFEGLHTSTGEVEL
                     DRLVDQKNELVDDLRKQKYTNLIDDYGFDLIKGEAKFIDENTVTVSGQTYSAQRFLIA
                     TGASPLLPRITGLKEVDYLTSTTLLELRKVPKRLTIIGSGYIGRELGQLFHNLGSEVT
                     LIQRSKRLLKEYDPEISEAMEKALIEQGIKLISGATYERVEQDGNIKKVHIIVNGKNK
                     VIESDELLVGTGRKPNTEVLNLSAAGVQVGPNNEIKINAFAQTSNRKIYAAGDVTLGP
                     QFVYVAAYEGGIAAGNAIGGLNKAIDLSVVPGVSFTSPQFATVGLTQQQAKEKGYEVK
                     KAVLSLENVPRAIVNHNTTGVFKLVVDRKTQKILGVHIVSENAGEVIYGASLAVKFGL
                     TVADLKDTLAPYLTMSEGLKLAALAFDKDISKLSCCAG"
     gene            complement(4734..5219)
                     /gene="vanZ-A"
                     /locus_tag="ACG8ER_002699"
     CDS             complement(4734..5219)
                     /gene="vanZ-A"
                     /locus_tag="ACG8ER_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS5051219.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5372..6283)
                     /locus_tag="ACG8ER_002700"
     CDS             complement(5372..6283)
                     /locus_tag="ACG8ER_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFS5051220.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2108 a         1137 c         1149 g         2040 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgataga ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaataaccca catgaatgat ttgaaatcgt
      361 cttaaaatgc tttattttat aacccaaaat agaataatta cttcaaaatg acgtcgcata
      421 tcactattaa tgattaatcg atgacttcca taatcatttt aagttccccg gatgttaaat
      481 cctcgctaaa aatgccagta actttttgtc caggtccaat ttcctttgat gataggttta
      541 acaaagagat agggatatag attaatccgt tttgtttcaa agacagagcc aatatttttt
      601 tccttgttcg aatagctgca tcatagcgaa gagaagagta aacacctctg ccaacaatag
      661 aaatatgctt ccccaataat aaatcaaggc tacctattac ttgtaatttc tgaaaggtcg
      721 tcccatccgt tttgatttga atatcctgta aatttatttt cctgtctaag gcatcttgaa
      781 aaaacaagtg tgtgcctctg cgataatcaa tgccacagag gtgcataaaa ttagttgtcg
      841 aaaaaataat cgctaccgtt gagacttctc cgacaaagga tactttttta tctacaaaat
      901 gttcttgtaa gagatgagca gccttataaa tcgcgtttaa ctgtctggta atttgggttg
      961 tctcttttat agacatgcgt ttgtattctt tttttgccgc gtgcgaagaa tgctcctttt
     1021 tcccaataaa aaaagctgct caaaagagca gccttttgta caggtttagt tttggtgctt
     1081 gccacccgcc ggacccttac gttccggatt tgtatcgagt ttagttttgg tgctcgccac
     1141 ccgccggacc cttatgttcc ggatttatat caagttgact atgagcctgc tctcatttgt
     1201 atatataata taccaaaaaa aggttgattt gtccaatact gtgggtgatt ataaccgtct
     1261 tactgtgctt atctattata taatacaatc aaaaacctaa ccgaaaattt aataacgttt
     1321 cactgatttc atcttcatta attccgatgt agcgtttggt aatcttctcg ctgctgtgac
     1381 caaagatttc cattaaggtg gccacgtctt tggttttctt gtaatagtgg taaccaaagg
     1441 tttttcgtag ggtgtgggtg ccgatatcgt cccttcctaa cagcttcgcg accttttgaa
     1501 acatctggta gaccgtattg acttctaaat ggccgccctt agtactggga aacaagtaag
     1561 cctccggatc taagggtgcg gtatagccgt gaatcaagtc ctgtaagctg cttaagtata
     1621 aaatgcgtgt ttttcctgtc ttttgctcca cgatgcgcgg gttttttgag gtaatcacgt
     1681 cttttttctt gagtttaacg atgtcggaca tgcgcagacc gctgttaatt ccaattagaa
     1741 acaagaaaac gtcacgttcc gcatttttat tccgtcgcag gcaaaataaa aaatcattaa
     1801 tctcctgttg cgaccgtagt ggctgaacat tatagcccat gacaatccct cctttttagt
     1861 ctgttcgaat acatgattat agttattaca acacgaaatc gtgtgtttag ggggagtaaa
     1921 aaaaaagaac cctataagga taggattctt gatacatgat tttatgtaat ttcatgtatt
     1981 taggtccgat attatttatt ataaaagaag tctcaaaatt tattggtacc acatttaatt
     2041 ttcacttcat ttttgaaagt aaataagaag gaaaatgata ttcccccctt cgtattattt
     2101 ttggttggac atgtacctaa gtacatggtt tataattaaa aaaaggatgt gatagcatgg
     2161 cttattttat tagctccttc tctgaaaaat gtggtgtaaa taaggaatcc attcgatatt
     2221 acgaacgaaa aaacttacta ccagaacccg atcggactaa ttccggttat cgagtttatt
     2281 cagaggaaga tgtaaaacga gtaaaattca ttaaacacct acaagaaatt ggcttttctt
     2341 taaatgaaat ttataaatta cttggtgttg tagataaaga caacattcgt tgtgaaggta
     2401 tgtttgaatt tgtctctaag aaagaaagag aaattcaaaa aaaaatcgag aatttgaata
     2461 gaatagaata tatgctaaaa gatttacgag caagatgtcc agatgaaaaa gaactatacg
     2521 cgtgtcctat catagaagta ttaatagaaa attaacgggg gtaggaaaat gaatcaattt
     2581 agaatgcgca ttcaaggaat gacctgtact ggctgtgaaa aacatataga aaaagcgctt
     2641 gagagcatgg gagctctcaa cgctaaagca gatttcagtc aaagcgaagc ggtatttcaa
     2701 ttaccagacg actatgcaat agaaaacgcg atagaagcag ttaaaaaaat aaattatcag
     2761 cccgaagaag tagaaaagtt acctgttcaa aaacgcgtga tgactaataa caaggaagat
     2821 tatgatttgc tcattatcgg ttctggtgga gcagcttttt cagcagctat aaaagcagtc
     2881 gagtatggat caaaggtggg catgattgaa cgcggaactg taggcggaac ctgtgtgaac
     2941 attggttgta tcccttcaaa aacgctgctt cgagctggag aaatcaatca atcagcacat
     3001 gtaaatattt ttgaaggatt gcacacttct acgggagaag tagaattaga ccgcttagtc
     3061 gatcaaaaaa atgaactagt tgatgattta cgcaaacaaa aatacactaa tttaattgac
     3121 gactatggtt ttgatttaat taaaggagaa gcaaaattca ttgacgaaaa cactgtgaca
     3181 gttagtgggc aaacatattc tgcccaacgg tttttaatcg caacgggtgc ttctccactc
     3241 ttacctcgta ttactggact taaagaagta gattatttaa ctagcacaac cctccttgaa
     3301 ctaagaaaag taccaaaacg attaacaatt attggatcgg gttatattgg aagagaactg
     3361 ggacagctct tccacaatct gggttctgag gttaccctta ttcaaagaag caaacgactt
     3421 ttaaaagagt atgatccaga gatttctgaa gcaatggaga aagctttaat cgaacaagga
     3481 ataaagctca tttccggagc aacttatgaa cgagtagagc aagacggaaa tattaaaaaa
     3541 gttcatatta ttgtaaacgg caaaaataag gtcattgagt cagacgagct actagttgga
     3601 acaggaagaa aaccgaatac agaagtattg aacttgagcg cagcaggcgt tcaagttggg
     3661 ccaaacaatg aaatcaagat aaatgctttt gctcagacaa gtaatagaaa aatttatgca
     3721 gcaggagacg tgacattagg acctcagttt gtctatgtag cagcctatga aggaggaatt
     3781 gcagcaggaa atgcaattgg tgggttaaat aaagcaatcg atttatcggt agttcctgga
     3841 gtatcattta ccagtccgca atttgcaact gttgggttaa cacaacaaca agcgaaagaa
     3901 aaaggatacg aagtaaaaaa agcggtattg tcattagaaa atgttcctag agcgattgtc
     3961 aatcataata cgacaggggt gtttaaactt gtggttgata gaaaaacaca gaagattttg
     4021 ggagttcata tcgtttctga aaatgcagga gaagttattt atggagcatc cttagccgtt
     4081 aaatttggat tgaccgtagc tgatttaaaa gatactttag cgccttatct aacaatgtca
     4141 gaaggcttaa agttagcagc tctagccttt gataaagata tttcgaaatt atcttgttgt
     4201 gcaggttaat atttctattt agtttcttgt agtagctggt aacaccttgg tttatctttc
     4261 gttattatct ataacgatag gagttgaaga ttatgacatc agcaaacgtc tacgtgccaa
     4321 ttaatagctc tattctagtg gaggttgaag aaataaaaga ctatttagaa acgcaattgc
     4381 tcatttaggc aacgcagcaa gatattattg aagatgcgat tgttgcctta cgtgcttaat
     4441 atagataatt aaatcgtatc aaaaaaggat tctaccgctt aaaggggtac cgtcaggaaa
     4501 atgcggattt acaacgttaa ggaaatttat taaagagacc aattcctcag cactaaaatt
     4561 gataattggg ctttctttgt tactatagtg ttaggtaagc tttttacttt atcaccggtt
     4621 ttcccctcac ttcacaccta cgggcgagtt tccccacatc ccgttttcca tctaatccaa
     4681 ttcattcaat atctttatgt gtatgaaaga tcagattgct tgatttagac atcttacacg
     4741 taatttattc ttaaatgggt acggtaaacg agcaataata cgagcaaaag tatacctaca
     4801 aaaataataa ctctgtctaa ttttttttga ttcatatgct tattgcttaa accatataat
     4861 ttcagtccaa gaaagcctcc aacagtattt gtaattacat ctgttatgtc tgtcgctcca
     4921 atagcgaaga taaattgaat tatttcaaaa gtaagactta aaaccagtac aaaagcaaac
     4981 ttaggtaaaa atccgatttc tttaaaattg acattcaaaa gcaagccaaa tggaataaag
     5041 attataacat tatctatcat ctctctgaaa ttcccagtag cagtaaatgg agtcaagtta
     5101 agacttcttt gatgataatt aaatactgat aaaatattgt attgtaattt gaataacact
     5161 aaccagatta gtgtcactaa atataaagct agcaatcctc tagataatat ttttcccaat
     5221 ataatttcct ctaattcttt tgtttaagtt gtatcattag tatagcaatg tcctagttaa
     5281 cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt gctagtacaa taatgctgtt
     5341 cattaatttc gttggtttca ttccgccatc cttacctcct tgaattagta tgtgttgatc
     5401 cgggaaacac tgtcacaatt acaccgtcta tattgtttcc tgatatctca taacgaagat
     5461 tagtcggcac atgaatggtg gtatttttag taacaggata ataaaagatc tcatattttt
     5521 ccccatttac actaacagaa atggtttttt cttcttttag gtaatccata tattcctcga
     5581 gaacgaaatt cttttctttc ataatcgcac tatgtggtaa accaacatag cgaatatgcc
     5641 atggttcata ttgaattcct gttaactctg ttttgtcctc tggataacgt aaaatgaacc
     5701 cgtatttcca agcattttct tctatccact ttccttcagg ggctcgttcc attttcgtca
     5761 agcttgatcc tacatctagt gataaacctg aattatgctc actataacct gctggtaagg
     5821 catactcagc ccccatttct tggtaaagca cactttgctc atcaaagtct cgatagccac
     5881 tattaataat aaaatgacta acgccaccct ttacagcatc attgaccatc tctgaaaatt
     5941 tttgtgctat ttcttttgac atataaatat tactatcaag caacccgtat ccatttatta
     6001 attcgtcatg tttagataaa ttcacgatat ctgacttcac actttcttgg cgaacaggat
     6061 atttactatt gattaatagc agatttcctt gataaacctg ttcttctgta attgttttct
     6121 cttgggtatt ttcagaagtc ccactatttt ctaaatgttc tttgggattt tgatcataat
     6181 tttgaaattc gactttttcc tgagaaaaca gtgcttcatt aacgtagtca taacctaagt
     6241 atattaagaa taacaataac aataaaaaaa acaacttctt cattttcagt ctcctcctta
     6301 actatatagt tggaagtatg aaatatttct agaatcttcc ccttaaacgg cacaatctcg
     6361 tttgagattg tgccgttttg ccccgtaaat tacagtcaat tatttcgccc tactggattt
     6421 actattatca ccaa
//
LOCUS       DAZUMO010000115         6253 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000115 DAZUMO010000000
VERSION     DAZUMO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.119"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..1497)
                     /locus_tag="ACG8ER_002701"
     CDS             complement(19..1497)
                     /locus_tag="ACG8ER_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051221.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1795..2730)
                     /gene="murB"
                     /locus_tag="ACG8ER_002702"
     CDS             complement(1795..2730)
                     /gene="murB"
                     /locus_tag="ACG8ER_002702"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS5051222.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2803..3723)
                     /locus_tag="ACG8ER_002703"
     CDS             complement(2803..3723)
                     /locus_tag="ACG8ER_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5051223.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3836..4588
                     /locus_tag="ACG8ER_002704"
     CDS             3836..4588
                     /locus_tag="ACG8ER_002704"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS5051224.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5452..5985
                     /locus_tag="ACG8ER_002705"
     CDS             5452..5985
                     /locus_tag="ACG8ER_002705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5051225.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1880 a         1196 c         1043 g         2134 t
ORIGIN      
        1 agctgccatc cataaatact agctgatttt ttcagttaaa tgtctatttt tttctctgat
       61 gatggtattt ccaaaatatt cccagtcatc tggagtcggt ggatttttcc aaattatctg
      121 ttctttatta aatttttcaa gtaaacaatc agataaataa atattcgtac gagaagtgat
      181 tcgactctca atcacaatat tgtaattttt aaatcctttc acttgagaaa taatgtaatc
      241 ggtataagca gatccttgcg aaaaatcaat acagacatga atcggtgctt caaaatatga
      301 taccggacaa ataccgacca ataaaaagag ataatcataa aataagcgaa tcatgaaacg
      361 ttcatctttt cccaagaaat agcgtttatt ctcttgtatg accttttgga ttgctgtact
      421 gaatattgga tacgtctcga agaaaaactg tagctgcttt ttagacgtaa agctagtcat
      481 ctcaaagtcg aatatccaat tttttcgatt gacatgcatc atacccatac gaagttcttt
      541 tcgtatatat agtctttctt cttcagagat ttcaatcgaa aatggtaatt gatcaatcaa
      601 ttccactgac attttttgac taatcatgtg gatatcatga aaatgagctt ccgcaagtcc
      661 aaatgcgcca tctaattcaa attcttcttc cgtgatcaaa aacaacatga tataagaaat
      721 ctcatcaatg atctgctctt tagggatatg aagaaaagaa atatgtgata aatccttgat
      781 gatcttcatt ccttctgagc tcttaaagta ttcatctgaa aagaaacgtt ttccgattgg
      841 ttgattccca taggccctca acaaactaat agtggttagc aaattgaatt tgattcgttt
      901 tgttgcagac agttttaaat tgaacagata ttcaaaatat tgcacgatac tctttgcttc
      961 attcatgacg ctttctctaa atggcataga aaaaccatta aagaccccga aatatacccc
     1021 aaataatacg ttccgtatgt ttatttcttc cccaactaat atgtttttct ttaactttat
     1081 ttgatgctgt tcaagaaaag agactaattc tcttcttttt acataggctc ttgatatgct
     1141 taaaaaatga ttctctgcaa atttttcgat cgttactttt gaatccacga tctccgttaa
     1201 cagtcgagca atttttgatc gttgaaagta gattcttctt aaatttttga tatccgtcaa
     1261 attcagacgt tcgacatgga tctctgcttc ttctgaaatt tggatctctg gctttgtttt
     1321 gaattcagta aattctttat ttaattctat gatataattt cttgttttaa atttagacaa
     1381 acctaatgtt tcatataact taggcagcac tacataattc tttttctcaa attctaaata
     1441 tctagctagc agcagttttt ctctatcttc ctcttctata aggtaatcca acttcatatc
     1501 ctacacctca aaaaatgcct cataccaatt acttgatatg cagatgaaag ttcatctttt
     1561 cacctatact gattttatga tggattatgc ttgttttttc atcagttatt ctcatttttt
     1621 atcttttttt tgagaatatt gcaaaaaatg gaaaaattta tgaggaaata ttctatattt
     1681 tttgttttat tgtgttaaaa tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa
     1741 agagcaggac ggccaccgtc ctgctctttt acttatttta agttccatta cttattaatt
     1801 ggctaaactt ctgcttgttt ctgatattgc ttgtttttct ccaatgatcc gcacttctgt
     1861 ttctaaagac acatcaaatt tttctttgat tacttcttgg atgtgcgcaa tcaattcaat
     1921 ataatctgtc gctgtcgcat gatcgatatt gacgatgaat ccagcatgtt tttcagagac
     1981 ttgtgcaccg ccccatttca ggccttgcaa tcctgcttgt tggatcagct gacctgtata
     2041 gtgtcctact gggcgcttaa agacacttcc gcatgaaggg tattctaatg gctgttttga
     2101 ttgacgcaat tcggtcaact catccattct tgctttgatt gcttcataat cccctgtttg
     2161 caaactgaat cgagcttcca atacgatagc acgtaattct tggactttac tgtgacgata
     2221 agaaaatacc atttcgtctt ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc
     2281 cacttcagca aaaacatctt tgatctctcc gccatacgca cctgcattca tataaaccgc
     2341 tccaccgaca cttcccggga tgccgcaagc aaattcaaat ccagtcaatg attctgccaa
     2401 agctgcataa gtcgtatcaa tcagtgaggc acctgcttcc gcaatgacca tcgttccttt
     2461 gacatgaatc tgtttcattt cagtcagcat gatgactaca ccgcgaatcc cgccatcttg
     2521 aacgatcaga ttgcttgcat ttcctaatac catccaaggt atatcatgga ttcggcaata
     2581 atcgatgatt tgctttactt cttcttgttt tttcgggaat gcaaggacat cagctggtcc
     2641 acctgttttc gtaaacgtat agttcatcaa tggttcatca aacaatatag taatcgtggc
     2701 taagctttca actatttctt ctttattcac tgaggatttt cctttctttt ctcaaacgtc
     2761 ctcattattc tatcacgctt aatacaatct tgccagtctt gttcaaagtt gatgacggta
     2821 gtgatcttta agcacttcct cacataattc atgacttctt acaacttctt tttgtttaag
     2881 caattctgtt tccgatttac ctaaacggat ctgagaaaag aaggcctcta ccattggctc
     2941 aaacccgcgt ttgactagcg tgtttgtcca gtcgccaaat tctttcactt tttttccatc
     3001 agcatcttga atgacaagct gggtcagatc gtttacttca taagtaccat ttttacttgt
     3061 tacttgatat ttttctatat tagcgccact tgctaaatcc atcgttaaaa tagccgtttg
     3121 attttgtgtt tctaattgta aaatggcata ttccaaaaat tcctttgttt ctcttatctg
     3181 gtattttgtt ctcaaaatgg gttcatctaa taaatacaca gctgtatcaa taagatgtaa
     3241 aaacaaatca taaaggacga actctgtcgt ttcttttgcc gctattcgat ttttttgcag
     3301 gtggatcaac cgcttatctg ggatacgttt caattcttct accataggag caaagcgccg
     3361 gttgaaaccg atcatcaaaa ttatttggtt cttatcagct aattcttgca attcacgaac
     3421 ttcatttaga tggacactca gtggtttgtc aatataaaca tggataccat tttctagaca
     3481 ttttttggct aattcataat gtacttttgt tgctgcgtgg atcatacatg cttcgatttt
     3541 ttcttctaaa agctcatcga tcgtttcata tagatgttgg aacccgtact ctttttgaat
     3601 tctttctttt gtttctttat ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt
     3661 cgcataagtt ggtaaatatg ctttttctgc gatattccct acaccgatta caccaatctt
     3721 catctacttt cctcctggat catttagtta ctttcatctt acaccatttt ttactaaaat
     3781 gaggtacaat agtatcgcaa aggtaaggaa attaattcga aggagtgtgc ttttattgaa
     3841 atttatctct tggaacgtaa acgggcttcg tgccatcgta aataaaaatt ttttagaagt
     3901 ttttcatgag ttagacgccg attttttctg tctgcaggaa acgaaactcc aagcaggaca
     3961 aattgattta gatcttccag gttattatca atattggaat tacgcggaac gaaaaggcta
     4021 ttccggcaca gcgatttttg ctaaaaaacc agcattgaat gccacttacg gaatgggtat
     4081 cgatatccat gatacagaag gccgtctgat tactttggaa tattccgatt tctttcttgt
     4141 aacctgttac acgccaaatt ctcaaagcga attaaaacgt ttagattacc gattagaatg
     4201 ggaagaagcc ttttataatt atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg
     4261 tggggatcta aatgtcgctc atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa
     4321 aaatgctgga ttcactccag aagaaagagc ggctctttct cgactgttgg ataacgggtt
     4381 tatcgataca ttccgttatt tttatccaac ccaggaaggt gtctactctt ggtggaacta
     4441 ccgattcaat tcaaggaaaa acaatgctgg ttggcggatc gattactttt tgacgagcaa
     4501 agatttagag ccaggactag ctgatgcaaa gatccatacg gacattatgg gcagtgatca
     4561 ttgtccagta gaattagatt tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt
     4621 attaagaaaa attttgtgaa aatggctttc cacattttgc ataaatttgg cataataccg
     4681 agaaactggc ttttgaacac tgtttatcga gagattgtga aaaagctgtc ctgcatcaag
     4741 taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg ggcttattac
     4801 cgcagataca agcttttgaa caccgtttat taagaggttg tgaaaaagct gcactgcatc
     4861 aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg ttgggcttat
     4921 taccgcagat gcaagctttt gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc
     4981 atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttggact
     5041 tattaccgca gatgcaagct tttgaacacc gtttattgag aagttgtgaa atcagcgtcc
     5101 tgacctgagt attagagaaa caaatggcaa aatagttcct catattttgc tattttttga
     5161 gctaataccg agggtcagct gattgaacac cgtttagtgg agaagttgtg aaaaagtcgc
     5221 ctactttcga gtcgtaagaa atttttttga aaatggttcc tcacaacttt gcataaattt
     5281 gacttaatac cgagaagtca tgatccccat ctaactccaa atcacaagga atgagttcaa
     5341 aaactagctt ttctcaacaa atattacata aaaaattcta tgttttttgt taaatttatt
     5401 tgcttttttc aaaagtatgt tacagtaatt tcccaaggag gttttacaga aatgaatttt
     5461 gttgccatgg attttgaaac tgccaatcat cagccttata gtgcttgttc tcttgcgcta
     5521 gtcatggtaa aaaatagcca gatcgttgat gaattctata cattgataca gcctgagact
     5581 ccttttttct ggcgaaatgt ccaaattcac ggaatccatc aagaagacgt acgcaatgcg
     5641 ccgaaattcc cagaagtctg gcaaactatc caaccatatt ttcaaaaaaa tcgtttagtc
     5701 gttgcacata atgctgcttt tgatacaaag gtcttagcgg gatgcttaga ttattatcat
     5761 cttgaacagc ctaattatct ttctttatgt acagtgaaaa gtagccgtcg cctgtttcct
     5821 gaatttccaa atcatcgatt gaacactgtg tgcgaaaatt tgaacatcca attagatcat
     5881 caccatgatg cattggaaga tagtcgtgca tgtgcggaaa tcctgcttta tcaagaaaaa
     5941 aaattcggga cagatccatt gaaaaaactt gtgactataa agtaaaagaa tacttatgaa
     6001 acacacaaag aggtcgtgag cctgccgttt cactccgagt cacaaggagc aatttcaaaa
     6061 aatagcttct catgtttatt aaatattgtg actggtgacc gaggagctgg caggagaaca
     6121 ccgtttattg gagaggttgt gaaaaagctg ttctgcatca agtaataaga acagccaaac
     6181 aaaaatagct tctcatattt ttcatttggt tggacttatt accgcagatg caagcttttg
     6241 gacaccgttt acc
//
LOCUS       DAZUMO010000116         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000116 DAZUMO010000000
VERSION     DAZUMO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.120"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8ER_002706"
     CDS             725..811
                     /locus_tag="ACG8ER_002706"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5051226.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8ER_002707"
     CDS             complement(932..1621)
                     /locus_tag="ACG8ER_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051227.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACG8ER_002708"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8ER_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5051228.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8ER_002709"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8ER_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5051229.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8ER_002710"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8ER_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5051230.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8ER_002711"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8ER_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5051231.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG8ER_002712"
     CDS             complement(4578..4748)
                     /locus_tag="ACG8ER_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051232.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8ER_002713"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8ER_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5051233.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8ER_002714"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8ER_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5051234.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1632 a         1151 c         1042 g         2348 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga atgactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZUMO010000117         6161 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000117 DAZUMO010000000
VERSION     DAZUMO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.121"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8ER_002715"
     CDS             <1..355
                     /locus_tag="ACG8ER_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051235.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..1076
                     /locus_tag="ACG8ER_002716"
     CDS             615..1076
                     /locus_tag="ACG8ER_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFS5051236.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            1120..1206
                     /locus_tag="ACG8ER_002717"
                     /pseudo
     CDS             1120..1206
                     /locus_tag="ACG8ER_002717"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1172..2242)
                     /locus_tag="ACG8ER_002718"
     CDS             complement(1172..2242)
                     /locus_tag="ACG8ER_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5051237.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            2844..4688
                     /locus_tag="ACG8ER_002719"
     CDS             2844..4688
                     /locus_tag="ACG8ER_002719"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5051238.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            4744..>6161
                     /locus_tag="ACG8ER_002720"
     CDS             4744..>6161
                     /locus_tag="ACG8ER_002720"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5051239.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSF"
BASE COUNT         2044 a          985 c         1247 g         1885 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taaacaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcgt
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aataagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtagatgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaatagagta tgtacccaat gagctaattg
      781 tcagtttaaa ggctacacaa agaatttcaa cagagaaaaa taaaccgcaa aatttattgg
      841 gtgtaaaagt tatggtggaa aaagatttga ctcttaggac aaatgaaagc gattataata
      901 agcgagatca agtattatta gtgaaattga acgaagaaat tagtgtggaa gaggcagtaa
      961 gaacatttaa tgcttctgat atggtcaatt atgcagagcc aaactatgtg gttgaactag
     1021 agccgttgaa tgatgttgag gagccaaata caactgattc tttaggaaat cgataacgat
     1081 cctaattgga gtaaacaagc taatttaaga gagattagca tgccatccgc ttgggaagaa
     1141 accaagggtt ctaaggatgt gaaggtagcg gtcattgatt caggaattga ttatcaacat
     1201 gaagatcttt ctggaaatgt agatgaaagt ttgggatacg atttttttta cttttttcaa
     1261 gaaaataata attcagaata ttctgactat atttatgcaa cactagtgac tttaggagaa
     1321 acatcaatag ccttgctaaa gtttttgctt tcgctaaaga gagtattttc gataatttgt
     1381 ttttttctaa aatacctcgt tcctcaatat ttgggatatg gatatgtata gggattcttc
     1441 gcataaaggt ttccagaaaa tcctctttgc cttctgtagt cgcaaaaact aaacgtacat
     1501 tggaggcgtg atggatagtt tcaccgactc tagaataagt tccgccatcc atataaacaa
     1561 atagtttctc ttgcccttca ggaggcaatc tgtgtacttc gtctaaaaac agtactccac
     1621 cgtctgcatc ttgcaacaat ccatttcgat cttcatctgc gccggtaaaa gcacctttaa
     1681 catagccaaa tagtatcccc gacaataatt caggattatt atggtattgc gcacaattaa
     1741 gtactttaaa aggagcattc tcatgaataa cgttcacttt tttcataaac tgataagcag
     1801 tatgaacaaa aaaacttttt ccaactccag tatctccagt gatcatcata ggaagccctc
     1861 ttccaggata gaagacagca gttttcattt gttccaccgc atcggataag ctcatatcat
     1921 acccaatcat cttttgtaga atggaatctg tagaattggg ctccaagtct gtttttaatt
     1981 cagatatact ttcgtataca tcttttaccg gaatcaaaaa gtgctcttca aagcgctctt
     2041 tgatggcaaa taacactgga cgagtattga ttttaaatag aacgttcttt gcaactaagt
     2101 cattggcaag tttactggca tttgttcttc ccgttcttaa aaattgcgac aggtcatttg
     2161 tgtcaaaaaa gcactcttct aaattactga tgccaatttc ttcacatttt gtgtttgcat
     2221 aagtcactat atcatttttc atcttataaa cagtcctttc taaacttcta tcccttaaat
     2281 aaatagcaaa aaaaaggaag caatatcgat atgctgtcct cttaatttaa taaagcgcta
     2341 atctaattca ctgtattatc attttttagt agtatagcac aattatgaat gccttttctt
     2401 tatatcaaat aaagtttata taaaaaacct catcctgcaa gaaagaaaaa atgaacccat
     2461 tctttttata ctaggtctta aaaagacaga acatctgcta gaagatagaa gagaataaga
     2521 gttatatatg ttggaagata ttcttagaaa aaagacaaaa cttttagaac acgaaaacta
     2581 tattgaacga ataaaatgaa aaaatgatta aaaaatcatt ttattattta cgtttacatt
     2641 ctaatatgtt aaattgaata taacttaaac aggcatgtga ctggtaatgc aggcaggacc
     2701 taaataagtt gaataacaat gtatgcgttt caatacacgt ttgccttgtt gtcaatttat
     2761 ttagtttctg cctgtttttt gtgttgagga tggccacttc gactcaaaag ttcatcactc
     2821 aaaaaagaaa agagggaaac accatgaatt acaaacaaac agcagaagaa gtattgcagt
     2881 acatcggtgg ggaagaaaac atatcccatt tggagcattg ctcaactcgt ttacgtttta
     2941 cgttaaaaga caacagcaag gtaaatatga gccagttaga aaaagtggat ggcgttatgg
     3001 gcgttcgcca aaatgtgcaa tgccaagtaa tcattggaaa cgatgttgtt gaagtgtatg
     3061 atgaagtgaa agcgttatta ggtgacaatg tttcaaatac tgaagaagca ccaaaacaaa
     3121 aacaaaaatt aggttcagtt ctattagatt ttattatttc tattttccaa cctttaattc
     3181 ctgcgattgc aggtggagga gtattgaagt ccttgttgct actagcttct atcttagggc
     3241 tgattagtga ccaatctcaa acctatcaga ttttaaattt ggtcggcggg gcaccgcttt
     3301 atttcttgcc tattctggtg gcaatgacta cggctaataa attgaaagtc aatcaattgg
     3361 ttgcagtatc tgctgtcggt gcattgatcc ttcctgaaat gacagctatg ttggcggaag
     3421 gtgctacatt cttatctttt ggattggata atatagcgta tgcatctcaa gtattcccag
     3481 ccatcttaac tgttttattt tatgctcaac ttgaaaaatg gtttacaaaa gtttcaccaa
     3541 aaccaattcg tattttcttt gtacctatga tgagcttatt gattacagtt cctattgctt
     3601 tgttgcttct aggaccatta ggttataatg taggaacagc tttctcttct atcattgtgt
     3661 ctttgtacaa tcaatttgga tgggttgcaa ccggagtctt agcagctgta ctgccattaa
     3721 tggttgtaac gggtatgcat aaagcaatga tcccttacgc tgtttcatcc atgagtgaat
     3781 tgggtgcaga acttctttat ctcccggctt cattggcaca caatatttca gaagctggcg
     3841 catgctttgc tgtcagtatc aaaacaaaag acaaaaaatt gaaatctacg gctgtttctg
     3901 cgggtatatc tgcattattc ggaattactg aaccagctct ttacggggta acgattctgc
     3961 acaaacgtgt tttatatgcc gttatgtttt ctagcctgat cgggggagca tttgcaggaa
     4021 tcgttgcact tcaagccttt gctttagtgg gtcctgggat tgcaagtatc acgatgttcg
     4081 ctgacaaagc tgacccaatg aatctggttt gggcatttgc agtatcgggg atttcatttg
     4141 tggttgcttt tgctgcagcg ttggttcttt ataaagataa agaagttgtc aatgaagttg
     4201 aaactaagat ggaaacggcc gttgtttcag atgctgttct ttcaagtccc gtgaatggaa
     4261 aagtaatctc actatcagaa gtaaaagaca ctgttttttc tagtggtatg gtaggtgatg
     4321 gagtagcaat cattcctgaa gaagggatat tagtagctcc tgaagatgga gaaatcacaa
     4381 tggtatttga aacgaagcat gctattggtt taaaaacggc taatggtgca gaacttcttt
     4441 ttcatattgg tattgatact gttcagttag aaggaaaagg gtttgaggca tttgttcgtt
     4501 ctggtgaaaa agtagccaaa ggacaaaagc tgattgcatt tgatattgat ttaatcaaag
     4561 aagcaggatt cgaccccaca gttatctgtg tggtaaccaa taaggataat tacactatcc
     4621 gttcatttaa cctagaaaaa ttggtaacca atgatagtga tctcttagct atcagtccga
     4681 tagcttaaac acaagatagt gaaaaaaaat atattgaatg aaggaataga ggagaataga
     4741 ataatgacaa acacaacaag ttttccaaaa ggatttttat ggggcggcgc gatagcagca
     4801 aatcaagtag aaggtgcttg gaatgtagac ggtaaaggtc tttcagtagc ggatatggct
     4861 acttataaac caaatttaga caacaaagat tttgctgctc atatgcatgt caccctgcag
     4921 tctataaaag aagctagtga agacaaatct gataaatggt acccaaaaag aaggggcgtt
     4981 gatttttatc atcactataa agaagattta gctttatttg ccgaaatggg ctttaaaacc
     5041 ttgcgcctct ctattgcttg gactcgcttg ttccctacag gagaagagct ggaaccgaat
     5101 gaaaaagggg ttgcatttta tgaaaacgtc tttaaagaaa tgcaacgttt aggaattgag
     5161 ccgattgtga cactatctca ttatgaaatg ccattagctt tagcagttaa atataatggc
     5221 tgggtggagc gtcgagtaat tgatgatttt gttcgcttcg ctaacgtttg ttttgatcgc
     5281 ttcggcaaat acgtaaaata ttggctgaca tttaatgaga tagacagcat ccaccgtcat
     5341 ccatttacaa cagctggaat tatccaagag aaaagcgcag aaggtcaaga agctcaagat
     5401 atctatcaag cattgcatca tcaatttgtt gcgagtgcgt tagtgacaaa agatgctcat
     5461 gaaaaaattc caggaagcca agttggttgt atgttaacga aattgatgac ttatccatta
     5521 acttgcgcac cagaagacgt tgagctgacc ttgaaaaaga acttagaaaa taatttttat
     5581 gcagatgttc aagttaaggg agaatatcct gctctaatcg aaaaggattt ggaaaatcgg
     5641 ggaattcaga ttaaaatgga agatgaagat cgagaaattt taaagaaaca taccgtagat
     5701 tttgtttctt tcagctatta catgtccatg gcagaatcag ctgatccaaa cgcagaacgc
     5761 acaccgggaa atactgtttt aggggttaag aatcctcatt tgccttcaac agattggggc
     5821 tggcaaatcg atccaaaagg tttgaaaatt tcgttgatcg aactttatga tcgttaccaa
     5881 gtaccgttga tgattgtgga aaatggtatg ggagcagtcg atcaggtagt agatggtaaa
     5941 atccatgatc cgtaccgcat cgactacttt aaagcacact tccaacaaat gaaagaagca
     6001 atcgatgaag gtgtagattt gatagcttat actagctggg gaccaattga tttagttagt
     6061 gcaggaactt ctcaaatgtc taaacgatat ggatttattt atgtggatgc agacgatgaa
     6121 ggaaatggga catataaccg cttgaaaaag gattcgttca a
//
LOCUS       DAZUMO010000118         6136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000118 DAZUMO010000000
VERSION     DAZUMO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.122"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACG8ER_002721"
     CDS             complement(207..1169)
                     /locus_tag="ACG8ER_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5051240.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACG8ER_002722"
     CDS             complement(1171..2610)
                     /locus_tag="ACG8ER_002722"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS5051241.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACG8ER_002723"
     CDS             2795..4741
                     /locus_tag="ACG8ER_002723"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS5051242.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /gene="scrK"
                     /locus_tag="ACG8ER_002724"
     CDS             5005..5877
                     /gene="scrK"
                     /locus_tag="ACG8ER_002724"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5051243.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1946 a         1055 c         1084 g         2051 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 atatctttgc ttcttc
//
LOCUS       DAZUMO010000119         6068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000119 DAZUMO010000000
VERSION     DAZUMO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.123"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG8ER_002725"
     CDS             832..1566
                     /locus_tag="ACG8ER_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5051244.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG8ER_002726"
     CDS             1582..2751
                     /locus_tag="ACG8ER_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5051245.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8ER_002727"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8ER_002727"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5051246.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG8ER_002728"
     CDS             3841..4767
                     /locus_tag="ACG8ER_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5051247.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG8ER_002729"
     CDS             4786..6036
                     /locus_tag="ACG8ER_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5051248.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZUMO010000120         6066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000120 DAZUMO010000000
VERSION     DAZUMO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.124"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            437..643
                     /locus_tag="ACG8ER_002730"
     CDS             437..643
                     /locus_tag="ACG8ER_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS5051249.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            643..894
                     /locus_tag="ACG8ER_002731"
     CDS             643..894
                     /locus_tag="ACG8ER_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051250.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            909..1346
                     /locus_tag="ACG8ER_002732"
     CDS             909..1346
                     /locus_tag="ACG8ER_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051251.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            1339..2046
                     /locus_tag="ACG8ER_002733"
     CDS             1339..2046
                     /locus_tag="ACG8ER_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051252.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            2211..2411
                     /locus_tag="ACG8ER_002734"
     CDS             2211..2411
                     /locus_tag="ACG8ER_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051253.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            2427..2606
                     /locus_tag="ACG8ER_002735"
     CDS             2427..2606
                     /locus_tag="ACG8ER_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051254.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            2947..3210
                     /locus_tag="ACG8ER_002736"
     CDS             2947..3210
                     /locus_tag="ACG8ER_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS5051255.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            3204..3554
                     /locus_tag="ACG8ER_002737"
     CDS             3204..3554
                     /locus_tag="ACG8ER_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5051256.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(3578..4192)
                     /locus_tag="ACG8ER_002738"
     CDS             complement(3578..4192)
                     /locus_tag="ACG8ER_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5051257.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            4506..4928
                     /locus_tag="ACG8ER_002739"
     CDS             4506..4928
                     /locus_tag="ACG8ER_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051258.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            4938..5141
                     /locus_tag="ACG8ER_002740"
     CDS             4938..5141
                     /locus_tag="ACG8ER_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051259.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            5352..5936
                     /locus_tag="ACG8ER_002741"
     CDS             5352..5936
                     /locus_tag="ACG8ER_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5051260.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         2138 a          944 c         1157 g         1827 t
ORIGIN      
        1 aagaagagtt gtagtcctaa atgaggattg cagctctttt tgttttacat agaaacagga
       61 aaagaccttt ttaggaagac acaagtgcct tctcaattga aaaacctttc tctcactcca
      121 accgccaccg ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg
      181 tcttgcaaag caagacaaaa tagacaaaac ttcctctcac caaaaggttc gttcctgttc
      241 gttatctctt tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga
      301 caaggacaag ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattta
      361 ttctggccaa caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga
      421 ggaggcagaa tcaaacatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt
      481 attcccaaac tatcatgcac cacatactgt tgaatgcgat tgtggagaaa gagcgaaatg
      541 tatcaaaagc tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta
      601 tggcgattac attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa
      661 ttattgactg taaaagaagt cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa
      721 gtagcagaag ctttacttat tccgcggtac tggacgatag aaaccccttt agtagaggat
      781 gcaccgacta tgctatttga agacctagtg aagattgcag gatatgaact gcattatcaa
      841 gaaaacatgg aaacttatga aatcgaatat tggttaacca aattagagaa ctaaaaagga
      901 gaaaaaatat ggctcaaaat tactatgacg aatttgtcaa attacctttg gataaaatgg
      961 cacaaaaaat ggaggatatg acgttcctat accatgaaac acgtgtaccc aaaaaacatt
     1021 acaaggaaaa attgtcagtc gcagtggaag aaatgattga atcaggtgtc gaaatgaatc
     1081 tcattgcgac gtactatcga acactggaag aactgaaaaa acaaaatgga aaatggtttt
     1141 tccaagcctt actatgcttg gaagtaggtg tcaaaccaag cactatcaaa ccttccgaat
     1201 atcaagcctt agaactgact tatgcaaaat tcgcacagat taagaaagct aaagcaatgg
     1261 ctagcgactg gctagatact tttgaaagta tcgaaaaaaa tggtgcattt tatacgattg
     1321 aatcggagga gaaagataat gaataaggtg gtttatcagc taacaacaga agatgtttcg
     1381 caatttttaa caagcaaaga acacttgaat gaagtaatca gacggattca aaaagacttt
     1441 agttgtatgg aagaaatgaa gtactttatt caaagagtgg aagaacagtt gtatatggaa
     1501 agcgaaacaa gtcaaacgaa ggtcacagtc agccataatc atttagacgt gtataaacgt
     1561 cgtcctgaaa cggtaaaaaa aggaacagag cttctacgac tagaaacaga aaaatacatc
     1621 atgaatgtgt acctagaaag gagtgagctg gtcgaaaact ttgtcagcta cacgatttgt
     1681 aggaaacact attactcaga atcagaatat tattcaattt tcgagagcat ggaattaatg
     1741 gaagaagaaa tggaagagct aaacagcaaa aatgcgacgg tagacgatta ttaccttgta
     1801 tttactaggt ttttgacgtg tgggacggaa tctatcgtgt taatgggaat ggattatgaa
     1861 ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag aagtagataa cccaggcgac
     1921 gactggtaca cgactttaac tgatttttta gacacctaca cttacgacga tacagaagaa
     1981 atccaaaatg aacttgaccg taaacgaatg ccatatcaac gatttgaaag gaaaaaaggt
     2041 aaataaccat ttataccgaa aagtgaggat gtgtgaaata agaacaaaaa accttgaaat
     2101 taagggaatg gttcttattt cgcaccaatc gctccaaccg cccccgccgc ttaggtggtg
     2161 cgaaataagc gggcaaagcc cgataaccta agagaaagaa ggagatccat atgaataaaa
     2221 tcattcctct attgttcgta ctgtttgttg gcgagatact tatgcgtcat gctgcagctg
     2281 tacatttaaa cttcgtagta ttcttaggaa tactggtatt ttgtttgatc ttttggcgat
     2341 ttgtattagc ccttgtgagt ctactattca agatgattgc agccatattt ttcatagggt
     2401 tatttcgatg aaaggagaga gtactaatgc caattgcaat aggaaataaa cggttacccg
     2461 tgacattaga tgaaaagaga caaaaagaat tgcagcaact aaagcagaag tacggaaaaa
     2521 gtgaatctaa gattatgtgt attgcgttag atctattgat cgcgcaagaa aaagcaggat
     2581 ttgatatacc agcactaaaa aagtgacgtc acttttcatt tgaagaaaca aaagtgatag
     2641 cacttaattg ttcctactat ttagaatatt ttctatgtcc tttttattcc attttgtatc
     2701 aataactatg aaatctttaa agtatccaga agagattatt tttagatcaa tttttttctt
     2761 acctgattct aaagattttg ttttcttaaa catattatga tttcctttcg actattttca
     2821 tataaatgtt aacatagttt atggattcat ggaatgttaa tctaatttaa tagaaaagga
     2881 taattaagta tatacgtttt ttcttatgta tgttatacta tgtatataca gaaagaggtg
     2941 tctactatgg aaatgttacc tataaaaaaa tggggaaata gtaatggcct tagattgcca
     3001 aagtatatta tggaatatct tggaattcat acagaagaca aagtgaagat aatacaagaa
     3061 gaagtaaacg gtcagaagcg gctaattatc gaagcggcta atatagaaaa tgaattaaca
     3121 attgaacagt tatttgaaaa ttacactgat gaaagaaatc atgtgacgat tcaaaattta
     3181 ggtgaagcag taggtaatga aaaatggtga gaaaacctag acaaggcgat attctattat
     3241 tgaatacagc acctcgatct ggacacgaac agacaggtaa aagaccttat attgttttaa
     3301 gtcatgatat tattgcggat tatagtaacg ttgttacagt tgctccaatt tctagtacaa
     3361 ctagaaacta tccgctatat gtaaatataa actcagaata tgaaatgaag acaacaggaa
     3421 aagtattatt agatcaacta acgacaattg attatgaagc cagagagtgt atttttttgg
     3481 aaaaagcgca tgatagttta gttgaggagt tattgatgaa agttagaaca gtttttcaaa
     3541 aagtaagcaa ataaaataga aacagcagca tgatcaacta ttcgtgattg accatgctgt
     3601 ctttttttat tctatatacg gtttgtctcg taacattgta gtcttttgca atcttagcga
     3661 tcgcaacacc tttttttaga tcctcaacga tgtttttata aacaagacga cgttggggat
     3721 cttttgcatt ggcactgtat agcttaggac gtcctttata aacaccgttc gcttttgcaa
     3781 tcttaatgcc ttgtgcctga cgtcgtttag attcggtacg ttcttgttca gcgatcatgg
     3841 ccaaaatttg aatgatcaaa tctttaatga atttatccag gagaggattg ccaattgctt
     3901 cggccatgat cggtagactt gtgatgatca gctgcacatt tttcttcttc aagtaattga
     3961 ctgaagcgat gatctcgtcg taatttcttc caagtcgatc aatagcttcc acaataaaaa
     4021 tatcttgatc tcggacgaaa tccagtgctt cttgaaaaac aagtcgatga gtaatcgttg
     4081 cgccagattg tttctcaatg aaaattctct ctgcaccgaa ttttttcatt tcttcaatct
     4141 gccgttgttc attttgatcg atggatgaga cacgtatgta tgcaactctc aaaattttta
     4201 cctactttct gcaactattt aaaaaacacc ctaatcatac acgagaaaac cagatttttc
     4261 aatgtcgcgg tcgtgtctag ttagggtata ccttatatag acaaaaacta atgatggagg
     4321 aaatagtatg tgtcactttt ctttttaaat ggcaatattt tttgtaaata aatgtcaatc
     4381 aagaaaattt aaacgattat gacattaaaa atatatttta tgaattttct agtatataac
     4441 ttgatttatc tgttaagatt tattaagaaa acgttttctt atttttgatt tcgaggtgat
     4501 agacaatgaa aaataagttt attttatctt taggtatttt aactattgga attagtgtaa
     4561 ctggaactac aattcctaca tttgctgata gtacaaatga accaactact gtagtagata
     4621 ataaccaaga attaactcct ttagatagga acggaatagc cattggtaac ccaactaacg
     4681 acttaactgt tactgaaaat tatttaatag tttctccatt acttgctcga gcagcttcta
     4741 aaacgatacg agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc
     4801 ctcaatcacg gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt
     4861 tacaaagtta taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta
     4921 gaaagtaggg attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg
     4981 atattctaag taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt
     5041 tacaggagac acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa
     5101 gaaaaaaaag agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg
     5161 tctagtcgac attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca
     5221 ctttcttttt ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa
     5281 caatatctat gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag
     5341 gagtgatttt tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt
     5401 tacaagaaga cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg
     5461 gggcaaaaag taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata
     5521 cacttgtcgt ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg
     5581 tcaatcgact gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg
     5641 acaaatcaag ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg
     5701 agcgtaattt gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac
     5761 ggtttggtgg tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag
     5821 tggatagcgg tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca
     5881 tttatcgtta tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt
     5941 tgtcatttaa tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat
     6001 tttttgtaag tgtctactga taacttattc tcaaatgttg taaggatttt attcatttgt
     6061 taatat
//
LOCUS       DAZUMO010000121         5717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000121 DAZUMO010000000
VERSION     DAZUMO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.125"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG8ER_002742"
     CDS             196..921
                     /locus_tag="ACG8ER_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051261.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG8ER_002743"
     CDS             911..1420
                     /locus_tag="ACG8ER_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051262.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG8ER_002744"
     CDS             complement(1490..2938)
                     /locus_tag="ACG8ER_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5051263.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG8ER_002745"
     CDS             complement(2938..3654)
                     /locus_tag="ACG8ER_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5051264.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG8ER_002746"
     CDS             complement(3635..3985)
                     /locus_tag="ACG8ER_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5051265.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG8ER_002747"
     CDS             4129..4902
                     /locus_tag="ACG8ER_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5051266.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8ER_002748"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8ER_002748"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5485..>5717)
                     /locus_tag="ACG8ER_002749"
     CDS             complement(5485..>5717)
                     /locus_tag="ACG8ER_002749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051267.1"
                     /translation="FEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLG
                     ISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT         1660 a         1124 c          993 g         1940 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaata
//
LOCUS       DAZUMO010000122         5688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000122 DAZUMO010000000
VERSION     DAZUMO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.126"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..843)
                     /locus_tag="ACG8ER_002750"
     CDS             complement(<1..843)
                     /locus_tag="ACG8ER_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051268.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHH"
     gene            1026..2150
                     /locus_tag="ACG8ER_002751"
     CDS             1026..2150
                     /locus_tag="ACG8ER_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5051269.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            2377..3294
                     /locus_tag="ACG8ER_002752"
     CDS             2377..3294
                     /locus_tag="ACG8ER_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5051270.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            3287..4120
                     /locus_tag="ACG8ER_002753"
     CDS             3287..4120
                     /locus_tag="ACG8ER_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5051271.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            4203..5321
                     /locus_tag="ACG8ER_002754"
     CDS             4203..5321
                     /locus_tag="ACG8ER_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5051272.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            5342..>5688
                     /locus_tag="ACG8ER_002755"
     CDS             5342..>5688
                     /locus_tag="ACG8ER_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS5051273.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYH"
BASE COUNT         1922 a          877 c         1108 g         1781 t
ORIGIN      
        1 atgatggatc gaatgaaatc ctaatatatc tgcaatttct gtataggtta attcattttc
       61 tagcacgaga cgttttgctt tttccatttt tagttcttta aaatagctaa taacactttt
      121 tccattcact tttctaaaat gtttttttaa gctggctgga ctgataaaaa aaatttgggc
      181 taattgttca agcaaaaaat tttctgaaag atgttcttct aaatatcgta tgattggatc
      241 aactaattca tctgaataaa gaatatgctt ttttttagga atgactttct catttgaagg
      301 tattgtcaat aaagcaagtt cctgttgata atgccttggt aaggaagaca attgaatttt
      361 ttgcagacaa taagtaacta ataaggaaag caaatgaatg ctcatctgtt ggcatagtat
      421 gaaaaaagaa ttgatttcct gatcatagga aatagctggc ttactatgtt ttagataatc
      481 aattttgaac tgttcatttt tcagaaaagt aatgatagat gttatttctt gatctataga
      541 aaaagattgt cccgtaatat gtgctaataa ttgctcatca aaataaaact ggataatgat
      601 aaaagtttgt gtttcctttt gagtagaaaa gatttggact tcttgctgtt tatcaatgaa
      661 taaaagggaa ggattatcta gttctataga tttatgtgca aattcagcat ataaattagc
      721 gctttcattt attagtagtc gaaaataggg agcagcagat aaatgaagat gggtatctat
      781 ggaataggtt agtgtttttt tgacaagatt ggaggattgg ttgtttgtta acaaaagaac
      841 cactttcctt tctcttttta tataaaaagc tttctattta taattaatga ctattataac
      901 aaaaatgaaa agccaaaacg acaagtaaaa tattttttta tataaatatt tgtgttacta
      961 attccgttaa tatacataag tgaaagcgtt ttatataaga acttgaaaag aaagaaggga
     1021 tagacatgaa aaaaagatac aaattggtga tagctttgct aaacattacc ctattattca
     1081 gcttaagtgc atgtggaaaa actaacgaag aagtaacagc aaacatcgca gatccaatga
     1141 taaaaaaagc cactacatta gaagtatatg tcgcaaacgg caaactgaca aactatgttg
     1201 aaaatgctgc aaaaatctat aaccgcaaaa ctggagcaaa tattgactta aaaattgtga
     1261 atgtagcttc gggaacagca acagtacaaa tgatcacccc aaaattggta acacacgaag
     1321 atatgccgga tattatctcc atggcagaca cttcagcagc aggtgtgctg gaaaaatttc
     1381 aagatgcttt ttatagtgct gatgaatatg gattttatga caaatatggc gatcagtttt
     1441 atgatcaaaa attgaatatt ttgcgtcaac aaacgagtaa tgaaacgatt attccttggg
     1501 ctagtgattt taccccagca ctttgttatt atcaaccaaa aaaatttgct gaaataggtg
     1561 tagattttga agaaatcagt tcttgggaag aatatatcca ggtggcaaaa gcattaaaag
     1621 aaaaaactgg tagttttgga attgccttgc cagaatctgg ggaccaagaa ttgtttatca
     1681 acatgctagc ccagcagaat caatctttgc ttgatgaaga aggaaatatc cgtctgaata
     1741 ctaaagaagc gaaacatgct gcaaagttaa tcaaagaaat gatcgactca gatattgtac
     1801 atttctacgg tgcacaagat gctgagaaag catttcaaga atctgcaatg tttgttgctg
     1861 gcggctggta tgctacgaat atgtctttga attttcctga tgcatctgac aaatggcgca
     1921 tggctccact gattcctttt tcaaaagaaa atccaggaaa aggtgcagta tcaggaggat
     1981 caagttttta tgtccctaaa gatgccaaaa atccacaagt ggcacaacaa tttcttacat
     2041 ttatgctgac agatgaagaa tgcttagcca acgcactgga gctgggcgtg gctacatcta
     2101 actcattggc ctatcaaaca gaagcagctg agaaaaagtt tgactactaa ggaggacaaa
     2161 aatactatga actattgaat aaaatcaatg aaaatacatc aaatactgtt ttaccagcca
     2221 cttattcaga tgctacgtct tatttgacta ccgctgcata taactattgg caaacaggtg
     2281 attttgaaaa ttcatatcta aaagaagcag aaaattttgc tcataagtac ggcgtaaaat
     2341 taactggtga ttaaaaaata ttaggaggac ttaacgatga tgcaatcttc gttaccaagc
     2401 aagacaataa agaacagcaa gaaaaaagca acagatacgt ctcataaaaa aagtgcttat
     2461 ggattggtca caccttttgt tattttcttt attgtgacga ttttgattcc catcattatc
     2521 tcgtttgctt ttagttttat gaaaattggc tactcatttg aatttgttgg atttgacaat
     2581 tatctagcta tcttccgaga tccattgttt attaaatctt acacgaatgt tttgattatc
     2641 atggcaggat caattccaat aacgatgcta ttagctattt actttgctgt tttattgaat
     2701 tcagcacagt taaaaggaaa aggatttttt agaactgtct attatattcc aaccattaca
     2761 tcaacggtgg ctgtagcttc cgtattcatg acttttttca atccgactgg gttattcaat
     2821 ggatttttga atgcgctagg aattaacggt gtacagtggt taacaaatcc tttttggata
     2881 cgaatatcaa tgatcatcac gatgatttgg cttagtgtag gatataacac tattttattt
     2941 ttagctggtc ttcaagggat ttctaaagag atatacgaaa gtgctgaaat agatggagca
     3001 tcgaaatttc ggcaattctt tttattaacg atccccatgc taaagccaat cattttaatg
     3061 gccacagttt tagccactat caatggcttg ggtaatttca atatcccaaa tatattcttt
     3121 gggacttcca atggaccaga aaattcagct ttggtcgtag gagtcaattt atataaaaca
     3181 agttttgaaa tggttgattt tggaaaagcc tctgctattg cctggacgat ggtgtttgta
     3241 tctgttatat tatcggctat tcaatttaaa ttcggaggtg ataaaaatga ctaaacaaaa
     3301 gatacaaagc agaattttta cgtacgtcat tattgtgttt ggtgccttaa tatttttgat
     3361 gccatattta tatatgattg tagcttcaac gcaaagtaat caagaaattt taagtggcgg
     3421 aatcaatttc aagtttgggg atatgctatt aaaaaactgg caagccatca tggcgagata
     3481 taattatccc ttgattattt ggaattcgat tgttattgct gtaatcagta cagtttcagc
     3541 tacagccagt tctacaatgg cgggttatgc attagcaaag tatcgtttta tggggaataa
     3601 actgttgttc aatacagtta tgctatctag gatggtgcct ttttttacca cattgattcc
     3661 tttattttac attatgagtc gtttaggatt atcgaatact tatgcaggtg tgatcatccc
     3721 ttcgattgcc agtacaactt cagtgtttat gatgcgacaa tatagcagtc aatttcctac
     3781 agcactgatg gaggctgcca gaatcgatgg tgccaatgag tgggtgattt ttacgaaaat
     3841 cgctgtaccg attttatcac ctacgattat tactagtggt ttacttatct ttatgggaag
     3901 ttggaatcaa tatctatttc cattagtcat gctttcagat aaagataaat atacggttcc
     3961 cttaattatc aaaaatatga cggtttctag tacaggtgag ccaattaatt atggcgcaat
     4021 tatgctaatt ttagcaactt cagtgatccc aatggtcttg atttatgtgt gggcgcagtc
     4081 taaatttaag gagaatgaca ttggagctgc aaataagtaa aaaaaataac gaatgttaac
     4141 catctagttc gttctaggag caactccaca ttcataaaaa ttaaaagaat ggcgggatat
     4201 ccatgaatga acagacgaag gaatggtcaa gaaaaatttt gaatgtagct tctgaaaaga
     4261 tagatgcgga attagacaga atggggaata aaattccata tatttcttac aacggaaaat
     4321 atacagacgt aataaaggac agaggaatcg actggtggac caatggtttt tggagtggta
     4381 ttttatggca gctctttcac aagacaaaac gagaaaagtt caaagaggca gcaatacaac
     4441 aagaacaatt attagaatct gccctgtacc atttttcaga cgttcatcat gatgtaggtt
     4501 ttttattctt accatcggct attgcacatt atcaacagtc tcaaaacaaa catagtcgac
     4561 aactagggtt acatgcagct acggtacttg ctggacgcta taatatttca gggaactatt
     4621 tagtagcatg gaatgaccat aaacctggtt ggatgattat tgatagtatg atgaatttac
     4681 cattactgta ttgggcttcg agggaaactg gtgatccgag gttctctaaa attgctcggc
     4741 agcatgcaga tacaacagct aagtatctag tgaggaaaga tggatcaacc gggcatatcg
     4801 cagaatttga tgcagaatca gggaaatata ttcggttact tggcgggcaa ggatacaatg
     4861 agaattcttg ttggagcaga ggaaatgcat gggctttata tggattcgcg ttaagttatc
     4921 gttacacaaa aaatccgtac tatctagcta tagcagaaaa gatagcagta aattttattc
     4981 agaaaatcga aaaaactggt tatattcctc tcattgattt tgctgtaaaa gattcgaata
     5041 ttgataccag tgctggagta tgcgctgcat gcggtcttct ggaattagca ggatatgttt
     5101 cctctgtacg atcaaaaatg tatcatgaag ctgctatcaa tatactccaa aagacagaaa
     5161 agcactatgc aaattggaat atggatgaag atgggatcat tggaggcgga gcagaagcat
     5221 accatagaga actgacttat aatgtgccac tgatctattc tgattatttt tttattgaag
     5281 gcttacttcg attagaacag tcggaattag ctatttggta gtaaaaagag gagaaaatac
     5341 aatgaaacat ccagtaccac tattaaggac cagacaggat tactttgact tatttaaaaa
     5401 gataaatcaa ccactccagc cgttttatcg agcaagtggc gcacaaattg attatataca
     5461 tcaaggggta ggatatggaa ataaaattgc aggaatggaa ggattctctc gggttttatg
     5521 gggagcgggg cccgcaattg accaattaga tgaacgatgg ttgaaattaa ttctatcagg
     5581 aataaaagct ggaacagatc cttcccatcc cgattattgg ggagaaatac atgaaagaga
     5641 ccagcgtatg gtagaaatgc ccgcaatgtt attagcgtta tatcatca
//
LOCUS       DAZUMO010000123         5663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000123 DAZUMO010000000
VERSION     DAZUMO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.127"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..1168
                     /locus_tag="ACG8ER_002756"
     CDS             176..1168
                     /locus_tag="ACG8ER_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5051274.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1228..2649
                     /locus_tag="ACG8ER_002757"
     CDS             1228..2649
                     /locus_tag="ACG8ER_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS5051275.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2642..4141
                     /locus_tag="ACG8ER_002758"
     CDS             2642..4141
                     /locus_tag="ACG8ER_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS5051276.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4180..5574
                     /gene="uxaC"
                     /locus_tag="ACG8ER_002759"
     CDS             4180..5574
                     /gene="uxaC"
                     /locus_tag="ACG8ER_002759"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5051277.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1858 a         1051 c         1163 g         1591 t
ORIGIN      
        1 ctcatctaat taaatgagca ttccccgctt tcaagaatag cataattaaa aataaaaatc
       61 caagtaattt ttaacgtgtg caatcttttc atatcaaaga tctattgact tatcgtagtt
      121 atcgtaatat gatgaatgcg ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt
      181 aacgatcaaa gatatcgcta aagcagctgg cgtttctcat acgacagttt ctcgtgcttt
      241 gaataacagc agtttaataa aaaatgaaac aaaacaaaaa atacgacaac tagctaagga
      301 aatgaattat tcgccaaact atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat
      361 cggactattt ttctcaagtt tgcataaagg aacttcatca aatttcctag cagattccat
      421 tgcaggcatc cattcagtat tagacacaaa ttacagttta tctgttgaaa gtatcgatgt
      481 tgttgattta acagaaatca acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag
      541 tgatgatgat cagcctttca tcgactatct caaagaaaaa gaatttcctt tcgttgttgt
      601 caatcgtcac atcaatgatc cagcggttat caacgtcact gccgatgatg cggccggtgt
      661 tatgcaggca gtagattacg gtattggatg cggacatacg aagatcgctt atattggcgg
      721 aaagaaaaat tttcgttcga caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa
      781 agcgagcatc cctgtcaatc tagcctattt tttaacagga gattacagct tagaaagcgg
      841 cctccaaaac atggaatacc tattatcact ttctgacctg cctactctgg ttttttgcgg
      901 caacgacgac atagcgattg gtgcattgag agctgtacat ttagctggcc tccgtgttcc
      961 ccagaacatt tctctgatcg ggttcgatga ttctccagtc gtctcctatc tcaatccgcc
     1021 gcttaccacc gtacgtaagc ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact
     1081 agatttgatc gaaggcagga cgatcgaaca atctaaaatt gagctctcca ccttgttgat
     1141 ccttcgtgag tcgatcaatt acttgtaaac tacaagtaat tttatataaa aatgttaacg
     1201 tgtgcataac acagaaagga agtttatatg aaaaaactga caaaagaatt agtgaaaaaa
     1261 aacgaagcac ctgtaaaagt tcttcagttt ggtgaaggca attttatgcg tggatttatt
     1321 gattggcaga ttcagcagct gaataaccaa caattgttta aaggtaatgt tgcaatcatt
     1381 caacctttag aacaaggtct tggaaatatg atgaaccagc aagataattt atatacggtc
     1441 attttacaag gactcagcaa tggcgaaatc gttgatactt ccgagattat caccgtagta
     1501 gaacaaatga tcaatcctta tgatgaatgg gatgcttatc tatctctagc agaaaatgac
     1561 gacttggcct atatcgtctc caatacaaca gaggcaggaa tccaattcgt ggagactgat
     1621 aatttaacag atactccccc acagagcttc ccgggcaaac tgacagcctt tttataccga
     1681 cgtttcaaat taaacaaagc tggctttacc atcattcctt gcgaactgat tgacagaaac
     1741 ggcgaacgtc taaaagaaat cattttacgc tacgcagaaa aatgggattt agaacctgct
     1801 tttgttgagt ggttagaaaa agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc
     1861 ccaggttatc ctcgcgaaga tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa
     1921 ttgatggtca aagcagaacc attcatgctt tgggtgatcg aagggcctaa agaattggag
     1981 aatcaactac cattcaaaca ggctggcttg aatgtcatca tcacagatga tatgactcct
     2041 tatcgtgaac gtaaggtgca tttgcttaat ggaccgcata cagcgatggt ccctcttgct
     2101 ttgttggcgg aattggaaac tgtagaagaa gtaatgaaag atcctttatt ctccgcatat
     2161 gttgatcagt tattcaatct agaattgatt cctatgcttt ctttacctaa agatgaatta
     2221 gccatttatg ctgaccaaat caaagaacgt ttcttgaatc cttttgccca tcacaaatta
     2281 gaagcgattt cattaaatag tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag
     2341 aagtatatcg aagaacaaaa tcaggtacca ccgttaatca ctgtttcact agctgcgcta
     2401 cttcttatgt atcgaggcga tcaagtaaag ccacatgatg atgaaaagac gataagcgaa
     2461 tttactgatg cttggtctga taatggaaca gctattcctc gattattgca aaacgctgcg
     2521 ctttggggag aagatttgtc tcaaataccg aatgtgacag atacagttca agaaatagct
     2581 gcaaagatcg atcaaaaagg tgtccggtca gtattacaag aaatgaaagg aacgatggtc
     2641 catgcttgaa accctgcaac caacaatgaa attaaatcga cgagacagtg tggtcgtcgc
     2701 attaacccct attccaaaac aaacacaact gacgattgat cagcaaacga tcaccactct
     2761 tgaagatatc ccgcaaggtc ataagattgc attgggtgat ttgaaagctg gagacaacgt
     2821 gatcaaatat ggctatccaa tcggtcatgt gacgacagac gtaactgccg gtcaatggct
     2881 gcacactcat aatgtaaaaa ccaatctttc tggtgaattg gattatcgat atgaaaaaga
     2941 tgtccatcca agtcattacc tttttgagaa ccgaacattt caaggttact tgcgaaaaaa
     3001 tggaaaagta gggattcgta atgatttgtt tttagtacct accgttgggt gtgtgaatgg
     3061 cattgctgaa ctgatcgtca aacagttcaa ggaaaaacat ccagatctgg gacagtttga
     3121 ccatattacg attttaaagc atccttatgg ctgttcgcag cttggaaaag atcatcaaaa
     3181 tactcgagaa attttagcgg acgcagtaaa tcatcccaat gccggaggag ttttagtatt
     3241 tggattaggt tgtgagaaca atacagttcc tgaattcaaa aaaattttag gtgaatatga
     3301 tgaagaacga gtgaaatttc tagtggctca agatgtttac gatgagatcg aacaaggtgt
     3361 ggctctatta gaagagcttt taacagcggc ggaaaatgac cagcgcatat cagtcccgct
     3421 ctctaagttg aatgtcggtt tgaaatgcgg cggttctgat ggtctatcgg gaataactgc
     3481 taatccttta cttggtgcat tttctgacta cttgattgct caaggaggca gcacgatctt
     3541 aacagaagtg ccagaaatgt ttggcgcaga acaagtattg atggcacgag cggaaaatga
     3601 agaagttttt gactcaatcg ttgacttgat caatgacttc aaacagtatt tcttgtctta
     3661 cggggaacca gtttacgaaa acccatctcc tggtaataaa gcaggtggga tcactacatt
     3721 agaagataag tctttaggct gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct
     3781 acaatatggc gaaaaaatca gaaaaccagg gcttagttta ttgcaagctc ccgggaatga
     3841 tcttgtagct gcctctgctc ttgcttcatc cgattgccag ttggtcttgt ttaccacagg
     3901 aagagggact ccgtttggct catatgtccc aacggtcaag gtatctacga atacaactct
     3961 ttttgaacgc aaaggtcatt ggatggattt taatgcaggc gtcttattag aacaaccaat
     4021 ggaagtaatc ttagaatcgt ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac
     4081 gaaaaatgaa caaaatgacg ttcgagaaat tgccattttt aaaaacggag taacactttg
     4141 attttcataa aacagtataa aaagaaagaa ggaagattta tgtttttatc tgatgatttt
     4201 ttattaaaag acgattgggc gaaaaagctt taccattcct acgcaaaaaa catgcccatt
     4261 attgattatc actgtcactt agatccaaaa gaaatttacg aaaacaacaa tttcagcaat
     4321 ctgacagaag cttggttagg aggcgatcat tacaaatggc gcttgatgcg agcttgcggc
     4381 gtgcctgaag aaaaaatcac aggaaatgct tctgattttg aaaaattttt agcttggtgt
     4441 caaaccgtac caaaaattat cggaaatccg ctttacagtt ggtcacatct tgaattgaag
     4501 cgtttcttcg gtattgactt gttgataaat gaagaaaatg cagagaaaat ttggacacaa
     4561 gcaaatgaaa ttttggcagc tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat
     4621 gtagaagtga tttgtacaac agatgatccc gcagacgact tgcactatca tcagttgctt
     4681 gctcatgaag aaccagattt gaaggttcta ccttcattcc gtccagataa agcactcaat
     4741 attgaaaaag aaggatttaa tgcatgggtc acactactgg aagaagcagc tgaccaaacg
     4801 atcaccacct atcaagaact gatagaagtc ttagggcagc ggatagaata cttccatcaa
     4861 catggatgtc gtgtttccga ccacgcttta gacatcttac gctataaaga agcggatgaa
     4921 acagtattag aagagatttt tcaaaaagca cgagctaaca aactattatc ttcagatgaa
     4981 atcgatgctt atcttacaga aacactgata catttgaccc acttctatca ttctcataat
     5041 tggacgatgc agctgcacat ccatgcttat cgtaactgta atacacaaat gttggaaaaa
     5101 ttagggcccg ataccggcta tgatggaatg aatgatcttt ctttgactat tcctctgcaa
     5161 aaattattga accgtgcaga agaaacggat cagttgccaa agacgatcct ttattcattg
     5221 aatccgaatg actatcctgc gattttagct ttgatgggct gtttccaaaa agaacagaat
     5281 ggaaaactgc agttaggttc tggatggtgg tacaacgata ctcgagctgg tatgcgcgat
     5341 caattgacac aatttgctga tggtagtgct ttggggaatt ttgtcggcat gctgacagat
     5401 tcaagaagtt tcctgtccta cactagacat gaatatttcc gtcgtgttct ttgtgaattt
     5461 ttaggcgaaa tggtcatgcg aggagaagca cctgaagatg aacaactttt aggaagtcta
     5521 gttcaagcga ttagttatac aaatgctaaa aattactttg gcttttttgc ctaattgatt
     5581 aatagtaaca gtaacgaatc aaaaacagga tagtgaaata acggaatccg tttatccacg
     5641 ttttagagaa gaagacggac caa
//
LOCUS       DAZUMO010000124         5377 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000124 DAZUMO010000000
VERSION     DAZUMO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.128"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..1111
                     /locus_tag="ACG8ER_002760"
     CDS             236..1111
                     /locus_tag="ACG8ER_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5051278.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1215..1979
                     /locus_tag="ACG8ER_002761"
     CDS             1215..1979
                     /locus_tag="ACG8ER_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS5051279.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1966..3408
                     /locus_tag="ACG8ER_002762"
     CDS             1966..3408
                     /locus_tag="ACG8ER_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5051280.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3419..4749
                     /locus_tag="ACG8ER_002763"
                     /pseudo
     CDS             3419..4749
                     /locus_tag="ACG8ER_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4762..5220
                     /locus_tag="ACG8ER_002764"
     CDS             4762..5220
                     /locus_tag="ACG8ER_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5051281.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1819 a          778 c         1121 g         1659 t
ORIGIN      
        1 tttcttatag agagttgcta tatttttttt gtttttatgt aattacctcc aaatcaattc
       61 gatacaagta ttatcataaa acgagtaaaa aagatattga taacgctttt attatgtgtt
      121 aaactatttt tgtaaattat gtagggtgct actattcaat taggcacgac ctaagaaagg
      181 gttctttctt aggtcgtttt ttgtctttaa aaattgtttg gagacaaaaa tatgtatgaa
      241 ggagtctgca agttgttttg ttttatcata tagtggaggt gaattaacgg tgtatgtgat
      301 gaaaaagctg aatcataatg ctgtattgat cgatgacgat ggaatagaaa aaattgcgat
      361 ggggaaaggg attggctttt ctgctaaagt gagtaagcca tttgatgttg aggatgctga
      421 caaattattt gtattagatt ctaaagaaaa gatcaaacga ttcagtgaga tagtctcgca
      481 gattccaatc gaatttatgg attttgcgga agaagtgatt gaactgataa cagctaggat
      541 ctcctctcca ttagattcaa atatctatat tacattgaca gatcatattt attttgctat
      601 tcaacgaaaa agagaagata gagatgtcac agcaattatg ttgccagaaa tgagattcct
      661 ttatccaaat gaatatgata tagcaataga agtggtcgag cgaatcaatc atcgatatca
      721 aaccgagtta gggtcaaatg aagtaggatt catcactatg catatcatta atgctgagct
      781 aggagagcct aatagtttaa agagcttaaa aatagtagaa atgacgacca cgattttgtc
      841 ccatctagaa aaatcctatt caaacgttta tgataaagaa tcactgactt atcatcgctt
      901 aatggttcat atcaaattcc tggttaaacg cttgatttat caggaagaaa cgccagtaga
      961 aaatattggt ttttttactg aaacatttaa acatagcaaa gcgtattgta cagcactaaa
     1021 gattaaagat atcatcgaac agcaatttca acttgccatt caagaaaatg aaatcatcta
     1081 tttggcaatt catctagcac gaatctatta aaataagggc gtaactatct ataggcgcaa
     1141 ccaaatgaca catagtaaat ggactatgat tatttggttg cgcctatttt agtagaaaaa
     1201 agaaggagaa tgaaatggga aaagtgattt ttaattcgga tgattttggc tatagccatg
     1261 gtgtgaatta tgggattatg gatgcttatc agcgaggaat tttgacatca actacattga
     1321 tggcaaatat gccgggtttc gagcacgctg tgaaactaag aaaggaaatg cctaggctag
     1381 gtgtaggagt ccatttaaca ttgacgtgtg gcaaaccact tctcaaaact gtcgatactc
     1441 ttatcgaaag cggagagttc agatccttat cttattatca gcatccattt aacattgaca
     1501 aagatcaact gtatcaggaa tggaatgctc agattcaaaa aatttatcat gcggggatca
     1561 taccaactca tttagacagt catcatcata ctcatacttt ggatgaaaac caggaggtag
     1621 ttgttacatt agcaaaaaaa tatgatttgc cggttcggag caactttgaa cgaaaagatg
     1681 aagttcgtca tgtcgattat tttgagcctt gtttcgatgt cgtaggagaa actgatgaaa
     1741 aaaaacgaca aatcaatacg agtgtggaag aatatttggc aagtttactc atgatattga
     1801 gagaagataa aacgatcgaa gtcatgtgtc acgcagcgta tttggatcag gaattgttaa
     1861 atggttcaag ttttgtgtat cctcgtactc accaagtcga atttttaatt cattcagcat
     1921 ttgctcaaaa agtgaaaaat gatcctttga ttgagttagt aacatatgag gatatttaat
     1981 agaggaggaa taaaaatgaa tgttaaatca aatgtgcaga agcttggaag agcgatgcta
     2041 cttccagttg cagcgatgcc attagcggga ttgatcatgc gtttatcagc tgatgatatg
     2101 ctgaacatcc cagtaattgg tgcagctgga aatgccgttt ttggaaactt ggatatctta
     2161 tttgctatag gagttgcaat cggttttgtc aaaaataagg acaaaggaat acctgcactt
     2221 actggttttt tagcgattgc taccttgaaa aaaggcttag aaatcatgga cccagaagtg
     2281 aatatgggga tttttggtgg aattatttcc ggtttagtag cggcatggac gtataatcgc
     2341 tttaaagaac aaaaattacc agtagttttt tcttattttg cgggcgaaaa attcccattg
     2401 actatggtga tgattctaca aacaatcgca gctatttttt tgggctttat ctggccgctc
     2461 atccaaattg ggattgataa ttttgcaaaa ctgctcgttg atatgggggc attaggtgtg
     2521 gggatattta tgtttttgaa tcgtttgctg atcccctttg gactgcacca tgtgttgaat
     2581 acgtatattt attacgattt aggaagctat acatctccta gcaaggaagt tttcagagga
     2641 gagatgacac gctttatcaa cggagatcct caggctggac tgtttttatc aggttttttt
     2701 gtagtaatga tgtttggtat accagctata tgtctagcta tctataaagc agcttataaa
     2761 gaaaataaag agatggtcaa aggaatcatg ggaagcggtg caggaacttc atttattgca
     2821 aatatcactg agcctgttga atttagtttc atgtttcttt ctcctatgtt gtacgtgatc
     2881 catgcacttt ttgccggttt agcagggatt gtctgttact tattcaatat acgaattggt
     2941 tttacattcg gagcatgtat tgttgattat ctcatcaatt ttcgaatcgc taccaatgct
     3001 attttgattc ttccaatcgg aattttcttt tttgccttgt attacgttac tttttattat
     3061 ttgatcaata agagaaacat tcagacatta ggacgtgaag ctaaggcaga gtttggcaat
     3121 gaagtaacct tagaagaaac agagttaggg ttagcaagta aaaattatga atatatggca
     3181 acaaaaatgc tacaagcgtt tggaggaaaa gccaatatcc tggatgtcta tagttgtaat
     3241 acacgcctac gggtagaggt agtggatccc accatggtcg aggaacagcg aatcaagcaa
     3301 ttagggataa gcgggatcat taaaccaact gaaaaaaatt atcaaattat catcggctta
     3361 gaagtgactt atgttatggc agaattcaat aaattattag aagaatagaa aggaaaagat
     3421 gatgatgaaa aaacagatta tcactatagc tggaggcggg agtacgtata caccaggaat
     3481 cgttcaagcc attttgaaca atgaaaaacg gttgccatta tctgaaatac gtttatatga
     3541 tattgatgaa gaaagaaata tggatatgta cttgattgtt aaatttatgc tgaaaaaaaa
     3601 ggtttttcaa atattcgcat tcgtgcaacg gatgatccaa agcttgcttt tacaggatgc
     3661 gattttgttt tctcacaaat tcgtgttgga ggattggaga tgcgagaaaa ggacgaaaag
     3721 attccactta aacatggatt agtaggacag gaaacgtgtg gactcggagg atttgcttat
     3781 gggatgcgct caatcaaagg tttattggaa attgtcgatc atatacaaga ctatgccccc
     3841 aaagcatgga ttttgaatta tacaaatcca gagtcgatcg tttctgaagc tgttcgtcgt
     3901 aaatatccga atgtaaaaat gattaatgcc tgtgatatga ctatttcaat tgaagaaacg
     3961 attgcagtga attacggtta tgatcgtaag aattggatcg ttacgtatta tggtttaaac
     4021 cactttggct ggtatacatc gatttatgat aaagaattgc aacgagaaat tatgccagag
     4081 attattgaaa aactaataac aaaagaaatg caagtcgccg attttaatat aggagacaaa
     4141 acttggcaaa aaacctttca aatgatgtca gtcatcacga aaaattttcc aagtaatatc
     4201 ccgaataatt acttagagta ttacttatat cctgatatgg tcgttgagca tactgacaaa
     4261 gagtatacac gtgctaatat ggtcatggat ggacgagaga aaaataccaa agaaatggct
     4321 gataaaatac gccgcggtat tgtggaagaa gtattgaact ttaattttgg ggagcatgga
     4381 caatatatcg tcgatatagc tatttccttg ttgaatgatg atcgtcgtcg ctttatgcta
     4441 atcgttccta atcaaggggc gattccgaat ctacggtcag atgcagttgt tgaaataccg
     4501 gcatatgttg gtgcaactgg tgtggagaca attacactaa gaaaaccaat cagtgatttt
     4561 cataaaggat tgatggaggc acaagttgca gcagagaagt tactagtaga tgcttacttt
     4621 gaaggttctt atcaaaaagc tttacaagca tttacattga atcaaaccgt accaaatgcc
     4681 agagtcgcaa aaaaaatact agatgaaatg atcaaagtaa acaaaaaata ttggccagca
     4741 ttaaagtaga ggtgataaaa agtgcatttc tttaaaacaa gaaacattct aaaagctgta
     4801 gctagtggtc aaattattcc attaacagca gtaaaagacg aggtcttttc ttcaaaaatg
     4861 ataggagttg gatacgctat aacgaatcat gatggatata tccatgcgcc agttaaagga
     4921 ataatcgata atatatttcc tacccgacat gccataacga tcgaaagtga atctggcaga
     4981 aaaatactga tccatatggg tttagataca gttgaactcg aaggaagtcc gtttcagata
     5041 cgggttgaaa gtaaccagga ggtaaaagaa gggacactgt tagctattat agatttgaag
     5101 aagttggagc aagcacaaaa agatacggtg atcattgtag tttctccagg catttttgaa
     5161 ggaaatttat taaaacaaaa ccaagaagta aagattaaag atagttgttt tgaattttag
     5221 tgtacgatga aaaaagttaa atagggtgat atttatgggt gaatttacaa aatttttttg
     5281 aaatgataag tttcaatcac tagatttaat cattttgatt tttgttaatt atcgaatttt
     5341 tttgttgctc ctatgattta gaaaacttat tttcagg
//
LOCUS       DAZUMO010000125         5343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000125 DAZUMO010000000
VERSION     DAZUMO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.129"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG8ER_002765"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG8ER_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG8ER_002766"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG8ER_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="ACG8ER_002767"
     CDS             2092..3132
                     /locus_tag="ACG8ER_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5051282.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /gene="traE"
                     /locus_tag="ACG8ER_002768"
     CDS             3956..4288
                     /gene="traE"
                     /locus_tag="ACG8ER_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS5051283.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="ACG8ER_002769"
     CDS             4907..5248
                     /locus_tag="ACG8ER_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS5051284.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1944 a          728 c          899 g         1772 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta taacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 ttt
//
LOCUS       DAZUMO010000126         5259 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000126 DAZUMO010000000
VERSION     DAZUMO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.130"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..1963)
                     /gene="uidA"
                     /locus_tag="ACG8ER_002770"
     CDS             complement(155..1963)
                     /gene="uidA"
                     /locus_tag="ACG8ER_002770"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS5051285.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(1986..3752)
                     /locus_tag="ACG8ER_002771"
     CDS             complement(1986..3752)
                     /locus_tag="ACG8ER_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS5051286.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(3765..4559)
                     /locus_tag="ACG8ER_002772"
     CDS             complement(3765..4559)
                     /locus_tag="ACG8ER_002772"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5051287.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(4569..>5259)
                     /locus_tag="ACG8ER_002773"
     CDS             complement(4569..>5259)
                     /locus_tag="ACG8ER_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5051288.1"
                     /translation="SLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQ
                     GIVLVNFINPILGLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYV
                     FLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAG
                     GVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVL
                     ENCELLKQKHK"
BASE COUNT         1709 a         1045 c          624 g         1881 t
ORIGIN      
        1 atacaacaat cagaaagccc ctttttttcg aaaaaactag aaaaaaaaca taaaaatatt
       61 tatcattctt taaacagaaa atctttctat aaaaaacaaa aaaaaacgat taggctaggc
      121 taatcgtttt ttgaacattc ttaaaaaatc caaattattt tttataactg aaattgggta
      181 ttgatgacca tcgtttctgt agatagtaag caatcatttt aggttttcta tctctagtaa
      241 agattccttt cttatttcct tgcactctta aaatcccttg acttgttgca aaatctgcaa
      301 aattccacac ttgttcacca acaaaatttt cacatgtatc tattacttcg tgatttgctt
      361 cgtaataagc cacttgatac tcttctgtaa acattgttgg tactgtatca tgaaatccag
      421 ctactgtatc tgctccatat tctgtaaaca ttattggcgt atcaggacat ctttccatcc
      481 atccatctag ctcttttttc aacagatatt ttgcatgtgc cagatcccct cccgaaacat
      541 accaaccata ataacgattt aaacataaaa catcgacaat atctcctacc ttacaaacat
      601 ctggcgttga ttttagataa gtaacgattg ttacaggtct tttctgcgga tcaagttctt
      661 tagttaattc aactaaaggt tcaaaatatt ctttagcacc ttcggaatcc gaatctggtt
      721 catttgcaat agaccacatt acaacacttg ggtggttctt gtcgcgttct atcaattcct
      781 tgatcaccgt tttatgagaa ttttctgttc gtatctcttt ccacgtatta tgtttttcca
      841 cattatcatc taataaagtg gccatgaaat ttaaatgtaa ccctactgca ggtacttcat
      901 caataactac aataccttct cgatctgcta atcgcataat ttcttctgag taaggataat
      961 gtgatgttct aaaagaatta gcacctatcc atttcattaa attaaaatct ttaatattta
     1021 cagcctcatt caaaccacgt ccattaacat acgaatcctc atgcttacca aatcctttaa
     1081 aataaaaagg tttattatta atcaaaaatt tgccattggt aacctgaaca ctgcgaatac
     1141 caaattcttc tgtataggta tcgatcactt tattatcatc aattaaacta attttcatac
     1201 tatataaata agcgtctaga ggttcccaaa aaattggatt cttaagctga atcattcctt
     1261 tctttccttc agcttcagct acaagttgag cctcttcgtc atatatcgct attaagattt
     1321 ttccggtcca ttctgtatcg atattgaatt ctacacttgc atccaaatca gttattcttg
     1381 tattaatagt aatatcactt atatagtctt tcggagttgt atataactta acaggtctat
     1441 gaatacctgc ataattgaag aaatcaaaat taactaagtt ttcctttttt atatccccat
     1501 tttcctgtac tgtttccttc ataatcccca caggtaaagt actatcgtca ataatattgt
     1561 ttacagcaac tgtaattcga ttattcccgt cttttacaaa ccgattaatt tcagactcaa
     1621 atggtgtaaa cccacctttg tgttgagtaa taaactttcc attcaaaaaa acttttgctt
     1681 catgtgttgc agatccaaaa cgaagaacta atctttcttc taataatctt ttaggaagag
     1741 tcacttcttt ttcataccac acccatccaa catgatttct aatgttctct gtttcaaata
     1801 aatcattata agaagaggga actgaaatcg gataactctc tttttcattt attggttcta
     1861 aagcaagttc ttcctgtaag tcatcatttg tatctaattt aaatctccag agaccgctta
     1921 agtcaattac ttgcctcgtc tctgtcataa tgggatacaa catataaacc actcctatct
     1981 aaatattaaa taaatgtatc ctttaaacca caataagcat ctattggact tactccctta
     2041 aattcagaaa gacccattag tttgtccacc aatatttcta aagtttcttg ttttccatca
     2101 taagtattaa tataagtctt tacttgaggt acatcagcca aatgaaacgg acactgtaca
     2161 gatacaaaaa ttgttggaat ttcatgaatg taaaacggta tatccggagt tcctttactt
     2221 gctggccaga caattcgttg aactgtgcct ggattaacat tagctatatt aatgatcaag
     2281 tcatattttt tagttaaatt tttaattggt ctcttagcag catatattcc agctatattt
     2341 ttaatttttt cttcttgcga taatcctttc aattcttctt caggtgaaat gtatttttca
     2401 acttcaaatc cttttgctga caaaatctgt ttcaataaat caatggcttg aggtctattt
     2461 ccagcaacta cttcaccaaa cccacctttt accccatgta catctactag taaaatcctt
     2521 ttatattttt ctggtacaat aggccaaata tcttgtttat ctttaactaa tgtaattgct
     2581 tggtcagcca cctctttaaa aattgattta ttttcaatta aaccaatttt ttccagagct
     2641 tcttgcttag gtaataaaat ttgttctttt gcttttttgt gtaatcccaa tttagctttt
     2701 aaccctaaaa ttctttttaa ggcatctaat aaccgatcat cagtgataat tcctttacga
     2761 tagccttcca tcatataatt aaagtcttca tctggatcat taaaaaatag aaatagatca
     2821 catccagctg caatagaagt gggcaataat tctgatcgtt tcattgcccc agtcattcct
     2881 aacatatgac tagcatcagt aactactaat ccattaaatc cgagttttcc tctaagtaag
     2941 tcagtgatta attcttttga taaagttgca ggaagtaaat cttcatcttt cagatcagga
     3001 ttaaaatgtt ttgaataagc tggcatatgg atatgacctg ccataattga aggtaatcca
     3061 gcttctatta agcctgagta tacttttcca aaagaattat cccattcttc cgtacctaaa
     3121 ctattaatac taaaagatag atgctgatct cgttcgtcaa taccgtcacc agggaaatgt
     3181 ttagctgcag gcaaaattcc actctcctga atacctttca tataagctaa actaagttct
     3241 aaaactttat cagggttttg tccaaaagat cgactagaaa tgataggatt tctccaattt
     3301 ctattaatat ctacaatagg agcaaaactc cagttacatc caatagccga tgcttcaatc
     3361 ccagaaactc tccctaattc ataagcatat ttcgcatcat ttgttgcacc aatttttact
     3421 tctacaccaa cttcagtacc atctgtacaa gctccgttac cacctgcttc cgtattagca
     3481 gcaattaata gaggaatttt actttctgtt tgtaagatat agttttgatc atatacctca
     3541 tcagcagttc ctggattata tcgtacagct ccaatatggt actgattgac catattactc
     3601 aagtaatttt tatcacgact agatcccata ttaacaaaca attgacctat tttttcttcc
     3661 aaagtcatag aatcaatcgt ctcttcaatc caagaaatag catcttcatc taaattatat
     3721 ggtttttttc gtaaatctac ttttatagtc attttctttc tcctctactt attagtaaga
     3781 tttttccaaa aagtatctat atatttttgt tcaatatcta cataatctcc cgataaacat
     3841 ttaaataaaa catcagttct taagttttcc catgattctt tttctttatt gaccaaaata
     3901 ggataaaaga aaactttatt tttatttgca gcttctagat caccaggaga atcaccaatc
     3961 attaatatct tatccaaatc acaaccattt tcaattacac ttcgaataat atcttctttt
     4021 tttcctttat cttgacaaca cagttcatca actaatgata ataattcatg tctttcccat
     4081 tcttcttgaa cagcttcctt attagctgag cttacaataa ttatcttccc atattttttc
     4141 agagctttta tagtatctct agcacttaaa aagggtttat ctaatccttt tgcttgacta
     4201 atctgttgat tgacttcttc cgaccatttt aaagctttta ataaatcctc tgactgagaa
     4261 tattctattt ccctcttcaa agatggatta gacaattctt ttgttagaga tacccagtgc
     4321 tttaatcgat taatcgatgt tacatcattt ttttctaatg ttttaactaa acctaaaaat
     4381 ctgtttattc cacgagtctc tgaatataaa ttaatattat tccaatcctg taggaatttt
     4441 tctctatgat gaacatcaaa aatctctgca gcaattggtc cgaaaaagag attgtgttta
     4501 taagtcatcg tatccataac acagccatct gaatcaatac aaaatatcca attattcata
     4561 tttctctcct atttatgttt ttgttttaaa agctcgcaat tttctaagac tttatcttta
     4621 gataatggat aaaggaaaaa taaaattaat cctacaatca gaaaacaaat tgatggaatt
     4681 aatgttgaag cgttatataa cttttctaaa acatctcccg tttgtacatt tgctaaagag
     4741 tcatagccta taatagaaag agtccaacct ccgacgcctc cagctagagc ttgacctatt
     4801 tttctagcaa aagaatatac cgcatatacg gtcccatctt ctctttgatc agactttact
     4861 tcttgatcat caataacatc agtaatattt gcccaaataa cagtattaaa cacacccaaa
     4921 cccatatagc caaccgaact aagaattaag aatacataca tattactagc ttttatcaaa
     4981 aataagatca aatatactat tgctgaaaag aacattccta ctgatgccag ttcttttttt
     5041 ccatattttt tacttaaaaa caaagttaaa ggcgaaacaa ctattaaacc caaaatagga
     5101 ttgataaaat tcactaaaac tattccctgt gcatcattat agtaatcagg aaatacataa
     5161 ttattcatac tcataattaa taattgagca aggagtaaga aaatagccgc accaattata
     5221 gaaagtaatg ccctattttt aactaaccct tttaaagat
//
LOCUS       DAZUMO010000127         5247 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000127 DAZUMO010000000
VERSION     DAZUMO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.131"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(335..2101)
                     /locus_tag="ACG8ER_002774"
     CDS             complement(335..2101)
                     /locus_tag="ACG8ER_002774"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5051289.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(2061..3815)
                     /locus_tag="ACG8ER_002775"
     CDS             complement(2061..3815)
                     /locus_tag="ACG8ER_002775"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5051290.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3925..4494)
                     /locus_tag="ACG8ER_002776"
     CDS             complement(3925..4494)
                     /locus_tag="ACG8ER_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS5051291.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4512..>5247)
                     /locus_tag="ACG8ER_002777"
     CDS             complement(4512..>5247)
                     /locus_tag="ACG8ER_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5051292.1"
                     /translation="KTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKD
                     INFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDY
                     QFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASL
                     SKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKN
                     GGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1749 a          979 c          702 g         1817 t
ORIGIN      
        1 aatatgcagt ttaggctctg aaaaataaga attcactttt attagtccaa tcaaacacaa
       61 ataccgtttt aagtagcttt tcctctactt aaaacggcaa tatgaaagaa actataactt
      121 tttattcaat cttttatgtt taactggttt atcgccaatt ttttaaaact ttgattattc
      181 cttatattat aagaatagac attaagtgaa ctaattcaat catactgatt ctttcgtgta
      241 ttcaaaatta gatgctgttt aataaaatta ttctttttga aaaagaatta taactatatc
      301 ccaaaaattg taggttaatt taaagcctat ttcatcatcg tgattgagct tctttttcat
      361 agacgaattt ctcagatagt ttagttttct ttatgagatt tttgtatatt tctgaccttc
      421 gatacaactc attatgagta ccttgatcaa ctagttttcc ttgatccatc actacaattt
      481 tatttacctt ttcaatagac ttcaagcgat gcgatataat tagtactgtc ttatcttgta
      541 ttaaacgatt taaactttcc tgaatacgtt tttcattttc cgcatctaaa ctggcagtaa
      601 tttcatctaa aattaaaatt ggcgcatttt tcaaaaaagc ccgtgctatg gataggcgtt
      661 gtcgctctcc tccagaaagt gaacttccgt tttctccgac caatgtatga taaccgtcag
      721 gtagtttttc aataaagtct tcgcagttag ctaaacgagc tgcttttttc acttcctcat
      781 ctgacgcttg actatttcca atacgtatat tttccaaaat acttgtatta aacaaagtta
      841 cctcttgaaa aacaatagcg attttgctaa acaaagaagc tggagataca cgtttaatat
      901 catacccatc aattaaaata cagccttcat cataatcata cagtctagac actaatttca
      961 agatactagt tttcccggaa ccactagcac ctactaacgc agtcacttct ccttgtttag
     1021 ccgtaaaaga gatatgatct aaaattgggg tattgttatc atatgagaaa gagacatctc
     1081 gcaactcaat gtcaaaagat ttcagaggac tatctgatcc ctcttggata ctggtttcct
     1141 tcatagctct aattcgctgt attttaggag ctaaataaaa tatttcaagc actgcttctt
     1201 tcatagaatc gaaggaatcc tttattttta ttgcggctag taagtaacca acgacgtaga
     1261 gtatagtcac ttcacccgtt aacagtaagt gaacacctac tagaatgact actgctaaac
     1321 taacatatga gaaaatagag gataaggcca tgactgaaaa agtacttaat tctaccttta
     1381 aatgaatcct ttcactctct tccatttttt tgaaaagtct gtcttgaact tttttagata
     1441 gattaaagct attaatctct tgctgcaatt ctatagtttc ttgaaattct tccgagtttt
     1501 ctctcaaagt atcgtaatat ttagtattgg ctttcgtttg ggatttcttt gataacaaga
     1561 ttaagacaaa actaaataac gttggcaaaa taacagctaa tcccattttg acattaccaa
     1621 ccaaaagcat cactgaaata aaagggaaaa acagagccat accgccggct ttaggtattg
     1681 catgactcat cgcatgctca ataccttcaa cgtcagacat gatagtttga gataaatctg
     1741 ataaattatg ttttgaaaaa taggataatg gtaaatttga caaatcgtct gcaatttgca
     1801 ctcttaaatg cgcactttct ttataagtac tattatataa gttttcatac tccctatcta
     1861 acaaccaata catgaaaagc aaagtaacaa acgatacggc aaaataaagc caacggggct
     1921 ttgtattttc taaaactaat tcgtctatca gcatcattat aatgaatgct ggtaccatat
     1981 taatgcagta tacaaaaaaa ctagaaatag aagctttagt taaagcttct gcgccctgat
     2041 cacttaaagc aaatttgttt ttataaaaac ttttcattgt caagcctcca atcatttgca
     2101 ttctcataat tagtgaccaa ttgtttatac acttgatgtt tagccattaa ttcattatga
     2161 tttccacgtt ctactatttt cccttctgac aagacaagaa tttcatctac agatttaatg
     2221 ctggataatc gatgagctat cataataact gttttttctt ttatcagatg ttgaaaagcc
     2281 ttttgtagtt catactcatt atctggatca atagatgcac tggcttcatc cataataaca
     2341 atctttgcat cttttaaaat tgctctggca atagctatac gttgtttttc tcctcctgac
     2401 agataaactc ctttactacc aattaaggta tgctctcttt cgggaaattt ttctaaaatc
     2461 gattcgcaac cggctaatct tagcgcgtca aatactttac ttgctggcgc ttgaggattt
     2521 gctatagaaa cgttatcgta aatactctga ttgaatagtt tcgtatcttg aaagacaaaa
     2581 gcaattgtgt taataattgt gtcttttgaa tagtgattta aaggcttacc accaatttta
     2641 atctgtccct tatccacatt gtagaaacct gaaattagtt tagctatcgt actctttccg
     2701 cttcctgatt ttcctactaa tgcataaatt cttttttgtt ctaaaactaa gttgaaattt
     2761 tctaaaacac actcttcttt gtatgaaaag ctgacatttt caaatgcaat gtcacaattt
     2821 ttaatctgtg tctcgttccc atattttagt tggtcttgct tcatttctaa ataaagggag
     2881 tctagtgttt cgactgcata tcttgcttca aacaagtgca tacttacata cattattctc
     2941 atgaaagaaa caaaaataat tccacttaaa aaaaacaaca tcaacaagtc aacagctaat
     3001 atcttgggag tggtaagaga agttataaaa aaggttatag gaactatcaa aatgcaaact
     3061 atgccaaaaa atagccattg aaaaagaaca aaaggtgttt tcccactcaa agaatactta
     3121 tatgcaaact tagcataatc gttaattgct ttattcaagg ctttaaatga tgttacttta
     3181 gcaccaaata tctttataac ttgaattccg cggatatatt ctaccgtttc agagcttaac
     3241 acatctaacg attcttgata tttttgaata aaatttgtat tctctgtcat tttctttaac
     3301 aagaatacac caattactaa catggcgatt agaaccaatc caattttaaa gttaatgaaa
     3361 aagcctaagg caactgataa aacaggtgtt actatggctt gagcactatc tggaatcaag
     3421 tgagcaattg ccatatgtgt tttagccgcg ttgtcatcaa ttgtctttcg aatatatccc
     3481 gaagaattta aatcaaaaaa acgaaaactt gcttctgtta aaccgctaat tccctttttt
     3541 ctcaaattag tctctaagcg aaacccaagg atatgagaaa ataagccaga aaaaatataa
     3601 aataaagctc ctaaagttaa ccaagcaaca gtttgaaccg cataactctt agctaaatat
     3661 tcatctccta caataattag agactgaaga aatttgtaaa tactgtaata tccatagact
     3721 gttaatacga cagaaaaatc agaaaataaa gtggctaacc atccgtagta tttcatttta
     3781 gggacataac taaataattt tttgtatact ttcatttttt cctcccccgc attcaatttc
     3841 ttctcttatt cgtttacaac tgttaatgct ttccgtaata gcgtaaatta gagatgtaca
     3901 aacagataaa aggtaaacta cgttctattt tttgtatttt tttaagactt ttcttccaat
     3961 caatacacca atcatagata aacttgctac aactaacact gaaatgattc cccatatacc
     4021 taccgcatag tggatcattg catccattgt tgcagcagat tgtcctttat ctatccaatc
     4081 ttgacgaaaa cgatctgtaa agaaccatat aggaatccac tgtccggcag cactgcctaa
     4141 ttgcattaga gcccaagacg caacttgagg aattgctcga tcatatccaa atacagatag
     4201 aataatatca gctaagatcg ccatactaat cgaactgata aaccaaggta agtacgcccc
     4261 gcccattaac gtaaaaaata acaataaaac aattgtgtaa atagtaaaaa ttccttttct
     4321 agggtagtta tagctaatat aaacaaatat gattccagat aataatccaa aaatcccggg
     4381 ggatatgctg tgaccaaacg ccccaccaat actactaacc attattgaaa aaaagtaaat
     4441 tactagggaa ataactgtag ctaaaccaat aaaaatataa tcttttgttt tcatatatat
     4501 tctcctcaat tctatttcgt tatgtcatta aaaatctgtt ctatcaaagt tgaatcttct
     4561 actctaagtt ttatatcttt ttttattcca ccatttttta aataaataat tctattgcat
     4621 gttttataaa gaaattccgg atcatgagta gctataacta ctgcaccctt ttcttttaag
     4681 tctttaataa ggtcagcaat cctttccata ttaatataat ccaacccact tgtaggttca
     4741 tcaaatagaa ataaattagt ttttgacaat gaagctatag aaaccaataa tctttgtttt
     4801 tctccaccgg ataaatcaaa cggtaattga tctttcattt tataaagttc catttttttt
     4861 aaaatttttt cttgctgatc tttactaact atttcattcc ccagggctaa ctcgctcctc
     4921 actgattctg tgaaaaattg gtaatccatt tcttgcatta ctaaaaacgg cgatgacaaa
     4981 ttttttttat tttcgattat ctttcctttg gtagggcgaa taatcttcat gagtattctt
     5041 agtaaggttg ttttccccac accgtttttc cctaccaaac caataacatc tccaactttg
     5101 acatcaaaat ttatgtcttt tagaatattt tcataagtaa tttcttcaac ttttaataca
     5161 tctgcactta ccttttcgat atagggaact tcaatcgaac tattaaaaat gtcaaatttc
     5221 cttaatgcgt tattgttttc agttttt
//
LOCUS       DAZUMO010000128         5045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000128 DAZUMO010000000
VERSION     DAZUMO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.132"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1033
                     /locus_tag="ACG8ER_002778"
     CDS             <1..1033
                     /locus_tag="ACG8ER_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5051293.1"
                     /translation="SVIVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTDNTLAI
                     LKKYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFESLYNCA
                     IAENADIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNRLYSHN
                     AWDKIYRRSLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYRQQNNS
                     IMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQKNVGGT
                     CEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKMYKTAY
                     NMLWRIYNR"
     gene            1047..2126
                     /locus_tag="ACG8ER_002779"
     CDS             1047..2126
                     /locus_tag="ACG8ER_002779"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5051294.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIY
                     GEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEAL
                     AIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQK
                     GIKLRKELTSKNISNIWYEYLNQFF"
     gene            2145..3569
                     /gene="wzy"
                     /locus_tag="ACG8ER_002780"
     CDS             2145..3569
                     /gene="wzy"
                     /locus_tag="ACG8ER_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFS5051295.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            3615..>5045
                     /locus_tag="ACG8ER_002781"
     CDS             3615..>5045
                     /locus_tag="ACG8ER_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS5051296.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWARKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIIL"
BASE COUNT         1782 a          602 c          916 g         1745 t
ORIGIN      
        1 tagtgtaatt gtaccttgtt ataatgttga aaaatatatt gatgagtgta taaatagttt
       61 gataaatcaa acattgaaag atattgagat tattttgtta aacgatggtt caacagataa
      121 tacgttggcg atattaaaga aatatgaaaa gcttgattct agaataaaag tttacgaaca
      181 taaaaatatg gggttagggc caactagaaa tcgagggatt aaaatagcta atggagaatt
      241 tttggctttt gtggattctg atgattttgt ttccaatact atgtttgaaa gtttatataa
      301 ttgtgcaatt gcagagaatg cggacattgc aattggagaa gtaggcattt tttctgatag
      361 agaaatagaa agagtaaaaa taagaaaaag tctaacgaac ttaaaaacaa ttgaattagc
      421 aacatataat aagaaagaat tttttaagca attctatttc aatcgtttat attcacataa
      481 tgcttgggat aagatatata gacgtagttt agtattagag tttaatttat catttggtga
      541 taacaaaata atttttgcag aagataattg gtttcaatta caagtattta tgacaaatac
      601 aagaatctgt ttcgtagata aattgtgtta ttattatcgc caacaaaata attctataat
      661 gcataagcca aaaagtaatt tggtttttag acatggaaat atgattgctg attatgaaaa
      721 gttgataagg caaaatggaa acaacataat agataaaaaa gcttgtatgt tggttgcatt
      781 tgatgtacta attatggaga tttttaatca aaaaaacgtt ggtggaacat gtgaagatta
      841 taagaatgca ttagtttctt tgaaacatca taaaatattg agagaaaata tttcaaatat
      901 agttagaatg aaagcatatg aacttgaacc taatatgaga aagaagttag cccttgtgac
      961 cattagtata atgtttaatt ttaaaatgta taaaacagcg tataatatgc tctggagaat
     1021 atataatcga taaagaggta ggaaatatga ggaaattgac ctttgtgata ggaattatgg
     1081 gaaaaggagg ggcagaacga gtaattgcga atttggcaaa taattttatt caagataact
     1141 gggaaattga tattataact atatatggga atcggcaaga ctatgaactg aatagaaaaa
     1201 tacaggtaca tccaataata tgtaaaagtt cgtttcgtgc atttagacct ttggaacgct
     1261 ttaaggaact taggaaaaaa ataagggaaa tacagcctga tctggttatt tcgttccttg
     1321 ctgatgtaaa tatccatgtc attatttcat tgttaggagt aaaacaaaaa ttaattgttt
     1381 ctgaacgaaa tgatccacaa cggaatccag aagaaccatg gattaggaaa attagagatt
     1441 ttctttataa aaatgtagat ggaattgttt ttcaaacacc agacgcaaaa aaatatttta
     1501 gtaagatcaa tgataaaaaa gtaaaaacaa ttataattcc caacccaata aaggaaaatt
     1561 taccttatta taataaaact acaagtagta aaaaatttat tactgcatgt agattggata
     1621 aacaaaaaaa tttgccctta atgattgatg caataggaaa acttattcat gaaggatatg
     1681 agtgtgaact ggatatttat ggagaagggg gattaaaaac cgagcttgaa agttatatca
     1741 accataatgg attaacagaa tttgttcatc taaaagaatt tacaaataaa atacatgaaa
     1801 agatgcaaaa tgcggcagga tttgtaattt cttctgatta tgaaggtata tctaattcta
     1861 tgttagaagc actggctatt ggagtgccag ttatcgctac agattgtcca gtaggtggcg
     1921 caagaatgta tttaggtgat agaaatgaaa taggtcatct tgttgaatgt aataatctag
     1981 ataagttaac agatgcaatg cgtagggtac ttttggatag agaaagtgtg gtaagaaaag
     2041 cgcaaaaagg aattaaacta agaaaagaac tgacatctaa aaatatttca aatatatggt
     2101 acgaatattt aaaccagttc ttttaaccaa aaggaggata ttgtgtgaaa gaaaaaataa
     2161 atatattgat gttttcgtta acgttagtcg cgtttacaat attttgcgct ttttcattaa
     2221 atcagaacta ttggctaaat tggcttggaa tattgattta cttatatgca atctgtacat
     2281 ggcattggag aaaaggagat tcaatatttt cattatacac gatttttttc actctttttt
     2341 tattatttag ttatgggcaa tgcattatgt gggcattcgg tattggaaca gatcgtggaa
     2401 tcggtacaac agtagtagct tatggtacgg ggattattcc tagtgaatca gatatgataa
     2461 tggtaaaatg gtattcttgt atttctatgt ttgtatttca cataggagca ctcttattta
     2521 cgagaaaaac cacactaaga aaagttagtt ggtatgaatc tggagatgct gatgccgata
     2581 gaaagttttt atttaaaata gggtgtatca tttctataat agtagtaccg atagtttttg
     2641 tttctaaatt actagaggta cgtatagcat tagtccatgg ttataatgca ttatattatg
     2701 gtgattatgc tacacaaagt ggatatttac aaattattct atatcttttt tttccagcct
     2761 taatatgttt attaattggt agcaactttt caaaagtaat aacgagattt gtttttatta
     2821 tttttgcttt atattcttta ttaggaatta tgtcaggaga cagaggaagt tggttgtata
     2881 gctctattat actcatatgg gcatacactc aatataaagg aacctttaat tataaaaaat
     2941 taataaagtg gcttatttta gctgttatag gaatatatat tctaaacgta ataacgaaag
     3001 cacgtgatgg tggaggacta agtaaactta ctttaaaaga ttttacatct gtatttgatt
     3061 cagatgattc tcctttagtt gattcttttt ttgaaatggg ggggactatg agtattataa
     3121 cttttttcct aataagtggt aatggaatat atccctttgc taatacttat ttgacctcac
     3181 tcttaggctc tatatcaaca agaatgctat caatgtttgg aataaagttt gttttattag
     3241 ctgattggtt ttctcaggat tatttaggaa tcacttgggg gactggattc tcaatgattg
     3301 cagaagccta tgtcaatggt ggatacattg ggggtatagt ctatattttt attattggta
     3361 tagtcatcgg taaaatcctt tctaatagta tagacaacca agatattgaa aatcatccta
     3421 tgaagatgtt tttggctata actacagcaa atattataat gggttttcct agagctgctt
     3481 catatttaat tgtgaaaaat tttgtgtatg gtgttttaat tatattgttg ataaactttt
     3541 tcctacagca atttaataaa cgtctttaat atttaagctt aataaaaaga taaaacaagt
     3601 caggagttgc aaaaatggaa agaactgctc ggctcaaaaa aaacactatt tgggctctgg
     3661 tgtttcaaat aacttctatt gtctgtggta tgatcttgct acgtttgata ttaacaaagt
     3721 acggatccga ggttaatgga atgattagct ctattaacca atttctacaa attatttctt
     3781 ttttacaatt aggagtaggc gctgtaatgc aatcaactct atacaaacca ctagcagaaa
     3841 aggataattt aatgataagt caaatcttag cttctggagg gaagttttat cggcaaattg
     3901 catatattct actagtatac gtgggtttgt tacttgcagt ctattatgtt ctcgttccta
     3961 atgaatttgg gagatcatat acaatggcaa tgattgcaac attaagtatt aattcatttg
     4021 ctcaatatta ttttggtatt gttaatactt tgttattaag tgctgaccag aaaatttata
     4081 ttacgtatat tgcacagaca atagcattta tttctaatac agttgcatgt gctttattaa
     4141 ttagtctgaa cacagagatc cttatggtaa aacttattac tgctataatt tatcttgctt
     4201 gtccaatata ttatagatgg tatgtggatc gaaactataa tataaagagg aatgtgtcat
     4261 atgataagga accgatccct caaaaatgga atggggttgc acaacacgta gcttctgttg
     4321 tactagatag tactgatgtt gtagtgttat ctatattttc taacctgata tatgtatcta
     4381 tatataatgt gtacaattta gtaatatacg gagtaaaaca gctattcatg gctatgacaa
     4441 atggcgttca ggccttacta ggggaacttt gggccagaaa agaaatagaa gttctttaca
     4501 aaacatttgc atggttcgaa tggattgtac atactttgac agtttttatt tttggctgta
     4561 ccggtatact aatcgttcca tttattcaag tatatacaag aggagtaaca gatgctaatt
     4621 ataatcaatt atcattcgca gctttacttg taactgctaa cgcacttcac tgtctgcgta
     4681 caccttatca tgtaatgata aaagcagctg gtaaatataa agaaactcaa aatagtttta
     4741 taattgcttc tttgttaaat ttaggtttat cattaatttt ggttaaacca ctagggctag
     4801 ttggagtagc attaggatct ctaatttcaa tcacatatca tacactttgg agtgcaaatt
     4861 ataattcaaa atatttgctt aaatggccga tgaaaaaatt ctataaacag ttatttgtaa
     4921 acatcttaac tgtagttctt tcttttgggg cgacatcaca attgaaaatg atgtcaatca
     4981 catatttgga ttggattgtt ctcgcaatta aatgtgcagt tattgtattt atatgtataa
     5041 tccta
//
LOCUS       DAZUMO010000129         4898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000129 DAZUMO010000000
VERSION     DAZUMO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.133"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..1734)
                     /locus_tag="ACG8ER_002782"
     CDS             complement(124..1734)
                     /locus_tag="ACG8ER_002782"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS5051297.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1956..2201)
                     /locus_tag="ACG8ER_002783"
     CDS             complement(1956..2201)
                     /locus_tag="ACG8ER_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051298.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2927..4267)
                     /gene="glnA"
                     /locus_tag="ACG8ER_002784"
     CDS             complement(2927..4267)
                     /gene="glnA"
                     /locus_tag="ACG8ER_002784"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS5051299.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4295..4687)
                     /locus_tag="ACG8ER_002785"
     CDS             complement(4295..4687)
                     /locus_tag="ACG8ER_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5051300.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1443 a         1053 c          799 g         1603 t
ORIGIN      
        1 aaaagtcttt tgctttctat cttttttcct acaaataata acaaatcaga aaaagagaaa
       61 agtgacaaga aaattgctta tccccttctc ttgctacttt tctctttcta tcaagttcaa
      121 aaattatttt gtttcgtgat ctgctaatga tgctccgaca aatgctttga ttaatttttg
      181 cgggcgattt ggacgagaaa tcaattctgg gtggaattgg catcccacaa agaatttttt
      241 ctctggcagc tctacgattt ctactaaacg gttatctggt gagacacctg aaaagactag
      301 cccttgttct tcaaacaatt gacgatattt gttattgaac tcatagcggt gacggtgacg
      361 ttcttgcaca acatcggctc catcatatgc ttcagccgtc ttcgttcctt ttttgatttt
      421 acatgggtat aatccaaggc gtaaagttcc acccaaattc tcgatgtttt cctgatcagc
      481 cattaaatca ataatattgt ttgctgtatc tggattcgtt tcagctgatg acgcatcttc
      541 taatccaacg acgttacgtg caaattcgac acaagccatc tgcataccaa gacaaatacc
      601 taaaaacgga acgtcatttt cgcgagcata acgaatcgct tcgattttcc cttctactcc
      661 gcgatcacca aatccaccag gaaccaagat tccatctgcg tctttcaaac gttcttctac
      721 attttctctc gttaattctt gtgctttgat ccaatcgatc tcgatgtctg aattataggc
      781 gaaaccagaa tgtttcaatg cttcaactac tgagatatat gcatctggta actcgacata
      841 tttaccgaca agcgcaatcc gtactttttt cttcaagttc aagacacgtt cttctaattc
      901 tttccattct gtcatgtctg cttctggtgt atctagtttt aaatggtcgc aaacaatttg
      961 atccattcct tgtgcttgta aaactaatgg gatggagtaa agtgtatcta catctggcga
     1021 ctcgataact gcttctggtt cgacatcaca aaattgtgcc aatttgtttt tcacgctttg
     1081 agaaacaggt tgctctgtac gtaccaccaa aatattcggt tggataccta aaccacgtag
     1141 ctcttttaca ctgtgctgcg ttggtttagt tttcatttct ccagcagctt tcaagtaagg
     1201 aatcaatgtt gtatggatat acataacatt atctgtaccg acatctgctt tcatttgacg
     1261 aagtgcttca aggaatggca aagattctat atcccctact gttcccccta cttctgtaat
     1321 gatcacatct gaatcagtca ttttagctgc tcgcatgatt ttttctttga tctcatttgt
     1381 aatatgagga ataacttgaa cagttgctcc caagtattcg cccttacgtt ctttacgcaa
     1441 aacttcagaa tagatttttc ctgtcgtcac gttggagtat ttgttcaaat tgatgtcaat
     1501 aaaacgttcg tagtggccca gatccaaatc tgtttcagca ccatcatctg ttacgaatac
     1561 ttctccgtgc tgatatgggc tcatcgtccc cggatccacg ttgatgtatg gatcaaattt
     1621 ttggatagtt acttttaagc cgcggttttt caataagcgt cctaaagaag ctgcaacaat
     1681 cccttttccg attgatgaaa cgacaccgcc ggtcacaaaa atatattttg tcataagttc
     1741 aaaaactcct ttttaattta tgtgcaggaa aagtctcaga caataaacaa agctccctat
     1801 tcttcaataa gaatagggag ccgaaatttc tgtaaacttc tgaccctcta aaagggtgcc
     1861 caaggtgtat aatacaagct gactaaggga aagtcaagta ctcaaatgaa tactatccgc
     1921 ttacaatttt ttctttttcg atatcctttg agaaattatt tcttaagtcc tttatgagtg
     1981 caaaaaaaat ttaacccaca taataagctt gatataaaca tagctaacgt taaacctaag
     2041 caaatcagaa aatcgttact gaaaaatttt aagtgaaata atagataaaa aaataaaaca
     2101 aacattatga tagataatat cccacctaac aaagaactac tttttcctac ttgcagcaac
     2161 agtttatata gcttatcgta gaaacaatct ttttctttca cccttattta cctcctttac
     2221 gtaaaaaacg tagtccacag ttctcggatt tttggcatat cttttctatt aaataatatc
     2281 aaaaacaaaa caattccact aattttttca aagatgacct ttttttcaac aaaagtgcct
     2341 tgtagggatt cattgaaggt aacgaatcac ataagtaaaa aataaggaga tgcgacaaaa
     2401 gtcgtgcctc cttatttcta aataaacggt gttctcctgc caactcctcg gtcacaagtc
     2461 acaatatcca tcaaacatga gaagctgttt ttgaaattat ttcttgtgat tcggagttaa
     2521 atggtaggct cacaacctct ggataaacgg tattcaaaag ccagtttctc ggtattaagc
     2581 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttaat actcgaaact
     2641 aaacgtcttt ttcaaaacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata
     2701 agttcagcca attcaaaaac aagaaaagct gtttttgctt ggctattctt attacttgtt
     2761 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta
     2821 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc gttagtttct ctaatactca
     2881 ggtcaaaacg ctgatttcac agcctctaat tcacggtatt cctgttttaa taaagttcta
     2941 gatattgttc tctttcccac tcggaaacag tttgtctgaa tgccgcccat tcgagacgtt
     3001 ttgcttcaac aaaatttgag aagatatgtt gtcctaatgc gtcaatcatt acattatcct
     3061 tacgcaattc tttgatagca ttatggatag ttgaaggtaa gtcctggata tgtgcagctt
     3121 ctctttcttc ctcatccatt acataaatgt tacggtcaac agctgctggc ggtgtgatct
     3181 gattacggat accgtctagt cctgcttgca acaatactgc cattgccaag tatgggtttg
     3241 cagatggatc aactgaccgc aattccaaac gtgtggataa accgcgagat tctggtacac
     3301 gtactaatgg cgaacgatta cggccactcc atgcgacata aacaggcgct tcataacctg
     3361 gaacgagacg tttataagaa ttgactgttg gattgcaaac ggctgtgtag gcacgagcat
     3421 gtttcagcaa accacctaag aaatgataag ctgtttcact tagttccaat tcaccgtctt
     3481 tatcatagaa aacatttcct tcatcattga acaaagacat attgcaatgc ataccagaac
     3541 cattgatccc aaataacggt ttcggcatga aagtcgcgtg aagtccatgc ttgcgtgcaa
     3601 tcgttttgac aacgagcttg aaggtttgga tattatcaca agcatctacc acatctgcat
     3661 atttaaagtc aatttcatgc tgtcctggcg ctacttcatg atgtgaagct tccacttcaa
     3721 agcctagact ttctaattcc aacacgatgt cacggcggca attttcacct aaatctgttg
     3781 gtgcaaaatc aaaataacca cctttatcat tcaaggtcgt tgtgatccca ccgtcttcgt
     3841 ctaatttaaa taagaagaat tctggttcag gtcctaaatt gaaagaggta aaaccaaggt
     3901 cgcgcatatc tttcaatgct cgtttcaaat tcccgcgagg atcgccggca aatggtgtcc
     3961 catccggatt atagatatca caaattaaac gagcaacttt tccatgagca ctttcccacg
     4021 ggaagatcat ccaagtggag agatctggat acaaatacat atcgctttct tcaattcgaa
     4081 caaatccttc aatcgaagaa ccgtcaaaca tcattttatt ttctaatact ttgtcgatct
     4141 ggctcactgg aacttctaca tttttgatcg tacctaagat gtctgtgaac atcaatcgta
     4201 aaaatcgtac gttttcttcc tcaatgattc gtttgatttc ttcacctgtt atctgtttct
     4261 ttaccatggt atccctcttc cttttttaat ttattcagtc aaagaattgt ctttgcaatc
     4321 cttgcgactg ataatgacct gttttgctta acccaccttg cgccaaaatc tcatcacgaa
     4381 aaatttgtcg aacgtcatga tctgtcaacg gcttcttgtt ttttgcttct tgttttttcg
     4441 cctgtgccat ttcgtataca cgtttgatcc cagccatatt caatccctct gaaagatagt
     4501 ctttaatctc aagcaacata tcaatatcat tcagcgaata catacgtcgg tttccttcgc
     4561 ttcgttccgg atgaactaaa tcttgttctt cataatagcg aatttgccga gcagacaaat
     4621 cagtcagctt cataactgta ccaattggaa atacggacat agatcggcgc aattcctttt
     4681 ctcccatcag acgtcccctt tcgttttata agttggctaa agtgtaccaa ttaaaaaaaa
     4741 tattgtcaac tgcatatgtt aggttttcta acatgagatc tctatttttt ataaaataac
     4801 aaaaaaataa gaacacaaaa aaggtatcta gttgattttc gtaccagata ccttttattt
     4861 tttattatac tgtactatcc tttattagtg tttttatt
//
LOCUS       DAZUMO010000130         4832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000130 DAZUMO010000000
VERSION     DAZUMO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.134"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1131)
                     /locus_tag="ACG8ER_002786"
     CDS             complement(<1..1131)
                     /locus_tag="ACG8ER_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5051301.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDD"
     gene            complement(1261..2637)
                     /locus_tag="ACG8ER_002787"
     CDS             complement(1261..2637)
                     /locus_tag="ACG8ER_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5051302.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2637..3293)
                     /locus_tag="ACG8ER_002788"
     CDS             complement(2637..3293)
                     /locus_tag="ACG8ER_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5051303.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3303..4580)
                     /locus_tag="ACG8ER_002789"
     CDS             complement(3303..4580)
                     /locus_tag="ACG8ER_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5051304.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4599..4760)
                     /locus_tag="ACG8ER_002790"
     CDS             complement(4599..4760)
                     /locus_tag="ACG8ER_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS5051305.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT         1520 a          876 c          588 g         1848 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgcgcattc tcattagaga acactccctt
       61 ttttgtgact tttctgaagc tagagtgaat actttcaact gcattagtag tgtacatgat
      121 ttttctaatg gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt ttcgtttgca
      181 tacatctact gcaccagaat aatgagacca ccgattttga aaactgtcga aggcagcgtg
      241 tgcagcgttt aaagaagaag cactgtagaa ttttttcatg tctcgacaga cttccttata
      301 atccttactt gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac agcgctgcac
      361 aatgacagaa ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac cgtccatcga
      421 aatgaaaaaa acatcttcta caccacgtac tttcagttca tcaaatactt gcatccagcg
      481 atttttagat tctgtttgat tgagccatag gcctaaaatc tccttgtttc ctttaagatc
      541 atagccaaga atggtgtaca cagcatattc tttagcttca tagttttcgc gtaaagtgac
      601 gtacatacaa tcaacgaata gaaatgcata acacttggct aatggccgag cttgccattc
      661 ttccaattca ggaaggacgg catccgtgat atcagaaatc atttcgtggg aaatatcaaa
      721 gccgtaaata tcttcaacgg ttgcggcgat atcgcattga ctcattcctc gtgcatacat
      781 ggaaagaacc ttaccttcga tgtcggagac atctcgtttt ctattaggaa tcaactcagg
      841 ttcaaaagaa gcctcccggt ctctgggaac atcaatgttt actttaccaa agctcgtttt
      901 aagcgtctta ttgccataac cgtttcgtcg attgtcatgt ttctttggct ctttagaatg
      961 ggcgtcatac cctaaatggt tattcaattc tccttgaagc attttttcaa ataagggacc
     1021 aaacacatct tttaaaacat cttgcatatc ctctacagat tcgggttgat aagtattcaa
     1081 aatggattca gctaacttct cagcatcagg atttcttttc tttctagcca tcgtgtaatc
     1141 acctcttgat cttattttag aaaaataaaa accgtgaagc aaccacccgc ctaggattga
     1201 tggttactta cacggtttat attacactct catttttggt gctaaaaagc accgaaaatt
     1261 ttaattattg tcaattaata attcttttgg attccttctt aatatattga atgaagaaat
     1321 aatcaatgat acaagaagta ctaaactcat aaatataact acataaatca tgaatttagg
     1381 taatacattg atatctaaac ttgataatgt cttgttaaat ccttcagctt ctgccccacc
     1441 acctaattgg ctagatgcag attgtttagc tatttgttta gctatatcac cagtaacctt
     1501 attcaatata ttattcccaa gcttgtcagc tgtatattga gctaagaaat aggaaccaac
     1561 gaatgcgggg attgatataa ataccatctc aattaggaat tgaccaaaaa tttctaattt
     1621 tgatatacct aatgatagta atactgctat ttctttcttt ctagcattca tccataagaa
     1681 taataataat gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata atttatttga
     1741 aattgagtaa atacctgata tagattgttg taatgcaggg taatttgatg aacttttaat
     1801 caaattatat tctctccaat ttatatctaa tttcccaaga tcttttatta cactatcaag
     1861 attcttatca ccttttacaa agaatgttgc atcttggtat acagctgtat cttcggtatt
     1921 accataaact ttagcagctg tatgaatatc agtgattaat gtattttcgt atagttcttg
     1981 tgctgcactt actccaccgc tattatgccc atcgaataga cctttaattt ctacttctac
     2041 tgtttcatct gcacctttct cattatcagc atcaaataaa ttagatttta tttttatctt
     2101 atctccaact ttaaggttat ttttttcagc taaatcttta tgcattaaga ttttattttt
     2161 atcttcattt tctaaatgct ttccttctac taatttatat gcttctgata caaattttgt
     2221 ttcttttgtt gagtcgttaa ccccagttaa cataactgtt cttttaaaat tctttgctct
     2281 ttcaggtgat tgattcgcaa gagtctcttg agtttcaata atatcataat caactaaatc
     2341 tgcaacactg tttattcttt ttacatagga gtctatacta tctgtttgag atatcttttt
     2401 aatatcttca ccttttacat ttcccccacc tctaggtgtt cctggattta cttgtctatt
     2461 tatctccata gaaaaactat ttgttatatt agcaaatgtt tcttttgaag ctctgtccgt
     2521 agcatcttta atagataaac taatgagact taaagttgac atagataaaa taactaatat
     2581 gataattaat gattttaaac tttttctagt tacataagca aatgcatttt ttatcattat
     2641 tcaacctcca aattcatttt atttactttt ttcaactttt taccacttag ttctaaaatg
     2701 atatccgcag aatctgctac ttccttacta tgtgttacaa ctattacaca tttatttcta
     2761 tctttagcta atgtctttaa tatattaatt atttctccag cagtaacact gtctagatta
     2821 ccagtaggct catcagctag tattattgga gcatctgata ccaatgctct agcaatagct
     2881 actctttgtt gctgtccacc agataatttc ataacatttc tttttatttg ttttttatct
     2941 aaacctagtt caaacaagat actttcatct gctgatttat ttactaatct aatattttca
     3001 attggtgata aataatctat taaattatag ttttgaaata ctaaagatat attatttttt
     3061 ctatgattac tatatccttt attttgtata tcttcattct taaacaatat ttttcctgtt
     3121 tgaggtttat caagtccagc aagtaaggaa agaagtgtag attttcctgt tcctgacttt
     3181 cctactatcg cataaaactt cccaagttca aatttttgat ttactcctga caaaactttt
     3241 tctttagaat ttgcataact atatgctaca ttctttattt ctaatatatc cattattttc
     3301 tcctaactta tttttgataa gatttcttta ggcttcttga ttaacattaa tgaagaagca
     3361 aatacaactg ataaaacaat aatacttatt aatattaaat aactttgtcc aagtgttgtt
     3421 atatttgaca taaaactact attatttatt aaacttccac cggaaatcat tgaatcctct
     3481 gagttaatga atccatctac aattactttt agtaatacat ttcctaaaaa taaggaagat
     3541 attatacttg gtatcgatat gaatattaac tcgaatataa attgcattat aatttgtatc
     3601 ttagatgttc caatagataa gaatatacct atttcataaa ttctttccct taaccataga
     3661 atcaagatta atgaaagaac aaccatccca cctaacataa tagaataagt cattactttt
     3721 attatatatt ttattccact cactgactct aaagactctt caaacgcatt agaatctttt
     3781 tcaacaaaat actttgactc atcaatttta agctctttca atttgtttaa ggctaagtct
     3841 gtagattctg cactaccaga atacattaaa attttatttg caatttcatt attttctgaa
     3901 ttatttaata tctcttggct tgttgaataa tctacaaaca ccatattttc actaaaatca
     3961 gaagataatc ctgtatatgt ttcctgtttt ttaccagaaa agatccctat aattttaaat
     4021 ttatgacttt ttatttttcc acttttttca atatctagta attcaagatc aacttcatca
     4081 cctaatttta gattgttttg tttagcaaat tcttcatgaa caataattga attcttatca
     4141 ttttcttcta tatttttacc ttctttaatt gtaaatactc cactactaaa taaaatattt
     4201 cttttagtat tatttgtagc ttcgagtgaa acaacattct taaattcgtc agataaatct
     4261 tctctattta ttctttgttc tccgctaact actttagcat cttttagttt tgctaatcca
     4321 tcatattgaa ttattttttc ttcaatttct tttaattttt caatatcttt aaattgatta
     4381 acattaaaat atttaccatc tttttttgtt attgatattg aagaattaga actttcatat
     4441 aaagcctttt ctatttcatt gcttgatttc attattgtta aacaagaata caagcaagaa
     4501 agaactatag ttaatatgat aaaaattatt aaagttctat ttttctttct tgtaatatat
     4561 gctatagcat tttttatcac tttcctaact actcctctct attttcttac tccataacta
     4621 attgcatcca ctggacaagc atttttacat cgtccacatc tgatacactc cagatgattg
     4681 caattctcaa ctgaatcttt attcatttgg caaacttttt tacattttcc acaattaata
     4741 cacttatcct tatcctgaat cggctacact agactagaca gaaaaaataa ggtgtgtaga
     4801 ataataaaaa acacactgga ggatttttca tg
//
LOCUS       DAZUMO010000131         4796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000131 DAZUMO010000000
VERSION     DAZUMO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.135"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..527
                     /locus_tag="ACG8ER_002791"
     CDS             <1..527
                     /locus_tag="ACG8ER_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5051306.1"
                     /translation="KQYRTKGIAYFSKEDFHELLDIPKSYRESVINKKIIKPIRQELT
                     AIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQI
                     YSEKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPEL
                     KKGLLDNLQNLFKE"
     gene            537..1034
                     /locus_tag="ACG8ER_002792"
     CDS             537..1034
                     /locus_tag="ACG8ER_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5051307.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            1410..1691
                     /locus_tag="ACG8ER_002793"
     CDS             1410..1691
                     /locus_tag="ACG8ER_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051308.1"
                     /translation="MDILVRKIDSKIISEIDRRCQELTTKTSKKWSRNTYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNEYVRTNNQLIETLIGGL"
     gene            1693..2109
                     /locus_tag="ACG8ER_002794"
     CDS             1693..2109
                     /locus_tag="ACG8ER_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051309.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISILSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNHQKETNQTFTAGNFDPTNFLKR"
     gene            2767..3192
                     /gene="bacA"
                     /locus_tag="ACG8ER_002795"
     CDS             2767..3192
                     /gene="bacA"
                     /locus_tag="ACG8ER_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS5051310.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            3222..3386
                     /locus_tag="ACG8ER_002796"
     CDS             3222..3386
                     /locus_tag="ACG8ER_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051311.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            4346..>4796
                     /locus_tag="ACG8ER_002797"
     CDS             4346..>4796
                     /locus_tag="ACG8ER_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5051312.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRL"
BASE COUNT         1745 a          816 c          804 g         1431 t
ORIGIN      
        1 taaaacaata cagaaccaaa ggaattgcct atttttcaaa agaagacttt catgaattac
       61 ttgatatccc taaaagttat agagaaagtg ttataaataa aaaaattata aagcctattc
      121 gccaagaact aaccgcaatc ttcaaaggtc tgacaatcaa aaagaaatac ggaaaaggaa
      181 gaggaaaacc tgtcattggc tatcaattca cgtttaaacc tgaaataaaa aaagcagacg
      241 actttaataa acacgcacca acaaaagaag aacaaaataa agctttagaa ctagttaatc
      301 aacaaattta ctctgaaaaa gaccccactt cattacaaga aaaaatcaaa gaacagaaac
      361 gaaaattaga acttttacaa caattagaag agctagaaag aaaacaaaat aatctgaata
      421 aagaaaataa ggagattaac gaacaaatac cagataacaa ttccgatatt cccccagaat
      481 taaaaaaagg cttactagat aatcttcaaa acttatttaa agaataaggg gtttttatga
      541 cagataaaac tatcaaagaa ttagcagata ttctaggtgt ttcaaaagat aaagtgaagt
      601 atcaactgag gaaattacct agtaatttta cctacaaaaa aggaaatatt atctacctga
      661 aaaaagaagc agtaacaaca attacaaagg ttttatcagg taatgagccg ggtaacttac
      721 ctagtgaatt acctgattca ttacctaccg attacctact caaggagatt gaaactaaga
      781 ataatcatat tgaagaaaaa gataggcaaa ttgaaaaact tcaactagct ttagaaacac
      841 aacaaaaatt actcgaccag caacaacaac tcaccctgca agccaaccaa caaatcgaga
      901 aactgcagga acaactacag ttgacttata cggaagaatc ttcggaaaat aaatcaacga
      961 ctctttctga agaaacagaa tcgatagaaa aacaatcaaa gacggagaaa aaatggtggc
     1021 aattctggca ataaaaccag caatttaaat taataaaaat tgctggtttt attcttcttt
     1081 ttgcttttgc gagcagagca acatgggccc atgttgcgat attttggtgg cgccccaatc
     1141 cccgtttttt cgttgaatct tactcggact gcactcgttt tacagctgat ttttctgaat
     1201 tttgaaagag cataaggaaa cgtggtatca ctttcttacg aaaggtgcta gataagctgt
     1261 gcctatcacg tttctcagaa aaaaatttgc tatcactttt ttgccattat ggatagtgta
     1321 tgtatagtat tttggtaaaa aacagctata caaatactat atttgtatga tatacataga
     1381 ttatacgcac atgcgaaagg aaaattgcta tggatatttt agttcggaag attgattcaa
     1441 aaattatttc agaaatagat agacgttgcc aagaacttac aaccaaaacg agcaaaaaat
     1501 ggagccgtaa tacgtaccta aaagttctaa tcgaaaatga ttttgaccag gcacttaggc
     1561 attacaaaaa agatcaattc gatttattac tcgaaaaatt tatcgatata caagcaaaaa
     1621 atacggaaac actgaatgaa tatgttcgta caaacaacca actcattgaa acacttatcg
     1681 gaggactata acatgagaaa aacttctgtt caaaaacacg ttacccttta tcccagtcac
     1741 gtggacgttt tagattccat tattgaaaga aatccagata tcaacaatta ctcggaagcc
     1801 attcgttttt tatgtatgag ttatgaacct gataccaaca caaccactac tagcaaccaa
     1861 gccaaactaa atgctatggg aaaagaaatt tctatcctgt ccgaaatcat tgcagaattt
     1921 gctgatttat atctaaaaga tactggtgtt cttattggta ccaattctaa ggtgtatcaa
     1981 gaagtgaaag aaaaaattga aaatcaactc aatccttccc gacgaaatca acccaaaaat
     2041 catcaaaaag aaaccaatca aacattcact gctggaaatt ttgacccaac caactttcta
     2101 aaaagataaa aacagaacct gaaaaataaa aaaattcctc gatttctttt tagggggggc
     2161 ggtcttccag caagatttgc aagttgggac caacttgcgg aaaaaaagaa aaattcgctt
     2221 gcgcgaatcc ttctttttgc attttggggc gctgccccaa tccccaagta tcggttattt
     2281 aatagcgtgg caactcaaag aacagaacat actctgaaac aaaaaaagac tgctctaacg
     2341 agcagccagt taaaaaataa tgagtgttta ttcttgaaaa agcaacgcgc ttatgaagcg
     2401 ctaccaaaaa attatcatat tttattaaaa ataacaaccc agtaaaaaat gactggtaaa
     2461 aattcacaaa gaaacggaaa gaagcaaccg cagaaaatca agcttaaaaa gagcttttga
     2521 cctagaccaa tgaaaaatca caaagtccgc ccctgtacct gttggtagct gggctacttg
     2581 gcatactgta cattatgtca acaagaattt aatataacat atttttctag tttgttataa
     2641 caatgccact ctaacaaaaa tgtaagcatt atccttatta aaagctcctt gacaagacta
     2701 taccttttat tttatgattt ttacatagaa aacgttttct ctattttttg tttagaggtg
     2761 atttttatga tttgcaagaa caagaaaaaa attattgcta ttattgctag tttctctttg
     2821 atttttagta ctgcatgtac tgtcttatta acaaataatg ctaatgttga agctgcaagc
     2881 tcttcttaca aacataatca tgctggttat agatgttaca aaatgagtta ttatgtaagt
     2941 ccttctaaat gcaaacaatt ggctcgccaa actaataaac ttaataccgt ctcagctatt
     3001 gcaaacttta ttggactatc tggattaaca ccggggatta tatcattagt tttcggatca
     3061 gcagttagtg ctaataatgt atttgttaca gccgctagaa gaggaaaagg cgttcaatta
     3121 aattatattg cccattttag taataccact acctataatt atagttctaa ccaaaattat
     3181 gttataaaat aattaaaact ttaaactagg aggaatgctt tatgaaatta acaaaaattg
     3241 ctgtaatttt agctgttatt gctggcatta ttttatattt tgtattgcct gctaatcaat
     3301 tcttccttta ctttggaata cctgcgattt taatagtaaa tggcgtaaga atctttgaac
     3361 aacgaaaaaa atgtagctct acctaatcgt tacaaaacaa caatttcacc ttcggtgaaa
     3421 taagcgaaac tttacaaaaa gtggctagca aatgctagcc ttttttgtat tgtagagcgt
     3481 gaattttgag ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcacc
     3541 aaatacctcc gtatttggct agcaatcctt ctaaaaaata cacctatcga tcagagtcaa
     3601 aacaaatcaa ttttcgagtg agcgaatccg aatttgcaaa gctgaagctt tgcaaatgag
     3661 tgtgccggct tccgtaaaaa tacatatggc tataatttgc gttttaaggc gttttaaacg
     3721 ttctggtata taaaattata ccaaaacaac tttaaaacgc cttagatcta attttagagg
     3781 tcttatagaa cattttaggg ttgttagggc ttgccctgac cgcctgtaag gcgctttgag
     3841 aacaagataa gggttttata ctaagactgt cttaaaacgg cttaaacaaa cgctcagaac
     3901 ttttcaaagg agttctttat gaactgtttt taagcaaagg agcctttagg cgactgcgca
     3961 gtataatgtg agagcgtagc gatcacacat ttttagtgac cgtagggagc gagtttccca
     4021 gtttagaaaa attcgcgaac ttgtttgagg aaaaaagcag gagtttttta ggcgggaaat
     4081 tttgcctcct cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct
     4141 gtatgcttac tctcccaagg tgtgcagaat cttttggggg tctttttgtc tgtttttagg
     4201 ggtctttttg tctgttttta ggggtctttt tgtctgtttt tgggggtctt tttgtctgtt
     4261 tttaggggtc tttaattagg ggcttgaccc ctattagaaa gctctttata attttgttaa
     4321 gtagttaatc ttgaaggagg attttatggc aaatgaaatt gtgaaatacc accatgaatt
     4381 aaatacgatt cctttacgca aatttacgtc agtagaaatg aatttattct tctctatagt
     4441 atctagaatg cgtgacgtag gcgataaaaa agtacaattt acattcgaac aattaaaaga
     4501 tttaagcaat tataaagcaa ctgcaaatgt tcgattcata gatgatttag aaacaacgta
     4561 tgacaaatta atggatttac gttttggtag aagaagtgct gacggtttgc aacgtgaacg
     4621 atttgtttta tttaatcaat ttaaaatcga tggaaaagct gatattcctt ttgctgaaat
     4681 acaagtccat gagaaagcct tacccttact taataatctt gaagaatggg ttcgatattc
     4741 tctccaacaa ttcaatgaat tagaaagcag ttattccaaa accatgttta gattat
//
LOCUS       DAZUMO010000132         4728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000132 DAZUMO010000000
VERSION     DAZUMO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.136"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1054
                     /locus_tag="ACG8ER_002798"
     CDS             95..1054
                     /locus_tag="ACG8ER_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HFS5051313.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="ACG8ER_002799"
     CDS             complement(1487..2059)
                     /locus_tag="ACG8ER_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5051314.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /gene="fic"
                     /locus_tag="ACG8ER_002800"
     CDS             complement(2075..2680)
                     /gene="fic"
                     /locus_tag="ACG8ER_002800"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS5051315.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2860..3597)
                     /locus_tag="ACG8ER_002801"
                     /pseudo
     CDS             complement(2860..3597)
                     /locus_tag="ACG8ER_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4040..>4728)
                     /locus_tag="ACG8ER_002802"
     CDS             complement(4040..>4728)
                     /locus_tag="ACG8ER_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFS5051316.1"
                     /translation="QYSNLTLIAEIARYLNFSPLEVQRILTESQDGLEQILFWSNTYK
                     QVVYEILIPKIFEYKFKITKSQITEDNEVVLLKFPQGKNYYKFSGKPELVIMDSDKQL
                     ENYKTKSFHADTYVFDSKPERELFLQYLENDTVEEIFFTGMFTSKQGDFYIQYIDPEA
                     NSVRSYFPDFVVKKRDGSREIIEVKGDNKKDDPVVLAKKAATEEQAGQSGYSYRMILG
                     SEIMNNNYEI"
BASE COUNT         1435 a          916 c          804 g         1573 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc ctctacaaaa ttcacaatag atataatcta ttgaaaattt
     2821 aaaagaagac aaaatagagt acagaaaatg ttttctgtac tctataaatg attctgatac
     2881 ttcattgaag tccatggctt taactaatcc atcacgacgt aataatccat ttgaatgttc
     2941 atttagagcg cgttgtgacg gggtgcctgg atccgcaaaa taaatatcaa tatcattttg
     3001 atttcccata gctttccaat ttgaaaattc tttcccacag tcaaacgtaa ttgatttaaa
     3061 cagatgttta ggaaccgttt gaaaccaatg attaattcga ttttcaatgt ccattgcctg
     3121 tctaccggct ggcttcaatg taataatcac ttttgacaaa cgttccacca atgtaataac
     3181 agcacttttg tgttttcgac cgacaatagt gtctccttca agatggccaa actcgttttc
     3241 aaattctgga tgctccttga tgcgctcatg tatactgcgc cgaaaagctt gtttcccacg
     3301 tttttcttgg tgtccatttg gttttcgttt gcctttcata ggcaaatcac gcacattaaa
     3361 tgcgccagtt tgaaacattc gatacagtgt acggactgaa caatcaagtt tcatttccga
     3421 gcggccgaca atcacatccg gtgtccaccc ttgaacaacc ctcttttgaa catattcttt
     3481 ttgttcctct ggtaaagcaa tcgagcgtct accgcagcgt tttttgtttt ctttgtattg
     3541 ttgatagtag ttcagtatcg tagcaccagt tttcaaaaat tgatacacgt tatgaatcgt
     3601 ttgtcacgca cgtcctaagc attgcgcagt tttggcaacg gattgattat ttttgaaata
     3661 agactctatg attaccagtt cgtctgttgt aagatgggtg taggtcattt gtactcactc
     3721 cttaataatt ctttggtcgg aactattttg agtgtagcac aaatgatctt tttagttgtc
     3781 tagcttaatt ttacaatcga cgaaaagaat actatgatga gattagtttg aaatttcatt
     3841 ttgatgagta gatattagga actgattgat agaacgaata agttatattg atattttgta
     3901 tttgcagtat ttgaaaaagt tgaagatgat aaggaaaata aagaaacaac aggaacagtc
     3961 taacttgact attcctgttg ttgaaaagat tatgattaat ttactacttt attttttata
     4021 gacatgctat ttacatactc tatatttcat agttgttatt cataatttca cttccaagta
     4081 tcatcctgta tgaataacca gattggcccg cttgttcttc tgttgctgct tttttagcta
     4141 ggacaacggg atcatctttt ttattatctc ctttaacctc gataatttcc cgacttccat
     4201 ctcttttctt gaccacaaaa tccggaaaat aactacgaac agagttggct tcgggatcga
     4261 tgtattggat gtagaagtct ccttgtttgc tggtaaacat tcctgtaaag aatatctctt
     4321 cgactgtatc attttctaga tattgtaaga aaagttcgcg ctcgggtttt gagtcaaaca
     4381 cataggtatc ggcgtggaag ctctttgttt tataattttc taattgctta tctgaatcca
     4441 ttataaccag ttctggtttg ccactaaatt tgtagtaatt ttttccttga gggaacttca
     4501 agagaacgac ctcgttgtct tctgtaattt gagatttagt tattttgaat ttatactcaa
     4561 atattttagg gattaatatc tcataaacta cttgtttata ggtatttgac cagaagagaa
     4621 tttgctctag tccatcttgc gattcagtta ggattctttg tacctctaga ggactaaagt
     4681 tcaagtatct ggcgatttcg gcgattaaag ttagattgga atattggt
//
LOCUS       DAZUMO010000133         4581 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000133 DAZUMO010000000
VERSION     DAZUMO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.137"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..310
                     /locus_tag="ACG8ER_002803"
     CDS             14..310
                     /locus_tag="ACG8ER_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051317.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            312..734
                     /locus_tag="ACG8ER_002804"
     CDS             312..734
                     /locus_tag="ACG8ER_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051318.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            753..2345
                     /gene="mobP2"
                     /locus_tag="ACG8ER_002805"
     CDS             753..2345
                     /gene="mobP2"
                     /locus_tag="ACG8ER_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS5051319.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2366..2686
                     /locus_tag="ACG8ER_002806"
                     /pseudo
     CDS             2366..2686
                     /locus_tag="ACG8ER_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2847..3988)
                     /locus_tag="ACG8ER_002807"
     CDS             complement(join(2847..3760,3760..3988))
                     /locus_tag="ACG8ER_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5051320.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            4236..4430
                     /locus_tag="ACG8ER_002808"
     CDS             4236..4430
                     /locus_tag="ACG8ER_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051321.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT         1594 a          747 c          923 g         1317 t
ORIGIN      
        1 ttggaggata tttatgaatg ttaaaattag agatttagat ccaaaattca tttctgaaat
       61 tgatcgaaga tgcgttgaac taagtaatcg tacaggaaat aaatggagtc gtaatgatta
      121 tttaaagcta ctcgttgaaa atgattttga tcgtcctttg atggaataca aacaagaaaa
      181 gtttgaccag ttattagaaa agttttcaga aattcaaagt tataacacaa agatattaga
      241 agaatatgtt agccaaaata atcgtattat agaaatcttg attgagcaaa atagaggaag
      301 tgaactttga tatggcaaag gttatagaca aacacattta ctttcaccaa acacatatcg
      361 atgtattgga tgaaactatt tctactcatg cagaggttaa aaactatagt gaagctgttc
      421 gctttttatg tatgaactat gatccagaag tggagaaaaa agtaaattcc aatcaagcaa
      481 agttgaatgg aatgagtaaa gaaatttcta ttttaaccga aatggtttca gaatttgcgg
      541 atcttcattt gaaagataca ggaatattaa ttggtaaaaa ttcaactgtt tatcaagaag
      601 caaaagaacg agtggaagga aaaattaaat ccaatcaaac aaaaaaatat tcccctcagt
      661 tgaagaaacc tgtgaatgaa tctgttcatg ccagagtaga tccatttgat ccaacgaaga
      721 ttctaaaaag ataaaaatga aggaggataa taatgactca atcgccttca gttattctca
      781 cttgtcaatt tactttgccg aataaaaagt cattttctac ttatattgac tatattacaa
      841 gggaacaggc tcttgaagaa aaggaacgac gtacgcccga agaagaacag gaacttcaac
      901 gaatcaagaa tgctttagag gagttttata ttcctgaagg agaaactttt tcggagagta
      961 agacgggtaa agaattcagt gaaacgtcga atgaagctca atcgttgatg gaacatggaa
     1021 tcaattttga caagttagag gaacaagatt ttaccaatta tctttcttat atgacacgtc
     1081 actatgcgtt agaacaaaag aaagaactga cgcaaagtga acaaaaagaa taccaacgct
     1141 tgaaccaagc aatgaaaaaa tatcaaacag aaccccataa aaaagcttca ggaaatcttc
     1201 ctggagtttt ttctatagct tcagacgaag tgaaaggaag tgatctgaag gagattaagc
     1261 aaatcttcca aagaggacaa caaaaaggct caatcattta tcaagatgtt gtttctcatg
     1321 ataattcgta tcttgaaaaa ataggattgt acgatcccaa aacagatact ttagatgaag
     1381 aagggttaaa agcagctgga agaaaaatga tggagacatt attcgagaaa gaacaattga
     1441 atgaaacagg ttactggatg gcaacgattc accgtaacac taaacatatc catattcatt
     1501 ttgcagttgt tgaaaaagaa aatacgcgta aaccttacac ggaggttgaa aatggtgtga
     1561 gttatatcgt accgaaaggt aagagaagac aagcaacatt agacgcaatg aagaccaatt
     1621 atgtacatga gttagaacgt ttttcttatg aaaaggatcg aatgattggt atggagaaac
     1681 gaaatttatt gacgcggaaa agtgatttgc gtaacacctt aacaaaagtg gtaaaagagc
     1741 cagcgcgtta cgatcgacac gcgatgaact tattaaatga agtatataaa accctacctg
     1801 acaaacgttc ggaatggaat tacggggatg aaaaacgaac caaactttca ccatacacaa
     1861 gagagaaact cgatgagtta accaaatatg ttttaacaaa agaacctgaa tatcaagaat
     1921 atatacagat ttcaaaagca ttgaaagaag aagcaaaaag tctttatggg gaaagcaaga
     1981 gagaatcaaa ggatgcagag aaaaatgctc tctttgattt aaaaaagcgc acaggaaatg
     2041 ccatcctaac agagctgaag agacaggata gtcaaataaa accattaata gaaagtgttg
     2101 atcagatgca atctatttac aatgaacgat cctttcgaac gcttaaacaa ttgccaacga
     2161 atgaatacaa atatagttac gaagaattca tgaagcgcag gaaacaattc caacaaagag
     2221 ttgtcacgca gcggagtttg cgtcaattaa atcgaacgat taatgcccat gaagaaaaat
     2281 accacgcaag taaagcatac gaagagcaac aacgaagaat tcaaatggaa caagaaagac
     2341 agtaaagaca gtaggaggaa tagcaatgaa acaatattta gatcaatgga aagttataga
     2401 aggatcatta agagaagaac gaatcgaaca acttcctgat tgtttagaaa aagagcattt
     2461 gttccagata agagaaatgt tgagaaacga acagtttgat ccaaatcaat ttttagtcgt
     2521 tgagtaccca gctactggtg tgtattgttg taatcatgtg aatggtgaaa aatattttat
     2581 cattcaagaa tatgagggca agttagcacc ttattatact acttgggaaa tgaatgaaga
     2641 gggaatcaat aactttccat gtaaaagcat tggatttgag tcaatacctc ggacacgatt
     2701 tgtgagaatt ttttcgagat tccagcgggt gagtatctca tcgtttcgac cagcggtcaa
     2761 accaaaaccg tttgaccgct gatcgaagcg atgaagttga aaccctgtgg aaatctcgcc
     2821 aaaaaaatgt aagtggtagc taggaactaa gcttgcaatg ccaactcctc catctgattt
     2881 ggtgttaaat agttgatact actatggatt cgcttgcgat tgtaaaatcc ttcaatgtat
     2941 tgaaataacg ctaaataagc agcttcaaag ttttcatacg cttttgatgg atagacttct
     3001 tcttttttga gtgaggcgtg aaacgattcg ataccggcat tatcgtaggg acagccctta
     3061 cgactgaagg agtgccgaat gttcaattca ttcagctttg cctcataggc ttcgctagtg
     3121 tactgagaac ccaaatctga gtggatgatc aagttctcgg agggtgtttg gtttaagagc
     3181 gcattgtcaa gggtttcgat aaccaattct ttcgtcatct gcttgccaaa cttccaaccg
     3241 atgactttct ttgtgtggag gtcttgaata gtggagaggt aggtccagcc atcttttagg
     3301 gtatgaatat aggtaatatc tgtcacccat ttttgattga tcgacgtagt ggtaaaatct
     3361 tggtttaaca ggtttttacg tccttcactt ttaactttcg cttgtttgcc aggcttgtac
     3421 ttcttgaggg tgactgaacg caagcctaat tttctcatca gtatttgtac tttctttaag
     3481 gacaaggtct cgccacggcc aacaagaatc ttatgaattt taggcgcacc atagataccc
     3541 ttgttttcaa agtagattcc tgaaatcaag cggtttaagc gtttgttttc tgattggcgc
     3601 ttggaaggtt tctttttctt ccagtcatag tagctggatt tcggtgcttc caaaacggta
     3661 cacatttgtt ttacgtcatg atcatgaaca tttgattgaa taaattcgaa gacatcactc
     3721 atttttactt ttgagcgaat atggttaaag ccttttttag gatagtgttt tcctcctgca
     3781 tgcgggccat ttcctttttc atcgctagga tttctgcctc agtcatgccg gttgctacat
     3841 tctttgtatg aagcttcttc caacgataaa tcgtttgtgt agcgatacca tattgcgctg
     3901 ccagttcttc gaccgattgg ccagactggt ttaaatctaa gatcatcttc ttaaagtctt
     3961 catcaaattt tttcttggct tttgccatcg tggacacgtc cttttcttag tagttagatt
     4021 ctaccaaatc gtgtccatga aatcatccta acatcacatt gaagagtcca tttcactcac
     4081 agagtgttag ttgttaagtg aataaaagaa gtaaaagagt cctgaacgtg attgtccagg
     4141 actctttttt tcaatgaatt gttttccgcc aactcattga gtggtgcgat ttgcgactac
     4201 attatatcaa aagaaagaga agggaggaag cagaaatggt tcattcaacg attggagttg
     4261 ttgttgaaaa aagcagagac aatctcgttt ttgtgacaga aattcaaaca ggcagatctt
     4321 ttgtcgtcac agacaaagcg gcaaaagctt atcaaagtag cgatatttta acgttaaatc
     4381 tgactacgaa gacgttcgtc gatgcgacag aagattatcc atttgtttag tctaatggtg
     4441 ataagtcaag atgaaattta agattttctt tattgagtaa tatgttttga agcgcacgaa
     4501 actagacccg atcaaaatca aatttttttc gggttgattc aaggatttgc gtcaataacg
     4561 gattgttatt tctctatcac a
//
LOCUS       DAZUMO010000134         4422 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000134 DAZUMO010000000
VERSION     DAZUMO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.138"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG8ER_002809"
     CDS             690..1658
                     /locus_tag="ACG8ER_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5051322.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ACG8ER_002810"
     CDS             1669..2484
                     /locus_tag="ACG8ER_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS5051323.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG8ER_002811"
     CDS             2552..3262
                     /locus_tag="ACG8ER_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5051324.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4422
                     /locus_tag="ACG8ER_002812"
     CDS             3298..>4422
                     /locus_tag="ACG8ER_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5051325.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKML
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1486 a          662 c          922 g         1352 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgcacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaaa aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatgttatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt tttgatttca aggagtagat tttatttatg
     3301 aagcaagtat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatgct tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aa
//
LOCUS       DAZUMO010000135         4318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000135 DAZUMO010000000
VERSION     DAZUMO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.139"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..924
                     /locus_tag="ACG8ER_002813"
     CDS             <1..924
                     /locus_tag="ACG8ER_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS5051326.1"
                     /translation="FKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFF
                     TGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDI
                     RNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDAL
                     IQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVK
                     EIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSD
                     SSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            941..1375
                     /locus_tag="ACG8ER_002814"
     CDS             941..1375
                     /locus_tag="ACG8ER_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051327.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1992..>4318
                     /gene="esp"
                     /locus_tag="ACG8ER_002815"
     CDS             1992..>4318
                     /gene="esp"
                     /locus_tag="ACG8ER_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS5051328.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1588 a          598 c          809 g         1323 t
ORIGIN      
        1 tttaaactaa aaatgaacaa tttgaatgtt gcaatggctg atagagaacg actctataat
       61 tcattgcaaa atttgacttt gtattcttta ggagacattc gagatattct gattttagtt
      121 cattattttt ttactggaaa aatagaagat ctattccatg aaccgttaat tgaatataca
      181 gaaaatctaa gtaaaactat cgagcaaatc aaaatagata atctgttatc acaaaattac
      241 gatccagaaa tatatctgtt tctttatgaa aataaaatac tggaatatgt taaaaatgga
      301 gatattcgaa atttagaaaa catggttttt aatctgagta atggaataat tccaagtgtt
      361 agtggagata cgattcggtc tgaaaaaaat tattctatca ttgtttttga aaagttagca
      421 caaacgtcta taacattagg aatggatata atagaagcgt atcaaagtag agatgctttg
      481 atacaagaaa acgaattagc tgtatcgtta cctgaggtct taaaagtgag ggattctggg
      541 attgtttatt atactaagga aatcggaaaa actaagatag agcatctttc tcccttaatt
      601 tcttctgtcg ttcaattcat cggcttaaat atctataaaa gaattactgt aaaggaaata
      661 gctaattatt tttctgtaag tgaaactaaa ttaagagaaa gctttaagaa tgaaatgcat
      721 attacgattt ataattatat ctctaagcga aaaatttcta cggctaaaat aatgttgaaa
      781 tcaaaccata ctatcagtga agtttcttta ggtttaggat tttcagattc ttcacacttt
      841 tcgagagtat tcaaaaagta cgcaggtgta tctcctaagc aatatcaatt aggtttagtg
      901 gacaatttca acaacattag ctgagaggtt ttattttatt atgagactat atcatttacg
      961 tagtttggag gagttgagga aattggattt taacaaaaaa tttttttggg aaatttctga
     1021 tttgagttgt ttcgtagaat ctgagcgtct aagtaatggt aataatagtg tgttaaatga
     1081 atatgacaaa gtagtaatta ataaagtaaa aagtttatgt gaagaagcta gagaatctat
     1141 cagtcatatt aatggtagca attttaaaca aacattattt aatctgatta agctagatgc
     1201 taaaatatca agttatttat tctttttgat acatgatgaa tttatggatt atcaaaaatt
     1261 ggatcagtta attgaaagag aaagctggga agattattac gtgggtctaa ctttttcaga
     1321 aaagttaaac aactacaagt taatagatta taagtatttg agtgaaagca attaattatt
     1381 catgtagagt actagacact ttaaatatgg acaattcaat tataaagaaa aagaatcaat
     1441 aatttaatga ggctgtgaca aatgtcattt tttgtttcgc taaaaacgag tgaaaaaatg
     1501 ttgataactc caatcattcg tttaagattc taggaatcat tcagaaatgc tgtgaaaccg
     1561 atactttata attataaaga aggtgatttc aaattccgaa gaaggaattt gaagtcacct
     1621 ttttacttat gtcacagtct cattcagtaa tgcgggataa ggctccttat tccgttattt
     1681 gaatactctg aagtttaatc taagaaaggg taatattttg aaaacatgct tcatcgcata
     1741 gaaatgaact taatttcaga aagaatacat acatatcttt ttatatgttt tcagctgctg
     1801 aatggttaaa aagcacaaat atccgaaaaa aacatgaaga aatgtacttt ttttgttgta
     1861 atgttcatga aattctctaa gtaaattagt taacataagg ttagtaagat tcctattctg
     1921 tgaataatac gtatcagatt tatcttttat tcttactagg aaagtaatta ttaagggagt
     1981 tgatttgaat aatggttagc aagaataata agagagtatt tctagaaaaa acaaagaaac
     2041 gagtattgaa gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt ttgattggtg
     2101 ttggtcttgt cttgggaaca actgaactag ttaaagcaca agatgaaatt tctccaagca
     2161 gcccacttga aacagcgata agctccgtac aagtaggtga caaagtagct tcggggaata
     2221 cttttcagga gaatcctggt tatacgaaaa attataactt ttcggattta caatttagtc
     2281 ctcaagaact aactggagac acactaaaag gcaatacaat tggatttgaa gtttacggaa
     2341 aacataatat tgcagcatca actaaaaact gggaaatccg tcttcaatta gatgaacgat
     2401 tagctaaata tgtagaaaaa attcaggtcg atccaaagaa aggaattgga tcttctagac
     2461 ggacctttgt aagaattaat gattcgctgg gtagacctac aaatatttgg aaggtcaatt
     2521 atattcgtgc aagtgatgga ttatttgctg gggcagaaac aaccgatacg caaaccgctc
     2581 ctaatggggt aattacgttt gaaaagagcc tagatgaaat ttttaaagag ataggcatag
     2641 acaatctcaa gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat caagacgatg
     2701 ataaaattgt accgggtatt gatagtacgg gctacttttt aacagattca gatgatttct
     2761 ataatagctt agatgtatct gaaaataatc ctgaccagtt taaacatgga tcagtaagtg
     2821 caaaatatga gaaacctaat actcaaacaa aagatggttc aggatccaca ggtgctaatg
     2881 gagctataat cttagatcat aagttaacga aaaattataa tttctcatat tcatcttccg
     2941 caaagggcac gccttggtat gctaattaca aaattgatga acgattagtt ccatatgtag
     3001 cgggtataca gatgcacatg gttcaggctg acaaagtaac atatgatgtt tcgtttgaat
     3061 ccggaacaaa agtagctgat ttagcaatcg agcgacggga aggtcatgaa aattatggca
     3121 taggttcaat aactaataat gatttaacta aacttatcga ttttgctaat gcaagtccac
     3181 gtccagtcgt tattcgatat gttttacaat tgactaagcc attagatgaa atcttagaag
     3241 atatgaaagc aacagcacaa gttgaagaaa ataaaccatt tggtgaagat ttcatctttg
     3301 attcttggtt gtcggatacg aataaaaaat taatccagaa cacttatgga acaggttatt
     3361 attatttgca agatattgat ggtgatggaa accctgacga taaagaagag agcggagaca
     3421 cgaatccata tatcgggaaa cctgaattag aagaagtata tgatgttgac acaacagtta
     3481 aggggaaagt attcatccac gagttagcgg gaacaggtca caaagcccaa cttgttgata
     3541 aagaaggtac tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat ggggctccaa
     3601 tttcagatac tgtagaattt gaatttacgg gtgtagattc aagtaaatta atcgcgaaag
     3661 atgaattaaa aatccaaatc gtttctccag gttttgataa accagaagaa ggttcaaccg
     3721 ttattaagga atcaccaaaa gcggttgata aacaaaccgt ggtagttgga tttaaaccag
     3781 atgctaaaga atcaattcgg aataataaaa acttacctga agatgcagag tattcatgga
     3841 aaacagagcc tgatacttct aacgttactg atagtacgaa aggtattgta actgttaaga
     3901 tcggaaatcg aactttcgac gtggatgtag agtttgctgt aaaagcttct caagctatgg
     3961 aaaatgatgc aacatacgta cctataacaa caactccaga aacgacagtt caaagtggaa
     4021 aacctacatt tgataaacca gatgttcctt tagctaaaga tgccttttca atttcagatg
     4081 tctataataa gtacttcggt aatgcaagtg ttgatgcaaa cactggtatt gttacattca
     4141 ctccagctaa aggtgtagga gaatcggagc cgattactgg aacaattcct attaaaattg
     4201 tttaccaaga tggttctgta ggcacgaccg atttagcagt aactgtaagt aaagatattt
     4261 atgaaaatcc aggagaaaac attcctgcag gctaccacaa agtaaccttc accgcagg
//
LOCUS       DAZUMO010000136         4293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000136 DAZUMO010000000
VERSION     DAZUMO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.140"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..790
                     /locus_tag="ACG8ER_002816"
     CDS             <1..790
                     /locus_tag="ACG8ER_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5051329.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="ACG8ER_002817"
     CDS             887..3136
                     /locus_tag="ACG8ER_002817"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5051330.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..>4293
                     /locus_tag="ACG8ER_002818"
     CDS             3139..>4293
                     /locus_tag="ACG8ER_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5051331.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1392 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact aga
//
LOCUS       DAZUMO010000137         3878 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000137 DAZUMO010000000
VERSION     DAZUMO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.142"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACG8ER_002819"
     CDS             complement(718..3867)
                     /locus_tag="ACG8ER_002819"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS5051332.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYAQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          804 c          636 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gcgtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAZUMO010000138         3826 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000138 DAZUMO010000000
VERSION     DAZUMO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.143"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1086
                     /locus_tag="ACG8ER_002820"
     CDS             <1..1086
                     /locus_tag="ACG8ER_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5051333.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1304..2446)
                     /locus_tag="ACG8ER_002821"
     CDS             complement(1304..2446)
                     /locus_tag="ACG8ER_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5051334.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2451..2636)
                     /locus_tag="ACG8ER_002822"
     CDS             complement(2451..2636)
                     /locus_tag="ACG8ER_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5051335.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2990..3235)
                     /locus_tag="ACG8ER_002823"
     CDS             complement(2990..3235)
                     /locus_tag="ACG8ER_002823"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051336.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3229..3630)
                     /locus_tag="ACG8ER_002824"
     CDS             complement(3229..3630)
                     /locus_tag="ACG8ER_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5051337.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1256 a          697 c          593 g         1280 t
ORIGIN      
        1 gatatcaatg atgatggctc aagaaaaaaa gtggagaaag ttggcggaga cacccgacca
       61 gaggccgaag ctgctttacg aaaagtttta tcagatattg acgaaacagg acaatacttt
      121 ttaggtacgg atactcgagt aaaacaatac cttgattttt ggatggagga atacgttaaa
      181 ctaaatctaa aatacaatac ctatgaaaac taccgattta ccatcaaaaa tcatataaac
      241 ggttatttag gaaagaaaaa acttacggat ctctcccctg ctcttttaca aaatttcatc
      301 aatgctgaat ttaaaaaggg ttactcgaag aaaacaatga ctattactca ctctgtcctt
      361 aagaatgcgc tgaatatggc ggtttatcct tggggattaa tcaagcaaaa tcctatgctg
      421 tatgtaaaaa taccaaaata tgaagcacga ccaacgacta aaaaagatct aaaaatcatt
      481 tctcttgagg actttgatca tatgctagaa attactcctg aaggacatcc tttctatatt
      541 cctttgaata ttggatttta tacgggaatg cgcgttggcg aagtttgtgg tctgacgtgg
      601 gatgatgtcg atttttcaaa tggaacaatt actgtagaga aacaaatggt aaagaatgac
      661 ggcgcatggg tatatggtac gccaaagaca agcagttcca atcgaacgat ttttattgga
      721 caaaccttgc tagcaattct gaaaaaacat aagaaacaac aattagaaaa tcgaatgaag
      781 tatggaaagc tctacattga ttcaaatgca gtatgtacga aggaagacgg tgagctagtt
      841 acgccaagtg tagtgaaatg gaatactagg aggatatcga atgcactctc cctctctttt
      901 aacttccatt ctctcagaca tactcatgct acacttcttc tcgaaaatgg cgcaaaaatg
      961 aaagaaatct ctgaacgatt gggccacagc agaatttcaa ttacgatgga tacttactcg
     1021 catgtaacag ataagatgag aaatgaaacg gtcgatatca tggaaaatct tagaaagaat
     1081 tcttgatttt tgccaccgaa aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa
     1141 catggtttat tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc
     1201 gtccattttc gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa
     1261 aaataggcta tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg
     1321 taatttcatt tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag
     1381 tagttttaat agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt
     1441 caattaaaaa ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg
     1501 caatttcagc tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat
     1561 caaaagaaaa aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt
     1621 ttaaatcttc aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg
     1681 tttcagcaaa ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc
     1741 tgccatttat taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac
     1801 ctgtaacaaa aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat
     1861 aaacactatt atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct
     1921 gagtttttat tttacctata gtatctacag gattttcttc aataatttca agtacaattg
     1981 cccttttaaa aactactctc aacttattga atatctgttt aatataattt ggtgataatg
     2041 gttgctcaat gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta
     2101 tatgaattgg tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta
     2161 aaatatgatc tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa
     2221 aatactctcg ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag
     2281 aaaaactttg ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc
     2341 ttctggtcac ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc
     2401 aagtgcctct ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc
     2461 caagaaatat ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta
     2521 cgataaaaaa agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta
     2581 gcatagtact gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata
     2641 gcaccttcct tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg
     2701 aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta
     2761 aatcatcgct ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac
     2821 agcaagaaat catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat
     2881 ctatttaatt ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac
     2941 tattagaaga ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt
     3001 taaattttaa gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta
     3061 cataggtaac gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat
     3121 aagaatgata atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata
     3181 agataatgga tagtggaact aataggtact gccgcttttt agatttatct accataggtc
     3241 ataccccatt ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct
     3301 catattgaga attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat
     3361 aatctttctt cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat
     3421 taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata
     3481 cctgccttca tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt
     3541 tcgaatagac ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg
     3601 tatagatatg tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac
     3661 taatacaacg taagcgcccc atagaacagt accttactaa caaatgcacc tcaagatata
     3721 cttgagctat caagaagtct ggaggttttc tatgtataaa ttaaatgaga ttgttcctgt
     3781 ccaattagga aacgaaaaga gtgcaccaga aaaaggtaaa cccgcg
//
LOCUS       DAZUMO010000139         3789 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000139 DAZUMO010000000
VERSION     DAZUMO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.144"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..533)
                     /locus_tag="ACG8ER_002825"
     CDS             complement(147..533)
                     /locus_tag="ACG8ER_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5051338.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(801..950)
                     /locus_tag="ACG8ER_002826"
     CDS             complement(801..950)
                     /locus_tag="ACG8ER_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051339.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            1412..1624
                     /locus_tag="ACG8ER_002827"
     CDS             1412..1624
                     /locus_tag="ACG8ER_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051340.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(1902..3701)
                     /locus_tag="ACG8ER_002828"
     CDS             complement(1902..3701)
                     /locus_tag="ACG8ER_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS5051341.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         1296 a          675 c          652 g         1166 t
ORIGIN      
        1 cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga ggctgacttc
       61 gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca ctcacttcga
      121 gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt tacagatgag
      181 ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct tgctttcaac
      241 tgcaagatcg ttccttcggt gtaagcagaa ccattggcag agtcaccaga agtaatgaca
      301 tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt gtatttaaaa
      361 cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg cattggagct
      421 ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc ccaacctttc
      481 catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt catgaaagat
      541 ctcctttttt atttttatta tattaaatat tatacagata ataacggaat attatcatcg
      601 aaacagctaa caaaattgtc ataaaaaacg agatggttga tattagaact tgtgtttttg
      661 tttaggtcta gagataaaga gagacaattt ccaaatatat gtaatgaata caaaaagacg
      721 tttgtcgcgg attaccgtta caaacgtctt ttattgattt tccgagatct catcagtcgc
      781 catttggaat ggttcactcc ttatctagat tcgtttaatg ctcggactgc ttgttcgatt
      841 gtaataccaa acgcaacgtt attttcgttt tgtgcatcaa tcgccataaa acggttagaa
      901 gttgtatcaa aagctacgcg gtattgcata tttgtttttg taaacgtcat aaaattcatc
      961 tcctttgtaa tgtaatttag ttcttgaaag tttcctttaa actttcgaac ttataccaag
     1021 tttagcatcg tacgtttttt gatgcaatta aaaagcattt atcattttcc ttatttattt
     1081 tttcttaaat ggtttagcta ttttctttta atccgataag tagcccaatc atcaaggtta
     1141 caaatactat tgcttgaata taaatcatat gctcacctct ttataaataa cttagcaata
     1201 taaagaaaaa aacgcacgaa caacatagga atataaacaa acgtttttgt cacaacgtct
     1261 aagatcgtgt taaaaaatag ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa
     1321 aaaatgaata aaaacgtttt caaatacaag gtaatttgtt attataaaat aaagaaaaaa
     1381 caaaataaaa ataaagaaaa gagggaatga aatggtaaaa gcagaagtag gaagatctta
     1441 tatatgcaaa ccgatcggag tatctcatga actaatcggc tgtattgaac gtacatatat
     1501 caacacggct gtcgtgtatg ttgaaagtta tgaccaacga gactatcaac ttatcaatga
     1561 aagcaagtat cgcatgctcg taaaattctg tgatattagc gcacctactg aattggtttc
     1621 ttaaacagta tttttgagaa ttcgcaagaa ctcccttata atataaataa agacagaagg
     1681 gagcgagaag atatgccaaa aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg
     1741 atctctgcaa ttcctgctat tttagctatt gttcgtgcga tgaaggaatc aagagaaagt
     1801 taagcacaca ttatgagcgt caaacaaaaa acgcatatac ctgcggtggc aggttatgcg
     1861 ttttttgttg tataaagact tttttcatta acgatttttg attagccgac tcccaaaaca
     1921 aaatttagca gtttatctga ataagtgatt tcttcatctg tttgtttcac actttcattt
     1981 agtagtccca tctcatcggt tatgatacca tccacaccat aaaacatcat gcgactcatg
     2041 gcatctgaat cgttgattgt ccatacatat acttcttttc cgtcttggtg ggcagaatta
     2101 atgaagttac ggttgatcgt actatattcc attgtcagaa aatcagcagg cgtcacaggg
     2161 ggcccaacaa tattgaaagg taagatgtag cccacataaa agtttggttc tgcttctttt
     2221 aaatctgaaa cggtttggta cgtcaaactt tgcaaaatat gatggttctc taagataact
     2281 ttgcggtatt tatggacaaa tcgttcgaca aggtcatccg aatctttttt agaggtcttg
     2341 atctcaataa gtaatttttg gtttagctta tgtgcttttt ccaaataaga atcaaaagaa
     2401 caaacagggg cttgcactcc gttttcagta actgtaagat ttgtcaattc tttcaatgta
     2461 agatcctgtg gtgttccatt tacccctgtt aagtgacgaa gatttgcatc atgcatcact
     2521 acgaaatgac catcttttgt ttcctgaaca tccatctcta cataatcagg atgatagtct
     2581 ctgcttgttt tttcaagtgc ttctaaagta ttttgcgccc cattttgggt actcacacct
     2641 ctgtgagaga cagtcactgg ttttgtctga acagctgaag tcagataatc catattgtaa
     2701 agacagacgc caataccgaa aaaaactgca aaaagagtca aaccagtgtt tttcagcgca
     2761 gagaaacgca agttgggggc ttgtgggata aaccacttag ggatctcagg caagaagcct
     2821 tcgtcatcca tgaaatcaac aatgatataa aaaatcccta cggtagacat cacgatattg
     2881 aataataaaa tcccttgtaa aagcgtcata gcaaacacag cgctgatcaa tgcatagtct
     2941 gggaaaaatt gttcaaccat actttgtgcc aaaataacaa cgatatatcc taaagaagaa
     3001 aggagtaaaa tcgtaccgcc aatcacaata aattggcccg tgatggccaa aagacgcgat
     3061 ttggtcaatg accagctttc ccgaatggct gctcgaaaag aacgatctcg tagaatcatt
     3121 tccggtaaag caaaaatcag tcgaatccca atatagccaa ggaaaatata aacgagtaaa
     3181 aaggaactga cgatgatcca acgattagca aaaataaaat ccataatgaa agcggggatt
     3241 tttatttttg acagtagatc agaattaaaa cttaatccgc tgatcggtag aatcaataaa
     3301 aaataagcaa ggaaaaacaa aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc
     3361 aagtgtaaga gttgcttcaa agagattggt tctttctttt taataaaaaa aacactcatc
     3421 aaaagaaaag taaattcgaa ataaactaac agtaaaatcg tcatcaaaac tacgattaac
     3481 gagagtagaa ccccaggatg ttgagaaaaa attgaaggaa tattgtcata agacaaatag
     3541 tcaattgccc ctcggtgtaa aataaaacgg gtcgtactgc tcaataaggg taaacaaata
     3601 aataaaataa agccatgcat cagcaagaca tcacggaaat aagcttgtgt tccttttaaa
     3661 aagtcccaag cattacctaa gctacgtttc aaataattca tcctatcacc tgctcttcac
     3721 attgtagcgc aaatctaatg aaaaaggaaa gggtgcagtg tgttttgcag accctttccc
     3781 agatttaac
//
LOCUS       DAZUMO010000140         3765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000140 DAZUMO010000000
VERSION     DAZUMO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.145"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACG8ER_002829"
     CDS             <1..525
                     /locus_tag="ACG8ER_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051342.1"
                     /translation="LEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..2055
                     /locus_tag="ACG8ER_002830"
     CDS             586..2055
                     /locus_tag="ACG8ER_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS5051343.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2066..>3765
                     /gene="topB"
                     /locus_tag="ACG8ER_002831"
     CDS             2066..>3765
                     /gene="topB"
                     /locus_tag="ACG8ER_002831"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5051344.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1392 a          638 c          832 g          903 t
ORIGIN      
        1 ttggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagctg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt
      241 cctgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt
      361 ttaaaaggaa aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatt cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
      661 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
      721 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      781 ttagctaaac tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca
      841 gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt
      901 gagacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      961 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
     1021 gggttgattg ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac
     1081 cgtttagatc atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg
     1141 gcaagttatc aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa
     1201 cacattgaca agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag
     1261 catttaaaat tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag
     1321 aaactacaag atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat
     1381 tacgttgaag agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa
     1441 atatgtgtgg acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga
     1501 atcgttgatc cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg
     1561 caagtaccta aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa
     1621 cctgaagcaa ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg
     1681 gtgtctgctc atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta
     1741 gaaacaattg atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa
     1801 cgcgcagatg gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac
     1861 gttaatgcag gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag
     1921 catgaacatg aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag
     1981 atccagcaac aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa
     2041 caagaaaggg agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca
     2101 agcgaaggct tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat
     2161 ccaagatcga ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga
     2221 gttagattcg cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc
     2281 tattttcccc aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt
     2341 cgtcaaacga cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga
     2401 aggggagctg attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt
     2461 taaaagactt tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt
     2521 acgagacgca gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga
     2581 ttggttaatt gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat
     2641 accaggaagt ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt
     2701 gcaggaaaag attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat
     2761 cgctcaaaat ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga
     2821 agcatttgaa gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat
     2881 ccttcaagtc gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag
     2941 ccttcagtct aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat
     3001 gcaagggttg tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac
     3061 ggaaggggaa tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc
     3121 cgaagcgatt ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt
     3181 ccaggaacat tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca
     3241 actaagtcca ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt
     3301 tgcggaaaag tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt
     3361 aaaggcaatc gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag
     3421 cgacggaaaa gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt
     3481 gacggtggat ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg
     3541 tacgttgatc actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc
     3601 catattgaaa gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac
     3661 cttgaaacaa aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg
     3721 gattttgctt tgccaagccg tggaaaaaga gccgctgctt acgag
//
LOCUS       DAZUMO010000141         3531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000141 DAZUMO010000000
VERSION     DAZUMO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.146"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            246..441
                     /locus_tag="ACG8ER_002832"
                     /pseudo
     CDS             246..441
                     /locus_tag="ACG8ER_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(1973..2491)
                     /locus_tag="ACG8ER_002833"
     CDS             complement(1973..2491)
                     /locus_tag="ACG8ER_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS5051345.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2858..3106
                     /locus_tag="ACG8ER_002834"
     CDS             2858..3106
                     /locus_tag="ACG8ER_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051346.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1220 a          593 c          518 g         1200 t
ORIGIN      
        1 ttatttcaaa aaagcagata aataggtata gatttaataa tttaaaataa atggtataca
       61 tgaataggaa aggaaacaga aggaggcaat cttaattttt taaattgtct aaattatata
      121 gaaagaacaa tctttctata taattagaca taagagggac tacggttgat ttgatattaa
      181 caagcgtata tgaatgtata tacaaataaa aaaggggagg gagaagttaa agaaaattca
      241 gagaagtggg gcactcagta gtattaacaa tttaaaagga atttcttaaa gaaaatgggt
      301 tgcatcctgg agatacaatt tttttagata aagaaaaaat tcaagcaaca attcaaaaaa
      361 atcttaactg ctgagattga tgtagcaata tctcaatcat caacaaagta cgatcctgcc
      421 tttcaaaagc ttattgataa atgtttattc attataaaaa taaaagaaaa atttatgtca
      481 gcttttttca tcaaagtggt aatgataatt cgtctattaa ttgtattttt taggaaatat
      541 ggtataattg ttttaacgaa aaggtaattc atgatacacc aaaaaccact agctggcggg
      601 ctagtggttt tcttttacgt tctattcaga ttaacgggta ggcttactgt cttttttggc
      661 aataattttg caatcaagcc atttacagat gtaatgcgcg gatacttctg ctaagattgc
      721 aaaaaacata tcgaaaaggt aattcataat acaacctcct ccccttgcca taataggccg
      781 gggtagcaga cagcataaat attataccat aactgaaaat ttcattttag tacatttcaa
      841 tatattgatg aaccagcaca ataaaacatg ccatgactaa cagaaaaaaa cttattaaac
      901 cagatataac ttgtaaacat ccaactatat agaaaacgta aattttctat atagttggat
      961 taagataacg actatttatt ttatcatatt tttactatac cctatacttt tacttggtat
     1021 atttatgaat aatcgcaaat ttacccctat actactagtt tgtaagttag attaataaaa
     1081 atactaaagt tatttgttct tttattaaat acacctacaa cttataaatt gatggtattt
     1141 agataacacg aaagaataaa aataacagct gatagtaaag tttgttgacg attgctactc
     1201 aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa acttatgaaa tgaaagacct
     1261 tttacatctt gcagaaatcg gtactgggat taaactaagc aataaaaact aatgttcccc
     1321 tcattctttg aacttctact atttgattta tttattctga taaatgacga tttcaccttc
     1381 aggatcttca aatcccgtat agaatttatt taagatctct tcagtatcag taataggata
     1441 ggcgataggc cacataccct ttgatggtta gaataaactg ctcaaaatct gtatcgtatg
     1501 ttggctttag catattaaca aaggctatct ccaaattata aaccataagc gttgaatcgt
     1561 tcttaacaat ttcaattctt cctaacccaa agtcagaaga tataattacc ggacttgcct
     1621 tacccttcat gtgggttgtt gagattccat atttaaaggg aattgtcggt actatcggcg
     1681 gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg ctagtctcct aagtcccttt
     1741 gtagcggata tggcaccata atctcatttt atcgaagtga gtcatggttt atctgttggc
     1801 caaccaaaat tgatacaatc taacgatgca ccctctacgg cattctaggg gagtgcactt
     1861 gcattcaccc aataaaaacc ggcagttagc aaagaactta tgtagtcgta tgttcttact
     1921 gttaacatac aagaggtctc ctttccacaa ttgcttagca gtaaacggga atttacaaag
     1981 ctaatctctt ccattttctt atctttgttc taaaagtccc aaatggagca actgtattta
     2041 catgaataaa tttatatact tcccatacag ccgttttggt tgcctcgtct gcccattttc
     2101 ttttgtgtgc agtgaataga tcttcatcac tcattgaatc aatcatcatg ttaatatcat
     2161 caatattacg attgagaatt tcctctaatt ctttcaatgg tagatgtgca taggtatttg
     2221 taaaccactt atataactct cctaactgat tccatttaaa ttgatcagaa ggcgttttaa
     2281 cagataatcc atttctttca tcattttccc attttagaat taaattcgtc caaccaactt
     2341 gatatgctaa attttcagca ggtgttctat ccacttcctc aactctttta tccttcaatt
     2401 cttcaggaat agtttcaaat tctaaaatat atttagtata agtcctattt atttcttttt
     2461 ttaattcttc tttattttta tatgttctca ttatattacc tccataaatt cctaattgtc
     2521 tcctttaatt catctataat ttcatccacg atattttaat ccgttctaca atgcatatct
     2581 gatatgcatt gactgtctaa ctcatatgtg aatgatttat aaaatatccc aacgtctcat
     2641 ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac gaatatattt cttttgtagt
     2701 aaaatcggac tggtttgcat atcattttct tcgtaaatat caaaatcatg ttaactacta
     2761 tcaattacta ataaaaacta ccactttaag cgttaagaac tgttaagttt agttaagtga
     2821 ggatagttca caaaatccat tgttaaactt cacgaaagtg gtaaaactca aaatgcttta
     2881 tataggatta cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt
     2941 aaaaattgac gatgacacgt tccttacggc tcatcagatg aaacagctac aaaaacgaaa
     3001 tgatcaactc gaagaaaatt taattctaaa aaagcgattg ccatattcac gcctcgctcg
     3061 acgaactatt aaaggtcaag atttacctga agatcatcct ttttaggaaa ttgaagcttt
     3121 ttatgttgca caatgcattt ataatactac aatgatgttt tcaccagata ttcttctagg
     3181 gtgcggtgta atgaaacaga tacacttgca aaagaaaatt caccgcgaat ttactaaaat
     3241 tatgaataat tatgttaatc ttccgaaatt tttttccaaa attaagggat caagctggtg
     3301 ttatagattg tttaaggttg gcaaaaaaat aacttctttt taaacaatta tagaaaaccg
     3361 atactctgtt aagttggttg acacggtgtc gatttttatg actttcttat tctgaaactt
     3421 cactaacgga cagtacctaa aaagtctata gtatggacgt ttacgagtac tattttatca
     3481 aaaatgctgc tcatttcaac ttatatgttt tagtgaacta cccccactta g
//
LOCUS       DAZUMO010000142         3409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000142 DAZUMO010000000
VERSION     DAZUMO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.148"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG8ER_002835"
     CDS             complement(<1..2232)
                     /locus_tag="ACG8ER_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5051347.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG8ER_002836"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG8ER_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          944 a          756 c          547 g         1162 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtattgcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagttttact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtga
//
LOCUS       DAZUMO010000143         3360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000143 DAZUMO010000000
VERSION     DAZUMO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.149"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..234
                     /locus_tag="ACG8ER_002837"
     CDS             <1..234
                     /locus_tag="ACG8ER_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS5051348.1"
                     /translation="GVRSSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQD
                     RFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            194..439
                     /locus_tag="ACG8ER_002838"
     CDS             194..439
                     /locus_tag="ACG8ER_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051349.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            436..855
                     /locus_tag="ACG8ER_002839"
     CDS             436..855
                     /locus_tag="ACG8ER_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5051350.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            852..1409
                     /locus_tag="ACG8ER_002840"
     CDS             852..1409
                     /locus_tag="ACG8ER_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS5051351.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1406..1702
                     /locus_tag="ACG8ER_002841"
     CDS             1406..1702
                     /locus_tag="ACG8ER_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051352.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1779..2192
                     /locus_tag="ACG8ER_002842"
     CDS             1779..2192
                     /locus_tag="ACG8ER_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5051353.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            2501..2653
                     /locus_tag="ACG8ER_002843"
     CDS             2501..2653
                     /locus_tag="ACG8ER_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051354.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(2650..2925)
                     /locus_tag="ACG8ER_002844"
     CDS             complement(2650..2925)
                     /locus_tag="ACG8ER_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051355.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
BASE COUNT         1179 a          499 c          709 g          973 t
ORIGIN      
        1 ggagtgcgaa gtagcaggca ttggaaagca gcagcaaaca ttgaatttta ttacaacgaa
       61 attcaaaaag ggttcgatca gatgcgtgag ctggatcggc aaacaaattg gagcaagaaa
      121 cttcatcaag atcgtttcaa atttgtagag aagtataaag aaatattaga agagtatttg
      181 aggaggacag caaatgataa aaaaactcgt tcaattcagc atggatttat atgatatcga
      241 atcaggagca acactatctg tggaatcgga ccatctaatc ataaattttg gtggaaagcg
      301 ccagattatt ttgtgggtag ttgatgatgt actgtttcca gaaattgttc atgatttcga
      361 agaatcaaaa gcggttgagt ttgaaatagt gaaaaaagta atggaattga ttgaaaaata
      421 cgaggaggac agcgaatgat accgaagttt agagcgtggg ataaacgaaa gaacgtaatg
      481 agagatgtag ccgtcttgca ttttactaaa aacggcaaaa caaactttat tgaatattgg
      541 ataaatccta ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat gcaatccaca
      601 ggaatgaaag ataagaatgg tgtggatata tttgaagggg atatagtatt agtcagcgtg
      661 cgaaatggct tcgattactt agataataaa gtctgtattg tcaaaaattc aatagattat
      721 tccggattag tttgtgccac tgttgatgaa gacttagagt atcaaatttt taacacagag
      781 ctgtttgaag aatacacgta cgaagtcacc ggaaatatat acgagaatag cgagttattg
      841 gaggaacagc gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt aaagcgatag
      901 gaaatgatgc aatagctgct tgctataacg agagtataaa ttcaggtatt acgttaatga
      961 aaaaaataga cgaaccgcag aaaccagtta ttccacaact cgtggccggt tggcttgaga
     1021 aatctacgga cccttttaca aaagctgaaa aaatagcgta tttaatcaaa tctaaagatg
     1081 gtgattcata ttatttctgt gattggtttg tacgagatgg catagtgacg caagagcaag
     1141 gagaagaatt actcgcttgg gcaacaagac aatcatatga aacactattg agcctataca
     1201 acggctacga ggttgagaaa gagccgttat gggcaataaa gaatgccgat ggaaactatc
     1261 ttactaaatg tgctttatgg ggaaaagatg gagtaaatta tagttttgaa tgcaatccat
     1321 ctcatcgatt gcttttcact gataaagcaa cagcggatgc tgcagcattg ttggtgaatg
     1381 gaacagtgga agaggtggta gaaagatgaa actaaaagac ggattttacg ctagcagtca
     1441 cggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac gcaagaaatc
     1501 aaaattcaaa gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt atccgttcag
     1561 aggatacgtt aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa atacaatgaa
     1621 gtgtgatcgt tcgactgcaa aacacaagca ttatttagct gtagcgagat tagttgacat
     1681 ggaagtaatc aagagcaaat aaaaaaagcc ggatcgctcc gactgattca ataaatccaa
     1741 cacatttatt atatcacata aaggagcggt ttgacttgat gcaattgtta cgagaggtag
     1801 atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg cgtttggagc
     1861 ggatggcagg tcgctctttg atagatatta agtcgccgat tataacggat atgccgaagg
     1921 caccgaagca tggcaataag gcagaagacg cgattattca gatgatggat atagaaacgg
     1981 agagagatgc gattctagcg gctttgatgg ctcttagtct gattagtcgt cagatactct
     2041 actacagctt ctgtgtgcca gatagcttct caaactacag aattagccgt gaagtgggtt
     2101 attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt gcagaagcat
     2161 ataaacacgg aaaaataatt gcttataaat aatttggcgg ttttttggcg gaatgatggc
     2221 ggtttttaga catttaccag tgttattatg gtagtgtcga aagattagga aacaggatcg
     2281 acaaaataaa cgaagggagg caatctccct catcgtttta aacttcttct tagatagaca
     2341 gcaactaatt atgaagaaaa ggatgtgaat tcaactcctc ctaaattctt catcggttgc
     2401 tgtttatttt gattagacag cagcactcac aaaactaaac atgttcttgt cgtctttcaa
     2461 ctgctgctgt ctattaattt atgtattgga gggaagaaaa atgaaattat cagtagaagg
     2521 cactccagaa gaaatacaag aattgctcca agctattggt agtagcaagg agcaaaaggt
     2581 aagtgtttct aattcagata ttgacggtat ttgcaattgt ttaaacagag aaaaaatatc
     2641 taatccttac taagagaaaa tatgttcgtt aatgaatttc caattttcct ctgtagctct
     2701 accttgataa tttttttgca gttcacagat gaataaggaa tcgttattat caagtactgt
     2761 atttaatttt tcacacattt gatttggatt caaattagtt ttaactaacc aaaaagattt
     2821 ttgtagcttt atatatgccc caaattcttt tattatttca aatactttat catacttttg
     2881 ccctgggtta ttaagatcat aactaatcat gtagggttta tacatgttgt cttgcacatc
     2941 cttgttttat atttcagcgg accactcgct gataaataaa attatacgct tagtatttat
     3001 tttcacaata ttaatttatc gtaataactt ttgagtaaat agaacaaaaa acctgcacta
     3061 gtttccgcta gtacaggcag tgactatatc ggttgataat ctagcatata ttaaaaataa
     3121 ttgcaagaaa agaaatttat agtggtttat accaaattat caaagcaatg ttattttgtt
     3181 gctgtctatt gttttttaat tattcacacg ataactaaag gtgggtgaag agaaatgatt
     3241 ccattaataa tttcaatttt tgcgctctgt cttaatgtct atatgattgg atttaaaaat
     3301 gggcaaaata aaaaatagta gcagccaaga ataattttat agtgtcactg tggcggaaag
//
LOCUS       DAZUMO010000144         3108 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000144 DAZUMO010000000
VERSION     DAZUMO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.150"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..243
                     /locus_tag="ACG8ER_002845"
     CDS             <1..243
                     /locus_tag="ACG8ER_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFS5051356.1"
                     /translation="CTSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQK
                     GSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            259..408
                     /locus_tag="ACG8ER_002846"
     CDS             259..408
                     /locus_tag="ACG8ER_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051357.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            482..1075
                     /locus_tag="ACG8ER_002847"
     CDS             482..1075
                     /locus_tag="ACG8ER_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5051358.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1091..1990
                     /locus_tag="ACG8ER_002848"
     CDS             1091..1990
                     /locus_tag="ACG8ER_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5051359.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            1993..2478
                     /gene="ssb"
                     /locus_tag="ACG8ER_002849"
     CDS             1993..2478
                     /gene="ssb"
                     /locus_tag="ACG8ER_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5051360.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2844..3104
                     /locus_tag="ACG8ER_002850"
     CDS             2844..3104
                     /locus_tag="ACG8ER_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051361.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         1149 a          510 c          602 g          847 t
ORIGIN      
        1 tgtacgtctg aagcctgtga tttcaaacta tggacaacga ttgccaaaaa gaaacttaca
       61 gcgacacagc tgaaagagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta
      121 aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc
      181 tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa
      241 taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa
      301 aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa
      361 ttgaagaacc agcagcggat tcaaaaagaa atcgaattag atcgataaat tcctttcttg
      421 ttaatataaa actaacatgt tatagttgtg ttagttttat attaacttta ggggaagagt
      481 aatgaaagac aaaatccaat gtatttttga taaacataat ggcaacctat ccttgaagga
      541 tgctagaagt gaaggattat cgcctatgac gttagatcga ttagccgaaa aggataaaat
      601 agaaaaggta gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg aattgtatct
      661 cgctcagagt atctattcca gagggatttt ttctcatgag actgcgctcg atctttttca
      721 agtaggaaca tatatcccta aaaagatcaa tatgatgttt cctaaaggat acaatgtatc
      781 aaaggagaaa ttagatcagt atgccattca accgcattac actaataaag aaaattttga
      841 cctgggagtc acagaaacag aaagctttta cggcaataaa attttagtct atgatttaga
      901 gcgaacgtta tgtgacatgt ggaatccaag atacaaagca agcgtagagg taaaacaaga
      961 agcattaaaa gaatatatga caagtaccag tagaaataca aataagctaa gaagatacat
     1021 ggatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     1081 gtaaagatga atgacaccga cacaatttaa agagaaaaca agaaatttcg cagcagagaa
     1141 acatttaaac ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaatgtat
     1201 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga ttggatcaaa
     1261 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     1321 aacaaaagaa aagttagaaa ctgttttttc tgaattaaca gctaatccta caaaagatgg
     1381 aattcgtttt gaattaaagg gattaacaga gacgagggaa gccgattatt atccaggttt
     1441 tcaagcaaag tttattgctt atcttgaaaa tgcacgtgta ccttttaaat tagatattac
     1501 ttcaggtgat tctattattc cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga
     1561 taaaacgatt catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc
     1621 cacgtttagt tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat
     1681 tccgaaatta gaacgtatcg atgagaaaaa tttgtataag agtgttcaag gaacattcaa
     1741 ggaaagaaat aatcaatcgg tgtttaagca tttgtatgat caggaaatgc ctcaattggt
     1801 gaaagatgag ggcttgagag aaaagtggaa aagataccaa caagaaaact attttgccaa
     1861 agacatttct ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga
     1921 gcaagagaag gcagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat
     1981 ggagagataa agatgatcaa taatgtaact ttagtagggc gattaactaa agatccagat
     2041 ttacgttata ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt taatcgtaat
     2101 ttcacgaatc aaaacggcaa tcgagaagct gattttatca attgcgtgat ttggcgcaaa
     2161 ccagcagaaa cgatggcgac attagctaaa aaaggcagct tgatcggcgt tgtcggacga
     2221 attcaaacac ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg
     2281 gccgataact tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat
     2341 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac
     2401 aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat
     2461 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     2521 aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     2581 tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     2641 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     2701 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     2761 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     2821 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     2881 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     2941 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     3001 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     3061 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataa
//
LOCUS       DAZUMO010000145         3102 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000145 DAZUMO010000000
VERSION     DAZUMO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.151"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(367..729)
                     /locus_tag="ACG8ER_002851"
     CDS             complement(367..729)
                     /locus_tag="ACG8ER_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051362.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(803..1183)
                     /locus_tag="ACG8ER_002852"
     CDS             complement(803..1183)
                     /locus_tag="ACG8ER_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS5051363.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1265..2440
                     /locus_tag="ACG8ER_002853"
     CDS             1265..2440
                     /locus_tag="ACG8ER_002853"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS5051364.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2516..3100)
                     /locus_tag="ACG8ER_002854"
     CDS             complement(2516..3100)
                     /locus_tag="ACG8ER_002854"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5051365.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          966 a          591 c          511 g         1034 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
       61 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      121 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      181 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      241 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      301 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      361 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      421 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      481 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      541 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      601 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      661 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      721 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      781 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
      841 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
      901 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
      961 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1021 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1081 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1141 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1201 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1261 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1321 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1381 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1441 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1501 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1561 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1621 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1681 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1741 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1801 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     1861 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     1921 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     1981 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2041 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2101 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2161 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2221 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2281 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2341 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2401 acttaatcga atcaagtatg ctttagagac aatcgattaa atatttattc aaaataaaag
     2461 gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt
     2521 ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt
     2581 gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat
     2641 gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga
     2701 atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa
     2761 agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt
     2821 tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca
     2881 ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa
     2941 tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg
     3001 ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg
     3061 gtcctttttc actttttaaa tctagttgtg ggaatgccat at
//
LOCUS       DAZUMO010000146         3089 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000146 DAZUMO010000000
VERSION     DAZUMO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.152"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..814
                     /gene="fabI"
                     /locus_tag="ACG8ER_002855"
     CDS             59..814
                     /gene="fabI"
                     /locus_tag="ACG8ER_002855"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS5051366.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(924..1976)
                     /locus_tag="ACG8ER_002856"
     CDS             complement(924..1976)
                     /locus_tag="ACG8ER_002856"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS5051367.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2102..3022
                     /locus_tag="ACG8ER_002857"
     CDS             2102..3022
                     /locus_tag="ACG8ER_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS5051368.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          898 a          623 c          589 g          979 t
ORIGIN      
        1 attattctct atttttttac gactatcaaa tttttatcag aaacaaagga gagtcattat
       61 gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc
      121 ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa attatctata cttaccaaaa
      181 tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg
      241 tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg
      301 ggagatccac ggccttgtcc atgcagttgc ttacgccaac aaagaagaat tgtccggaaa
      361 tgtgtctgat atttccagag acggctatca attagctcaa gatatcagca gctactcttt
      421 aattgctgta tcaaaatacg cacaaccgtt tcttgcacca aacagcggga tcgttagcat
      481 gacttatcta ggttcagaac gcgcaatccc gaattataac atgatgggta ttgcgaaagc
      541 ttctcttgaa gctgctatcc gttatttggc agctgagttc tcgcctaaag gaattcgggt
      601 caatggaatc tcagctgggg caatcaaaac attagctgtc acaggtgtta aagactatca
      661 aaaactgatc cagttatctg aatctcgtac gcctgatcag caaggtgtga cgatcgaaga
      721 agtcggtaat gcttgtgctt tcttaatcag cccactttcc agcggaatca tcggtgatat
      781 tatctacgtt gacaaaggtg tccatctttc ataaactttt gttgcattct ttaaagtaaa
      841 acatataaaa atcaggaaaa aactttttaa agtctcttcc tgattttttc tttttacgtt
      901 tttttcaact cattactttg aaattattcc acgacttcaa tccagccttc tggtgcttct
      961 atgtctccaa attgaatacc tgtcaattca tcatacaacc gttttgttac tggtcctact
     1021 tccgtttcac tataaaatac atgaagattg ttattgtgat aaatcccacc gattggagaa
     1081 ataacagccg ctgtaccaca tgcaccagct tctgcaaatt gatctagatc atcaatgaaa
     1141 atatctcctt caattgcttg cagtcctagt cgttcttcag ctaactgcaa caaggaatat
     1201 ttcgtgatac ttggtaagat agagggcgat tgcggagtga tgaattttcc gtcttttgta
     1261 attccaaaga aatttgctgc acctacttct tcgattttcg tatgtgtggc tggatcaaga
     1321 taaacacaat ctgagtaatc cagttctttt gcttctgttc ctggaagtaa actcgctgcg
     1381 taatttccgc cgacttttgc tgcaccagtc cccttaggag cagcacgatc gtaatcagat
     1441 acaataaaat tcgttggttt tagtccacct ttgaaatacg ctcctactgg cgtacagaag
     1501 acgacaaata aatattctgg agctggatgg acaccaatgt tttcaccgac cccaatcagt
     1561 aaagggcgca agtataatgt agcgcctgtt ccatagggcg gcacaaaatc ttcatttgct
     1621 tttaccactt gcttgacagc atcaacgaac atcttttcag ggaccattgg catcagcaat
     1681 cgtctagcac tttgattcat ccgtcgacta ttttgttcag gtcgaaagag attgaccttg
     1741 ccgtcttttc gacgataagc ttttagacct tcaaaacatt gctgaccata atgaagcgct
     1801 ggagagcctt catgaatcgt caggtaatta tcttctgtca attctccttc ttcccattgt
     1861 ccgtctttcc atttggcaat aaaacgccaa ggagttttga tataagaaaa acctagattg
     1921 ttccaatcga tttgcatata cttaccacct tcgttatttt ctccacattt tatcattatc
     1981 catattattt gtcattttat tttcagcttt tttccttcat tatagttatt tttgctattt
     2041 ccttttttga aaacttcttg gtacaataca gaaagaatca aattttagaa aagaggattt
     2101 tatgttttca ttattagcaa ctgccggtac gacagactct tctggcgaga cattgacaga
     2161 tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg gacgggattg attgggacgc
     2221 cattttggct gcctttatcc aaaaaagttt atatctgatt tttttgatcg tccttttttg
     2281 gattcttcac cgtgtgggaa aatatctgat tgataagtct tttaatcagt attctaaaaa
     2341 agccattcta aacgagagcc gcttaaagac tttacatagt cttttaaata ctatttttca
     2401 ttataccctt ggtttctttt ttatctatgc aattttatct gctatcggtg tgccgatcgg
     2461 atcacttttg gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg cccaagggtt
     2521 catgagtgat gttattaccg gattttttat tatcatggaa caacagatgg atgttgggga
     2581 ctacatcaag ctagcaaact taacgattga aggaaccgtc gtatctgttg gtatccgtac
     2641 ccttcagctg aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa acatcaccac
     2701 gatcagcaat ctctctcgtg caaatatgca agtactttta gatattcgga ttgttcctga
     2761 agaaggatat gacaaaatct atgagatcat tgatcgagta aatcgtacat tagctgaaaa
     2821 ataccaagaa gagctccaaa ctgaaccttc tatctttgga ttagtggatc ttggaaacag
     2881 caattttgcg attcgtacgg tctgctacgc attgaacgga aaacaatatg ccttaaaaga
     2941 agagttcttg agctcctatg tcaaagaatt gacagcttct ggtttcacga ttccgaacag
     3001 tccaattgct gtcggaaaat aatttggaca aacgatgcaa cgaatctcac taaaaagagc
     3061 gtgggacaaa agtaaagaac acttttgtc
//
LOCUS       DAZUMO010000147         2999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000147 DAZUMO010000000
VERSION     DAZUMO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.154"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1051
                     /locus_tag="ACG8ER_002858"
     CDS             155..1051
                     /locus_tag="ACG8ER_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5051369.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1143..1358
                     /locus_tag="ACG8ER_002859"
     CDS             1143..1358
                     /locus_tag="ACG8ER_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5051370.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1376..1648
                     /locus_tag="ACG8ER_002860"
     CDS             1376..1648
                     /locus_tag="ACG8ER_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5051371.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1650..2513
                     /locus_tag="ACG8ER_002861"
     CDS             1650..2513
                     /locus_tag="ACG8ER_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5051372.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1182 a          479 c          520 g          818 t
ORIGIN      
        1 gataaataga ggtctttcca ctactatcta aatggaaaga cctctatttt tcatatagga
       61 gctacttttg ggtcagccct atatattaat cacaaatcac ttatcacaaa tcacaagtga
      121 ttaatcacaa atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa
      181 agaatggggc gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat
      241 tttaaacaat aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg
      301 aaagtccaaa ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt
      361 aatgatcgat aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt
      421 agaattgcca cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat
      481 tgttcatttg actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc
      541 aaaattaact cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc
      601 acctttaaaa agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta
      661 tacaaataat gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga
      721 aagtacaaac aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa
      781 ccctggacta gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat
      841 aaaatcaaac ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa
      901 tattatcaag cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa
      961 aggctatgac aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg
     1021 aattattcaa ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa
     1081 atcacttgtg atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt
     1141 gagtgattgt gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg
     1201 caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta
     1261 taaaaattga aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag
     1321 aagaatatat acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc
     1381 agttacgtat gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta
     1441 taatcgagta ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact
     1501 agaagtgaat cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc
     1561 atttgaagaa ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca
     1621 aattttgaca aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc
     1681 aatttgaaga tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg
     1741 aatcgccaac cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat
     1801 cggcaatttc cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac
     1861 aacagcaccc taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg
     1921 ttacccctta ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt
     1981 ataatttagt gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa
     2041 tgcttcaagc caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact
     2101 catatttagg aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca
     2161 gggcaacacc aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa
     2221 cccttcataa aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac
     2281 tctattcaag cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa
     2341 atcgtaaagt atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg
     2401 ttcaaaatca acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct
     2461 tgcagccacc tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa
     2521 aaaagtaaaa gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc
     2581 tcatttttta aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt
     2641 tggtacattt tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc
     2701 gaaaacacaa gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt
     2761 gcgttctcgt cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa
     2821 aaagaaagga gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt
     2881 cacttcctca aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa
     2941 gaggaaaaga aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZUMO010000148         2962 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000148 DAZUMO010000000
VERSION     DAZUMO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.155"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..1304
                     /locus_tag="ACG8ER_002862"
                     /pseudo
     CDS             261..1304
                     /locus_tag="ACG8ER_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(1509..2828)
                     /locus_tag="ACG8ER_002863"
     CDS             complement(1509..2828)
                     /locus_tag="ACG8ER_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFS5051373.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          991 a          493 c          563 g          915 t
ORIGIN      
        1 taaaacagca tagttctcaa attgtttaaa atgattgtca agttaaaaaa atctgggatc
       61 atataaaata gtatagttct caaatatgat tagaaaccct attaaagagc aatattaagc
      121 caacttactt aaatcgttta attaagaaca gtaatctctt ttagtcagac ttttagtctg
      181 acttttttgt atttccaaaa tttattcttt acaaaaacga acatatgttt gtataattgc
      241 ttgtgaatgg gggaataaaa atgtttgatt acagtagaga accggtgaat gactattttt
      301 taatcgacat gaagtctttc tatgctagcg tggaatgtat tgaacgaaat ttagacccaa
      361 tgactacacc gttaattgtc atgagtagat cggacaacac tggttcaggt ctgactctag
      421 cttcttcacc tactgcaaag aaaaaatatc atattactaa tgttagtaga gctactgatt
      481 taccttatcc attaccaaaa gaacttcata ttgttccacc acgaatgaac ttatatataa
      541 aaaaaaatat ggaagttaat cagattttta gaaagtatgt cgcagatgaa gatttattga
      601 tttattcgat tgatgaatca attttaaaag tgacaaaatc cttaaattta tttactagtt
      661 caggaacaag aagcgagaga agaagagaat tagctaaaat aatcaaagat gagattcaga
      721 aagaaacagg tctaatagca acagtcggaa taggagataa cccattatta gctaaactcg
      781 cactggacaa tgaagcgaag agtagtaaag aatttatagc tgagtggtct tatgagactg
      841 tacctgaaaa aatatggaaa ataaacgaga tgactgactt ttggggcatt ggatcacgca
      901 tgaaaaaaag attgaatcat atgggaatta tgagtataga agatttagca aattggaatc
      961 cctacacaat taaaagtcag ttaggtgtaa tcggacttca gttatatttc cataccaatg
     1021 gaattgatcg tacggatatt gctattccac cagaaccaac caaagaaaag tcttatggga
     1081 attcacaagt tctaccaaaa gattatacga ggagagaaga agtagaacta gtagtcaagg
     1141 agatggcaga gcaagtagcc attcgaatta gacaacacaa ttgcaaaact ggttgtgtac
     1201 gcttagcaat tggtacgtca attttggaag aaaaatcagg tttctcgcac cagatgaaaa
     1261 taccaattac agataatacg aaagaattac aaaattattg tctttcctga ataattcata
     1321 gctttaattc tttggcacat tttattgaaa tatgtatcac taatatccct atcaactgat
     1381 tttgactaaa aagttacttc aaacattcac tgttcaaaaa tattgaggaa taaattttgg
     1441 gtgtaaggca ttttgagcat acttctccct ggataacttc ccagtaaaaa aatcgttaga
     1501 ttgttggatt agatccattg actggctcgc ctcaggcgag caaagaccct gtagaattca
     1561 ttctgataca catgataact agataatttg agatagactc gtctacccgt ttggactaat
     1621 ttcccagcaa ctttcagaaa cttcaatcga atagaatgaa tggtcattcc cttttggact
     1681 tcttcaaagc caatcgttct taagaagttg actaagttgt aagctatcaa gctgagcatc
     1741 attcggacat gattctccaa aaagcgagga ctgtctgttt tgtcaaagta aaagccagct
     1801 ttcgcttctt taatgaagtt ctccattgtg ccacgtttgg catagataga aaagatggta
     1861 tcaggagaaa cattttctga tagattcgtc acgataaatt catgtcgaaa gagtagttcg
     1921 cccgcttcac gtgttgagcg tatacacacg cgacgacttt gtgaccagga ttgtgcttga
     1981 taagtggtcg agaagtactg aacttctcgt tcttcccact tttgattatc gccataaagt
     2041 actgatttct cagctatttg acctagtcta cgattattct tcagtcgaat aacataatga
     2101 ctcttttttg attcacacga atcatacaca ccaggtgtag cgaacccgct gtctccacga
     2161 accaagatgt cagtgtttgg tagagagtga ttatagtgct ctaataaagg tgtgagaaac
     2221 tcctttacgc cttttgatgt atattgattt cctgaacgta gttcagcttt taagaagtca
     2281 ccagtcaatc cgtcaaaagc taccaatgga tggtaaccat aggtttggta gtgtgtatta
     2341 taattcgttt gttcttggtg accaaatgta tctgaatggg tcgaatctaa atcaatgatt
     2401 aattccgtat cattacggat gaggcgtgct ttatcgataa gttcttggtt caaagcttga
     2461 agttcatgga tattctcctc agatagtcga tctaaaaatc gggaaagtga agattgagaa
     2521 gcgagttctt tcctatctaa aacagcttta aacacaggat cttgtctcag gagattagct
     2581 gcagaatcag ctgagtaacc agcaattaat tgcataatga actgctcaag tatggataag
     2641 ttgtcatgag taaaatacgc tcgttcgtct tcaatgtgaa tgtgttgctt agccagttca
     2701 gaaaaaccaa agatgttcat cagctctttc actaggacga gacccgaatc actcgataat
     2761 tgaccacctg tatgagaaat agtgatattt gaattgaatt ttacttggtt tttgtgtaag
     2821 ctagtcatga aaagaactcc tttcttttgg ttggtttagt cactttaacc atagcagaaa
     2881 ggggttcttt tttcatcact taacgggtga agatgaaaaa gtaattgtaa gctgccgtga
     2941 ggcagtttca catggtttta at
//
LOCUS       DAZUMO010000149         2936 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000149 DAZUMO010000000
VERSION     DAZUMO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.156"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1226
                     /locus_tag="ACG8ER_002864"
     CDS             <1..1226
                     /locus_tag="ACG8ER_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS5051374.1"
                     /translation="KQQFKAATDFILKNEDKTERVENLHFETEKYKDHPVDYQPLTEK
                     GRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNFLVRDDRLDHAVAFLWKDPQTK
                     ETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSY
                     AALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIF
                     YEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKT
                     ENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDF
                     DRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPEVKKDWNDQLKHEIQQQEIRKQ
                     KRAMLFQQGRQQERE"
     gene            1237..>2936
                     /gene="topB"
                     /locus_tag="ACG8ER_002865"
     CDS             1237..>2936
                     /gene="topB"
                     /locus_tag="ACG8ER_002865"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5051375.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1070 a          528 c          642 g          696 t
ORIGIN      
        1 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
       61 gagtggaaaa tcttcatttt gaaacagaaa agtacaaaga tcaccccgtt gattatcaac
      121 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga
      181 ttgattatgc agaaaaagaa gggttgattg ccgaactaaa gccaaaatat gaacggcaaa
      241 atttcttagt tcgagacgat cgtttagatc atgcggtcgc ctttttatgg aaagatccac
      301 agacaaaaga gacagtaggg gcgagttatc aaggaacgtt cattgattat gaacgttttg
      361 gtgagagagg cacatataag catatagata aaaattcgac agcgaatcat ggctttaacc
      421 taaagatcgg tgaccctaag cagttaaaat tttttgagag tagtattgat ttacttagct
      481 atgctgcact gaatcgtgat cagttaaacg atacgtggtt agtctctatg gaagggttaa
      541 aacaccatgt gatcagtcat tattttggtg aggcagtatc tgaattgaga aagaaacaag
      601 cctttcctca atccatcgag atttgtgtgg ataatgaccg tgcaggacat attttctacg
      661 aaaaagaaca attaatgggc gcagtcgatc catttacgaa tcagaaagta cgttgtgaac
      721 gaggaatcgc taatgattgg caagtgccaa aagaatacaa aatcatttat gaagaagtcg
      781 caaaagaaga aaaggtcaca ccagaagcta ttatggccat acacaagaca gaaaacaatc
      841 tgcaattaac gaaccaactg gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc
      901 tttctgttaa cgattcaata gaagccatta atttgaaaga tatttgtaga gaagtcgcaa
      961 aagaattaaa agtttgtgaa cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag
     1021 aaaaagggga tattaatgcg cagattcttt tctcttataa agcagaacaa tactacaaag
     1081 gctataaaaa tcatgaacat gaatttatac ctgaagtaaa aaaggactgg aacgatcaat
     1141 tgaagcatga gatccagcaa caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac
     1201 aaggcagaca acaagaaagg gagtgaaatc gtcatgatga aaaccgttat tttagcagaa
     1261 aagccgtcac aagcgaaagc ttatgccgat agcttctcta aagccactcg aaaagacggg
     1321 tattttgaaa tccaagatcg attgtttcct ggtgaaactg tgatcacgta tggttttggt
     1381 catttagtcg aattagattc gcccgatatg tacgatgaaa attggaaaca atggtcatta
     1441 gaacatttgc ctattttccc tacacattat cactatcatg ttcctaaaga taagaaaaag
     1501 caattcaagg tggtgaaaca gcaacttcaa tcggccgaca ccattattat tgcgacagat
     1561 agcgatcgtg aaggggaact gatcgcttgg acgatcattc aacaagtagg cgccgatcaa
     1621 ggaaaaacct ttaaaagact ttggatcaac tccttagaaa aagaagccat ttatcaggga
     1681 ttccagcagt tacgagacgc aaaagagacc tatccaaaat ttgaagaagc acaagcacgt
     1741 cagatcgccg attggttaat tgggatgaac ggtagcccac tgtatagttt actcttgcag
     1801 caaaaaggca taccaggaag cttttcctta ggcagagtcc aaaccccaac gctttacatg
     1861 atttatcaat tgcaggaaaa gattagaaat ttcaagaaag aaccttattt tgaaggaaaa
     1921 tccgaagtaa tcgctcaaaa tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa
     1981 gcgacgcaag aagcatttga agattattta aaagaaaagg gggttcaatt gggaaaacag
     2041 ccaggcatga ttcgccaagt tgaaaccgag aaaaaaagcg cggcaagtcc acgtcttttt
     2101 tctctatcca gccttcagtc taaaatgaat cagctcatga aagctagtgc caaagacact
     2161 ttggaagcca tgcaaggctt gtatgaaggg aaatatctaa gctatccacg aacagacacc
     2221 ccttatatta cggaagggga gtatgcgtat ttgttggatc atttagatga atacaagcat
     2281 tttttaaagg ccgaatcaat cccaacccct attcatacgc cgaatagtcg gtatgtgaat
     2341 aacaagaaag tccaggaaca ttacgcgatc attcctacaa aaacggttat gacagcagcg
     2401 gcttttgaac aactaagtcc actgcagcag gccatttatg aacaagtgtt aaaaacaacg
     2461 gtggccatgt ttgcggaaaa atatacctat gaagaaacaa cgatcttgac gcaagtccag
     2521 cagcttcagt taaaggcaat cggaaaagta ccgatagatc taggttggaa gaagttattt
     2581 ggcaaagaga acgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa
     2641 ggagaaatgg tgacggtaga tctacaagtc cttgaaaaag aaaccaagcc accacagcct
     2701 tataccgaag gtacgttgat cacggcgatg aaaacagctg ggaaaaccgt tgatagtgaa
     2761 gaagcgcaat ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat
     2821 attattgaaa ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg
     2881 acgaataaag ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgag
//
LOCUS       DAZUMO010000150         2926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000150 DAZUMO010000000
VERSION     DAZUMO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.157"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..1193
                     /locus_tag="ACG8ER_002866"
     CDS             225..1193
                     /locus_tag="ACG8ER_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5051376.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1186..2217
                     /gene="vanA"
                     /locus_tag="ACG8ER_002867"
     CDS             1186..2217
                     /gene="vanA"
                     /locus_tag="ACG8ER_002867"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5051377.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2223..2831
                     /locus_tag="ACG8ER_002868"
     CDS             2223..2831
                     /locus_tag="ACG8ER_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS5051378.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          865 a          560 c          712 g          789 t
ORIGIN      
        1 gaggtcctaa gagatgtata taatttttta ggaaaatctc aaggttatct ttactttttc
       61 ttaggaaatt aacaatttaa tattaagaaa cggctcgttc ttacacggta gacttaatac
      121 cgtaagaacg agccgttttc gttcttcaga gaaagatttg acaagattac cattggcatc
      181 cccgttttat ttggtgcctt tcacagaaag ggttggtctt aattatgaat aacatcggca
      241 ttactgttta tggatgtgag caggatgagg cagatgcatt ccatgctctt tcgcctcgct
      301 ttggcgttat ggcaacgata attaacgcca acgtgtcgga atccaacgcc aaatccgcgc
      361 ctttcaatca atgtatcagt gtgggacata aatcagagat ttccgcctct attcttcttg
      421 cgctgaagag agccggtgtg aaatatattt ctacccgaag catcggctgc aatcatatag
      481 atacaactgc tgctaagaga atgggcatca ctgtcgacaa tgtggcgtac tcgccggata
      541 gcgttgccga ttatactatg atgctaattc ttatggcagt acgcaacgta aaatcgattg
      601 tgcgctctgt ggaaaaacat gatttcaggt tggacagcga ccgtggcaag gtactcagcg
      661 acatgacagt tggtgtggtg ggaacgggcc agataggcaa agcggttatt gagcggctgc
      721 gaggatttgg atgtaaagtg ttggcttata gtcgcagccg aagtatagag gtaaactatg
      781 taccgtttga tgagttgctg caaaatagcg atatcgttac gcttcatgtg ccgctcaata
      841 cggatacgca ctatattatc agccacgaac aaatacagag aatgaagcaa ggagcatttc
      901 ttatcaatac tgggcgcggt ccacttgtag atacctatga gttggttaaa gcattagaaa
      961 acgggaaact gggcggtgcc gcattggatg tattggaagg agaggaagag tttttctact
     1021 ctgattgcac ccaaaaacca attgataatc aatttttact taaacttcaa agaatgccta
     1081 acgtgataat cacaccgcat acggcctatt ataccgagca agcgttgcgt gataccgttg
     1141 aaaaaaccat taaaaactgt ttggattttg aaaggagaca ggagcatgaa tagaataaaa
     1201 gttgcaatac tgtttggggg ttgctcagag gagcatgacg tatcggtaaa atctgcaata
     1261 gagatagccg ctaacattaa taaagaaaaa tacgagccgt tatacattgg aattacgaaa
     1321 tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat gggaaaacga caattgctat
     1381 tcagctgtac tctcgccgga taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa
     1441 tatgaaatca accatgttga tgtagcattt tcagctttgc atggcaagtc aggtgaagat
     1501 ggatccatac aaggtctgtt tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa
     1561 agctcagcaa tttgtatgga caaatcgttg acatacatcg ttgcgaaaaa tgctgggata
     1621 gctactcccg ccttttgggt tattaataaa gatgataggc cggtggcagc tacgtttacc
     1681 tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat
     1741 agcgcggacg aattggacta cgcaattgaa tcggcaagac aatatgacag caaaatctta
     1801 attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg
     1861 ttagttgttg gcgaggtgga ccaaatcagg ctgcagtacg gaatctttcg tattcatcag
     1921 gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa ccgttcccgc agacctttca
     1981 gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt
     2041 agaggtctag cccgtgtgga tatgttttta caagataacg gccgcattgt actgaacgaa
     2101 gtcaatactc tgcccggttt cacgtcatac agtcgttatc cccgtatgat ggccgctgca
     2161 ggtattgcac ttcccgaact gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa
     2221 gcatggaaat aggatttact tttttagatg aaatagtaca cggtgttcgt tgggacgcta
     2281 aatatgccac ttgggataat ttcaccggaa aaccggttga cggttatgaa gtaaatcgca
     2341 ttgtagggac atacgagttg gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc
     2401 aagggtacgg attgcttcta tgggacggtt accgtcctaa gcgtgctgta aactgtttta
     2461 tgcaatgggc tgcacagccg gaaaataacc tgacaaagga aagttattat cccaatattg
     2521 accgaactga gatgatttca aaaggatacg tggcttcaaa atcaagccat agccgcggca
     2581 gtgccattga tcttacgctt tatcgattag acacgggtga gcttgtacca atggggagcc
     2641 gatttgattt tatggatgaa cgctctcatc atgcggcaaa tggaatatca tgcaatgaag
     2701 cgcaaaatcg cagacgtttg cgctccatca tggaaaacag tgggtttgaa gcatatagcc
     2761 tcgaatggtg gcactatgta ttaagagacg aaccataccc caatagctat tttgatttcc
     2821 ccgttaaata aacttttaac cgttgcacgg acaaactata taagctaact ctttcggcag
     2881 gaaacccgac gtatgtaact ggttcttagg gaatttatat atagta
//
LOCUS       DAZUMO010000151         2833 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000151 DAZUMO010000000
VERSION     DAZUMO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.158"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1392
                     /locus_tag="ACG8ER_002869"
     CDS             <1..1392
                     /locus_tag="ACG8ER_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5051379.1"
                     /translation="KVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYE
                     GVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPAL
                     PEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPE
                     GQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSD
                     EVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVD
                     VSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNA
                     EVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNK
                     KDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTL
                     GLLGYKNRKKENE"
     gene            1526..>2833
                     /locus_tag="ACG8ER_002870"
     CDS             1526..>2833
                     /locus_tag="ACG8ER_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5051380.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT         1108 a          387 c          554 g          784 t
ORIGIN      
        1 aaagtagaag ttgggggcac agtagatcta acagataatg ttactaactt gccagcgtta
       61 ccagaaggaa caacagtaac agatgttact cccggcggta caattgatac caacacacca
      121 ggtaattacg aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa
      181 gttccagtag aagtgacaga caatcgttct gacgccgata aatatgaacc aacagtagaa
      241 ggcgaaaaag tagaaattgg aggcacagta gatttaacag ataatgttac taacttgcca
      301 gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac
      361 acaccaggca attatgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg
      421 gtgaaagttc cagtagaagt gacagacaat cgttctaatg cagataaata caatcctgaa
      481 ggacaaaaag tgactacaga cttaaataaa gagccagatg catctgaggg aattaagaac
      541 aaagaagatt taccaaaagg aactacttat gcttggaaag agaaagtaga tgttagcaca
      601 gctggaaata aaaaaggtac tattgtagtg acatatccag atggatcatc tgatgaagtt
      661 gaagtagacg ttacagtgac agatactcgt tccgatgcag ataaatataa tcctgaagga
      721 caaaaagtga ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa
      781 gaagatctac caaaaggaac tacttatact tggaaagaaa aagtagatgt tagtacagct
      841 ggaaacaaaa aaggtactgt agtagtaaca tatccagatg gatctaaaga agaagtagaa
      901 gttactattt ctgtagtaga taaaaaagca ccgaataaac ctcaagttga tcctattaca
      961 gagggtgacc aaattgttac tggtaaaact gaaccaaatg cagaggtgac agttacatta
     1021 ccagacggaa gtcaacacca tgggacagct gataaaaatg gtaattttac agttaaagtt
     1081 cctaaattag aggcagggac aaaagtgata gtaactgcaa ctgatgaatc tggtaatact
     1141 agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt
     1201 aaaggtagta agactgacaa tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa
     1261 agccagtcct caaaagtttt tcctaagacg ggagagagtg acagtaatat ctttactatt
     1321 agcggtggtt taatactgtt aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa
     1381 gagaatgaat agaaaatcaa tttcttaagt cttctctgag aagaggaaga atctttttct
     1441 tcctcttttt gcttttttat gatgggaaat aagtgttagt taaagatgaa tatacttttt
     1501 atagaaagga aaagaatgta attaagtgga aaaaatagta attattggtt caaaccattc
     1561 agggctagcc gcagctcgat ttctaaactt aagcgattca aattatgaag ttattctaat
     1621 cgataaaaaa tcagaaatta gttacttgga atgtgggaca tcgttattta taaaaggaga
     1681 agtaagtaat ttagaagatc ttttttatca aaatagaatg acactaaatt ctgagataaa
     1741 aaagttattt ctagagacag aagttgacaa aattgatatt caaaataaaa agattagttg
     1801 tgtaaaaaaa gatggtacaa gattctctca agaatatgac aaagttatat tagctacagg
     1861 ttcaaagcaa attatgctag atataccggg gaataattta gctaatattt tttcgataaa
     1921 gaattttcca aatgcaaaag agatttttaa agctttggat gatagtacta ttaaaaaagt
     1981 aggaattatt ggagcaggat atattggagt tgaaattgct gaagccataa gaaaaagagg
     2041 gaaagaagta tatcttttcg atgttgcaga tagagtgttg tcgacttatt acgaccgtag
     2101 tttttcagat aaagttgaag aaatactatc aaaaaatgga atacatcttt gtctatcaga
     2161 aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa atagtcacta caagtggtac
     2221 ccaatactct atggatatgg tcgtacaagc tataggattt ctatctaata gtccaattgg
     2281 ggaaaaggaa ctcttgagag atgttagtgg agcatatcta actaatgaat accagcaaac
     2341 aaatataaaa gatatatatg ctattgggga ttgtgctaca acttttttta cgtccattaa
     2401 tagaagtagt gttgattttt ctatttctaa tgctctaaga acagcttata ttgcaactca
     2461 acatattaac aatcaagatt ttacaccttc tggttcccaa tttacaaatg gcttttctat
     2521 atttaattac aatttttacg cagcaggttt gaatttaaaa acagcacgat tattaagtga
     2581 gtctatagat tatatagaaa tagaagattt cattagacct gcttttttga gaaaaaatag
     2641 tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag agaatagtag gtgtacaact
     2701 ttgtagtcaa gaagatttat ctggactatt atcaatgttt tctcttgcta tagaagaaca
     2761 aattacatta gacaaattga aaatgttaga ttatttattt catcctagtt ttagccaacc
     2821 ctataatttt att
//
LOCUS       DAZUMO010000152         2815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000152 DAZUMO010000000
VERSION     DAZUMO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.159"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..457)
                     /locus_tag="ACG8ER_002871"
     CDS             complement(80..457)
                     /locus_tag="ACG8ER_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051381.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(757..1638)
                     /gene="galU"
                     /locus_tag="ACG8ER_002872"
     CDS             complement(757..1638)
                     /gene="galU"
                     /locus_tag="ACG8ER_002872"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5051382.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1681..2700)
                     /locus_tag="ACG8ER_002873"
     CDS             complement(1681..2700)
                     /locus_tag="ACG8ER_002873"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5051383.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          763 a          634 c          427 g          991 t
ORIGIN      
        1 ttttccacca attaaataaa tctctcatct caatagttta tccattattt tatctttttt
       61 tgcgtatacc tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat
      121 ctgtactaga aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca
      181 tatatgggat ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt
      241 cccaactacc taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc
      301 agataaaaat cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa
      361 taagcaatag tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat
      421 caagcagcga ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat
      481 aattaaaaaa ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct
      541 tatgtaatca ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt
      601 ctgaatctat tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag
      661 agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa
      721 atagcctttc attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt
      781 actcaactgg ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata
      841 ttcaatattt gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa
      901 ctctctcgca tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat
      961 ctcgtttcct gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag
     1021 ataacgtccg ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa
     1081 cccttttact ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt
     1141 agatgtttca ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata
     1201 atccatcatc aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat
     1261 gacgacaaac ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc
     1321 tcttggatga gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc
     1381 gagtaattct gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa
     1441 gtgatcttca atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga
     1501 atccaatgct tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc
     1561 ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac
     1621 tgcttttttg accttcactt gctgttcctc ctaaacgacg attaactatt gatcatctat
     1681 ttaacgaaga gaaaattcat tctccatttt tccgtcacgt aacatgattt cttttgctgc
     1741 ttttttgata tcttctccat tatacaaaac attatagatt gtttgagtaa ttggcatctc
     1801 aacacctagc tgttgagcta gctctactgc tgcttttgtc gttgccacac cctcaacgac
     1861 cattcccatg ttttctagaa cttcttccag cttatgtccc tgtcctaaca agttaccggc
     1921 acgccaattg cgagaatgaa cacttgtaca agtaacgatc aagtccccca caccactcaa
     1981 accgataaat gtcagaggat tggcacccat cgcgacacca agacggctga tttctgccaa
     2041 tcctcgagtc atgattgctg cttttgcatc atctccgaat cctaatccat ggatagcgcc
     2101 ggcaccaatt gcaatgatat ttttcagtgc tgcaccagtt tctacgccaa tcacatcatt
     2161 atttgtataa atcctgaaat aatcattcat gaacagtttt tgtacatagg ctgctgcttt
     2221 ttgatcaata cttgcagctg taattgttgt gatatcatgt actgccactt cctcagcatg
     2281 gctaggacca gataatacaa caattgcctg tcttttttct tctggaattt cttcagccaa
     2341 aacttctgag attcgtttat gcgtatcttg ttctagtcct ttacttgcat gtataatgat
     2401 tggcttagta ttcattttac caataagttc ctgtgcgact gaacgaatcg cttttgttgg
     2461 aacaacaaat aaaatagcat ctgcgttatc cactgcttct gccaattctt tatatccgat
     2521 aatcgattct gggatcgtca aatcaggtaa aaaatgctga tttgtatgat acttattgat
     2581 ttcatcaatc tgttcaggta catttcccca aatacggaca tcatgcccgt tttctgccaa
     2641 tgtttgtgct aaagcagtgc cccaagatcc aggaccaaga actgcgattt tttgtttcat
     2701 ggatattcct ccaaataaat aattgataat ttccattata gtatacattt cttaaagttg
     2761 ggactaattt ccattgtttt taagtgtttg tttacttctt ttatcgtttt tactt
//
LOCUS       DAZUMO010000153         2491 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000153 DAZUMO010000000
VERSION     DAZUMO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.160"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1335
                     /locus_tag="ACG8ER_002874"
     CDS             <1..1335
                     /locus_tag="ACG8ER_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051384.1"
                     /translation="IDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1322..>2491
                     /locus_tag="ACG8ER_002875"
     CDS             1322..>2491
                     /locus_tag="ACG8ER_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS5051385.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKN"
BASE COUNT          831 a          377 c          420 g          863 t
ORIGIN      
        1 atagatgctg cttttttatt tagttgttta tatacttttg caacttatcg aacagttgat
       61 atctattatc gatccgcaat tgcagaagca atcgctctta cgattatccc agctgtttta
      121 tattatgctt atggattgat attcaataga gaaaaaaaag tcatcgcttt atctgtcagt
      181 ataagtctac ttgtgtatac tcatgtctta actacactat tgtgtagtgc attgattgct
      241 tgtatgatta tagttcgttt gtttttaagc aagcaaataa aaaacgatat aaaatttgtg
      301 gtcgtgaatt tagtaaaggc tgtttttttg acttgtgcat taactagcta tttttggtat
      361 cctatgttgc agcagatgaa ttatcaagaa atacacagtc ccttcaaaac agatttgcag
      421 ctagaagcac tttctatttc agatagtatc attcaagcgt taaataacga tattacaaca
      481 tacactatgg ggttattagg attggttgcg ttgatttttc ctttattcat actgaaagaa
      541 atggagaaaa aagaaaaaat catctatgtt ggcgtagttt ctacatgggt atgcgtaact
      601 aatttatttc catggttttt gtttcaaaaa acaccgataa gcttactcca gtttccttgg
      661 cgtatcttag gtatccaaat ctttttttct tcaatgatca taacgcttgt ttttatgaaa
      721 aagactatga ataaaaaata ttcatggtta atcattggcg ctactgtggt gtttctactc
      781 gtcacgtcta tagcagcaaa agaaaattac gcaaaagtta tccaatcaaa acacggtcat
      841 attgttatca atgaagaaac actcccacgt tatacaacat cagatatggg aggattatac
      901 gattacgccc caacagaagc gataaaagaa aggcctcact tagaaaaaca tgaagtaaaa
      961 gtaggagaat cgtgggaaaa agggaagtat aaagctgcag ataataacat tacttacaca
     1021 gtggcaagca acaaaaagca aaaagtgaca gtaccagtat atgcttattt aggtacacag
     1081 gtaaaagtaa acggtaaagt cgtaaatgac tcttataaaa aacatggctt agtgaatgtg
     1141 gatgtaaatg ctggagaaaa taagatagaa attacaacaa aatatactcc aactgctctc
     1201 atggctttat ggatcagtat tctgacttat tgtttagtgg tgtattcaag ttttaaacga
     1261 tttgcaccta ataagttgaa agattctcga tatgtgacta aaaaggagat aaacaagaag
     1321 aatgaaaata aataaaaaag tagtaatatt gagtggatta attatagtta gtagtatata
     1381 ttctttcatc tttttcaaaa atacacagag tatttatacc actggagatc tttcttttca
     1441 cttatctcga atcaaggggt tatcgactat ttttaccagt ccgattaatt atgagacatt
     1501 caattatact ggatatggtg taaattattt ttatcccttt ctgacttttt ttccagctgt
     1561 aatgttatat tggatgacaa aaaatttgat tgtaagctat attatttacg tttggttatt
     1621 gaacttgtgt acaacactcg tagcttatca ttatggcgag cggtttttaa aacaaaaaaa
     1681 agcggcattt ttatttagtt gtctctatat tttctctgct tatcggacag tagacatcta
     1741 ctatcgttcc gcaattgccg aagctatcgc aataacactt gttatccctg tattatttta
     1801 tgcttatcaa atcatctctg gaaaagaaga aaagtatccg tctgtaaagc tagcattgag
     1861 tatgagtttg ctagtgtaca gccatgtact atcaacatta atgagtacag cattaattat
     1921 catttttatt tttattcgtt tggtttctaa aggtttcaag aatgcagatt ttattgctat
     1981 atttaaaaaa ttatttagtg cggcaggaat gacattggtt ctgacgagtg ctttttggta
     2041 tccgatgttt gaacaaatgc tctaccaaaa aatcaacaaa cctagcgtga caaatttata
     2101 tgctcatgca tctaatgtgt tcgatagtct gactgaagct atgaacaacg acttaactac
     2161 ttactcaatg ggactagttg gtctgttatc tttatgtatt cccctgattt tatttaaaaa
     2221 attaacaaac atagaaaaaa agatttatta tggtacgtgt ttgacttggc tagctacaac
     2281 tagtttggtg ccatggtatc ttctgcaaaa tacgccagcc aaattgttac aatttccttg
     2341 gcggatctta agcttgcaaa taatattctc ttctctgata ttaacgatga tatttttcaa
     2401 aaatagaaga tacaataaga caagagaact attttattta tttatgactg ttttgttatt
     2461 aatcacttta actgtttcta gtaaaaaaaa t
//
LOCUS       DAZUMO010000154         2427 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000154 DAZUMO010000000
VERSION     DAZUMO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.161"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..292)
                     /locus_tag="ACG8ER_002876"
     CDS             complement(8..292)
                     /locus_tag="ACG8ER_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051386.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(992..1087)
                     /locus_tag="ACG8ER_002877"
     CDS             complement(992..1087)
                     /locus_tag="ACG8ER_002877"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5051387.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            1740..>2427
                     /locus_tag="ACG8ER_002878"
     CDS             1740..>2427
                     /locus_tag="ACG8ER_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5051388.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLP"
BASE COUNT          791 a          389 c          454 g          793 t
ORIGIN      
        1 ctcatggtta tatcattcct ccgataagca tttcaatgat ttgattattt gtgtagacat
       61 actcgcttaa tatttgagta ttttctgttt ggatagcaat aaatttatcg agcaataaat
      121 cgaattggtc ttttttgtaa gtcgtaagtt gacggtcaaa atcactttcg attaaaactt
      181 ttagatattt atttcgactc catttttctc ctgttttatt cgtgagttct tggcaacgtt
      241 tgtctattgt tgcaacaaac tttggatcaa tattccgaac taaaatgtcc ataaaaaatc
      301 tccttttgca attatgtata atttatgtat accatacaaa tactatattt gtatagctgt
      361 tttttgccaa aatgctatac ataagctacc cacgatggcg aaaaagtgat atcaaatttt
      421 tttctgagaa acgtgatagg cacagcctat ctacccactt tttttagaaa gtgggtacac
      481 gtttccttat gctctttaaa attcgggggg gagtgctgca cgtgtaatcg ataaccaaat
      541 ttaacagacg aaaaattgtt ggaatataaa aatatgacac ccgaaagaaa acgatcgttg
      601 gaaaaattaa tgcaactagt gaacgaagac ggcggaattt cagacgcagc ttttgacgca
      661 tggagcgaat ttcatggaga atataattaa gtgggtttaa atggctctaa aatcgcttct
      721 aagagttttt tagcttggac caatgaaata caccaaaaac taaataaaac gtcttaaatc
      781 gaaaaatacg agctctcaga gcgactttat ttgtaaatgt tgcatataag aaataattac
      841 ggattttaga cttttgtagg atatttttga aataaatatt tatttctgtt ataatttgat
      901 tgcaaataag tgaacacgaa acacacgaaa tccccagaga ggtcggattc tctggggctt
      961 tttttgtggg ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt
     1021 aactgaacaa agcgcttaaa agcccaacaa gaagggagca aataaagtta gtgaacacga
     1081 aacacacctc cttccagtcg caagactgtc ggataggcga caatcaatta tacaataaaa
     1141 aagcaaaaat aagcctaagt tttttattgc cttggggttg ttagggcttg ccctgaccgc
     1201 ctgtaaggcg ctttagaacg aaggacatag tttttatcga atcttgtctt agaacggctt
     1261 agacgagctc tcaggacgcc acaaaggagt ccctaatgaa ttatttttgg cgaaggagcc
     1321 taaaggcgac tgctgcaagt aaaatgtgag agcgtagcga tcactccttc ttacagcgac
     1381 catagggagc tagattggca gtttaatatc atgcgcgaac ctgtttattt taaaaagtgg
     1441 gtgatttttt ggcgggaaat tttgctcgcg cgcgcgcgtt tattttattt attttttaaa
     1501 gattttttag aacccttttt agggggcagt tgagccttac tctcccaacg gatacagaag
     1561 ggtactaaca actttttatc tgagtactaa caacttttta tctgagtact aacaactttt
     1621 tatctgagta ctaacaactt tttatctgag tactaatatc cttttgtata ataacaacca
     1681 acctatttac gagttggttg ttaaaggtta ttatttattt gtaagggggg aagaaaggaa
     1741 tggatgaaaa aacggaatta acagtcaaat atcaaaatga attgaattta gtaccgctga
     1801 aaaatttcaa tgctaagcag atggatttat tttttgcatt atgcgctcgt atgaaagatc
     1861 gtggtttgaa aaaagttaga ttttctttcg aggaattaaa agagttaagt gattataaga
     1921 tgactgctac aaaagccttt gtcggtgatt tagaaaagct ctataaagat atgcttaatc
     1981 ttacttaccg tactgaagat gaggattcta ttcgctactt tgttttgttt actggttttg
     2041 aaatcaataa aacaaaaaag tttgttgaag tcagtgttaa tccggaccta gaatatatta
     2101 ttaatggttt aacagctgaa tttagtagat ttgaattatc tgctttcacg tcgttgcgtt
     2161 ctacgtatgc aaaaactcta ttccgactgc ttatgcagta ccgttctact ggttattatg
     2221 tagttcgtat tgaagagttt aggagcttaa tggatatccc agaatactat caaatgggta
     2281 acattgatca aaaagtatta aatcctgcat tacgagaact aaaaaaatac ttcaatgatc
     2341 ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat cgccaaatta gaatttactt
     2401 tttcaagcct aacgtcagat ttgccaa
//
LOCUS       DAZUMO010000155         2418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000155 DAZUMO010000000
VERSION     DAZUMO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.162"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG8ER_002879"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG8ER_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG8ER_002880"
     CDS             1320..1778
                     /locus_tag="ACG8ER_002880"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS5051389.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACG8ER_002881"
     CDS             1937..2407
                     /locus_tag="ACG8ER_002881"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS5051390.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          699 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaa
//
LOCUS       DAZUMO010000156         2395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000156 DAZUMO010000000
VERSION     DAZUMO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.163"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8ER_002882"
     CDS             316..570
                     /locus_tag="ACG8ER_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5051391.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8ER_002883"
     CDS             571..888
                     /locus_tag="ACG8ER_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5051392.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG8ER_002884"
     CDS             1447..2382
                     /locus_tag="ACG8ER_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051393.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZUMO010000157         2367 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000157 DAZUMO010000000
VERSION     DAZUMO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.164"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..2220
                     /locus_tag="ACG8ER_002885"
     CDS             67..2220
                     /locus_tag="ACG8ER_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS5051394.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          811 a          417 c          528 g          611 t
ORIGIN      
        1 ttctttttta ttaagaagtt tttgggtaca atgaagcatg aatgtaaata ggcggaagga
       61 taggaaatga tgaaagattt tatgaaaaaa atattaaata cttcttggtt agaaaaaata
      121 agaaaaggtg cacaagaacc taagaacaaa acgtttcgcc ggtcccatgt tccttttcgc
      181 ttaaattttc tttttttcat tatatttacg ttatttgttg cgttgattgc tagattgggt
      241 tatttgcaga tcgtgaacgg cgaagagatg gaggcgcgag tgaagtctac ttctacgatt
      301 acggtccaag aaagtactcc tcgcggaatg atctacgact ccacaggaaa agctttagtt
      361 acgaataaag cgaatcaggc catcacatat acgagaggaa cacaagttac ggctaaagat
      421 ttgcttgaaa tcgcaacgaa gctgaactcg ctgatcgatg ttccggtaga tgaaaatctg
      481 acagatagag ataaaaaaga tttttggctg gctgatccca aacatttgaa agaagccaca
      541 aaaagacttt cggcaaaaga aaagcaatta gggaacagcg aacaatatag tgcgatggtc
      601 aataaagtca cagctgatga gattaattta aacgaggagc aattaaaaat tgctaccatc
      661 tttaaacgga tgaatagtgc atatgaatta aacactgtct atatcaaaaa ctctggtgtt
      721 actgatcaag agttagctgt tgtggcagaa cgcgcttctg aacttccagg agtttcgact
      781 ggcacagact ggacacgaga atataatgca gcaagttcac tgaaaagtat tcttggcagt
      841 gtgacgactg aaaagcaagg acttccagca gatgaagcag agaaatacct agcaaaaggt
      901 tattctcgga atgaccgtgt aggacaaagt tatttggaaa aacaatatga agatgtcctg
      961 caaggaacca aaacccaata tgaagtttcc cttgataatg aaggaaatgt ttcgaatcaa
     1021 aaagaaatct tctctgggga aaaaggttct aatttaatgt tatctatgaa tgcagaattc
     1081 caatcaaaag tagaagaaat tttgaaaagg aattatcaaa cattgatcaa caacggcaaa
     1141 gcacagtatt cgcctggtgc ttatgcagtg gctatgaatc ctcagacagg agaagtgcta
     1201 gcaatgactg gtttcagtca tgaacaaggt tccaaagaaa tcaccgagaa tgctttaggc
     1261 acgattacta gcgcctttga tccagggtct gtagtcaagg ctggtacttt gacagcaggc
     1321 tgggcaagta acgcgatcag cggaaatcaa gttttgattg atgagccgat tcgtctgcaa
     1381 ggtgcttcag aaaaaagctc tgtctttaat cgaagcggac aagttgctct tgatgcagtc
     1441 aaagcatcgg aattgtcttc taatacgtat atgatcaaag tggcgttaaa gatgttagga
     1501 ctagactata cacctggaat gggactacct agtttagatg aagaagctaa agcttatcag
     1561 caattgagag actctttcaa agagtttggt cttggtacaa cgactgggat cgatttgccg
     1621 aatgaatctc ctgggatctc acgttctgtt gattatatga agaagttcaa tgcggataat
     1681 ggcaaagaat ggtatgctcc aggtaacttt accgatttgg catttggaca gtttgacaca
     1741 tatacaccaa tccaattagc tcaatatgct tcaacagtcg ctaatggcgg gaaacgagta
     1801 cagccgcacc tagtcaaagc gatctatggc aatgacgaaa atggaaactt gggtgaagtg
     1861 aagaaagaaa ttggcacaac agtagaaaat acggtcaata tttctgcaga aaatatggcg
     1921 atcctccgtg aaggattcca ccaagttgtc catggtaccg atccttacac aacagccaaa
     1981 ccattagcca gtgcgaaaat ggacctttcc gcaaaaacag ggactgcaga aacggttgcg
     2041 gaaggtcatc cagatattgc gacagtcaat agtaatatcg tggcatacgg accaacagac
     2101 aatccggaaa ttgccatcag tgttgtcttg ccgaacttat tagacgaaca agaccacata
     2161 aacttgacga ttgctaaaga aataatggac gcgtattatg atatgttcat ggcaaaataa
     2221 aaaagaggtc gtgaaaaagc cgcttagttt cgagtatgaa gaaaaatttt gtgaaaatag
     2281 cttctcacat ttttgcttaa atttgacttc ataccgagaa actggctttt gaacaccgtt
     2341 tactcggcaa aaaaggttgt gacaaaa
//
LOCUS       DAZUMO010000158         2317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000158 DAZUMO010000000
VERSION     DAZUMO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.165"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..1764
                     /locus_tag="ACG8ER_002886"
     CDS             151..1764
                     /locus_tag="ACG8ER_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5051395.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNNYTSARILAGLGIKAILIEE"
BASE COUNT          731 a          408 c          448 g          730 t
ORIGIN      
        1 cttaaaatag aaaaaaattt ttaggtttta tccacaaatt atttctctat attttctgtc
       61 tattcaaaat aaacaataat tttccggcac aattatttgc tgttttttac cataacatag
      121 tacgatcaac gtagaaaagg agatgttttt atgaaagttg ttgtcattgg cggtgtagct
      181 ggcggtcctt catttgccac tcgtttccgt cgattgaatg aagcgcacga aatcattatc
      241 tatgaacgcg gagaaaatat ttcttatgca agttgtgctt tgccttatta tttaggaggt
      301 gtgatcactg accgtgactc gctgatcgaa cgtacaccag aaatattgaa aacaaaaaac
      361 aacatcgatg tctttactaa acacgaagta acagcaatcg atccttctac taagcgatta
      421 acagttaaag acctatccac aaatgaagaa ataaaaacag attacgataa gttgatcatc
      481 tcttctggtg ctagaccaga ttatccggat attcccggag tttttgaagc agaaaacggc
      541 tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat cgttccttga agaaaaaaat
      601 ccacaacatg tcgtcattct tggtgcaggt gttatgggtc tggaattagc tgagaatttc
      661 aagcatcgcg gcttagacgt gactttaatc gatcaattgc cacaagtcgc tttcccttat
      721 gatccagaaa ttgctaattt agtttatgac aaattgctga aagaaggatt agccgttcat
      781 ttagaaacaa gagttactga gatccgtgat aaaggtcgag aaatagtatt atcagatggc
      841 tccgtccttt ctgctgatat gctaattttt gctgttggtg tttctccgaa gaatgaagtg
      901 gtgaaagcaa ccggcataca attatcagat acaggtcaga tcattgttga tgaccagtta
      961 caaaccaatc tgccggacat ctatgcgatt ggcgatatta tcgaaacgac tagtgtagtg
     1021 actggtcagc cgatccaaag tatgctttcc agtgcggcca atcgtcaagg acatatgctg
     1081 gcagatattt taaatggtac gcctatgcgc tatcgcggat atattggtgc aggtgtcgca
     1141 aaaatctttg atcatacagc aagttatgcg ggaatgacag aacatgaatt aaaagcatca
     1201 ggcataacaa attataaaac tgtttttatc actccctttg accatgccta tttctatcca
     1261 gaagctacaa gattaaatct aaagctgatt tttgatgcag atagcggtcg tattttaggt
     1321 ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg gagaactttc tgtagcaatc
     1381 accggaaact tgacagtctt tgatttgcct gatttggagt tgccttactc tccaccgtat
     1441 tctactaccc gtgacccgtt gaatatagct ggttatgtcg caatcaatca aatgacgaat
     1501 atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg atttgaaaga agcgttcttt
     1561 ttagatatac gtgaacctaa taaagcacct tctggcagta tctcggcaac aaaaaatatt
     1621 ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa aagataaaaa agtttacatt
     1681 actttcagaa gaggattgaa taattatact tctgcccgaa tcttggcagg tttgggtatc
     1741 aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg ggtagccgcg gtctaggagt
     1801 tatttttact tgaggagtaa aaatgaaaaa gtatataggg ttgttggttg gtatgtatat
     1861 agaatagaag ataaatgtga agaaattgtg cattatttct tcacatttat taggttatag
     1921 tttaagaaag tttaaacatt gcgcaattta attattttag ctgtttcttt tcataaaaaa
     1981 acatgttgtg actctgagac aaatcatagt ccatagtgat gtctctagag taactacgcc
     2041 taatattacg aggcatcata ttaatctatc agcactagta cctaaactag catccctttc
     2101 gaatattttt tcttatataa taaagtttgc tttcactcct ctaatctcac caaatcgact
     2161 ggcttgcgtt gtgtgatact aggaactgat tggtttagaa cgacttttct attgtaatca
     2221 tatttttcgc catcctcgtt gtcttctaat ccttcaatga atgatcccta atgtgtgtat
     2281 taaataattt aatttttttg ttcctattta tttaaca
//
LOCUS       DAZUMO010000159         2317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000159 DAZUMO010000000
VERSION     DAZUMO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.166"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..350
                     /locus_tag="ACG8ER_002887"
     CDS             <1..350
                     /locus_tag="ACG8ER_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5051396.1"
                     /translation="EAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFE
                     DAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDE
                     WIYSSRKYINFDK"
     gene            407..799
                     /locus_tag="ACG8ER_002888"
     CDS             407..799
                     /locus_tag="ACG8ER_002888"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5051397.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            800..2239
                     /locus_tag="ACG8ER_002889"
                     /note="aph(2'')-Ia"
     CDS             800..2239
                     /locus_tag="ACG8ER_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS5051398.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRAYKD"
BASE COUNT          951 a          209 c          400 g          757 t
ORIGIN      
        1 gagaagctgt taaaggaatt tttaagttca cagatattaa cttagcgcgt gaggctaaaa
       61 atcgattgat tcatgattat atcgatcaac caaaatattc aaaagcttgc gcatcattgg
      121 atgatggatt cgaagacgcc tttcaatata ccgtacaagg aaattcccac aatcgactaa
      181 agagtaccaa tctaattgaa cgactgaatc aagaagtacg cagaagagaa aagattattc
      241 gcatcttccc caatcaaaca tcagccaatc gcttaattgg agccgttctt atggacctac
      301 atgatgaatg gatttattct tcaagaaaat acatcaattt tgataagtag aaatggtaaa
      361 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
      421 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      481 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      541 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      601 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      661 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      721 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      781 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      841 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      901 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      961 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
     1021 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
     1081 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
     1141 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
     1201 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
     1261 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
     1321 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1381 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1441 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1501 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1561 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1621 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1681 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1741 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1801 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1861 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1921 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1981 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     2041 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     2101 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     2161 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     2221 aagggcttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     2281 taaatagttt aaaaaaatga aaaataataa aggaagt
//
LOCUS       DAZUMO010000160         2283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000160 DAZUMO010000000
VERSION     DAZUMO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.167"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8ER_002890"
     CDS             complement(<1..300)
                     /locus_tag="ACG8ER_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5051399.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8ER_002891"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8ER_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8ER_002892"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8ER_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051400.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="ACG8ER_002893"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="ACG8ER_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          652 a          433 c          333 g          865 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tac
//
LOCUS       DAZUMO010000161         2260 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000161 DAZUMO010000000
VERSION     DAZUMO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.168"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACG8ER_002894"
     CDS             complement(365..1657)
                     /locus_tag="ACG8ER_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5051401.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACG8ER_002895"
     CDS             complement(1931..2191)
                     /locus_tag="ACG8ER_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051402.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          746 a          430 c          343 g          741 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa
//
LOCUS       DAZUMO010000162         2132 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000162 DAZUMO010000000
VERSION     DAZUMO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.170"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACG8ER_002896"
     CDS             complement(51..1598)
                     /locus_tag="ACG8ER_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5051403.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8ER_002897"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8ER_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5051404.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          607 a          443 c          378 g          704 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tc
//
LOCUS       DAZUMO010000163         2116 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000163 DAZUMO010000000
VERSION     DAZUMO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.171"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..506
                     /locus_tag="ACG8ER_002898"
     CDS             3..506
                     /locus_tag="ACG8ER_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051405.1"
                     /translation="MNFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDS
                     GLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNR
                     KIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNP
                     KTYDILA"
     gene            complement(567..932)
                     /locus_tag="ACG8ER_002899"
     CDS             complement(567..932)
                     /locus_tag="ACG8ER_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS5051406.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1008..1253)
                     /locus_tag="ACG8ER_002900"
     CDS             complement(1008..1253)
                     /locus_tag="ACG8ER_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5051407.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1323..1577)
                     /locus_tag="ACG8ER_002901"
     CDS             complement(1323..1577)
                     /locus_tag="ACG8ER_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051408.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1741..2064)
                     /locus_tag="ACG8ER_002902"
     CDS             complement(1741..2064)
                     /locus_tag="ACG8ER_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051409.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          791 a          361 c          370 g          594 t
ORIGIN      
        1 ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg
       61 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
      121 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
      181 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
      241 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
      301 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
      361 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
      421 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
      481 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
      541 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
      601 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
      661 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
      721 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
      781 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
      841 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
      901 tttgaaatca atttttcatg ccataatcgc atagaaatgt ctcctttata tatagattgt
      961 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
     1021 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     1081 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     1141 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     1201 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     1261 cctttcatta tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt
     1321 actcaatgtt ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc
     1381 agtcctgata attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact
     1441 ttccacataa agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc
     1501 caattcataa gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact
     1561 ctcaccaacg taatcatgta acggactcct caacgattca tataataaaa gataacatgt
     1621 tattgcatac atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa
     1681 tgaaaaagca gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat
     1741 ctatcgagaa aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac
     1801 gatcgcattc actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac
     1861 aaatactttt gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag
     1921 aagcaaaagg gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg
     1981 ataatttaat tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa
     2041 tggcaaaata atcactccta acattgagaa taaataaaag aataacattt tttctaaaat
     2101 aagcaaataa aaatta
//
LOCUS       DAZUMO010000164         2094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000164 DAZUMO010000000
VERSION     DAZUMO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.172"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..189)
                     /locus_tag="ACG8ER_002903"
     CDS             complement(<1..189)
                     /locus_tag="ACG8ER_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051410.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTK"
     gene            complement(190..1047)
                     /locus_tag="ACG8ER_002904"
     CDS             complement(190..1047)
                     /locus_tag="ACG8ER_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5051411.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1333..1620)
                     /locus_tag="ACG8ER_002905"
     CDS             complement(1333..1620)
                     /locus_tag="ACG8ER_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5051412.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(1610..1939)
                     /locus_tag="ACG8ER_002906"
     CDS             complement(1610..1939)
                     /locus_tag="ACG8ER_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5051413.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2016..>2094)
                     /locus_tag="ACG8ER_002907"
     CDS             complement(2016..>2094)
                     /locus_tag="ACG8ER_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5051414.1"
                     /translation="RVVYKVDEDEHVVEIYSARTHYESQ"
BASE COUNT          619 a          419 c          278 g          778 t
ORIGIN      
        1 ttttgttctt cctctactct gtctttcaac attggaggaa gtttttattt cttgaacttg
       61 gtgcacttct aattgcttat tttcagatgg tgtaattgct tgctgctttt tctttgcttc
      121 tgcttctttt tttattttgg cgagtccttt gttgttccca aaaatatcaa gtcctttgat
      181 gtcagccaat tactccacct ccgcatacca attctcgtgt tcttccaatt ctttcaaaat
      241 actgataaat aattcgtggc ttttgcgatc ccattgtttt aagagacctt tctttgtctt
      301 ctccatcgta atacctgatc cgtcgtatcg ttttaagcgc gcatttttga gtacaacatt
      361 tttaatcaaa tgttccccat aaatctcttg ggcttgttta tacatttcct gatctactgc
      421 gtcaccgctt tccaacatga aaggaataac accagcaaca tataaatttg tatcaaattc
      481 tttcacaaaa aagtccatat actcaagata ttgcatcgca tttctcatcg attttacttg
      541 ggtctgtaag acgacgattg cccaatccgc aatgtacatc gcggatttac tatatacaga
      601 aatagaaggt gggacatcta aatagatgac gtcaaactca tcaaataatg gttctagctt
      661 ttctttcata tatttgaatt gggataatga atcattatat tggtctttga tccaatcatc
      721 atacatttca aaggacatgt ctgctggaag tatatagagg ttattgatta ctttatagac
      781 agcctcttta ggtggaagat tgtttatcaa cacgtcagtc acagtatttt cgtaaacaat
      841 cggttcacct tctccgcctc cttcttcgtt agtgaagttc atatgcgtta agttcatcac
      901 atcagaagta tttccttgca tatccaagtc aattatcaag gttctttttc cttcattaac
      961 tgctgattca aaccctaaca tttgcgcgca cgtactttta cctacgccac ctttaaaatt
     1021 accgattaca tatttcttcg ctaccacgtt cctacctctt ttcttttagc aatattcatt
     1081 tgatactatt ataacatatt tatttttagt tactaccaaa aaaaagatat atatgtacta
     1141 ccttataatt aataagtaaa aggtagtaca taaataagga aaagctgaaa tatattgatt
     1201 tatagctttc ctcaaagtaa tgacgttatt taatttatcg agaagcagag aatactcgcg
     1261 actttagtca tgtagacgaa tcagattttc gataaaaaag aagccgtcct tcttaagaac
     1321 gacaacaaat cactagatta attttttcaa ctgttcataa aagttttcat gcgttccaca
     1381 taacaatact ggaataacct tgttttctat aatctcgtaa gctactctat atgttgtgcc
     1441 cgcatatttg aacccactag accaaatacc tgataaatcc ccagtttttt gttcaccctt
     1501 atatggatca atcgcaattt catcataaat taaattcaaa aatttttctt tcaatggctt
     1561 ctcttttagc ttttttaaat acttaatgac atgttttgaa ggaacgattt cataagccaa
     1621 tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta tcttcagcaa
     1681 tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca agaatctcat
     1741 ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa tcataaaatt
     1801 ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt gcttcaactt
     1861 cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa ttgccttgtt
     1921 ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt acttagtaaa
     1981 tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta atgcgtcctt
     2041 gctgaataaa tttcaaccac gtgttcatct tcatctacct tataaacaac ccgg
//
LOCUS       DAZUMO010000165         2002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000165 DAZUMO010000000
VERSION     DAZUMO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.173"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACG8ER_002908"
     CDS             <1..377
                     /locus_tag="ACG8ER_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5051415.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            425..1825
                     /locus_tag="ACG8ER_002909"
     CDS             425..1825
                     /locus_tag="ACG8ER_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5051416.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaatgtttc cacagaggac ttctttaatt attgtcgatt agaggaaagt ggtgtaacag
       61 tcactagcgg tacagattta ccgctgttta ttactgatat tcctgaatcg attataagag
      121 cagtattccg tcgttttcct gaaggagaag aagtctggga gaaagaaaca agcttcacga
      181 caaccgagct gctgaaaagt tttacaagca acagcttcaa agtaaatggt ttatcggagt
      241 acggagaaat aaaagtaggg aaaagcgcca gcttagctgt tttcagcgag gacctctgta
      301 ctgatgactg gcaaaaagtg atggctgcgg aagttatcgc tacctatctt gatggtgact
      361 tagtttacaa gaaataatgc ttttcgatat aacgttataa aacgaattaa agagaggtta
      421 aaaaatggaa acaatgaaaa gaaaattcac cttcccacat agttatacat tgatttttat
      481 cttaattatt ttggctgcaa ttttaacttg gataattcct agtggtcagt ttgagacagt
      541 tgttgaaaac gtcgcaggta tggaaaaaac aacggttgtt ccgggaacct atcaccaaat
      601 cgataaggca gattatggac aaggtttttc ctctatacta atggcaccag ctgaagggat
      661 tatttcagct gttgaggttg ttgcttttgt tctcatcgtt ggtggtgcct tcgggattat
      721 cctaaaaacg ggagccatcg atcgagggct gtttacctta gcaaaagttc tgggagacaa
      781 ggggatctta gttattccga tgtcgatgat tctattcagc ttaggaggat caacctttgg
      841 catgagcgag gaagtcattc ccttatttgc ggtgtttatt tctctgatgt tttctcttgg
      901 ctatgattcc atgactgcga tcttgattct atttttaagt acacaagcgg gatatgttgg
      961 gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg tttacggaaa
     1021 tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt caattgtttt
     1081 caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg tttatgagag
     1141 tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt tttcaacaca
     1201 agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt ggggtatcat
     1261 ttccaaggga tggtatatgg ttgaaatttc tgcactattc ttaggattag gtctttttgc
     1321 tgggggcatc gctagatttt ctcaaaagga aattgctgaa gcttttgtcg aaggctgttc
     1381 agatttcgcc tatgctgctg taatcattgg gctagctaga ggagtcttgg taatcttgga
     1441 aggtggaatg attattgaca ctattctaaa cagcttggct aaccttttag gtggtcttcc
     1501 aaaattcctg ttcactacga ttctactcat ttctcaagta attgtttcct tttttgttcc
     1561 atcctcctca ggagctgcgg cattaacgat gccggttatg gcaccattgg gtgatctaat
     1621 gaatgtaagt cgtgatgttg ttgttctaag taaccagttt ggaaatggtt taatgaatat
     1681 tatctcccca actagtggtg tattactggc aggattagcc atcgcagaga ttaatttctc
     1741 taaatggttt aaggttggct tcaaaatatt tgccatctta acccttgttt ctgctgtttt
     1801 gttatacatt gctacactct tttagtctta atcatccatt aataggaggc aatagttatg
     1861 attaaaataa atcaattagc aggaatgaat atccactatt tattttattc tctcaaagaa
     1921 tttttcgctt atcaacagaa aattggtatt tcttctgttg aactttgggg aggtgctccg
     1981 cattttttca tagatgcttt ta
//
LOCUS       DAZUMO010000166         1979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000166 DAZUMO010000000
VERSION     DAZUMO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.174"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8ER_002910"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8ER_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8ER_002911"
     CDS             complement(728..1744)
                     /locus_tag="ACG8ER_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5051417.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          559 a          459 c          308 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cgtaaatcca cgtcactaaa cattgacgta aatttactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZUMO010000167         1952 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000167 DAZUMO010000000
VERSION     DAZUMO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.175"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8ER_002912"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8ER_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5051418.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8ER_002913"
     CDS             852..1040
                     /locus_tag="ACG8ER_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5051419.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8ER_002914"
     CDS             1053..1610
                     /locus_tag="ACG8ER_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5051420.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8ER_002915"
     CDS             1642..1884
                     /locus_tag="ACG8ER_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5051421.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          304 c          404 g          529 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc gg
//
LOCUS       DAZUMO010000168         1819 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000168 DAZUMO010000000
VERSION     DAZUMO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.176"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1783
                     /locus_tag="ACG8ER_002916"
     CDS             2..1783
                     /locus_tag="ACG8ER_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051422.1"
                     /translation="MIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDP
                     DFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRK
                     DYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYS
                     QSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILS
                     GILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYAT
                     FTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYI
                     QTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHY
                     EQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNH
                     QVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIEN
                     PLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRY
                     TGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          589 a          342 c          307 g          581 t
ORIGIN      
        1 cataatcatt atattatcat tgttattttt aacaggaatt gcttatttct tttattttat
       61 tttttctcct gcggacagca atcaaatgaa agaaataaaa gattcgtggg ttttctatac
      121 aaaagatgat cctgatttta agtttgacag caagtacact cgtacgattc ctaccgtgga
      181 taaagacgaa acattcatta tggaaacgac acttgaaaaa ccgttggacg aagcaaattt
      241 actaatcaaa ggaaaccatc aatggattac tgtatatcta ggcgaaaaga ttttatacca
      301 acggaaagat tatcaggaag aaagcaatcc cggactctca ttggctgtga tagatttacc
      361 agcaaattat atcggaaaaa aattgaccct tacagtctca tctccttatc agaactatgc
      421 tggactgcca ccgaaagttt atctaggaac aactggacca ttgattagtt ttatttattc
      481 acaatcagtg ccgcaagtat tgacgatgat aatcgctgtt tttatcgcta tagggacctt
      541 tttctatacg atctacttga tgtataaaca aaaacgtctt gaatactcac ttattctctt
      601 aagttgcttt gctttggcat taggatttga agctgtatca gaagatattt taagtggtat
      661 tctttttgaa ccattcgttc attctttttt atctcattta tttattatct taacgtcagc
      721 tttcatcatc tgctactatt ggagccgaat gttttactat aaaaaatggt acggaatttt
      781 ttgtacgttc caacttttga tttttatcgg cgttctgctt tatgcaactt ttactcctgc
      841 agaacttcct gaattaatgc cgattgctaa tattgctagt gttataagca ctttagtcac
      901 ttcacttgca tgcattggcg aagcatataa gaataaccgt ttttatgtag cttgtacacc
      961 ttggatcgtc ttggtagcca ttatccattg cttgatttac atccaaacgg cattaggtat
     1021 ctatcaaaca acgatcaatt ggtctacgat tttatatatt atcattctaa tcgttattat
     1081 cagctacaat ctaatcgatc acatcttaaa taccgacaaa gatcggagac aagtagattt
     1141 cttaaaaatc aaaaatgatt tgttggagca acattatgag cagcttcgac agcatatcca
     1201 agaagtaaat acacttcgcc tggaatttgt ccaagagatg gagaatctgc aagatttaat
     1261 gcatacggac caaacaaaag ctaaaaacta tgtggaaaag atattagaag aagcaaaaaa
     1321 tttcgaaatg ctgtgttctt tttgcaacca ccaggtgaca aatcttatct tagcgcgtta
     1381 ccagaagtta gctgccaaac ggaatattca gataaccttc caagcagatc ttcccgaaga
     1441 acttcctatc aacgatgacg atctggctca acttttgatc cacgctttag agcattcctt
     1501 tagagaaact catgccatcg aaaatccact gtatcgaaaa atttatttgt cgatacacga
     1561 acaagaggaa catttcgtca tttgttgcga acatagtgca cattacgata caaacctatt
     1621 tagtcgaggc atcacagaag aattagatga tcaggaacgt tttgacttaa tgatgatgaa
     1681 agacgtagcg gatcgttaca caggaacact aacgcaggaa aaagatactt tcattgatcg
     1741 gatcactgtt caactctctc gaaattaccc gaatcctgta taaaaaatag cggaatccat
     1801 atatgtggat tccgctatt
//
LOCUS       DAZUMO010000169         1749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000169 DAZUMO010000000
VERSION     DAZUMO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.177"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      188..354
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            528..1634
                     /locus_tag="ACG8ER_002917"
     CDS             528..1634
                     /locus_tag="ACG8ER_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5051423.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          513 a          284 c          360 g          592 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg atcggtcttt
      541 tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat acaaaaagtc
      601 tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc ttagctcgtt
      661 cagtcaaact acactattta cttcacccta aaatagcagg atacggttta gccttatcga
      721 ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac ggcgcacaac
      781 gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat ctttttttga
      841 ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat ctaaaaaaac
      901 cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag attgctggag
      961 cattgatgat cctttcgatt gcgtgggtca tattttgggc agcggcggtt ccatttaaaa
     1021 aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca ggcggggtat
     1081 tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat gaaaggatcg
     1141 cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg acccattcgt
     1201 tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt atcacaaaaa
     1261 aagggtatct tccagagaca gaaacagatt ttattttttc gattattaca gaagagcttg
     1321 ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg agaatctttt
     1381 gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga ttcggaactt
     1441 tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg ccaatgactg
     1501 gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt tctttaggaa
     1561 tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttggcg ctctatcgac
     1621 ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata aagtacaatc
     1681 ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa agtatctcaa
     1741 aaaatcata
//
LOCUS       DAZUMO010000170         1705 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000170 DAZUMO010000000
VERSION     DAZUMO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.178"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1509)
                     /locus_tag="ACG8ER_002918"
     CDS             complement(28..1509)
                     /locus_tag="ACG8ER_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051424.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          525 a          232 c          298 g          650 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
     1561 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
     1621 gttatttaat ttttttgtta ccttgtttat tctattgttt atttcctagg gtattttgtc
     1681 aagatattct tgtttttaag gcgga
//
LOCUS       DAZUMO010000171         1622 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000171 DAZUMO010000000
VERSION     DAZUMO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.179"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..277
                     /locus_tag="ACG8ER_002919"
     CDS             17..277
                     /locus_tag="ACG8ER_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051425.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(639..878)
                     /locus_tag="ACG8ER_002920"
     CDS             complement(639..878)
                     /locus_tag="ACG8ER_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051426.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1164..1478
                     /locus_tag="ACG8ER_002921"
     CDS             1164..1478
                     /locus_tag="ACG8ER_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051427.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          558 a          219 c          286 g          559 t
ORIGIN      
        1 taaggatgga tgaaatatga ataacgaaac tatcatttta ggaaaaaaat atatttgcca
       61 acctattggc ttgaaacaaa aagtgattgg agaagtaatc agtaaattag aaaattgtgt
      121 tgtgatctgc gtagacgaat atcatgacgt cgatcgcaat gaaatattag aaaaatgcgg
      181 aaaagtagtg gcaaaatata ctcacgttta tgagttggaa acaacagaat atctatataa
      241 ctatagcaac aaatatgaac atactttagt ttcataaaga tttaaataaa gtgccttcaa
      301 aagcgagaag accaagcttt tgaaggcact ttatttagtt ggcagtgatt gttcatgatt
      361 tttgatttat tcaaaaggtt cgcctagaga atctgtctct gtaacgaggc ctggtggaga
      421 aactcgataa aaacctatac tgccagaact gtcctatttt cggatagaca aactagtagt
      481 tttaaaaaga tcatccgatc caatcatttc aaaaaatgca aaactgatat caggatcatt
      541 tagctcaggc ttgttatcgt aaagcatttg taaaggttgc atttagttag gagtaaacgc
      601 gaaactatca tcattatttg ctgaagaaga tgaagaagct aattcagagt ttgtttgaaa
      661 taaaggagac gtgctgacag tttctttacc ctgttttttg cagaggtaga aacttttata
      721 tcggaagttt ctaccggaga atttttctca tttgtagaaa ttgttgaatc gatactattc
      781 gaaatttgcg tatctgaagg tatgttcttt gatgcagaag tcccacaagc agtaagtaac
      841 aataaatttg ataaactgat aaacagaatt cttttcatta tgtacacact ccttatagta
      901 tcgtttagca tacacctatg ctcagtatta ggcaaaatca atgcaaaact atcaagtctt
      961 ttatagtatc tattagtctt agtaatacat atatggagag ggtgatatcc cagttcaaac
     1021 cagatacatt ggttaccatc tgtattaacg attatgcctc taaacgcttt ttttatgaaa
     1081 aaatttataa gtgaaaacgt aggtatgata ttatctagag gtaatttttt tgggtattta
     1141 aatagttgct aggaggatag cttttggatt tttacagtaa ttttatctta ataatagcaa
     1201 tattattact tctaaatatt tggttttttg ataagagtag aaatgcaggg ataggtttta
     1261 gaaccaaacg aagtacgtct tctgaaaaaa agtgggtata ttcacagact attttttacg
     1321 gaggggttat ttcaatcagt ttactttctt caacacttta ttcttttaat gttatagacg
     1381 tttccatgtc aaattttata tctattattg gaatccttat ttctgcaatt attacacaac
     1441 tacttcttgt gtttgaagag aagtcaaaaa ataattaaag caattatgcc ttttagtaca
     1501 ggaattatta cttcttacaa atgtatagcg aatgtgaagc ctgctgtttt aattgttcta
     1561 gaaaaacttt tgagtgaaca attattttat gttaggatga attaatttct ctagagttta
     1621 ta
//
LOCUS       DAZUMO010000172         1536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000172 DAZUMO010000000
VERSION     DAZUMO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.180"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8ER_002922"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8ER_002922"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZUMO010000173         1499 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000173 DAZUMO010000000
VERSION     DAZUMO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.183"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            759..1145
                     /locus_tag="ACG8ER_002923"
     CDS             759..1145
                     /locus_tag="ACG8ER_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5051428.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1239..1430
                     /locus_tag="ACG8ER_002924"
     CDS             1239..1430
                     /locus_tag="ACG8ER_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051429.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          557 a          232 c          209 g          501 t
ORIGIN      
        1 accacgatag gtaactttaa acaaacatga agagacttta ataagacttc tttatctgaa
       61 aacacttttg tttctctctt tcctttctac atcattaaat agtattttat acgttatcta
      121 aaatactatt cctctgtcaa aataagctaa cttacttcat tataaaatat ccacttatcg
      181 ctttcatgta tattccaccg taaaaagtac attttatcac cgagaactca tcatttttca
      241 tcatgatgaa tactatttac atatatctag ttcaaagaca ataattatta tgatccgtta
      301 ttatctaagc actatacaga taataggaat ttttcgtttc taaatattcc aatttgggaa
      361 tccaataaaa attttcgttc aaatacgtgt ctattcgaac tcttttctag ttgttaatta
      421 tgaaggggga agaaataaaa aatacaatct atacacgaat aagaatatta tccaaaagga
      481 ggttttagaa aagatttatt atacgtaaaa agctcaagac attttcttag ccataaggac
      541 atgtttgata tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca
      601 ttatcaacta aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag
      661 cttatagaag ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc
      721 tcttttccct ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg
      781 taaatgccca gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt
      841 ataaaaaaca agaagaaatt caaaaacatg aacaacttga tcaactccca ccatatttgt
      901 ttactaaaga tactataaaa tttaaacaac cgattaatgt tttaaatgtg actctccttg
      961 ttgaagatat aaaattagca aaaatccttc ctttgttgaa agaacctgaa caaatttttc
     1021 ttatcgaaaa atttatcttt gataaaactg ataaagaaat tggaaaggtt ctggggatta
     1081 caagacaggg tgcgacaaat ttaaaacatc gactttacaa aaaaattcaa aaaatgctgg
     1141 tttaatatct gcataataac taattttgta tgcttctttg ctcgttctcc tccttcccaa
     1201 agaaatagat tataagtcca tgggaagaag gtagatttat gaattcttta aaagaagatt
     1261 tcattttagc aaaggctgga aatgaagaag ctgttgaggc aattctcaaa agatttagct
     1321 ctttgattca taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat
     1381 gtatgcttgc aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag
     1441 ggagtctgtg acataagtaa aaagatagtt tcaaatttcg aacaataaat ttgaaacta
//
LOCUS       DAZUMO010000174         1466 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000174 DAZUMO010000000
VERSION     DAZUMO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.184"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(191..490)
                     /locus_tag="ACG8ER_002925"
     CDS             complement(191..490)
                     /locus_tag="ACG8ER_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5051430.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            841..>1466
                     /locus_tag="ACG8ER_002926"
     CDS             841..>1466
                     /locus_tag="ACG8ER_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5051431.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKV"
BASE COUNT          496 a          231 c          205 g          534 t
ORIGIN      
        1 acacttttaa ttaacattat tagtttatac tagctgtttt tgtgttaatt aaaggataaa
       61 atacaatata aagaaacatc tgtttcttta tttagaataa aaaaatacag actctgttca
      121 aataaatgaa cctagcctgc attatgagat tgactatact ttttaattca ttgagatgaa
      181 taactcgttt ttataaatgc ccaaaagtaa tcggtgccga agccattcct cgacctaatt
      241 ctttttcaaa ttttcctatt gttgatatct ccatataaaa cggtgatact ggtttagaca
      301 attctcccct gattgctaac ggacgtaaac tacgttccaa attatttatg actttaggat
      361 aatactgacc tttttctaag cctgtctttg cgtctatcaa aagtatacgt tccttttctt
      421 tgatctctgg atttaaaacg agatcatata attttagtac catgttttct atttcttcac
      481 ttttagacat cacgatttcc tcccaaaaat atatttcttc ccactacatt ctataaacaa
      541 agtatacgct acatctagat ttttttaaat cattactgga ttgttaaaac ctttcaatac
      601 actgttatgg caacgatttt tattttaaaa acattctaaa ttaaatcagt aaaaattttt
      661 attctaagat ttttattaga attacgccta atttgtgtct ctcagggcaa ttctaagaac
      721 gtttcaagag atagcgacac tatcgttatc gaaaaaggcg aaaacctatc tcattttctg
      781 ctaatgtaag ttggtttagc ttttgttaat atgactttat gaaaatggag gtaattaaat
      841 atgatttttc ctaaagttaa tgaagcaagc ttgaataaag gatccttttt tcatatctat
      901 gaaaacgaag attttgaact gtataatatc ctttcacaaa aagaggtcat cgaaaaccaa
      961 ataatctgcc atccaattat ttacaaatct attttcaagt atgcattttt tgcttccaat
     1021 gattttcaaa gaatggagat taccgactgt atattcgaaa actgtgattt ttcaggctgt
     1081 atattgacaa aaagcatgtt gcatcgcgtg aaatttgtca attgtaaatt aacaggtact
     1141 aaattgatgg aaagttatat tgggaacacg ttattcgaga attgtaaaat ggactatgta
     1201 aatttatctg gttcaaacat aaaagaaagt acctttgagc actctgtctt atcaagcgca
     1261 gattttgtag actgttccct cactaaaata atgttttcta ctaatgattt agaaaatgct
     1321 agcttttttg atactgattt aaaaaatatc gatttaagta gcaactcctt tgaaatgatt
     1381 gaagtacgct cggaacatct aaaaaattgt acgcttaatc agttgcaagc acttggtttt
     1441 gctagaaaat ttttacaaat aaaagt
//
LOCUS       DAZUMO010000175         1459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000175 DAZUMO010000000
VERSION     DAZUMO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.185"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACG8ER_002927"
     CDS             complement(15..1154)
                     /locus_tag="ACG8ER_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS5051432.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          382 a          316 c          263 g          498 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctg
//
LOCUS       DAZUMO010000176         1457 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000176 DAZUMO010000000
VERSION     DAZUMO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.186"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..787
                     /locus_tag="ACG8ER_002928"
     CDS             26..787
                     /locus_tag="ACG8ER_002928"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5051433.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(902..1342)
                     /gene="fabZ"
                     /locus_tag="ACG8ER_002929"
     CDS             complement(902..1342)
                     /gene="fabZ"
                     /locus_tag="ACG8ER_002929"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5051434.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          489 a          276 c          267 g          425 t
ORIGIN      
        1 aatagtaaat tggaaaggaa cagttatgaa gaaaaataaa gttattttaa gtattagttt
       61 agcagcagca ttgctgtttg ctgctggttg ttctgcaaac aacacgactt cttctagttc
      121 gtctacagaa agttcaaaaa gtacatctac tgcggaaagc acgactgcca gtagcgtcga
      181 tttgaacgca ttggaattac cacaattgac gaaagatgtc gccgaaaatg aagatctggt
      241 cgaaatggtg acgaataaag gcacaatcaa aatcaaatta tttcccgaac acgcaccaaa
      301 agcggttgaa aactttatga cacatgcaaa agacggttac tacaacggac tgactttcca
      361 tcgcgtcatc aaagatttca tgatccaagg tggcgatcct aaaggcgatg gcactggagg
      421 ggaaagcatt tggggagaag gatttgaaac agagatcaac aaccaccttt ataatataag
      481 aggtgcgtta tctatggctc gctcacaaga tcctaacagt aacggcagcc agttctttat
      541 cgttcaaaat acagatgata tgcatgatgg tcttttaaaa gacgactatc ctcaagcaat
      601 catcgacgct tataaaaacg gcggttatcc ttcactagat ggccattata ctgtcttcgg
      661 ccaagtgatt gaaggaatgg atgtcgtgga tgctattgca aatatcgaaa cagatagttc
      721 agacaaacca aaagaagaag taaaaatcga taaaatcaat atactgcaag aagcaaaaca
      781 aaaataaaaa aataacgtct gaatcctgca tagatgacaa cgtatgctag gattcaggcg
      841 tcttcttttt acataaaaag caagtaagct tccttttgtt ttgctatatc aggttttata
      901 gtcaagcatt ttggttgacg atgaacgtga actcgcactc acagactttt ttatcatcta
      961 catatgctgc agataacgct gtaccgactg atcccttcat tttggtgatt tcaaaatgac
     1021 atttcagcac atctcctgga acaacttttt gacggaattt ggccttattg atcccaccga
     1081 tataagctct ttttcctaga aactcttctg attttaggat aagtatcgaa cctgcttgtg
     1141 ccagtgtttc tagaatcaac gcacccggca tcactggatt gtcaggaaag tgaccttgga
     1201 aaaaatcttc attgattgtt acattttttg tcgcaacgat cttttttcct ggttctaaag
     1261 tatctacata atcaatataa caaataggat aacgattagg aatcaattcc attacttcag
     1321 tagcagataa tacgtaagac ataattaaac tccttctttc aggcaattgc cattttcatt
     1381 actttgacta tcaaagtatc atattcctaa atatttagca atcttttttt aatttaagaa
     1441 aaaatagttt gacaatc
//
LOCUS       DAZUMO010000177         1444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000177 DAZUMO010000000
VERSION     DAZUMO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.187"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..286)
                     /locus_tag="ACG8ER_002930"
     CDS             complement(<1..286)
                     /locus_tag="ACG8ER_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5051435.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDN"
     gene            complement(283..936)
                     /locus_tag="ACG8ER_002931"
     CDS             complement(283..936)
                     /locus_tag="ACG8ER_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5051436.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1224..>1444
                     /locus_tag="ACG8ER_002932"
     CDS             1224..>1444
                     /locus_tag="ACG8ER_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5051437.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEK"
BASE COUNT          463 a          247 c          160 g          574 t
ORIGIN      
        1 tattatcttt gatttgtatt tcattcttat tatttttttc ttttatgaat acttttattt
       61 cactactctt ggaataaaac tccaaagttt gttctatata ttttatttct tttccattca
      121 tattactaga aatttcattt gccatattat gaatcttttc ttttctcgtc tccaaatatg
      181 ttttaggaaa gataaaaaaa attaatgaat gaactaatat aattattatt ccaagaacag
      241 aaaatatttg tatgaacatt tttggaaata tctttaattt tttcattttc tctccaattt
      301 atatcctaca tttcttatag tagttataca gtctaattgt aatttttttc taagttcttt
      361 tatatataca tctattactc gatcataagg agtttcttcg ctatctttcc atacataatc
      421 aataatttgc attcttgtta acacttgtcc attattatcc aataaacatt ttaatacttc
      481 tagttctttt gcatttacat ctatttcttc accatttatt ttagctgtat agctattaaa
      541 attaaccgat agatccttat attcaaactt ctctaaatgt ccataattct tcttaattaa
      601 agaatctatt ctagctttta agaccggcaa tgaaaatggc ttttctacat atccgtctac
      661 taaattagta aatgcatcaa ttttatattc ttcatcacta aatgcagtca agattagaat
      721 tggtaaattg cttttctttc taatttcttt caacacttct aaaccattta taaaaggtat
      781 ctgaatgtct aagataacca aatttatatc gctattaaat tttgacaagg cttctcttcc
      841 atctttagct tgaataacag tatatccaaa ttctgaaaga tattcactta ttccctctct
      901 tatcatatta tcatcttcaa cagttaatat tttcatatat acctccactt aagcctatac
      961 actagatatt atacagcata ttatggctct ataatatcta gtgttggtga caccttctgt
     1021 tgccaatact ttttcttttt tctaataaat gcaaacagac accttgaaat cctttagaat
     1081 aaagggttct ataatatttt gtgttgttgg aaaatcgaag gcgattttct actaacactt
     1141 tttcttcgtt tcaactcaaa aagagactca cttttggtaa aatttagtcg accaaaacat
     1201 caccctacca aaggagtcgc tctatggata atcatactag aaaattactc ggattaacag
     1261 ataaacattt attttttgat gaagaatggc tgattgaaaa agaatataaa ggcgttcaag
     1321 cgcagtttat taaagggaga ttggatgact caccttccca ttgtgaacac tgtggatcga
     1381 ttcgtattat ccgcaacggt tcctatacga cacgtactca gatattgaaa gtcaaagaaa
     1441 agtt
//
LOCUS       DAZUMO010000178         1425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000178 DAZUMO010000000
VERSION     DAZUMO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.188"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1425
                     /locus_tag="ACG8ER_002933"
                     /pseudo
     CDS             5..1425
                     /locus_tag="ACG8ER_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          590 a          223 c          319 g          293 t
ORIGIN      
        1 ggtcatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccagag
      121 aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agagcgaatc gaaaataaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 agtagaaaaa cttgaaagac aagaagagat aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgaga atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cggaaaaagg aaaagaaaac cttcttcctg aagtacaagc
      481 aatcattgac agcggacgag tgacgcaatg gaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgta
      661 ccaactgatt ttttgaagga gaaagaaaac gatcattttt atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcatactca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaatagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaa
//
LOCUS       DAZUMO010000179         1424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000179 DAZUMO010000000
VERSION     DAZUMO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.189"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8ER_002934"
     CDS             complement(150..1337)
                     /locus_tag="ACG8ER_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS5051438.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZUMO010000180         1420 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000180 DAZUMO010000000
VERSION     DAZUMO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.190"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG8ER_002935"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG8ER_002935"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5051439.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          495 a          184 c          236 g          505 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatactata
//
LOCUS       DAZUMO010000181         1375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000181 DAZUMO010000000
VERSION     DAZUMO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.191"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..109)
                     /locus_tag="ACG8ER_002936"
     tRNA            complement(25..109)
                     /locus_tag="ACG8ER_002936"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Leu,seq:caa)
     gene            complement(127..198)
                     /locus_tag="ACG8ER_002937"
     tRNA            complement(127..198)
                     /locus_tag="ACG8ER_002937"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Gln,seq:ttg)
     gene            complement(208..280)
                     /locus_tag="ACG8ER_002938"
     tRNA            complement(208..280)
                     /locus_tag="ACG8ER_002938"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:His,seq:gtg)
     gene            complement(342..426)
                     /locus_tag="ACG8ER_002939"
     tRNA            complement(342..426)
                     /locus_tag="ACG8ER_002939"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Leu,seq:caa)
     gene            complement(447..517)
                     /locus_tag="ACG8ER_002940"
     tRNA            complement(447..517)
                     /locus_tag="ACG8ER_002940"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Cys,seq:gca)
     gene            complement(563..634)
                     /locus_tag="ACG8ER_002941"
     tRNA            complement(563..634)
                     /locus_tag="ACG8ER_002941"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(600..602),aa:Gln,seq:ttg)
     gene            complement(646..718)
                     /locus_tag="ACG8ER_002942"
     tRNA            complement(646..718)
                     /locus_tag="ACG8ER_002942"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:His,seq:gtg)
     gene            complement(721..794)
                     /locus_tag="ACG8ER_002943"
     tRNA            complement(721..794)
                     /locus_tag="ACG8ER_002943"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Trp,seq:cca)
     gene            complement(801..881)
                     /locus_tag="ACG8ER_002944"
     tRNA            complement(801..881)
                     /locus_tag="ACG8ER_002944"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Tyr,seq:gta)
     gene            complement(883..958)
                     /locus_tag="ACG8ER_002945"
     tRNA            complement(883..958)
                     /locus_tag="ACG8ER_002945"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(923..925),aa:Phe,seq:gaa)
     gene            complement(994..1066)
                     /locus_tag="ACG8ER_002946"
     tRNA            complement(994..1066)
                     /locus_tag="ACG8ER_002946"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Asp,seq:gtc)
     gene            complement(1076..1148)
                     /locus_tag="ACG8ER_002947"
     tRNA            complement(1076..1148)
                     /locus_tag="ACG8ER_002947"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1113..1115),aa:Val,seq:tac)
     gene            complement(1164..1235)
                     /locus_tag="ACG8ER_002948"
     tRNA            complement(1164..1235)
                     /locus_tag="ACG8ER_002948"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1200..1202),aa:Glu,seq:ttc)
     gene            complement(1250..1338)
                     /locus_tag="ACG8ER_002949"
     tRNA            complement(1250..1338)
                     /locus_tag="ACG8ER_002949"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1302..1304),aa:Ser,seq:gga)
BASE COUNT          387 a          362 c          318 g          308 t
ORIGIN      
        1 agaaagttgc tatattgata attataccgg cggccggggt cgaaccggca cgtcctcaac
       61 ggacactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca aatttaaaaa
      121 taataactgg ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta
      181 ccgcttggct ataccccaag aataaagggc gaatgatggg aatcgaaccc acgaatgccg
      241 gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catcataaaa gatgttattt
      301 aatttctcaa taaaaaacta agatgaagac atcttagtca ataccggcgg ccggggtcga
      361 accggcacgc cctcaacggg cactggattt tgagtccagc gcgtctgcca attccgccac
      421 gccggcaagt tgatttatta gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt
      481 tttgcagacc tctgccttac cacttggcta taccgccatt atactattat caatctacaa
      541 aataaagaat aaattataga aactggggta gctggattcg aaccaacgca tgagggagtc
      601 aaagtccctt gccttaccgc ttggctatac cccaagaata aaaagggcga atgatgggaa
      661 tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca
      721 tggcaggggc agcaggaatt gaacccacac taacggtttt ggagaccgct gttctacctt
      781 taaactatgc ccctataaaa tggaggagag tggattcgaa ccactgaacc ctaaggaacg
      841 gatttacagt ccgtcgcgtt tagccacttc gctactcctc catggtggcg cgggacagaa
      901 tcgaactgcc gacacacgga gcttcaatcc gttgctctac caactgagct accgcgccat
      961 attttaaaat tattaattga ataacaagaa cgacggttcc gacgggaatc gaacccgcga
     1021 tctcctgcgt gacaggcagg catgttaacc cctacaccac ggaaccagtt cattatggag
     1081 gttaacggga tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc cagctgagct
     1141 aaacctccaa cattcaatta aaatgacccg tacgggattc gaacccgtgt taccgccgtg
     1201 aaagggcggt gtcttaaccg cttgaccaac gggccaggta aatttgctac ggagagtaag
     1261 ggattcgaac ccttgagaca gtttgcacca tctacatgat ttccaatcat gctccttcgg
     1321 cctctcggac aactctccag agtgaaagaa gctcaatact ctttctcaaa atgag
//
LOCUS       DAZUMO010000182         1373 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000182 DAZUMO010000000
VERSION     DAZUMO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.192"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..>1373
                     /locus_tag="ACG8ER_002950"
     CDS             105..>1373
                     /locus_tag="ACG8ER_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5051440.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLN"
BASE COUNT          446 a          278 c          256 g          393 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aat
//
LOCUS       DAZUMO010000183         1368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000183 DAZUMO010000000
VERSION     DAZUMO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.193"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1368
                     /locus_tag="ACG8ER_002951"
     CDS             82..>1368
                     /locus_tag="ACG8ER_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFS5051441.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIK"
BASE COUNT          466 a          256 c          259 g          387 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaaa
//
LOCUS       DAZUMO010000184         1364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000184 DAZUMO010000000
VERSION     DAZUMO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.194"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8ER_002952"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8ER_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS5051442.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZUMO010000185         1346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000185 DAZUMO010000000
VERSION     DAZUMO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.195"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..865)
                     /locus_tag="ACG8ER_002953"
     CDS             complement(251..865)
                     /locus_tag="ACG8ER_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5051443.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          447 a          263 c          221 g          415 t
ORIGIN      
        1 ttccactact ttttattttt ctgacactac atattcttat aactcatttc tcataactgt
       61 gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc
      121 atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt
      181 ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac
      241 atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa
      301 cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat
      361 ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa
      421 attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc
      481 cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg
      541 ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct
      601 gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt
      661 caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac
      721 ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc
      781 aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat
      841 aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata
      901 gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg
      961 tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca
     1021 tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc
     1081 ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt
     1141 cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga
     1201 gccttttttt attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg
     1261 caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa
     1321 aaggaatggc ttatcccgat acaaca
//
LOCUS       DAZUMO010000186         1302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000186 DAZUMO010000000
VERSION     DAZUMO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.196"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>1302
                     /locus_tag="ACG8ER_002954"
     CDS             52..>1302
                     /locus_tag="ACG8ER_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5051444.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAZUMO010000187         1288 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000187 DAZUMO010000000
VERSION     DAZUMO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.197"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..>1288
                     /locus_tag="ACG8ER_002955"
     CDS             2..>1288
                     /locus_tag="ACG8ER_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS5051445.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          433 a          250 c          246 g          359 t
ORIGIN      
        1 tatgaatgat tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga
       61 ggtctcttac gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc
      121 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
      181 tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      241 tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac
      301 ggctcaaagt tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat
      361 tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc
      421 tttgaccacc gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa
      481 gaagatcctc cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga
      541 taagatgagc tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac
      601 gcgtaaatta cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga
      661 attcttggtg acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa
      721 tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga
      781 gttgcgtatc cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa
      841 actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata
      901 caaaacatgg aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat
      961 tgatcgattg cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt
     1021 agttgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc
     1081 cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg
     1141 catcaccaac gcaatgatct atccttattc caatggaaaa atagaagcga agaataccca
     1201 cataaagaca atgaaacgag tatcctacgg atttaaatca tttgagaaca tgagaattag
     1261 aatctttttg atcaatcaat taatcaat
//
LOCUS       DAZUMO010000188         1284 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000188 DAZUMO010000000
VERSION     DAZUMO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.198"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1170)
                     /locus_tag="ACG8ER_002956"
     CDS             complement(76..1170)
                     /locus_tag="ACG8ER_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS5051446.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          381 a          272 c          197 g          434 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caat
//
LOCUS       DAZUMO010000189         1258 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000189 DAZUMO010000000
VERSION     DAZUMO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.199"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1167
                     /gene="xerS"
                     /locus_tag="ACG8ER_002957"
     CDS             82..1167
                     /gene="xerS"
                     /locus_tag="ACG8ER_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS5051447.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          503 a          212 c          191 g          352 t
ORIGIN      
        1 caattgacaa aacactctat aattggtata gtccaattat agagatactc ctctccccta
       61 cttatcgaaa ggatgaataa catgaaaaat acagaaatat atcaaaaaat agatacaatc
      121 atacttcata tgcccgatta tataaaagat tttgttcaag atcgtgagga taaagatcaa
      181 tcacctcgaa ctactttaga atatctaaaa aattataagc tcttttatga atggcttctg
      241 agtgagtcta tcgttcccga tacttcaatc acctcgataa gacagttaac tgcctatgat
      301 ctaaacttgt acaaatctca tttgaaacgt cgcacaaagg aaaatgtaaa aaaagagaca
      361 acaaaaacta agctcaaaga taataataac ttaggtctat ctacttctac tatcaataga
      421 aatatcacag cattaaaggt tctatttaaa tatctttcga aaagttccaa taatccttta
      481 ggaaaaccat acttagaaaa taatcctatg gatcaagtag caactatcac agataaaact
      541 acgttagctg cccgtgcaaa cgcaatcgag aaaaaattat ttctagacga agatacacaa
      601 aattatctag attatattgc caatgaatat aaaaaaactt tgagcaaacg agctttgatc
      661 tattatcaac gagatgtaga aagagacctg gcgatcaatg cattgatttt aggatctgga
      721 ttaagactct ccgaggttgt aaatattaat ttagaggacc tatctttaga aaagaataat
      781 gtggtagtaa ctagaaaagg aaataagaga gacgcagtaa atatagcagc atttgcaatg
      841 gaatatttag ctaattatct agctattcgg aaagaacgat acaaagtaac agaaaacgaa
      901 aaagctttat ttctagcaat ttatcaagga gaagccaagc gtatatccgg tattgcaatc
      961 gaacggatgg tagcaaaata ttcaaaagga ttcagagtcc aagtcagtcc acataaatta
     1021 cgtcatactt tggctactag actctatcaa cagacaaatt cattggtatt aactgcccaa
     1081 caattgggac atacaagtac aaatacaaca actttatata ctcatataga caatgcagct
     1141 acaattgatg ctttgaacgc tttgtaaatg tagtaaaaaa tagtataata atgaagcctg
     1201 tctctaaaat tagataaaag cccaacttta aaggcaagct tcattatcat ttttatat
//
LOCUS       DAZUMO010000190         1246 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000190 DAZUMO010000000
VERSION     DAZUMO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.200"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8ER_002958"
     CDS             200..742
                     /locus_tag="ACG8ER_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051448.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG8ER_002959"
     CDS             898..>1246
                     /locus_tag="ACG8ER_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS5051449.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZUMO010000191         1222 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000191 DAZUMO010000000
VERSION     DAZUMO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.201"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..681)
                     /locus_tag="ACG8ER_002960"
     CDS             complement(325..681)
                     /locus_tag="ACG8ER_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5051450.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(695..1105)
                     /locus_tag="ACG8ER_002961"
     CDS             complement(695..1105)
                     /locus_tag="ACG8ER_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5051451.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          392 a          226 c          171 g          433 t
ORIGIN      
        1 caagtggttc ttttttaaca actcagttat cttatcatgt catttctaac atgtcaacaa
       61 ctattttata tgttttttaa aatactattt tattgctttg ctacttaaaa caacagaaat
      121 gaatatatca tgtaaattta cacatgtcaa tatattttta actctatatc cccacctaga
      181 tttgaatttt gaccacataa attgagcatg tctctatagt agaaaaataa agtcaaaaaa
      241 agaacacata acacgcttga tgctaagtgt tcgactgtta caactgtatg aaatgctcag
      301 tgacttgatt aaaacagtta aagtctattt atttaagata agctgttcca tcaactgctc
      361 attgtatcca ataaatagaa cttcatcgat aataatcaaa ggacgtctga ggagattggt
      421 atgttgtgtg cataaaaaaa tcagttgttt ttttcctaca caggaaaggt caatcgcaat
      481 gttgtgaaaa gcctcagacc aagtcgcaaa tagctgttct gcttgcactt cttcaaaact
      541 gcataattct tctgcttctt ctgtacttag atcgcgttct tctaagtcaa caaattgata
      601 aggcaattga tggtgttcta accattgttt cgccttcaga cttgttacac agtgcgctga
      661 tccatagact ttcacttcca cgaaggctcc ctccctatga atttaagatg ttattgtttt
      721 gtaaatagat cagatttctg gcaacacatc gaatttcacg agggacaaat gaccggatct
      781 catcttcatt aaatccgatt agtattcttt tttcatcaat caaaataggt cgtctcaata
      841 atgaaggata ttctttgaat aagacgatca tttgctttaa agttaattcg ttaaaatcta
      901 tgcccagctt ttcgtaaatt ttcgaccgag tggaaataat atcttgaact cctccttcag
      961 tcgctgccaa gatctttttg atctcacttt ctgttaacgg ttcggcaaaa atattcttct
     1021 cttcaaattc aagttgattc gcttgcaacc atgctcttgc ttttctacag gaagtacagc
     1081 taggcgaagt atatactttt atcattttct cactcctttt taattataat ccaattctac
     1141 agtaatttct tttaaaatac caattattag ctaataaaat ataaaaaaaa tcaaaataac
     1201 tgattattta cgtttgaata at
//
LOCUS       DAZUMO010000192         1146 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000192 DAZUMO010000000
VERSION     DAZUMO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.202"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..330
                     /locus_tag="ACG8ER_002962"
     CDS             64..330
                     /locus_tag="ACG8ER_002962"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS5051452.1"
                     /translation="MTSTYASFDKDNLYYDFPSVSGFSSMNNKKYLLMLTRLGYSTSN
                     VRAKRNGGTVITDILLSNQYQVIPNQEIQHQATSGVKIIESEVS"
     gene            433..708
                     /locus_tag="ACG8ER_002963"
     CDS             433..708
                     /locus_tag="ACG8ER_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051453.1"
                     /translation="MTNLESRQFDPCWHQIDAQTKELLLGWEENQVVRDFSLNFIMID
                     YYNLATSMQRFPNYGMDIDSFKQKSFSGEIQNEESNNLVIPILYEPG"
     gene            718..924
                     /locus_tag="ACG8ER_002964"
     CDS             718..924
                     /locus_tag="ACG8ER_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051454.1"
                     /translation="MDGEEYPYKVAFDDLIQLSNLPKDSNMKIAGEFFAPGFLVGLVI
                     SSIATGCFLFLLFLDYIKKTEDIP"
BASE COUNT          394 a          184 c          204 g          364 t
ORIGIN      
        1 acaatcaacc aaactctaac agaatttgaa gtatatcaag aatattggga accactaaaa
       61 aaagtgactt caacttatgc atcctttgat aaagacaatc tctactatga tttcccctca
      121 gtatcaggat ttagttcaat gaacaataaa aaatatttac taatgttaac gagattaggg
      181 tactcaacaa gtaacgtccg tgctaaacgg aatggtggta cagttattac ggatattttg
      241 ctaagcaacc aataccaagt aattcctaat caagaaatcc agcatcaagc gactagcggt
      301 gtaaagatta tagaaagcga agtgagttaa acctcagata ttgcttcttt ttcatttgac
      361 gttctagaag taacaaaaga tgagttactt tatgtttatt ttcccgtcaa cttggccaat
      421 aatttgcact atgtgaccaa tttggaaagt cgacaatttg atccctgctg gcatcaaatt
      481 gatgctcaaa cgaaagaatt gttgctaggt tgggaagaaa atcaagtagt tcgtgatttc
      541 tcattaaatt ttattatgat tgattactat aatttggcga catcaatgca acggttcccg
      601 aattatggta tggacataga ttcattcaaa cagaaaagct tttctggaga aattcaaaat
      661 gaggaaagca ataatcttgt tatacctatt ctctatgaac caggttaggt ttttactgtg
      721 gacggggaag agtatcctta caaagtagct tttgacgatt tgattcaatt gtctaactta
      781 ccaaaagata gtaatatgaa aattgctgga gaatttttcg caccaggatt cttggtaggc
      841 ttggtaatct ctagtattgc aactgggtgc tttttatttt tactttttct ggattatatc
      901 aagaaaacag aggatattcc ttaggaaaat cacaatctaa atcttcccta gaaactatag
      961 aatgacaagg gggatataag caaattccta aatgattaaa tagggtgcgt tactagttag
     1021 ctccatagta agattttttc aaaattttct tgaggttgga tcaaaaaggg ctcttttcaa
     1081 ttagattaaa gacactcata caaaatagag aaacgaactt ataatcgcgt tcgtttccct
     1141 atttaa
//
LOCUS       DAZUMO010000193         1105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000193 DAZUMO010000000
VERSION     DAZUMO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.203"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..>1105)
                     /locus_tag="ACG8ER_002965"
     CDS             complement(80..>1105)
                     /locus_tag="ACG8ER_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS5051455.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          361 a          181 c          166 g          397 t
ORIGIN      
        1 taaatggaaa gttttgtctc gcttaatttg acaaataata tttttagaaa aagcagtcgg
       61 actttctaaa agaagttcac tattcgatcc tgtcgatgat ttcttcagga aaatcgtcta
      121 tttgcttgtc aattttgctg tctttgtatt tctcttcttg gatagtcaac aaaatctttg
      181 taagattccc ccagtctact gctcttggcg gatcgagcca aaccactact tgagatgcac
      241 tttcttcata tagattattc ctattcgtga ttataacatc cgggaactca ggctttcctg
      301 cctctatcac ttccgtgttc agattaatgt acaagcttaa atttttcttg ataaattgat
      361 tataggcagc accataagaa aaatcgatga tgatctttac tggttcagaa atcagttgaa
      421 tgggtatgtg attgatcaat aataataaat aactgaaaaa aagaaaaaat ttatcttgaa
      481 gcaactcttt gcgttttgac gttagatcat ttatatactc tatcaaatag caataaaatg
      541 ctggatagtt ttctctaaaa aaagaaacat caagtggaaa ttcatcagct ttattaatag
      601 tgtaatgcaa aagtttaaaa tgtattttgg ttaactctct tcggatcaat tggctcgtct
      661 cttctcgcaa cagattaaac tgattttcaa aactttcaat gaatcgttca ttcaaccatt
      721 cgattgtttc atcttcattt tttctcaagt atttttgatc caatttatca tgaagataaa
      781 gccaagagag tgtcttattg ataaaaataa cggatttttc tcctaatact ccttttaagt
      841 ggtaaaatcc tatatccgaa aaaaatagcg actgattgct agcaatatct aaagaatcag
      901 cttgttcagc caacaggttg attgctaaaa agtggaataa tttctttttt tcataagcag
      961 acagaggata atactgtttt aaatctttta acattttttc tgtttttata attataggta
     1021 gcgtatacaa ttccacaaaa cttatctctg ttgagttaaa aagattatat aaaaaaagcc
     1081 gaatgttttt aggattccct gtcaa
//
LOCUS       DAZUMO010000194         1097 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000194 DAZUMO010000000
VERSION     DAZUMO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.204"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1032
                     /locus_tag="ACG8ER_002966"
     CDS             <1..1032
                     /locus_tag="ACG8ER_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFS5051456.1"
                     /translation="TRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRK
                     LIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGT
                     THQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTD
                     RFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHY
                     HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLP
                     QSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIY
                     LQQGLIFEN"
BASE COUNT          337 a          240 c          211 g          309 t
ORIGIN      
        1 acccgttttc tctgccatga ttgtggaagg accttttctg cgaaaacgga cttagttgat
       61 gaacatcatc aactaacaaa ggaactgaag caggcaattc tcatggaact gtacgaaaat
      121 caatcacgta aactaatcgc taagaagtac ttcgtctcgg atgggacggt gacacggatc
      181 ttgcgtgaag caacgaagca ttaccagtca cgtatgaact tccttccgac ggtgttgtgt
      241 atggatgagt tcaaatcaat gaagtctgtt tctggttcca tgagttttat ttgtgttgat
      301 gggacaaccc atcagctatt tacgatcctt gaagatcgca gactttacaa actaactcaa
      361 tacttcctgc actttcctag aaaagcccgt ttaaaggtca agtacctagt gatggacatg
      421 aatgccagct attgtcagtt gcttaaaaca gtctttccaa atgcggagat cgtaactgac
      481 cgctttcata ttgtccaaca catcaatcgc agcttcaatc aattacgtgt ccagatcatg
      541 aatcgttttc gaacctcaca ctctgaaaat cagaagaagt accgtcggct caagcgatat
      601 tggaaactct tactcaaaga ttccacgaca tgggagccac tcaaacggca ttatcatcga
      661 ctgtttaaac gaccaattag tcaaacagaa atcgttgatg agctgttaag ctacaacgaa
      721 gaattgcgaa ctgcgtatca tttttgtcaa ttattacgct attatttcgt taaacgaggt
      781 acggaaggct ttcaagaaac ccttaaaaca atttcatcta cgttacctca gtcgtttaaa
      841 aagaaattca cgatcttcca ttgctaccaa tcgggtattt caaatgcctt taaggtgtcc
      901 cactcaaacg gcgtcactga ggggctgaat aacaagatca agctgattaa acgaattgcg
      961 tttggttatc gcaactttta taactttcgg gcacggatct atttacaaca aggtctgatc
     1021 tttgaaaact aaaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat
     1081 tcaaatttgt cttcacc
//
LOCUS       DAZUMO010000195         1096 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000195 DAZUMO010000000
VERSION     DAZUMO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.205"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..238)
                     /locus_tag="ACG8ER_002967"
     CDS             complement(113..238)
                     /locus_tag="ACG8ER_002967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051457.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMVTRCCLLALVIAAANA"
     gene            628..>1096
                     /locus_tag="ACG8ER_002968"
     CDS             628..>1096
                     /locus_tag="ACG8ER_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5051458.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDK"
BASE COUNT          393 a          188 c          211 g          304 t
ORIGIN      
        1 tatccataaa actaatataa agcttggtac gattctgttt gtaagacata cgaatagtat
       61 cagaggtagg agccaagctg cctgatatta aattatcgat aaagtagaaa ggttaagcat
      121 tagccgctgc aataactaat gctaggagac agcaacgtgt aaccattacc gcacgtgttt
      181 ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga aatacggtgc aaatgcacgg
      241 tggtttcatg caatgcttac agggcgtatt ttgtaagggt atgtaacgga gataattccg
      301 acggtaatgg atgtgagagg ccaagccgac gctgacggta gggcaaacaa acatagttgc
      361 actctagcga tcgttattta tttttaacgg tcaaactagg gtacaactat gcttaaaaca
      421 cttcgcctca acttctggta gcaaaagtga ttttgaaagt caaaatattt tttatgagtt
      481 atttacaatt ctaaaaaaca agtgtattaa tctactaatt gagagtataa aatattttgt
      541 gtaaatgaaa aaatccatac aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa
      601 ccaattcaca gaaaagagga cttccctatg aatgatttta ctacagaaat tgtgcaaact
      661 ctagtcacta aaggcgattt aaatgaatta ttccgttcgc acttagaaaa agcgataaac
      721 acactcctac ggactgaatt aacggctttt ttagattacg aaaaatatga tcgcactggt
      781 tttaattcag gtaattcgag aaacggttct tactttcgat caatcaaaac cgaatatggt
      841 gaattaacat tggaaatacc tagagatcgt aatggtgagt ttaaacaaca aactttacca
      901 gcctacaaaa gaacaaacga tacattggaa accactatta tccatttatt cgaaaaaggt
      961 gttacgatgt ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca
     1021 caaaccatgt ccaacatgac taaagttctg actgaagaag taaatgcctt taaatccaga
     1081 gccttaaatg ataagt
//
LOCUS       DAZUMO010000196         1073 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000196 DAZUMO010000000
VERSION     DAZUMO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.206"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8ER_002969"
     rRNA            <1..>1073
                     /locus_tag="ACG8ER_002969"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZUMO010000197          989 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000197 DAZUMO010000000
VERSION     DAZUMO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.207"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>989)
                     /locus_tag="ACG8ER_002970"
     CDS             complement(<1..>989)
                     /locus_tag="ACG8ER_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5051459.1"
                     /translation="FPIYNIYLHFYKASYNYHLIDFMYKYPRSVLKDFAKEQFTSVST
                     VFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYYFYWNSTRESSDKWPFHIEQ
                     KEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSFRKVRVDNEYKSVISDDPHF
                     NLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYENSHAYAHQRQNTCSYRAVE
                     NLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNPDLFFNRSYIEEMKEEEPRT
                     YHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDYYDLFLTNVKPIKIL"
BASE COUNT          372 a          154 c          121 g          342 t
ORIGIN      
        1 taaaatctta atcggtttaa catttgttaa aaataaatca taataatcta acacataata
       61 ataatccaaa aataattgtg cccagttttc taatttcttt gatacatcca aatccgcatt
      121 tgcttgtaat tcttttttga gtttttccat tatgtgataa gttcgaggtt cttcttcttt
      181 catttcttcg atatacgaac gattaaaaaa aagatctgga ttaccataaa atagataact
      241 tctttcatga aatgcaataa aattgaatat gagttctgga tcctttatat ctaaagaaaa
      301 acggaacatg ctttgtaaaa ctttctccaa attctcaact gctcgataag aacaagtatt
      361 ttgtcgctga tgtgcatatg catgactatt ttcatatatt gcatttccat caattactcc
      421 aaaagaataa attattcgat aaaggaaaca cagttcatcg ctgttaaaag aaagattgat
      481 ttgttttgac cacttcttca aaagattaaa atgcggatca tctgagataa ctgatttata
      541 ttcattatct actcgtactt ttctaaatga acttctttct atattaatgg ctaaccaaaa
      601 agaaaataca cgtttttgaa aaatagttaa cgtaatatca taaatcccct caaaagcaac
      661 tatatacttc tctatttcct tttgttcgat atgaaatggc cacttatcac tactttcacg
      721 tgtagaattc caatagaaat aataaaaaaa gctacgaatg tttgcctctg atgcattcaa
      781 ttctaactga aatggatgaa atgttatatg ataacgccta aaatatggaa ttaagagctt
      841 cgcatatctg aaaacagtag atacactagt aaattgctct ttagcaaaat cttttagaac
      901 acttctagga tatttataca taaaatcaat caaatgataa ttataagaag ctttataaaa
      961 atgaagataa atattataaa taggaaaat
//
LOCUS       DAZUMO010000198          918 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000198 DAZUMO010000000
VERSION     DAZUMO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.208"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG8ER_002971"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG8ER_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5051460.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          266 a          190 c          139 g          323 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaa
//
LOCUS       DAZUMO010000199          908 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000199 DAZUMO010000000
VERSION     DAZUMO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.209"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..875
                     /locus_tag="ACG8ER_002972"
     CDS             174..875
                     /locus_tag="ACG8ER_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5051461.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          329 a          159 c          146 g          274 t
ORIGIN      
        1 aaatcattcg cttcaaaaca aattattcaa taccattgta tattttttac ataataacaa
       61 attatatttg taaagtatta aaacttgttt ctaatgttgg tatgttaaaa ctttacaaat
      121 tctttaaaaa aagtgacaat aaatatagtg aacaataagg agaggacttt ttaatgcgtg
      181 ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt gaacatttct
      241 cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat aaaaaaggac
      301 agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc aaaggactcg
      361 ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat gtgaatacaa
      421 ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat acagctcaag
      481 cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa
      541 aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt gaaatacatg
      601 aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa caaaatttat
      661 tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc attccttatc
      721 cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact gctggacaag
      781 taatcaaaca actacgtgat tccggactgt tagattatga caaaaagcgt tttactttct
      841 gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa gcagtgaccc
      901 tgatattt
//
LOCUS       DAZUMO010000200          906 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000200 DAZUMO010000000
VERSION     DAZUMO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.210"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..286
                     /locus_tag="ACG8ER_002973"
     CDS             <1..286
                     /locus_tag="ACG8ER_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFS5051462.1"
                     /translation="LFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEE
                     NLDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            279..626
                     /locus_tag="ACG8ER_002974"
     CDS             279..626
                     /locus_tag="ACG8ER_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051463.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
     gene            623..805
                     /locus_tag="ACG8ER_002975"
     CDS             623..805
                     /locus_tag="ACG8ER_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051464.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT          343 a          106 c          176 g          281 t
ORIGIN      
        1 tcttttttta ttcaataagt tttatgaggg gcaggaagta cgacatatag gagtaactta
       61 tagcagatta gtatacacag attccctaca attagattta tttagtgatc caagaaaaaa
      121 gattgctgaa gaaaatttgg ataggattat tgataaaatt agacaaaaat atggatttac
      181 agctattata catgcttcat ctatgttaga aggtgcaaga agtattacga gaagcacttt
      241 aataggagga cacgcaggag gattaggagg tttgcataat gattgaatat aacgatagag
      301 gacgtttaaa atggtcggga ttttatttgt ctgatcatac tgaaagaatc aatgcagaca
      361 atgtacaacg tgctaatcat aatttggcaa aagaacaaat gacgactgaa gagattgcga
      421 aaatactgag aactgctgtc ataaaaaata gacccgtctg cattcaaaaa gaggaacgaa
      481 acgctgaagg ttactatcca gatgatgtag taggaaaaat tattggatat gacgagcttg
      541 gtgtatttgt ggaagaagaa aaagttcact atgatgaaat taggaatgtt tctttcgcgg
      601 aattattaaa atggagtcag atatgaacat tattaaaaaa tacgaattcg gttatattac
      661 gtatgaacag ctaatagagg aattatgggg atatggtcaa caattaatta atgaagtggg
      721 gattgattgc ttttgtttct atattgaatc cggagcagga taccataaga acagatacta
      781 tatatttcca tatccaccaa aatagtctct tagagatttt taaattttct ccaaatactt
      841 tcaattattg tgaaatattt ccttttaatt aatcaaattt tttataaaag taatatataa
      901 aataaa
//
LOCUS       DAZUMO010000201          867 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000201 DAZUMO010000000
VERSION     DAZUMO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.212"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..>867
                     /locus_tag="ACG8ER_002976"
     CDS             81..>867
                     /locus_tag="ACG8ER_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFS5051465.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNI"
BASE COUNT          321 a          160 c          161 g          225 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag tggcagaatc
      541 gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat ataaaagtac
      601 gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata accaaagatg
      661 gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagcgaagag acctggacaa
      721 cattttttga atacctaaaa gaacgcggtt tacaaggtac ggaactcgtt atttctgatg
      781 cgcacaaagg attagtctct gccattagaa aatccttcac caacgtaagt tggcaaagat
      841 gccaagttca cttcctaaga aatatct
//
LOCUS       DAZUMO010000202          847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000202 DAZUMO010000000
VERSION     DAZUMO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.213"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..644
                     /locus_tag="ACG8ER_002977"
     CDS             69..644
                     /locus_tag="ACG8ER_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS5051466.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(713..>847)
                     /locus_tag="ACG8ER_002978"
     CDS             complement(713..>847)
                     /locus_tag="ACG8ER_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5051467.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          236 a          144 c          181 g          286 t
ORIGIN      
        1 cgttctttta aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg
       61 ctaacaaaat gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta
      121 ttgggatcaa ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg
      181 attctatcgg aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag
      241 gattgtccag cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc
      301 caatccaaat tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag
      361 ataaaaacag cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg
      421 tttcttcgat cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga
      481 tcgttcagct attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg
      541 cgggtacaga ctaccttgat cgcttgttgt ctatcgtcgt tgtttcatta gtcatcacta
      601 tgttgcccca acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat
      661 tagcaaaata atgctaatct ggctctgttt tttcatttta ttatttgtca aattaagcga
      721 gacaaaactt tccatttacg taactcaaaa aaacttctaa tggtgtttgg taatttagtg
      781 attttctagg gatatggttt cttttggacg caacggatga gatgaatcct tgattgactt
      841 ggttgaa
//
LOCUS       DAZUMO010000203          802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000203 DAZUMO010000000
VERSION     DAZUMO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.214"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>802
                     /locus_tag="ACG8ER_002979"
     CDS             <1..>802
                     /locus_tag="ACG8ER_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5051468.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSW"
BASE COUNT          272 a          152 c          150 g          228 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aa
//
LOCUS       DAZUMO010000204          788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000204 DAZUMO010000000
VERSION     DAZUMO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.215"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..688
                     /locus_tag="ACG8ER_002980"
     CDS             <1..688
                     /locus_tag="ACG8ER_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS5051469.1"
                     /translation="TYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWN
                     ELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDR
                     KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIR
                     RTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQV
                     TDTLVSMFTE"
BASE COUNT          288 a          144 c          129 g          227 t
ORIGIN      
        1 tacttacatt ccactaaaac gtcaaaccgt atccaaagaa gcgatttata ttgccattgg
       61 tatacgagaa gacggcacta aagaagtact gagttatgcg attgctccaa ctgaatcaac
      121 atacgtttgg aatgagctgc tacaggatat taactccaga ggagttcaag aagtcttgct
      181 ttttattacg gacggcttaa aaggcatgaa agatactatc catcaaattt atcctaaagc
      241 aaaatatcag cattgttgta tccatgtatc tcgtaacatc gctcataaag tacgtgtcaa
      301 agaccgaaaa gaaatctgtg atgactttaa ggctgtttat caagctaact caaaagaaga
      361 agcgaatacc ttcttatccg gcatgattga gaaatggaag aaaaactatc ctaaagtgac
      421 gcagtcactc atagaaaacc aagacttatt aactttttat gattttccac ctagcattcg
      481 tagaaccatt tactcaacca atctaatcga gtctttcaat aagcaaatta aaagatacag
      541 ccgtagaaaa gagcagtttc aaaatgaaga atcactagaa cgctttctag tcagcatttt
      601 tgatacatac aatcaaaaat ttctaaacag aagccataaa ggttttcaac aggtaaccga
      661 tacattagtt tcaatgttta ctgagtaact aattattttg caggaggaca atttatttac
      721 acaaaattat tgacgctccc aattaggact caattgttca tagatgagca tttgtaatta
      781 agttatat
//
LOCUS       DAZUMO010000205          753 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000205 DAZUMO010000000
VERSION     DAZUMO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.216"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            219..617
                     /gene="spxA"
                     /locus_tag="ACG8ER_002981"
     CDS             219..617
                     /gene="spxA"
                     /locus_tag="ACG8ER_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS5051470.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          266 a          108 c          144 g          235 t
ORIGIN      
        1 tgaattagat ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta
       61 ctgtatatta accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct
      121 atttaaaagt tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac
      181 aaaaaagtga ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc
      241 caagttgtac ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg
      301 ttgaacgtaa tattttttca gaaccattga acagttctga attaaaagcg attttacaaa
      361 tgacagaaga cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga
      421 acatggattt agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg
      481 gattgcttcg ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag
      541 atgaaattcg tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat
      601 tgatggctgg attatagaag gataatacga aacttggaaa agactggctc tttgtcaaat
      661 gatgttggta aagagaaatc ttagaataat ttggacagaa cagaaagaag aaaacctctt
      721 tctgttctgt ttatttggtt aaaaatgttt tgc
//
LOCUS       DAZUMO010000206          746 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000206 DAZUMO010000000
VERSION     DAZUMO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.217"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(428..652)
                     /locus_tag="ACG8ER_002982"
     CDS             complement(428..652)
                     /locus_tag="ACG8ER_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051471.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          277 a          105 c          133 g          231 t
ORIGIN      
        1 aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta ttcgtttatt
       61 gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc
      121 tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct acttgagatt
      181 ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc
      241 gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac
      301 agtacctctt tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt
      361 gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct
      421 gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata
      481 aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca
      541 tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc
      601 gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt
      661 catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta
      721 cgttttttag aataaaaatt ataagg
//
LOCUS       DAZUMO010000207          732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000207 DAZUMO010000000
VERSION     DAZUMO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.218"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>732)
                     /locus_tag="ACG8ER_002983"
     CDS             complement(109..>732)
                     /locus_tag="ACG8ER_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFS5051472.1"
                     /translation="STYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYV
                     RVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKE
                     FDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLEL
                     FDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          207 a          166 c          138 g          221 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta ga
//
LOCUS       DAZUMO010000208          640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000208 DAZUMO010000000
VERSION     DAZUMO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.220"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..283
                     /locus_tag="ACG8ER_002984"
     CDS             <1..283
                     /locus_tag="ACG8ER_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5051473.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          214 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt
//
LOCUS       DAZUMO010000209          630 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000209 DAZUMO010000000
VERSION     DAZUMO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.221"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..586
                     /locus_tag="ACG8ER_002985"
     CDS             <1..586
                     /locus_tag="ACG8ER_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5051474.1"
                     /translation="PLASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQIL
                     SVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKII
                     LSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNG
                     LGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          279 a           40 c           90 g          221 t
ORIGIN      
        1 ccctcttgct agtcttaaaa taatactgga aaacatgaaa tacaatattg gaaaatataa
       61 agaaagagat atttatatta atgaatgtat tgaaatcgtt gatagtttaa caaaaaatat
      121 ttctcaaata ttatcagttc attctataga aaatttgaat aatgatgaag aatatttgaa
      181 aataaatgat acattagaag acatattaaa aaagtatgaa atattagcaa atcaaaagaa
      241 aataactgtc aataattata tattagacga gaatgtttat ataggaaaaa cagctttaaa
      301 gattattcta tctaatttga ttagtaatgc ggtaaaatat actgatgtaa atggggtaat
      361 taatattggg atagttaatg attggttata tatagagaat tcatacggta acaataaaat
      421 ttcgaatatg gataaaatat ttgatgttaa atttgattta aataaggaaa atagtaatgg
      481 attaggttta tatattgtaa gtaatattct gaataattac aatatagaat ataaagtatt
      541 gcaggatgaa gaatttttta tctttaaaat aaaaatattt tcttaattaa gatataattt
      601 tgcaatagtt attgaaaaat agagacttat
//
LOCUS       DAZUMO010000210          630 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000210 DAZUMO010000000
VERSION     DAZUMO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.222"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>630
                     /locus_tag="ACG8ER_002986"
     rRNA            <1..>630
                     /locus_tag="ACG8ER_002986"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DAZUMO010000211          593 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000211 DAZUMO010000000
VERSION     DAZUMO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.223"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACG8ER_002987"
     CDS             <1..525
                     /locus_tag="ACG8ER_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS5051475.1"
                     /translation="SEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
BASE COUNT          228 a           91 c          139 g          135 t
ORIGIN      
        1 tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagcgg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagagaataa gaatattgtg
      241 cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa tcaagtgatt
      361 ttaaaaggaa aaagttatgg aaaagagatt caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatc cattccgatt ttcacttgaa acagtgtcag aagtggccaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt tgaaaatgac cga
//
LOCUS       DAZUMO010000212          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000212 DAZUMO010000000
VERSION     DAZUMO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.224"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG8ER_002988"
     CDS             154..417
                     /locus_tag="ACG8ER_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5051476.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>588
                     /locus_tag="ACG8ER_002989"
     CDS             417..>588
                     /locus_tag="ACG8ER_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5051477.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRY"
BASE COUNT          223 a           78 c          119 g          168 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtatt
//
LOCUS       DAZUMO010000213          546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000213 DAZUMO010000000
VERSION     DAZUMO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.225"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..546
                     /locus_tag="ACG8ER_002990"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACG8ER_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          215 a           94 c          103 g          134 t
ORIGIN      
        1 tacgcctgaa caaaaagaat tgttagaact acgtaaaaga aaccagcaat tagaaatgga
       61 aaatgatatt ttaaagcaag cagcgctgat attcggacga agagacaagt aatcgatgcg
      121 aataagcatc tttaccctat atcagcgatg tgcagaatat taggtctatc acgtcagtcc
      181 tattattatc aatcaaaacc aaagaaagac gaatcagaac ttgaagaagt agtcgctgaa
      241 gaatttatcc gcagccgaaa ggcctacggc tcaagaaaaa taaaaaaagc cttatcaaaa
      301 cgaggcattc agatcagccg acgaaaaatt agtagaatca tgaaaaatag aggattaaaa
      361 tcgagctata ctgttgctta ttttaaagta catcattcta cttgcaatga agccaaaacg
      421 acaaacgtat tgaatcgtaa attcttaaga gacaacccat tagaagcgat cgtaacagac
      481 ttgacttatg tacgagtcgg gaaaaaatgg aattatgtct gtttcatttt ggatctgttc
      541 aatcga
//
LOCUS       DAZUMO010000214          514 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000214 DAZUMO010000000
VERSION     DAZUMO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.226"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8ER_002991"
     CDS             complement(<1..132)
                     /locus_tag="ACG8ER_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5051478.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZUMO010000215          488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000215 DAZUMO010000000
VERSION     DAZUMO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.227"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>488)
                     /locus_tag="ACG8ER_002992"
     CDS             complement(<1..>488)
                     /locus_tag="ACG8ER_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051479.1"
                     /translation="VFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFS
                     LLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRY
                     NYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKP
                     FF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DAZUMO010000216          473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000216 DAZUMO010000000
VERSION     DAZUMO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.228"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>473
                     /locus_tag="ACG8ER_002993"
     CDS             <1..>473
                     /locus_tag="ACG8ER_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5051480.1"
                     /translation="TAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPE
                     EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPK
                     DTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          192 a           71 c          112 g           98 t
ORIGIN      
        1 accgcaggag aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacggc
       61 gtaagcttac cagaagataa acttccagtg ttgaaagcaa aagatggtta tacagatgcg
      121 aaatggccag aggaagcaac tcaaccaatt acagcagatg atacagaatt tgtatcaagt
      181 gcaacaaaac tggatgataa atctgacgct gacaaatata atcctgaagg tcaaaaagtg
      241 actacagaat tgaataagga accagacgca tttgagggaa ttaagaacaa agaagattta
      301 ccaaaagata ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa
      361 aaagggactg ttgtagtgac atatccagat ggatcatctg atgaagttga agtagatgtt
      421 acagtgacag acaatcgttc tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DAZUMO010000217          472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000217 DAZUMO010000000
VERSION     DAZUMO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.229"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          189 a           58 c           67 g          158 t
ORIGIN      
        1 gatcaattta tatccttaaa aaacattata ataaatgttt tttatatcgt tatttattga
       61 tattaatatc atataaaacg atgaaagcgc atacttttaa gaggggttgt atgtaatttt
      121 aatacctata ccaatttaaa gaggattttt cagattttca cttacaaatt tataaaaaaa
      181 caatataatt gatactttga ttttgcgaac attttgcctt tccgcctaat tcagctttaa
      241 taccttgatt atatcagtaa ctaatcgcct ttttagctaa tttaagcatc aaataattat
      301 ccttaatcag ggtaaaaaat aacgaataaa tggatttttg gaataggtaa aaactagatc
      361 ggtttctgac gaaaaataac attaaaaagg aaatataaaa taacatggag cgtgggagga
      421 ataaagaaat tttacacaca aggggcgtag gaatgtaaag aaacttaaca ca
//
LOCUS       DAZUMO010000218          460 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000218 DAZUMO010000000
VERSION     DAZUMO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.230"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>460)
                     /locus_tag="ACG8ER_002994"
     CDS             complement(<1..>460)
                     /locus_tag="ACG8ER_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051481.1"
                     /translation="NQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEH
                     IIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQ
                     VNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHK"
BASE COUNT          141 a           81 c           65 g          173 t
ORIGIN      
        1 cttttatgga aaactatttt aaaaaataaa cataaatatg cagttaatgt aacaagataa
       61 gaaataatag tcgttactgt ataacttgtg cttactttaa tatcagcaac tcctttgact
      121 ccgcttattt gtaccaatcc attagcattc acctgctcct ttacaggttg atgatttatt
      181 tctacattcg ttcctaaata agcatataca ggaagtgtga ttacttggct cttctcatta
      241 gttactctat atatgataga actaccagtt gttttataag gaaggtttac ccattgactg
      301 ccaatctttg cttcgtgttt tattatatgc tccttattct ccaatgaagc gataggagaa
      361 taatcatata aaaaataatc cattccttta gtggtatacg cctctactgt ttctttgttt
      421 acaacaatat gtcccggctg agaaattatt ttttgattga
//
LOCUS       DAZUMO010000219          446 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000219 DAZUMO010000000
VERSION     DAZUMO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.231"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          139 a           87 c           52 g          168 t
ORIGIN      
        1 aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga tctcattctt
       61 tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt atatattcgt
      121 attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg tccgttactt
      181 aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt ttcattaatt
      241 gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc aaactcattc
      301 attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac atcactttat
      361 tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat acactactat
      421 tcagcccatt caacgaccaa tgaatt
//
LOCUS       DAZUMO010000220          421 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000220 DAZUMO010000000
VERSION     DAZUMO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.233"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>421
                     /locus_tag="ACG8ER_002995"
     CDS             <1..>421
                     /locus_tag="ACG8ER_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS5051482.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFI"
BASE COUNT          154 a           73 c           87 g          107 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 c
//
LOCUS       DAZUMO010000221          390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000221 DAZUMO010000000
VERSION     DAZUMO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.234"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACG8ER_002996"
     CDS             <1..379
                     /locus_tag="ACG8ER_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS5051483.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          135 a           77 c           77 g          101 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac
//
LOCUS       DAZUMO010000222          382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000222 DAZUMO010000000
VERSION     DAZUMO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.235"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>382
                     /locus_tag="ACG8ER_002997"
     CDS             <1..>382
                     /locus_tag="ACG8ER_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5051484.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIV"
BASE COUNT          140 a           68 c           76 g           98 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tg
//
LOCUS       DAZUMO010000223          381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000223 DAZUMO010000000
VERSION     DAZUMO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.236"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..364
                     /locus_tag="ACG8ER_002998"
     CDS             89..364
                     /locus_tag="ACG8ER_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051485.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          163 a           57 c           53 g          108 t
ORIGIN      
        1 tttatatata tttcagtatc cttcttgttt tcatataacc aaaggcgcta tgatttccat
       61 atgaaaaaaa taaagtcagg tgaaaaaaat gaaacttgag gataaaaaag tcattttgaa
      121 gaaaatcaaa cgactaccag accaaataga tggctatacg atctgttctt tcaaaaaaga
      181 caggagtata aaagcttata cgcaaaacac agaaaaagga ataatctatt tcctacatga
      241 agaaggttac gatacccaaa cctttatgtt tactgaaaaa aaagaatttc ataaacagat
      301 gaaaaaactg attgaaaaag aatttccaag aagtcataaa ctctatgtga atcagcaatt
      361 ttaacttcta actgttcttt a
//
LOCUS       DAZUMO010000224          380 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000224 DAZUMO010000000
VERSION     DAZUMO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.237"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>380
                     /locus_tag="ACG8ER_002999"
     CDS             <1..>380
                     /locus_tag="ACG8ER_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS5051486.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHK"
BASE COUNT          133 a           71 c           62 g          114 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa
//
LOCUS       DAZUMO010000225          328 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000225 DAZUMO010000000
VERSION     DAZUMO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.238"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          136 a           43 c           57 g           92 t
ORIGIN      
        1 cttttaaaaa ttgtatttga catgaattta cgacaaggaa ttagtgggga ttaagcgatt
       61 attctcttcc actttagaga aataatgggt taaaaagcca gaatacgtag agcaatattg
      121 acaacctata aaggagctat tgttgaaaat tgaagtaaca aaaagataaa gagaggttta
      181 catctatcag attaaaagtg atagtacacc catgaaacct gcaaaagagt tgaacctaat
      241 gtatatcggt ttaactcttt ttatttgcaa attaaaaaaa tatcaccgaa gtgacgaatg
      301 aaactatgac caaattaagc aagaaaat
//
LOCUS       DAZUMO010000226          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000226 DAZUMO010000000
VERSION     DAZUMO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.239"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8ER_003000"
     rRNA            complement(<1..>321)
                     /locus_tag="ACG8ER_003000"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           63 a           96 c           74 g           88 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat g
//
LOCUS       DAZUMO010000227          309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000227 DAZUMO010000000
VERSION     DAZUMO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.240"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACG8ER_003001"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACG8ER_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAZUMO010000228          305 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000228 DAZUMO010000000
VERSION     DAZUMO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.241"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8ER_003002"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8ER_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS5051487.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAZUMO010000229          299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000229 DAZUMO010000000
VERSION     DAZUMO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.242"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACG8ER_003003"
     CDS             <1..>299
                     /locus_tag="ACG8ER_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5051488.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZUMO010000230          290 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000230 DAZUMO010000000
VERSION     DAZUMO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.244"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..>290
                     /locus_tag="ACG8ER_003004"
     CDS             37..>290
                     /locus_tag="ACG8ER_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5051489.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           88 a           53 c           57 g           92 t
ORIGIN      
        1 atagatttat gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat
       61 ggtagaaatc aatttgcgat gcttacgatt gatgatctag ttcctcaaga tcatctcgtt
      121 cgaaagattg atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac
      181 tccgatttag gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag
      241 tatttattcg gtatccgttc catgcgacaa accattaagg aagtcgatac
//
LOCUS       DAZUMO010000231          286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000231 DAZUMO010000000
VERSION     DAZUMO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.245"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>286
                     /locus_tag="ACG8ER_003005"
     CDS             28..>286
                     /locus_tag="ACG8ER_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5051490.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          108 a           39 c           58 g           81 t
ORIGIN      
        1 tagaaaaaga tagcaggagg aatgacgatg aatcatttta aaggaaagca atttcagcag
       61 gatgtgatta ttgtagccgt gggctactat cttcgttata accttagcta tcgtgaagtt
      121 caagaaatct tatatgatcg tggcattaac gtttctcata cgacgattta tcgttgggtg
      181 caagaatatg gcaaactact ctatcaaatt tggaaaaaga aaaataaaaa atccttttat
      241 tcatggaaaa tggatgaaac gtacatcaaa attaaaggaa aatggc
//
LOCUS       DAZUMO010000232          267 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000232 DAZUMO010000000
VERSION     DAZUMO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.246"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          112 a           28 c           51 g           76 t
ORIGIN      
        1 aaggtgtcac taaaaattat aaaaaatagg gttgacgcac ttttgatgtc atgttataat
       61 aacgaagtcg tttggatgag gtagctaata aagcttgatt ttatcgaaaa aaacttcgaa
      121 aaagttgttg acaaataaca ataaagatga tatgataatc aagtcgctgc taatgagtga
      181 caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg aatctgataa
      241 gattaaagag ttgttgaaac aaaaaga
//
LOCUS       DAZUMO010000233          265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000233 DAZUMO010000000
VERSION     DAZUMO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.247"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          107 a           42 c           36 g           80 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgt
//
LOCUS       DAZUMO010000234          237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000234 DAZUMO010000000
VERSION     DAZUMO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.248"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..216)
                     /locus_tag="ACG8ER_003006"
     CDS             complement(<1..216)
                     /locus_tag="ACG8ER_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5051491.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT           62 a           44 c           44 g           87 t
ORIGIN      
        1 aaaaatgatt tcggttctcc cacaacgtcg cttattttct ttgtattgtt caaagtattc
       61 aagtgctgtt ccaccacatt tgagaaagtt atagacattg taaattgttt ggcgtccacg
      121 tttaagctgc ttcgcaacga tagcaaccgg agtttcttga tgaaaatatg cttctatcat
      181 tacaagctcg tttggtgtaa gatgggtata agtcatttat gttcactctc cttgtat
//
LOCUS       DAZUMO010000235          235 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000235 DAZUMO010000000
VERSION     DAZUMO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.249"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>235)
                     /locus_tag="ACG8ER_003007"
     CDS             complement(<1..>235)
                     /locus_tag="ACG8ER_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5051492.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT           69 a           45 c           36 g           85 t
ORIGIN      
        1 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac
       61 actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt
      121 gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg tttttctttg
      181 ttttccaagt aagctgtggt ctagtttttc tgatttatcc atgtttttac gtggc
//
LOCUS       DAZUMO010000236          232 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000236 DAZUMO010000000
VERSION     DAZUMO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.denovo.250"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           73 t
ORIGIN      
        1 gagaaaaagc atggaatgcg ctgcatcagg tatcgaggct tagaaaaaaa tcaggtgcat
       61 acgatgcttg tttttgccga aatgagtctg aaaaaattgg tcacttagca gtgggagcat
      121 atgtcttcat attttttatt atacaaaaat agacaaaagt atcagtctac actcttagta
      181 aagaggaaac tgatactttt gtctacagta tgatatttct tacatctact tt
//
LOCUS       DAZUMO010000237         8569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.099, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000237 DAZUMO010000000
VERSION     DAZUMO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.099"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..2089
                     /locus_tag="ACG8ER_003008"
     CDS             182..2089
                     /locus_tag="ACG8ER_003008"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5051493.1"
                     /translation="MKEIQEQHSHENNSHDHGKMPIILYFIGLALSLIALFVNGENNL
                     IQNTLFSIASISAGYHVIILEGIGETIENSKRQHKFTPNSHILMGIAAIGASLIGNFW
                     EGTLLVLIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLIMQDGNTTIVNVSELKVG
                     DQLQVLNGDQVPIDGVIVSGSTSIDESSINGESIPKEKSKGDGVFGSTINGTGTFTME
                     VTKETNDTVFSKILQLVNQNQENQTKASSIIQKFEPKYVKFVLIAIPLFILLAPMLFD
                     LTWSQSIYRGLVLLVAASPCALAAATVSVTLSATSNLAKRGVLSKGSAYLSQLADIQA
                     IAFDKTGTLTKGKPEVTNYYFSDSVNEENMIDIVVALEKESNHPLASAILDKFEPKNK
                     LAIEVENQIGKGLIGDYNENTYRIGKPTSFEAVSDDYTRLNKEWASEGNTVVYVSENE
                     KVIGLIALMDIPSEHAKATIDYFKKCGIHTTLITGDSEMTGQAVGKQLGVDQVIANVM
                     PEDKSNIINEQKETYGITAMVGDGINDAPALVKADVGIAMGEGTDVAVDVSDLVLMKN
                     DLSKLVKAHEISLKMNRVIWQNIIFSMVVVAFLITVSLVGLTDIAISVIVHEGSTLVV
                     ILNGLRLLKSK"
     gene            2183..2404
                     /gene="relB"
                     /locus_tag="ACG8ER_003009"
     CDS             2183..2404
                     /gene="relB"
                     /locus_tag="ACG8ER_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5051494.1"
                     /translation="MTVISLRIDTQEDKLIKEYAKAKNISVSALIRSSVLEKIEDEID
                     LGLYNQAMKDHKENPKSISFEDMLKELSD"
     gene            2405..2677
                     /locus_tag="ACG8ER_003010"
     CDS             2405..2677
                     /locus_tag="ACG8ER_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5051495.1"
                     /translation="MSSGYQVEFEKGAQKALKKMDKYQSLLIMGWIQKNLVNCTDPRQ
                     HGKGLTVNRSGEWRYRIGDYRLIADINDETVTILMLEIGHRKNIYK"
     gene            complement(2808..5774)
                     /locus_tag="ACG8ER_003011"
     CDS             complement(2808..5774)
                     /locus_tag="ACG8ER_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS5051496.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            5920..6495
                     /locus_tag="ACG8ER_003012"
     CDS             5920..6495
                     /locus_tag="ACG8ER_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5051497.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            6709..7404
                     /gene="vanR-A"
                     /locus_tag="ACG8ER_003013"
     CDS             6709..7404
                     /gene="vanR-A"
                     /locus_tag="ACG8ER_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5051498.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            7382..8551
                     /gene="vanS"
                     /locus_tag="ACG8ER_003014"
     CDS             7382..8551
                     /gene="vanS"
                     /locus_tag="ACG8ER_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFS5051499.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRGSVAKF"
BASE COUNT         2777 a         1491 c         1651 g         2650 t
ORIGIN      
        1 tgtataaggg tagctatttt agagtcctat tttttttatc acctaaataa gataactatt
       61 tttttgacaa attagataga gcgatataaa atgaatgtat agacatatga atgttcgttc
      121 aaacgtaatt cgtaagcatt acgatttcta aaaaaaagtt ttattaagaa aggtgataga
      181 aatgaaagag attcaagagc aacactcaca cgaaaataat agtcacgatc atgggaaaat
      241 gccaattatt ttgtacttta ttggtttagc attgtcgctc attgcgttat ttgtaaacgg
      301 agaaaataat ttaatccaaa acactttgtt ttcaattgct tcaattagtg ctggttatca
      361 tgtcattatt cttgaaggaa ttggagaaac gattgaaaac tcaaaaagac aacacaaatt
      421 tacccctaat tctcatattt taatgggaat agctgcaata ggtgcttctc tgataggaaa
      481 tttttgggaa ggaacgttat tggttcttat tttctctgga gcacattttc ttgaagatta
      541 tgctgaagga agaagtaaaa gagaaattac caagttatta gaaatgaatc caacaacagc
      601 taggctaatc atgcaagatg gcaatacaac aatcgttaat gttagtgagt tgaaagtagg
      661 agatcaactt caagtgttaa acggtgacca agtaccgatt gacggagtaa tagtatcagg
      721 ctctacttcg attgacgaat cttctattaa tggtgagagt atacctaaag agaaatcaaa
      781 gggagacgga gtttttggga gtacaattaa tggaacgggt acctttacta tggaagtcac
      841 aaaagaaact aacgatactg tcttttcaaa gattttacaa ctagtgaatc aaaatcaaga
      901 gaatcaaaca aaagcgtcta gtattatcca aaagtttgag cctaagtatg ttaaatttgt
      961 tttaattgca atcccactct tcattttact tgctcctatg ctgtttgatt tgacatggtc
     1021 acaaagtatt tatagaggat tagtcctttt agtcgcagct tctccgtgtg ctttagcagc
     1081 agctaccgta tccgtaacat tgtcagccac ttctaatcta gcaaaaagag gcgtactttc
     1141 aaaaggaagt gcctatttgt cccaattagc ggatattcaa gccattgcct ttgataaaac
     1201 aggaacatta actaaaggga aaccggaagt aaccaattat tacttttctg attctgtgaa
     1261 cgaggaaaat atgattgata tcgtagtggc tcttgaaaaa gaatcaaatc acccgttagc
     1321 aagtgctatt ttagacaagt ttgaaccaaa aaataaacta gctattgaag tggaaaatca
     1381 gattggaaaa ggtttaatag gcgattataa cgaaaacact tatcgtatag gtaaaccaac
     1441 ttcttttgag gctgtatcgg atgactatac acgtttgaat aaagaatggg cttcagaagg
     1501 taatacggtt gtatatgtat cagaaaatga aaaagtaatc ggcctgatag cattaatgga
     1561 tattccaagt gaacatgcga aagcaactat agattacttt aagaagtgtg gtatccatac
     1621 cactttaatt actggggatt ctgaaatgac agggcaagct gttggtaaac aattaggagt
     1681 agatcaagta attgcaaacg tcatgccaga agataaatct aacattatca atgaacaaaa
     1741 agaaacctat ggtataaccg ctatggtggg agatggtata aacgacgcac ctgctcttgt
     1801 gaaggcagat gttggaattg ctatgggaga gggtacggat gttgcagtag atgtatctga
     1861 tctagtatta atgaaaaatg atttgtctaa attggtaaaa gctcatgaaa tttctttaaa
     1921 aatgaatcga gttatttggc aaaatattat tttctcaatg gtagttgtcg cttttctaat
     1981 taccgtaagc ttagtaggat taacggatat agccattagt gtgattgttc atgaaggaag
     2041 taccttagtc gtgatattaa atggtcttcg attattaaag tctaaataaa tacatgaaat
     2101 tggataaaat catgtatcta aagtttgcag cctctctaat tttgtattac aatgtatata
     2161 caaaaagaag ggggtgattc taatgactgt catatcgctc agaattgata ctcaggaaga
     2221 taaattaatt aaagaatatg ctaaggcgaa gaatatttcg gtttcggctc tgattagaag
     2281 ttctgtcttg gaaaaaatcg aggatgaaat tgatttgggc ctatataatc aagccatgaa
     2341 ggatcataaa gagaatccga aaagtatttc ttttgaagac atgctaaagg agctatctga
     2401 ttaaatgagc tcaggttatc aagtcgagtt tgaaaaagga gctcaaaaag cgttaaaaaa
     2461 aatggacaaa taccaatccc ttttaattat gggatggatt caaaagaatc tagtgaattg
     2521 taccgatcct aggcagcatg gaaaaggatt aaccgtcaat cgatcaggcg aatggcgcta
     2581 tagaatcggt gattatcgct taatagctga tatcaatgac gagaccgtaa ccattttaat
     2641 gttagagatt ggtcatagaa aaaatattta caaataaacc attcaaattg agtgcaagca
     2701 tgtttaataa aaaaggattc tactgctttt taaggggtag cgtcaggaaa atgcggattt
     2761 acaacgctaa gcctattttc ctgacgaatc cctcgttttt aacaacgtta agaaagtttt
     2821 agtggtctta aagaatttaa tgagactact ttctctgagt taaaatggta ttctcctagt
     2881 aaattaatat gttcccaacc taagggcgac atatggtgta acaaatcttc attaaagcta
     2941 cctgtccgtt ttttatattc aactgctgtt gttaggtgga gagtattcca aatacttata
     3001 gcattgataa ttatgtttaa agcactggct ctttgcaatt gatgctgtat ggtgcgttct
     3061 ctaagctcac cttgttttcc gaagaaaata gctcttgcca atccattcat ggcttctcct
     3121 ttattcaatc ctctttgtat ttttcttctt aatgattcat ccgatatata attcaaaata
     3181 aagatcgttt tttctattcg gcccatctca cgtaaggctg tagctaagct gttttgtctt
     3241 gaataggaac ctagcttccc cataataagg gatgctgaaa ctgttccctc ccttatagaa
     3301 tgagctaatc gcaaaacatc ctcataattt tctttaatga cctttgtatt tatttgtcca
     3361 cgtaaaatgg cttctagttt tggatactca cttgctttat ctatcgtaaa taattttgag
     3421 tccgataaat cccttattct tggggcaaat ttaaatccta ataaatgagt cagtccgaat
     3481 atttggtcag tgtaaccggc agtgtctgta taatgttcct ctatgtttag atccgtctca
     3541 tgatgtaaca aaccatccaa aacatgaatc gcatctcttg aattagtatg aataatcttt
     3601 gtgtagtaag aagagaattg atcacttgta aatcggtaga tggtggctcc ttttccagtt
     3661 ccataatgtg gatttgcatc tgcatgtagt gatgaaacac ctagctgcat tctcatacca
     3721 tctgacgaag atgttgtacc gtcgccccaa tagaaaggca attgtaattt atgatgaaag
     3781 tttactaata tggcttgggc tttattcatg gcatcttcat acatgcgcca ttgagataca
     3841 ttggctagtt gcttatatgt aagtccgggt gtggcttcgg ccatcttgct caagccaata
     3901 ttcattccca ttcctaaaag ggcagccatg ataatgattg tttcttcctt atctggtttt
     3961 cgattattgg aagcatgagt gaattgctca tgaaatcctg ttatatgggc cacatccatg
     4021 agtaaatcag ttaattttat tcttggtagc atctgataaa ggcttgcact aaattttttt
     4081 gcttcttctg gaacatcttt ttctaagcgt gcaagtgata gctttccttt ttcaagagaa
     4141 accccatcta acttattgga attggcagct aaccacttta acctttcatt aaagctgctg
     4201 gttctctccg ttatataatc ttcgaatgat aaactaactg ataatctcgt attccccttc
     4261 gattgattcc atgtatcttc cgaaaacaaa tattcctcaa aatccctata ttgtctgctg
     4321 ccaacaatgg aaacatctcc tgcccgaaca tgctcccgaa gttctgttaa aacagccatt
     4381 tcatagtaat gacgattaat tgttgtacca tcatcctcgt ataaatgtct tttccatcgt
     4441 tttgaaataa aatccacagg tgagtcatca ggcacttttc gctttccaga ttcgttcatt
     4501 cctcggataa tctcaacagc ttgtaaaagt ggctcatttg cctttgtaga atgaaattcc
     4561 aatactctta atagcgttgg cgtatatttt cttagtgaat aaaaccgttt ttgcagtaag
     4621 tctaaataat catagtcggc aggacgtgca agttcctgag cctcttctac tgaagagaca
     4681 aaggtattcc attcaataac cgattctaaa accttaaaaa cgtctaattt ttcctctctt
     4741 gctttaatta atgcttgtcc gatgttcgta aagtgtataa ctttctcatt tagcttttta
     4801 ccgttttgtt tctggatttc ctcttgagcc ttacgacctt ttgataacaa actaagtatt
     4861 tgcctatcat gaatttcaaa cgctttatcc gttagctcct gagtaagttg taataaatag
     4921 atggttaata tcgaataacg tttattttct tgaaagtcac ggaatgcata cggctcgtat
     4981 cttgagccta agcgagacag ctgcaacagg cggttacggt gcaaatgact aatttgcact
     5041 gtttctaaat ccattcctcg tatgtattcg agtcgttcta ttatttttag aaaagtttcg
     5101 ggtgaaggat gacccggtgg ctcttttaac caacccaata tcgttttatt ggattcggat
     5161 ggatgctgcg aggtaataat cccttcaagc ttttcttttt gctcatttgt tagagattta
     5221 ctaaccgtat taaatagctt cttttcagcc attgcccttg cttcccacac cattctttca
     5281 agtgtagtga tagcaggcag tataattttg ttttttctta gaaaatctat gcattcatgc
     5341 agtagatgaa tggcatcacc attttccaaa gctaattgat gaaggtactt aaatgtcatt
     5401 cgatattcac tcagggtaaa agttacaaag tcgtattcac ttcgaatttc tttcaaatga
     5461 tcccaaagtg tattttccct ttgaggataa tgatcaagcg aggatggact aacaccaatc
     5521 tgtttcgata tatattgtat gaccgaatct gggatgcttt tgatatgagt gtatggccaa
     5581 ccgggatacc gaagaacagc taattgaaca gcaaatccta aacggttttc ttccctcctt
     5641 cgcttattaa ctatttctaa atcccgtttg gaaaaagtga agtaggtccc cagtatccat
     5701 tcatcttcag ggatttgcat aaaagcctgt ctctgttccg gtgtaagcaa ttctctacct
     5761 ctcgcaattt tcattcagta tcattccatt tctgtatttt caatttatta gttcaattat
     5821 atatcaatag agtgtactct attgatacaa atgtagtaga ctgataaaat catagttaag
     5881 agcgtctcat aagacttgtc tcaaaaatga ggtgatattt tgcggaaaat cggttatatt
     5941 cgtgtcagtt cgactaacca gaatccttca agacaatttc agcagttgaa cgagatcgga
     6001 atggatatta tatatgaaga gaaagtttca ggagcaacaa aggatcgcga gcaacttcaa
     6061 aaagtgttag acgatttaca ggaagatgac atcatttatg ttacagactt aactcgaatc
     6121 actcgtagta cacaagatct atttgaatta atcgataaca tacgagataa aaaggcaagt
     6181 ttaaaatcac taaaagatac atggcttgat ttatcagaag ataatccata cagccaattc
     6241 ttaattactg taatggctgg tgttaaccaa ttagagcgag atcttattcg gatgagacaa
     6301 cgtgaaggga ttgaattggc taagaaagaa ggaaagttta aaggtcgatt aaagaagtat
     6361 cataaaaatc acgcaggaat gaattatgcg gtaaagctat ataaagaagg aaatatgact
     6421 gtaaatcaaa tttgtgaaat tactaatgta tctagggctt cattatacag gaaattatca
     6481 gaagtgaata attagccatt ctgtattccg ctaatgggca atatttttaa agaagaaaag
     6541 gaaactataa aatattaaca gcctcctagc gatgccgaaa agccctttga taaaaaaaga
     6601 atcatcatct taagaaattc ttagtcattt attatgtaaa tgcttataaa ttcggcccta
     6661 taatctgata aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa
     6721 atacttattg tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac
     6781 gagaattata cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag
     6841 tctgagattg accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc
     6901 tgtcaaaaaa taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca
     6961 gaggtagata aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt
     7021 cgcccactgg agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt
     7081 ggagtaaagg agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac
     7141 acccatgagt gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata
     7201 ctgcgaatcc tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag
     7261 atatggggcg acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat
     7321 ttgcgcgaaa aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg
     7381 gttggttata aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta
     7441 catgtatatc gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat
     7501 gatccgaggg aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa
     7561 tcacctggac gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat
     7621 ttatgtggcg attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc
     7681 aaaatacttt gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca
     7741 aattgagctt tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac
     7801 tctggaaaag cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat
     7861 gtacttggcg cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct
     7921 tgacgaggct ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga
     7981 caaagcgtat cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct
     8041 acaaacgata acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac
     8101 cgatgaattt tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga
     8161 ggatctgacc gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa
     8221 aaacgccgct gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg
     8281 ggatgtggtg tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc
     8341 catatttgaa aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg
     8401 acttggattg gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga
     8461 aagcaatgat aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga
     8521 taaaaggagg ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DAZUMO010000238         8496 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.101, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000238 DAZUMO010000000
VERSION     DAZUMO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.101"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(202..573)
                     /locus_tag="ACG8ER_003015"
     CDS             complement(202..573)
                     /locus_tag="ACG8ER_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5051500.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            718..903
                     /locus_tag="ACG8ER_003016"
     CDS             718..903
                     /locus_tag="ACG8ER_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5051501.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1061..1228
                     /locus_tag="ACG8ER_003017"
     CDS             1061..1228
                     /locus_tag="ACG8ER_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051502.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1846..2513
                     /gene="vanR-B"
                     /locus_tag="ACG8ER_003018"
                     /pseudo
     CDS             1846..2513
                     /gene="vanR-B"
                     /locus_tag="ACG8ER_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
     gene            2516..3859
                     /gene="vanS-B"
                     /locus_tag="ACG8ER_003019"
     CDS             2516..3859
                     /gene="vanS-B"
                     /locus_tag="ACG8ER_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS5051503.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            4030..4836
                     /locus_tag="ACG8ER_003020"
     CDS             4030..4836
                     /locus_tag="ACG8ER_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFS5051504.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4854..5681
                     /gene="vanW-B"
                     /locus_tag="ACG8ER_003021"
     CDS             4854..5681
                     /gene="vanW-B"
                     /locus_tag="ACG8ER_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS5051505.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            5678..6649
                     /locus_tag="ACG8ER_003022"
     CDS             5678..6649
                     /locus_tag="ACG8ER_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS5051506.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            6642..7670
                     /gene="vanB"
                     /locus_tag="ACG8ER_003023"
     CDS             6642..7670
                     /gene="vanB"
                     /locus_tag="ACG8ER_003023"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS5051507.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            7676..8284
                     /locus_tag="ACG8ER_003024"
     CDS             7676..8284
                     /locus_tag="ACG8ER_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS5051508.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         2412 a         1775 c         2196 g         2113 t
ORIGIN      
        1 cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt
       61 gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat
      121 agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa
      181 aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat
      241 tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt
      301 cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa
      361 gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg
      421 aatggccccg tcgttttcga tgttggcaag atagcgccag tcaatattta ttttctctgc
      481 cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg cgccgaaagt
      541 ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta cattgtatat
      601 ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta tatttcacat
      661 tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag gtgtactatg
      721 gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg atggataaac
      781 tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat ctggctcatt
      841 cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc ggttactgaa
      901 tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa gcatggtatt
      961 ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga taaaaagtat
     1021 tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc gaaactttat
     1081 catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg tatcagccgt
     1141 atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct ccagacattg
     1201 gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat accggggagg
     1261 tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac atttcctgta
     1321 actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt ttctactgtc
     1381 ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac cggagggagt
     1441 acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca aaaaagaggc
     1501 cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg ccgcaggcgg
     1561 gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg ccagtattat
     1621 cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag gtgttaatta
     1681 ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc atgaaaagaa
     1741 gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga aactacaggg
     1801 aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc gatacgaatt
     1861 ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct ggctgaggca
     1921 ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt ttacgaaaac
     1981 acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca tgaacttttg
     2041 cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct gtcggatgac
     2101 gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa gccattcaag
     2161 atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc gctggcaaag
     2221 gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact ttgtgacggt
     2281 acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt gcagaacaaa
     2341 ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga ctttgagggt
     2401 gatggcagca cagtccacac tcccacactc atatcaaaaa tttgcgggcc aagctgccgg
     2461 aaaatatcat caaaaccatt cgcggtgtag gataccgatt ggaggaatca taataatgga
     2521 aagaaaaggg attttcatta aggttttttc ctatacgatc attgtcctgc tactgcttgt
     2581 cggtgtaaca gcaacactgt ttgcacagca atttgtgtct tatttcagag tgatggaatt
     2641 acagcaaaca gtaaaatcct atcagccatt ggtggaactg attcagaata gcgataggct
     2701 tgatattcaa gaggtggcag ggctgtttca ctacaataac caatcctttg agttttatat
     2761 tgaagataaa gagggaagcg tactctatgc cacaccaaat gccaatacat caaatagttt
     2821 tagacccgac tttctttatg tggtacatag agatgataat atttcgattg ttgctcaaag
     2881 caaggcaggt gtgggattgc tttatcaagg gctgacaatt cggggaattg ttatgattgc
     2941 gataatggtt gtattcagcc ttttatgcgc gtatatcttt gcgcggcaaa tgacaacgcc
     3001 gatcaaagcc ttagcggaca gtgcgaataa aatggcaaac ctgaaagatg taccgccgcc
     3061 gctggagcga aaggatgagc ttggcgcact ggctcatgat atgcattcca tgtatgtcag
     3121 gctgaaagaa accatcgcaa ggctggagga tgaaatcgca agggaacatg agttggagga
     3181 aacacagcga tatttctttg cggcagcttc tcatgagcta aaaacgccca tcgcggctac
     3241 aagcgttctg ttggagggaa tgcttgaaaa tatcggtgac tacaaagatc attctaagta
     3301 tctgcgcgaa tgcatcaaaa tgatggatag gcagggcaaa atcatttccg aaatactgga
     3361 gcttgtcagc ctgaatgatg ggagaatcgt acccatagct gaaccgttgg acatagggcg
     3421 cacggttgcc gagttgctgc ccgattttca aaccttggca gaggcaaaca accagcggtt
     3481 cgtcacagat attccagccg ggcaaattgt cctgtccgat ccgaggctgc tccaaaaggc
     3541 actatccaat gtcatattga atgcagttca gaacacgccg cagggaggcg aggtacggat
     3601 atggagtgag cctggtgctg aaaaatgccg cctttttgtt ttgaacatgg gcgttcacat
     3661 tgatgatact gcgcttccaa ggctgttcac cccattctat cgcattgatc aggcgcgaag
     3721 cagaaaaagt gggcgaagcg gtttaggact tgccatcgta caaaaaacgc tggatgccat
     3781 gagcctccag tatgcgctgg aaaacacctc ggatggcgtt ttgttctggc tggatttacc
     3841 gctcacatca acattgtaaa tatttaaaac ttaaatgatt ttgaccgaca ggtataaccc
     3901 tgccggtctt tttttgctgc tacaggaaaa ctacagactg gctacaggga aagtacagat
     3961 gcgcttgcca taataacaat cgtaccagcc acaaatcgta gttttattgc aaaggaggca
     4021 ttcgatcaaa tggaaaaaag caactatcat tccaatgcgg atcatcacaa acggcatatg
     4081 aaacaatccg tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc
     4141 acgctgtttt tggggtggag actggcttcc gtattggagg caacacagat accgcccatc
     4201 cctgcaactc atacaggcag cagcactgac gtagtggaga atttggagga aaacgctctt
     4261 gccaccgcca aagaacaggg agatgaacag gaatggagcc tgattttagt gaacaggcag
     4321 aaccccatcc ccgcacagta cgatgtggag cttgagcaac tatcaaatgg tgagcggata
     4381 gatattcgga tttctcccta tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt
     4441 tacccgattg tcgcatccgg ataccgaaca acagaaaaac agcaagaaat tatggatgaa
     4501 aaaattgccg aatataaggc gaaaggctac acctctgcac aggctaaagc ggaagcagaa
     4561 acttgggtgg ccgtgccggg aacgagcgag catcagcttg gtcttgctgt ggatatcaat
     4621 gcggacggaa ttcattcaac aggcaacgag gtttatagat ggctggatga aaacagctat
     4681 cgttttggtt ttattcgtcg ctacccacca gacaagacag agataaccgg tgtgagcaac
     4741 gagccgtggc attaccgata tgttggcatc gaagctgcca cagagatgta caaccaaggg
     4801 gtttgccttg aggaatattt aaaaccagaa aaatgagaaa aggatgttat gttatggaca
     4861 gaaaaagatt gacacagcgt ttcccgttcc tgcttccaat gagacgggcg cagagaaaaa
     4921 tgtgctttta tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg ataggggaaa
     4981 aatcgctttc ccatttgctc tttgaaacgg attgcgcatt atacaaccac aataccggat
     5041 ttgacatgat ataccaggaa aacaaggtgt tcaacttaaa gctggcagca aagaccttaa
     5101 acggcttatt gataagaccg ggggaaacct tttctttctg gtggctggta cgccatgcgg
     5161 acaaagatac cccctataaa gacggcctta cggtgaccaa tggtaaactc accaccatgt
     5221 cgggcggcgg tatgtgccag atgagcaatt tactattttg gatgttcctg catacgccat
     5281 tgacaattat ccagcgcaga ggtcacgaag taaaggagtt tccagagcca aacagtgacg
     5341 agattaaagg ggtggatgca accatttcag agggctggat tgatttaaaa gtgcgaaacg
     5401 ataccgactg cacctaccaa atatgggtga ccttagatga tgagaaaatc atcggtcagg
     5461 tgtccgccga caaagagccc caagcattat acaaaattac aaatggcagt atccagtatg
     5521 tccgtgaaag tggcgggatt tatgaatatg cccaggttaa acggatgcaa gttgccttag
     5581 gtaccgggga aataatagat tgcaagctgc tttatacaaa caaatgcaaa atctgttatc
     5641 ccctcccgga aagtgtggat attcaggagg agaaccaatg agaaaaagtg tgggcattac
     5701 tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc cggattttca
     5761 tattatccct acgctgatta gcgatgcgat atcggcagac aacgcaaaat tggccgctgg
     5821 caatcaatgc gttagcgtag gccataagtc cgaggtttcc gaggcgacaa ttcttgcgct
     5881 gagaaaggtc ggggtaaaat acatttctac ccgcagcatc ggctgcgatc acattgatac
     5941 gactgccgcc gagagaatgg gaatctcggt tggcacggtt gcgtattcgc cggacagcgt
     6001 tgcggattat gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca
     6061 cgccgtggcg caacaagatt tcagattgga tcgtatccgg gggaaagaac tgggggatat
     6121 gactgtggga gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg
     6181 atttggatgc catgtgctgg cctatgataa cagccgaaaa atggatgcag attatgtcca
     6241 gcttgatgag cttctaaaaa acagcgatat tgttacgctc catgtgccgc tttgtgcgga
     6301 tacccgccat ctgatcggtc agaagcaaat tggagagatg aagcaaggcg catttttaat
     6361 caacaccggg cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg
     6421 aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg tttataccga
     6481 ttgctcgcag aaagtgcttg accatccctt tttgtcgcag cttttaagga tgccgaatgt
     6541 gatcatcaca ccccatacgg cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa
     6601 aacaatcagg aattgtctca attttgaaag gagtttacag catgaataga ataaaagtcg
     6661 caatcatctt cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc gcaatagaaa
     6721 ttgctgcgaa cattgatacg gaaaaattcg atccgcacta catcggaatt acaaaaaacg
     6781 gtgtatggaa gctatgcaag aagccatgta cggaatggga agccgacagt ctccccgcca
     6841 tactctcccc ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa
     6901 cacggcgtat tgatgtggct ttcccggttt tgcatggcaa atgcggggag gatggtgcga
     6961 tacaggggct gtttgtattg tctggtatcc cctatgtggg ctgtgatatt caaagctccg
     7021 cagcttgcat ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc
     7081 ccgaatttca aatgattgat aaaggtgaca agccggaggc gggtgcgctt acctaccctg
     7141 tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt aaccaaagta aacggtacgg
     7201 aagaacttaa cgctgcgata gaagcggcag gacaatatga tggaaaaatc ttaattgagc
     7261 aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat gatttgattg
     7321 tcggcgaagt ggatcaaatc cggctgagcc acggtatctt ccgcatccat caggaaaacg
     7381 agccggaaaa aggctcagaa aatgcgatga ttacagttcc cgcagacatt ccggtcgagg
     7441 aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga tgcagagggc
     7501 ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat cgttctaaat gaggtcaata
     7561 ccctgcccgg ttttacatcg tacagccgct acccacgtat gatggccgcc gcaggaatca
     7621 cgcttcctgc actgattgac agcctgatta cattggcgtt aaagaggtga taagtatgga
     7681 aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc
     7741 cacatgggat aacttcacag gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg
     7801 cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg cgcttggcta
     7861 tggcttgctt ttatgggatg gatatcggcc aagaactgcg gtggactgct tcctgcgttg
     7921 ggcagcgcaa ccggaggaca atctcacaaa agaaaaattt taccccaata tagagcgagc
     7981 cgagttgatt acaaagggtt atgtggcctc acaatccagc catagccgtg gaagcgcaat
     8041 tgatcttacg ctctaccacc tggatacagg ggaacttgtt tcaatgggaa gtaacttcga
     8101 ttttatggac gaacggtcgc atcatacagc aaaagggata ggggatgcag aggcacaaaa
     8161 tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc gctttgaatg
     8221 gtggcactat aagttgattg atgagccata ccccgatacc tattttaatt ttgccgtttc
     8281 ataatgaaag tattggtttt gtatcagatt ttggatttga taaaatctat gctggtcatg
     8341 gacaatcttt tactggaaag tggttaaagc atcttttgta atacattgca aattatagta
     8401 ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc
     8461 cattttgtat tttgctggcg gcggttttga gaaatg
//
LOCUS       DAZUMO010000239         6676 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.111, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000239 DAZUMO010000000
VERSION     DAZUMO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.111"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..782
                     /locus_tag="ACG8ER_003025"
                     /pseudo
     CDS             117..782
                     /locus_tag="ACG8ER_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            818..1063
                     /locus_tag="ACG8ER_003026"
     CDS             818..1063
                     /locus_tag="ACG8ER_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5051509.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1166..2035
                     /locus_tag="ACG8ER_003027"
     CDS             1166..2035
                     /locus_tag="ACG8ER_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5051510.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLLECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2016..2750
                     /locus_tag="ACG8ER_003028"
     CDS             2016..2750
                     /locus_tag="ACG8ER_003028"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5051511.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2783..3691
                     /locus_tag="ACG8ER_003029"
     CDS             2783..3691
                     /locus_tag="ACG8ER_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5051512.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3688..4230
                     /gene="sat4"
                     /locus_tag="ACG8ER_003030"
     CDS             3688..4230
                     /gene="sat4"
                     /locus_tag="ACG8ER_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS5051513.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4323..5117
                     /locus_tag="ACG8ER_003031"
                     /note="aph(3')-IIIa"
     CDS             4323..5117
                     /locus_tag="ACG8ER_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5051514.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            5393..5524
                     /locus_tag="ACG8ER_003032"
                     /pseudo
     CDS             5393..5524
                     /locus_tag="ACG8ER_003032"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            5600..6345
                     /locus_tag="ACG8ER_003033"
                     /pseudo
     CDS             5600..6345
                     /locus_tag="ACG8ER_003033"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            6456..6635
                     /locus_tag="ACG8ER_003034"
                     /pseudo
     CDS             6456..6635
                     /locus_tag="ACG8ER_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
BASE COUNT         2287 a         1005 c         1461 g         1923 t
ORIGIN      
        1 tttccattta gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat
       61 aaagcacact ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt
      121 atcaaaatta taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag
      181 aaaatgattt tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc
      241 ttccttatta tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta
      301 ttctttgtgc ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag
      361 atagtattcg gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc
      421 gttttcgcgt tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg
      481 agctactggt tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa
      541 ttgaagcgga tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg
      601 atagccttga tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac
      661 ctgagttaat agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc
      721 aatctttgga agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag
      781 aagggggctg acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt
      841 ggaagaagct attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga
      901 tttattcaag ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac
      961 gttattttta aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc
     1021 atctaatcag cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt
     1081 aaaaaaatta taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag
     1141 tgaatcgata aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa
     1201 attatcctct ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac
     1261 ccatacagag gattcggata tagatatcgg catctattac aattcagaat catttgacat
     1321 aaatactatt aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt
     1381 acctcccgga gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta
     1441 tcatgtggat ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga
     1501 gcacggaatt gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat
     1561 gtatcgagga gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga
     1621 attaaaaaaa caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt
     1681 tatgtttgaa gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa
     1741 atattatatt gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc
     1801 atgtaataat gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga
     1861 acataaacct gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc
     1921 acttttggaa tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc
     1981 ggagataaat aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata
     2041 atatattttt tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg
     2101 gagaatggga gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt
     2161 taggatgcgg ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag
     2221 taggtgttga tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac
     2281 agattgaata tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg
     2341 taatactaag ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga
     2401 tatataggat gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta
     2461 ctgctcatgg aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg
     2521 tggacaatta ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa
     2581 aatatcatag aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa
     2641 atcagattgt ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg
     2701 aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa
     2761 aaaataaacg aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc
     2821 tttagcagaa caggatgaac gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat
     2881 taatatacct aaagatgaat ttcaggatta tgatattaca tattttgtaa gtgatataga
     2941 accgtttata tctaatgatg actggcttaa tcaatttggg aatataataa tgatgcaaaa
     3001 gccggaggat atggaattat tcccacctga agaaaaggga ttttcctatc ttatgctatt
     3061 tgatgattac aataaaattg atcttacctt attgcccttg gaagagttag ataattacct
     3121 aaagggcgat aaattaataa aggttctaat tgataaagat tgtagaatta aaagggacat
     3181 agttccgact gatatagatt atcatgtaag aaagccaagc gcaagggagt atgatgattg
     3241 ctgcaatgaa ttttggaatg taacacctta tgttattaaa ggattgtgcc gtaaggaaat
     3301 tttatttgct attgatcatt ttaatcagat tgttcgccat gagctgctga gaatgatatc
     3361 atggaaggtc ggcatcgaaa caggctttaa attaagtgta ggcaagaact ataagtttat
     3421 tgaaaggtat atatccgagg atttgtggga gaaacttttg tccacctacc ggatggattc
     3481 ctatgaaaac atatgggaag cattatttct atgccatcaa ttgttcaggg cggtatccgg
     3541 tgaggtggcg gaaaggcttc attatgccta tccggagtat gataggaata taacaaaata
     3601 taccagggac atgtataaaa aatacactgg taaaaccggc tgcctggata gcacatatgc
     3661 cgctgatata gaagagaggc gggaacagtg attacagaaa tgaaagcagg gcacctgaaa
     3721 gatatcgata aacccagcga accatttgag gtgataggta agattatacc gaggtatgaa
     3781 aacgagaatt ggacctttac agaattactc tatgaagcgc catatttaaa aagctaccaa
     3841 gacgaagagg atgaagagga tgaggaggca gattgccttg aatatattga caatactgat
     3901 aagataatat atctttacta ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa
     3961 aattggaacc ggtacgctta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     4021 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     4081 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     4141 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     4201 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     4261 ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat
     4321 aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg
     4381 taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa
     4441 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg
     4501 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg
     4561 aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg
     4621 aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca
     4681 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct
     4741 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg
     4801 aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc
     4861 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag
     4921 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg
     4981 acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc
     5041 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac
     5101 tggatgaatt gttttagtac ctagatttag atgtctaaaa agctttaact acaagctttt
     5161 tagacatcta atcttttctg aagtacatcc gcaactgtcc atactctgat gttttatatc
     5221 ttttctaaaa gttcgctaga tagagttcta tattagagat gtaaagagtt ttggtgaaga
     5281 ggtttgtgag tatggctttt ataaccaggg tgcaatgaga agcactaaca gcagctagct
     5341 taagaacgca taggatatac tttttatgga agtcacagag gaggaataaa atttgaacag
     5401 gattaacaga gtaacctcta ttcttattca gctgcaatca aaaaaaataa ttcctgccaa
     5461 agaaattgca cagcgattta atataagctt aaggacagtt tacagagata tccggacact
     5521 tgaagaagcc ggaataccaa ttggatctga ggccggaaaa ggatattttc ttgtagaggg
     5581 cttcctactt ccgccggtaa tgtttacagc ggcggaagtg ggtgcattga ttacagcagg
     5641 gaaattttta aattgccatg gagatgaatc atttataaag gattttgatt cagctatgta
     5701 taaaatcaaa tctattttga agcatggtga aaaaaactat gcacaggagt tggagaacag
     5761 tattaacgtg tacagcacat ctggacagaa aaatacattg gcggataacg ttattgcagc
     5821 aattcagact gcaatctgca ataaaagggt aatatcaatt caatatcctg catcaggagg
     5881 gcaagaaccg gaaagcagaa tgatagagcc tatatcactg gggttttatg aacagaactg
     5941 gtacttaatc ggttttgcag gctaagaaat gaatatagga attttcgtgt ggatcggatt
     6001 aaaagcattt gcatagaaga agagctctgt caaagccatg atggctcttt ggaacaaata
     6061 ttaaaacaaa tgctttcata taaaaaacta tataatgtaa ttcttagagc tgaaaaaggg
     6121 gagacataca attctatcaa gaacaggtat tctctcggct tccttgaaga aaccgatttg
     6181 ggaagtaaaa tggaaatcga attccagaca gattcctttg agatattgag caagcagctg
     6241 attgaatatg gatcaggcat tgaaatagtc caacctgatg aattaaaatg cataacccgt
     6301 aaacacctgg cacaaattac gaatcactgc ctaaacttga tttagagaaa gcgattggtc
     6361 tgttatgtat tttttgtaag gtttcatatc ccctgctgac atacagctgt cagtgtatta
     6421 tgatattcta ctatcaaaaa ggaggattga aaaatatgaa accaaagata attatgcaca
     6481 cacaaattag ccttgatggc cgcattaaag gctttgataa tccggaagtt tactaccagg
     6541 ttgccggggg aattcattcg gatgcggtgc tgtttgggtc caatactgtt tttactgcat
     6601 ttgaaaaata tcctgctgaa acagaggctg attttggata aagtccgtat aattgtgtaa
     6661 aagtaaaaag gccata
//
LOCUS       DAZUMO010000240         6516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.116, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000240 DAZUMO010000000
VERSION     DAZUMO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.116"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..1104
                     /locus_tag="ACG8ER_003035"
     CDS             193..1104
                     /locus_tag="ACG8ER_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFS5051515.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1257..1742
                     /gene="vanZ-A"
                     /locus_tag="ACG8ER_003036"
     CDS             1257..1742
                     /gene="vanZ-A"
                     /locus_tag="ACG8ER_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS5051516.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2267..3907)
                     /gene="merA"
                     /locus_tag="ACG8ER_003037"
     CDS             complement(2267..3907)
                     /gene="merA"
                     /locus_tag="ACG8ER_003037"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFS5051517.1"
                     /translation="MNQFRMRIQGMTCTGCEKHIEKALESMGALNAKADFSQSEAVFQ
                     LPDDYAIENAIEAVKKINYQPEEVEKLPVQKRVMTNNKEDYDLLIIGSGGAAFSAAIK
                     AVEYGSKVGMIERGTVGGTCVNIGCIPSKTLLRAGEINQSAHVNIFEGLHTSTGEVEL
                     DRLVDQKNELVDDLRKQKYTNLIDDYGFDLIKGEAKFIDENTVTVSGQTYSAQRFLIA
                     TGASPLLPRITGLKEVDYLTSTTLLELRKVPKRLTIIGSGYIGRELGQLFHNLGSEVT
                     LIQRSKRLLKEYDPEISEAMEKALIEQGIKLISGATYERVEQDGNIKKVHIIVNGKNK
                     VIESDELLVGTGRKPNTEVLNLSAAGVQVGPNNEIKINAFAQTSNRKIYAAGDVTLGP
                     QFVYVAAYEGGIAAGNAIGGLNKAIDLSVVPGVSFTSPQFATVGLTQQQAKEKGYEVK
                     KAVLSLENVPRAIVNHNTTGVFKLVVDRKTQKILGVHIVSENAGEVIYGASLAVKFGL
                     TVADLKDTLAPYLTMSEGLKLAALAFDKDISKLSCCAG"
     gene            complement(3921..4319)
                     /gene="merR"
                     /locus_tag="ACG8ER_003038"
     CDS             complement(3921..4319)
                     /gene="merR"
                     /locus_tag="ACG8ER_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFS5051518.1"
                     /translation="MAYFISSFSEKCGVNKESIRYYERKNLLPEPDRTNSGYRVYSEE
                     DVKRVKFIKHLQEIGFSLNEIYKLLGVVDKDNIRCEGMFEFVSKKEREIQKKIENLNR
                     IEYMLKDLRARCPDEKELYACPIIEVLIEN"
     gene            4636..5187
                     /locus_tag="ACG8ER_003039"
     CDS             4636..5187
                     /locus_tag="ACG8ER_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5051519.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            5500..6042
                     /locus_tag="ACG8ER_003040"
     CDS             5500..6042
                     /locus_tag="ACG8ER_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFS5051520.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         2072 a         1158 c         1154 g         2132 t
ORIGIN      
        1 acgtgagttg aaattttgac aacctaattt ttctaaggaa attggtgata atagtaaatc
       61 cagtagggcg aaataattga ctgtaattta cggggcaaaa cggcacaatc tcaaacgaga
      121 ttgtgccgtt taaggggaag attctagaaa tatttcatac ttccaactat atagttaagg
      181 aggagactga aaatgaagaa gttgtttttt ttattgttat tgttattctt aatatactta
      241 ggttatgact acgttaatga agcactgttt tctcaggaaa aagtcgaatt tcaaaattat
      301 gatcaaaatc ccaaagaaca tttagaaaat agtgggactt ctgaaaatac ccaagagaaa
      361 acaattacag aagaacaggt ttatcaagga aatctgctat taatcaatag taaatatcct
      421 gttcgccaag aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga cgaattaata
      481 aatggatacg ggttgcttga tagtaatatt tatatgtcaa aagaaatagc acaaaaattt
      541 tcagagatgg tcaatgatgc tgtaaagggt ggcgttagtc attttattat taatagtggc
      601 tatcgagact ttgatgagca aagtgtgctt taccaagaaa tgggggctga gtatgcctta
      661 ccagcaggtt atagtgagca taattcaggt ttatcactag atgtaggatc aagcttgacg
      721 aaaatggaac gagcccctga aggaaagtgg atagaagaaa atgcttggaa atacgggttc
      781 attttacgtt atccagagga caaaacagag ttaacaggaa ttcaatatga accatggcat
      841 attcgctatg ttggtttacc acatagtgcg attatgaaag aaaagaattt cgttctcgag
      901 gaatatatgg attacctaaa agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa
      961 tatgagatct tttattatcc tgttactaaa aataccacca ttcatgtgcc gactaatctt
     1021 cgttatgaga tatcaggaaa caatatagac ggtgtaattg tgacagtgtt tcccggatca
     1081 acacatacta attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag
     1141 cattattgta ctagcacttt tggggtaacg ttagcttttt aatttaaaac ccacgttaac
     1201 taggacattg ctatactaat gatacaactt aaacaaaaga attagaggaa attatattgg
     1261 gaaaaatatt atctagagga ttgctagctt tatatttagt gacactaatc tggttagtgt
     1321 tattcaaatt acaatacaat attttatcag tatttaatta tcatcaaaga agtcttaact
     1381 tgactccatt tactgctact gggaatttca gagagatgat agataatgtt ataatcttta
     1441 ttccatttgg cttgcttttg aatgtcaatt ttaaagaaat cggattttta cctaagtttg
     1501 cttttgtact ggttttaagt cttacttttg aaataattca atttatcttc gctattggag
     1561 cgacagacat aacagatgta attacaaata ctgttggagg ctttcttgga ctgaaattat
     1621 atggtttaag caataagcat atgaatcaaa aaaaattaga cagagttatt atttttgtag
     1681 gtatactttt gctcgtatta ttgctcgttt accgtaccca tttaagaata aattacgtgt
     1741 aagatgtcta aatcaagcaa tctgatcttt catacacata aagatattga atgaattgga
     1801 ttagatggaa aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg
     1861 gtgataaagt aaaaagctta cctaacacta tagtaacaaa gaaagcccaa ttatcaattt
     1921 tagtgctgag gaattggtct ctttaataaa tttccttaac gttgtaaatc cgcattttcc
     1981 tgacggtacc cctttaagcg gtagaatcct tttttgatac gatttaatta tctatattaa
     2041 gcacgtaagg caacaatcgc atcttcaata atatcttgct gcgttgccta aatgagcaat
     2101 tgcgtttcta aatagtcttt tatttcttca acctccacta gaatagagct attaattggc
     2161 acgtagacgt ttgctgatgt cataatcttc aactcctatc gttatagata ataacgaaag
     2221 ataaaccaag gtgttaccag ctactacaag aaactaaata gaaatattaa cctgcacaac
     2281 aagataattt cgaaatatct ttatcaaagg ctagagctgc taactttaag ccttctgaca
     2341 ttgttagata aggcgctaaa gtatctttta aatcagctac ggtcaatcca aatttaacgg
     2401 ctaaggatgc tccataaata acttctcctg cattttcaga aacgatatga actcccaaaa
     2461 tcttctgtgt ttttctatca accacaagtt taaacacccc tgtcgtatta tgattgacaa
     2521 tcgctctagg aacattttct aatgacaata ccgctttttt tacttcgtat cctttttctt
     2581 tcgcttgttg ttgtgttaac ccaacagttg caaattgcgg actggtaaat gatactccag
     2641 gaactaccga taaatcgatt gctttattta acccaccaat tgcatttcct gctgcaattc
     2701 ctccttcata ggctgctaca tagacaaact gaggtcctaa tgtcacgtct cctgctgcat
     2761 aaatttttct attacttgtc tgagcaaaag catttatctt gatttcattg tttggcccaa
     2821 cttgaacgcc tgctgcgctc aagttcaata cttctgtatt cggttttctt cctgttccaa
     2881 ctagtagctc gtctgactca atgaccttat ttttgccgtt tacaataata tgaacttttt
     2941 taatatttcc gtcttgctct actcgttcat aagttgctcc ggaaatgagc tttattcctt
     3001 gttcgattaa agctttctcc attgcttcag aaatctctgg atcatactct tttaaaagtc
     3061 gtttgcttct ttgaataagg gtaacctcag aacccagatt gtggaagagc tgtcccagtt
     3121 ctcttccaat ataacccgat ccaataattg ttaatcgttt tggtactttt cttagttcaa
     3181 ggagggttgt gctagttaaa taatctactt ctttaagtcc agtaatacga ggtaagagtg
     3241 gagaagcacc cgttgcgatt aaaaaccgtt gggcagaata tgtttgccca ctaactgtca
     3301 cagtgttttc gtcaatgaat tttgcttctc ctttaattaa atcaaaacca tagtcgtcaa
     3361 ttaaattagt gtatttttgt ttgcgtaaat catcaactag ttcatttttt tgatcgacta
     3421 agcggtctaa ttctacttct cccgtagaag tgtgcaatcc ttcaaaaata tttacatgtg
     3481 ctgattgatt gatttctcca gctcgaagca gcgtttttga agggatacaa ccaatgttca
     3541 cacaggttcc gcctacagtt ccgcgttcaa tcatgcccac ctttgatcca tactcgactg
     3601 cttttatagc tgctgaaaaa gctgctccac cagaaccgat aatgagcaaa tcataatctt
     3661 ccttgttatt agtcatcacg cgtttttgaa caggtaactt ttctacttct tcgggctgat
     3721 aatttatttt tttaactgct tctatcgcgt tttctattgc atagtcgtct ggtaattgaa
     3781 ataccgcttc gctttgactg aaatctgctt tagcgttgag agctcccatg ctctcaagcg
     3841 ctttttctat atgtttttca cagccagtac aggtcattcc ttgaatgcgc attctaaatt
     3901 gattcatttt cctacccccg ttaattttct attaatactt ctatgatagg acacgcgtat
     3961 agttcttttt catctggaca tcttgctcgt aaatctttta gcatatattc tattctattc
     4021 aaattctcga tttttttttg aatttctctt tctttcttag agacaaattc aaacatacct
     4081 tcacaacgaa tgttgtcttt atctacaaca ccaagtaatt tataaatttc atttaaagaa
     4141 aagccaattt cttgtaggtg tttaatgaat tttactcgtt ttacatcttc ctctgaataa
     4201 actcgataac cggaattagt ccgatcgggt tctggtagta agttttttcg ttcgtaatat
     4261 cgaatggatt ccttatttac accacatttt tcagagaagg agctaataaa ataagccatg
     4321 ctatcacatc ctttttttaa ttataaacca tgtacttagg tacatgtcca accaaaaata
     4381 atacgaaggg gggaatatca ttttccttct tatttacttt caaaaatgaa gtgaaaatta
     4441 aatgtggtac caataaattt tgagacttct tttataataa ataatatcgg acctaaatac
     4501 atgaaattac ataaaatcat gtatcaagaa tcctatcctt atagggttct ttttttttac
     4561 tccccctaaa cacacgattt cgtgttgtaa taactataat catgtattcg aacagactaa
     4621 aaaggaggga ttgtcatggg ctataatgtt cagccactac ggtcgcaaca ggagattaat
     4681 gattttttat tttgcctgcg acggaataaa aatgcggaac gtgacgtttt cttgtttcta
     4741 attggaatta acagcggtct gcgcatgtcc gacatcgtta aactcaagaa aaaagacgtg
     4801 attacctcaa aaaacccgcg catcgtggag caaaagacag gaaaaacacg cattttatac
     4861 ttaagcagct tacaggactt gattcacggc tataccgcac ccttagatcc ggaggcttac
     4921 ttgtttccca gtactaaggg cggccattta gaagtcaata cggtctacca gatgtttcaa
     4981 aaggtcgcga agctgttagg aagggacgat atcggcaccc acaccctacg aaaaaccttt
     5041 ggttaccact attacaagaa aaccaaagac gtggccacct taatggaaat ctttggtcac
     5101 agcagcgaga agattaccaa acgctacatc ggaattaatg aagatgaaat cagtgaaacg
     5161 ttattaaatt ttcggttagg tttttgattg tattatataa tagataagca cagtaagacg
     5221 gttataatca cccacagtat tggacaaatc aacctttttt tggtatatta tatatacaaa
     5281 tgagagcagg ctcatagtca acttgatata aatccggaac ataagggtcc ggcgggtggc
     5341 gagcaccaaa actaaactcg atacaaatcc ggaacgtaag ggtccggcgg gtggcaagca
     5401 ccaaaactaa acctgtacaa aaggctgctc ttttgagcag ctttttttat tgggaaaaag
     5461 gagcattctt cgcacgcggc aaaaaaagaa tacaaacgca tgtctataaa agagacaacc
     5521 caaattacca gacagttaaa cgcgatttat aaggctgctc atctcttaca agaacatttt
     5581 gtagataaaa aagtatcctt tgtcggagaa gtctcaacgg tagcgattat tttttcgaca
     5641 actaatttta tgcacctctg tggcattgat tatcgcagag gcacacactt gttttttcaa
     5701 gatgccttag acaggaaaat aaatttacag gatattcaaa tcaaaacgga tgggacgacc
     5761 tttcagaaat tacaagtaat aggtagcctt gatttattat tggggaagca tatttctatt
     5821 gttggcagag gtgtttactc ttctcttcgc tatgatgcag ctattcgaac aaggaaaaaa
     5881 atattggctc tgtctttgaa acaaaacgga ttaatctata tccctatctc tttgttaaac
     5941 ctatcatcaa aggaaattgg acctggacaa aaagttactg gcatttttag cgaggattta
     6001 acatccgggg aacttaaaat gattatggaa gtcatcgatt aatcattaat agtgatatgc
     6061 gacgtcattt tgaagtaatt attctatttt gggttataaa ataaagcatt ttaagacgat
     6121 ttcaaatcat tcatgtgggt tatttatcac ggatgagctt gaaatcgtct tttatacgta
     6181 tcaaattgaa atgttcggca tacaatctat cgttgttact acattactaa ttgtcacttg
     6241 ttaaggaact tggatccttt ctaaacattc cctagagata aattttactt tatccctccg
     6301 ttaatatcca atttaagcac ctttccgatt cagggttaat ttgcatatca agtgcagctt
     6361 gtgttctagt aagattagcc cttttagtcc ggctgagttg acttatggat ataagatatg
     6421 gtcatagcaa atagatacta cgtatagtct gttaatttag tgtcattaat cttaagttgg
     6481 ataggctaca attaactaga cagaaaaatt aaggtg
//
LOCUS       DAZUMO010000241         4185 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000241 DAZUMO010000000
VERSION     DAZUMO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.141"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..1613
                     /gene="msr(C)"
                     /locus_tag="ACG8ER_003041"
     CDS             135..1613
                     /gene="msr(C)"
                     /locus_tag="ACG8ER_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5051521.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1660..2106
                     /locus_tag="ACG8ER_003042"
     CDS             1660..2106
                     /locus_tag="ACG8ER_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5051522.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2305..2820)
                     /locus_tag="ACG8ER_003043"
     CDS             complement(2305..2820)
                     /locus_tag="ACG8ER_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5051523.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2937..4139
                     /locus_tag="ACG8ER_003044"
     CDS             2937..4139
                     /locus_tag="ACG8ER_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5051524.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1243 a          707 c          911 g         1324 t
ORIGIN      
        1 gatttggtga tttagtcata gttatttcgt tcagagtagg aaaagatgtg atgcagtttt
       61 tttgcaaaca gatttttctg ctctttttta gtatcgataa ttgttgtttt acatgaaata
      121 ggagtgtaaa acatatggaa aatttagcag taaatataac aaatctacaa gtcagctttg
      181 gcaaccagct agaactatct attgattctc ttcgtgtcta tcagcaagat cgtataggga
      241 tcataggaga aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt
      301 ttcccgatca tgggaaaatc caaacagaga tcaccttcaa ctacctgcct caattaacct
      361 atctcgctga agcaaaggac ctaaatttgg aattagccag tcatttccag ttaagactgg
      421 aagaaacttc agagcggaaa tggagcggag gggaagaacg aaagatcgag ttgatacgtc
      481 ttctttcttc ttatgaacaa gggatgcttc tagacgagcc aacaacccat ctagatagaa
      541 aaagtattga tcgactgatt gaagagcttc gttattatta tggtacgctg gtttttgtta
      601 gtcatgatcg ctattttcta gatgagctag catcgaaaat ctgggaagta aaagacggag
      661 aaatccgaga gttttcgggg aattatagtg cctatctcac tcaaaaggaa ttggagaaaa
      721 agactcagct acgagaagca gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga
      781 tccaagaaaa gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc
      841 aacaaatcag actggatcgg ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca
      901 tccaaaagaa tgcgaaaaca ttagagagaa gactccaaaa aataggagaa acaaccaagc
      961 cgcagcagat gaaacaaatc cgttttccag taccaaaatc tcttgaactc cacagccgct
     1021 atcccatcat gggacaaaat gtccaattgg aaagaagtgg gagaacatta ctggtaaatg
     1081 gtgattttca gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta
     1141 agacaacctt attagaacat atccgcaaac aaggagaagg aatccttctc tctccgaaag
     1201 taagctttca agtatatcag caaaagggtt atcaaatgac atctgaagaa tccatcattc
     1261 gttttgtcat gagacaaaca gagttttcag aatcgcttgt ccgtagtttg ctgaatcact
     1321 tagggtttgc tcaggaaact ttgacgaaac cgttatgtac attaagtggg ggagaagcga
     1381 cccgtctgac gattgctttg ctttttacta agccaagtaa tgtgttgctg ttagatgaac
     1441 cgactaattt tattgatatg gcaacgatcg aagctttaga gaagctaatg caaatatatc
     1501 cgggaacgat tttgtttact tctcatgatt cttactttgt cgagcgtacg gctgatgaag
     1561 tttatgaaat aaaagggcag aaaataaaaa aagtacttac gagaaatttt taaatgtgct
     1621 acgatgattt tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa taaaaaagaa
     1681 ggaataattt ttacaacaat gatgtgtttt ttgatggtct tggggatgag cgcgtataat
     1741 ttatggctgc atcaagatct tcatttatca agtttgttga ttggtctagt gcctggattc
     1801 gtggtggctt tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt
     1861 aaactaccag taaatccaga aagcaaggtc caaatgatct taactatttc atgcttgatg
     1921 gttttaggaa tggtgacatg tatgtcttta ttcggtgtga tcatggaaca aggaatacct
     1981 gatcaattat tctctgctta tatgaatgct tggaaaatga atgtgattgc cgctttaccg
     2041 ttgcagttga tcatcgttgg cccgctttca agaagtgtgc taaaagcaat tcaaagtaat
     2101 gaatgatgga acactgttat ttgaaaaaag gccttggcat gctttttgtc atagtctctt
     2161 gaatgcagtt ctagaaaaat agtgatccga acgataggtt ttggcctgtc gttcggatcg
     2221 ctttttcttt tccaactgta ttgctctttt attgaagata atttgctctt caatagatat
     2281 ttctagaaag gttctgttta tcccttactc aaaaaactgc tgcatttgtt ctcttacttg
     2341 ctgacaccat tggagttcct gctgacaaag cataattctg cgctggatca caacataacg
     2401 tccaaattct tgtttcttgt ctagcgagag gtcattcaat tttttttgat ttttttgcat
     2461 gattttgttc agctgttgtt ctctagtgaa tagcagtcca gtagctgttg ttttatcgag
     2521 cgtcgaaaaa gcatagattt ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc
     2581 ctctttgatc cattcctcta caagagcacg tccttcatca gtcaagtggt agatcttttt
     2641 ttgagagtgc tcgtctttgt ccaaaacggt cacataaccc tgctggtcta acttggcaag
     2701 cgttgtatag atctgactgt gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc
     2761 atacccagaa cgtggtttgg ataaaagcac gcataataat atataggcta aagtattcat
     2821 aaaaatccta cctctcgtct tattttcttt attatatcac agtaaagttt ccacgtttta
     2881 catgtgcata ttgacatgta aaataaatca tgtatgatgc taatagagag aaagggatgg
     2941 aaatattgga gaatcaaata gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa
     3001 gttcagcgct agttgtagca gctacctgta tgatcgcttt tatgggagtg ggtctagtag
     3061 acccaatctt aaaaacgatt gctgcacagt taaacgctac gccagctgaa acgaccctgt
     3121 tatttactag ttacatgtta gtcactggtg tggtgatgct atttacaggt ttcttatcta
     3181 gtcggatcgg tttgaaaaaa acattgatga caggattgtt tatcatcgtt gtattcgctg
     3241 gtcttggcgg attatctggg aatatcggaa tgctgattgg gttacgtgcc ggctggggtg
     3301 tcgggaatgc tttatttgtc tctactgctt tagcaacgat cgtcagtatt ttagttggta
     3361 acacagaaaa agcaatcatg atgtatgaag cagcacttgg ctgcggaatg gctgttggcc
     3421 ctctagtcgg tggggtcttg ggcagtggtt catggcgtta tccatttttt ggggtagcat
     3481 ttttgatgtt ttgcggtttt atagccctag cggtactttt acctgaaact gagaaaccaa
     3541 agagaaaagt aggcctttgg gaaggtgtca aagcgttaag caatcataaa cttcgttcag
     3601 ttggactaat cgcattgtta tataatttcg gattctttac tttacttgcg tatgcgccat
     3661 ttttattgat tggctattct gaattagaag tcggttttgt attttttggc tggggtgtgc
     3721 ttctggctat tgcttcgatt ttcgttgcac cggctcttga gcggaagttt acgacttatc
     3781 ggacaatttt agcagcattt gtcttattca ttatttgttt gctgttatta ggagctggaa
     3841 cttctatgcc agtccttgta ccgatcagca ttgtgttagc aggatttttc caaggaatta
     3901 tcaatacact gttgacgacg attgctatgg aaataccagg tctgcaacga aacgttgctt
     3961 cgtccagcta tagttttgtc cgctttttcg gtggagcttt agcaccattt attgcaggtg
     4021 tgattggtga acatattggt gaaagctatg cgttttattt tgcaggtgcc attgttctta
     4081 tcagtgtatg ttgtatcatt tatcaccgta attattttgc tacaaatgaa gaagaataag
     4141 aaatcagagg ttgtgaaaaa gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAZUMO010000242         3498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.147, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000242 DAZUMO010000000
VERSION     DAZUMO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.147"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1169
                     /locus_tag="ACG8ER_003045"
                     /pseudo
     CDS             49..1169
                     /locus_tag="ACG8ER_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            1427..2929
                     /gene="eat(A)"
                     /locus_tag="ACG8ER_003046"
     CDS             1427..2929
                     /gene="eat(A)"
                     /locus_tag="ACG8ER_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5051525.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2996..>3498
                     /locus_tag="ACG8ER_003047"
     CDS             2996..>3498
                     /locus_tag="ACG8ER_003047"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5051526.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVD"
BASE COUNT         1199 a          610 c          790 g          899 t
ORIGIN      
        1 aaaaaatgaa aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg
       61 gcggcaattg attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta
      121 aatggctggg aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa
      181 aatactgcag aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg
      241 gatctcttat tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac
      301 accgcagtca tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat
      361 caaactatcc gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt
      421 caagaagtag aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta
      481 ggattactcc aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta
      541 tctactaaaa aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt
      601 atcgctgcag atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga
      661 agacaaaaag gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt
      721 gctcagcaaa taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca
      781 gcaggcggta ttggtggagc ccttttttgt tgggagcgaa gatggtttct ggtttcgatt
      841 tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc atcattacag
      901 gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt gtagcagtgc
      961 ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac caagagctag
     1021 aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca ataggtttag
     1081 aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga cagacactta
     1141 gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta tgctatcgta
     1201 atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc gattgatgaa
     1261 agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt ttcgggattg
     1321 gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca tcctctttag
     1381 ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga
     1441 aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg aacaagcaaa
     1501 tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac
     1561 aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa tccatcagca
     1621 agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct attacgtgtt
     1681 aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat tgatgcaaac
     1741 agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga agacaaaagt
     1801 cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa
     1861 tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg
     1921 cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc atgttttagc
     1981 aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg aagaacagaa
     2041 aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag
     2101 gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag gagataaaac
     2161 aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag gagcagtggg
     2221 tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga tggagaccca
     2281 gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt tgacgatgaa
     2341 tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat tagggtatga
     2401 aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg tggcgatttc
     2461 aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg cattcaacgg
     2521 caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg caagccaaaa
     2581 ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag tagactacca
     2641 agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc ataacaagat
     2701 cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt tatcacagcc
     2761 agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca
     2821 attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac atgatcaaac
     2881 cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac ccttgtaatc
     2941 tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga atcacatgct
     3001 ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt gcggacgggc
     3061 agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag aaaaggtagt
     3121 agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag caagagatac
     3181 agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac gattggtaga
     3241 aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc aaaaagcacg
     3301 tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat
     3361 cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca
     3421 taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg aggggttctt
     3481 tacattcatg gtagataa
//
LOCUS       DAZUMO010000243         3008 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.153, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000243 DAZUMO010000000
VERSION     DAZUMO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.153"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            266..1234
                     /locus_tag="ACG8ER_003048"
     CDS             266..1234
                     /locus_tag="ACG8ER_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5051527.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1227..2258
                     /gene="vanA"
                     /locus_tag="ACG8ER_003049"
     CDS             1227..2258
                     /gene="vanA"
                     /locus_tag="ACG8ER_003049"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5051528.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2264..2872
                     /locus_tag="ACG8ER_003050"
     CDS             2264..2872
                     /locus_tag="ACG8ER_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS5051529.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          896 a          571 c          725 g          816 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa ggaggtccta agagatgtat
       61 ataatttttt aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta
      121 atattaagaa acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt
      181 cgttcttcag agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct
      241 ttcacagaaa gggttggtct taattatgaa taacatcggc attactgttt atggatgtga
      301 gcaggatgag gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat
      361 aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag
      421 tgtgggacat aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt
      481 gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag
      541 aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat
      601 gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca
      661 tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt
      721 gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt
      781 gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct
      841 gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat
      901 cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg
      961 tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc
     1021 cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc
     1081 aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca
     1141 tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg
     1201 tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg
     1261 gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta
     1321 ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt
     1381 gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg
     1441 ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg
     1501 atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt
     1561 ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg
     1621 acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg
     1681 ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc
     1741 cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact
     1801 acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg
     1861 gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg
     1921 accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag
     1981 gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga
     2041 tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg
     2101 atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt
     2161 tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac
     2221 tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac
     2281 ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa
     2341 tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt
     2401 ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct
     2461 atgggacggt taccgtccta agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc
     2521 ggaaaataac ctgacaaagg aaagttatta tcccaatatt gaccgaactg agatgatttc
     2581 aaaaggatac gtggcttcaa aatcaagcca tagccgcggc agtgccattg atcttacgct
     2641 ttatcgatta gacacgggtg agcttgtacc aatggggagc cgatttgatt ttatggatga
     2701 acgctctcat catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt
     2761 gcgctccatc atggaaaaca gtgggtttga agcatatagc ctcgaatggt ggcactatgt
     2821 attaagagac gaaccatacc ccaatagcta ttttgatttc cccgttaaat aaacttttaa
     2881 ccgttgcacg gacaaactat ataagctaac tctttcggca ggaaacccga cgtatgtaac
     2941 tggttcttag ggaatttata tatagtagat aacccgtggt gctagttaat tttaatatcc
     3001 taaagaat
//
LOCUS       DAZUMO010000244         2206 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.169, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000244 DAZUMO010000000
VERSION     DAZUMO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.169"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..192)
                     /locus_tag="ACG8ER_003051"
     CDS             complement(<1..192)
                     /locus_tag="ACG8ER_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051530.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSS"
     gene            285..634
                     /locus_tag="ACG8ER_003052"
                     /pseudo
     CDS             285..634
                     /locus_tag="ACG8ER_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(906..1196)
                     /locus_tag="ACG8ER_003053"
     CDS             complement(906..1196)
                     /locus_tag="ACG8ER_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5051531.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1404..2095)
                     /locus_tag="ACG8ER_003054"
                     /pseudo
     CDS             complement(1404..2095)
                     /locus_tag="ACG8ER_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          620 a          421 c          323 g          842 t
ORIGIN      
        1 tgaactccat gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct
       61 acttgcataa actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa
      121 gataccccaa atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt
      181 atgtttgttc atttttatac tccttcttat atttcctaat agagtataac atagctttaa
      241 gcattactta aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat
      301 ttgatgcaaa agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt
      361 tttcttagtt aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa
      421 gacatacaat catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa
      481 aatccaaatg ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc
      541 ttcaatttac ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca
      601 ggaacgttta aaatcctaca attccactac ctgagactag taatgactag tctcttttta
      661 tggcctctga tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc
      721 tttaagcaat atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa
      781 tacatgtagt tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc
      841 ccatcgtctg catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt
      901 gttttctagt ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca
      961 tattctacca caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt
     1021 ctattattat aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag
     1081 ttcaaactat gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt
     1141 ctggcactaa cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc
     1201 ttcacctcaa taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta
     1261 tctttttgac tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat
     1321 gaaggacatg tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat
     1381 ttaggtttac ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat
     1441 ttatcagctg tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc
     1501 aagcttgagt tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgattt
     1561 ctacatttgt tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat
     1621 tttgtaattc tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca
     1681 gatgattgat ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt
     1741 gtttgctttg tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg
     1801 ctgccgattg atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat
     1861 ctgcctttga atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa
     1921 tcgcttgctc tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat
     1981 caatatctgt ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc
     2041 caattagaca tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca
     2101 tagctcctta atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa
     2161 gcgtttttaa attacatatg taattttaat ataaatacta tcaaat
//
LOCUS       DAZUMO010000245         1531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.181, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000245 DAZUMO010000000
VERSION     DAZUMO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.181"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG8ER_003055"
     CDS             111..194
                     /locus_tag="ACG8ER_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS5051532.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8ER_003056"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8ER_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5051533.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1226..1486
                     /locus_tag="ACG8ER_003057"
                     /pseudo
     CDS             1226..1486
                     /locus_tag="ACG8ER_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          582 a          242 c          259 g          448 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1201 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1261 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
     1321 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
     1381 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
     1441 aattggggaa aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc
     1501 caaaagtagc tcctatatga aaaatagagg t
//
LOCUS       DAZUMO010000246         1521 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.182, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000246 DAZUMO010000000
VERSION     DAZUMO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.182"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1402
                     /locus_tag="ACG8ER_003058"
                     /note="aph(2'')-Ia"
     CDS             2..1402
                     /locus_tag="ACG8ER_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS5051534.1"
                     /translation="MDDDFPLMLKWLTDERVLEFYGGRDKKYTLESLKKHYTEPWEDE
                     VFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTR
                     YIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCY
                     LMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFST
                     NKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYST
                     MSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIE
                     KDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCD
                     FIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQE
                     FIENGRKEIYKRAYKD"
BASE COUNT          645 a          115 c          261 g          500 t
ORIGIN      
        1 aatagatgat gattttcctt tgatgttaaa atggttaact gatgaaagag tattagaatt
       61 ttatggtggt agagataaaa aatatacatt agaatcatta aaaaaacatt atacagagcc
      121 ttgggaagat gaagttttta gagtaattat tgaatataac aatgttccta ttggatatgg
      181 acaaatatat aaaatgtatg atgagttata tactgattat cattatccaa aaactgatga
      241 gatagtctat ggtatggatc aatttatagg agagccaaat tattggagta aaggaattgg
      301 tacaagatat attaaattga tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt
      361 tattttagac cctcataaaa ataatccaag agcaataagg gcataccaaa aatctggttt
      421 tagaattatt gaagatttgc cagaacatga attacacgag ggcaaaaaag aagattgtta
      481 tttaatggaa tatagatatg atgataatgc cacaaatgtt aaggcaatga aatatttaat
      541 tgagcattac tttgataatt tcaaagtaga tagtattgaa ataatcggta gtggttatga
      601 tagtgtggca tatttagtta ataatgaata catttttaaa acaaaattta gtactaataa
      661 gaaaaaaggt tatgcaaaag aaaaagcaat atataatttt ttaaatacaa atttagaaac
      721 taatgtaaaa attcctaata ttgaatattc gtatattagt gatgaattat ctatactagg
      781 ttataaagaa attaaaggaa cttttttaac accagaaatt tattctacta tgtcagaaga
      841 agaacaaaat ttgttaaaac gagatattgc cagtttttta agacaaatgc acggtttaga
      901 ttatacagat attagtgaat gtactattga taataaacaa aatgtattag aagagtatat
      961 attgttgcgt gaaactattt ataatgattt aactgatata gaaaaagatt atatagaaag
     1021 ttttatggaa agactaaatg caacaacagt ttttgagggt aaaaagtgtt tatgccataa
     1081 tgattttagt tgtaatcatc tattgttaga tggcaataat agattaactg gaataattga
     1141 ttttggagat tctggaatta tagatgaata ttgtgatttt atatacttac ttgaagatag
     1201 tgaagaagaa ataggaacaa attttggaga agatatatta agaatgtatg gaaatataga
     1261 tattgagaaa gcaaaagaat atcaagatat agttgaagaa tattatccta ttgaaactat
     1321 tgtttatgga attaaaaata ttaaacagga atttatcgaa aatggtagaa aagaaattta
     1381 taaaagggct tataaagatt gattatataa tatatgaaaa gctattataa aagacattag
     1441 tattaaatag tttaaaaaaa tgaaaaataa taaaggaagt gagtcaagtc cagactcctg
     1501 tgtaaaatgc tatacaatgt t
//
LOCUS       DAZUMO010000247          897 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5059
            SAMEA115707467-rid22570513.guided.211, whole genome shotgun
            sequence.
ACCESSION   DAZUMO010000247 DAZUMO010000000
VERSION     DAZUMO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707467
            Sequence Read Archive: ERR13197196
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288031.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288031.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:12:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 40 of 84
            Pseudo Genes (internal stop)      :: 15 of 84
            Pseudo Genes (multiple problems)  :: 9 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707467-rid22570513.guided.211"
                     /isolate="VRE5059"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            284..832
                     /gene="aac(6')"
                     /locus_tag="ACG8ER_003059"
     CDS             284..832
                     /gene="aac(6')"
                     /locus_tag="ACG8ER_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5051535.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          316 a          134 c          185 g          262 t
ORIGIN      
        1 tatggccttt ttacttttac acaattatac ggactttatc gtttttttta aagcaaaaga
       61 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
      121 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
      181 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
      241 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
      301 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
      361 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
      421 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
      481 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
      541 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
      601 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
      661 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
      721 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
      781 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
      841 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attactt
//
